trioa cDNA ORF clone, Danio rerio(zebrafish)

The following trioa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trioa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa150092 XM_021468080.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150093 XM_021468081.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56062 XM_017352241.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56063 XM_017352242.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa56064 XM_017352243.2
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa150090 XM_021468078.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa150091 XM_021468079.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa42641 XM_009294420.2
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa39075 NM_001104526.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150087 XM_021468075.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150088 XM_021468076.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa150089 XM_021468077.1
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42636 XM_009294415.2
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42637 XM_009294416.2
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42638 XM_009294417.2
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42639 XM_009294418.2
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42640 XM_009294419.2
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa42642 XM_009294421.3
Latest version!
Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa56064 XM_017352243.1 Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa42642 XM_009294421.2 Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa150092
    Clone ID Related Accession (Same CDS sequence) XM_021468080.1
    Accession Version XM_021468080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGCA TTACGTTGAT TTGTGTCCTG TCTTTGTTTT ATCTCAGTTC
    CCATATCTCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323755.1
    CDS196..6033
    Translation

    Target ORF information:

    RefSeq Version XM_021468080.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGCA TTACGTTGAT TTGTGTCCTG TCTTTGTTTT ATCTCAGTTC
    CCATATCTCT CCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150093
    Clone ID Related Accession (Same CDS sequence) XM_021468081.1
    Accession Version XM_021468081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGTT CCCATATCTC TCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323756.1
    CDS196..5994
    Translation

    Target ORF information:

    RefSeq Version XM_021468081.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGTT CCCATATCTC TCCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56062
    Clone ID Related Accession (Same CDS sequence) XM_017352241.1
    Accession Version XM_017352241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGCATTA CGTTGATTTG TGTCCTGTCT TTGTTTTATC TCAGTTCCCA
    TATCTCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207730.1
    CDS193..5967
    Translation

    Target ORF information:

    RefSeq Version XM_017352241.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGCATTA CGTTGATTTG TGTCCTGTCT TTGTTTTATC TCAGTTCCCA
    TATCTCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56063
    Clone ID Related Accession (Same CDS sequence) XM_017352242.1
    Accession Version XM_017352242.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGTTCCC ATATCTCTCC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207731.1
    CDS193..5928
    Translation

    Target ORF information:

    RefSeq Version XM_017352242.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352242.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGTTCCC ATATCTCTCC ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56064
    Clone ID Related Accession (Same CDS sequence) XM_017352243.2 , XM_017352243.1
    Accession Version XM_017352243.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGCATTA CGTTGATTTG
    TGTCCTGTCT TTGTTTTATC TCAGTTCCCA TATCTCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207732.1
    CDS196..5940
    Translation

    Target ORF information:

    RefSeq Version XM_017352243.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGCATTA CGTTGATTTG
    TGTCCTGTCT TTGTTTTATC TCAGTTCCCA TATCTCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56064
    Clone ID Related Accession (Same CDS sequence) XM_017352243.2 , XM_017352243.1
    Accession Version XM_017352243.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGCATTA CGTTGATTTG
    TGTCCTGTCT TTGTTTTATC TCAGTTCCCA TATCTCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207732.1
    CDS193..5937
    Translation

    Target ORF information:

    RefSeq Version XM_017352243.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGCATTA CGTTGATTTG
    TGTCCTGTCT TTGTTTTATC TCAGTTCCCA TATCTCTCCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150090
    Clone ID Related Accession (Same CDS sequence) XM_021468078.1
    Accession Version XM_021468078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323753.1
    CDS194..7402
    Translation

    Target ORF information:

    RefSeq Version XM_021468078.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150091
    Clone ID Related Accession (Same CDS sequence) XM_021468079.1
    Accession Version XM_021468079.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323754.1
    CDS195..7148
    Translation

    Target ORF information:

    RefSeq Version XM_021468079.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42641
    Clone ID Related Accession (Same CDS sequence) XM_009294420.2
    Accession Version XM_009294420.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGACGTC TTCACGTCTT TCCGTCAGAC CCTCCAGTTC TGAGACCCCC
    AGCGCTGCAG AGCTCGTCAG TGCCATTGAG GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA
    GACAGACCCA GCACTCTGTC AGTGGAGCAG GGCGACAGCA GCAGCCCTTC ATTCAACCCA
    TCTGATAACT CTCTCCTGTC CTCCTCCTCT CCAATCGATG AGATAGAGGA ACGCAAGACC
    GGTTTCTTCA AGAAGCGCCA TTACGTCCTG CTGGAGCTGA TCGAGACGGA GAGAGACTAT
    GTGCGAGATC TCAGTTTGGT CGTGGAGGGC TACATGGCCA GGATGAGGGA GGACGGGGTT
    CCAGATGATA TGAAAGGAAA AGACAAGATC GTTTTTGGAA ACATTCAGCA GATATATGAC
    TGGCACAAAG ATTTCTTCCT GGGAGAGCTT GAGAAGTGCC TTGAGGACCC TGACCGGCTG
    GGCCCTCTCT TCCTCAAACA TGAGCGGAGA CTGCACATGT ACATTGTTTA CTGCCAGAAC
    AAACCTAAAT CTGAACACAT CGTCTCAGAG TACATCGACA CATATTTTGA GGATCTGAAG
    CAGCGTTTGG GTCACAGGCT TCAGATCACT GATCTGCTCA TTAAACCTGT ACAGAGAATC
    ATGAAGTACC AGCTGCTGCT GAAGGATTTC CTCAAATTTT CCAAAAAAAT CGGAACAGAT
    TCGATTGAAT TAGAGAAAGC GGTTGAGGTC ATGTGCATTG TACCAAAAAG ATGCAACGAC
    ATGATGAATG TTGGTCGACT CCAGGGGTTT GATGGAAAGA TTGTTGCTCA GGGGCGTCTC
    CTACTCCAGG ACACCTTCAT GGTGGCCGAA CCAGAGGGAG GCCTGCTGAA CAGGATGAAG
    GAGAGGAGAG TCTTCCTCTT TGAGCAGATC GTCATCTTCA GTGAACCTCT AGATAAAAAG
    AGGGGCTTCT CCATGCCTGG CTATTTGTAC AAATACAGCA TCAAGGTGAA CTGTCTGGGA
    CTTGAGGACA GTGTGGACGG AGACCCCTGT AAATTTGCAC TGACCTCCAG AACGTCCAAT
    AGCAGCAAAG ATGCCTTCAT TCTGCACTCG AGCCATCCTG GAGTACGACA AGTCTGGATG
    CTGCAGATCA GCCAAATCCT GGAGAGCCAA CGCAATTTCC TCAATGCACT TACATCTCCT
    ATAGACTACC AGAGGAACCA CGTGGAGGGT CCTGGTGTGT CAGGCAGCGG TGTACAGGCT
    GGAGGCAGCG GGGGGCAGAT GATGGCCCCT GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG
    GGTTCCCGTT CTCGTCCGTC CCGTATCCCC CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT
    CATCACTCTC CTGCCCTGGG GCCTGGAGCC CACGAGCATG ACGGCCCCGA CAAACTATCA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292695.1
    CDS192..7337
    Translation

    Target ORF information:

    RefSeq Version XM_009294420.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294420.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGACGTC TTCACGTCTT TCCGTCAGAC CCTCCAGTTC TGAGACCCCC
    AGCGCTGCAG AGCTCGTCAG TGCCATTGAG GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA
    GACAGACCCA GCACTCTGTC AGTGGAGCAG GGCGACAGCA GCAGCCCTTC ATTCAACCCA
    TCTGATAACT CTCTCCTGTC CTCCTCCTCT CCAATCGATG AGATAGAGGA ACGCAAGACC
    GGTTTCTTCA AGAAGCGCCA TTACGTCCTG CTGGAGCTGA TCGAGACGGA GAGAGACTAT
    GTGCGAGATC TCAGTTTGGT CGTGGAGGGC TACATGGCCA GGATGAGGGA GGACGGGGTT
    CCAGATGATA TGAAAGGAAA AGACAAGATC GTTTTTGGAA ACATTCAGCA GATATATGAC
    TGGCACAAAG ATTTCTTCCT GGGAGAGCTT GAGAAGTGCC TTGAGGACCC TGACCGGCTG
    GGCCCTCTCT TCCTCAAACA TGAGCGGAGA CTGCACATGT ACATTGTTTA CTGCCAGAAC
    AAACCTAAAT CTGAACACAT CGTCTCAGAG TACATCGACA CATATTTTGA GGATCTGAAG
    CAGCGTTTGG GTCACAGGCT TCAGATCACT GATCTGCTCA TTAAACCTGT ACAGAGAATC
    ATGAAGTACC AGCTGCTGCT GAAGGATTTC CTCAAATTTT CCAAAAAAAT CGGAACAGAT
    TCGATTGAAT TAGAGAAAGC GGTTGAGGTC ATGTGCATTG TACCAAAAAG ATGCAACGAC
    ATGATGAATG TTGGTCGACT CCAGGGGTTT GATGGAAAGA TTGTTGCTCA GGGGCGTCTC
    CTACTCCAGG ACACCTTCAT GGTGGCCGAA CCAGAGGGAG GCCTGCTGAA CAGGATGAAG
    GAGAGGAGAG TCTTCCTCTT TGAGCAGATC GTCATCTTCA GTGAACCTCT AGATAAAAAG
    AGGGGCTTCT CCATGCCTGG CTATTTGTAC AAATACAGCA TCAAGGTGAA CTGTCTGGGA
    CTTGAGGACA GTGTGGACGG AGACCCCTGT AAATTTGCAC TGACCTCCAG AACGTCCAAT
    AGCAGCAAAG ATGCCTTCAT TCTGCACTCG AGCCATCCTG GAGTACGACA AGTCTGGATG
    CTGCAGATCA GCCAAATCCT GGAGAGCCAA CGCAATTTCC TCAATGCACT TACATCTCCT
    ATAGACTACC AGAGGAACCA CGTGGAGGGT CCTGGTGTGT CAGGCAGCGG TGTACAGGCT
    GGAGGCAGCG GGGGGCAGAT GATGGCCCCT GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG
    GGTTCCCGTT CTCGTCCGTC CCGTATCCCC CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT
    CATCACTCTC CTGCCCTGGG GCCTGGAGCC CACGAGCATG ACGGCCCCGA CAAACTATCA
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39075
    Clone ID Related Accession (Same CDS sequence) NM_001104526.1
    Accession Version NM_001104526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX957244.15 and BX950864.17. On Sep 11, 2007 this sequence version replaced XM_680764.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA4476748 [ECO:0000350] ##Evidence-Data-END##

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    1021
    1081
    1141
    1201
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    1321
    1381
    1441
    1501
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    1621
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    1801
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    1981
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    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGTAAAGC CTTGACGCCG CTCTTGGGAT TTCGCAGGAA TGAAGAAATG
    AAGGCGATGG AGGTGTTGCC CATTTTAAAA GAAAAAGTGG CCTTCTTATC AGGGGGCAGA
    GACAAGCGTG GCGGTCCAGT TTTAACATTC CCATCACGAA CCAACCATGA CCGAATACGA
    CACGAGGATC TCCGCAGACT CATCGCCTAC CTCGCCGGCA TCCCAAGCGA GGATGTCTGC
    AAACATGGCT TCACGGTCAT CGTGGACATG CGTGGCTCCA AATGGGATTC CATCAAGCCT
    CTGCTGAAGA TCCTCCAGGA GTCGTTCCCC TGCTGCATCC ACATCGCCCT CATCATCAAA
    CCAGACAACT TCTGGCAGAA ACAGAGGACC AATTTCGGCA GCTCCAAGTT TGAGTTTGAG
    ACCACAATGG TTTCTTTGGA GGGCTTATCG AAGGTGGTGG ACCCATCCCA GTTGACGGCT
    GACTTTGAGG GCAGCCTGGA CTACAACCAT GAAGAGTGGA TTGAAATCCG CGTGGCCTTC
    GAAGACTTTA CAGGCAATGC TCGGCACCTG CTCGCCCGGC TAGAAGAGAT GCATGAAACG
    GTGACGCGTA AAGACTTACC GCAGGACTTA GATGGAGCCC GACGGATGAT AGAGGAGCAC
    GCGGCTTTGA AGAAGAGGGT GATAAAGGCT CCGGTGGAGG AGGTGGACAA TGAGGGCCAA
    AGGCTCCTGC AAAGGATCCA GAGCAGTGAG AGCTACGCCA ACCGAACTGT GCCTGTGCCG
    CCTGGTCAGA GGGAAGGGCA GGGGCAGCCC AACGCCGACA CCCAGGGCCT GGTGCCCCGT
    ATTACAGCTC TGCTGGAGAA ACTGCACAGC ACCAGGCAGA ACCTGCACCA GTCCTGGCAC
    ATCCGCAAGC TGCAGCTGGA CCAGTGCTTC CAGCTCAGGC TGTTCGAGCA GGACGCTGAG
    AAGATGTTTG ATTGGATCAT GCACAATAAA GGGCTGTTTC TGGCTGGATA CACTGAGATC
    GGCAACAACC ACCCTCACGC CATGGAGCTG CAGACCCAGC ACAATCACTT CGCCATGAAC
    TGCATGAATG TTTACGTGAA CATAAACCGC ATCATGTCTG TGGGGAACCG GCTGCTGGAG
    GCCGGTCATT ACGCGTCGCA GCAGATCAAG CAGATCTCAG GTCAGCTGGA GCAGGAGTGG
    AAAGCTTTCG CCGCTGCGCT GGACGAGAGG AGTGCTCTGC TGGAGATGTC TGCTACCTTC
    CACCAGAAAT GCGACCAGTA CATGAGTAAT GTGGACTCGT GGTGTAAAGC GTGTGGAGAA
    GTGGATCTGC CCTCAGAGCT GCAGGAACTG GAGGACGCTA TACACCATCA CCAGGGCCTG
    TATGAACACA TCACCGCTGC CTACTCGGAG GTGAGCCAAG ACGGAAAAGC CTTATTGGAC
    AAACTTCAGC GCCCCTTGAC GCCCGGCAGT GCTGATTCGC TGACAGCTTC CGCCAATTAC
    ACCAAGGCGG TGAACCATGT TTTGGACATC ATCCATGAGG TTCTGCATCA CCAAAGGCAG
    CTGGAGAACA TCTGGCAACA TCGCAAGGTC CGGCTGCACC AGCGTCTGCA GCTGTGTGTT
    TTCCAGCAGG ACGTTCAGCA GGTTCTGGAC TGGATTGAGA ACCATGGCGA GGCATTTCTC
    AGCAAGCACA CAGGGGTGGG AAAATCTCTG CATCGCGCTC GAGCTCTGCA GAAACGCCAT
    GAAGACTTTG AGGAAGTGGC TCAGAACACG TACACAAATG CGGATAAGCT TCTGGAAGCG
    GCAGAGCAGC TGGCCCAGAC AGGCGAGTGT GACCCAGAGG AGATCTATCA GGCCGCTCAT
    CAGCTGGAGG ACCGAATCCA GGACTTTGTT CGGCGCGTGG AGCAGCGCAA GGTCCTGCTG
    GACATGTCAG TGGCTTTCCA CACTCACGTC AAAGAGTTGT GGACGTGGCT GGAGGAGCTG
    CAGAAGGAGT TGCTGGACGA TGTGTACGCC GAGTCTGTGG AAGCCGTGCA GGATCTGATC
    AAGCGTTTCG GGCAGCAGCA ACAAACCACA CTGCAGTTCA CTGTAAACGT CATCAAAGAA
    GGAGAAGATC TCATCCAGCA GCTCAGAGAC CTGGCCATCT CCAGCAACAA GACGCCTCAC
    AACAGCTCCA TCAACCACAT CGAGAGCGTC CTGCAGCAGC TGGATGAGGC TCAGGCTCAG
    ATGGAGGAGC TTTTCCAGGA GAGGAAGATC AAGCTGGAGC TTTTCCTGCA GCTCAGGATC
    TTTGAGCGGG ACGCCATAGA TATTATCTCA GATCTGGACT CGTGGAATGA GGAGCTGTCG
    CAGCAGATGA ATGAGTTTGA TACAGAAGAC CTGACGCTGG CTGAACAGAG ACTGCAGCAC
    CATGCTGATA AAGCCCTGAC CATGAACAAC CTGGCCTTCC ACGTCATCCA CCAGGGCCAG
    GAACTGCTGC AGTACGTCAA CGAGGTGCAG GCGTCTGGTG TGGAGTTGCT GTGTGACCGT
    GATGTGGACA TGGCCACCCG TGTTCAGGAC CTGCTAGAGT TTCTACATGA GAAACAGCAA
    GAGCTGGACG TGGCTGCAGA ACAGCATCGC AGACACCTTG AGCAGTGTGT GCAACTGCGC
    CACCTACAAG CCGAAGTCAA ACAGGTTCTG GGTTGGATCC GTAATGGCGA GTCGATGCTG
    AATGCAGGTC TGATTACGGC CAGCTCTCTT CAAGAAGCAG AGCAGTTGCA GAGAGAGCAT
    GAGCAATTCC AGCATGCTAT CGAGAAAACC CATCAGAGCG CCCTGCAGGT CCAGCAGAAA
    GCCGAAGCCC TGCTGCAGGC CAATCACTAC GACATGGACA TGATTCGAGA CTGCGCGGAG
    AACGTGGCTT CCCACTGGCA GCAGCTCATG CTGAAGATGG AGGACCGGCT CAAGCTGGTC
    AACGCCTCCG TGGCCTTCTA TAAGACCTCT GAACAGGTTT GCAGTGTTCT GGAGAGTCTG
    GAGCAGGAGT ACAAGCGTGA GGAGGACTGG TGCGGAGGAG CTGACAAACT GGGACCCAAC
    TGTGAATCGG ACCACGTCAC ACCCATGATC AGCAAACACC TGGAGCAGAA AGAGGCTTTC
    CTCAAGGCCT GCACGTTGGC CAGGCGTAAT GCAGACGTCT TCCTCAAATA CATGCACAGG
    AACAGCGTCA GCATGCCCGG GATGTTGAGT CACGTCAAAG CTCCAGAGCA GCAGGTCAAA
    AATATCCTGA ATGAGCTGCT GCAGAGGGAG AACCGCGTGC TGCACTTCTG GACTATGAGA
    AAGAGGAGGC TGGACCAGTG TCAGCAATAC GTGGTGTTTG AACGCAGCGC CAAACAGGCT
    CTGGAGTGGA TCCACGACAC TGGCGAGTTT TATCTGTCCA CACACACGTC CACCGGCTCC
    AGCATACACC ACACACAGGA GCTGCTGAAG GAGCACGAGG ACTTCCACAT CACAGCCAAG
    CAAACCAAAG AACGGGTGAA GCTGCTGATC CAGCTGGCAG ATGGTTTCTG TGATAAGGGT
    CACTCTCACG CCGCTGAGAT TAAGAAATGG GTGACAGCGG TGGACAAACG CTATCGAGAT
    TTCTCGCTCC GCATGGACAA GTACCGCACA TCTCTGGAGA AAGCACTCGG CATCTCCTCA
    GACTCCAATA AAGCTAGCAA AGACTTGCAG CTGGACATCA TCCCTGCCAG TGCTCCGGGG
    TCAGAGGTCA AACTCAGAGA CGCTGCTCAT GAGCTCAATG AAGAGAAGCG CAAGTCTGCC
    CGCAGGAAGG ACTTCATCAT GGCTGAGCTG ATCCAGACAG AAAAGGCCTA TGTGCGAGAC
    TTGAGAGAGT GCATGGACAC GTATCTGTGG GAGATGACCA GCGGCGTGGA GGAGATCCCA
    CCAGGCATCG TCAACAAAGA GCACATCATT TTTGGTAACA TGCAAGATCT GTACGAGTTC
    CATCACAATA TATTCCTCAA AGAGCTGGAG AAATACGAGC AGCTTCCTGA AGATGTGGGC
    CACTGTTTTG TGACTTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG CAAGAACAAA
    CCAGACTCCA CTCAGCTTAT TCTTGAACAT GCCGGAGGAT ACTTCGACGA GATCCAGCAG
    AGGCATCGCC TGGCCAACTC CATCTCCTCA TACCTCATCA AACCTGTTCA GAGAATAACC
    AAGTACCAGC TGCTCCTCAA GGAGCTCCTG ACGTGCTGCG AGGAAGGCAA AGGTGAAATT
    AAAGATGGCC TGGAGGTGAT GCTCAGTGTT CCCAAAAGAG CCAACGACGC CATGCACTTG
    AGCATGCTTG AAGGGTTTGA TGAGAACATC GAGTCCCAGG GAGAGCTGAT CCTGCAGGAG
    GCGTTTCAGG TGTGGGATCC CAAAACTCTG ATCCGCAAGG GAAGAGAACG ACACCTGTTC
    CTCTTCGAGA TGTCACTGAT CTTCAGCAAG GAGGTCAAAG ACTCCAATGG CAGGAGCAAG
    TACATCTACA AGAGCAAGCT TTTCACCTCT GAACTGGGTG TCACTGAGCA TGTGGAGGGA
    GATCCATGCA AATTTGCTCT CTGGGTTGGA CGAACCCCAA CTTCTGACAA CAAGATTGTC
    CTCAAGGCGT CCGGTATTGA GAACAAACAG GACTGGATCA AGCACATCCG GGAAGTGATT
    CAGGAGCGCA CCGTTCACCT GAAGGGGGCG CTGAAAGAGC CCATTCACAT CCCCAAAGCC
    TCCACAGCCA AACACAAGGG GCGCAGGGAT GGAGAGGATC TGGACAGTCA GGGTGACGGC
    AGCAGTCAAC CAGACACCAT CTCACTGGCC TCCAGGACCT CACAGAACAC ACTGGACAGC
    GACAAGTTGT CTGGTGGCTG TGAGCTGACG GTGGTCATCC ATGACTTTAT GGCCGGTAAC
    AGTAATGAGC TGACCATCCG GAGAGGACAG ACGGTGGAGG TGCTCGAGAG ACTTCATGAT
    AAGCCGGACT GGTGTTTGGT TCGCACCACA GACCGTTCGC CGGCGCTGGA GGGCCTGGTG
    CCCTGTGCCA TGCTCTGTAT CGCCCACTCC CGCAGCAGCA TGGAGATGGA GGGCATCTTC
    AACCACAAAG ATGCACTCTC TGTATGCAGT AATGATGCCA TCATGCCAGG CTCCTCTGCC
    ACCCTGCAGC CGGGTCATGT GATGGGTTCC CAGAGTTCTC CTGGACCTAA AAGGCCTGGC
    AACACATTAA GGAAATGGCT GACCAGTCCT GTGAGGAGAC TGAGCAGCGG TAAAGCTGAC
    GGACACGTCA AGAAGCTGGC ACACAAACAC AAGAAGAGCC GTGACGTCAG AAAGAGCGCA
    GACGCCGGTT CACAGAAAGA CTCGGACGAC AGCGCTGCGA CGCCACAAGA TGAGACCCTC
    GAGGAGAGGG TGCGTAATGA GGGTCTGAGC AGCGGCACGC TCTCCAAATC CTCCTCTTCA
    GGGATGCAGA GCTGTGGAGA GGAAGAGGGA GAGGAAGGAG CTGACGCTGT GCCTCTTCCT
    CCACCAATGG CCATCCAGCA GCACAGCCTC CTCCAGCCGG ATGCGCAGGA TGACAAGACG
    TCTTCACGTC TTTCCGTCAG ACCCTCCAGT TCTGAGACCC CCAGCGCTGC AGAGCTCGTC
    AGTGCCATTG AGGAGCTGGT CAAAAGCAAG ATGGCTCTGG AAGACAGACC CAGCACTCTG
    TCAGTGGAGC AGGGCGACAG CAGCAGCCCT TCATTCAACC CATCTGATAA CTCTCTCCTG
    TCCTCCTCCT CTCCAATCGA TGAGATAGAG GAACGCAAGA CCGGTTTCTT CAAGAAGCGC
    CATTACGTCC TGCTGGAGCT GATCGAGACG GAGAGAGACT ATGTGCGAGA TCTCAGTTTG
    GTCGTGGAGG GCTACATGGC CAGGATGAGG GAGGACGGGG TTCCAGATGA TATGAAAGGA
    AAAGACAAGA TCGTTTTTGG AAACATTCAG CAGATATATG ACTGGCACAA AGATTTCTTC
    CTGGGAGAGC TTGAGAAGTG CCTTGAGGAC CCTGACCGGC TGGGCCCTCT CTTCCTCAAA
    CATGAGCGGA GACTGCACAT GTACATTGTT TACTGCCAGA ACAAACCTAA ATCTGAACAC
    ATCGTCTCAG AGTACATCGA CACATATTTT GAGGATCTGA AGCAGCGTTT GGGTCACAGG
    CTTCAGATCA CTGATCTGCT CATTAAACCT GTACAGAGAA TCATGAAGTA CCAGCTGCTG
    CTGAAGGATT TCCTCAAATT TTCCAAAAAA ATCGGAACAG ATTCGATTGA ATTAGAGAAA
    GCGGTTGAGG TCATGTGCAT TGTACCAAAA AGATGCAACG ACATGATGAA TGTTGGTCGA
    CTCCAGGGGT TTGATGGAAA GATTGTTGCT CAGGGGCGTC TCCTACTCCA GGACACCTTC
    ATGGTGGCCG AACCAGAGGG AGGCCTGCTG AACAGGATGA AGGAGAGGAG AGTCTTCCTC
    TTTGAGCAGA TCGTCATCTT CAGTGAACCT CTAGATAAAA AGAGGGGCTT CTCCATGCCT
    GGCTATTTGT ACAAATACAG CATCAAGGTG AACTGTCTGG GACTTGAGGA CAGTGTGGAC
    GGAGACCCCT GTAAATTTGC ACTGACCTCC AGAACGTCCA ATAGCAGCAA AGATGCCTTC
    ATTCTGCACT CGAGCCATCC TGGAGTACGA CAAGTCTGGA TGCTGCAGAT CAGCCAAATC
    CTGGAGAGCC AACGCAATTT CCTCAATGCA CTTACATCTC CTATAGACTA CCAGAGGAAC
    CACGTGGAGG GTCCTGGTGT GTCAGGCAGC GGTGTACAGG CTGGAGGCAG CGGGGGGCAG
    ATGATGGCCC CTGGAGGAGG AGTAGGGGTC CCGGCTGGGC CGGGTTCCCG TTCTCGTCCG
    TCCCGTATCC CCCAGCCCTC GCGCTTGCCT CAACCGCTCC GTCATCACTC TCCTGCCCTG
    GGGCCTGGAG CCCACGAGCA TGACGGCCCC GACAAACTAT CAGGTATGTC CCCAAGACCT
    CTCTCCAGGG GTCCCTCACC CTCATGCACC ACAGAGCCCG AGCCAAAGGT GAAGCTTCCT
    GCGAGCCCTC ACCCCAAACA GACGGACTCC CAACAGACAG AAAGCCCAGC CAAAGAGATC
    CCACGAGCCA CTGTGGCCCC GCTGGCTCTT GTCAAACCCA GACCTGGAAC GGTTTCACCA
    ATGGCCTCTC CGTTAGCCAC ACCGGCTTTC AAAGACTCCA TTCCACCCTG TAGTCCAGGC
    CCGAAGACTG GCAGCAGCTC GTTTTGGAGC TCAGTGCCCG CGTCCCCCGC CAGCAGGCCT
    GCTTCCTTCA CCTTCCCCGG GGACGCCTGC GACACGCTGA GCCGACCAAA TCACAACCAG
    AGTCAGCGGC ACTCCACCCA CAGTAAAGAC GCAGACCGTA TGAGCACCTG CTCCTCTACC
    AGTGAGCAGT CTATCCAGTC CACTCAGAGC AATGGGAGTG AAAGCAGCAG CAGCAGTAAT
    ATCTCCACCA TGTTGGTGAC TCAGGACTAT GTGGCTCTGA AAGAGGATGA GATTAATGTG
    GGTCAGGGGG AGGTGGTGCA GATCCTGGCC TCCAATCAGC AGAATATGTT CTTGGTGTTT
    CGTGCCGCTA CTGAGCAGTG TCCTGCCGCC GAGGGCTGGA TCCCCGGATA CGTGCTGGGA
    CACACATCCG CCATCATCCC TGACGCTCCA GACGGAACCA TAAAGAAATC GTCATCATCA
    TGGCACACAT CCCTCCGTAT TCGAAAGAAG TCAGAGAAAA GAGAAAAGGA CAATAAAAAA
    GAGGCCAAGC TGGAAAACGG CTACCGGAAA TCCAGAGAGG GACTGACCAA CAAGGTCTCT
    GTGAAGCTTC TAAATCCCAA TTATATATAT GATGTTCTCC CGGAGTTCCT CGTCCCGCTG
    AGTGACGTGA CCTGTGATAA AGGCGAGTGT GTGACTCTGA GGTGTAAAGT GTGTGGTCGG
    CCCAAAGCCA CGGTCACGTG GAAGGGACCT GAGCAGAAAA CACTCACCAA CAACGGCCAC
    TTCAGCATCG CCTACAGTGA GACTGGTGAG GCCACGCTGC GGATCGTAGG TGTGACCTCA
    GAGGATGATG GCAGTTACAC CTGCATTGCC ACGAATGACA TTGGCAGTGT GGCATCATCA
    GCCAGTCTCA GAGTGCTGGG TAAGAACCAC GATGGGATCC GGGTCATGTG GAAAGACAAC
    TTTGAGTCCT TCTACACTGA GGTAGCAGAG CTTGGCAGGG GACGGTTCTC TGTAGTGAAA
    CGCTGTGACC AGAGGGGATC AAAGCGGACC GTGGCGGTCA AGTTTGTCAA TAAGAAACTG
    ATGAAGCGAG ACCAGGTGAC CCATGAGTTC AGTGTCCTAC AGAGACTCCA GCACCCTCAT
    CTGGTCAGAC TGCTGGACAC CTTCGAGACC TCCAGCAGCT ACGCACTGGT CCTGGAGATG
    TCTGATCAGG GCCGTCTGCT GGACTACATC GTGAGCTGGG GGAATCTCAC TGAGGAGAAA
    GTGGCCTTTT ACCTCCGGGA CATTTTAGAA GCTTTGCAGT ACCTGCACAA CTGCAGAATA
    GTCCATCTGG ATCTAAAGCC TGAGAATCTG GTGGTGGAGC AGAGCCCGTC TCAGCCGCTG
    GTCAAGCTGA CGGACTTCGG TGATGCTGTG CAGCTCAACT CCACGCCGTA CGTTCACCCT
    CTGCTGGGGA GCCCGGAGTT CGCAGCCCCT GAGCTGGTCC TCGGGGACCC TGTCTCTCTG
    TCCTCCGACC TGTGGAGTCT GGGGGTCCTG ACGTACGTCA TGCTGAGCGG AGCATCTCCG
    TTCCTTGATG AGAGCGTGGA GGAGACCTGC CTCAACATCT GCCGCTTGGA CTTCAGCTTT
    CCTGATGACT ACTTCCAGGG AGTGAGTCAG GCGGCGCGTG ATTTTATGTG CCTGCTGCTC
    CGGATGGAGC CATCCAAACG TCCACCAGCC ACCTCATGTC TACAGGAGCC CTGGTTGAGA
    GCCGGCGGGG GCCGACGCTC AGCCGAATGC ATCGATACCT CCAGACTGAT CTCCTTCATC
    GACCGACGCA AACACCAGAA CGACCTGCGG CCTCTCACTG GCATCAGAGC CTTCCTGCAG
    ACTCGATTAC AGCCCAGGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097996.1
    CDS1..9264
    Translation

    Target ORF information:

    RefSeq Version NM_001104526.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001104526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGTAAAGC CTTGACGCCG CTCTTGGGAT TTCGCAGGAA TGAAGAAATG
    AAGGCGATGG AGGTGTTGCC CATTTTAAAA GAAAAAGTGG CCTTCTTATC AGGGGGCAGA
    GACAAGCGTG GCGGTCCAGT TTTAACATTC CCATCACGAA CCAACCATGA CCGAATACGA
    CACGAGGATC TCCGCAGACT CATCGCCTAC CTCGCCGGCA TCCCAAGCGA GGATGTCTGC
    AAACATGGCT TCACGGTCAT CGTGGACATG CGTGGCTCCA AATGGGATTC CATCAAGCCT
    CTGCTGAAGA TCCTCCAGGA GTCGTTCCCC TGCTGCATCC ACATCGCCCT CATCATCAAA
    CCAGACAACT TCTGGCAGAA ACAGAGGACC AATTTCGGCA GCTCCAAGTT TGAGTTTGAG
    ACCACAATGG TTTCTTTGGA GGGCTTATCG AAGGTGGTGG ACCCATCCCA GTTGACGGCT
    GACTTTGAGG GCAGCCTGGA CTACAACCAT GAAGAGTGGA TTGAAATCCG CGTGGCCTTC
    GAAGACTTTA CAGGCAATGC TCGGCACCTG CTCGCCCGGC TAGAAGAGAT GCATGAAACG
    GTGACGCGTA AAGACTTACC GCAGGACTTA GATGGAGCCC GACGGATGAT AGAGGAGCAC
    GCGGCTTTGA AGAAGAGGGT GATAAAGGCT CCGGTGGAGG AGGTGGACAA TGAGGGCCAA
    AGGCTCCTGC AAAGGATCCA GAGCAGTGAG AGCTACGCCA ACCGAACTGT GCCTGTGCCG
    CCTGGTCAGA GGGAAGGGCA GGGGCAGCCC AACGCCGACA CCCAGGGCCT GGTGCCCCGT
    ATTACAGCTC TGCTGGAGAA ACTGCACAGC ACCAGGCAGA ACCTGCACCA GTCCTGGCAC
    ATCCGCAAGC TGCAGCTGGA CCAGTGCTTC CAGCTCAGGC TGTTCGAGCA GGACGCTGAG
    AAGATGTTTG ATTGGATCAT GCACAATAAA GGGCTGTTTC TGGCTGGATA CACTGAGATC
    GGCAACAACC ACCCTCACGC CATGGAGCTG CAGACCCAGC ACAATCACTT CGCCATGAAC
    TGCATGAATG TTTACGTGAA CATAAACCGC ATCATGTCTG TGGGGAACCG GCTGCTGGAG
    GCCGGTCATT ACGCGTCGCA GCAGATCAAG CAGATCTCAG GTCAGCTGGA GCAGGAGTGG
    AAAGCTTTCG CCGCTGCGCT GGACGAGAGG AGTGCTCTGC TGGAGATGTC TGCTACCTTC
    CACCAGAAAT GCGACCAGTA CATGAGTAAT GTGGACTCGT GGTGTAAAGC GTGTGGAGAA
    GTGGATCTGC CCTCAGAGCT GCAGGAACTG GAGGACGCTA TACACCATCA CCAGGGCCTG
    TATGAACACA TCACCGCTGC CTACTCGGAG GTGAGCCAAG ACGGAAAAGC CTTATTGGAC
    AAACTTCAGC GCCCCTTGAC GCCCGGCAGT GCTGATTCGC TGACAGCTTC CGCCAATTAC
    ACCAAGGCGG TGAACCATGT TTTGGACATC ATCCATGAGG TTCTGCATCA CCAAAGGCAG
    CTGGAGAACA TCTGGCAACA TCGCAAGGTC CGGCTGCACC AGCGTCTGCA GCTGTGTGTT
    TTCCAGCAGG ACGTTCAGCA GGTTCTGGAC TGGATTGAGA ACCATGGCGA GGCATTTCTC
    AGCAAGCACA CAGGGGTGGG AAAATCTCTG CATCGCGCTC GAGCTCTGCA GAAACGCCAT
    GAAGACTTTG AGGAAGTGGC TCAGAACACG TACACAAATG CGGATAAGCT TCTGGAAGCG
    GCAGAGCAGC TGGCCCAGAC AGGCGAGTGT GACCCAGAGG AGATCTATCA GGCCGCTCAT
    CAGCTGGAGG ACCGAATCCA GGACTTTGTT CGGCGCGTGG AGCAGCGCAA GGTCCTGCTG
    GACATGTCAG TGGCTTTCCA CACTCACGTC AAAGAGTTGT GGACGTGGCT GGAGGAGCTG
    CAGAAGGAGT TGCTGGACGA TGTGTACGCC GAGTCTGTGG AAGCCGTGCA GGATCTGATC
    AAGCGTTTCG GGCAGCAGCA ACAAACCACA CTGCAGTTCA CTGTAAACGT CATCAAAGAA
    GGAGAAGATC TCATCCAGCA GCTCAGAGAC CTGGCCATCT CCAGCAACAA GACGCCTCAC
    AACAGCTCCA TCAACCACAT CGAGAGCGTC CTGCAGCAGC TGGATGAGGC TCAGGCTCAG
    ATGGAGGAGC TTTTCCAGGA GAGGAAGATC AAGCTGGAGC TTTTCCTGCA GCTCAGGATC
    TTTGAGCGGG ACGCCATAGA TATTATCTCA GATCTGGACT CGTGGAATGA GGAGCTGTCG
    CAGCAGATGA ATGAGTTTGA TACAGAAGAC CTGACGCTGG CTGAACAGAG ACTGCAGCAC
    CATGCTGATA AAGCCCTGAC CATGAACAAC CTGGCCTTCC ACGTCATCCA CCAGGGCCAG
    GAACTGCTGC AGTACGTCAA CGAGGTGCAG GCGTCTGGTG TGGAGTTGCT GTGTGACCGT
    GATGTGGACA TGGCCACCCG TGTTCAGGAC CTGCTAGAGT TTCTACATGA GAAACAGCAA
    GAGCTGGACG TGGCTGCAGA ACAGCATCGC AGACACCTTG AGCAGTGTGT GCAACTGCGC
    CACCTACAAG CCGAAGTCAA ACAGGTTCTG GGTTGGATCC GTAATGGCGA GTCGATGCTG
    AATGCAGGTC TGATTACGGC CAGCTCTCTT CAAGAAGCAG AGCAGTTGCA GAGAGAGCAT
    GAGCAATTCC AGCATGCTAT CGAGAAAACC CATCAGAGCG CCCTGCAGGT CCAGCAGAAA
    GCCGAAGCCC TGCTGCAGGC CAATCACTAC GACATGGACA TGATTCGAGA CTGCGCGGAG
    AACGTGGCTT CCCACTGGCA GCAGCTCATG CTGAAGATGG AGGACCGGCT CAAGCTGGTC
    AACGCCTCCG TGGCCTTCTA TAAGACCTCT GAACAGGTTT GCAGTGTTCT GGAGAGTCTG
    GAGCAGGAGT ACAAGCGTGA GGAGGACTGG TGCGGAGGAG CTGACAAACT GGGACCCAAC
    TGTGAATCGG ACCACGTCAC ACCCATGATC AGCAAACACC TGGAGCAGAA AGAGGCTTTC
    CTCAAGGCCT GCACGTTGGC CAGGCGTAAT GCAGACGTCT TCCTCAAATA CATGCACAGG
    AACAGCGTCA GCATGCCCGG GATGTTGAGT CACGTCAAAG CTCCAGAGCA GCAGGTCAAA
    AATATCCTGA ATGAGCTGCT GCAGAGGGAG AACCGCGTGC TGCACTTCTG GACTATGAGA
    AAGAGGAGGC TGGACCAGTG TCAGCAATAC GTGGTGTTTG AACGCAGCGC CAAACAGGCT
    CTGGAGTGGA TCCACGACAC TGGCGAGTTT TATCTGTCCA CACACACGTC CACCGGCTCC
    AGCATACACC ACACACAGGA GCTGCTGAAG GAGCACGAGG ACTTCCACAT CACAGCCAAG
    CAAACCAAAG AACGGGTGAA GCTGCTGATC CAGCTGGCAG ATGGTTTCTG TGATAAGGGT
    CACTCTCACG CCGCTGAGAT TAAGAAATGG GTGACAGCGG TGGACAAACG CTATCGAGAT
    TTCTCGCTCC GCATGGACAA GTACCGCACA TCTCTGGAGA AAGCACTCGG CATCTCCTCA
    GACTCCAATA AAGCTAGCAA AGACTTGCAG CTGGACATCA TCCCTGCCAG TGCTCCGGGG
    TCAGAGGTCA AACTCAGAGA CGCTGCTCAT GAGCTCAATG AAGAGAAGCG CAAGTCTGCC
    CGCAGGAAGG ACTTCATCAT GGCTGAGCTG ATCCAGACAG AAAAGGCCTA TGTGCGAGAC
    TTGAGAGAGT GCATGGACAC GTATCTGTGG GAGATGACCA GCGGCGTGGA GGAGATCCCA
    CCAGGCATCG TCAACAAAGA GCACATCATT TTTGGTAACA TGCAAGATCT GTACGAGTTC
    CATCACAATA TATTCCTCAA AGAGCTGGAG AAATACGAGC AGCTTCCTGA AGATGTGGGC
    CACTGTTTTG TGACTTGGGC TGATAAGTTC CAGATGTATG TGAACTACTG CAAGAACAAA
    CCAGACTCCA CTCAGCTTAT TCTTGAACAT GCCGGAGGAT ACTTCGACGA GATCCAGCAG
    AGGCATCGCC TGGCCAACTC CATCTCCTCA TACCTCATCA AACCTGTTCA GAGAATAACC
    AAGTACCAGC TGCTCCTCAA GGAGCTCCTG ACGTGCTGCG AGGAAGGCAA AGGTGAAATT
    AAAGATGGCC TGGAGGTGAT GCTCAGTGTT CCCAAAAGAG CCAACGACGC CATGCACTTG
    AGCATGCTTG AAGGGTTTGA TGAGAACATC GAGTCCCAGG GAGAGCTGAT CCTGCAGGAG
    GCGTTTCAGG TGTGGGATCC CAAAACTCTG ATCCGCAAGG GAAGAGAACG ACACCTGTTC
    CTCTTCGAGA TGTCACTGAT CTTCAGCAAG GAGGTCAAAG ACTCCAATGG CAGGAGCAAG
    TACATCTACA AGAGCAAGCT TTTCACCTCT GAACTGGGTG TCACTGAGCA TGTGGAGGGA
    GATCCATGCA AATTTGCTCT CTGGGTTGGA CGAACCCCAA CTTCTGACAA CAAGATTGTC
    CTCAAGGCGT CCGGTATTGA GAACAAACAG GACTGGATCA AGCACATCCG GGAAGTGATT
    CAGGAGCGCA CCGTTCACCT GAAGGGGGCG CTGAAAGAGC CCATTCACAT CCCCAAAGCC
    TCCACAGCCA AACACAAGGG GCGCAGGGAT GGAGAGGATC TGGACAGTCA GGGTGACGGC
    AGCAGTCAAC CAGACACCAT CTCACTGGCC TCCAGGACCT CACAGAACAC ACTGGACAGC
    GACAAGTTGT CTGGTGGCTG TGAGCTGACG GTGGTCATCC ATGACTTTAT GGCCGGTAAC
    AGTAATGAGC TGACCATCCG GAGAGGACAG ACGGTGGAGG TGCTCGAGAG ACTTCATGAT
    AAGCCGGACT GGTGTTTGGT TCGCACCACA GACCGTTCGC CGGCGCTGGA GGGCCTGGTG
    CCCTGTGCCA TGCTCTGTAT CGCCCACTCC CGCAGCAGCA TGGAGATGGA GGGCATCTTC
    AACCACAAAG ATGCACTCTC TGTATGCAGT AATGATGCCA TCATGCCAGG CTCCTCTGCC
    ACCCTGCAGC CGGGTCATGT GATGGGTTCC CAGAGTTCTC CTGGACCTAA AAGGCCTGGC
    AACACATTAA GGAAATGGCT GACCAGTCCT GTGAGGAGAC TGAGCAGCGG TAAAGCTGAC
    GGACACGTCA AGAAGCTGGC ACACAAACAC AAGAAGAGCC GTGACGTCAG AAAGAGCGCA
    GACGCCGGTT CACAGAAAGA CTCGGACGAC AGCGCTGCGA CGCCACAAGA TGAGACCCTC
    GAGGAGAGGG TGCGTAATGA GGGTCTGAGC AGCGGCACGC TCTCCAAATC CTCCTCTTCA
    GGGATGCAGA GCTGTGGAGA GGAAGAGGGA GAGGAAGGAG CTGACGCTGT GCCTCTTCCT
    CCACCAATGG CCATCCAGCA GCACAGCCTC CTCCAGCCGG ATGCGCAGGA TGACAAGACG
    TCTTCACGTC TTTCCGTCAG ACCCTCCAGT TCTGAGACCC CCAGCGCTGC AGAGCTCGTC
    AGTGCCATTG AGGAGCTGGT CAAAAGCAAG ATGGCTCTGG AAGACAGACC CAGCACTCTG
    TCAGTGGAGC AGGGCGACAG CAGCAGCCCT TCATTCAACC CATCTGATAA CTCTCTCCTG
    TCCTCCTCCT CTCCAATCGA TGAGATAGAG GAACGCAAGA CCGGTTTCTT CAAGAAGCGC
    CATTACGTCC TGCTGGAGCT GATCGAGACG GAGAGAGACT ATGTGCGAGA TCTCAGTTTG
    GTCGTGGAGG GCTACATGGC CAGGATGAGG GAGGACGGGG TTCCAGATGA TATGAAAGGA
    AAAGACAAGA TCGTTTTTGG AAACATTCAG CAGATATATG ACTGGCACAA AGATTTCTTC
    CTGGGAGAGC TTGAGAAGTG CCTTGAGGAC CCTGACCGGC TGGGCCCTCT CTTCCTCAAA
    CATGAGCGGA GACTGCACAT GTACATTGTT TACTGCCAGA ACAAACCTAA ATCTGAACAC
    ATCGTCTCAG AGTACATCGA CACATATTTT GAGGATCTGA AGCAGCGTTT GGGTCACAGG
    CTTCAGATCA CTGATCTGCT CATTAAACCT GTACAGAGAA TCATGAAGTA CCAGCTGCTG
    CTGAAGGATT TCCTCAAATT TTCCAAAAAA ATCGGAACAG ATTCGATTGA ATTAGAGAAA
    GCGGTTGAGG TCATGTGCAT TGTACCAAAA AGATGCAACG ACATGATGAA TGTTGGTCGA
    CTCCAGGGGT TTGATGGAAA GATTGTTGCT CAGGGGCGTC TCCTACTCCA GGACACCTTC
    ATGGTGGCCG AACCAGAGGG AGGCCTGCTG AACAGGATGA AGGAGAGGAG AGTCTTCCTC
    TTTGAGCAGA TCGTCATCTT CAGTGAACCT CTAGATAAAA AGAGGGGCTT CTCCATGCCT
    GGCTATTTGT ACAAATACAG CATCAAGGTG AACTGTCTGG GACTTGAGGA CAGTGTGGAC
    GGAGACCCCT GTAAATTTGC ACTGACCTCC AGAACGTCCA ATAGCAGCAA AGATGCCTTC
    ATTCTGCACT CGAGCCATCC TGGAGTACGA CAAGTCTGGA TGCTGCAGAT CAGCCAAATC
    CTGGAGAGCC AACGCAATTT CCTCAATGCA CTTACATCTC CTATAGACTA CCAGAGGAAC
    CACGTGGAGG GTCCTGGTGT GTCAGGCAGC GGTGTACAGG CTGGAGGCAG CGGGGGGCAG
    ATGATGGCCC CTGGAGGAGG AGTAGGGGTC CCGGCTGGGC CGGGTTCCCG TTCTCGTCCG
    TCCCGTATCC CCCAGCCCTC GCGCTTGCCT CAACCGCTCC GTCATCACTC TCCTGCCCTG
    GGGCCTGGAG CCCACGAGCA TGACGGCCCC GACAAACTAT CAGGTATGTC CCCAAGACCT
    CTCTCCAGGG GTCCCTCACC CTCATGCACC ACAGAGCCCG AGCCAAAGGT GAAGCTTCCT
    GCGAGCCCTC ACCCCAAACA GACGGACTCC CAACAGACAG AAAGCCCAGC CAAAGAGATC
    CCACGAGCCA CTGTGGCCCC GCTGGCTCTT GTCAAACCCA GACCTGGAAC GGTTTCACCA
    ATGGCCTCTC CGTTAGCCAC ACCGGCTTTC AAAGACTCCA TTCCACCCTG TAGTCCAGGC
    CCGAAGACTG GCAGCAGCTC GTTTTGGAGC TCAGTGCCCG CGTCCCCCGC CAGCAGGCCT
    GCTTCCTTCA CCTTCCCCGG GGACGCCTGC GACACGCTGA GCCGACCAAA TCACAACCAG
    AGTCAGCGGC ACTCCACCCA CAGTAAAGAC GCAGACCGTA TGAGCACCTG CTCCTCTACC
    AGTGAGCAGT CTATCCAGTC CACTCAGAGC AATGGGAGTG AAAGCAGCAG CAGCAGTAAT
    ATCTCCACCA TGTTGGTGAC TCAGGACTAT GTGGCTCTGA AAGAGGATGA GATTAATGTG
    GGTCAGGGGG AGGTGGTGCA GATCCTGGCC TCCAATCAGC AGAATATGTT CTTGGTGTTT
    CGTGCCGCTA CTGAGCAGTG TCCTGCCGCC GAGGGCTGGA TCCCCGGATA CGTGCTGGGA
    CACACATCCG CCATCATCCC TGACGCTCCA GACGGAACCA TAAAGAAATC GTCATCATCA
    TGGCACACAT CCCTCCGTAT TCGAAAGAAG TCAGAGAAAA GAGAAAAGGA CAATAAAAAA
    GAGGCCAAGC TGGAAAACGG CTACCGGAAA TCCAGAGAGG GACTGACCAA CAAGGTCTCT
    GTGAAGCTTC TAAATCCCAA TTATATATAT GATGTTCTCC CGGAGTTCCT CGTCCCGCTG
    AGTGACGTGA CCTGTGATAA AGGCGAGTGT GTGACTCTGA GGTGTAAAGT GTGTGGTCGG
    CCCAAAGCCA CGGTCACGTG GAAGGGACCT GAGCAGAAAA CACTCACCAA CAACGGCCAC
    TTCAGCATCG CCTACAGTGA GACTGGTGAG GCCACGCTGC GGATCGTAGG TGTGACCTCA
    GAGGATGATG GCAGTTACAC CTGCATTGCC ACGAATGACA TTGGCAGTGT GGCATCATCA
    GCCAGTCTCA GAGTGCTGGG TAAGAACCAC GATGGGATCC GGGTCATGTG GAAAGACAAC
    TTTGAGTCCT TCTACACTGA GGTAGCAGAG CTTGGCAGGG GACGGTTCTC TGTAGTGAAA
    CGCTGTGACC AGAGGGGATC AAAGCGGACC GTGGCGGTCA AGTTTGTCAA TAAGAAACTG
    ATGAAGCGAG ACCAGGTGAC CCATGAGTTC AGTGTCCTAC AGAGACTCCA GCACCCTCAT
    CTGGTCAGAC TGCTGGACAC CTTCGAGACC TCCAGCAGCT ACGCACTGGT CCTGGAGATG
    TCTGATCAGG GCCGTCTGCT GGACTACATC GTGAGCTGGG GGAATCTCAC TGAGGAGAAA
    GTGGCCTTTT ACCTCCGGGA CATTTTAGAA GCTTTGCAGT ACCTGCACAA CTGCAGAATA
    GTCCATCTGG ATCTAAAGCC TGAGAATCTG GTGGTGGAGC AGAGCCCGTC TCAGCCGCTG
    GTCAAGCTGA CGGACTTCGG TGATGCTGTG CAGCTCAACT CCACGCCGTA CGTTCACCCT
    CTGCTGGGGA GCCCGGAGTT CGCAGCCCCT GAGCTGGTCC TCGGGGACCC TGTCTCTCTG
    TCCTCCGACC TGTGGAGTCT GGGGGTCCTG ACGTACGTCA TGCTGAGCGG AGCATCTCCG
    TTCCTTGATG AGAGCGTGGA GGAGACCTGC CTCAACATCT GCCGCTTGGA CTTCAGCTTT
    CCTGATGACT ACTTCCAGGG AGTGAGTCAG GCGGCGCGTG ATTTTATGTG CCTGCTGCTC
    CGGATGGAGC CATCCAAACG TCCACCAGCC ACCTCATGTC TACAGGAGCC CTGGTTGAGA
    GCCGGCGGGG GCCGACGCTC AGCCGAATGC ATCGATACCT CCAGACTGAT CTCCTTCATC
    GACCGACGCA AACACCAGAA CGACCTGCGG CCTCTCACTG GCATCAGAGC CTTCCTGCAG
    ACTCGATTAC AGCCCAGGAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150087
    Clone ID Related Accession (Same CDS sequence) XM_021468075.1
    Accession Version XM_021468075.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGGTATGT CCCCAAGACC TCTCTCCAGG GGTCCCTCAC CCTCATGCAC CACAGAGCCC
    GAGCCAAAGG TGAAGCTTCC TGCGAGCCCT CACCCCAAAC AGACGGACTC CCAACAGACA
    GAAAGCCCAG CCAAAGAGAT CCCACGAGCC ACTGTGGCCC CGCTGGCTCT TGTCAAACCC
    AGACCTGGAA CGGTTTCACC AATGGCCTCT CCGTTAGCCA CACCGGCTTT CAAAGACTCC
    ATTCCACCCT GTAGTCCAGG CCCGAAGACT GGCAGCAGCT CGTTTTGGAG CTCAGTGCCC
    GCGTCCCCCG CCAGCAGGCC TGCTTCCTTC ACCTTCCCCG GGGACGCCTG CGACACGCTG
    AGCCGACCAA ATCACAACCA GAGTCAGCGG CACTCCACCC ACAGTAAAGA CGCAGACCGT
    ATGAGCACCT GCTCCTCTAC CAGTGAGCAG TCTATCCAGT CCACTCAGAG CAATGGGAGT
    GAAAGCAGCA GCAGCAGTAA TATCTCCACC ATGTTGGTGA CTCAGGACTA TGTGGCTCTG
    AAAGAGGATG AGATTAATGT GGGTCAGGGG GAGGTGGTGC AGATCCTGGC CTCCAATCAG
    CAGAATATGT TCTTGGTGTT TCGTGCCGCT ACTGAGCAGT GTCCTGCCGC CGAGGGCTGG
    ATCCCCGGAT ACGTGCTGGG ACACACATCC GCCATCATCC CTGACGCTCC AGACGGAACC
    ATAAACAGGA AATCGTCATC ATCATGGCAC ACATCCCTCC GTATTCGAAA GAAGTCAGAG
    AAAAGAGAAA AGGACAATAA AAAAGAGGCC AAGCTGGAAA ACGGCTACCG GAAATCCAGA
    GAGGGACTGA CCAACAAGGT CTCTGTGAAG CTTCTAAATC CCAATTATAT ATATGATGTT
    CTCCCGGAGT TCCTCGTCCC GCTGAGTGAC GTGACCTGTG ATAAAGGCGA GTGTGTGACT
    CTGAGGTGTA AAGTGTGTGG TCGGCCCAAA GCCACGGTCA CGTGGAAGGG ACCTGAGCAG
    AAAACACTCA CCAACAACGG CCACTTCAGC ATCGCCTACA GTGAGACTGG TGAGGCCACG
    CTGCGGATCG TAGGTGTGAC CTCAGAGGAT GATGGCAGTT ACACCTGCAT TGCCACGAAT
    GACATTGGCA GTGTGGCATC ATCAGCCAGT CTCAGAGTGC TGGGCACGAC CAGCGATGGG
    ATCCGGGTCA TGTGGAAAGA CAACTTTGAG TCCTTCTACA CTGAGGTAGC AGAGCTTGGC
    AGGGGACGGT TCTCTGTAGT GAAACGCTGT GACCAGAGGG GATCAAAGCG GACCGTGGCG
    GTCAAGTTTG TCAATAAGAA ACTGATGAAG CGAGACCAGG TGACCCATGA GTTCAGTGTC
    CTACAGAGAC TCCAGCACCC TCATCTGGTC AGACTGCTGG ACACCTTCGA GACCTCCAGC
    AGCTACGCAC TGGTCCTGGA GATGTCTGAT CAGGGCCGTC TGCTGGACTA CATCGTGAGC
    TGGGGGAATC TCACTGAGGA GAAAGTGGCC TTTTACCTCC GGGACATTTT AGAAGCTTTG
    CAGTACCTGC ACAACTGCAG AATAGTCCAT CTGGATCTAA AGCCTGAGAA TCTGGTGGTG
    GAGCAGAGCC CGTCTCAGCC GCTGGTCAAG CTGACGGACT TCGGTGATGC TGTGCAGCTC
    AACTCCACGC CGTACGTTCA CCCTCTGCTG GGGAGCCCGG AGTTCGCAGC CCCTGAGCTG
    GTCCTCGGGG ACCCTGTCTC TCTGTCCTCC GACCTGTGGA GTCTGGGGGT CCTGACGTAC
    GTCATGCTGA GCGGAGCATC TCCGTTCCTT GATGAGAGCG TGGAGGAGAC CTGCCTCAAC
    ATCTGCCGCT TGGACTTCAG CTTTCCTGAT GACTACTTCC AGGGAGTGAG TCAGGCGGCG
    CGTGATTTTA TGTGCCTGCT GCTCCGGATG GAGCCATCCA AACGTCCACC AGCCACCTCA
    TGTCTACAGG AGCCCTGGTT GAGAGCCGGC GGGGGCCGAC GCTCAGCCGA ATGCATCGAT
    ACCTCCAGAC TGATCTCCTT CATCGACCGA CGCAAACACC AGAACGACCT GCGGCCTCTC
    ACTGGCATCA GAGCCTTCCT GCAGACTCGA TTACAGCCCA GGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323750.1
    CDS192..9539
    Translation

    Target ORF information:

    RefSeq Version XM_021468075.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468075.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGGTATGT CCCCAAGACC TCTCTCCAGG GGTCCCTCAC CCTCATGCAC CACAGAGCCC
    GAGCCAAAGG TGAAGCTTCC TGCGAGCCCT CACCCCAAAC AGACGGACTC CCAACAGACA
    GAAAGCCCAG CCAAAGAGAT CCCACGAGCC ACTGTGGCCC CGCTGGCTCT TGTCAAACCC
    AGACCTGGAA CGGTTTCACC AATGGCCTCT CCGTTAGCCA CACCGGCTTT CAAAGACTCC
    ATTCCACCCT GTAGTCCAGG CCCGAAGACT GGCAGCAGCT CGTTTTGGAG CTCAGTGCCC
    GCGTCCCCCG CCAGCAGGCC TGCTTCCTTC ACCTTCCCCG GGGACGCCTG CGACACGCTG
    AGCCGACCAA ATCACAACCA GAGTCAGCGG CACTCCACCC ACAGTAAAGA CGCAGACCGT
    ATGAGCACCT GCTCCTCTAC CAGTGAGCAG TCTATCCAGT CCACTCAGAG CAATGGGAGT
    GAAAGCAGCA GCAGCAGTAA TATCTCCACC ATGTTGGTGA CTCAGGACTA TGTGGCTCTG
    AAAGAGGATG AGATTAATGT GGGTCAGGGG GAGGTGGTGC AGATCCTGGC CTCCAATCAG
    CAGAATATGT TCTTGGTGTT TCGTGCCGCT ACTGAGCAGT GTCCTGCCGC CGAGGGCTGG
    ATCCCCGGAT ACGTGCTGGG ACACACATCC GCCATCATCC CTGACGCTCC AGACGGAACC
    ATAAACAGGA AATCGTCATC ATCATGGCAC ACATCCCTCC GTATTCGAAA GAAGTCAGAG
    AAAAGAGAAA AGGACAATAA AAAAGAGGCC AAGCTGGAAA ACGGCTACCG GAAATCCAGA
    GAGGGACTGA CCAACAAGGT CTCTGTGAAG CTTCTAAATC CCAATTATAT ATATGATGTT
    CTCCCGGAGT TCCTCGTCCC GCTGAGTGAC GTGACCTGTG ATAAAGGCGA GTGTGTGACT
    CTGAGGTGTA AAGTGTGTGG TCGGCCCAAA GCCACGGTCA CGTGGAAGGG ACCTGAGCAG
    AAAACACTCA CCAACAACGG CCACTTCAGC ATCGCCTACA GTGAGACTGG TGAGGCCACG
    CTGCGGATCG TAGGTGTGAC CTCAGAGGAT GATGGCAGTT ACACCTGCAT TGCCACGAAT
    GACATTGGCA GTGTGGCATC ATCAGCCAGT CTCAGAGTGC TGGGCACGAC CAGCGATGGG
    ATCCGGGTCA TGTGGAAAGA CAACTTTGAG TCCTTCTACA CTGAGGTAGC AGAGCTTGGC
    AGGGGACGGT TCTCTGTAGT GAAACGCTGT GACCAGAGGG GATCAAAGCG GACCGTGGCG
    GTCAAGTTTG TCAATAAGAA ACTGATGAAG CGAGACCAGG TGACCCATGA GTTCAGTGTC
    CTACAGAGAC TCCAGCACCC TCATCTGGTC AGACTGCTGG ACACCTTCGA GACCTCCAGC
    AGCTACGCAC TGGTCCTGGA GATGTCTGAT CAGGGCCGTC TGCTGGACTA CATCGTGAGC
    TGGGGGAATC TCACTGAGGA GAAAGTGGCC TTTTACCTCC GGGACATTTT AGAAGCTTTG
    CAGTACCTGC ACAACTGCAG AATAGTCCAT CTGGATCTAA AGCCTGAGAA TCTGGTGGTG
    GAGCAGAGCC CGTCTCAGCC GCTGGTCAAG CTGACGGACT TCGGTGATGC TGTGCAGCTC
    AACTCCACGC CGTACGTTCA CCCTCTGCTG GGGAGCCCGG AGTTCGCAGC CCCTGAGCTG
    GTCCTCGGGG ACCCTGTCTC TCTGTCCTCC GACCTGTGGA GTCTGGGGGT CCTGACGTAC
    GTCATGCTGA GCGGAGCATC TCCGTTCCTT GATGAGAGCG TGGAGGAGAC CTGCCTCAAC
    ATCTGCCGCT TGGACTTCAG CTTTCCTGAT GACTACTTCC AGGGAGTGAG TCAGGCGGCG
    CGTGATTTTA TGTGCCTGCT GCTCCGGATG GAGCCATCCA AACGTCCACC AGCCACCTCA
    TGTCTACAGG AGCCCTGGTT GAGAGCCGGC GGGGGCCGAC GCTCAGCCGA ATGCATCGAT
    ACCTCCAGAC TGATCTCCTT CATCGACCGA CGCAAACACC AGAACGACCT GCGGCCTCTC
    ACTGGCATCA GAGCCTTCCT GCAGACTCGA TTACAGCCCA GGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150088
    Clone ID Related Accession (Same CDS sequence) XM_021468076.1
    Accession Version XM_021468076.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
    1381
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    1501
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    1981
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    9001
    9061
    9121
    9181
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    9301
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGGTATGT CCCCAAGACC TCTCTCCAGG GGTCCCTCAC CCTCATGCAC CACAGAGCCC
    GAGCCAAAGG TGAAGCTTCC TGCGAGCCCT CACCCCAAAC AGACGGACTC CCAACAGACA
    GAAAGCCCAG CCAAAGAGAT CCCACGAGCC ACTGTGGCCC CGCTGGCTCT TGTCAAACCC
    AGACCTGGAA CGGTTTCACC AATGGCCTCT CCGTTAGCCA CACCGGCTTT CAAAGACTCC
    ATTCCACCCT GTAGTCCAGG CCCGAAGACT GGCAGCAGCT CGTTTTGGAG CTCAGTGCCC
    GCGTCCCCCG CCAGCAGGCC TGCTTCCTTC ACCTTCCCCG GGGACGCCTG CGACACGCTG
    AGCCGACCAA ATCACAACCA GAGTCAGCGG CACTCCACCC ACAGTAAAGA CGCAGACCGT
    ATGAGCACCT GCTCCTCTAC CAGTGAGCAG TCTATCCAGT CCACTCAGAG CAATGGGAGT
    GAAAGCAGCA GCAGCAGTAA TATCTCCACC ATGTTGGTGA CTCAGGACTA TGTGGCTCTG
    AAAGAGGATG AGATTAATGT GGGTCAGGGG GAGGTGGTGC AGATCCTGGC CTCCAATCAG
    CAGAATATGT TCTTGGTGTT TCGTGCCGCT ACTGAGCAGT GTCCTGCCGC CGAGGGCTGG
    ATCCCCGGAT ACGTGCTGGG ACACACATCC GCCATCATCC CTGACGCTCC AGACGGAACC
    ATAAAGAAAT CGTCATCATC ATGGCACACA TCCCTCCGTA TTCGAAAGAA GTCAGAGAAA
    AGAGAAAAGG ACAATAAAAA AGAGGCCAAG CTGGAAAACG GCTACCGGAA ATCCAGAGAG
    GGACTGACCA ACAAGGTCTC TGTGAAGCTT CTAAATCCCA ATTATATATA TGATGTTCTC
    CCGGAGTTCC TCGTCCCGCT GAGTGACGTG ACCTGTGATA AAGGCGAGTG TGTGACTCTG
    AGGTGTAAAG TGTGTGGTCG GCCCAAAGCC ACGGTCACGT GGAAGGGACC TGAGCAGAAA
    ACACTCACCA ACAACGGCCA CTTCAGCATC GCCTACAGTG AGACTGGTGA GGCCACGCTG
    CGGATCGTAG GTGTGACCTC AGAGGATGAT GGCAGTTACA CCTGCATTGC CACGAATGAC
    ATTGGCAGTG TGGCATCATC AGCCAGTCTC AGAGTGCTGG GCACGACCAG CGATGGGATC
    CGGGTCATGT GGAAAGACAA CTTTGAGTCC TTCTACACTG AGGTAGCAGA GCTTGGCAGG
    GGACGGTTCT CTGTAGTGAA ACGCTGTGAC CAGAGGGGAT CAAAGCGGAC CGTGGCGGTC
    AAGTTTGTCA ATAAGAAACT GATGAAGCGA GACCAGGTGA CCCATGAGTT CAGTGTCCTA
    CAGAGACTCC AGCACCCTCA TCTGGTCAGA CTGCTGGACA CCTTCGAGAC CTCCAGCAGC
    TACGCACTGG TCCTGGAGAT GTCTGATCAG GGCCGTCTGC TGGACTACAT CGTGAGCTGG
    GGGAATCTCA CTGAGGAGAA AGTGGCCTTT TACCTCCGGG ACATTTTAGA AGCTTTGCAG
    TACCTGCACA ACTGCAGAAT AGTCCATCTG GATCTAAAGC CTGAGAATCT GGTGGTGGAG
    CAGAGCCCGT CTCAGCCGCT GGTCAAGCTG ACGGACTTCG GTGATGCTGT GCAGCTCAAC
    TCCACGCCGT ACGTTCACCC TCTGCTGGGG AGCCCGGAGT TCGCAGCCCC TGAGCTGGTC
    CTCGGGGACC CTGTCTCTCT GTCCTCCGAC CTGTGGAGTC TGGGGGTCCT GACGTACGTC
    ATGCTGAGCG GAGCATCTCC GTTCCTTGAT GAGAGCGTGG AGGAGACCTG CCTCAACATC
    TGCCGCTTGG ACTTCAGCTT TCCTGATGAC TACTTCCAGG GAGTGAGTCA GGCGGCGCGT
    GATTTTATGT GCCTGCTGCT CCGGATGGAG CCATCCAAAC GTCCACCAGC CACCTCATGT
    CTACAGGAGC CCTGGTTGAG AGCCGGCGGG GGCCGACGCT CAGCCGAATG CATCGATACC
    TCCAGACTGA TCTCCTTCAT CGACCGACGC AAACACCAGA ACGACCTGCG GCCTCTCACT
    GGCATCAGAG CCTTCCTGCA GACTCGATTA CAGCCCAGGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323751.1
    CDS193..9537
    Translation

    Target ORF information:

    RefSeq Version XM_021468076.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468076.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGGTATGT CCCCAAGACC TCTCTCCAGG GGTCCCTCAC CCTCATGCAC CACAGAGCCC
    GAGCCAAAGG TGAAGCTTCC TGCGAGCCCT CACCCCAAAC AGACGGACTC CCAACAGACA
    GAAAGCCCAG CCAAAGAGAT CCCACGAGCC ACTGTGGCCC CGCTGGCTCT TGTCAAACCC
    AGACCTGGAA CGGTTTCACC AATGGCCTCT CCGTTAGCCA CACCGGCTTT CAAAGACTCC
    ATTCCACCCT GTAGTCCAGG CCCGAAGACT GGCAGCAGCT CGTTTTGGAG CTCAGTGCCC
    GCGTCCCCCG CCAGCAGGCC TGCTTCCTTC ACCTTCCCCG GGGACGCCTG CGACACGCTG
    AGCCGACCAA ATCACAACCA GAGTCAGCGG CACTCCACCC ACAGTAAAGA CGCAGACCGT
    ATGAGCACCT GCTCCTCTAC CAGTGAGCAG TCTATCCAGT CCACTCAGAG CAATGGGAGT
    GAAAGCAGCA GCAGCAGTAA TATCTCCACC ATGTTGGTGA CTCAGGACTA TGTGGCTCTG
    AAAGAGGATG AGATTAATGT GGGTCAGGGG GAGGTGGTGC AGATCCTGGC CTCCAATCAG
    CAGAATATGT TCTTGGTGTT TCGTGCCGCT ACTGAGCAGT GTCCTGCCGC CGAGGGCTGG
    ATCCCCGGAT ACGTGCTGGG ACACACATCC GCCATCATCC CTGACGCTCC AGACGGAACC
    ATAAAGAAAT CGTCATCATC ATGGCACACA TCCCTCCGTA TTCGAAAGAA GTCAGAGAAA
    AGAGAAAAGG ACAATAAAAA AGAGGCCAAG CTGGAAAACG GCTACCGGAA ATCCAGAGAG
    GGACTGACCA ACAAGGTCTC TGTGAAGCTT CTAAATCCCA ATTATATATA TGATGTTCTC
    CCGGAGTTCC TCGTCCCGCT GAGTGACGTG ACCTGTGATA AAGGCGAGTG TGTGACTCTG
    AGGTGTAAAG TGTGTGGTCG GCCCAAAGCC ACGGTCACGT GGAAGGGACC TGAGCAGAAA
    ACACTCACCA ACAACGGCCA CTTCAGCATC GCCTACAGTG AGACTGGTGA GGCCACGCTG
    CGGATCGTAG GTGTGACCTC AGAGGATGAT GGCAGTTACA CCTGCATTGC CACGAATGAC
    ATTGGCAGTG TGGCATCATC AGCCAGTCTC AGAGTGCTGG GCACGACCAG CGATGGGATC
    CGGGTCATGT GGAAAGACAA CTTTGAGTCC TTCTACACTG AGGTAGCAGA GCTTGGCAGG
    GGACGGTTCT CTGTAGTGAA ACGCTGTGAC CAGAGGGGAT CAAAGCGGAC CGTGGCGGTC
    AAGTTTGTCA ATAAGAAACT GATGAAGCGA GACCAGGTGA CCCATGAGTT CAGTGTCCTA
    CAGAGACTCC AGCACCCTCA TCTGGTCAGA CTGCTGGACA CCTTCGAGAC CTCCAGCAGC
    TACGCACTGG TCCTGGAGAT GTCTGATCAG GGCCGTCTGC TGGACTACAT CGTGAGCTGG
    GGGAATCTCA CTGAGGAGAA AGTGGCCTTT TACCTCCGGG ACATTTTAGA AGCTTTGCAG
    TACCTGCACA ACTGCAGAAT AGTCCATCTG GATCTAAAGC CTGAGAATCT GGTGGTGGAG
    CAGAGCCCGT CTCAGCCGCT GGTCAAGCTG ACGGACTTCG GTGATGCTGT GCAGCTCAAC
    TCCACGCCGT ACGTTCACCC TCTGCTGGGG AGCCCGGAGT TCGCAGCCCC TGAGCTGGTC
    CTCGGGGACC CTGTCTCTCT GTCCTCCGAC CTGTGGAGTC TGGGGGTCCT GACGTACGTC
    ATGCTGAGCG GAGCATCTCC GTTCCTTGAT GAGAGCGTGG AGGAGACCTG CCTCAACATC
    TGCCGCTTGG ACTTCAGCTT TCCTGATGAC TACTTCCAGG GAGTGAGTCA GGCGGCGCGT
    GATTTTATGT GCCTGCTGCT CCGGATGGAG CCATCCAAAC GTCCACCAGC CACCTCATGT
    CTACAGGAGC CCTGGTTGAG AGCCGGCGGG GGCCGACGCT CAGCCGAATG CATCGATACC
    TCCAGACTGA TCTCCTTCAT CGACCGACGC AAACACCAGA ACGACCTGCG GCCTCTCACT
    GGCATCAGAG CCTTCCTGCA GACTCGATTA CAGCCCAGGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150089
    Clone ID Related Accession (Same CDS sequence) XM_021468077.1
    Accession Version XM_021468077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGGTATGT CCCCAAGACC TCTCTCCAGG GGTCCCTCAC CCTCATGCAC CACAGAGCCC
    GAGCCAAAGG TGAAGCTTCC TGCGAGCCCT CACCCCAAAC AGACGGACTC CCAACAGACA
    GAAAGCCCAG CCAAAGAGAT CCCACGAGCC ACTGTGGCCC CGCTGGCTCT TGTCAAACCC
    AGACCTGGAA CGGTTTCACC AATGGCCTCT CCGTTAGCCA CACCGGCTTT CAAAGACTCC
    ATTCCACCCT GTAGTCCAGG CCCGAAGACT GGCAGCAGCT CGTTTTGGAG CTCAGTGCCC
    GCGTCCCCCG CCAGCAGGCC TGCTTCCTTC ACCTTCCCCG GGGACGCCTG CGACACGCTG
    AGCCGACCAA ATCACAACCA GAGTCAGCGG CACTCCACCC ACAGTAAAGA CGCAGACCGT
    ATGAGCACCT GCTCCTCTAC CAGTGAGCAG TCTATCCAGT CCACTCAGAG CAATGGGAGT
    GAAAGCAGCA GCAGCAGTAA TATCTCCACC ATGTTGGTGA CTCAGGACTA TGTGGCTCTG
    AAAGAGGATG AGATTAATGT GGGTCAGGGG GAGGTGGTGC AGATCCTGGC CTCCAATCAG
    CAGAATATGT TCTTGGTGTT TCGTGCCGCT ACTGAGCAGT GTCCTGCCGC CGAGGGCTGG
    ATCCCCGGAT ACGTGCTGGG ACACACATCC GCCATCATCC CTGACGCTCC AGACGGAACC
    ATAAACAGGA AATCGTCATC ATCATGGCAC ACATCCCTCC GTATTCGAAA GAAGTCAGAG
    AAAAGAGAAA AGGACAATAA AAAAGAGGCC AAGCTGGAAA ACGGCTACCG GAAATCCAGA
    GAGGGACTGA CCAACAAGCT TCTAAATCCC AATTATATAT ATGATGTTCT CCCGGAGTTC
    CTCGTCCCGC TGAGTGACGT GACCTGTGAT AAAGGCGAGT GTGTGACTCT GAGGTGTAAA
    GTGTGTGGTC GGCCCAAAGC CACGGTCACG TGGAAGGGAC CTGAGCAGAA AACACTCACC
    AACAACGGCC ACTTCAGCAT CGCCTACAGT GAGACTGGTG AGGCCACGCT GCGGATCGTA
    GGTGTGACCT CAGAGGATGA TGGCAGTTAC ACCTGCATTG CCACGAATGA CATTGGCAGT
    GTGGCATCAT CAGCCAGTCT CAGAGTGCTG GGCACGACCA GCGATGGGAT CCGGGTCATG
    TGGAAAGACA ACTTTGAGTC CTTCTACACT GAGGTAGCAG AGCTTGGCAG GGGACGGTTC
    TCTGTAGTGA AACGCTGTGA CCAGAGGGGA TCAAAGCGGA CCGTGGCGGT CAAGTTTGTC
    AATAAGAAAC TGATGAAGCG AGACCAGGTG ACCCATGAGT TCAGTGTCCT ACAGAGACTC
    CAGCACCCTC ATCTGGTCAG ACTGCTGGAC ACCTTCGAGA CCTCCAGCAG CTACGCACTG
    GTCCTGGAGA TGTCTGATCA GGGCCGTCTG CTGGACTACA TCGTGAGCTG GGGGAATCTC
    ACTGAGGAGA AAGTGGCCTT TTACCTCCGG GACATTTTAG AAGCTTTGCA GTACCTGCAC
    AACTGCAGAA TAGTCCATCT GGATCTAAAG CCTGAGAATC TGGTGGTGGA GCAGAGCCCG
    TCTCAGCCGC TGGTCAAGCT GACGGACTTC GGTGATGCTG TGCAGCTCAA CTCCACGCCG
    TACGTTCACC CTCTGCTGGG GAGCCCGGAG TTCGCAGCCC CTGAGCTGGT CCTCGGGGAC
    CCTGTCTCTC TGTCCTCCGA CCTGTGGAGT CTGGGGGTCC TGACGTACGT CATGCTGAGC
    GGAGCATCTC CGTTCCTTGA TGAGAGCGTG GAGGAGACCT GCCTCAACAT CTGCCGCTTG
    GACTTCAGCT TTCCTGATGA CTACTTCCAG GGAGTGAGTC AGGCGGCGCG TGATTTTATG
    TGCCTGCTGC TCCGGATGGA GCCATCCAAA CGTCCACCAG CCACCTCATG TCTACAGGAG
    CCCTGGTTGA GAGCCGGCGG GGGCCGACGC TCAGCCGAAT GCATCGATAC CTCCAGACTG
    ATCTCCTTCA TCGACCGACG CAAACACCAG AACGACCTGC GGCCTCTCAC TGGCATCAGA
    GCCTTCCTGC AGACTCGATT ACAGCCCAGG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323752.1
    CDS193..9528
    Translation

    Target ORF information:

    RefSeq Version XM_021468077.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTAC AGAGCCGCGC TGCTCCAAAT CACTACTCTG AAAGAGCCTT TAAGAGAACA
    CATCTTGAAG TGAGCCAAGA CGGAAAAGCC TTATTGGACA AACTTCAGCG CCCCTTGACG
    CCCGGCAGTG CTGATTCGCT GACAGCTTCC GCCAATTACA CCAAGGCGGT GAACCATGTT
    TTGGACATCA TCCATGAGGT TCTGCATCAC CAAAGGCAGC TGGAGAACAT CTGGCAACAT
    CGCAAGGTCC GGCTGCACCA GCGTCTGCAG CTGTGTGTTT TCCAGCAGGA CGTTCAGCAG
    GTTCTGGACT GGATTGAGAA CCATGGCGAG GCATTTCTCA GCAAGCACAC AGGGGTGGGA
    AAATCTCTGC ATCGCGCTCG AGCTCTGCAG AAACGCCATG AAGACTTTGA GGAAGTGGCT
    CAGAACACGT ACACAAATGC GGATAAGCTT CTGGAAGCGG CAGAGCAGCT GGCCCAGACA
    GGCGAGTGTG ACCCAGAGGA GATCTATCAG GCCGCTCATC AGCTGGAGGA CCGAATCCAG
    GACTTTGTTC GGCGCGTGGA GCAGCGCAAG GTCCTGCTGG ACATGTCAGT GGCTTTCCAC
    ACTCACGTCA AAGAGTTGTG GACGTGGCTG GAGGAGCTGC AGAAGGAGTT GCTGGACGAT
    GTGTACGCCG AGTCTGTGGA AGCCGTGCAG GATCTGATCA AGCGTTTCGG GCAGCAGCAA
    CAAACCACAC TGCAGTTCAC TGTAAACGTC ATCAAAGAAG GAGAAGATCT CATCCAGCAG
    CTCAGAGACC TGGCCATCTC CAGCAACAAG ACGCCTCACA ACAGCTCCAT CAACCACATC
    GAGAGCGTCC TGCAGCAGCT GGATGAGGCT CAGGCTCAGA TGGAGGAGCT TTTCCAGGAG
    AGGAAGATCA AGCTGGAGCT TTTCCTGCAG CTCAGGATCT TTGAGCGGGA CGCCATAGAT
    ATTATCTCAG ATCTGGACTC GTGGAATGAG GAGCTGTCGC AGCAGATGAA TGAGTTTGAT
    ACAGAAGACC TGACGCTGGC TGAACAGAGA CTGCAGCACC ATGCTGATAA AGCCCTGACC
    ATGAACAACC TGGCCTTCCA CGTCATCCAC CAGGGCCAGG AACTGCTGCA GTACGTCAAC
    GAGGTGCAGG CGTCTGGTGT GGAGTTGCTG TGTGACCGTG ATGTGGACAT GGCCACCCGT
    GTTCAGGACC TGCTAGAGTT TCTACATGAG AAACAGCAAG AGCTGGACGT GGCTGCAGAA
    CAGCATCGCA GACACCTTGA GCAGTGTGTG CAACTGCGCC ACCTACAAGC CGAAGTCAAA
    CAGGTTCTGG GTTGGATCCG TAATGGCGAG TCGATGCTGA ATGCAGGTCT GATTACGGCC
    AGCTCTCTTC AAGAAGCAGA GCAGTTGCAG AGAGAGCATG AGCAATTCCA GCATGCTATC
    GAGAAAACCC ATCAGAGCGC CCTGCAGGTC CAGCAGAAAG CCGAAGCCCT GCTGCAGGCC
    AATCACTACG ACATGGACAT GATTCGAGAC TGCGCGGAGA ACGTGGCTTC CCACTGGCAG
    CAGCTCATGC TGAAGATGGA GGACCGGCTC AAGCTGGTCA ACGCCTCCGT GGCCTTCTAT
    AAGACCTCTG AACAGGTTTG CAGTGTTCTG GAGAGTCTGG AGCAGGAGTA CAAGCGTGAG
    GAGGACTGGT GCGGAGGAGC TGACAAACTG GGACCCAACT GTGAATCGGA CCACGTCACA
    CCCATGATCA GCAAACACCT GGAGCAGAAA GAGGCTTTCC TCAAGGCCTG CACGTTGGCC
    AGGCGTAATG CAGACGTCTT CCTCAAATAC ATGCACAGGA ACAGCGTCAG CATGCCCGGG
    ATGTTGAGTC ACGTCAAAGC TCCAGAGCAG CAGGTCAAAA ATATCCTGAA TGAGCTGCTG
    CAGAGGGAGA ACCGCGTGCT GCACTTCTGG ACTATGAGAA AGAGGAGGCT GGACCAGTGT
    CAGCAATACG TGGTGTTTGA ACGCAGCGCC AAACAGGCTC TGGAGTGGAT CCACGACACT
    GGCGAGTTTT ATCTGTCCAC ACACACGTCC ACCGGCTCCA GCATACACCA CACACAGGAG
    CTGCTGAAGG AGCACGAGGA CTTCCACATC ACAGCCAAGC AAACCAAAGA ACGGGTGAAG
    CTGCTGATCC AGCTGGCAGA TGGTTTCTGT GATAAGGGTC ACTCTCACGC CGCTGAGATT
    AAGAAATGGG TGACAGCGGT GGACAAACGC TATCGAGATT TCTCGCTCCG CATGGACAAG
    TACCGCACAT CTCTGGAGAA AGCACTCGGC ATCTCCTCAG ACTCCAATAA AGCTAGCAAA
    GACTTGCAGC TGGACATCAT CCCTGCCAGT GCTCCGGGGT CAGAGGTCAA ACTCAGAGAC
    GCTGCTCATG AGCTCAATGA AGAGAAGCGC AAGTCTGCCC GCAGGAAGGA CTTCATCATG
    GCTGAGCTGA TCCAGACAGA AAAGGCCTAT GTGCGAGACT TGAGAGAGTG CATGGACACG
    TATCTGTGGG AGATGACCAG CGGCGTGGAG GAGATCCCAC CAGGCATCGT CAACAAAGAG
    CACATCATTT TTGGTAACAT GCAAGATCTG TACGAGTTCC ATCACAATAT ATTCCTCAAA
    GAGCTGGAGA AATACGAGCA GCTTCCTGAA GATGTGGGCC ACTGTTTTGT GACTTGGGCT
    GATAAGTTCC AGATGTATGT GAACTACTGC AAGAACAAAC CAGACTCCAC TCAGCTTATT
    CTTGAACATG CCGGAGGATA CTTCGACGAG ATCCAGCAGA GGCATCGCCT GGCCAACTCC
    ATCTCCTCAT ACCTCATCAA ACCTGTTCAG AGAATAACCA AGTACCAGCT GCTCCTCAAG
    GAGCTCCTGA CGTGCTGCGA GGAAGGCAAA GGTGAAATTA AAGATGGCCT GGAGGTGATG
    CTCAGTGTTC CCAAAAGAGC CAACGACGCC ATGCACTTGA GCATGCTTGA AGGGTTTGAT
    GAGAACATCG AGTCCCAGGG AGAGCTGATC CTGCAGGAGG CGTTTCAGGT GTGGGATCCC
    AAAACTCTGA TCCGCAAGGG AAGAGAACGA CACCTGTTCC TCTTCGAGAT GTCACTGATC
    TTCAGCAAGG AGGTCAAAGA CTCCAATGGC AGGAGCAAGT ACATCTACAA GAGCAAGCTT
    TTCACCTCTG AACTGGGTGT CACTGAGCAT GTGGAGGGAG ATCCATGCAA ATTTGCTCTC
    TGGGTTGGAC GAACCCCAAC TTCTGACAAC AAGATTGTCC TCAAGCTAAG TCAGGGATGC
    AAACATGGAT CATTGGCGTC CGGTATTGAG AACAAACAGG ACTGGATCAA GCACATCCGG
    GAAGTGATTC AGGAGCGCAC CGTTCACCTG AAGGGGGCGC TGAAAGAGCC CATTCACATC
    CCCAAAGCCT CCACAGCCAA ACACAAGGGG CGCAGGTACA CCCCAAGGGA TGGAGAGGAT
    CTGGACAGTC AGGGTGACGG CAGCAGTCAA CCAGACACCA TCTCACTGGC CTCCAGGACC
    TCACAGAACA CACTGGACAG CGACAAGTTG TCTGGTGGCT GTGAGCTGAC GGTGGTCATC
    CATGACTTTA TGGCCGGTAA CAGTAATGAG CTGACCATCC GGAGAGGACA GACGGTGGAG
    GTGCTCGAGA GACTTCATGA TAAGCCGGAC TGGTGTTTGG TTCGCACCAC AGACCGTTCG
    CCGGCGCTGG AGGGCCTGGT GCCCTGTGCC ATGCTCTGTA TCGCCCACTC CCGCAGCAGC
    ATGGAGATGG AGGGCATCTT CAACCACAAA GATGCACTCT CTGTATGCAG TAATGATGCC
    ATCATGCCAG GCTCCTCTGC CACCCTGCAG CCGGGTCATG TGATGGGTTC CCAGAGTTCT
    CCTGGACCTA AAAGGCCTGG CAACACATTA AGGAAATGGC TGACCAGTCC TGTGAGGAGA
    CTGAGCAGCG GTAAAGCTGA CGGACACGTC AAGAAGCTGG CACACAAACA CAAGAAGAGC
    CGTGACGTCA GAAAGAGCGC AGACGCCGGT TCACAGAAAG ACTCGGACGA CAGCGCTGCG
    ACGCCACAAG ATGAGACCCT CGAGGAGAGG GTGCGTAATG AGGGTCTGAG CAGCGGCACG
    CTCTCCAAAT CCTCCTCTTC AGGGATGCAG AGCTGTGGAG AGGAAGAGGG AGAGGAAGGA
    GCTGACGCTG TGCCTCTTCC TCCACCAATG GCCATCCAGC AGCACAGCCT CCTCCAGCCG
    GATGCGCAGG ATGACAAGAC GTCTTCACGT CTTTCCGTCA GACCCTCCAG TTCTGAGACC
    CCCAGCGCTG CAGAGCTCGT CAGTGCCATT GAGGAGCTGG TCAAAAGCAA GATGGCTCTG
    GAAGACAGAC CCAGCACTCT GTCAGTGGAG CAGGGCGACA GCAGCAGCCC TTCATTCAAC
    CCATCTGATA ACTCTCTCCT GTCCTCCTCC TCTCCAATCG ATGAGATAGA GGAACGCAAG
    ACCGGTTTCT TCAAGAAGCG CCATTACGTC CTGCTGGAGC TGATCGAGAC GGAGAGAGAC
    TATGTGCGAG ATCTCAGTTT GGTCGTGGAG GGCTACATGG CCAGGATGAG GGAGGACGGG
    GTTCCAGATG ATATGAAAGG AAAAGACAAG ATCGTTTTTG GAAACATTCA GCAGATATAT
    GACTGGCACA AAGATTTCTT CCTGGGAGAG CTTGAGAAGT GCCTTGAGGA CCCTGACCGG
    CTGGGCCCTC TCTTCCTCAA ACATGAGCGG AGACTGCACA TGTACATTGT TTACTGCCAG
    AACAAACCTA AATCTGAACA CATCGTCTCA GAGTACATCG ACACATATTT TGAGGATCTG
    AAGCAGCGTT TGGGTCACAG GCTTCAGATC ACTGATCTGC TCATTAAACC TGTACAGAGA
    ATCATGAAGT ACCAGCTGCT GCTGAAGGAT TTCCTCAAAT TTTCCAAAAA AATCGGAACA
    GATTCGATTG AATTAGAGAA AGCGGTTGAG GTCATGTGCA TTGTACCAAA AAGATGCAAC
    GACATGATGA ATGTTGGTCG ACTCCAGGGG TTTGATGGAA AGATTGTTGC TCAGGGGCGT
    CTCCTACTCC AGGACACCTT CATGGTGGCC GAACCAGAGG GAGGCCTGCT GAACAGGATG
    AAGGAGAGGA GAGTCTTCCT CTTTGAGCAG ATCGTCATCT TCAGTGAACC TCTAGATAAA
    AAGAGGGGCT TCTCCATGCC TGGCTATTTG TACAAATACA GCATCAAGGT GAACTGTCTG
    GGACTTGAGG ACAGTGTGGA CGGAGACCCC TGTAAATTTG CACTGACCTC CAGAACGTCC
    AATAGCAGCA AAGATGCCTT CATTCTGCAC TCGAGCCATC CTGGAGTACG ACAAGTCTGG
    ATGCTGCAGA TCAGCCAAAT CCTGGAGAGC CAACGCAATT TCCTCAATGC ACTTACATCT
    CCTATAGACT ACCAGAGGAA CCACGTGGAG GGTCCTGGTG TGTCAGGCAG CGGTGTACAG
    GCTGGAGGCA GCGGGGGGCA GATGATGGCC CCTGGAGGAG GAGTAGGGGT CCCGGCTGGG
    CCGGGTTCCC GTTCTCGTCC GTCCCGTATC CCCCAGCCCT CGCGCTTGCC TCAACCGCTC
    CGTCATCACT CTCCTGCCCT GGGGCCTGGA GCCCACGAGC ATGACGGCCC CGACAAACTA
    TCAGGTATGT CCCCAAGACC TCTCTCCAGG GGTCCCTCAC CCTCATGCAC CACAGAGCCC
    GAGCCAAAGG TGAAGCTTCC TGCGAGCCCT CACCCCAAAC AGACGGACTC CCAACAGACA
    GAAAGCCCAG CCAAAGAGAT CCCACGAGCC ACTGTGGCCC CGCTGGCTCT TGTCAAACCC
    AGACCTGGAA CGGTTTCACC AATGGCCTCT CCGTTAGCCA CACCGGCTTT CAAAGACTCC
    ATTCCACCCT GTAGTCCAGG CCCGAAGACT GGCAGCAGCT CGTTTTGGAG CTCAGTGCCC
    GCGTCCCCCG CCAGCAGGCC TGCTTCCTTC ACCTTCCCCG GGGACGCCTG CGACACGCTG
    AGCCGACCAA ATCACAACCA GAGTCAGCGG CACTCCACCC ACAGTAAAGA CGCAGACCGT
    ATGAGCACCT GCTCCTCTAC CAGTGAGCAG TCTATCCAGT CCACTCAGAG CAATGGGAGT
    GAAAGCAGCA GCAGCAGTAA TATCTCCACC ATGTTGGTGA CTCAGGACTA TGTGGCTCTG
    AAAGAGGATG AGATTAATGT GGGTCAGGGG GAGGTGGTGC AGATCCTGGC CTCCAATCAG
    CAGAATATGT TCTTGGTGTT TCGTGCCGCT ACTGAGCAGT GTCCTGCCGC CGAGGGCTGG
    ATCCCCGGAT ACGTGCTGGG ACACACATCC GCCATCATCC CTGACGCTCC AGACGGAACC
    ATAAACAGGA AATCGTCATC ATCATGGCAC ACATCCCTCC GTATTCGAAA GAAGTCAGAG
    AAAAGAGAAA AGGACAATAA AAAAGAGGCC AAGCTGGAAA ACGGCTACCG GAAATCCAGA
    GAGGGACTGA CCAACAAGCT TCTAAATCCC AATTATATAT ATGATGTTCT CCCGGAGTTC
    CTCGTCCCGC TGAGTGACGT GACCTGTGAT AAAGGCGAGT GTGTGACTCT GAGGTGTAAA
    GTGTGTGGTC GGCCCAAAGC CACGGTCACG TGGAAGGGAC CTGAGCAGAA AACACTCACC
    AACAACGGCC ACTTCAGCAT CGCCTACAGT GAGACTGGTG AGGCCACGCT GCGGATCGTA
    GGTGTGACCT CAGAGGATGA TGGCAGTTAC ACCTGCATTG CCACGAATGA CATTGGCAGT
    GTGGCATCAT CAGCCAGTCT CAGAGTGCTG GGCACGACCA GCGATGGGAT CCGGGTCATG
    TGGAAAGACA ACTTTGAGTC CTTCTACACT GAGGTAGCAG AGCTTGGCAG GGGACGGTTC
    TCTGTAGTGA AACGCTGTGA CCAGAGGGGA TCAAAGCGGA CCGTGGCGGT CAAGTTTGTC
    AATAAGAAAC TGATGAAGCG AGACCAGGTG ACCCATGAGT TCAGTGTCCT ACAGAGACTC
    CAGCACCCTC ATCTGGTCAG ACTGCTGGAC ACCTTCGAGA CCTCCAGCAG CTACGCACTG
    GTCCTGGAGA TGTCTGATCA GGGCCGTCTG CTGGACTACA TCGTGAGCTG GGGGAATCTC
    ACTGAGGAGA AAGTGGCCTT TTACCTCCGG GACATTTTAG AAGCTTTGCA GTACCTGCAC
    AACTGCAGAA TAGTCCATCT GGATCTAAAG CCTGAGAATC TGGTGGTGGA GCAGAGCCCG
    TCTCAGCCGC TGGTCAAGCT GACGGACTTC GGTGATGCTG TGCAGCTCAA CTCCACGCCG
    TACGTTCACC CTCTGCTGGG GAGCCCGGAG TTCGCAGCCC CTGAGCTGGT CCTCGGGGAC
    CCTGTCTCTC TGTCCTCCGA CCTGTGGAGT CTGGGGGTCC TGACGTACGT CATGCTGAGC
    GGAGCATCTC CGTTCCTTGA TGAGAGCGTG GAGGAGACCT GCCTCAACAT CTGCCGCTTG
    GACTTCAGCT TTCCTGATGA CTACTTCCAG GGAGTGAGTC AGGCGGCGCG TGATTTTATG
    TGCCTGCTGC TCCGGATGGA GCCATCCAAA CGTCCACCAG CCACCTCATG TCTACAGGAG
    CCCTGGTTGA GAGCCGGCGG GGGCCGACGC TCAGCCGAAT GCATCGATAC CTCCAGACTG
    ATCTCCTTCA TCGACCGACG CAAACACCAG AACGACCTGC GGCCTCTCAC TGGCATCAGA
    GCCTTCCTGC AGACTCGATT ACAGCCCAGG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42636
    Clone ID Related Accession (Same CDS sequence) XM_009294415.2
    Accession Version XM_009294415.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294415.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    301
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    541
    601
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    721
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2641
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    3181
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    4141
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    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGACGTC TTCACGTCTT TCCGTCAGAC CCTCCAGTTC TGAGACCCCC
    AGCGCTGCAG AGCTCGTCAG TGCCATTGAG GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA
    GACAGACCCA GCACTCTGTC AGTGGAGCAG GGCGACAGCA GCAGCCCTTC ATTCAACCCA
    TCTGATAACT CTCTCCTGTC CTCCTCCTCT CCAATCGATG AGATAGAGGA ACGCAAGACC
    GGTTTCTTCA AGAAGCGCCA TTACGTCCTG CTGGAGCTGA TCGAGACGGA GAGAGACTAT
    GTGCGAGATC TCAGTTTGGT CGTGGAGGGC TACATGGCCA GGATGAGGGA GGACGGGGTT
    CCAGATGATA TGAAAGGAAA AGACAAGATC GTTTTTGGAA ACATTCAGCA GATATATGAC
    TGGCACAAAG ATTTCTTCCT GGGAGAGCTT GAGAAGTGCC TTGAGGACCC TGACCGGCTG
    GGCCCTCTCT TCCTCAAACA TGAGCGGAGA CTGCACATGT ACATTGTTTA CTGCCAGAAC
    AAACCTAAAT CTGAACACAT CGTCTCAGAG TACATCGACA CATATTTTGA GGATCTGAAG
    CAGCGTTTGG GTCACAGGCT TCAGATCACT GATCTGCTCA TTAAACCTGT ACAGAGAATC
    ATGAAGTACC AGCTGCTGCT GAAGGATTTC CTCAAATTTT CCAAAAAAAT CGGAACAGAT
    TCGATTGAAT TAGAGAAAGC GGTTGAGGTC ATGTGCATTG TACCAAAAAG ATGCAACGAC
    ATGATGAATG TTGGTCGACT CCAGGGGTTT GATGGAAAGA TTGTTGCTCA GGGGCGTCTC
    CTACTCCAGG ACACCTTCAT GGTGGCCGAA CCAGAGGGAG GCCTGCTGAA CAGGATGAAG
    GAGAGGAGAG TCTTCCTCTT TGAGCAGATC GTCATCTTCA GTGAACCTCT AGATAAAAAG
    AGGGGCTTCT CCATGCCTGG CTATTTGTAC AAATACAGCA TCAAGGTGAA CTGTCTGGGA
    CTTGAGGACA GTGTGGACGG AGACCCCTGT AAATTTGCAC TGACCTCCAG AACGTCCAAT
    AGCAGCAAAG ATGCCTTCAT TCTGCACTCG AGCCATCCTG GAGTACGACA AGTCTGGATG
    CTGCAGATCA GCCAAATCCT GGAGAGCCAA CGCAATTTCC TCAATGCACT TACATCTCCT
    ATAGACTACC AGAGGAACCA CGTGGAGGGT CCTGGTGTGT CAGGCAGCGG TGTACAGGCT
    GGAGGCAGCG GGGGGCAGAT GATGGCCCCT GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG
    GGTTCCCGTT CTCGTCCGTC CCGTATCCCC CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT
    CATCACTCTC CTGCCCTGGG GCCTGGAGCC CACGAGCATG ACGGCCCCGA CAAACTATCA
    GGTATGTCCC CAAGACCTCT CTCCAGGGGT CCCTCACCCT CATGCACCAC AGAGCCCGAG
    CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC CCCAAACAGA CGGACTCCCA ACAGACAGAA
    AGCCCAGCCA AAGAGATCCC ACGAGCCACT GTGGCCCCGC TGGCTCTTGT CAAACCCAGA
    CCTGGAACGG TTTCACCAAT GGCCTCTCCG TTAGCCACAC CGGCTTTCAA AGACTCCATT
    CCACCCTGTA GTCCAGGCCC GAAGACTGGC AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG
    TCCCCCGCCA GCAGGCCTGC TTCCTTCACC TTCCCCGGGG ACGCCTGCGA CACGCTGAGC
    CGACCAAATC ACAACCAGAG TCAGCGGCAC TCCACCCACA GTAAAGACGC AGACCGTATG
    AGCACCTGCT CCTCTACCAG TGAGCAGTCT ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA
    AGCAGCAGCA GCAGTAATAT CTCCACCATG TTGGTGACTC AGGACTATGT GGCTCTGAAA
    GAGGATGAGA TTAATGTGGG TCAGGGGGAG GTGGTGCAGA TCCTGGCCTC CAATCAGCAG
    AATATGTTCT TGGTGTTTCG TGCCGCTACT GAGCAGTGTC CTGCCGCCGA GGGCTGGATC
    CCCGGATACG TGCTGGGACA CACATCCGCC ATCATCCCTG ACGCTCCAGA CGGAACCATA
    AACAGGAAAT CGTCATCATC ATGGCACACA TCCCTCCGTA TTCGAAAGAA GTCAGAGAAA
    AGAGAAAAGG ACAATAAAAA AGAGGCCAAG CTGGAAAACG GCTACCGGAA ATCCAGAGAG
    GGACTGACCA ACAAGGTCTC TGTGAAGCTT CTAAATCCCA ATTATATATA TGATGTTCTC
    CCGGAGTTCC TCGTCCCGCT GAGTGACGTG ACCTGTGATA AAGGCGAGTG TGTGACTCTG
    AGGTGTAAAG TGTGTGGTCG GCCCAAAGCC ACGGTCACGT GGAAGGGACC TGAGCAGAAA
    ACACTCACCA ACAACGGCCA CTTCAGCATC GCCTACAGTG AGACTGGTGA GGCCACGCTG
    CGGATCGTAG GTGTGACCTC AGAGGATGAT GGCAGTTACA CCTGCATTGC CACGAATGAC
    ATTGGCAGTG TGGCATCATC AGCCAGTCTC AGAGTGCTGG GCACGACCAG CGATGGGATC
    CGGGTCATGT GGAAAGACAA CTTTGAGTCC TTCTACACTG AGGTAGCAGA GCTTGGCAGG
    GGACGGTTCT CTGTAGTGAA ACGCTGTGAC CAGAGGGGAT CAAAGCGGAC CGTGGCGGTC
    AAGTTTGTCA ATAAGAAACT GATGAAGCGA GACCAGGTGA CCCATGAGTT CAGTGTCCTA
    CAGAGACTCC AGCACCCTCA TCTGGTCAGA CTGCTGGACA CCTTCGAGAC CTCCAGCAGC
    TACGCACTGG TCCTGGAGAT GTCTGATCAG GGCCGTCTGC TGGACTACAT CGTGAGCTGG
    GGGAATCTCA CTGAGGAGAA AGTGGCCTTT TACCTCCGGG ACATTTTAGA AGCTTTGCAG
    TACCTGCACA ACTGCAGAAT AGTCCATCTG GATCTAAAGC CTGAGAATCT GGTGGTGGAG
    CAGAGCCCGT CTCAGCCGCT GGTCAAGCTG ACGGACTTCG GTGATGCTGT GCAGCTCAAC
    TCCACGCCGT ACGTTCACCC TCTGCTGGGG AGCCCGGAGT TCGCAGCCCC TGAGCTGGTC
    CTCGGGGACC CTGTCTCTCT GTCCTCCGAC CTGTGGAGTC TGGGGGTCCT GACGTACGTC
    ATGCTGAGCG GAGCATCTCC GTTCCTTGAT GAGAGCGTGG AGGAGACCTG CCTCAACATC
    TGCCGCTTGG ACTTCAGCTT TCCTGATGAC TACTTCCAGG GAGTGAGTCA GGCGGCGCGT
    GATTTTATGT GCCTGCTGCT CCGGATGGAG CCATCCAAAC GTCCACCAGC CACCTCATGT
    CTACAGGAGC CCTGGTTGAG AGCCGGCGGG GGCCGACGCT CAGCCGAATG CATCGATACC
    TCCAGACTGA TCTCCTTCAT CGACCGACGC AAACACCAGA ACGACCTGCG GCCTCTCACT
    GGCATCAGAG CCTTCCTGCA GACTCGATTA CAGCCCAGGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292690.1
    CDS190..9474
    Translation

    Target ORF information:

    RefSeq Version XM_009294415.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294415.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGACGTC TTCACGTCTT TCCGTCAGAC CCTCCAGTTC TGAGACCCCC
    AGCGCTGCAG AGCTCGTCAG TGCCATTGAG GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA
    GACAGACCCA GCACTCTGTC AGTGGAGCAG GGCGACAGCA GCAGCCCTTC ATTCAACCCA
    TCTGATAACT CTCTCCTGTC CTCCTCCTCT CCAATCGATG AGATAGAGGA ACGCAAGACC
    GGTTTCTTCA AGAAGCGCCA TTACGTCCTG CTGGAGCTGA TCGAGACGGA GAGAGACTAT
    GTGCGAGATC TCAGTTTGGT CGTGGAGGGC TACATGGCCA GGATGAGGGA GGACGGGGTT
    CCAGATGATA TGAAAGGAAA AGACAAGATC GTTTTTGGAA ACATTCAGCA GATATATGAC
    TGGCACAAAG ATTTCTTCCT GGGAGAGCTT GAGAAGTGCC TTGAGGACCC TGACCGGCTG
    GGCCCTCTCT TCCTCAAACA TGAGCGGAGA CTGCACATGT ACATTGTTTA CTGCCAGAAC
    AAACCTAAAT CTGAACACAT CGTCTCAGAG TACATCGACA CATATTTTGA GGATCTGAAG
    CAGCGTTTGG GTCACAGGCT TCAGATCACT GATCTGCTCA TTAAACCTGT ACAGAGAATC
    ATGAAGTACC AGCTGCTGCT GAAGGATTTC CTCAAATTTT CCAAAAAAAT CGGAACAGAT
    TCGATTGAAT TAGAGAAAGC GGTTGAGGTC ATGTGCATTG TACCAAAAAG ATGCAACGAC
    ATGATGAATG TTGGTCGACT CCAGGGGTTT GATGGAAAGA TTGTTGCTCA GGGGCGTCTC
    CTACTCCAGG ACACCTTCAT GGTGGCCGAA CCAGAGGGAG GCCTGCTGAA CAGGATGAAG
    GAGAGGAGAG TCTTCCTCTT TGAGCAGATC GTCATCTTCA GTGAACCTCT AGATAAAAAG
    AGGGGCTTCT CCATGCCTGG CTATTTGTAC AAATACAGCA TCAAGGTGAA CTGTCTGGGA
    CTTGAGGACA GTGTGGACGG AGACCCCTGT AAATTTGCAC TGACCTCCAG AACGTCCAAT
    AGCAGCAAAG ATGCCTTCAT TCTGCACTCG AGCCATCCTG GAGTACGACA AGTCTGGATG
    CTGCAGATCA GCCAAATCCT GGAGAGCCAA CGCAATTTCC TCAATGCACT TACATCTCCT
    ATAGACTACC AGAGGAACCA CGTGGAGGGT CCTGGTGTGT CAGGCAGCGG TGTACAGGCT
    GGAGGCAGCG GGGGGCAGAT GATGGCCCCT GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG
    GGTTCCCGTT CTCGTCCGTC CCGTATCCCC CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT
    CATCACTCTC CTGCCCTGGG GCCTGGAGCC CACGAGCATG ACGGCCCCGA CAAACTATCA
    GGTATGTCCC CAAGACCTCT CTCCAGGGGT CCCTCACCCT CATGCACCAC AGAGCCCGAG
    CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC CCCAAACAGA CGGACTCCCA ACAGACAGAA
    AGCCCAGCCA AAGAGATCCC ACGAGCCACT GTGGCCCCGC TGGCTCTTGT CAAACCCAGA
    CCTGGAACGG TTTCACCAAT GGCCTCTCCG TTAGCCACAC CGGCTTTCAA AGACTCCATT
    CCACCCTGTA GTCCAGGCCC GAAGACTGGC AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG
    TCCCCCGCCA GCAGGCCTGC TTCCTTCACC TTCCCCGGGG ACGCCTGCGA CACGCTGAGC
    CGACCAAATC ACAACCAGAG TCAGCGGCAC TCCACCCACA GTAAAGACGC AGACCGTATG
    AGCACCTGCT CCTCTACCAG TGAGCAGTCT ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA
    AGCAGCAGCA GCAGTAATAT CTCCACCATG TTGGTGACTC AGGACTATGT GGCTCTGAAA
    GAGGATGAGA TTAATGTGGG TCAGGGGGAG GTGGTGCAGA TCCTGGCCTC CAATCAGCAG
    AATATGTTCT TGGTGTTTCG TGCCGCTACT GAGCAGTGTC CTGCCGCCGA GGGCTGGATC
    CCCGGATACG TGCTGGGACA CACATCCGCC ATCATCCCTG ACGCTCCAGA CGGAACCATA
    AACAGGAAAT CGTCATCATC ATGGCACACA TCCCTCCGTA TTCGAAAGAA GTCAGAGAAA
    AGAGAAAAGG ACAATAAAAA AGAGGCCAAG CTGGAAAACG GCTACCGGAA ATCCAGAGAG
    GGACTGACCA ACAAGGTCTC TGTGAAGCTT CTAAATCCCA ATTATATATA TGATGTTCTC
    CCGGAGTTCC TCGTCCCGCT GAGTGACGTG ACCTGTGATA AAGGCGAGTG TGTGACTCTG
    AGGTGTAAAG TGTGTGGTCG GCCCAAAGCC ACGGTCACGT GGAAGGGACC TGAGCAGAAA
    ACACTCACCA ACAACGGCCA CTTCAGCATC GCCTACAGTG AGACTGGTGA GGCCACGCTG
    CGGATCGTAG GTGTGACCTC AGAGGATGAT GGCAGTTACA CCTGCATTGC CACGAATGAC
    ATTGGCAGTG TGGCATCATC AGCCAGTCTC AGAGTGCTGG GCACGACCAG CGATGGGATC
    CGGGTCATGT GGAAAGACAA CTTTGAGTCC TTCTACACTG AGGTAGCAGA GCTTGGCAGG
    GGACGGTTCT CTGTAGTGAA ACGCTGTGAC CAGAGGGGAT CAAAGCGGAC CGTGGCGGTC
    AAGTTTGTCA ATAAGAAACT GATGAAGCGA GACCAGGTGA CCCATGAGTT CAGTGTCCTA
    CAGAGACTCC AGCACCCTCA TCTGGTCAGA CTGCTGGACA CCTTCGAGAC CTCCAGCAGC
    TACGCACTGG TCCTGGAGAT GTCTGATCAG GGCCGTCTGC TGGACTACAT CGTGAGCTGG
    GGGAATCTCA CTGAGGAGAA AGTGGCCTTT TACCTCCGGG ACATTTTAGA AGCTTTGCAG
    TACCTGCACA ACTGCAGAAT AGTCCATCTG GATCTAAAGC CTGAGAATCT GGTGGTGGAG
    CAGAGCCCGT CTCAGCCGCT GGTCAAGCTG ACGGACTTCG GTGATGCTGT GCAGCTCAAC
    TCCACGCCGT ACGTTCACCC TCTGCTGGGG AGCCCGGAGT TCGCAGCCCC TGAGCTGGTC
    CTCGGGGACC CTGTCTCTCT GTCCTCCGAC CTGTGGAGTC TGGGGGTCCT GACGTACGTC
    ATGCTGAGCG GAGCATCTCC GTTCCTTGAT GAGAGCGTGG AGGAGACCTG CCTCAACATC
    TGCCGCTTGG ACTTCAGCTT TCCTGATGAC TACTTCCAGG GAGTGAGTCA GGCGGCGCGT
    GATTTTATGT GCCTGCTGCT CCGGATGGAG CCATCCAAAC GTCCACCAGC CACCTCATGT
    CTACAGGAGC CCTGGTTGAG AGCCGGCGGG GGCCGACGCT CAGCCGAATG CATCGATACC
    TCCAGACTGA TCTCCTTCAT CGACCGACGC AAACACCAGA ACGACCTGCG GCCTCTCACT
    GGCATCAGAG CCTTCCTGCA GACTCGATTA CAGCCCAGGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42637
    Clone ID Related Accession (Same CDS sequence) XM_009294416.2
    Accession Version XM_009294416.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGACGTC TTCACGTCTT TCCGTCAGAC CCTCCAGTTC TGAGACCCCC
    AGCGCTGCAG AGCTCGTCAG TGCCATTGAG GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA
    GACAGACCCA GCACTCTGTC AGTGGAGCAG GGCGACAGCA GCAGCCCTTC ATTCAACCCA
    TCTGATAACT CTCTCCTGTC CTCCTCCTCT CCAATCGATG AGATAGAGGA ACGCAAGACC
    GGTTTCTTCA AGAAGCGCCA TTACGTCCTG CTGGAGCTGA TCGAGACGGA GAGAGACTAT
    GTGCGAGATC TCAGTTTGGT CGTGGAGGGC TACATGGCCA GGATGAGGGA GGACGGGGTT
    CCAGATGATA TGAAAGGAAA AGACAAGATC GTTTTTGGAA ACATTCAGCA GATATATGAC
    TGGCACAAAG ATTTCTTCCT GGGAGAGCTT GAGAAGTGCC TTGAGGACCC TGACCGGCTG
    GGCCCTCTCT TCCTCAAACA TGAGCGGAGA CTGCACATGT ACATTGTTTA CTGCCAGAAC
    AAACCTAAAT CTGAACACAT CGTCTCAGAG TACATCGACA CATATTTTGA GGATCTGAAG
    CAGCGTTTGG GTCACAGGCT TCAGATCACT GATCTGCTCA TTAAACCTGT ACAGAGAATC
    ATGAAGTACC AGCTGCTGCT GAAGGATTTC CTCAAATTTT CCAAAAAAAT CGGAACAGAT
    TCGATTGAAT TAGAGAAAGC GGTTGAGGTC ATGTGCATTG TACCAAAAAG ATGCAACGAC
    ATGATGAATG TTGGTCGACT CCAGGGGTTT GATGGAAAGA TTGTTGCTCA GGGGCGTCTC
    CTACTCCAGG ACACCTTCAT GGTGGCCGAA CCAGAGGGAG GCCTGCTGAA CAGGATGAAG
    GAGAGGAGAG TCTTCCTCTT TGAGCAGATC GTCATCTTCA GTGAACCTCT AGATAAAAAG
    AGGGGCTTCT CCATGCCTGG CTATTTGTAC AAATACAGCA TCAAGGTGAA CTGTCTGGGA
    CTTGAGGACA GTGTGGACGG AGACCCCTGT AAATTTGCAC TGACCTCCAG AACGTCCAAT
    AGCAGCAAAG ATGCCTTCAT TCTGCACTCG AGCCATCCTG GAGTACGACA AGTCTGGATG
    CTGCAGATCA GCCAAATCCT GGAGAGCCAA CGCAATTTCC TCAATGCACT TACATCTCCT
    ATAGACTACC AGAGGAACCA CGTGGAGGGT CCTGGTGTGT CAGGCAGCGG TGTACAGGCT
    GGAGGCAGCG GGGGGCAGAT GATGGCCCCT GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG
    GGTTCCCGTT CTCGTCCGTC CCGTATCCCC CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT
    CATCACTCTC CTGCCCTGGG GCCTGGAGCC CACGAGCATG ACGGCCCCGA CAAACTATCA
    GGTATGTCCC CAAGACCTCT CTCCAGGGGT CCCTCACCCT CATGCACCAC AGAGCCCGAG
    CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC CCCAAACAGA CGGACTCCCA ACAGACAGAA
    AGCCCAGCCA AAGAGATCCC ACGAGCCACT GTGGCCCCGC TGGCTCTTGT CAAACCCAGA
    CCTGGAACGG TTTCACCAAT GGCCTCTCCG TTAGCCACAC CGGCTTTCAA AGACTCCATT
    CCACCCTGTA GTCCAGGCCC GAAGACTGGC AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG
    TCCCCCGCCA GCAGGCCTGC TTCCTTCACC TTCCCCGGGG ACGCCTGCGA CACGCTGAGC
    CGACCAAATC ACAACCAGAG TCAGCGGCAC TCCACCCACA GTAAAGACGC AGACCGTATG
    AGCACCTGCT CCTCTACCAG TGAGCAGTCT ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA
    AGCAGCAGCA GCAGTAATAT CTCCACCATG TTGGTGACTC AGGACTATGT GGCTCTGAAA
    GAGGATGAGA TTAATGTGGG TCAGGGGGAG GTGGTGCAGA TCCTGGCCTC CAATCAGCAG
    AATATGTTCT TGGTGTTTCG TGCCGCTACT GAGCAGTGTC CTGCCGCCGA GGGCTGGATC
    CCCGGATACG TGCTGGGACA CACATCCGCC ATCATCCCTG ACGCTCCAGA CGGAACCATA
    AAGAAATCGT CATCATCATG GCACACATCC CTCCGTATTC GAAAGAAGTC AGAGAAAAGA
    GAAAAGGACA ATAAAAAAGA GGCCAAGCTG GAAAACGGCT ACCGGAAATC CAGAGAGGGA
    CTGACCAACA AGGTCTCTGT GAAGCTTCTA AATCCCAATT ATATATATGA TGTTCTCCCG
    GAGTTCCTCG TCCCGCTGAG TGACGTGACC TGTGATAAAG GCGAGTGTGT GACTCTGAGG
    TGTAAAGTGT GTGGTCGGCC CAAAGCCACG GTCACGTGGA AGGGACCTGA GCAGAAAACA
    CTCACCAACA ACGGCCACTT CAGCATCGCC TACAGTGAGA CTGGTGAGGC CACGCTGCGG
    ATCGTAGGTG TGACCTCAGA GGATGATGGC AGTTACACCT GCATTGCCAC GAATGACATT
    GGCAGTGTGG CATCATCAGC CAGTCTCAGA GTGCTGGGCA CGACCAGCGA TGGGATCCGG
    GTCATGTGGA AAGACAACTT TGAGTCCTTC TACACTGAGG TAGCAGAGCT TGGCAGGGGA
    CGGTTCTCTG TAGTGAAACG CTGTGACCAG AGGGGATCAA AGCGGACCGT GGCGGTCAAG
    TTTGTCAATA AGAAACTGAT GAAGCGAGAC CAGGTGACCC ATGAGTTCAG TGTCCTACAG
    AGACTCCAGC ACCCTCATCT GGTCAGACTG CTGGACACCT TCGAGACCTC CAGCAGCTAC
    GCACTGGTCC TGGAGATGTC TGATCAGGGC CGTCTGCTGG ACTACATCGT GAGCTGGGGG
    AATCTCACTG AGGAGAAAGT GGCCTTTTAC CTCCGGGACA TTTTAGAAGC TTTGCAGTAC
    CTGCACAACT GCAGAATAGT CCATCTGGAT CTAAAGCCTG AGAATCTGGT GGTGGAGCAG
    AGCCCGTCTC AGCCGCTGGT CAAGCTGACG GACTTCGGTG ATGCTGTGCA GCTCAACTCC
    ACGCCGTACG TTCACCCTCT GCTGGGGAGC CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC
    GGGGACCCTG TCTCTCTGTC CTCCGACCTG TGGAGTCTGG GGGTCCTGAC GTACGTCATG
    CTGAGCGGAG CATCTCCGTT CCTTGATGAG AGCGTGGAGG AGACCTGCCT CAACATCTGC
    CGCTTGGACT TCAGCTTTCC TGATGACTAC TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT
    TTTATGTGCC TGCTGCTCCG GATGGAGCCA TCCAAACGTC CACCAGCCAC CTCATGTCTA
    CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC CGACGCTCAG CCGAATGCAT CGATACCTCC
    AGACTGATCT CCTTCATCGA CCGACGCAAA CACCAGAACG ACCTGCGGCC TCTCACTGGC
    ATCAGAGCCT TCCTGCAGAC TCGATTACAG CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292691.1
    CDS191..9472
    Translation

    Target ORF information:

    RefSeq Version XM_009294416.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294416.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGCTAAGTCA GGGATGCAAA
    CATGGATCAT TGGCGTCCGG TATTGAGAAC AAACAGGACT GGATCAAGCA CATCCGGGAA
    GTGATTCAGG AGCGCACCGT TCACCTGAAG GGGGCGCTGA AAGAGCCCAT TCACATCCCC
    AAAGCCTCCA CAGCCAAACA CAAGGGGCGC AGGTACACCC CAAGGGATGG AGAGGATCTG
    GACAGTCAGG GTGACGGCAG CAGTCAACCA GACACCATCT CACTGGCCTC CAGGACCTCA
    CAGAACACAC TGGACAGCGA CAAGTTGTCT GGTGGCTGTG AGCTGACGGT GGTCATCCAT
    GACTTTATGG CCGGTAACAG TAATGAGCTG ACCATCCGGA GAGGACAGAC GGTGGAGGTG
    CTCGAGAGAC TTCATGATAA GCCGGACTGG TGTTTGGTTC GCACCACAGA CCGTTCGCCG
    GCGCTGGAGG GCCTGGTGCC CTGTGCCATG CTCTGTATCG CCCACTCCCG CAGCAGCATG
    GAGATGGAGG GCATCTTCAA CCACAAAGAT GCACTCTCTG TATGCAGTAA TGATGCCATC
    ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG GGTCATGTGA TGGGTTCCCA GAGTTCTCCT
    GGACCTAAAA GGCCTGGCAA CACATTAAGG AAATGGCTGA CCAGTCCTGT GAGGAGACTG
    AGCAGCGGTA AAGCTGACGG ACACGTCAAG AAGCTGGCAC ACAAACACAA GAAGAGCCGT
    GACGTCAGAA AGAGCGCAGA CGCCGGTTCA CAGAAAGACT CGGACGACAG CGCTGCGACG
    CCACAAGATG AGACCCTCGA GGAGAGGGTG CGTAATGAGG GTCTGAGCAG CGGCACGCTC
    TCCAAATCCT CCTCTTCAGG GATGCAGAGC TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT
    GACGCTGTGC CTCTTCCTCC ACCAATGGCC ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT
    GCGCAGGATG ACAAGACGTC TTCACGTCTT TCCGTCAGAC CCTCCAGTTC TGAGACCCCC
    AGCGCTGCAG AGCTCGTCAG TGCCATTGAG GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA
    GACAGACCCA GCACTCTGTC AGTGGAGCAG GGCGACAGCA GCAGCCCTTC ATTCAACCCA
    TCTGATAACT CTCTCCTGTC CTCCTCCTCT CCAATCGATG AGATAGAGGA ACGCAAGACC
    GGTTTCTTCA AGAAGCGCCA TTACGTCCTG CTGGAGCTGA TCGAGACGGA GAGAGACTAT
    GTGCGAGATC TCAGTTTGGT CGTGGAGGGC TACATGGCCA GGATGAGGGA GGACGGGGTT
    CCAGATGATA TGAAAGGAAA AGACAAGATC GTTTTTGGAA ACATTCAGCA GATATATGAC
    TGGCACAAAG ATTTCTTCCT GGGAGAGCTT GAGAAGTGCC TTGAGGACCC TGACCGGCTG
    GGCCCTCTCT TCCTCAAACA TGAGCGGAGA CTGCACATGT ACATTGTTTA CTGCCAGAAC
    AAACCTAAAT CTGAACACAT CGTCTCAGAG TACATCGACA CATATTTTGA GGATCTGAAG
    CAGCGTTTGG GTCACAGGCT TCAGATCACT GATCTGCTCA TTAAACCTGT ACAGAGAATC
    ATGAAGTACC AGCTGCTGCT GAAGGATTTC CTCAAATTTT CCAAAAAAAT CGGAACAGAT
    TCGATTGAAT TAGAGAAAGC GGTTGAGGTC ATGTGCATTG TACCAAAAAG ATGCAACGAC
    ATGATGAATG TTGGTCGACT CCAGGGGTTT GATGGAAAGA TTGTTGCTCA GGGGCGTCTC
    CTACTCCAGG ACACCTTCAT GGTGGCCGAA CCAGAGGGAG GCCTGCTGAA CAGGATGAAG
    GAGAGGAGAG TCTTCCTCTT TGAGCAGATC GTCATCTTCA GTGAACCTCT AGATAAAAAG
    AGGGGCTTCT CCATGCCTGG CTATTTGTAC AAATACAGCA TCAAGGTGAA CTGTCTGGGA
    CTTGAGGACA GTGTGGACGG AGACCCCTGT AAATTTGCAC TGACCTCCAG AACGTCCAAT
    AGCAGCAAAG ATGCCTTCAT TCTGCACTCG AGCCATCCTG GAGTACGACA AGTCTGGATG
    CTGCAGATCA GCCAAATCCT GGAGAGCCAA CGCAATTTCC TCAATGCACT TACATCTCCT
    ATAGACTACC AGAGGAACCA CGTGGAGGGT CCTGGTGTGT CAGGCAGCGG TGTACAGGCT
    GGAGGCAGCG GGGGGCAGAT GATGGCCCCT GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG
    GGTTCCCGTT CTCGTCCGTC CCGTATCCCC CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT
    CATCACTCTC CTGCCCTGGG GCCTGGAGCC CACGAGCATG ACGGCCCCGA CAAACTATCA
    GGTATGTCCC CAAGACCTCT CTCCAGGGGT CCCTCACCCT CATGCACCAC AGAGCCCGAG
    CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC CCCAAACAGA CGGACTCCCA ACAGACAGAA
    AGCCCAGCCA AAGAGATCCC ACGAGCCACT GTGGCCCCGC TGGCTCTTGT CAAACCCAGA
    CCTGGAACGG TTTCACCAAT GGCCTCTCCG TTAGCCACAC CGGCTTTCAA AGACTCCATT
    CCACCCTGTA GTCCAGGCCC GAAGACTGGC AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG
    TCCCCCGCCA GCAGGCCTGC TTCCTTCACC TTCCCCGGGG ACGCCTGCGA CACGCTGAGC
    CGACCAAATC ACAACCAGAG TCAGCGGCAC TCCACCCACA GTAAAGACGC AGACCGTATG
    AGCACCTGCT CCTCTACCAG TGAGCAGTCT ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA
    AGCAGCAGCA GCAGTAATAT CTCCACCATG TTGGTGACTC AGGACTATGT GGCTCTGAAA
    GAGGATGAGA TTAATGTGGG TCAGGGGGAG GTGGTGCAGA TCCTGGCCTC CAATCAGCAG
    AATATGTTCT TGGTGTTTCG TGCCGCTACT GAGCAGTGTC CTGCCGCCGA GGGCTGGATC
    CCCGGATACG TGCTGGGACA CACATCCGCC ATCATCCCTG ACGCTCCAGA CGGAACCATA
    AAGAAATCGT CATCATCATG GCACACATCC CTCCGTATTC GAAAGAAGTC AGAGAAAAGA
    GAAAAGGACA ATAAAAAAGA GGCCAAGCTG GAAAACGGCT ACCGGAAATC CAGAGAGGGA
    CTGACCAACA AGGTCTCTGT GAAGCTTCTA AATCCCAATT ATATATATGA TGTTCTCCCG
    GAGTTCCTCG TCCCGCTGAG TGACGTGACC TGTGATAAAG GCGAGTGTGT GACTCTGAGG
    TGTAAAGTGT GTGGTCGGCC CAAAGCCACG GTCACGTGGA AGGGACCTGA GCAGAAAACA
    CTCACCAACA ACGGCCACTT CAGCATCGCC TACAGTGAGA CTGGTGAGGC CACGCTGCGG
    ATCGTAGGTG TGACCTCAGA GGATGATGGC AGTTACACCT GCATTGCCAC GAATGACATT
    GGCAGTGTGG CATCATCAGC CAGTCTCAGA GTGCTGGGCA CGACCAGCGA TGGGATCCGG
    GTCATGTGGA AAGACAACTT TGAGTCCTTC TACACTGAGG TAGCAGAGCT TGGCAGGGGA
    CGGTTCTCTG TAGTGAAACG CTGTGACCAG AGGGGATCAA AGCGGACCGT GGCGGTCAAG
    TTTGTCAATA AGAAACTGAT GAAGCGAGAC CAGGTGACCC ATGAGTTCAG TGTCCTACAG
    AGACTCCAGC ACCCTCATCT GGTCAGACTG CTGGACACCT TCGAGACCTC CAGCAGCTAC
    GCACTGGTCC TGGAGATGTC TGATCAGGGC CGTCTGCTGG ACTACATCGT GAGCTGGGGG
    AATCTCACTG AGGAGAAAGT GGCCTTTTAC CTCCGGGACA TTTTAGAAGC TTTGCAGTAC
    CTGCACAACT GCAGAATAGT CCATCTGGAT CTAAAGCCTG AGAATCTGGT GGTGGAGCAG
    AGCCCGTCTC AGCCGCTGGT CAAGCTGACG GACTTCGGTG ATGCTGTGCA GCTCAACTCC
    ACGCCGTACG TTCACCCTCT GCTGGGGAGC CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC
    GGGGACCCTG TCTCTCTGTC CTCCGACCTG TGGAGTCTGG GGGTCCTGAC GTACGTCATG
    CTGAGCGGAG CATCTCCGTT CCTTGATGAG AGCGTGGAGG AGACCTGCCT CAACATCTGC
    CGCTTGGACT TCAGCTTTCC TGATGACTAC TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT
    TTTATGTGCC TGCTGCTCCG GATGGAGCCA TCCAAACGTC CACCAGCCAC CTCATGTCTA
    CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC CGACGCTCAG CCGAATGCAT CGATACCTCC
    AGACTGATCT CCTTCATCGA CCGACGCAAA CACCAGAACG ACCTGCGGCC TCTCACTGGC
    ATCAGAGCCT TCCTGCAGAC TCGATTACAG CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42638
    Clone ID Related Accession (Same CDS sequence) XM_009294417.2
    Accession Version XM_009294417.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9241
    ATGAGCTCGG GGGAAGGGGC CGAGGAGGCG GCTAAGGACG CGGCCGATAT AGCGACATTC 
    TTCAAATCTG ATTGTGCGCT TAAATATGAG CCCAAAGCTG ACGTCTCCAA TCAGATAACA
    GGATTTCGCA GGAATGAAGA AATGAAGGCG ATGGAGGTGT TGCCCATTTT AAAAGAAAAA
    GTGGCCTTCT TATCAGGGGG CAGAGACAAG CGTGGCGGTC CAGTTTTAAC ATTCCCATCA
    CGAACCAACC ATGACCGAAT ACGACACGAG GATCTCCGCA GACTCATCGC CTACCTCGCC
    GGCATCCCAA GCGAGGATGT CTGCAAACAT GGCTTCACGG TCATCGTGGA CATGCGTGGC
    TCCAAATGGG ATTCCATCAA GCCTCTGCTG AAGATCCTCC AGGAGTCGTT CCCCTGCTGC
    ATCCACATCG CCCTCATCAT CAAACCAGAC AACTTCTGGC AGAAACAGAG GACCAATTTC
    GGCAGCTCCA AGTTTGAGTT TGAGACCACA ATGGTTTCTT TGGAGGGCTT ATCGAAGGTG
    GTGGACCCAT CCCAGTTGAC GGCTGACTTT GAGGGCAGCC TGGACTACAA CCATGAAGAG
    TGGATTGAAA TCCGCGTGGC CTTCGAAGAC TTTACAGGCA ATGCTCGGCA CCTGCTCGCC
    CGGCTAGAAG AGATGCATGA AACGGTGACG CGTAAAGACT TACCGCAGGA CTTAGATGGA
    GCCCGACGGA TGATAGAGGA GCACGCGGCT TTGAAGAAGA GGGTGATAAA GGCTCCGGTG
    GAGGAGGTGG ACAATGAGGG CCAAAGGCTC CTGCAAAGGA TCCAGAGCAG TGAGAGCTAC
    GCCAACCGAA CTGTGCCTGT GCCGCCTGGT CAGAGGGAAG GGCAGGGGCA GCCCAACGCC
    GACACCCAGG GCCTGGTGCC CCGTATTACA GCTCTGCTGG AGAAACTGCA CAGCACCAGG
    CAGAACCTGC ACCAGTCCTG GCACATCCGC AAGCTGCAGC TGGACCAGTG CTTCCAGCTC
    AGGCTGTTCG AGCAGGACGC TGAGAAGATG TTTGATTGGA TCATGCACAA TAAAGGGCTG
    TTTCTGGCTG GATACACTGA GATCGGCAAC AACCACCCTC ACGCCATGGA GCTGCAGACC
    CAGCACAATC ACTTCGCCAT GAACTGCATG AATGTTTACG TGAACATAAA CCGCATCATG
    TCTGTGGGGA ACCGGCTGCT GGAGGCCGGT CATTACGCGT CGCAGCAGAT CAAGCAGATC
    TCAGGTCAGC TGGAGCAGGA GTGGAAAGCT TTCGCCGCTG CGCTGGACGA GAGGAGTGCT
    CTGCTGGAGA TGTCTGCTAC CTTCCACCAG AAATGCGACC AGTACATGAG TAATGTGGAC
    TCGTGGTGTA AAGCGTGTGG AGAAGTGGAT CTGCCCTCAG AGCTGCAGGA ACTGGAGGAC
    GCTATACACC ATCACCAGGG CCTGTATGAA CACATCACCG CTGCCTACTC GGAGGTGAGC
    CAAGACGGAA AAGCCTTATT GGACAAACTT CAGCGCCCCT TGACGCCCGG CAGTGCTGAT
    TCGCTGACAG CTTCCGCCAA TTACACCAAG GCGGTGAACC ATGTTTTGGA CATCATCCAT
    GAGGTTCTGC ATCACCAAAG GCAGCTGGAG AACATCTGGC AACATCGCAA GGTCCGGCTG
    CACCAGCGTC TGCAGCTGTG TGTTTTCCAG CAGGACGTTC AGCAGGTTCT GGACTGGATT
    GAGAACCATG GCGAGGCATT TCTCAGCAAG CACACAGGGG TGGGAAAATC TCTGCATCGC
    GCTCGAGCTC TGCAGAAACG CCATGAAGAC TTTGAGGAAG TGGCTCAGAA CACGTACACA
    AATGCGGATA AGCTTCTGGA AGCGGCAGAG CAGCTGGCCC AGACAGGCGA GTGTGACCCA
    GAGGAGATCT ATCAGGCCGC TCATCAGCTG GAGGACCGAA TCCAGGACTT TGTTCGGCGC
    GTGGAGCAGC GCAAGGTCCT GCTGGACATG TCAGTGGCTT TCCACACTCA CGTCAAAGAG
    TTGTGGACGT GGCTGGAGGA GCTGCAGAAG GAGTTGCTGG ACGATGTGTA CGCCGAGTCT
    GTGGAAGCCG TGCAGGATCT GATCAAGCGT TTCGGGCAGC AGCAACAAAC CACACTGCAG
    TTCACTGTAA ACGTCATCAA AGAAGGAGAA GATCTCATCC AGCAGCTCAG AGACCTGGCC
    ATCTCCAGCA ACAAGACGCC TCACAACAGC TCCATCAACC ACATCGAGAG CGTCCTGCAG
    CAGCTGGATG AGGCTCAGGC TCAGATGGAG GAGCTTTTCC AGGAGAGGAA GATCAAGCTG
    GAGCTTTTCC TGCAGCTCAG GATCTTTGAG CGGGACGCCA TAGATATTAT CTCAGATCTG
    GACTCGTGGA ATGAGGAGCT GTCGCAGCAG ATGAATGAGT TTGATACAGA AGACCTGACG
    CTGGCTGAAC AGAGACTGCA GCACCATGCT GATAAAGCCC TGACCATGAA CAACCTGGCC
    TTCCACGTCA TCCACCAGGG CCAGGAACTG CTGCAGTACG TCAACGAGGT GCAGGCGTCT
    GGTGTGGAGT TGCTGTGTGA CCGTGATGTG GACATGGCCA CCCGTGTTCA GGACCTGCTA
    GAGTTTCTAC ATGAGAAACA GCAAGAGCTG GACGTGGCTG CAGAACAGCA TCGCAGACAC
    CTTGAGCAGT GTGTGCAACT GCGCCACCTA CAAGCCGAAG TCAAACAGGT TCTGGGTTGG
    ATCCGTAATG GCGAGTCGAT GCTGAATGCA GGTCTGATTA CGGCCAGCTC TCTTCAAGAA
    GCAGAGCAGT TGCAGAGAGA GCATGAGCAA TTCCAGCATG CTATCGAGAA AACCCATCAG
    AGCGCCCTGC AGGTCCAGCA GAAAGCCGAA GCCCTGCTGC AGGCCAATCA CTACGACATG
    GACATGATTC GAGACTGCGC GGAGAACGTG GCTTCCCACT GGCAGCAGCT CATGCTGAAG
    ATGGAGGACC GGCTCAAGCT GGTCAACGCC TCCGTGGCCT TCTATAAGAC CTCTGAACAG
    GTTTGCAGTG TTCTGGAGAG TCTGGAGCAG GAGTACAAGC GTGAGGAGGA CTGGTGCGGA
    GGAGCTGACA AACTGGGACC CAACTGTGAA TCGGACCACG TCACACCCAT GATCAGCAAA
    CACCTGGAGC AGAAAGAGGC TTTCCTCAAG GCCTGCACGT TGGCCAGGCG TAATGCAGAC
    GTCTTCCTCA AATACATGCA CAGGAACAGC GTCAGCATGC CCGGGATGTT GAGTCACGTC
    AAAGCTCCAG AGCAGCAGGT CAAAAATATC CTGAATGAGC TGCTGCAGAG GGAGAACCGC
    GTGCTGCACT TCTGGACTAT GAGAAAGAGG AGGCTGGACC AGTGTCAGCA ATACGTGGTG
    TTTGAACGCA GCGCCAAACA GGCTCTGGAG TGGATCCACG ACACTGGCGA GTTTTATCTG
    TCCACACACA CGTCCACCGG CTCCAGCATA CACCACACAC AGGAGCTGCT GAAGGAGCAC
    GAGGACTTCC ACATCACAGC CAAGCAAACC AAAGAACGGG TGAAGCTGCT GATCCAGCTG
    GCAGATGGTT TCTGTGATAA GGGTCACTCT CACGCCGCTG AGATTAAGAA ATGGGTGACA
    GCGGTGGACA AACGCTATCG AGATTTCTCG CTCCGCATGG ACAAGTACCG CACATCTCTG
    GAGAAAGCAC TCGGCATCTC CTCAGACTCC AATAAAGCTA GCAAAGACTT GCAGCTGGAC
    ATCATCCCTG CCAGTGCTCC GGGGTCAGAG GTCAAACTCA GAGACGCTGC TCATGAGCTC
    AATGAAGAGA AGCGCAAGTC TGCCCGCAGG AAGGACTTCA TCATGGCTGA GCTGATCCAG
    ACAGAAAAGG CCTATGTGCG AGACTTGAGA GAGTGCATGG ACACGTATCT GTGGGAGATG
    ACCAGCGGCG TGGAGGAGAT CCCACCAGGC ATCGTCAACA AAGAGCACAT CATTTTTGGT
    AACATGCAAG ATCTGTACGA GTTCCATCAC AATATATTCC TCAAAGAGCT GGAGAAATAC
    GAGCAGCTTC CTGAAGATGT GGGCCACTGT TTTGTGACTT GGGCTGATAA GTTCCAGATG
    TATGTGAACT ACTGCAAGAA CAAACCAGAC TCCACTCAGC TTATTCTTGA ACATGCCGGA
    GGATACTTCG ACGAGATCCA GCAGAGGCAT CGCCTGGCCA ACTCCATCTC CTCATACCTC
    ATCAAACCTG TTCAGAGAAT AACCAAGTAC CAGCTGCTCC TCAAGGAGCT CCTGACGTGC
    TGCGAGGAAG GCAAAGGTGA AATTAAAGAT GGCCTGGAGG TGATGCTCAG TGTTCCCAAA
    AGAGCCAACG ACGCCATGCA CTTGAGCATG CTTGAAGGGT TTGATGAGAA CATCGAGTCC
    CAGGGAGAGC TGATCCTGCA GGAGGCGTTT CAGGTGTGGG ATCCCAAAAC TCTGATCCGC
    AAGGGAAGAG AACGACACCT GTTCCTCTTC GAGATGTCAC TGATCTTCAG CAAGGAGGTC
    AAAGACTCCA ATGGCAGGAG CAAGTACATC TACAAGAGCA AGCTTTTCAC CTCTGAACTG
    GGTGTCACTG AGCATGTGGA GGGAGATCCA TGCAAATTTG CTCTCTGGGT TGGACGAACC
    CCAACTTCTG ACAACAAGAT TGTCCTCAAG CTAAGTCAGG GATGCAAACA TGGATCATTG
    GCGTCCGGTA TTGAGAACAA ACAGGACTGG ATCAAGCACA TCCGGGAAGT GATTCAGGAG
    CGCACCGTTC ACCTGAAGGG GGCGCTGAAA GAGCCCATTC ACATCCCCAA AGCCTCCACA
    GCCAAACACA AGGGGCGCAG GTACACCCCA AGGGATGGAG AGGATCTGGA CAGTCAGGGT
    GACGGCAGCA GTCAACCAGA CACCATCTCA CTGGCCTCCA GGACCTCACA GAACACACTG
    GACAGCGACA AGTTGTCTGG TGGCTGTGAG CTGACGGTGG TCATCCATGA CTTTATGGCC
    GGTAACAGTA ATGAGCTGAC CATCCGGAGA GGACAGACGG TGGAGGTGCT CGAGAGACTT
    CATGATAAGC CGGACTGGTG TTTGGTTCGC ACCACAGACC GTTCGCCGGC GCTGGAGGGC
    CTGGTGCCCT GTGCCATGCT CTGTATCGCC CACTCCCGCA GCAGCATGGA GATGGAGGGC
    ATCTTCAACC ACAAAGATGC ACTCTCTGTA TGCAGTAATG ATGCCATCAT GCCAGGCTCC
    TCTGCCACCC TGCAGCCGGG TCATGTGATG GGTTCCCAGA GTTCTCCTGG ACCTAAAAGG
    CCTGGCAACA CATTAAGGAA ATGGCTGACC AGTCCTGTGA GGAGACTGAG CAGCGGTAAA
    GCTGACGGAC ACGTCAAGAA GCTGGCACAC AAACACAAGA AGAGCCGTGA CGTCAGAAAG
    AGCGCAGACG CCGGTTCACA GAAAGACTCG GACGACAGCG CTGCGACGCC ACAAGATGAG
    ACCCTCGAGG AGAGGGTGCG TAATGAGGGT CTGAGCAGCG GCACGCTCTC CAAATCCTCC
    TCTTCAGGGA TGCAGAGCTG TGGAGAGGAA GAGGGAGAGG AAGGAGCTGA CGCTGTGCCT
    CTTCCTCCAC CAATGGCCAT CCAGCAGCAC AGCCTCCTCC AGCCGGATGC GCAGGATGAC
    AAGACGTCTT CACGTCTTTC CGTCAGACCC TCCAGTTCTG AGACCCCCAG CGCTGCAGAG
    CTCGTCAGTG CCATTGAGGA GCTGGTCAAA AGCAAGATGG CTCTGGAAGA CAGACCCAGC
    ACTCTGTCAG TGGAGCAGGG CGACAGCAGC AGCCCTTCAT TCAACCCATC TGATAACTCT
    CTCCTGTCCT CCTCCTCTCC AATCGATGAG ATAGAGGAAC GCAAGACCGG TTTCTTCAAG
    AAGCGCCATT ACGTCCTGCT GGAGCTGATC GAGACGGAGA GAGACTATGT GCGAGATCTC
    AGTTTGGTCG TGGAGGGCTA CATGGCCAGG ATGAGGGAGG ACGGGGTTCC AGATGATATG
    AAAGGAAAAG ACAAGATCGT TTTTGGAAAC ATTCAGCAGA TATATGACTG GCACAAAGAT
    TTCTTCCTGG GAGAGCTTGA GAAGTGCCTT GAGGACCCTG ACCGGCTGGG CCCTCTCTTC
    CTCAAACATG AGCGGAGACT GCACATGTAC ATTGTTTACT GCCAGAACAA ACCTAAATCT
    GAACACATCG TCTCAGAGTA CATCGACACA TATTTTGAGG ATCTGAAGCA GCGTTTGGGT
    CACAGGCTTC AGATCACTGA TCTGCTCATT AAACCTGTAC AGAGAATCAT GAAGTACCAG
    CTGCTGCTGA AGGATTTCCT CAAATTTTCC AAAAAAATCG GAACAGATTC GATTGAATTA
    GAGAAAGCGG TTGAGGTCAT GTGCATTGTA CCAAAAAGAT GCAACGACAT GATGAATGTT
    GGTCGACTCC AGGGGTTTGA TGGAAAGATT GTTGCTCAGG GGCGTCTCCT ACTCCAGGAC
    ACCTTCATGG TGGCCGAACC AGAGGGAGGC CTGCTGAACA GGATGAAGGA GAGGAGAGTC
    TTCCTCTTTG AGCAGATCGT CATCTTCAGT GAACCTCTAG ATAAAAAGAG GGGCTTCTCC
    ATGCCTGGCT ATTTGTACAA ATACAGCATC AAGGTGAACT GTCTGGGACT TGAGGACAGT
    GTGGACGGAG ACCCCTGTAA ATTTGCACTG ACCTCCAGAA CGTCCAATAG CAGCAAAGAT
    GCCTTCATTC TGCACTCGAG CCATCCTGGA GTACGACAAG TCTGGATGCT GCAGATCAGC
    CAAATCCTGG AGAGCCAACG CAATTTCCTC AATGCACTTA CATCTCCTAT AGACTACCAG
    AGGAACCACG TGGAGGGTCC TGGTGTGTCA GGCAGCGGTG TACAGGCTGG AGGCAGCGGG
    GGGCAGATGA TGGCCCCTGG AGGAGGAGTA GGGGTCCCGG CTGGGCCGGG TTCCCGTTCT
    CGTCCGTCCC GTATCCCCCA GCCCTCGCGC TTGCCTCAAC CGCTCCGTCA TCACTCTCCT
    GCCCTGGGGC CTGGAGCCCA CGAGCATGAC GGCCCCGACA AACTATCAGG TATGTCCCCA
    AGACCTCTCT CCAGGGGTCC CTCACCCTCA TGCACCACAG AGCCCGAGCC AAAGGTGAAG
    CTTCCTGCGA GCCCTCACCC CAAACAGACG GACTCCCAAC AGACAGAAAG CCCAGCCAAA
    GAGATCCCAC GAGCCACTGT GGCCCCGCTG GCTCTTGTCA AACCCAGACC TGGAACGGTT
    TCACCAATGG CCTCTCCGTT AGCCACACCG GCTTTCAAAG ACTCCATTCC ACCCTGTAGT
    CCAGGCCCGA AGACTGGCAG CAGCTCGTTT TGGAGCTCAG TGCCCGCGTC CCCCGCCAGC
    AGGCCTGCTT CCTTCACCTT CCCCGGGGAC GCCTGCGACA CGCTGAGCCG ACCAAATCAC
    AACCAGAGTC AGCGGCACTC CACCCACAGT AAAGACGCAG ACCGTATGAG CACCTGCTCC
    TCTACCAGTG AGCAGTCTAT CCAGTCCACT CAGAGCAATG GGAGTGAAAG CAGCAGCAGC
    AGTAATATCT CCACCATGTT GGTGACTCAG GACTATGTGG CTCTGAAAGA GGATGAGATT
    AATGTGGGTC AGGGGGAGGT GGTGCAGATC CTGGCCTCCA ATCAGCAGAA TATGTTCTTG
    GTGTTTCGTG CCGCTACTGA GCAGTGTCCT GCCGCCGAGG GCTGGATCCC CGGATACGTG
    CTGGGACACA CATCCGCCAT CATCCCTGAC GCTCCAGACG GAACCATAAA CAGGAAATCG
    TCATCATCAT GGCACACATC CCTCCGTATT CGAAAGAAGT CAGAGAAAAG AGAAAAGGAC
    AATAAAAAAG AGGCCAAGCT GGAAAACGGC TACCGGAAAT CCAGAGAGGG ACTGACCAAC
    AAGGTCTCTG TGAAGCTTCT AAATCCCAAT TATATATATG ATGTTCTCCC GGAGTTCCTC
    GTCCCGCTGA GTGACGTGAC CTGTGATAAA GGCGAGTGTG TGACTCTGAG GTGTAAAGTG
    TGTGGTCGGC CCAAAGCCAC GGTCACGTGG AAGGGACCTG AGCAGAAAAC ACTCACCAAC
    AACGGCCACT TCAGCATCGC CTACAGTGAG ACTGGTGAGG CCACGCTGCG GATCGTAGGT
    GTGACCTCAG AGGATGATGG CAGTTACACC TGCATTGCCA CGAATGACAT TGGCAGTGTG
    GCATCATCAG CCAGTCTCAG AGTGCTGGGC ACGACCAGCG ATGGGATCCG GGTCATGTGG
    AAAGACAACT TTGAGTCCTT CTACACTGAG GTAGCAGAGC TTGGCAGGGG ACGGTTCTCT
    GTAGTGAAAC GCTGTGACCA GAGGGGATCA AAGCGGACCG TGGCGGTCAA GTTTGTCAAT
    AAGAAACTGA TGAAGCGAGA CCAGGTGACC CATGAGTTCA GTGTCCTACA GAGACTCCAG
    CACCCTCATC TGGTCAGACT GCTGGACACC TTCGAGACCT CCAGCAGCTA CGCACTGGTC
    CTGGAGATGT CTGATCAGGG CCGTCTGCTG GACTACATCG TGAGCTGGGG GAATCTCACT
    GAGGAGAAAG TGGCCTTTTA CCTCCGGGAC ATTTTAGAAG CTTTGCAGTA CCTGCACAAC
    TGCAGAATAG TCCATCTGGA TCTAAAGCCT GAGAATCTGG TGGTGGAGCA GAGCCCGTCT
    CAGCCGCTGG TCAAGCTGAC GGACTTCGGT GATGCTGTGC AGCTCAACTC CACGCCGTAC
    GTTCACCCTC TGCTGGGGAG CCCGGAGTTC GCAGCCCCTG AGCTGGTCCT CGGGGACCCT
    GTCTCTCTGT CCTCCGACCT GTGGAGTCTG GGGGTCCTGA CGTACGTCAT GCTGAGCGGA
    GCATCTCCGT TCCTTGATGA GAGCGTGGAG GAGACCTGCC TCAACATCTG CCGCTTGGAC
    TTCAGCTTTC CTGATGACTA CTTCCAGGGA GTGAGTCAGG CGGCGCGTGA TTTTATGTGC
    CTGCTGCTCC GGATGGAGCC ATCCAAACGT CCACCAGCCA CCTCATGTCT ACAGGAGCCC
    TGGTTGAGAG CCGGCGGGGG CCGACGCTCA GCCGAATGCA TCGATACCTC CAGACTGATC
    TCCTTCATCG ACCGACGCAA ACACCAGAAC GACCTGCGGC CTCTCACTGG CATCAGAGCC
    TTCCTGCAGA CTCGATTACA GCCCAGGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292692.1
    CDS228..9500
    Translation

    Target ORF information:

    RefSeq Version XM_009294417.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294417.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGAGCTCGG GGGAAGGGGC CGAGGAGGCG GCTAAGGACG CGGCCGATAT AGCGACATTC 
    TTCAAATCTG ATTGTGCGCT TAAATATGAG CCCAAAGCTG ACGTCTCCAA TCAGATAACA
    GGATTTCGCA GGAATGAAGA AATGAAGGCG ATGGAGGTGT TGCCCATTTT AAAAGAAAAA
    GTGGCCTTCT TATCAGGGGG CAGAGACAAG CGTGGCGGTC CAGTTTTAAC ATTCCCATCA
    CGAACCAACC ATGACCGAAT ACGACACGAG GATCTCCGCA GACTCATCGC CTACCTCGCC
    GGCATCCCAA GCGAGGATGT CTGCAAACAT GGCTTCACGG TCATCGTGGA CATGCGTGGC
    TCCAAATGGG ATTCCATCAA GCCTCTGCTG AAGATCCTCC AGGAGTCGTT CCCCTGCTGC
    ATCCACATCG CCCTCATCAT CAAACCAGAC AACTTCTGGC AGAAACAGAG GACCAATTTC
    GGCAGCTCCA AGTTTGAGTT TGAGACCACA ATGGTTTCTT TGGAGGGCTT ATCGAAGGTG
    GTGGACCCAT CCCAGTTGAC GGCTGACTTT GAGGGCAGCC TGGACTACAA CCATGAAGAG
    TGGATTGAAA TCCGCGTGGC CTTCGAAGAC TTTACAGGCA ATGCTCGGCA CCTGCTCGCC
    CGGCTAGAAG AGATGCATGA AACGGTGACG CGTAAAGACT TACCGCAGGA CTTAGATGGA
    GCCCGACGGA TGATAGAGGA GCACGCGGCT TTGAAGAAGA GGGTGATAAA GGCTCCGGTG
    GAGGAGGTGG ACAATGAGGG CCAAAGGCTC CTGCAAAGGA TCCAGAGCAG TGAGAGCTAC
    GCCAACCGAA CTGTGCCTGT GCCGCCTGGT CAGAGGGAAG GGCAGGGGCA GCCCAACGCC
    GACACCCAGG GCCTGGTGCC CCGTATTACA GCTCTGCTGG AGAAACTGCA CAGCACCAGG
    CAGAACCTGC ACCAGTCCTG GCACATCCGC AAGCTGCAGC TGGACCAGTG CTTCCAGCTC
    AGGCTGTTCG AGCAGGACGC TGAGAAGATG TTTGATTGGA TCATGCACAA TAAAGGGCTG
    TTTCTGGCTG GATACACTGA GATCGGCAAC AACCACCCTC ACGCCATGGA GCTGCAGACC
    CAGCACAATC ACTTCGCCAT GAACTGCATG AATGTTTACG TGAACATAAA CCGCATCATG
    TCTGTGGGGA ACCGGCTGCT GGAGGCCGGT CATTACGCGT CGCAGCAGAT CAAGCAGATC
    TCAGGTCAGC TGGAGCAGGA GTGGAAAGCT TTCGCCGCTG CGCTGGACGA GAGGAGTGCT
    CTGCTGGAGA TGTCTGCTAC CTTCCACCAG AAATGCGACC AGTACATGAG TAATGTGGAC
    TCGTGGTGTA AAGCGTGTGG AGAAGTGGAT CTGCCCTCAG AGCTGCAGGA ACTGGAGGAC
    GCTATACACC ATCACCAGGG CCTGTATGAA CACATCACCG CTGCCTACTC GGAGGTGAGC
    CAAGACGGAA AAGCCTTATT GGACAAACTT CAGCGCCCCT TGACGCCCGG CAGTGCTGAT
    TCGCTGACAG CTTCCGCCAA TTACACCAAG GCGGTGAACC ATGTTTTGGA CATCATCCAT
    GAGGTTCTGC ATCACCAAAG GCAGCTGGAG AACATCTGGC AACATCGCAA GGTCCGGCTG
    CACCAGCGTC TGCAGCTGTG TGTTTTCCAG CAGGACGTTC AGCAGGTTCT GGACTGGATT
    GAGAACCATG GCGAGGCATT TCTCAGCAAG CACACAGGGG TGGGAAAATC TCTGCATCGC
    GCTCGAGCTC TGCAGAAACG CCATGAAGAC TTTGAGGAAG TGGCTCAGAA CACGTACACA
    AATGCGGATA AGCTTCTGGA AGCGGCAGAG CAGCTGGCCC AGACAGGCGA GTGTGACCCA
    GAGGAGATCT ATCAGGCCGC TCATCAGCTG GAGGACCGAA TCCAGGACTT TGTTCGGCGC
    GTGGAGCAGC GCAAGGTCCT GCTGGACATG TCAGTGGCTT TCCACACTCA CGTCAAAGAG
    TTGTGGACGT GGCTGGAGGA GCTGCAGAAG GAGTTGCTGG ACGATGTGTA CGCCGAGTCT
    GTGGAAGCCG TGCAGGATCT GATCAAGCGT TTCGGGCAGC AGCAACAAAC CACACTGCAG
    TTCACTGTAA ACGTCATCAA AGAAGGAGAA GATCTCATCC AGCAGCTCAG AGACCTGGCC
    ATCTCCAGCA ACAAGACGCC TCACAACAGC TCCATCAACC ACATCGAGAG CGTCCTGCAG
    CAGCTGGATG AGGCTCAGGC TCAGATGGAG GAGCTTTTCC AGGAGAGGAA GATCAAGCTG
    GAGCTTTTCC TGCAGCTCAG GATCTTTGAG CGGGACGCCA TAGATATTAT CTCAGATCTG
    GACTCGTGGA ATGAGGAGCT GTCGCAGCAG ATGAATGAGT TTGATACAGA AGACCTGACG
    CTGGCTGAAC AGAGACTGCA GCACCATGCT GATAAAGCCC TGACCATGAA CAACCTGGCC
    TTCCACGTCA TCCACCAGGG CCAGGAACTG CTGCAGTACG TCAACGAGGT GCAGGCGTCT
    GGTGTGGAGT TGCTGTGTGA CCGTGATGTG GACATGGCCA CCCGTGTTCA GGACCTGCTA
    GAGTTTCTAC ATGAGAAACA GCAAGAGCTG GACGTGGCTG CAGAACAGCA TCGCAGACAC
    CTTGAGCAGT GTGTGCAACT GCGCCACCTA CAAGCCGAAG TCAAACAGGT TCTGGGTTGG
    ATCCGTAATG GCGAGTCGAT GCTGAATGCA GGTCTGATTA CGGCCAGCTC TCTTCAAGAA
    GCAGAGCAGT TGCAGAGAGA GCATGAGCAA TTCCAGCATG CTATCGAGAA AACCCATCAG
    AGCGCCCTGC AGGTCCAGCA GAAAGCCGAA GCCCTGCTGC AGGCCAATCA CTACGACATG
    GACATGATTC GAGACTGCGC GGAGAACGTG GCTTCCCACT GGCAGCAGCT CATGCTGAAG
    ATGGAGGACC GGCTCAAGCT GGTCAACGCC TCCGTGGCCT TCTATAAGAC CTCTGAACAG
    GTTTGCAGTG TTCTGGAGAG TCTGGAGCAG GAGTACAAGC GTGAGGAGGA CTGGTGCGGA
    GGAGCTGACA AACTGGGACC CAACTGTGAA TCGGACCACG TCACACCCAT GATCAGCAAA
    CACCTGGAGC AGAAAGAGGC TTTCCTCAAG GCCTGCACGT TGGCCAGGCG TAATGCAGAC
    GTCTTCCTCA AATACATGCA CAGGAACAGC GTCAGCATGC CCGGGATGTT GAGTCACGTC
    AAAGCTCCAG AGCAGCAGGT CAAAAATATC CTGAATGAGC TGCTGCAGAG GGAGAACCGC
    GTGCTGCACT TCTGGACTAT GAGAAAGAGG AGGCTGGACC AGTGTCAGCA ATACGTGGTG
    TTTGAACGCA GCGCCAAACA GGCTCTGGAG TGGATCCACG ACACTGGCGA GTTTTATCTG
    TCCACACACA CGTCCACCGG CTCCAGCATA CACCACACAC AGGAGCTGCT GAAGGAGCAC
    GAGGACTTCC ACATCACAGC CAAGCAAACC AAAGAACGGG TGAAGCTGCT GATCCAGCTG
    GCAGATGGTT TCTGTGATAA GGGTCACTCT CACGCCGCTG AGATTAAGAA ATGGGTGACA
    GCGGTGGACA AACGCTATCG AGATTTCTCG CTCCGCATGG ACAAGTACCG CACATCTCTG
    GAGAAAGCAC TCGGCATCTC CTCAGACTCC AATAAAGCTA GCAAAGACTT GCAGCTGGAC
    ATCATCCCTG CCAGTGCTCC GGGGTCAGAG GTCAAACTCA GAGACGCTGC TCATGAGCTC
    AATGAAGAGA AGCGCAAGTC TGCCCGCAGG AAGGACTTCA TCATGGCTGA GCTGATCCAG
    ACAGAAAAGG CCTATGTGCG AGACTTGAGA GAGTGCATGG ACACGTATCT GTGGGAGATG
    ACCAGCGGCG TGGAGGAGAT CCCACCAGGC ATCGTCAACA AAGAGCACAT CATTTTTGGT
    AACATGCAAG ATCTGTACGA GTTCCATCAC AATATATTCC TCAAAGAGCT GGAGAAATAC
    GAGCAGCTTC CTGAAGATGT GGGCCACTGT TTTGTGACTT GGGCTGATAA GTTCCAGATG
    TATGTGAACT ACTGCAAGAA CAAACCAGAC TCCACTCAGC TTATTCTTGA ACATGCCGGA
    GGATACTTCG ACGAGATCCA GCAGAGGCAT CGCCTGGCCA ACTCCATCTC CTCATACCTC
    ATCAAACCTG TTCAGAGAAT AACCAAGTAC CAGCTGCTCC TCAAGGAGCT CCTGACGTGC
    TGCGAGGAAG GCAAAGGTGA AATTAAAGAT GGCCTGGAGG TGATGCTCAG TGTTCCCAAA
    AGAGCCAACG ACGCCATGCA CTTGAGCATG CTTGAAGGGT TTGATGAGAA CATCGAGTCC
    CAGGGAGAGC TGATCCTGCA GGAGGCGTTT CAGGTGTGGG ATCCCAAAAC TCTGATCCGC
    AAGGGAAGAG AACGACACCT GTTCCTCTTC GAGATGTCAC TGATCTTCAG CAAGGAGGTC
    AAAGACTCCA ATGGCAGGAG CAAGTACATC TACAAGAGCA AGCTTTTCAC CTCTGAACTG
    GGTGTCACTG AGCATGTGGA GGGAGATCCA TGCAAATTTG CTCTCTGGGT TGGACGAACC
    CCAACTTCTG ACAACAAGAT TGTCCTCAAG CTAAGTCAGG GATGCAAACA TGGATCATTG
    GCGTCCGGTA TTGAGAACAA ACAGGACTGG ATCAAGCACA TCCGGGAAGT GATTCAGGAG
    CGCACCGTTC ACCTGAAGGG GGCGCTGAAA GAGCCCATTC ACATCCCCAA AGCCTCCACA
    GCCAAACACA AGGGGCGCAG GTACACCCCA AGGGATGGAG AGGATCTGGA CAGTCAGGGT
    GACGGCAGCA GTCAACCAGA CACCATCTCA CTGGCCTCCA GGACCTCACA GAACACACTG
    GACAGCGACA AGTTGTCTGG TGGCTGTGAG CTGACGGTGG TCATCCATGA CTTTATGGCC
    GGTAACAGTA ATGAGCTGAC CATCCGGAGA GGACAGACGG TGGAGGTGCT CGAGAGACTT
    CATGATAAGC CGGACTGGTG TTTGGTTCGC ACCACAGACC GTTCGCCGGC GCTGGAGGGC
    CTGGTGCCCT GTGCCATGCT CTGTATCGCC CACTCCCGCA GCAGCATGGA GATGGAGGGC
    ATCTTCAACC ACAAAGATGC ACTCTCTGTA TGCAGTAATG ATGCCATCAT GCCAGGCTCC
    TCTGCCACCC TGCAGCCGGG TCATGTGATG GGTTCCCAGA GTTCTCCTGG ACCTAAAAGG
    CCTGGCAACA CATTAAGGAA ATGGCTGACC AGTCCTGTGA GGAGACTGAG CAGCGGTAAA
    GCTGACGGAC ACGTCAAGAA GCTGGCACAC AAACACAAGA AGAGCCGTGA CGTCAGAAAG
    AGCGCAGACG CCGGTTCACA GAAAGACTCG GACGACAGCG CTGCGACGCC ACAAGATGAG
    ACCCTCGAGG AGAGGGTGCG TAATGAGGGT CTGAGCAGCG GCACGCTCTC CAAATCCTCC
    TCTTCAGGGA TGCAGAGCTG TGGAGAGGAA GAGGGAGAGG AAGGAGCTGA CGCTGTGCCT
    CTTCCTCCAC CAATGGCCAT CCAGCAGCAC AGCCTCCTCC AGCCGGATGC GCAGGATGAC
    AAGACGTCTT CACGTCTTTC CGTCAGACCC TCCAGTTCTG AGACCCCCAG CGCTGCAGAG
    CTCGTCAGTG CCATTGAGGA GCTGGTCAAA AGCAAGATGG CTCTGGAAGA CAGACCCAGC
    ACTCTGTCAG TGGAGCAGGG CGACAGCAGC AGCCCTTCAT TCAACCCATC TGATAACTCT
    CTCCTGTCCT CCTCCTCTCC AATCGATGAG ATAGAGGAAC GCAAGACCGG TTTCTTCAAG
    AAGCGCCATT ACGTCCTGCT GGAGCTGATC GAGACGGAGA GAGACTATGT GCGAGATCTC
    AGTTTGGTCG TGGAGGGCTA CATGGCCAGG ATGAGGGAGG ACGGGGTTCC AGATGATATG
    AAAGGAAAAG ACAAGATCGT TTTTGGAAAC ATTCAGCAGA TATATGACTG GCACAAAGAT
    TTCTTCCTGG GAGAGCTTGA GAAGTGCCTT GAGGACCCTG ACCGGCTGGG CCCTCTCTTC
    CTCAAACATG AGCGGAGACT GCACATGTAC ATTGTTTACT GCCAGAACAA ACCTAAATCT
    GAACACATCG TCTCAGAGTA CATCGACACA TATTTTGAGG ATCTGAAGCA GCGTTTGGGT
    CACAGGCTTC AGATCACTGA TCTGCTCATT AAACCTGTAC AGAGAATCAT GAAGTACCAG
    CTGCTGCTGA AGGATTTCCT CAAATTTTCC AAAAAAATCG GAACAGATTC GATTGAATTA
    GAGAAAGCGG TTGAGGTCAT GTGCATTGTA CCAAAAAGAT GCAACGACAT GATGAATGTT
    GGTCGACTCC AGGGGTTTGA TGGAAAGATT GTTGCTCAGG GGCGTCTCCT ACTCCAGGAC
    ACCTTCATGG TGGCCGAACC AGAGGGAGGC CTGCTGAACA GGATGAAGGA GAGGAGAGTC
    TTCCTCTTTG AGCAGATCGT CATCTTCAGT GAACCTCTAG ATAAAAAGAG GGGCTTCTCC
    ATGCCTGGCT ATTTGTACAA ATACAGCATC AAGGTGAACT GTCTGGGACT TGAGGACAGT
    GTGGACGGAG ACCCCTGTAA ATTTGCACTG ACCTCCAGAA CGTCCAATAG CAGCAAAGAT
    GCCTTCATTC TGCACTCGAG CCATCCTGGA GTACGACAAG TCTGGATGCT GCAGATCAGC
    CAAATCCTGG AGAGCCAACG CAATTTCCTC AATGCACTTA CATCTCCTAT AGACTACCAG
    AGGAACCACG TGGAGGGTCC TGGTGTGTCA GGCAGCGGTG TACAGGCTGG AGGCAGCGGG
    GGGCAGATGA TGGCCCCTGG AGGAGGAGTA GGGGTCCCGG CTGGGCCGGG TTCCCGTTCT
    CGTCCGTCCC GTATCCCCCA GCCCTCGCGC TTGCCTCAAC CGCTCCGTCA TCACTCTCCT
    GCCCTGGGGC CTGGAGCCCA CGAGCATGAC GGCCCCGACA AACTATCAGG TATGTCCCCA
    AGACCTCTCT CCAGGGGTCC CTCACCCTCA TGCACCACAG AGCCCGAGCC AAAGGTGAAG
    CTTCCTGCGA GCCCTCACCC CAAACAGACG GACTCCCAAC AGACAGAAAG CCCAGCCAAA
    GAGATCCCAC GAGCCACTGT GGCCCCGCTG GCTCTTGTCA AACCCAGACC TGGAACGGTT
    TCACCAATGG CCTCTCCGTT AGCCACACCG GCTTTCAAAG ACTCCATTCC ACCCTGTAGT
    CCAGGCCCGA AGACTGGCAG CAGCTCGTTT TGGAGCTCAG TGCCCGCGTC CCCCGCCAGC
    AGGCCTGCTT CCTTCACCTT CCCCGGGGAC GCCTGCGACA CGCTGAGCCG ACCAAATCAC
    AACCAGAGTC AGCGGCACTC CACCCACAGT AAAGACGCAG ACCGTATGAG CACCTGCTCC
    TCTACCAGTG AGCAGTCTAT CCAGTCCACT CAGAGCAATG GGAGTGAAAG CAGCAGCAGC
    AGTAATATCT CCACCATGTT GGTGACTCAG GACTATGTGG CTCTGAAAGA GGATGAGATT
    AATGTGGGTC AGGGGGAGGT GGTGCAGATC CTGGCCTCCA ATCAGCAGAA TATGTTCTTG
    GTGTTTCGTG CCGCTACTGA GCAGTGTCCT GCCGCCGAGG GCTGGATCCC CGGATACGTG
    CTGGGACACA CATCCGCCAT CATCCCTGAC GCTCCAGACG GAACCATAAA CAGGAAATCG
    TCATCATCAT GGCACACATC CCTCCGTATT CGAAAGAAGT CAGAGAAAAG AGAAAAGGAC
    AATAAAAAAG AGGCCAAGCT GGAAAACGGC TACCGGAAAT CCAGAGAGGG ACTGACCAAC
    AAGGTCTCTG TGAAGCTTCT AAATCCCAAT TATATATATG ATGTTCTCCC GGAGTTCCTC
    GTCCCGCTGA GTGACGTGAC CTGTGATAAA GGCGAGTGTG TGACTCTGAG GTGTAAAGTG
    TGTGGTCGGC CCAAAGCCAC GGTCACGTGG AAGGGACCTG AGCAGAAAAC ACTCACCAAC
    AACGGCCACT TCAGCATCGC CTACAGTGAG ACTGGTGAGG CCACGCTGCG GATCGTAGGT
    GTGACCTCAG AGGATGATGG CAGTTACACC TGCATTGCCA CGAATGACAT TGGCAGTGTG
    GCATCATCAG CCAGTCTCAG AGTGCTGGGC ACGACCAGCG ATGGGATCCG GGTCATGTGG
    AAAGACAACT TTGAGTCCTT CTACACTGAG GTAGCAGAGC TTGGCAGGGG ACGGTTCTCT
    GTAGTGAAAC GCTGTGACCA GAGGGGATCA AAGCGGACCG TGGCGGTCAA GTTTGTCAAT
    AAGAAACTGA TGAAGCGAGA CCAGGTGACC CATGAGTTCA GTGTCCTACA GAGACTCCAG
    CACCCTCATC TGGTCAGACT GCTGGACACC TTCGAGACCT CCAGCAGCTA CGCACTGGTC
    CTGGAGATGT CTGATCAGGG CCGTCTGCTG GACTACATCG TGAGCTGGGG GAATCTCACT
    GAGGAGAAAG TGGCCTTTTA CCTCCGGGAC ATTTTAGAAG CTTTGCAGTA CCTGCACAAC
    TGCAGAATAG TCCATCTGGA TCTAAAGCCT GAGAATCTGG TGGTGGAGCA GAGCCCGTCT
    CAGCCGCTGG TCAAGCTGAC GGACTTCGGT GATGCTGTGC AGCTCAACTC CACGCCGTAC
    GTTCACCCTC TGCTGGGGAG CCCGGAGTTC GCAGCCCCTG AGCTGGTCCT CGGGGACCCT
    GTCTCTCTGT CCTCCGACCT GTGGAGTCTG GGGGTCCTGA CGTACGTCAT GCTGAGCGGA
    GCATCTCCGT TCCTTGATGA GAGCGTGGAG GAGACCTGCC TCAACATCTG CCGCTTGGAC
    TTCAGCTTTC CTGATGACTA CTTCCAGGGA GTGAGTCAGG CGGCGCGTGA TTTTATGTGC
    CTGCTGCTCC GGATGGAGCC ATCCAAACGT CCACCAGCCA CCTCATGTCT ACAGGAGCCC
    TGGTTGAGAG CCGGCGGGGG CCGACGCTCA GCCGAATGCA TCGATACCTC CAGACTGATC
    TCCTTCATCG ACCGACGCAA ACACCAGAAC GACCTGCGGC CTCTCACTGG CATCAGAGCC
    TTCCTGCAGA CTCGATTACA GCCCAGGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42639
    Clone ID Related Accession (Same CDS sequence) XM_009294418.2
    Accession Version XM_009294418.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGACGTC TTCACGTCTT
    TCCGTCAGAC CCTCCAGTTC TGAGACCCCC AGCGCTGCAG AGCTCGTCAG TGCCATTGAG
    GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA GACAGACCCA GCACTCTGTC AGTGGAGCAG
    GGCGACAGCA GCAGCCCTTC ATTCAACCCA TCTGATAACT CTCTCCTGTC CTCCTCCTCT
    CCAATCGATG AGATAGAGGA ACGCAAGACC GGTTTCTTCA AGAAGCGCCA TTACGTCCTG
    CTGGAGCTGA TCGAGACGGA GAGAGACTAT GTGCGAGATC TCAGTTTGGT CGTGGAGGGC
    TACATGGCCA GGATGAGGGA GGACGGGGTT CCAGATGATA TGAAAGGAAA AGACAAGATC
    GTTTTTGGAA ACATTCAGCA GATATATGAC TGGCACAAAG ATTTCTTCCT GGGAGAGCTT
    GAGAAGTGCC TTGAGGACCC TGACCGGCTG GGCCCTCTCT TCCTCAAACA TGAGCGGAGA
    CTGCACATGT ACATTGTTTA CTGCCAGAAC AAACCTAAAT CTGAACACAT CGTCTCAGAG
    TACATCGACA CATATTTTGA GGATCTGAAG CAGCGTTTGG GTCACAGGCT TCAGATCACT
    GATCTGCTCA TTAAACCTGT ACAGAGAATC ATGAAGTACC AGCTGCTGCT GAAGGATTTC
    CTCAAATTTT CCAAAAAAAT CGGAACAGAT TCGATTGAAT TAGAGAAAGC GGTTGAGGTC
    ATGTGCATTG TACCAAAAAG ATGCAACGAC ATGATGAATG TTGGTCGACT CCAGGGGTTT
    GATGGAAAGA TTGTTGCTCA GGGGCGTCTC CTACTCCAGG ACACCTTCAT GGTGGCCGAA
    CCAGAGGGAG GCCTGCTGAA CAGGATGAAG GAGAGGAGAG TCTTCCTCTT TGAGCAGATC
    GTCATCTTCA GTGAACCTCT AGATAAAAAG AGGGGCTTCT CCATGCCTGG CTATTTGTAC
    AAATACAGCA TCAAGGTGAA CTGTCTGGGA CTTGAGGACA GTGTGGACGG AGACCCCTGT
    AAATTTGCAC TGACCTCCAG AACGTCCAAT AGCAGCAAAG ATGCCTTCAT TCTGCACTCG
    AGCCATCCTG GAGTACGACA AGTCTGGATG CTGCAGATCA GCCAAATCCT GGAGAGCCAA
    CGCAATTTCC TCAATGCACT TACATCTCCT ATAGACTACC AGAGGAACCA CGTGGAGGGT
    CCTGGTGTGT CAGGCAGCGG TGTACAGGCT GGAGGCAGCG GGGGGCAGAT GATGGCCCCT
    GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG GGTTCCCGTT CTCGTCCGTC CCGTATCCCC
    CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT CATCACTCTC CTGCCCTGGG GCCTGGAGCC
    CACGAGCATG ACGGCCCCGA CAAACTATCA GGTATGTCCC CAAGACCTCT CTCCAGGGGT
    CCCTCACCCT CATGCACCAC AGAGCCCGAG CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC
    CCCAAACAGA CGGACTCCCA ACAGACAGAA AGCCCAGCCA AAGAGATCCC ACGAGCCACT
    GTGGCCCCGC TGGCTCTTGT CAAACCCAGA CCTGGAACGG TTTCACCAAT GGCCTCTCCG
    TTAGCCACAC CGGCTTTCAA AGACTCCATT CCACCCTGTA GTCCAGGCCC GAAGACTGGC
    AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG TCCCCCGCCA GCAGGCCTGC TTCCTTCACC
    TTCCCCGGGG ACGCCTGCGA CACGCTGAGC CGACCAAATC ACAACCAGAG TCAGCGGCAC
    TCCACCCACA GTAAAGACGC AGACCGTATG AGCACCTGCT CCTCTACCAG TGAGCAGTCT
    ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA AGCAGCAGCA GCAGTAATAT CTCCACCATG
    TTGGTGACTC AGGACTATGT GGCTCTGAAA GAGGATGAGA TTAATGTGGG TCAGGGGGAG
    GTGGTGCAGA TCCTGGCCTC CAATCAGCAG AATATGTTCT TGGTGTTTCG TGCCGCTACT
    GAGCAGTGTC CTGCCGCCGA GGGCTGGATC CCCGGATACG TGCTGGGACA CACATCCGCC
    ATCATCCCTG ACGCTCCAGA CGGAACCATA AACAGGAAAT CGTCATCATC ATGGCACACA
    TCCCTCCGTA TTCGAAAGAA GTCAGAGAAA AGAGAAAAGG ACAATAAAAA AGAGGCCAAG
    CTGGAAAACG GCTACCGGAA ATCCAGAGAG GGACTGACCA ACAAGGTCTC TGTGAAGCTT
    CTAAATCCCA ATTATATATA TGATGTTCTC CCGGAGTTCC TCGTCCCGCT GAGTGACGTG
    ACCTGTGATA AAGGCGAGTG TGTGACTCTG AGGTGTAAAG TGTGTGGTCG GCCCAAAGCC
    ACGGTCACGT GGAAGGGACC TGAGCAGAAA ACACTCACCA ACAACGGCCA CTTCAGCATC
    GCCTACAGTG AGACTGGTGA GGCCACGCTG CGGATCGTAG GTGTGACCTC AGAGGATGAT
    GGCAGTTACA CCTGCATTGC CACGAATGAC ATTGGCAGTG TGGCATCATC AGCCAGTCTC
    AGAGTGCTGG GCACGACCAG CGATGGGATC CGGGTCATGT GGAAAGACAA CTTTGAGTCC
    TTCTACACTG AGGTAGCAGA GCTTGGCAGG GGACGGTTCT CTGTAGTGAA ACGCTGTGAC
    CAGAGGGGAT CAAAGCGGAC CGTGGCGGTC AAGTTTGTCA ATAAGAAACT GATGAAGCGA
    GACCAGGTGA CCCATGAGTT CAGTGTCCTA CAGAGACTCC AGCACCCTCA TCTGGTCAGA
    CTGCTGGACA CCTTCGAGAC CTCCAGCAGC TACGCACTGG TCCTGGAGAT GTCTGATCAG
    GGCCGTCTGC TGGACTACAT CGTGAGCTGG GGGAATCTCA CTGAGGAGAA AGTGGCCTTT
    TACCTCCGGG ACATTTTAGA AGCTTTGCAG TACCTGCACA ACTGCAGAAT AGTCCATCTG
    GATCTAAAGC CTGAGAATCT GGTGGTGGAG CAGAGCCCGT CTCAGCCGCT GGTCAAGCTG
    ACGGACTTCG GTGATGCTGT GCAGCTCAAC TCCACGCCGT ACGTTCACCC TCTGCTGGGG
    AGCCCGGAGT TCGCAGCCCC TGAGCTGGTC CTCGGGGACC CTGTCTCTCT GTCCTCCGAC
    CTGTGGAGTC TGGGGGTCCT GACGTACGTC ATGCTGAGCG GAGCATCTCC GTTCCTTGAT
    GAGAGCGTGG AGGAGACCTG CCTCAACATC TGCCGCTTGG ACTTCAGCTT TCCTGATGAC
    TACTTCCAGG GAGTGAGTCA GGCGGCGCGT GATTTTATGT GCCTGCTGCT CCGGATGGAG
    CCATCCAAAC GTCCACCAGC CACCTCATGT CTACAGGAGC CCTGGTTGAG AGCCGGCGGG
    GGCCGACGCT CAGCCGAATG CATCGATACC TCCAGACTGA TCTCCTTCAT CGACCGACGC
    AAACACCAGA ACGACCTGCG GCCTCTCACT GGCATCAGAG CCTTCCTGCA GACTCGATTA
    CAGCCCAGGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292693.1
    CDS190..9444
    Translation

    Target ORF information:

    RefSeq Version XM_009294418.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294418.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGACGTC TTCACGTCTT
    TCCGTCAGAC CCTCCAGTTC TGAGACCCCC AGCGCTGCAG AGCTCGTCAG TGCCATTGAG
    GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA GACAGACCCA GCACTCTGTC AGTGGAGCAG
    GGCGACAGCA GCAGCCCTTC ATTCAACCCA TCTGATAACT CTCTCCTGTC CTCCTCCTCT
    CCAATCGATG AGATAGAGGA ACGCAAGACC GGTTTCTTCA AGAAGCGCCA TTACGTCCTG
    CTGGAGCTGA TCGAGACGGA GAGAGACTAT GTGCGAGATC TCAGTTTGGT CGTGGAGGGC
    TACATGGCCA GGATGAGGGA GGACGGGGTT CCAGATGATA TGAAAGGAAA AGACAAGATC
    GTTTTTGGAA ACATTCAGCA GATATATGAC TGGCACAAAG ATTTCTTCCT GGGAGAGCTT
    GAGAAGTGCC TTGAGGACCC TGACCGGCTG GGCCCTCTCT TCCTCAAACA TGAGCGGAGA
    CTGCACATGT ACATTGTTTA CTGCCAGAAC AAACCTAAAT CTGAACACAT CGTCTCAGAG
    TACATCGACA CATATTTTGA GGATCTGAAG CAGCGTTTGG GTCACAGGCT TCAGATCACT
    GATCTGCTCA TTAAACCTGT ACAGAGAATC ATGAAGTACC AGCTGCTGCT GAAGGATTTC
    CTCAAATTTT CCAAAAAAAT CGGAACAGAT TCGATTGAAT TAGAGAAAGC GGTTGAGGTC
    ATGTGCATTG TACCAAAAAG ATGCAACGAC ATGATGAATG TTGGTCGACT CCAGGGGTTT
    GATGGAAAGA TTGTTGCTCA GGGGCGTCTC CTACTCCAGG ACACCTTCAT GGTGGCCGAA
    CCAGAGGGAG GCCTGCTGAA CAGGATGAAG GAGAGGAGAG TCTTCCTCTT TGAGCAGATC
    GTCATCTTCA GTGAACCTCT AGATAAAAAG AGGGGCTTCT CCATGCCTGG CTATTTGTAC
    AAATACAGCA TCAAGGTGAA CTGTCTGGGA CTTGAGGACA GTGTGGACGG AGACCCCTGT
    AAATTTGCAC TGACCTCCAG AACGTCCAAT AGCAGCAAAG ATGCCTTCAT TCTGCACTCG
    AGCCATCCTG GAGTACGACA AGTCTGGATG CTGCAGATCA GCCAAATCCT GGAGAGCCAA
    CGCAATTTCC TCAATGCACT TACATCTCCT ATAGACTACC AGAGGAACCA CGTGGAGGGT
    CCTGGTGTGT CAGGCAGCGG TGTACAGGCT GGAGGCAGCG GGGGGCAGAT GATGGCCCCT
    GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG GGTTCCCGTT CTCGTCCGTC CCGTATCCCC
    CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT CATCACTCTC CTGCCCTGGG GCCTGGAGCC
    CACGAGCATG ACGGCCCCGA CAAACTATCA GGTATGTCCC CAAGACCTCT CTCCAGGGGT
    CCCTCACCCT CATGCACCAC AGAGCCCGAG CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC
    CCCAAACAGA CGGACTCCCA ACAGACAGAA AGCCCAGCCA AAGAGATCCC ACGAGCCACT
    GTGGCCCCGC TGGCTCTTGT CAAACCCAGA CCTGGAACGG TTTCACCAAT GGCCTCTCCG
    TTAGCCACAC CGGCTTTCAA AGACTCCATT CCACCCTGTA GTCCAGGCCC GAAGACTGGC
    AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG TCCCCCGCCA GCAGGCCTGC TTCCTTCACC
    TTCCCCGGGG ACGCCTGCGA CACGCTGAGC CGACCAAATC ACAACCAGAG TCAGCGGCAC
    TCCACCCACA GTAAAGACGC AGACCGTATG AGCACCTGCT CCTCTACCAG TGAGCAGTCT
    ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA AGCAGCAGCA GCAGTAATAT CTCCACCATG
    TTGGTGACTC AGGACTATGT GGCTCTGAAA GAGGATGAGA TTAATGTGGG TCAGGGGGAG
    GTGGTGCAGA TCCTGGCCTC CAATCAGCAG AATATGTTCT TGGTGTTTCG TGCCGCTACT
    GAGCAGTGTC CTGCCGCCGA GGGCTGGATC CCCGGATACG TGCTGGGACA CACATCCGCC
    ATCATCCCTG ACGCTCCAGA CGGAACCATA AACAGGAAAT CGTCATCATC ATGGCACACA
    TCCCTCCGTA TTCGAAAGAA GTCAGAGAAA AGAGAAAAGG ACAATAAAAA AGAGGCCAAG
    CTGGAAAACG GCTACCGGAA ATCCAGAGAG GGACTGACCA ACAAGGTCTC TGTGAAGCTT
    CTAAATCCCA ATTATATATA TGATGTTCTC CCGGAGTTCC TCGTCCCGCT GAGTGACGTG
    ACCTGTGATA AAGGCGAGTG TGTGACTCTG AGGTGTAAAG TGTGTGGTCG GCCCAAAGCC
    ACGGTCACGT GGAAGGGACC TGAGCAGAAA ACACTCACCA ACAACGGCCA CTTCAGCATC
    GCCTACAGTG AGACTGGTGA GGCCACGCTG CGGATCGTAG GTGTGACCTC AGAGGATGAT
    GGCAGTTACA CCTGCATTGC CACGAATGAC ATTGGCAGTG TGGCATCATC AGCCAGTCTC
    AGAGTGCTGG GCACGACCAG CGATGGGATC CGGGTCATGT GGAAAGACAA CTTTGAGTCC
    TTCTACACTG AGGTAGCAGA GCTTGGCAGG GGACGGTTCT CTGTAGTGAA ACGCTGTGAC
    CAGAGGGGAT CAAAGCGGAC CGTGGCGGTC AAGTTTGTCA ATAAGAAACT GATGAAGCGA
    GACCAGGTGA CCCATGAGTT CAGTGTCCTA CAGAGACTCC AGCACCCTCA TCTGGTCAGA
    CTGCTGGACA CCTTCGAGAC CTCCAGCAGC TACGCACTGG TCCTGGAGAT GTCTGATCAG
    GGCCGTCTGC TGGACTACAT CGTGAGCTGG GGGAATCTCA CTGAGGAGAA AGTGGCCTTT
    TACCTCCGGG ACATTTTAGA AGCTTTGCAG TACCTGCACA ACTGCAGAAT AGTCCATCTG
    GATCTAAAGC CTGAGAATCT GGTGGTGGAG CAGAGCCCGT CTCAGCCGCT GGTCAAGCTG
    ACGGACTTCG GTGATGCTGT GCAGCTCAAC TCCACGCCGT ACGTTCACCC TCTGCTGGGG
    AGCCCGGAGT TCGCAGCCCC TGAGCTGGTC CTCGGGGACC CTGTCTCTCT GTCCTCCGAC
    CTGTGGAGTC TGGGGGTCCT GACGTACGTC ATGCTGAGCG GAGCATCTCC GTTCCTTGAT
    GAGAGCGTGG AGGAGACCTG CCTCAACATC TGCCGCTTGG ACTTCAGCTT TCCTGATGAC
    TACTTCCAGG GAGTGAGTCA GGCGGCGCGT GATTTTATGT GCCTGCTGCT CCGGATGGAG
    CCATCCAAAC GTCCACCAGC CACCTCATGT CTACAGGAGC CCTGGTTGAG AGCCGGCGGG
    GGCCGACGCT CAGCCGAATG CATCGATACC TCCAGACTGA TCTCCTTCAT CGACCGACGC
    AAACACCAGA ACGACCTGCG GCCTCTCACT GGCATCAGAG CCTTCCTGCA GACTCGATTA
    CAGCCCAGGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42640
    Clone ID Related Accession (Same CDS sequence) XM_009294419.2
    Accession Version XM_009294419.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGCGTGGCT CCAAATGGGA TTCCATCAAG CCTCTGCTGA AGATCCTCCA GGAGTCGTTC 
    CCCTGCTGCA TCCACATCGC CCTCATCATC AAACCAGACA ACTTCTGGCA GAAACAGAGG
    ACCAATTTCG GCAGCTCCAA GTTTGAGTTT GAGACCACAA TGGTTTCTTT GGAGGGCTTA
    TCGAAGGTGG TGGACCCATC CCAGTTGACG GCTGACTTTG AGGGCAGCCT GGACTACAAC
    CATGAAGAGT GGATTGAAAT CCGCGTGGCC TTCGAAGACT TTACAGGCAA TGCTCGGCAC
    CTGCTCGCCC GGCTAGAAGA GATGCATGAA ACGGTGACGC GTAAAGACTT ACCGCAGGAC
    TTAGATGGAG CCCGACGGAT GATAGAGGAG CACGCGGCTT TGAAGAAGAG GGTGATAAAG
    GCTCCGGTGG AGGAGGTGGA CAATGAGGGC CAAAGGCTCC TGCAAAGGAT CCAGAGCAGT
    GAGAGCTACG CCAACCGAAC TGTGCCTGTG CCGCCTGGTC AGAGGGAAGG GCAGGGGCAG
    CCCAACGCCG ACACCCAGGG CCTGGTGCCC CGTATTACAG CTCTGCTGGA GAAACTGCAC
    AGCACCAGGC AGAACCTGCA CCAGTCCTGG CACATCCGCA AGCTGCAGCT GGACCAGTGC
    TTCCAGCTCA GGCTGTTCGA GCAGGACGCT GAGAAGATGT TTGATTGGAT CATGCACAAT
    AAAGGGCTGT TTCTGGCTGG ATACACTGAG ATCGGCAACA ACCACCCTCA CGCCATGGAG
    CTGCAGACCC AGCACAATCA CTTCGCCATG AACTGCATGA ATGTTTACGT GAACATAAAC
    CGCATCATGT CTGTGGGGAA CCGGCTGCTG GAGGCCGGTC ATTACGCGTC GCAGCAGATC
    AAGCAGATCT CAGGTCAGCT GGAGCAGGAG TGGAAAGCTT TCGCCGCTGC GCTGGACGAG
    AGGAGTGCTC TGCTGGAGAT GTCTGCTACC TTCCACCAGA AATGCGACCA GTACATGAGT
    AATGTGGACT CGTGGTGTAA AGCGTGTGGA GAAGTGGATC TGCCCTCAGA GCTGCAGGAA
    CTGGAGGACG CTATACACCA TCACCAGGGC CTGTATGAAC ACATCACCGC TGCCTACTCG
    GAGGTGAGCC AAGACGGAAA AGCCTTATTG GACAAACTTC AGCGCCCCTT GACGCCCGGC
    AGTGCTGATT CGCTGACAGC TTCCGCCAAT TACACCAAGG CGGTGAACCA TGTTTTGGAC
    ATCATCCATG AGGTTCTGCA TCACCAAAGG CAGCTGGAGA ACATCTGGCA ACATCGCAAG
    GTCCGGCTGC ACCAGCGTCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTTCTG
    GACTGGATTG AGAACCATGG CGAGGCATTT CTCAGCAAGC ACACAGGGGT GGGAAAATCT
    CTGCATCGCG CTCGAGCTCT GCAGAAACGC CATGAAGACT TTGAGGAAGT GGCTCAGAAC
    ACGTACACAA ATGCGGATAA GCTTCTGGAA GCGGCAGAGC AGCTGGCCCA GACAGGCGAG
    TGTGACCCAG AGGAGATCTA TCAGGCCGCT CATCAGCTGG AGGACCGAAT CCAGGACTTT
    GTTCGGCGCG TGGAGCAGCG CAAGGTCCTG CTGGACATGT CAGTGGCTTT CCACACTCAC
    GTCAAAGAGT TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGTTGCTGGA CGATGTGTAC
    GCCGAGTCTG TGGAAGCCGT GCAGGATCTG ATCAAGCGTT TCGGGCAGCA GCAACAAACC
    ACACTGCAGT TCACTGTAAA CGTCATCAAA GAAGGAGAAG ATCTCATCCA GCAGCTCAGA
    GACCTGGCCA TCTCCAGCAA CAAGACGCCT CACAACAGCT CCATCAACCA CATCGAGAGC
    GTCCTGCAGC AGCTGGATGA GGCTCAGGCT CAGATGGAGG AGCTTTTCCA GGAGAGGAAG
    ATCAAGCTGG AGCTTTTCCT GCAGCTCAGG ATCTTTGAGC GGGACGCCAT AGATATTATC
    TCAGATCTGG ACTCGTGGAA TGAGGAGCTG TCGCAGCAGA TGAATGAGTT TGATACAGAA
    GACCTGACGC TGGCTGAACA GAGACTGCAG CACCATGCTG ATAAAGCCCT GACCATGAAC
    AACCTGGCCT TCCACGTCAT CCACCAGGGC CAGGAACTGC TGCAGTACGT CAACGAGGTG
    CAGGCGTCTG GTGTGGAGTT GCTGTGTGAC CGTGATGTGG ACATGGCCAC CCGTGTTCAG
    GACCTGCTAG AGTTTCTACA TGAGAAACAG CAAGAGCTGG ACGTGGCTGC AGAACAGCAT
    CGCAGACACC TTGAGCAGTG TGTGCAACTG CGCCACCTAC AAGCCGAAGT CAAACAGGTT
    CTGGGTTGGA TCCGTAATGG CGAGTCGATG CTGAATGCAG GTCTGATTAC GGCCAGCTCT
    CTTCAAGAAG CAGAGCAGTT GCAGAGAGAG CATGAGCAAT TCCAGCATGC TATCGAGAAA
    ACCCATCAGA GCGCCCTGCA GGTCCAGCAG AAAGCCGAAG CCCTGCTGCA GGCCAATCAC
    TACGACATGG ACATGATTCG AGACTGCGCG GAGAACGTGG CTTCCCACTG GCAGCAGCTC
    ATGCTGAAGA TGGAGGACCG GCTCAAGCTG GTCAACGCCT CCGTGGCCTT CTATAAGACC
    TCTGAACAGG TTTGCAGTGT TCTGGAGAGT CTGGAGCAGG AGTACAAGCG TGAGGAGGAC
    TGGTGCGGAG GAGCTGACAA ACTGGGACCC AACTGTGAAT CGGACCACGT CACACCCATG
    ATCAGCAAAC ACCTGGAGCA GAAAGAGGCT TTCCTCAAGG CCTGCACGTT GGCCAGGCGT
    AATGCAGACG TCTTCCTCAA ATACATGCAC AGGAACAGCG TCAGCATGCC CGGGATGTTG
    AGTCACGTCA AAGCTCCAGA GCAGCAGGTC AAAAATATCC TGAATGAGCT GCTGCAGAGG
    GAGAACCGCG TGCTGCACTT CTGGACTATG AGAAAGAGGA GGCTGGACCA GTGTCAGCAA
    TACGTGGTGT TTGAACGCAG CGCCAAACAG GCTCTGGAGT GGATCCACGA CACTGGCGAG
    TTTTATCTGT CCACACACAC GTCCACCGGC TCCAGCATAC ACCACACACA GGAGCTGCTG
    AAGGAGCACG AGGACTTCCA CATCACAGCC AAGCAAACCA AAGAACGGGT GAAGCTGCTG
    ATCCAGCTGG CAGATGGTTT CTGTGATAAG GGTCACTCTC ACGCCGCTGA GATTAAGAAA
    TGGGTGACAG CGGTGGACAA ACGCTATCGA GATTTCTCGC TCCGCATGGA CAAGTACCGC
    ACATCTCTGG AGAAAGCACT CGGCATCTCC TCAGACTCCA ATAAAGCTAG CAAAGACTTG
    CAGCTGGACA TCATCCCTGC CAGTGCTCCG GGGTCAGAGG TCAAACTCAG AGACGCTGCT
    CATGAGCTCA ATGAAGAGAA GCGCAAGTCT GCCCGCAGGA AGGACTTCAT CATGGCTGAG
    CTGATCCAGA CAGAAAAGGC CTATGTGCGA GACTTGAGAG AGTGCATGGA CACGTATCTG
    TGGGAGATGA CCAGCGGCGT GGAGGAGATC CCACCAGGCA TCGTCAACAA AGAGCACATC
    ATTTTTGGTA ACATGCAAGA TCTGTACGAG TTCCATCACA ATATATTCCT CAAAGAGCTG
    GAGAAATACG AGCAGCTTCC TGAAGATGTG GGCCACTGTT TTGTGACTTG GGCTGATAAG
    TTCCAGATGT ATGTGAACTA CTGCAAGAAC AAACCAGACT CCACTCAGCT TATTCTTGAA
    CATGCCGGAG GATACTTCGA CGAGATCCAG CAGAGGCATC GCCTGGCCAA CTCCATCTCC
    TCATACCTCA TCAAACCTGT TCAGAGAATA ACCAAGTACC AGCTGCTCCT CAAGGAGCTC
    CTGACGTGCT GCGAGGAAGG CAAAGGTGAA ATTAAAGATG GCCTGGAGGT GATGCTCAGT
    GTTCCCAAAA GAGCCAACGA CGCCATGCAC TTGAGCATGC TTGAAGGGTT TGATGAGAAC
    ATCGAGTCCC AGGGAGAGCT GATCCTGCAG GAGGCGTTTC AGGTGTGGGA TCCCAAAACT
    CTGATCCGCA AGGGAAGAGA ACGACACCTG TTCCTCTTCG AGATGTCACT GATCTTCAGC
    AAGGAGGTCA AAGACTCCAA TGGCAGGAGC AAGTACATCT ACAAGAGCAA GCTTTTCACC
    TCTGAACTGG GTGTCACTGA GCATGTGGAG GGAGATCCAT GCAAATTTGC TCTCTGGGTT
    GGACGAACCC CAACTTCTGA CAACAAGATT GTCCTCAAGC TAAGTCAGGG ATGCAAACAT
    GGATCATTGG CGTCCGGTAT TGAGAACAAA CAGGACTGGA TCAAGCACAT CCGGGAAGTG
    ATTCAGGAGC GCACCGTTCA CCTGAAGGGG GCGCTGAAAG AGCCCATTCA CATCCCCAAA
    GCCTCCACAG CCAAACACAA GGGGCGCAGG TACACCCCAA GGGATGGAGA GGATCTGGAC
    AGTCAGGGTG ACGGCAGCAG TCAACCAGAC ACCATCTCAC TGGCCTCCAG GACCTCACAG
    AACACACTGG ACAGCGACAA GTTGTCTGGT GGCTGTGAGC TGACGGTGGT CATCCATGAC
    TTTATGGCCG GTAACAGTAA TGAGCTGACC ATCCGGAGAG GACAGACGGT GGAGGTGCTC
    GAGAGACTTC ATGATAAGCC GGACTGGTGT TTGGTTCGCA CCACAGACCG TTCGCCGGCG
    CTGGAGGGCC TGGTGCCCTG TGCCATGCTC TGTATCGCCC ACTCCCGCAG CAGCATGGAG
    ATGGAGGGCA TCTTCAACCA CAAAGATGCA CTCTCTGTAT GCAGTAATGA TGCCATCATG
    CCAGGCTCCT CTGCCACCCT GCAGCCGGGT CATGTGATGG GTTCCCAGAG TTCTCCTGGA
    CCTAAAAGGC CTGGCAACAC ATTAAGGAAA TGGCTGACCA GTCCTGTGAG GAGACTGAGC
    AGCGGTAAAG CTGACGGACA CGTCAAGAAG CTGGCACACA AACACAAGAA GAGCCGTGAC
    GTCAGAAAGA GCGCAGACGC CGGTTCACAG AAAGACTCGG ACGACAGCGC TGCGACGCCA
    CAAGATGAGA CCCTCGAGGA GAGGGTGCGT AATGAGGGTC TGAGCAGCGG CACGCTCTCC
    AAATCCTCCT CTTCAGGGAT GCAGAGCTGT GGAGAGGAAG AGGGAGAGGA AGGAGCTGAC
    GCTGTGCCTC TTCCTCCACC AATGGCCATC CAGCAGCACA GCCTCCTCCA GCCGGATGCG
    CAGGATGACA AGACGTCTTC ACGTCTTTCC GTCAGACCCT CCAGTTCTGA GACCCCCAGC
    GCTGCAGAGC TCGTCAGTGC CATTGAGGAG CTGGTCAAAA GCAAGATGGC TCTGGAAGAC
    AGACCCAGCA CTCTGTCAGT GGAGCAGGGC GACAGCAGCA GCCCTTCATT CAACCCATCT
    GATAACTCTC TCCTGTCCTC CTCCTCTCCA ATCGATGAGA TAGAGGAACG CAAGACCGGT
    TTCTTCAAGA AGCGCCATTA CGTCCTGCTG GAGCTGATCG AGACGGAGAG AGACTATGTG
    CGAGATCTCA GTTTGGTCGT GGAGGGCTAC ATGGCCAGGA TGAGGGAGGA CGGGGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATCGTT TTTGGAAACA TTCAGCAGAT ATATGACTGG
    CACAAAGATT TCTTCCTGGG AGAGCTTGAG AAGTGCCTTG AGGACCCTGA CCGGCTGGGC
    CCTCTCTTCC TCAAACATGA GCGGAGACTG CACATGTACA TTGTTTACTG CCAGAACAAA
    CCTAAATCTG AACACATCGT CTCAGAGTAC ATCGACACAT ATTTTGAGGA TCTGAAGCAG
    CGTTTGGGTC ACAGGCTTCA GATCACTGAT CTGCTCATTA AACCTGTACA GAGAATCATG
    AAGTACCAGC TGCTGCTGAA GGATTTCCTC AAATTTTCCA AAAAAATCGG AACAGATTCG
    ATTGAATTAG AGAAAGCGGT TGAGGTCATG TGCATTGTAC CAAAAAGATG CAACGACATG
    ATGAATGTTG GTCGACTCCA GGGGTTTGAT GGAAAGATTG TTGCTCAGGG GCGTCTCCTA
    CTCCAGGACA CCTTCATGGT GGCCGAACCA GAGGGAGGCC TGCTGAACAG GATGAAGGAG
    AGGAGAGTCT TCCTCTTTGA GCAGATCGTC ATCTTCAGTG AACCTCTAGA TAAAAAGAGG
    GGCTTCTCCA TGCCTGGCTA TTTGTACAAA TACAGCATCA AGGTGAACTG TCTGGGACTT
    GAGGACAGTG TGGACGGAGA CCCCTGTAAA TTTGCACTGA CCTCCAGAAC GTCCAATAGC
    AGCAAAGATG CCTTCATTCT GCACTCGAGC CATCCTGGAG TACGACAAGT CTGGATGCTG
    CAGATCAGCC AAATCCTGGA GAGCCAACGC AATTTCCTCA ATGCACTTAC ATCTCCTATA
    GACTACCAGA GGAACCACGT GGAGGGTCCT GGTGTGTCAG GCAGCGGTGT ACAGGCTGGA
    GGCAGCGGGG GGCAGATGAT GGCCCCTGGA GGAGGAGTAG GGGTCCCGGC TGGGCCGGGT
    TCCCGTTCTC GTCCGTCCCG TATCCCCCAG CCCTCGCGCT TGCCTCAACC GCTCCGTCAT
    CACTCTCCTG CCCTGGGGCC TGGAGCCCAC GAGCATGACG GCCCCGACAA ACTATCAGGT
    ATGTCCCCAA GACCTCTCTC CAGGGGTCCC TCACCCTCAT GCACCACAGA GCCCGAGCCA
    AAGGTGAAGC TTCCTGCGAG CCCTCACCCC AAACAGACGG ACTCCCAACA GACAGAAAGC
    CCAGCCAAAG AGATCCCACG AGCCACTGTG GCCCCGCTGG CTCTTGTCAA ACCCAGACCT
    GGAACGGTTT CACCAATGGC CTCTCCGTTA GCCACACCGG CTTTCAAAGA CTCCATTCCA
    CCCTGTAGTC CAGGCCCGAA GACTGGCAGC AGCTCGTTTT GGAGCTCAGT GCCCGCGTCC
    CCCGCCAGCA GGCCTGCTTC CTTCACCTTC CCCGGGGACG CCTGCGACAC GCTGAGCCGA
    CCAAATCACA ACCAGAGTCA GCGGCACTCC ACCCACAGTA AAGACGCAGA CCGTATGAGC
    ACCTGCTCCT CTACCAGTGA GCAGTCTATC CAGTCCACTC AGAGCAATGG GAGTGAAAGC
    AGCAGCAGCA GTAATATCTC CACCATGTTG GTGACTCAGG ACTATGTGGC TCTGAAAGAG
    GATGAGATTA ATGTGGGTCA GGGGGAGGTG GTGCAGATCC TGGCCTCCAA TCAGCAGAAT
    ATGTTCTTGG TGTTTCGTGC CGCTACTGAG CAGTGTCCTG CCGCCGAGGG CTGGATCCCC
    GGATACGTGC TGGGACACAC ATCCGCCATC ATCCCTGACG CTCCAGACGG AACCATAAAC
    AGGAAATCGT CATCATCATG GCACACATCC CTCCGTATTC GAAAGAAGTC AGAGAAAAGA
    GAAAAGGACA ATAAAAAAGA GGCCAAGCTG GAAAACGGCT ACCGGAAATC CAGAGAGGGA
    CTGACCAACA AGGTCTCTGT GAAGCTTCTA AATCCCAATT ATATATATGA TGTTCTCCCG
    GAGTTCCTCG TCCCGCTGAG TGACGTGACC TGTGATAAAG GCGAGTGTGT GACTCTGAGG
    TGTAAAGTGT GTGGTCGGCC CAAAGCCACG GTCACGTGGA AGGGACCTGA GCAGAAAACA
    CTCACCAACA ACGGCCACTT CAGCATCGCC TACAGTGAGA CTGGTGAGGC CACGCTGCGG
    ATCGTAGGTG TGACCTCAGA GGATGATGGC AGTTACACCT GCATTGCCAC GAATGACATT
    GGCAGTGTGG CATCATCAGC CAGTCTCAGA GTGCTGGGCA CGACCAGCGA TGGGATCCGG
    GTCATGTGGA AAGACAACTT TGAGTCCTTC TACACTGAGG TAGCAGAGCT TGGCAGGGGA
    CGGTTCTCTG TAGTGAAACG CTGTGACCAG AGGGGATCAA AGCGGACCGT GGCGGTCAAG
    TTTGTCAATA AGAAACTGAT GAAGCGAGAC CAGGTGACCC ATGAGTTCAG TGTCCTACAG
    AGACTCCAGC ACCCTCATCT GGTCAGACTG CTGGACACCT TCGAGACCTC CAGCAGCTAC
    GCACTGGTCC TGGAGATGTC TGATCAGGGC CGTCTGCTGG ACTACATCGT GAGCTGGGGG
    AATCTCACTG AGGAGAAAGT GGCCTTTTAC CTCCGGGACA TTTTAGAAGC TTTGCAGTAC
    CTGCACAACT GCAGAATAGT CCATCTGGAT CTAAAGCCTG AGAATCTGGT GGTGGAGCAG
    AGCCCGTCTC AGCCGCTGGT CAAGCTGACG GACTTCGGTG ATGCTGTGCA GCTCAACTCC
    ACGCCGTACG TTCACCCTCT GCTGGGGAGC CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC
    GGGGACCCTG TCTCTCTGTC CTCCGACCTG TGGAGTCTGG GGGTCCTGAC GTACGTCATG
    CTGAGCGGAG CATCTCCGTT CCTTGATGAG AGCGTGGAGG AGACCTGCCT CAACATCTGC
    CGCTTGGACT TCAGCTTTCC TGATGACTAC TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT
    TTTATGTGCC TGCTGCTCCG GATGGAGCCA TCCAAACGTC CACCAGCCAC CTCATGTCTA
    CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC CGACGCTCAG CCGAATGCAT CGATACCTCC
    AGACTGATCT CCTTCATCGA CCGACGCAAA CACCAGAACG ACCTGCGGCC TCTCACTGGC
    ATCAGAGCCT TCCTGCAGAC TCGATTACAG CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292694.1
    CDS389..9310
    Translation

    Target ORF information:

    RefSeq Version XM_009294419.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294419.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGCGTGGCT CCAAATGGGA TTCCATCAAG CCTCTGCTGA AGATCCTCCA GGAGTCGTTC 
    CCCTGCTGCA TCCACATCGC CCTCATCATC AAACCAGACA ACTTCTGGCA GAAACAGAGG
    ACCAATTTCG GCAGCTCCAA GTTTGAGTTT GAGACCACAA TGGTTTCTTT GGAGGGCTTA
    TCGAAGGTGG TGGACCCATC CCAGTTGACG GCTGACTTTG AGGGCAGCCT GGACTACAAC
    CATGAAGAGT GGATTGAAAT CCGCGTGGCC TTCGAAGACT TTACAGGCAA TGCTCGGCAC
    CTGCTCGCCC GGCTAGAAGA GATGCATGAA ACGGTGACGC GTAAAGACTT ACCGCAGGAC
    TTAGATGGAG CCCGACGGAT GATAGAGGAG CACGCGGCTT TGAAGAAGAG GGTGATAAAG
    GCTCCGGTGG AGGAGGTGGA CAATGAGGGC CAAAGGCTCC TGCAAAGGAT CCAGAGCAGT
    GAGAGCTACG CCAACCGAAC TGTGCCTGTG CCGCCTGGTC AGAGGGAAGG GCAGGGGCAG
    CCCAACGCCG ACACCCAGGG CCTGGTGCCC CGTATTACAG CTCTGCTGGA GAAACTGCAC
    AGCACCAGGC AGAACCTGCA CCAGTCCTGG CACATCCGCA AGCTGCAGCT GGACCAGTGC
    TTCCAGCTCA GGCTGTTCGA GCAGGACGCT GAGAAGATGT TTGATTGGAT CATGCACAAT
    AAAGGGCTGT TTCTGGCTGG ATACACTGAG ATCGGCAACA ACCACCCTCA CGCCATGGAG
    CTGCAGACCC AGCACAATCA CTTCGCCATG AACTGCATGA ATGTTTACGT GAACATAAAC
    CGCATCATGT CTGTGGGGAA CCGGCTGCTG GAGGCCGGTC ATTACGCGTC GCAGCAGATC
    AAGCAGATCT CAGGTCAGCT GGAGCAGGAG TGGAAAGCTT TCGCCGCTGC GCTGGACGAG
    AGGAGTGCTC TGCTGGAGAT GTCTGCTACC TTCCACCAGA AATGCGACCA GTACATGAGT
    AATGTGGACT CGTGGTGTAA AGCGTGTGGA GAAGTGGATC TGCCCTCAGA GCTGCAGGAA
    CTGGAGGACG CTATACACCA TCACCAGGGC CTGTATGAAC ACATCACCGC TGCCTACTCG
    GAGGTGAGCC AAGACGGAAA AGCCTTATTG GACAAACTTC AGCGCCCCTT GACGCCCGGC
    AGTGCTGATT CGCTGACAGC TTCCGCCAAT TACACCAAGG CGGTGAACCA TGTTTTGGAC
    ATCATCCATG AGGTTCTGCA TCACCAAAGG CAGCTGGAGA ACATCTGGCA ACATCGCAAG
    GTCCGGCTGC ACCAGCGTCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTTCTG
    GACTGGATTG AGAACCATGG CGAGGCATTT CTCAGCAAGC ACACAGGGGT GGGAAAATCT
    CTGCATCGCG CTCGAGCTCT GCAGAAACGC CATGAAGACT TTGAGGAAGT GGCTCAGAAC
    ACGTACACAA ATGCGGATAA GCTTCTGGAA GCGGCAGAGC AGCTGGCCCA GACAGGCGAG
    TGTGACCCAG AGGAGATCTA TCAGGCCGCT CATCAGCTGG AGGACCGAAT CCAGGACTTT
    GTTCGGCGCG TGGAGCAGCG CAAGGTCCTG CTGGACATGT CAGTGGCTTT CCACACTCAC
    GTCAAAGAGT TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGTTGCTGGA CGATGTGTAC
    GCCGAGTCTG TGGAAGCCGT GCAGGATCTG ATCAAGCGTT TCGGGCAGCA GCAACAAACC
    ACACTGCAGT TCACTGTAAA CGTCATCAAA GAAGGAGAAG ATCTCATCCA GCAGCTCAGA
    GACCTGGCCA TCTCCAGCAA CAAGACGCCT CACAACAGCT CCATCAACCA CATCGAGAGC
    GTCCTGCAGC AGCTGGATGA GGCTCAGGCT CAGATGGAGG AGCTTTTCCA GGAGAGGAAG
    ATCAAGCTGG AGCTTTTCCT GCAGCTCAGG ATCTTTGAGC GGGACGCCAT AGATATTATC
    TCAGATCTGG ACTCGTGGAA TGAGGAGCTG TCGCAGCAGA TGAATGAGTT TGATACAGAA
    GACCTGACGC TGGCTGAACA GAGACTGCAG CACCATGCTG ATAAAGCCCT GACCATGAAC
    AACCTGGCCT TCCACGTCAT CCACCAGGGC CAGGAACTGC TGCAGTACGT CAACGAGGTG
    CAGGCGTCTG GTGTGGAGTT GCTGTGTGAC CGTGATGTGG ACATGGCCAC CCGTGTTCAG
    GACCTGCTAG AGTTTCTACA TGAGAAACAG CAAGAGCTGG ACGTGGCTGC AGAACAGCAT
    CGCAGACACC TTGAGCAGTG TGTGCAACTG CGCCACCTAC AAGCCGAAGT CAAACAGGTT
    CTGGGTTGGA TCCGTAATGG CGAGTCGATG CTGAATGCAG GTCTGATTAC GGCCAGCTCT
    CTTCAAGAAG CAGAGCAGTT GCAGAGAGAG CATGAGCAAT TCCAGCATGC TATCGAGAAA
    ACCCATCAGA GCGCCCTGCA GGTCCAGCAG AAAGCCGAAG CCCTGCTGCA GGCCAATCAC
    TACGACATGG ACATGATTCG AGACTGCGCG GAGAACGTGG CTTCCCACTG GCAGCAGCTC
    ATGCTGAAGA TGGAGGACCG GCTCAAGCTG GTCAACGCCT CCGTGGCCTT CTATAAGACC
    TCTGAACAGG TTTGCAGTGT TCTGGAGAGT CTGGAGCAGG AGTACAAGCG TGAGGAGGAC
    TGGTGCGGAG GAGCTGACAA ACTGGGACCC AACTGTGAAT CGGACCACGT CACACCCATG
    ATCAGCAAAC ACCTGGAGCA GAAAGAGGCT TTCCTCAAGG CCTGCACGTT GGCCAGGCGT
    AATGCAGACG TCTTCCTCAA ATACATGCAC AGGAACAGCG TCAGCATGCC CGGGATGTTG
    AGTCACGTCA AAGCTCCAGA GCAGCAGGTC AAAAATATCC TGAATGAGCT GCTGCAGAGG
    GAGAACCGCG TGCTGCACTT CTGGACTATG AGAAAGAGGA GGCTGGACCA GTGTCAGCAA
    TACGTGGTGT TTGAACGCAG CGCCAAACAG GCTCTGGAGT GGATCCACGA CACTGGCGAG
    TTTTATCTGT CCACACACAC GTCCACCGGC TCCAGCATAC ACCACACACA GGAGCTGCTG
    AAGGAGCACG AGGACTTCCA CATCACAGCC AAGCAAACCA AAGAACGGGT GAAGCTGCTG
    ATCCAGCTGG CAGATGGTTT CTGTGATAAG GGTCACTCTC ACGCCGCTGA GATTAAGAAA
    TGGGTGACAG CGGTGGACAA ACGCTATCGA GATTTCTCGC TCCGCATGGA CAAGTACCGC
    ACATCTCTGG AGAAAGCACT CGGCATCTCC TCAGACTCCA ATAAAGCTAG CAAAGACTTG
    CAGCTGGACA TCATCCCTGC CAGTGCTCCG GGGTCAGAGG TCAAACTCAG AGACGCTGCT
    CATGAGCTCA ATGAAGAGAA GCGCAAGTCT GCCCGCAGGA AGGACTTCAT CATGGCTGAG
    CTGATCCAGA CAGAAAAGGC CTATGTGCGA GACTTGAGAG AGTGCATGGA CACGTATCTG
    TGGGAGATGA CCAGCGGCGT GGAGGAGATC CCACCAGGCA TCGTCAACAA AGAGCACATC
    ATTTTTGGTA ACATGCAAGA TCTGTACGAG TTCCATCACA ATATATTCCT CAAAGAGCTG
    GAGAAATACG AGCAGCTTCC TGAAGATGTG GGCCACTGTT TTGTGACTTG GGCTGATAAG
    TTCCAGATGT ATGTGAACTA CTGCAAGAAC AAACCAGACT CCACTCAGCT TATTCTTGAA
    CATGCCGGAG GATACTTCGA CGAGATCCAG CAGAGGCATC GCCTGGCCAA CTCCATCTCC
    TCATACCTCA TCAAACCTGT TCAGAGAATA ACCAAGTACC AGCTGCTCCT CAAGGAGCTC
    CTGACGTGCT GCGAGGAAGG CAAAGGTGAA ATTAAAGATG GCCTGGAGGT GATGCTCAGT
    GTTCCCAAAA GAGCCAACGA CGCCATGCAC TTGAGCATGC TTGAAGGGTT TGATGAGAAC
    ATCGAGTCCC AGGGAGAGCT GATCCTGCAG GAGGCGTTTC AGGTGTGGGA TCCCAAAACT
    CTGATCCGCA AGGGAAGAGA ACGACACCTG TTCCTCTTCG AGATGTCACT GATCTTCAGC
    AAGGAGGTCA AAGACTCCAA TGGCAGGAGC AAGTACATCT ACAAGAGCAA GCTTTTCACC
    TCTGAACTGG GTGTCACTGA GCATGTGGAG GGAGATCCAT GCAAATTTGC TCTCTGGGTT
    GGACGAACCC CAACTTCTGA CAACAAGATT GTCCTCAAGC TAAGTCAGGG ATGCAAACAT
    GGATCATTGG CGTCCGGTAT TGAGAACAAA CAGGACTGGA TCAAGCACAT CCGGGAAGTG
    ATTCAGGAGC GCACCGTTCA CCTGAAGGGG GCGCTGAAAG AGCCCATTCA CATCCCCAAA
    GCCTCCACAG CCAAACACAA GGGGCGCAGG TACACCCCAA GGGATGGAGA GGATCTGGAC
    AGTCAGGGTG ACGGCAGCAG TCAACCAGAC ACCATCTCAC TGGCCTCCAG GACCTCACAG
    AACACACTGG ACAGCGACAA GTTGTCTGGT GGCTGTGAGC TGACGGTGGT CATCCATGAC
    TTTATGGCCG GTAACAGTAA TGAGCTGACC ATCCGGAGAG GACAGACGGT GGAGGTGCTC
    GAGAGACTTC ATGATAAGCC GGACTGGTGT TTGGTTCGCA CCACAGACCG TTCGCCGGCG
    CTGGAGGGCC TGGTGCCCTG TGCCATGCTC TGTATCGCCC ACTCCCGCAG CAGCATGGAG
    ATGGAGGGCA TCTTCAACCA CAAAGATGCA CTCTCTGTAT GCAGTAATGA TGCCATCATG
    CCAGGCTCCT CTGCCACCCT GCAGCCGGGT CATGTGATGG GTTCCCAGAG TTCTCCTGGA
    CCTAAAAGGC CTGGCAACAC ATTAAGGAAA TGGCTGACCA GTCCTGTGAG GAGACTGAGC
    AGCGGTAAAG CTGACGGACA CGTCAAGAAG CTGGCACACA AACACAAGAA GAGCCGTGAC
    GTCAGAAAGA GCGCAGACGC CGGTTCACAG AAAGACTCGG ACGACAGCGC TGCGACGCCA
    CAAGATGAGA CCCTCGAGGA GAGGGTGCGT AATGAGGGTC TGAGCAGCGG CACGCTCTCC
    AAATCCTCCT CTTCAGGGAT GCAGAGCTGT GGAGAGGAAG AGGGAGAGGA AGGAGCTGAC
    GCTGTGCCTC TTCCTCCACC AATGGCCATC CAGCAGCACA GCCTCCTCCA GCCGGATGCG
    CAGGATGACA AGACGTCTTC ACGTCTTTCC GTCAGACCCT CCAGTTCTGA GACCCCCAGC
    GCTGCAGAGC TCGTCAGTGC CATTGAGGAG CTGGTCAAAA GCAAGATGGC TCTGGAAGAC
    AGACCCAGCA CTCTGTCAGT GGAGCAGGGC GACAGCAGCA GCCCTTCATT CAACCCATCT
    GATAACTCTC TCCTGTCCTC CTCCTCTCCA ATCGATGAGA TAGAGGAACG CAAGACCGGT
    TTCTTCAAGA AGCGCCATTA CGTCCTGCTG GAGCTGATCG AGACGGAGAG AGACTATGTG
    CGAGATCTCA GTTTGGTCGT GGAGGGCTAC ATGGCCAGGA TGAGGGAGGA CGGGGTTCCA
    GATGATATGA AAGGAAAAGA CAAGATCGTT TTTGGAAACA TTCAGCAGAT ATATGACTGG
    CACAAAGATT TCTTCCTGGG AGAGCTTGAG AAGTGCCTTG AGGACCCTGA CCGGCTGGGC
    CCTCTCTTCC TCAAACATGA GCGGAGACTG CACATGTACA TTGTTTACTG CCAGAACAAA
    CCTAAATCTG AACACATCGT CTCAGAGTAC ATCGACACAT ATTTTGAGGA TCTGAAGCAG
    CGTTTGGGTC ACAGGCTTCA GATCACTGAT CTGCTCATTA AACCTGTACA GAGAATCATG
    AAGTACCAGC TGCTGCTGAA GGATTTCCTC AAATTTTCCA AAAAAATCGG AACAGATTCG
    ATTGAATTAG AGAAAGCGGT TGAGGTCATG TGCATTGTAC CAAAAAGATG CAACGACATG
    ATGAATGTTG GTCGACTCCA GGGGTTTGAT GGAAAGATTG TTGCTCAGGG GCGTCTCCTA
    CTCCAGGACA CCTTCATGGT GGCCGAACCA GAGGGAGGCC TGCTGAACAG GATGAAGGAG
    AGGAGAGTCT TCCTCTTTGA GCAGATCGTC ATCTTCAGTG AACCTCTAGA TAAAAAGAGG
    GGCTTCTCCA TGCCTGGCTA TTTGTACAAA TACAGCATCA AGGTGAACTG TCTGGGACTT
    GAGGACAGTG TGGACGGAGA CCCCTGTAAA TTTGCACTGA CCTCCAGAAC GTCCAATAGC
    AGCAAAGATG CCTTCATTCT GCACTCGAGC CATCCTGGAG TACGACAAGT CTGGATGCTG
    CAGATCAGCC AAATCCTGGA GAGCCAACGC AATTTCCTCA ATGCACTTAC ATCTCCTATA
    GACTACCAGA GGAACCACGT GGAGGGTCCT GGTGTGTCAG GCAGCGGTGT ACAGGCTGGA
    GGCAGCGGGG GGCAGATGAT GGCCCCTGGA GGAGGAGTAG GGGTCCCGGC TGGGCCGGGT
    TCCCGTTCTC GTCCGTCCCG TATCCCCCAG CCCTCGCGCT TGCCTCAACC GCTCCGTCAT
    CACTCTCCTG CCCTGGGGCC TGGAGCCCAC GAGCATGACG GCCCCGACAA ACTATCAGGT
    ATGTCCCCAA GACCTCTCTC CAGGGGTCCC TCACCCTCAT GCACCACAGA GCCCGAGCCA
    AAGGTGAAGC TTCCTGCGAG CCCTCACCCC AAACAGACGG ACTCCCAACA GACAGAAAGC
    CCAGCCAAAG AGATCCCACG AGCCACTGTG GCCCCGCTGG CTCTTGTCAA ACCCAGACCT
    GGAACGGTTT CACCAATGGC CTCTCCGTTA GCCACACCGG CTTTCAAAGA CTCCATTCCA
    CCCTGTAGTC CAGGCCCGAA GACTGGCAGC AGCTCGTTTT GGAGCTCAGT GCCCGCGTCC
    CCCGCCAGCA GGCCTGCTTC CTTCACCTTC CCCGGGGACG CCTGCGACAC GCTGAGCCGA
    CCAAATCACA ACCAGAGTCA GCGGCACTCC ACCCACAGTA AAGACGCAGA CCGTATGAGC
    ACCTGCTCCT CTACCAGTGA GCAGTCTATC CAGTCCACTC AGAGCAATGG GAGTGAAAGC
    AGCAGCAGCA GTAATATCTC CACCATGTTG GTGACTCAGG ACTATGTGGC TCTGAAAGAG
    GATGAGATTA ATGTGGGTCA GGGGGAGGTG GTGCAGATCC TGGCCTCCAA TCAGCAGAAT
    ATGTTCTTGG TGTTTCGTGC CGCTACTGAG CAGTGTCCTG CCGCCGAGGG CTGGATCCCC
    GGATACGTGC TGGGACACAC ATCCGCCATC ATCCCTGACG CTCCAGACGG AACCATAAAC
    AGGAAATCGT CATCATCATG GCACACATCC CTCCGTATTC GAAAGAAGTC AGAGAAAAGA
    GAAAAGGACA ATAAAAAAGA GGCCAAGCTG GAAAACGGCT ACCGGAAATC CAGAGAGGGA
    CTGACCAACA AGGTCTCTGT GAAGCTTCTA AATCCCAATT ATATATATGA TGTTCTCCCG
    GAGTTCCTCG TCCCGCTGAG TGACGTGACC TGTGATAAAG GCGAGTGTGT GACTCTGAGG
    TGTAAAGTGT GTGGTCGGCC CAAAGCCACG GTCACGTGGA AGGGACCTGA GCAGAAAACA
    CTCACCAACA ACGGCCACTT CAGCATCGCC TACAGTGAGA CTGGTGAGGC CACGCTGCGG
    ATCGTAGGTG TGACCTCAGA GGATGATGGC AGTTACACCT GCATTGCCAC GAATGACATT
    GGCAGTGTGG CATCATCAGC CAGTCTCAGA GTGCTGGGCA CGACCAGCGA TGGGATCCGG
    GTCATGTGGA AAGACAACTT TGAGTCCTTC TACACTGAGG TAGCAGAGCT TGGCAGGGGA
    CGGTTCTCTG TAGTGAAACG CTGTGACCAG AGGGGATCAA AGCGGACCGT GGCGGTCAAG
    TTTGTCAATA AGAAACTGAT GAAGCGAGAC CAGGTGACCC ATGAGTTCAG TGTCCTACAG
    AGACTCCAGC ACCCTCATCT GGTCAGACTG CTGGACACCT TCGAGACCTC CAGCAGCTAC
    GCACTGGTCC TGGAGATGTC TGATCAGGGC CGTCTGCTGG ACTACATCGT GAGCTGGGGG
    AATCTCACTG AGGAGAAAGT GGCCTTTTAC CTCCGGGACA TTTTAGAAGC TTTGCAGTAC
    CTGCACAACT GCAGAATAGT CCATCTGGAT CTAAAGCCTG AGAATCTGGT GGTGGAGCAG
    AGCCCGTCTC AGCCGCTGGT CAAGCTGACG GACTTCGGTG ATGCTGTGCA GCTCAACTCC
    ACGCCGTACG TTCACCCTCT GCTGGGGAGC CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC
    GGGGACCCTG TCTCTCTGTC CTCCGACCTG TGGAGTCTGG GGGTCCTGAC GTACGTCATG
    CTGAGCGGAG CATCTCCGTT CCTTGATGAG AGCGTGGAGG AGACCTGCCT CAACATCTGC
    CGCTTGGACT TCAGCTTTCC TGATGACTAC TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT
    TTTATGTGCC TGCTGCTCCG GATGGAGCCA TCCAAACGTC CACCAGCCAC CTCATGTCTA
    CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC CGACGCTCAG CCGAATGCAT CGATACCTCC
    AGACTGATCT CCTTCATCGA CCGACGCAAA CACCAGAACG ACCTGCGGCC TCTCACTGGC
    ATCAGAGCCT TCCTGCAGAC TCGATTACAG CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42642
    Clone ID Related Accession (Same CDS sequence) XM_009294421.2 , XM_009294421.3
    Accession Version XM_009294421.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product triple functional domain protein isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGACGTC TTCACGTCTT
    TCCGTCAGAC CCTCCAGTTC TGAGACCCCC AGCGCTGCAG AGCTCGTCAG TGCCATTGAG
    GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA GACAGACCCA GCACTCTGTC AGTGGAGCAG
    GGCGACAGCA GCAGCCCTTC ATTCAACCCA TCTGATAACT CTCTCCTGTC CTCCTCCTCT
    CCAATCGATG AGATAGAGGA ACGCAAGACC GGTTTCTTCA AGAAGCGCCA TTACGTCCTG
    CTGGAGCTGA TCGAGACGGA GAGAGACTAT GTGCGAGATC TCAGTTTGGT CGTGGAGGGC
    TACATGGCCA GGATGAGGGA GGACGGGGTT CCAGATGATA TGAAAGGAAA AGACAAGATC
    GTTTTTGGAA ACATTCAGCA GATATATGAC TGGCACAAAG ATTTCTTCCT GGGAGAGCTT
    GAGAAGTGCC TTGAGGACCC TGACCGGCTG GGCCCTCTCT TCCTCAAACA TGAGCGGAGA
    CTGCACATGT ACATTGTTTA CTGCCAGAAC AAACCTAAAT CTGAACACAT CGTCTCAGAG
    TACATCGACA CATATTTTGA GGATCTGAAG CAGCGTTTGG GTCACAGGCT TCAGATCACT
    GATCTGCTCA TTAAACCTGT ACAGAGAATC ATGAAGTACC AGCTGCTGCT GAAGGATTTC
    CTCAAATTTT CCAAAAAAAT CGGAACAGAT TCGATTGAAT TAGAGAAAGC GGTTGAGGTC
    ATGTGCATTG TACCAAAAAG ATGCAACGAC ATGATGAATG TTGGTCGACT CCAGGGGTTT
    GATGGAAAGA TTGTTGCTCA GGGGCGTCTC CTACTCCAGG ACACCTTCAT GGTGGCCGAA
    CCAGAGGGAG GCCTGCTGAA CAGGATGAAG GAGAGGAGAG TCTTCCTCTT TGAGCAGATC
    GTCATCTTCA GTGAACCTCT AGATAAAAAG AGGGGCTTCT CCATGCCTGG CTATTTGTAC
    AAATACAGCA TCAAGGTGAA CTGTCTGGGA CTTGAGGACA GTGTGGACGG AGACCCCTGT
    AAATTTGCAC TGACCTCCAG AACGTCCAAT AGCAGCAAAG ATGCCTTCAT TCTGCACTCG
    AGCCATCCTG GAGTACGACA AGTCTGGATG CTGCAGATCA GCCAAATCCT GGAGAGCCAA
    CGCAATTTCC TCAATGCACT TACATCTCCT ATAGACTACC AGAGGAACCA CGTGGAGGGT
    CCTGGTGTGT CAGGCAGCGG TGTACAGGCT GGAGGCAGCG GGGGGCAGAT GATGGCCCCT
    GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG GGTTCCCGTT CTCGTCCGTC CCGTATCCCC
    CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT CATCACTCTC CTGCCCTGGG GCCTGGAGCC
    CACGAGCATG ACGGCCCCGA CAAACTATCA GGTATGTCCC CAAGACCTCT CTCCAGGGGT
    CCCTCACCCT CATGCACCAC AGAGCCCGAG CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC
    CCCAAACAGA CGGACTCCCA ACAGACAGAA AGCCCAGCCA AAGAGATCCC ACGAGCCACT
    GTGGCCCCGC TGGCTCTTGT CAAACCCAGA CCTGGAACGG TTTCACCAAT GGCCTCTCCG
    TTAGCCACAC CGGCTTTCAA AGACTCCATT CCACCCTGTA GTCCAGGCCC GAAGACTGGC
    AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG TCCCCCGCCA GCAGGCCTGC TTCCTTCACC
    TTCCCCGGGG ACGCCTGCGA CACGCTGAGC CGACCAAATC ACAACCAGAG TCAGCGGCAC
    TCCACCCACA GTAAAGACGC AGACCGTATG AGCACCTGCT CCTCTACCAG TGAGCAGTCT
    ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA AGCAGCAGCA GCAGTAATAT CTCCACCATG
    TTGGTGACTC AGGACTATGT GGCTCTGAAA GAGGATGAGA TTAATGTGGG TCAGGGGGAG
    GTGGTGCAGA TCCTGGCCTC CAATCAGCAG AATATGTTCT TGGTGTTTCG TGCCGCTACT
    GAGCAGTGTC CTGCCGCCGA GGGCTGGATC CCCGGATACG TGCTGGGACA CACATCCGCC
    ATCATCCCTG ACGCTCCAGA CGGAACCATA AAGAAATCGT CATCATCATG GCACACATCC
    CTCCGTATTC GAAAGAAGTC AGAGAAAAGA GAAAAGGACA ATAAAAAAGA GGCCAAGCTG
    GAAAACGGCT ACCGGAAATC CAGAGAGGGA CTGACCAACA AGGTCTCTGT GAAGCTTCTA
    AATCCCAATT ATATATATGA TGTTCTCCCG GAGTTCCTCG TCCCGCTGAG TGACGTGACC
    TGTGATAAAG GCGAGTGTGT GACTCTGAGG TGTAAAGTGT GTGGTCGGCC CAAAGCCACG
    GTCACGTGGA AGGGACCTGA GCAGAAAACA CTCACCAACA ACGGCCACTT CAGCATCGCC
    TACAGTGAGA CTGGTGAGGC CACGCTGCGG ATCGTAGGTG TGACCTCAGA GGATGATGGC
    AGTTACACCT GCATTGCCAC GAATGACATT GGCAGTGTGG CATCATCAGC CAGTCTCAGA
    GTGCTGGGCA CGACCAGCGA TGGGATCCGG GTCATGTGGA AAGACAACTT TGAGTCCTTC
    TACACTGAGG TAGCAGAGCT TGGCAGGGGA CGGTTCTCTG TAGTGAAACG CTGTGACCAG
    AGGGGATCAA AGCGGACCGT GGCGGTCAAG TTTGTCAATA AGAAACTGAT GAAGCGAGAC
    CAGGTGACCC ATGAGTTCAG TGTCCTACAG AGACTCCAGC ACCCTCATCT GGTCAGACTG
    CTGGACACCT TCGAGACCTC CAGCAGCTAC GCACTGGTCC TGGAGATGTC TGATCAGGGC
    CGTCTGCTGG ACTACATCGT GAGCTGGGGG AATCTCACTG AGGAGAAAGT GGCCTTTTAC
    CTCCGGGACA TTTTAGAAGC TTTGCAGTAC CTGCACAACT GCAGAATAGT CCATCTGGAT
    CTAAAGCCTG AGAATCTGGT GGTGGAGCAG AGCCCGTCTC AGCCGCTGGT CAAGCTGACG
    GACTTCGGTG ATGCTGTGCA GCTCAACTCC ACGCCGTACG TTCACCCTCT GCTGGGGAGC
    CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC GGGGACCCTG TCTCTCTGTC CTCCGACCTG
    TGGAGTCTGG GGGTCCTGAC GTACGTCATG CTGAGCGGAG CATCTCCGTT CCTTGATGAG
    AGCGTGGAGG AGACCTGCCT CAACATCTGC CGCTTGGACT TCAGCTTTCC TGATGACTAC
    TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT TTTATGTGCC TGCTGCTCCG GATGGAGCCA
    TCCAAACGTC CACCAGCCAC CTCATGTCTA CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC
    CGACGCTCAG CCGAATGCAT CGATACCTCC AGACTGATCT CCTTCATCGA CCGACGCAAA
    CACCAGAACG ACCTGCGGCC TCTCACTGGC ATCAGAGCCT TCCTGCAGAC TCGATTACAG
    CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292696.1
    CDS191..9442
    Translation

    Target ORF information:

    RefSeq Version XM_009294421.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294421.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGACGTC TTCACGTCTT
    TCCGTCAGAC CCTCCAGTTC TGAGACCCCC AGCGCTGCAG AGCTCGTCAG TGCCATTGAG
    GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA GACAGACCCA GCACTCTGTC AGTGGAGCAG
    GGCGACAGCA GCAGCCCTTC ATTCAACCCA TCTGATAACT CTCTCCTGTC CTCCTCCTCT
    CCAATCGATG AGATAGAGGA ACGCAAGACC GGTTTCTTCA AGAAGCGCCA TTACGTCCTG
    CTGGAGCTGA TCGAGACGGA GAGAGACTAT GTGCGAGATC TCAGTTTGGT CGTGGAGGGC
    TACATGGCCA GGATGAGGGA GGACGGGGTT CCAGATGATA TGAAAGGAAA AGACAAGATC
    GTTTTTGGAA ACATTCAGCA GATATATGAC TGGCACAAAG ATTTCTTCCT GGGAGAGCTT
    GAGAAGTGCC TTGAGGACCC TGACCGGCTG GGCCCTCTCT TCCTCAAACA TGAGCGGAGA
    CTGCACATGT ACATTGTTTA CTGCCAGAAC AAACCTAAAT CTGAACACAT CGTCTCAGAG
    TACATCGACA CATATTTTGA GGATCTGAAG CAGCGTTTGG GTCACAGGCT TCAGATCACT
    GATCTGCTCA TTAAACCTGT ACAGAGAATC ATGAAGTACC AGCTGCTGCT GAAGGATTTC
    CTCAAATTTT CCAAAAAAAT CGGAACAGAT TCGATTGAAT TAGAGAAAGC GGTTGAGGTC
    ATGTGCATTG TACCAAAAAG ATGCAACGAC ATGATGAATG TTGGTCGACT CCAGGGGTTT
    GATGGAAAGA TTGTTGCTCA GGGGCGTCTC CTACTCCAGG ACACCTTCAT GGTGGCCGAA
    CCAGAGGGAG GCCTGCTGAA CAGGATGAAG GAGAGGAGAG TCTTCCTCTT TGAGCAGATC
    GTCATCTTCA GTGAACCTCT AGATAAAAAG AGGGGCTTCT CCATGCCTGG CTATTTGTAC
    AAATACAGCA TCAAGGTGAA CTGTCTGGGA CTTGAGGACA GTGTGGACGG AGACCCCTGT
    AAATTTGCAC TGACCTCCAG AACGTCCAAT AGCAGCAAAG ATGCCTTCAT TCTGCACTCG
    AGCCATCCTG GAGTACGACA AGTCTGGATG CTGCAGATCA GCCAAATCCT GGAGAGCCAA
    CGCAATTTCC TCAATGCACT TACATCTCCT ATAGACTACC AGAGGAACCA CGTGGAGGGT
    CCTGGTGTGT CAGGCAGCGG TGTACAGGCT GGAGGCAGCG GGGGGCAGAT GATGGCCCCT
    GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG GGTTCCCGTT CTCGTCCGTC CCGTATCCCC
    CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT CATCACTCTC CTGCCCTGGG GCCTGGAGCC
    CACGAGCATG ACGGCCCCGA CAAACTATCA GGTATGTCCC CAAGACCTCT CTCCAGGGGT
    CCCTCACCCT CATGCACCAC AGAGCCCGAG CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC
    CCCAAACAGA CGGACTCCCA ACAGACAGAA AGCCCAGCCA AAGAGATCCC ACGAGCCACT
    GTGGCCCCGC TGGCTCTTGT CAAACCCAGA CCTGGAACGG TTTCACCAAT GGCCTCTCCG
    TTAGCCACAC CGGCTTTCAA AGACTCCATT CCACCCTGTA GTCCAGGCCC GAAGACTGGC
    AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG TCCCCCGCCA GCAGGCCTGC TTCCTTCACC
    TTCCCCGGGG ACGCCTGCGA CACGCTGAGC CGACCAAATC ACAACCAGAG TCAGCGGCAC
    TCCACCCACA GTAAAGACGC AGACCGTATG AGCACCTGCT CCTCTACCAG TGAGCAGTCT
    ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA AGCAGCAGCA GCAGTAATAT CTCCACCATG
    TTGGTGACTC AGGACTATGT GGCTCTGAAA GAGGATGAGA TTAATGTGGG TCAGGGGGAG
    GTGGTGCAGA TCCTGGCCTC CAATCAGCAG AATATGTTCT TGGTGTTTCG TGCCGCTACT
    GAGCAGTGTC CTGCCGCCGA GGGCTGGATC CCCGGATACG TGCTGGGACA CACATCCGCC
    ATCATCCCTG ACGCTCCAGA CGGAACCATA AAGAAATCGT CATCATCATG GCACACATCC
    CTCCGTATTC GAAAGAAGTC AGAGAAAAGA GAAAAGGACA ATAAAAAAGA GGCCAAGCTG
    GAAAACGGCT ACCGGAAATC CAGAGAGGGA CTGACCAACA AGGTCTCTGT GAAGCTTCTA
    AATCCCAATT ATATATATGA TGTTCTCCCG GAGTTCCTCG TCCCGCTGAG TGACGTGACC
    TGTGATAAAG GCGAGTGTGT GACTCTGAGG TGTAAAGTGT GTGGTCGGCC CAAAGCCACG
    GTCACGTGGA AGGGACCTGA GCAGAAAACA CTCACCAACA ACGGCCACTT CAGCATCGCC
    TACAGTGAGA CTGGTGAGGC CACGCTGCGG ATCGTAGGTG TGACCTCAGA GGATGATGGC
    AGTTACACCT GCATTGCCAC GAATGACATT GGCAGTGTGG CATCATCAGC CAGTCTCAGA
    GTGCTGGGCA CGACCAGCGA TGGGATCCGG GTCATGTGGA AAGACAACTT TGAGTCCTTC
    TACACTGAGG TAGCAGAGCT TGGCAGGGGA CGGTTCTCTG TAGTGAAACG CTGTGACCAG
    AGGGGATCAA AGCGGACCGT GGCGGTCAAG TTTGTCAATA AGAAACTGAT GAAGCGAGAC
    CAGGTGACCC ATGAGTTCAG TGTCCTACAG AGACTCCAGC ACCCTCATCT GGTCAGACTG
    CTGGACACCT TCGAGACCTC CAGCAGCTAC GCACTGGTCC TGGAGATGTC TGATCAGGGC
    CGTCTGCTGG ACTACATCGT GAGCTGGGGG AATCTCACTG AGGAGAAAGT GGCCTTTTAC
    CTCCGGGACA TTTTAGAAGC TTTGCAGTAC CTGCACAACT GCAGAATAGT CCATCTGGAT
    CTAAAGCCTG AGAATCTGGT GGTGGAGCAG AGCCCGTCTC AGCCGCTGGT CAAGCTGACG
    GACTTCGGTG ATGCTGTGCA GCTCAACTCC ACGCCGTACG TTCACCCTCT GCTGGGGAGC
    CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC GGGGACCCTG TCTCTCTGTC CTCCGACCTG
    TGGAGTCTGG GGGTCCTGAC GTACGTCATG CTGAGCGGAG CATCTCCGTT CCTTGATGAG
    AGCGTGGAGG AGACCTGCCT CAACATCTGC CGCTTGGACT TCAGCTTTCC TGATGACTAC
    TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT TTTATGTGCC TGCTGCTCCG GATGGAGCCA
    TCCAAACGTC CACCAGCCAC CTCATGTCTA CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC
    CGACGCTCAG CCGAATGCAT CGATACCTCC AGACTGATCT CCTTCATCGA CCGACGCAAA
    CACCAGAACG ACCTGCGGCC TCTCACTGGC ATCAGAGCCT TCCTGCAGAC TCGATTACAG
    CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42642
    Clone ID Related Accession (Same CDS sequence) XM_009294421.2 , XM_009294421.3
    Accession Version XM_009294421.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product triple functional domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGACGTC TTCACGTCTT
    TCCGTCAGAC CCTCCAGTTC TGAGACCCCC AGCGCTGCAG AGCTCGTCAG TGCCATTGAG
    GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA GACAGACCCA GCACTCTGTC AGTGGAGCAG
    GGCGACAGCA GCAGCCCTTC ATTCAACCCA TCTGATAACT CTCTCCTGTC CTCCTCCTCT
    CCAATCGATG AGATAGAGGA ACGCAAGACC GGTTTCTTCA AGAAGCGCCA TTACGTCCTG
    CTGGAGCTGA TCGAGACGGA GAGAGACTAT GTGCGAGATC TCAGTTTGGT CGTGGAGGGC
    TACATGGCCA GGATGAGGGA GGACGGGGTT CCAGATGATA TGAAAGGAAA AGACAAGATC
    GTTTTTGGAA ACATTCAGCA GATATATGAC TGGCACAAAG ATTTCTTCCT GGGAGAGCTT
    GAGAAGTGCC TTGAGGACCC TGACCGGCTG GGCCCTCTCT TCCTCAAACA TGAGCGGAGA
    CTGCACATGT ACATTGTTTA CTGCCAGAAC AAACCTAAAT CTGAACACAT CGTCTCAGAG
    TACATCGACA CATATTTTGA GGATCTGAAG CAGCGTTTGG GTCACAGGCT TCAGATCACT
    GATCTGCTCA TTAAACCTGT ACAGAGAATC ATGAAGTACC AGCTGCTGCT GAAGGATTTC
    CTCAAATTTT CCAAAAAAAT CGGAACAGAT TCGATTGAAT TAGAGAAAGC GGTTGAGGTC
    ATGTGCATTG TACCAAAAAG ATGCAACGAC ATGATGAATG TTGGTCGACT CCAGGGGTTT
    GATGGAAAGA TTGTTGCTCA GGGGCGTCTC CTACTCCAGG ACACCTTCAT GGTGGCCGAA
    CCAGAGGGAG GCCTGCTGAA CAGGATGAAG GAGAGGAGAG TCTTCCTCTT TGAGCAGATC
    GTCATCTTCA GTGAACCTCT AGATAAAAAG AGGGGCTTCT CCATGCCTGG CTATTTGTAC
    AAATACAGCA TCAAGGTGAA CTGTCTGGGA CTTGAGGACA GTGTGGACGG AGACCCCTGT
    AAATTTGCAC TGACCTCCAG AACGTCCAAT AGCAGCAAAG ATGCCTTCAT TCTGCACTCG
    AGCCATCCTG GAGTACGACA AGTCTGGATG CTGCAGATCA GCCAAATCCT GGAGAGCCAA
    CGCAATTTCC TCAATGCACT TACATCTCCT ATAGACTACC AGAGGAACCA CGTGGAGGGT
    CCTGGTGTGT CAGGCAGCGG TGTACAGGCT GGAGGCAGCG GGGGGCAGAT GATGGCCCCT
    GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG GGTTCCCGTT CTCGTCCGTC CCGTATCCCC
    CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT CATCACTCTC CTGCCCTGGG GCCTGGAGCC
    CACGAGCATG ACGGCCCCGA CAAACTATCA GGTATGTCCC CAAGACCTCT CTCCAGGGGT
    CCCTCACCCT CATGCACCAC AGAGCCCGAG CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC
    CCCAAACAGA CGGACTCCCA ACAGACAGAA AGCCCAGCCA AAGAGATCCC ACGAGCCACT
    GTGGCCCCGC TGGCTCTTGT CAAACCCAGA CCTGGAACGG TTTCACCAAT GGCCTCTCCG
    TTAGCCACAC CGGCTTTCAA AGACTCCATT CCACCCTGTA GTCCAGGCCC GAAGACTGGC
    AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG TCCCCCGCCA GCAGGCCTGC TTCCTTCACC
    TTCCCCGGGG ACGCCTGCGA CACGCTGAGC CGACCAAATC ACAACCAGAG TCAGCGGCAC
    TCCACCCACA GTAAAGACGC AGACCGTATG AGCACCTGCT CCTCTACCAG TGAGCAGTCT
    ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA AGCAGCAGCA GCAGTAATAT CTCCACCATG
    TTGGTGACTC AGGACTATGT GGCTCTGAAA GAGGATGAGA TTAATGTGGG TCAGGGGGAG
    GTGGTGCAGA TCCTGGCCTC CAATCAGCAG AATATGTTCT TGGTGTTTCG TGCCGCTACT
    GAGCAGTGTC CTGCCGCCGA GGGCTGGATC CCCGGATACG TGCTGGGACA CACATCCGCC
    ATCATCCCTG ACGCTCCAGA CGGAACCATA AAGAAATCGT CATCATCATG GCACACATCC
    CTCCGTATTC GAAAGAAGTC AGAGAAAAGA GAAAAGGACA ATAAAAAAGA GGCCAAGCTG
    GAAAACGGCT ACCGGAAATC CAGAGAGGGA CTGACCAACA AGGTCTCTGT GAAGCTTCTA
    AATCCCAATT ATATATATGA TGTTCTCCCG GAGTTCCTCG TCCCGCTGAG TGACGTGACC
    TGTGATAAAG GCGAGTGTGT GACTCTGAGG TGTAAAGTGT GTGGTCGGCC CAAAGCCACG
    GTCACGTGGA AGGGACCTGA GCAGAAAACA CTCACCAACA ACGGCCACTT CAGCATCGCC
    TACAGTGAGA CTGGTGAGGC CACGCTGCGG ATCGTAGGTG TGACCTCAGA GGATGATGGC
    AGTTACACCT GCATTGCCAC GAATGACATT GGCAGTGTGG CATCATCAGC CAGTCTCAGA
    GTGCTGGGCA CGACCAGCGA TGGGATCCGG GTCATGTGGA AAGACAACTT TGAGTCCTTC
    TACACTGAGG TAGCAGAGCT TGGCAGGGGA CGGTTCTCTG TAGTGAAACG CTGTGACCAG
    AGGGGATCAA AGCGGACCGT GGCGGTCAAG TTTGTCAATA AGAAACTGAT GAAGCGAGAC
    CAGGTGACCC ATGAGTTCAG TGTCCTACAG AGACTCCAGC ACCCTCATCT GGTCAGACTG
    CTGGACACCT TCGAGACCTC CAGCAGCTAC GCACTGGTCC TGGAGATGTC TGATCAGGGC
    CGTCTGCTGG ACTACATCGT GAGCTGGGGG AATCTCACTG AGGAGAAAGT GGCCTTTTAC
    CTCCGGGACA TTTTAGAAGC TTTGCAGTAC CTGCACAACT GCAGAATAGT CCATCTGGAT
    CTAAAGCCTG AGAATCTGGT GGTGGAGCAG AGCCCGTCTC AGCCGCTGGT CAAGCTGACG
    GACTTCGGTG ATGCTGTGCA GCTCAACTCC ACGCCGTACG TTCACCCTCT GCTGGGGAGC
    CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC GGGGACCCTG TCTCTCTGTC CTCCGACCTG
    TGGAGTCTGG GGGTCCTGAC GTACGTCATG CTGAGCGGAG CATCTCCGTT CCTTGATGAG
    AGCGTGGAGG AGACCTGCCT CAACATCTGC CGCTTGGACT TCAGCTTTCC TGATGACTAC
    TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT TTTATGTGCC TGCTGCTCCG GATGGAGCCA
    TCCAAACGTC CACCAGCCAC CTCATGTCTA CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC
    CGACGCTCAG CCGAATGCAT CGATACCTCC AGACTGATCT CCTTCATCGA CCGACGCAAA
    CACCAGAACG ACCTGCGGCC TCTCACTGGC ATCAGAGCCT TCCTGCAGAC TCGATTACAG
    CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009292696.1
    CDS192..9443
    Translation

    Target ORF information:

    RefSeq Version XM_009294421.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio trio Rho guanine nucleotide exchange factor a (trioa), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294421.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGTACCTGC ACTGCGGTGC TGTTGAGGAC ACGGATTCGC TGTCCAGCTG GTGGAACGGC 
    AATGTGGCGT CACTTTTGGA CAACCACAGG TGGCAGTACG CACAGACGGG CAACACGCTG
    ATTCAGCTGC CAGGATTTCG CAGGAATGAA GAAATGAAGG CGATGGAGGT GTTGCCCATT
    TTAAAAGAAA AAGTGGCCTT CTTATCAGGG GGCAGAGACA AGCGTGGCGG TCCAGTTTTA
    ACATTCCCAT CACGAACCAA CCATGACCGA ATACGACACG AGGATCTCCG CAGACTCATC
    GCCTACCTCG CCGGCATCCC AAGCGAGGAT GTCTGCAAAC ATGGCTTCAC GGTCATCGTG
    GACATGCGTG GCTCCAAATG GGATTCCATC AAGCCTCTGC TGAAGATCCT CCAGGAGTCG
    TTCCCCTGCT GCATCCACAT CGCCCTCATC ATCAAACCAG ACAACTTCTG GCAGAAACAG
    AGGACCAATT TCGGCAGCTC CAAGTTTGAG TTTGAGACCA CAATGGTTTC TTTGGAGGGC
    TTATCGAAGG TGGTGGACCC ATCCCAGTTG ACGGCTGACT TTGAGGGCAG CCTGGACTAC
    AACCATGAAG AGTGGATTGA AATCCGCGTG GCCTTCGAAG ACTTTACAGG CAATGCTCGG
    CACCTGCTCG CCCGGCTAGA AGAGATGCAT GAAACGGTGA CGCGTAAAGA CTTACCGCAG
    GACTTAGATG GAGCCCGACG GATGATAGAG GAGCACGCGG CTTTGAAGAA GAGGGTGATA
    AAGGCTCCGG TGGAGGAGGT GGACAATGAG GGCCAAAGGC TCCTGCAAAG GATCCAGAGC
    AGTGAGAGCT ACGCCAACCG AACTGTGCCT GTGCCGCCTG GTCAGAGGGA AGGGCAGGGG
    CAGCCCAACG CCGACACCCA GGGCCTGGTG CCCCGTATTA CAGCTCTGCT GGAGAAACTG
    CACAGCACCA GGCAGAACCT GCACCAGTCC TGGCACATCC GCAAGCTGCA GCTGGACCAG
    TGCTTCCAGC TCAGGCTGTT CGAGCAGGAC GCTGAGAAGA TGTTTGATTG GATCATGCAC
    AATAAAGGGC TGTTTCTGGC TGGATACACT GAGATCGGCA ACAACCACCC TCACGCCATG
    GAGCTGCAGA CCCAGCACAA TCACTTCGCC ATGAACTGCA TGAATGTTTA CGTGAACATA
    AACCGCATCA TGTCTGTGGG GAACCGGCTG CTGGAGGCCG GTCATTACGC GTCGCAGCAG
    ATCAAGCAGA TCTCAGGTCA GCTGGAGCAG GAGTGGAAAG CTTTCGCCGC TGCGCTGGAC
    GAGAGGAGTG CTCTGCTGGA GATGTCTGCT ACCTTCCACC AGAAATGCGA CCAGTACATG
    AGTAATGTGG ACTCGTGGTG TAAAGCGTGT GGAGAAGTGG ATCTGCCCTC AGAGCTGCAG
    GAACTGGAGG ACGCTATACA CCATCACCAG GGCCTGTATG AACACATCAC CGCTGCCTAC
    TCGGAGGTGA GCCAAGACGG AAAAGCCTTA TTGGACAAAC TTCAGCGCCC CTTGACGCCC
    GGCAGTGCTG ATTCGCTGAC AGCTTCCGCC AATTACACCA AGGCGGTGAA CCATGTTTTG
    GACATCATCC ATGAGGTTCT GCATCACCAA AGGCAGCTGG AGAACATCTG GCAACATCGC
    AAGGTCCGGC TGCACCAGCG TCTGCAGCTG TGTGTTTTCC AGCAGGACGT TCAGCAGGTT
    CTGGACTGGA TTGAGAACCA TGGCGAGGCA TTTCTCAGCA AGCACACAGG GGTGGGAAAA
    TCTCTGCATC GCGCTCGAGC TCTGCAGAAA CGCCATGAAG ACTTTGAGGA AGTGGCTCAG
    AACACGTACA CAAATGCGGA TAAGCTTCTG GAAGCGGCAG AGCAGCTGGC CCAGACAGGC
    GAGTGTGACC CAGAGGAGAT CTATCAGGCC GCTCATCAGC TGGAGGACCG AATCCAGGAC
    TTTGTTCGGC GCGTGGAGCA GCGCAAGGTC CTGCTGGACA TGTCAGTGGC TTTCCACACT
    CACGTCAAAG AGTTGTGGAC GTGGCTGGAG GAGCTGCAGA AGGAGTTGCT GGACGATGTG
    TACGCCGAGT CTGTGGAAGC CGTGCAGGAT CTGATCAAGC GTTTCGGGCA GCAGCAACAA
    ACCACACTGC AGTTCACTGT AAACGTCATC AAAGAAGGAG AAGATCTCAT CCAGCAGCTC
    AGAGACCTGG CCATCTCCAG CAACAAGACG CCTCACAACA GCTCCATCAA CCACATCGAG
    AGCGTCCTGC AGCAGCTGGA TGAGGCTCAG GCTCAGATGG AGGAGCTTTT CCAGGAGAGG
    AAGATCAAGC TGGAGCTTTT CCTGCAGCTC AGGATCTTTG AGCGGGACGC CATAGATATT
    ATCTCAGATC TGGACTCGTG GAATGAGGAG CTGTCGCAGC AGATGAATGA GTTTGATACA
    GAAGACCTGA CGCTGGCTGA ACAGAGACTG CAGCACCATG CTGATAAAGC CCTGACCATG
    AACAACCTGG CCTTCCACGT CATCCACCAG GGCCAGGAAC TGCTGCAGTA CGTCAACGAG
    GTGCAGGCGT CTGGTGTGGA GTTGCTGTGT GACCGTGATG TGGACATGGC CACCCGTGTT
    CAGGACCTGC TAGAGTTTCT ACATGAGAAA CAGCAAGAGC TGGACGTGGC TGCAGAACAG
    CATCGCAGAC ACCTTGAGCA GTGTGTGCAA CTGCGCCACC TACAAGCCGA AGTCAAACAG
    GTTCTGGGTT GGATCCGTAA TGGCGAGTCG ATGCTGAATG CAGGTCTGAT TACGGCCAGC
    TCTCTTCAAG AAGCAGAGCA GTTGCAGAGA GAGCATGAGC AATTCCAGCA TGCTATCGAG
    AAAACCCATC AGAGCGCCCT GCAGGTCCAG CAGAAAGCCG AAGCCCTGCT GCAGGCCAAT
    CACTACGACA TGGACATGAT TCGAGACTGC GCGGAGAACG TGGCTTCCCA CTGGCAGCAG
    CTCATGCTGA AGATGGAGGA CCGGCTCAAG CTGGTCAACG CCTCCGTGGC CTTCTATAAG
    ACCTCTGAAC AGGTTTGCAG TGTTCTGGAG AGTCTGGAGC AGGAGTACAA GCGTGAGGAG
    GACTGGTGCG GAGGAGCTGA CAAACTGGGA CCCAACTGTG AATCGGACCA CGTCACACCC
    ATGATCAGCA AACACCTGGA GCAGAAAGAG GCTTTCCTCA AGGCCTGCAC GTTGGCCAGG
    CGTAATGCAG ACGTCTTCCT CAAATACATG CACAGGAACA GCGTCAGCAT GCCCGGGATG
    TTGAGTCACG TCAAAGCTCC AGAGCAGCAG GTCAAAAATA TCCTGAATGA GCTGCTGCAG
    AGGGAGAACC GCGTGCTGCA CTTCTGGACT ATGAGAAAGA GGAGGCTGGA CCAGTGTCAG
    CAATACGTGG TGTTTGAACG CAGCGCCAAA CAGGCTCTGG AGTGGATCCA CGACACTGGC
    GAGTTTTATC TGTCCACACA CACGTCCACC GGCTCCAGCA TACACCACAC ACAGGAGCTG
    CTGAAGGAGC ACGAGGACTT CCACATCACA GCCAAGCAAA CCAAAGAACG GGTGAAGCTG
    CTGATCCAGC TGGCAGATGG TTTCTGTGAT AAGGGTCACT CTCACGCCGC TGAGATTAAG
    AAATGGGTGA CAGCGGTGGA CAAACGCTAT CGAGATTTCT CGCTCCGCAT GGACAAGTAC
    CGCACATCTC TGGAGAAAGC ACTCGGCATC TCCTCAGACT CCAATAAAGC TAGCAAAGAC
    TTGCAGCTGG ACATCATCCC TGCCAGTGCT CCGGGGTCAG AGGTCAAACT CAGAGACGCT
    GCTCATGAGC TCAATGAAGA GAAGCGCAAG TCTGCCCGCA GGAAGGACTT CATCATGGCT
    GAGCTGATCC AGACAGAAAA GGCCTATGTG CGAGACTTGA GAGAGTGCAT GGACACGTAT
    CTGTGGGAGA TGACCAGCGG CGTGGAGGAG ATCCCACCAG GCATCGTCAA CAAAGAGCAC
    ATCATTTTTG GTAACATGCA AGATCTGTAC GAGTTCCATC ACAATATATT CCTCAAAGAG
    CTGGAGAAAT ACGAGCAGCT TCCTGAAGAT GTGGGCCACT GTTTTGTGAC TTGGGCTGAT
    AAGTTCCAGA TGTATGTGAA CTACTGCAAG AACAAACCAG ACTCCACTCA GCTTATTCTT
    GAACATGCCG GAGGATACTT CGACGAGATC CAGCAGAGGC ATCGCCTGGC CAACTCCATC
    TCCTCATACC TCATCAAACC TGTTCAGAGA ATAACCAAGT ACCAGCTGCT CCTCAAGGAG
    CTCCTGACGT GCTGCGAGGA AGGCAAAGGT GAAATTAAAG ATGGCCTGGA GGTGATGCTC
    AGTGTTCCCA AAAGAGCCAA CGACGCCATG CACTTGAGCA TGCTTGAAGG GTTTGATGAG
    AACATCGAGT CCCAGGGAGA GCTGATCCTG CAGGAGGCGT TTCAGGTGTG GGATCCCAAA
    ACTCTGATCC GCAAGGGAAG AGAACGACAC CTGTTCCTCT TCGAGATGTC ACTGATCTTC
    AGCAAGGAGG TCAAAGACTC CAATGGCAGG AGCAAGTACA TCTACAAGAG CAAGCTTTTC
    ACCTCTGAAC TGGGTGTCAC TGAGCATGTG GAGGGAGATC CATGCAAATT TGCTCTCTGG
    GTTGGACGAA CCCCAACTTC TGACAACAAG ATTGTCCTCA AGGCGTCCGG TATTGAGAAC
    AAACAGGACT GGATCAAGCA CATCCGGGAA GTGATTCAGG AGCGCACCGT TCACCTGAAG
    GGGGCGCTGA AAGAGCCCAT TCACATCCCC AAAGCCTCCA CAGCCAAACA CAAGGGGCGC
    AGGTACACCC CAAGGGATGG AGAGGATCTG GACAGTCAGG GTGACGGCAG CAGTCAACCA
    GACACCATCT CACTGGCCTC CAGGACCTCA CAGAACACAC TGGACAGCGA CAAGTTGTCT
    GGTGGCTGTG AGCTGACGGT GGTCATCCAT GACTTTATGG CCGGTAACAG TAATGAGCTG
    ACCATCCGGA GAGGACAGAC GGTGGAGGTG CTCGAGAGAC TTCATGATAA GCCGGACTGG
    TGTTTGGTTC GCACCACAGA CCGTTCGCCG GCGCTGGAGG GCCTGGTGCC CTGTGCCATG
    CTCTGTATCG CCCACTCCCG CAGCAGCATG GAGATGGAGG GCATCTTCAA CCACAAAGAT
    GCACTCTCTG TATGCAGTAA TGATGCCATC ATGCCAGGCT CCTCTGCCAC CCTGCAGCCG
    GGTCATGTGA TGGGTTCCCA GAGTTCTCCT GGACCTAAAA GGCCTGGCAA CACATTAAGG
    AAATGGCTGA CCAGTCCTGT GAGGAGACTG AGCAGCGGTA AAGCTGACGG ACACGTCAAG
    AAGCTGGCAC ACAAACACAA GAAGAGCCGT GACGTCAGAA AGAGCGCAGA CGCCGGTTCA
    CAGAAAGACT CGGACGACAG CGCTGCGACG CCACAAGATG AGACCCTCGA GGAGAGGGTG
    CGTAATGAGG GTCTGAGCAG CGGCACGCTC TCCAAATCCT CCTCTTCAGG GATGCAGAGC
    TGTGGAGAGG AAGAGGGAGA GGAAGGAGCT GACGCTGTGC CTCTTCCTCC ACCAATGGCC
    ATCCAGCAGC ACAGCCTCCT CCAGCCGGAT GCGCAGGATG ACAAGACGTC TTCACGTCTT
    TCCGTCAGAC CCTCCAGTTC TGAGACCCCC AGCGCTGCAG AGCTCGTCAG TGCCATTGAG
    GAGCTGGTCA AAAGCAAGAT GGCTCTGGAA GACAGACCCA GCACTCTGTC AGTGGAGCAG
    GGCGACAGCA GCAGCCCTTC ATTCAACCCA TCTGATAACT CTCTCCTGTC CTCCTCCTCT
    CCAATCGATG AGATAGAGGA ACGCAAGACC GGTTTCTTCA AGAAGCGCCA TTACGTCCTG
    CTGGAGCTGA TCGAGACGGA GAGAGACTAT GTGCGAGATC TCAGTTTGGT CGTGGAGGGC
    TACATGGCCA GGATGAGGGA GGACGGGGTT CCAGATGATA TGAAAGGAAA AGACAAGATC
    GTTTTTGGAA ACATTCAGCA GATATATGAC TGGCACAAAG ATTTCTTCCT GGGAGAGCTT
    GAGAAGTGCC TTGAGGACCC TGACCGGCTG GGCCCTCTCT TCCTCAAACA TGAGCGGAGA
    CTGCACATGT ACATTGTTTA CTGCCAGAAC AAACCTAAAT CTGAACACAT CGTCTCAGAG
    TACATCGACA CATATTTTGA GGATCTGAAG CAGCGTTTGG GTCACAGGCT TCAGATCACT
    GATCTGCTCA TTAAACCTGT ACAGAGAATC ATGAAGTACC AGCTGCTGCT GAAGGATTTC
    CTCAAATTTT CCAAAAAAAT CGGAACAGAT TCGATTGAAT TAGAGAAAGC GGTTGAGGTC
    ATGTGCATTG TACCAAAAAG ATGCAACGAC ATGATGAATG TTGGTCGACT CCAGGGGTTT
    GATGGAAAGA TTGTTGCTCA GGGGCGTCTC CTACTCCAGG ACACCTTCAT GGTGGCCGAA
    CCAGAGGGAG GCCTGCTGAA CAGGATGAAG GAGAGGAGAG TCTTCCTCTT TGAGCAGATC
    GTCATCTTCA GTGAACCTCT AGATAAAAAG AGGGGCTTCT CCATGCCTGG CTATTTGTAC
    AAATACAGCA TCAAGGTGAA CTGTCTGGGA CTTGAGGACA GTGTGGACGG AGACCCCTGT
    AAATTTGCAC TGACCTCCAG AACGTCCAAT AGCAGCAAAG ATGCCTTCAT TCTGCACTCG
    AGCCATCCTG GAGTACGACA AGTCTGGATG CTGCAGATCA GCCAAATCCT GGAGAGCCAA
    CGCAATTTCC TCAATGCACT TACATCTCCT ATAGACTACC AGAGGAACCA CGTGGAGGGT
    CCTGGTGTGT CAGGCAGCGG TGTACAGGCT GGAGGCAGCG GGGGGCAGAT GATGGCCCCT
    GGAGGAGGAG TAGGGGTCCC GGCTGGGCCG GGTTCCCGTT CTCGTCCGTC CCGTATCCCC
    CAGCCCTCGC GCTTGCCTCA ACCGCTCCGT CATCACTCTC CTGCCCTGGG GCCTGGAGCC
    CACGAGCATG ACGGCCCCGA CAAACTATCA GGTATGTCCC CAAGACCTCT CTCCAGGGGT
    CCCTCACCCT CATGCACCAC AGAGCCCGAG CCAAAGGTGA AGCTTCCTGC GAGCCCTCAC
    CCCAAACAGA CGGACTCCCA ACAGACAGAA AGCCCAGCCA AAGAGATCCC ACGAGCCACT
    GTGGCCCCGC TGGCTCTTGT CAAACCCAGA CCTGGAACGG TTTCACCAAT GGCCTCTCCG
    TTAGCCACAC CGGCTTTCAA AGACTCCATT CCACCCTGTA GTCCAGGCCC GAAGACTGGC
    AGCAGCTCGT TTTGGAGCTC AGTGCCCGCG TCCCCCGCCA GCAGGCCTGC TTCCTTCACC
    TTCCCCGGGG ACGCCTGCGA CACGCTGAGC CGACCAAATC ACAACCAGAG TCAGCGGCAC
    TCCACCCACA GTAAAGACGC AGACCGTATG AGCACCTGCT CCTCTACCAG TGAGCAGTCT
    ATCCAGTCCA CTCAGAGCAA TGGGAGTGAA AGCAGCAGCA GCAGTAATAT CTCCACCATG
    TTGGTGACTC AGGACTATGT GGCTCTGAAA GAGGATGAGA TTAATGTGGG TCAGGGGGAG
    GTGGTGCAGA TCCTGGCCTC CAATCAGCAG AATATGTTCT TGGTGTTTCG TGCCGCTACT
    GAGCAGTGTC CTGCCGCCGA GGGCTGGATC CCCGGATACG TGCTGGGACA CACATCCGCC
    ATCATCCCTG ACGCTCCAGA CGGAACCATA AAGAAATCGT CATCATCATG GCACACATCC
    CTCCGTATTC GAAAGAAGTC AGAGAAAAGA GAAAAGGACA ATAAAAAAGA GGCCAAGCTG
    GAAAACGGCT ACCGGAAATC CAGAGAGGGA CTGACCAACA AGGTCTCTGT GAAGCTTCTA
    AATCCCAATT ATATATATGA TGTTCTCCCG GAGTTCCTCG TCCCGCTGAG TGACGTGACC
    TGTGATAAAG GCGAGTGTGT GACTCTGAGG TGTAAAGTGT GTGGTCGGCC CAAAGCCACG
    GTCACGTGGA AGGGACCTGA GCAGAAAACA CTCACCAACA ACGGCCACTT CAGCATCGCC
    TACAGTGAGA CTGGTGAGGC CACGCTGCGG ATCGTAGGTG TGACCTCAGA GGATGATGGC
    AGTTACACCT GCATTGCCAC GAATGACATT GGCAGTGTGG CATCATCAGC CAGTCTCAGA
    GTGCTGGGCA CGACCAGCGA TGGGATCCGG GTCATGTGGA AAGACAACTT TGAGTCCTTC
    TACACTGAGG TAGCAGAGCT TGGCAGGGGA CGGTTCTCTG TAGTGAAACG CTGTGACCAG
    AGGGGATCAA AGCGGACCGT GGCGGTCAAG TTTGTCAATA AGAAACTGAT GAAGCGAGAC
    CAGGTGACCC ATGAGTTCAG TGTCCTACAG AGACTCCAGC ACCCTCATCT GGTCAGACTG
    CTGGACACCT TCGAGACCTC CAGCAGCTAC GCACTGGTCC TGGAGATGTC TGATCAGGGC
    CGTCTGCTGG ACTACATCGT GAGCTGGGGG AATCTCACTG AGGAGAAAGT GGCCTTTTAC
    CTCCGGGACA TTTTAGAAGC TTTGCAGTAC CTGCACAACT GCAGAATAGT CCATCTGGAT
    CTAAAGCCTG AGAATCTGGT GGTGGAGCAG AGCCCGTCTC AGCCGCTGGT CAAGCTGACG
    GACTTCGGTG ATGCTGTGCA GCTCAACTCC ACGCCGTACG TTCACCCTCT GCTGGGGAGC
    CCGGAGTTCG CAGCCCCTGA GCTGGTCCTC GGGGACCCTG TCTCTCTGTC CTCCGACCTG
    TGGAGTCTGG GGGTCCTGAC GTACGTCATG CTGAGCGGAG CATCTCCGTT CCTTGATGAG
    AGCGTGGAGG AGACCTGCCT CAACATCTGC CGCTTGGACT TCAGCTTTCC TGATGACTAC
    TTCCAGGGAG TGAGTCAGGC GGCGCGTGAT TTTATGTGCC TGCTGCTCCG GATGGAGCCA
    TCCAAACGTC CACCAGCCAC CTCATGTCTA CAGGAGCCCT GGTTGAGAGC CGGCGGGGGC
    CGACGCTCAG CCGAATGCAT CGATACCTCC AGACTGATCT CCTTCATCGA CCGACGCAAA
    CACCAGAACG ACCTGCGGCC TCTCACTGGC ATCAGAGCCT TCCTGCAGAC TCGATTACAG
    CCCAGGATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.