rasgrf2a cDNA ORF clone, Danio rerio(zebrafish)

The following rasgrf2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rasgrf2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145216 XM_021472384.1
Latest version!
Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa42925 XM_009305082.3
Latest version!
Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa42926 XM_009305083.3
Latest version!
Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa42926 XM_009305083.2 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa42925 XM_009305082.2 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145216
    Clone ID Related Accession (Same CDS sequence) XM_021472384.1
    Accession Version XM_021472384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-specific guanine nucleotide-releasing factor 2-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394799.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGATGG ACAAAAATGA AAGAACTCCA TACATTATGA AGACCAGTCA GCACTTCAAT 
    GATATGAGTA ATCTGGTAGC TTCTGAGATT ATGGCTCATG CAGATGTGAG CTCCAGGGCT
    GGATCCATTG ACAAGTGGCT GGCAGTGGCT GATATCTGCC GCTGTCTCAA CAACTACAAC
    GGAGTCTTGG AAATCACATC TGCACTGAAC CGCAGCGCCA TCTACAGGCT GAAGAAGACA
    TGGGCCAAGG TTTGCAAACA GACCAAGGCA TTAATGGATA GGCTGCAGAA GATCGTGTCG
    TCTGAAGGGA GGTTTAAGAA CCTGAGGGAA ACTCTGAAGA ACTGTAACCC ACCGTGTGTG
    CCCTATCTGG GCATGTACCT GACAGATTTG GCATTTATCG AGGAAGGAAC ACCCAATTTC
    ACGGAGGAGG GTCTCGTCAA CTTCTCTAAA ATGAGGATGA TTTCACACAT AATACGAGAG
    ATTCGACAGT TTCAGCAGGC GCCCTATAGG ATTGAACTGC AACCTAAGGT GACACAGTTC
    CTCCTGGATA AGACCCTTGT AATGGACGAG GACACGCTTT ATGAACTATC TCTGAAGATC
    GAGCCAAGAC TTCCACCTGC CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328059.1
    CDS42..668
    Translation

    Target ORF information:

    RefSeq Version XM_021472384.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGATGG ACAAAAATGA AAGAACTCCA TACATTATGA AGACCAGTCA GCACTTCAAT 
    GATATGAGTA ATCTGGTAGC TTCTGAGATT ATGGCTCATG CAGATGTGAG CTCCAGGGCT
    GGATCCATTG ACAAGTGGCT GGCAGTGGCT GATATCTGCC GCTGTCTCAA CAACTACAAC
    GGAGTCTTGG AAATCACATC TGCACTGAAC CGCAGCGCCA TCTACAGGCT GAAGAAGACA
    TGGGCCAAGG TTTGCAAACA GACCAAGGCA TTAATGGATA GGCTGCAGAA GATCGTGTCG
    TCTGAAGGGA GGTTTAAGAA CCTGAGGGAA ACTCTGAAGA ACTGTAACCC ACCGTGTGTG
    CCCTATCTGG GCATGTACCT GACAGATTTG GCATTTATCG AGGAAGGAAC ACCCAATTTC
    ACGGAGGAGG GTCTCGTCAA CTTCTCTAAA ATGAGGATGA TTTCACACAT AATACGAGAG
    ATTCGACAGT TTCAGCAGGC GCCCTATAGG ATTGAACTGC AACCTAAGGT GACACAGTTC
    CTCCTGGATA AGACCCTTGT AATGGACGAG GACACGCTTT ATGAACTATC TCTGAAGATC
    GAGCCAAGAC TTCCACCTGC CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42925
    Clone ID Related Accession (Same CDS sequence) XM_009305082.2 , XM_009305082.3
    Accession Version XM_009305082.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-specific guanine nucleotide-releasing factor 2-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGG TTCAGTATCA TTCATCTGAC
    CGTTTGTCCA TCACCTCCAT CTGTCCAGAC TACAGCCTGA AGATCACGAG GCTTGCGCTG
    GACCAGTCCA AGTCCCTGGA GCTTTTCTTC GCCACCAACC AGGGCCCCTG GAGCGGAGAG
    CATCTCAACA ACAAATCCCC CCGGCTGTCC CGCAAGTTCT CCTCACCTCC GCCCATCTCC
    ATCACCTCGC GCACCTCCTC CCCCATCCAC TCCCGCAAGC TGTCCCTCAG CTCTCCTGTC
    AGCTGTAAGG CTGGAGCTCT GGATCTCTCC ACCTCCACCT TCTCTGCAGC CAGCAGTCCA
    ACATCTCCAT ATAACCCCAT CATCTGCTCT CCTCCGCCCA CCTGCACCAA AGCCCCTCTG
    GACCTCAGCC GGGGCCCCAG CTCCCCGGAG CTCTGCCCCT CCACCCCGGA GGAAGGTGGA
    GAACTGCCCC GCATAGATGC TTTCTGTGGG AAACTGCGGC GGAGTATTCG GCGAGCTGTA
    CTTGAGTCAG TATCACTGGA CAAGTTCATT CCAGAGACAC CCACATCCAG TGACTCTGGA
    GAAATGTCTC CAAGCCGTTC CCCATCCACC CCGAGACACC TTCGCTACCG ACAGCCCGCA
    GGTCAGTGTC CTGATAACTC CCGCTGTGCC GTGTCCCCCG CGTCCGCCTT CGCCATTGCC
    ACCGCCGCAG CCGGACACAG CAGCCCGCAA GGATTTACCA ACCCAGAGAA ATCCTACGAC
    AAAGAGTTCC TCATCCGCAG GGCGGCCACC AACAGAGTGC TCAACGTCCT GCGCCACTGG
    GTTTCTAAAC ACTCGCAGGA CTTTGAGATG AACGCAGAGC TGACGACTGC TGTGATGGCT
    CTGCTGGAGG AGGTGCTGAG AGATCCAGAT CTTCTGCCGC AGGAGAGAAA AGCTGCAGTC
    AATATACTGA GTGCGCTTTC TCAGGAAGAA CAAGACGATT CACAATTGAA GATTGAGGAT
    ATCGTCCAAA TGGTGGACTG TCCGAGGGCC GAATGCTTTG AGTCACTATC TGCAATGGAG
    CTAGCAGAAC AAATCACCCT GCTGGACCAT ATTGTGTTCC GGAATATTCC CTATGAGGAG
    TTTCTTGGTC AAGGCTGGAT GAAGATGGAC AAAAATGAAA GAACTCCATA CATTATGAAG
    ACCAGTCAGC ACTTCAATGA TATGAGTAAT CTGGTAGCTT CTGAGATTAT GGCTCATGCA
    GATGTGAGCT CCAGGGCTGG ATCCATTGAC AAGTGGCTGG CAGTGGCTGA TATCTGCCGC
    TGTCTCAACA ACTACAACGG AGTCTTGGAA ATCACATCTG CACTGAACCG CAGCGCCATC
    TACAGGCTGA AGAAGACATG GGCCAAGGTT TGCAAACAGA CCAAGGCATT AATGGATAGG
    CTGCAGAAGA TCGTGTCGTC TGAAGGGAGG TTTAAGAACC TGAGGGAAAC TCTGAAGAAC
    TGTAACCCAC CGTGTGTGCC CTATCTGGGC ATGTACCTGA CAGATTTGGC ATTTATCGAG
    GAAGGAACAC CCAATTTCAC GGAGGAGGGT CTCGTCAACT TCTCTAAAAT GAGGATGATT
    TCACACATAA TACGAGAGAT TCGACAGTTT CAGCAGGCGC CCTATAGGAT TGAACTGCAA
    CCTAAGGTGA CACAGTTCCT CCTGGATAAG ACCCTTGTAA TGGACGAGGA CACGCTTTAT
    GAACTATCTC TGAAGATCGA GCCAAGACTT CCACCTGCCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303357.1
    CDS298..4122
    Translation

    Target ORF information:

    RefSeq Version XM_009305082.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGG TTCAGTATCA TTCATCTGAC
    CGTTTGTCCA TCACCTCCAT CTGTCCAGAC TACAGCCTGA AGATCACGAG GCTTGCGCTG
    GACCAGTCCA AGTCCCTGGA GCTTTTCTTC GCCACCAACC AGGGCCCCTG GAGCGGAGAG
    CATCTCAACA ACAAATCCCC CCGGCTGTCC CGCAAGTTCT CCTCACCTCC GCCCATCTCC
    ATCACCTCGC GCACCTCCTC CCCCATCCAC TCCCGCAAGC TGTCCCTCAG CTCTCCTGTC
    AGCTGTAAGG CTGGAGCTCT GGATCTCTCC ACCTCCACCT TCTCTGCAGC CAGCAGTCCA
    ACATCTCCAT ATAACCCCAT CATCTGCTCT CCTCCGCCCA CCTGCACCAA AGCCCCTCTG
    GACCTCAGCC GGGGCCCCAG CTCCCCGGAG CTCTGCCCCT CCACCCCGGA GGAAGGTGGA
    GAACTGCCCC GCATAGATGC TTTCTGTGGG AAACTGCGGC GGAGTATTCG GCGAGCTGTA
    CTTGAGTCAG TATCACTGGA CAAGTTCATT CCAGAGACAC CCACATCCAG TGACTCTGGA
    GAAATGTCTC CAAGCCGTTC CCCATCCACC CCGAGACACC TTCGCTACCG ACAGCCCGCA
    GGTCAGTGTC CTGATAACTC CCGCTGTGCC GTGTCCCCCG CGTCCGCCTT CGCCATTGCC
    ACCGCCGCAG CCGGACACAG CAGCCCGCAA GGATTTACCA ACCCAGAGAA ATCCTACGAC
    AAAGAGTTCC TCATCCGCAG GGCGGCCACC AACAGAGTGC TCAACGTCCT GCGCCACTGG
    GTTTCTAAAC ACTCGCAGGA CTTTGAGATG AACGCAGAGC TGACGACTGC TGTGATGGCT
    CTGCTGGAGG AGGTGCTGAG AGATCCAGAT CTTCTGCCGC AGGAGAGAAA AGCTGCAGTC
    AATATACTGA GTGCGCTTTC TCAGGAAGAA CAAGACGATT CACAATTGAA GATTGAGGAT
    ATCGTCCAAA TGGTGGACTG TCCGAGGGCC GAATGCTTTG AGTCACTATC TGCAATGGAG
    CTAGCAGAAC AAATCACCCT GCTGGACCAT ATTGTGTTCC GGAATATTCC CTATGAGGAG
    TTTCTTGGTC AAGGCTGGAT GAAGATGGAC AAAAATGAAA GAACTCCATA CATTATGAAG
    ACCAGTCAGC ACTTCAATGA TATGAGTAAT CTGGTAGCTT CTGAGATTAT GGCTCATGCA
    GATGTGAGCT CCAGGGCTGG ATCCATTGAC AAGTGGCTGG CAGTGGCTGA TATCTGCCGC
    TGTCTCAACA ACTACAACGG AGTCTTGGAA ATCACATCTG CACTGAACCG CAGCGCCATC
    TACAGGCTGA AGAAGACATG GGCCAAGGTT TGCAAACAGA CCAAGGCATT AATGGATAGG
    CTGCAGAAGA TCGTGTCGTC TGAAGGGAGG TTTAAGAACC TGAGGGAAAC TCTGAAGAAC
    TGTAACCCAC CGTGTGTGCC CTATCTGGGC ATGTACCTGA CAGATTTGGC ATTTATCGAG
    GAAGGAACAC CCAATTTCAC GGAGGAGGGT CTCGTCAACT TCTCTAAAAT GAGGATGATT
    TCACACATAA TACGAGAGAT TCGACAGTTT CAGCAGGCGC CCTATAGGAT TGAACTGCAA
    CCTAAGGTGA CACAGTTCCT CCTGGATAAG ACCCTTGTAA TGGACGAGGA CACGCTTTAT
    GAACTATCTC TGAAGATCGA GCCAAGACTT CCACCTGCCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42925
    Clone ID Related Accession (Same CDS sequence) XM_009305082.2 , XM_009305082.3
    Accession Version XM_009305082.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-specific guanine nucleotide-releasing factor 2-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGG TTCAGTATCA TTCATCTGAC
    CGTTTGTCCA TCACCTCCAT CTGTCCAGAC TACAGCCTGA AGATCACGAG GCTTGCGCTG
    GACCAGTCCA AGTCCCTGGA GCTTTTCTTC GCCACCAACC AGGGCCCCTG GAGCGGAGAG
    CATCTCAACA ACAAATCCCC CCGGCTGTCC CGCAAGTTCT CCTCACCTCC GCCCATCTCC
    ATCACCTCGC GCACCTCCTC CCCCATCCAC TCCCGCAAGC TGTCCCTCAG CTCTCCTGTC
    AGCTGTAAGG CTGGAGCTCT GGATCTCTCC ACCTCCACCT TCTCTGCAGC CAGCAGTCCA
    ACATCTCCAT ATAACCCCAT CATCTGCTCT CCTCCGCCCA CCTGCACCAA AGCCCCTCTG
    GACCTCAGCC GGGGCCCCAG CTCCCCGGAG CTCTGCCCCT CCACCCCGGA GGAAGGTGGA
    GAACTGCCCC GCATAGATGC TTTCTGTGGG AAACTGCGGC GGAGTATTCG GCGAGCTGTA
    CTTGAGTCAG TATCACTGGA CAAGTTCATT CCAGAGACAC CCACATCCAG TGACTCTGGA
    GAAATGTCTC CAAGCCGTTC CCCATCCACC CCGAGACACC TTCGCTACCG ACAGCCCGCA
    GGTCAGTGTC CTGATAACTC CCGCTGTGCC GTGTCCCCCG CGTCCGCCTT CGCCATTGCC
    ACCGCCGCAG CCGGACACAG CAGCCCGCAA GGATTTACCA ACCCAGAGAA ATCCTACGAC
    AAAGAGTTCC TCATCCGCAG GGCGGCCACC AACAGAGTGC TCAACGTCCT GCGCCACTGG
    GTTTCTAAAC ACTCGCAGGA CTTTGAGATG AACGCAGAGC TGACGACTGC TGTGATGGCT
    CTGCTGGAGG AGGTGCTGAG AGATCCAGAT CTTCTGCCGC AGGAGAGAAA AGCTGCAGTC
    AATATACTGA GTGCGCTTTC TCAGGAAGAA CAAGACGATT CACAATTGAA GATTGAGGAT
    ATCGTCCAAA TGGTGGACTG TCCGAGGGCC GAATGCTTTG AGTCACTATC TGCAATGGAG
    CTAGCAGAAC AAATCACCCT GCTGGACCAT ATTGTGTTCC GGAATATTCC CTATGAGGAG
    TTTCTTGGTC AAGGCTGGAT GAAGATGGAC AAAAATGAAA GAACTCCATA CATTATGAAG
    ACCAGTCAGC ACTTCAATGA TATGAGTAAT CTGGTAGCTT CTGAGATTAT GGCTCATGCA
    GATGTGAGCT CCAGGGCTGG ATCCATTGAC AAGTGGCTGG CAGTGGCTGA TATCTGCCGC
    TGTCTCAACA ACTACAACGG AGTCTTGGAA ATCACATCTG CACTGAACCG CAGCGCCATC
    TACAGGCTGA AGAAGACATG GGCCAAGGTT TGCAAACAGA CCAAGGCATT AATGGATAGG
    CTGCAGAAGA TCGTGTCGTC TGAAGGGAGG TTTAAGAACC TGAGGGAAAC TCTGAAGAAC
    TGTAACCCAC CGTGTGTGCC CTATCTGGGC ATGTACCTGA CAGATTTGGC ATTTATCGAG
    GAAGGAACAC CCAATTTCAC GGAGGAGGGT CTCGTCAACT TCTCTAAAAT GAGGATGATT
    TCACACATAA TACGAGAGAT TCGACAGTTT CAGCAGGCGC CCTATAGGAT TGAACTGCAA
    CCTAAGGTGA CACAGTTCCT CCTGGATAAG ACCCTTGTAA TGGACGAGGA CACGCTTTAT
    GAACTATCTC TGAAGATCGA GCCAAGACTT CCACCTGCCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303357.1
    CDS308..4132
    Translation

    Target ORF information:

    RefSeq Version XM_009305082.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305082.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGG TTCAGTATCA TTCATCTGAC
    CGTTTGTCCA TCACCTCCAT CTGTCCAGAC TACAGCCTGA AGATCACGAG GCTTGCGCTG
    GACCAGTCCA AGTCCCTGGA GCTTTTCTTC GCCACCAACC AGGGCCCCTG GAGCGGAGAG
    CATCTCAACA ACAAATCCCC CCGGCTGTCC CGCAAGTTCT CCTCACCTCC GCCCATCTCC
    ATCACCTCGC GCACCTCCTC CCCCATCCAC TCCCGCAAGC TGTCCCTCAG CTCTCCTGTC
    AGCTGTAAGG CTGGAGCTCT GGATCTCTCC ACCTCCACCT TCTCTGCAGC CAGCAGTCCA
    ACATCTCCAT ATAACCCCAT CATCTGCTCT CCTCCGCCCA CCTGCACCAA AGCCCCTCTG
    GACCTCAGCC GGGGCCCCAG CTCCCCGGAG CTCTGCCCCT CCACCCCGGA GGAAGGTGGA
    GAACTGCCCC GCATAGATGC TTTCTGTGGG AAACTGCGGC GGAGTATTCG GCGAGCTGTA
    CTTGAGTCAG TATCACTGGA CAAGTTCATT CCAGAGACAC CCACATCCAG TGACTCTGGA
    GAAATGTCTC CAAGCCGTTC CCCATCCACC CCGAGACACC TTCGCTACCG ACAGCCCGCA
    GGTCAGTGTC CTGATAACTC CCGCTGTGCC GTGTCCCCCG CGTCCGCCTT CGCCATTGCC
    ACCGCCGCAG CCGGACACAG CAGCCCGCAA GGATTTACCA ACCCAGAGAA ATCCTACGAC
    AAAGAGTTCC TCATCCGCAG GGCGGCCACC AACAGAGTGC TCAACGTCCT GCGCCACTGG
    GTTTCTAAAC ACTCGCAGGA CTTTGAGATG AACGCAGAGC TGACGACTGC TGTGATGGCT
    CTGCTGGAGG AGGTGCTGAG AGATCCAGAT CTTCTGCCGC AGGAGAGAAA AGCTGCAGTC
    AATATACTGA GTGCGCTTTC TCAGGAAGAA CAAGACGATT CACAATTGAA GATTGAGGAT
    ATCGTCCAAA TGGTGGACTG TCCGAGGGCC GAATGCTTTG AGTCACTATC TGCAATGGAG
    CTAGCAGAAC AAATCACCCT GCTGGACCAT ATTGTGTTCC GGAATATTCC CTATGAGGAG
    TTTCTTGGTC AAGGCTGGAT GAAGATGGAC AAAAATGAAA GAACTCCATA CATTATGAAG
    ACCAGTCAGC ACTTCAATGA TATGAGTAAT CTGGTAGCTT CTGAGATTAT GGCTCATGCA
    GATGTGAGCT CCAGGGCTGG ATCCATTGAC AAGTGGCTGG CAGTGGCTGA TATCTGCCGC
    TGTCTCAACA ACTACAACGG AGTCTTGGAA ATCACATCTG CACTGAACCG CAGCGCCATC
    TACAGGCTGA AGAAGACATG GGCCAAGGTT TGCAAACAGA CCAAGGCATT AATGGATAGG
    CTGCAGAAGA TCGTGTCGTC TGAAGGGAGG TTTAAGAACC TGAGGGAAAC TCTGAAGAAC
    TGTAACCCAC CGTGTGTGCC CTATCTGGGC ATGTACCTGA CAGATTTGGC ATTTATCGAG
    GAAGGAACAC CCAATTTCAC GGAGGAGGGT CTCGTCAACT TCTCTAAAAT GAGGATGATT
    TCACACATAA TACGAGAGAT TCGACAGTTT CAGCAGGCGC CCTATAGGAT TGAACTGCAA
    CCTAAGGTGA CACAGTTCCT CCTGGATAAG ACCCTTGTAA TGGACGAGGA CACGCTTTAT
    GAACTATCTC TGAAGATCGA GCCAAGACTT CCACCTGCCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42926
    Clone ID Related Accession (Same CDS sequence) XM_009305083.3 , XM_009305083.2
    Accession Version XM_009305083.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ras-specific guanine nucleotide-releasing factor 2-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGT CCCTGGAGCT TTTCTTCGCC
    ACCAACCAGG GCCCCTGGAG CGGAGAGCAT CTCAACAACA AATCCCCCCG GCTGTCCCGC
    AAGTTCTCCT CACCTCCGCC CATCTCCATC ACCTCGCGCA CCTCCTCCCC CATCCACTCC
    CGCAAGCTGT CCCTCAGCTC TCCTGTCAGC TGTAAGGCTG GAGCTCTGGA TCTCTCCACC
    TCCACCTTCT CTGCAGCCAG CAGTCCAACA TCTCCATATA ACCCCATCAT CTGCTCTCCT
    CCGCCCACCT GCACCAAAGC CCCTCTGGAC CTCAGCCGGG GCCCCAGCTC CCCGGAGCTC
    TGCCCCTCCA CCCCGGAGGA AGGTGGAGAA CTGCCCCGCA TAGATGCTTT CTGTGGGAAA
    CTGCGGCGGA GTATTCGGCG AGCTGTACTT GAGTCAGTAT CACTGGACAA GTTCATTCCA
    GAGACACCCA CATCCAGTGA CTCTGGAGAA ATGTCTCCAA GCCGTTCCCC ATCCACCCCG
    AGACACCTTC GCTACCGACA GCCCGCAGGT CAGTGTCCTG ATAACTCCCG CTGTGCCGTG
    TCCCCCGCGT CCGCCTTCGC CATTGCCACC GCCGCAGCCG GACACAGCAG CCCGCAAGGA
    TTTACCAACC CAGAGAAATC CTACGACAAA GAGTTCCTCA TCCGCAGGGC GGCCACCAAC
    AGAGTGCTCA ACGTCCTGCG CCACTGGGTT TCTAAACACT CGCAGGACTT TGAGATGAAC
    GCAGAGCTGA CGACTGCTGT GATGGCTCTG CTGGAGGAGG TGCTGAGAGA TCCAGATCTT
    CTGCCGCAGG AGAGAAAAGC TGCAGTCAAT ATACTGAGTG CGCTTTCTCA GGAAGAACAA
    GACGATTCAC AATTGAAGAT TGAGGATATC GTCCAAATGG TGGACTGTCC GAGGGCCGAA
    TGCTTTGAGT CACTATCTGC AATGGAGCTA GCAGAACAAA TCACCCTGCT GGACCATATT
    GTGTTCCGGA ATATTCCCTA TGAGGAGTTT CTTGGTCAAG GCTGGATGAA GATGGACAAA
    AATGAAAGAA CTCCATACAT TATGAAGACC AGTCAGCACT TCAATGATAT GAGTAATCTG
    GTAGCTTCTG AGATTATGGC TCATGCAGAT GTGAGCTCCA GGGCTGGATC CATTGACAAG
    TGGCTGGCAG TGGCTGATAT CTGCCGCTGT CTCAACAACT ACAACGGAGT CTTGGAAATC
    ACATCTGCAC TGAACCGCAG CGCCATCTAC AGGCTGAAGA AGACATGGGC CAAGGTTTGC
    AAACAGACCA AGGCATTAAT GGATAGGCTG CAGAAGATCG TGTCGTCTGA AGGGAGGTTT
    AAGAACCTGA GGGAAACTCT GAAGAACTGT AACCCACCGT GTGTGCCCTA TCTGGGCATG
    TACCTGACAG ATTTGGCATT TATCGAGGAA GGAACACCCA ATTTCACGGA GGAGGGTCTC
    GTCAACTTCT CTAAAATGAG GATGATTTCA CACATAATAC GAGAGATTCG ACAGTTTCAG
    CAGGCGCCCT ATAGGATTGA ACTGCAACCT AAGGTGACAC AGTTCCTCCT GGATAAGACC
    CTTGTAATGG ACGAGGACAC GCTTTATGAA CTATCTCTGA AGATCGAGCC AAGACTTCCA
    CCTGCCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303358.1
    CDS308..4039
    Translation

    Target ORF information:

    RefSeq Version XM_009305083.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305083.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGT CCCTGGAGCT TTTCTTCGCC
    ACCAACCAGG GCCCCTGGAG CGGAGAGCAT CTCAACAACA AATCCCCCCG GCTGTCCCGC
    AAGTTCTCCT CACCTCCGCC CATCTCCATC ACCTCGCGCA CCTCCTCCCC CATCCACTCC
    CGCAAGCTGT CCCTCAGCTC TCCTGTCAGC TGTAAGGCTG GAGCTCTGGA TCTCTCCACC
    TCCACCTTCT CTGCAGCCAG CAGTCCAACA TCTCCATATA ACCCCATCAT CTGCTCTCCT
    CCGCCCACCT GCACCAAAGC CCCTCTGGAC CTCAGCCGGG GCCCCAGCTC CCCGGAGCTC
    TGCCCCTCCA CCCCGGAGGA AGGTGGAGAA CTGCCCCGCA TAGATGCTTT CTGTGGGAAA
    CTGCGGCGGA GTATTCGGCG AGCTGTACTT GAGTCAGTAT CACTGGACAA GTTCATTCCA
    GAGACACCCA CATCCAGTGA CTCTGGAGAA ATGTCTCCAA GCCGTTCCCC ATCCACCCCG
    AGACACCTTC GCTACCGACA GCCCGCAGGT CAGTGTCCTG ATAACTCCCG CTGTGCCGTG
    TCCCCCGCGT CCGCCTTCGC CATTGCCACC GCCGCAGCCG GACACAGCAG CCCGCAAGGA
    TTTACCAACC CAGAGAAATC CTACGACAAA GAGTTCCTCA TCCGCAGGGC GGCCACCAAC
    AGAGTGCTCA ACGTCCTGCG CCACTGGGTT TCTAAACACT CGCAGGACTT TGAGATGAAC
    GCAGAGCTGA CGACTGCTGT GATGGCTCTG CTGGAGGAGG TGCTGAGAGA TCCAGATCTT
    CTGCCGCAGG AGAGAAAAGC TGCAGTCAAT ATACTGAGTG CGCTTTCTCA GGAAGAACAA
    GACGATTCAC AATTGAAGAT TGAGGATATC GTCCAAATGG TGGACTGTCC GAGGGCCGAA
    TGCTTTGAGT CACTATCTGC AATGGAGCTA GCAGAACAAA TCACCCTGCT GGACCATATT
    GTGTTCCGGA ATATTCCCTA TGAGGAGTTT CTTGGTCAAG GCTGGATGAA GATGGACAAA
    AATGAAAGAA CTCCATACAT TATGAAGACC AGTCAGCACT TCAATGATAT GAGTAATCTG
    GTAGCTTCTG AGATTATGGC TCATGCAGAT GTGAGCTCCA GGGCTGGATC CATTGACAAG
    TGGCTGGCAG TGGCTGATAT CTGCCGCTGT CTCAACAACT ACAACGGAGT CTTGGAAATC
    ACATCTGCAC TGAACCGCAG CGCCATCTAC AGGCTGAAGA AGACATGGGC CAAGGTTTGC
    AAACAGACCA AGGCATTAAT GGATAGGCTG CAGAAGATCG TGTCGTCTGA AGGGAGGTTT
    AAGAACCTGA GGGAAACTCT GAAGAACTGT AACCCACCGT GTGTGCCCTA TCTGGGCATG
    TACCTGACAG ATTTGGCATT TATCGAGGAA GGAACACCCA ATTTCACGGA GGAGGGTCTC
    GTCAACTTCT CTAAAATGAG GATGATTTCA CACATAATAC GAGAGATTCG ACAGTTTCAG
    CAGGCGCCCT ATAGGATTGA ACTGCAACCT AAGGTGACAC AGTTCCTCCT GGATAAGACC
    CTTGTAATGG ACGAGGACAC GCTTTATGAA CTATCTCTGA AGATCGAGCC AAGACTTCCA
    CCTGCCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42926
    Clone ID Related Accession (Same CDS sequence) XM_009305083.3 , XM_009305083.2
    Accession Version XM_009305083.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ras-specific guanine nucleotide-releasing factor 2-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGT CCCTGGAGCT TTTCTTCGCC
    ACCAACCAGG GCCCCTGGAG CGGAGAGCAT CTCAACAACA AATCCCCCCG GCTGTCCCGC
    AAGTTCTCCT CACCTCCGCC CATCTCCATC ACCTCGCGCA CCTCCTCCCC CATCCACTCC
    CGCAAGCTGT CCCTCAGCTC TCCTGTCAGC TGTAAGGCTG GAGCTCTGGA TCTCTCCACC
    TCCACCTTCT CTGCAGCCAG CAGTCCAACA TCTCCATATA ACCCCATCAT CTGCTCTCCT
    CCGCCCACCT GCACCAAAGC CCCTCTGGAC CTCAGCCGGG GCCCCAGCTC CCCGGAGCTC
    TGCCCCTCCA CCCCGGAGGA AGGTGGAGAA CTGCCCCGCA TAGATGCTTT CTGTGGGAAA
    CTGCGGCGGA GTATTCGGCG AGCTGTACTT GAGTCAGTAT CACTGGACAA GTTCATTCCA
    GAGACACCCA CATCCAGTGA CTCTGGAGAA ATGTCTCCAA GCCGTTCCCC ATCCACCCCG
    AGACACCTTC GCTACCGACA GCCCGCAGGT CAGTGTCCTG ATAACTCCCG CTGTGCCGTG
    TCCCCCGCGT CCGCCTTCGC CATTGCCACC GCCGCAGCCG GACACAGCAG CCCGCAAGGA
    TTTACCAACC CAGAGAAATC CTACGACAAA GAGTTCCTCA TCCGCAGGGC GGCCACCAAC
    AGAGTGCTCA ACGTCCTGCG CCACTGGGTT TCTAAACACT CGCAGGACTT TGAGATGAAC
    GCAGAGCTGA CGACTGCTGT GATGGCTCTG CTGGAGGAGG TGCTGAGAGA TCCAGATCTT
    CTGCCGCAGG AGAGAAAAGC TGCAGTCAAT ATACTGAGTG CGCTTTCTCA GGAAGAACAA
    GACGATTCAC AATTGAAGAT TGAGGATATC GTCCAAATGG TGGACTGTCC GAGGGCCGAA
    TGCTTTGAGT CACTATCTGC AATGGAGCTA GCAGAACAAA TCACCCTGCT GGACCATATT
    GTGTTCCGGA ATATTCCCTA TGAGGAGTTT CTTGGTCAAG GCTGGATGAA GATGGACAAA
    AATGAAAGAA CTCCATACAT TATGAAGACC AGTCAGCACT TCAATGATAT GAGTAATCTG
    GTAGCTTCTG AGATTATGGC TCATGCAGAT GTGAGCTCCA GGGCTGGATC CATTGACAAG
    TGGCTGGCAG TGGCTGATAT CTGCCGCTGT CTCAACAACT ACAACGGAGT CTTGGAAATC
    ACATCTGCAC TGAACCGCAG CGCCATCTAC AGGCTGAAGA AGACATGGGC CAAGGTTTGC
    AAACAGACCA AGGCATTAAT GGATAGGCTG CAGAAGATCG TGTCGTCTGA AGGGAGGTTT
    AAGAACCTGA GGGAAACTCT GAAGAACTGT AACCCACCGT GTGTGCCCTA TCTGGGCATG
    TACCTGACAG ATTTGGCATT TATCGAGGAA GGAACACCCA ATTTCACGGA GGAGGGTCTC
    GTCAACTTCT CTAAAATGAG GATGATTTCA CACATAATAC GAGAGATTCG ACAGTTTCAG
    CAGGCGCCCT ATAGGATTGA ACTGCAACCT AAGGTGACAC AGTTCCTCCT GGATAAGACC
    CTTGTAATGG ACGAGGACAC GCTTTATGAA CTATCTCTGA AGATCGAGCC AAGACTTCCA
    CCTGCCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303358.1
    CDS298..4029
    Translation

    Target ORF information:

    RefSeq Version XM_009305083.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2a (rasgrf2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGTTA TAATGAAGGA CACGCGCTCT ATCTGTCCAT GGTCGCGCGT 
    AAAGAGGGAA TCAAGCGCGG GCACCTGAGC AAAAAAGCCG CCGAGAACAA CCGCTGGAAC
    GAGAAGTACT TCGCGCTTTA CCAGAATGTG CTGTTTTACT TTGAGAACGA GCAGAGCACC
    AGACCCTCTG GGATTTATTT GCTGGAGGGA AGCACCTGCG AGAGGATTCC GGCTCCGAAA
    GTGTCCACCG TGGGGAAGGA GAGTTCAGAT AAACAGCAGC ATTACTTCTT GGTGATTTTC
    GGCCACGATG GGCAGAAACC TCTGGAGCTG CGGTCAGAAG AAGAAGCTGA TTGTAATGAG
    TGGGTGGAGG TCATTCAGCA GGCCAGTTAT TCAGATCTGA TCATCGAGCG GGAAGTTCTC
    ATGCAGAAAT ACATCCATCT GGTGCAGATC ATGGAGACGG AAAAGGTGGC GGCAAATCAG
    CTCCGGACTC AACTGGAGGA CCAGGATACA GAGATCGAGA GGCTGAAGGC CGAGATTGTT
    GTTCTGAACA AATCAAAAGA GCGGATGTTG CCCTTTCAGT CCTTTCAAGA CGAAGAAGAT
    CCAGACATCA AAAAAATCAA GATGGTGCAG AGCTTCATGC GTGGCTGGCT CTGCAGGAGG
    AAGTGGAAGA TCATCGTGCA GGACTACATC TGCTCGCCTC ACGCCGAGAG CATGAGGAAG
    AGGAACCAGA TCGTCTTTAA CATGGTGGAG GCCGAGACGG AGTACGTGCT CCAGCTCTCC
    ATCCTGGTCA ACTGCTTCCT CCGGCCGCTC CGGATGGCGG CCAGCTCCAA AAAACCTCCC
    ATCAGTCACG ACGATGTCAG CAGCATCTTC CTCAATAGTG AGACGATAAT GTTTCTCCAT
    GAGATATTTC ACCAAGGCTT GAAGGCTCGG ATAGCCAACT GGCCGACGTT AGTTTTAGCG
    GATCTCTTCG ACATCTTGCT GCCAATGCTG AACATTTATC AGGAGTTTGT GCGAAACCAT
    CAGTACAGTT TGCAGGTTCT GGCCAACTGT AAACAGAACC GAGACTTCGA CAAGCTTCTG
    AAGCAGTATG AAGCAAACGC CGCCTGTGAG GGCCGGATGC TGGAGACCTT TCTCACATAC
    CCCATGTTTC AGATCCCACG ATACATCATC ACGCTCCATG AGCTGCTCGC TCACACGCCT
    CATGAACATG TGGAGCGCAA GAGTCTGGAA TTTGCCAAAT CCAAACTAGA AGAACTGTCC
    AAAATGATGC ACGATGAAGT GAGTGACACA GAAAACATCC GTAAGAATCT AGCGATCGAG
    CGAATGATTG TGGAGGGCTG TGATATCCTG CTGGACACCA GTCAGACGTT TGTAAGGCAG
    GGCTCTCTCA TTCAGCTGCC CTCTGTGGAG AAGGGTAAAC TGAGTAAAGT GCGTCTGGGC
    TCGCTGACGC TCAGGAAAGA AGCTGAACGA CAGTGTTTCC TCTTCACCAA ACACTTCCTC
    ATCTGCACAC GGACCTCCGG CGGCAAACTG CACCTGCTTA AACAAGGAGG CACACTGTCT
    CTAATAGAGT GTACTCTTAT TGAGGAACTG GACACCAGTG ATGAAGACTA TAATGCCGCT
    GGTCAGGGTC TCAATCATCT CGAGTTTAAG ATCGTGGTTG AACCTGCTGA TGGTTCCTCG
    TTTTCAGTGG TTCTTCTCGC TCCATCTCGA CAGGAAAAAG CAGCCTGGAC CAGCGACATC
    AGCCAGTGCA TTGACAACAT TCGCTGCAAC GGCTTGATGA ACAGCGTGTT TGAGGACAAC
    TCGAAAGTCA CCGTGCCTCA CATGATCAAG TCTGACGCCC GACTGCACAA AGACGATGTG
    GACATCTGCT TCAGCAAGAC GCTCAACTCC TGCAAGGTGC CACAGATCCG CTACGCCAGC
    GTGGAGAGAC TGCTGGAAAG ACTGACCGAC CTGCGCTTTC TGTCCATCGA CTTCCTCAAC
    ACTTTCCTCC ACACATACCG CATCTTCACC ACTGCCGCAG TAGTGATGGA CAAACTGGCC
    GACATCTACA AGAAACCCTT CACGTCTATA CCTGTCAGGT CCCTGGAGCT TTTCTTCGCC
    ACCAACCAGG GCCCCTGGAG CGGAGAGCAT CTCAACAACA AATCCCCCCG GCTGTCCCGC
    AAGTTCTCCT CACCTCCGCC CATCTCCATC ACCTCGCGCA CCTCCTCCCC CATCCACTCC
    CGCAAGCTGT CCCTCAGCTC TCCTGTCAGC TGTAAGGCTG GAGCTCTGGA TCTCTCCACC
    TCCACCTTCT CTGCAGCCAG CAGTCCAACA TCTCCATATA ACCCCATCAT CTGCTCTCCT
    CCGCCCACCT GCACCAAAGC CCCTCTGGAC CTCAGCCGGG GCCCCAGCTC CCCGGAGCTC
    TGCCCCTCCA CCCCGGAGGA AGGTGGAGAA CTGCCCCGCA TAGATGCTTT CTGTGGGAAA
    CTGCGGCGGA GTATTCGGCG AGCTGTACTT GAGTCAGTAT CACTGGACAA GTTCATTCCA
    GAGACACCCA CATCCAGTGA CTCTGGAGAA ATGTCTCCAA GCCGTTCCCC ATCCACCCCG
    AGACACCTTC GCTACCGACA GCCCGCAGGT CAGTGTCCTG ATAACTCCCG CTGTGCCGTG
    TCCCCCGCGT CCGCCTTCGC CATTGCCACC GCCGCAGCCG GACACAGCAG CCCGCAAGGA
    TTTACCAACC CAGAGAAATC CTACGACAAA GAGTTCCTCA TCCGCAGGGC GGCCACCAAC
    AGAGTGCTCA ACGTCCTGCG CCACTGGGTT TCTAAACACT CGCAGGACTT TGAGATGAAC
    GCAGAGCTGA CGACTGCTGT GATGGCTCTG CTGGAGGAGG TGCTGAGAGA TCCAGATCTT
    CTGCCGCAGG AGAGAAAAGC TGCAGTCAAT ATACTGAGTG CGCTTTCTCA GGAAGAACAA
    GACGATTCAC AATTGAAGAT TGAGGATATC GTCCAAATGG TGGACTGTCC GAGGGCCGAA
    TGCTTTGAGT CACTATCTGC AATGGAGCTA GCAGAACAAA TCACCCTGCT GGACCATATT
    GTGTTCCGGA ATATTCCCTA TGAGGAGTTT CTTGGTCAAG GCTGGATGAA GATGGACAAA
    AATGAAAGAA CTCCATACAT TATGAAGACC AGTCAGCACT TCAATGATAT GAGTAATCTG
    GTAGCTTCTG AGATTATGGC TCATGCAGAT GTGAGCTCCA GGGCTGGATC CATTGACAAG
    TGGCTGGCAG TGGCTGATAT CTGCCGCTGT CTCAACAACT ACAACGGAGT CTTGGAAATC
    ACATCTGCAC TGAACCGCAG CGCCATCTAC AGGCTGAAGA AGACATGGGC CAAGGTTTGC
    AAACAGACCA AGGCATTAAT GGATAGGCTG CAGAAGATCG TGTCGTCTGA AGGGAGGTTT
    AAGAACCTGA GGGAAACTCT GAAGAACTGT AACCCACCGT GTGTGCCCTA TCTGGGCATG
    TACCTGACAG ATTTGGCATT TATCGAGGAA GGAACACCCA ATTTCACGGA GGAGGGTCTC
    GTCAACTTCT CTAAAATGAG GATGATTTCA CACATAATAC GAGAGATTCG ACAGTTTCAG
    CAGGCGCCCT ATAGGATTGA ACTGCAACCT AAGGTGACAC AGTTCCTCCT GGATAAGACC
    CTTGTAATGG ACGAGGACAC GCTTTATGAA CTATCTCTGA AGATCGAGCC AAGACTTCCA
    CCTGCCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.