trio cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	trio
Entrez Gene ID	43974
Full Name	CG18214 gene product from transcript CG18214-RA
Synonyms	0368/10,0959/14,1372/03,1386/06,BEST:LD36950,CG18214,CG9208,DTrio,Dmel\CG18214,M89,TRIO,Trio,UNC-73/Trio,dTrio,l(3)036810,l(3)6D104,l(3)S036810,l(3)S095914,l(3)S137203,l(3)S138606,l(3)S[1372/3],l(3)S[1386/06],l(3)trio
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61E1-61E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_143703.3	NP_651960.2	trio, isoform A
NM_206228.2	NP_995950.1	trio, isoform F
NM_167848.2	NP_728561.1	trio, isoform D
NM_167850.2	NP_728563.1	trio, isoform E
NM_167847.2	NP_728560.1	trio, isoform C
NM_167849.2	NP_728562.1	trio, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIO	NP_009049.2
	TRIO	XP_002804369.1
Canis lupus familiaris (dog)	TRIO	XP_005639720.1
Mus musculus (house mouse)	Trio	NP_001074771.1
Danio rerio (zebrafish)	trioa	NP_001097996.1
Rattus norvegicus (Norway rat)	Trio	XP_003749274.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006107	XP_316167.4
Gallus gallus (chicken)	TRIO	XP_419004.4
Pan troglodytes (chimpanzee)	TRIO	XP_517637.3
Caenorhabditis elegans (roundworm)	unc-73	NP_001021496.1
Xenopus tropicalis (tropical clawed frog)	trio	NP_001123393.1
Drosophila melanogaster (fruit fly)	trio	NP_651960.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results have shown that trio expression in the class IV dendritic arborization sensory neurons rescues the blocked larval-pupal transition phenotype in seq-enhanced trio mutant larvae.
Title: The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses.

Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitor
Title: Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitors.

allele of sequoia dominantly enhances a trio mutant phenotype to influence Drosophila larval behavior
Title: An allele of sequoia dominantly enhances a trio mutant phenotype to influence Drosophila larval behavior.

the primary function of Syd-1 in R7 photoreceptors may be to promote Trio activity.
Title: Loss of syd-1 from R7 neurons disrupts two distinct phases of presynaptic development.

Trio shapes the complexity of dendritic arbors and does so in a way that mimics the effects of its target, Rac.
Title: Control of dendritic morphogenesis by Trio in Drosophila melanogaster.

The following trio gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trio cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19346	NM_206228.2 Latest version!	Drosophila melanogaster trio, transcript variant F (trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19346	NM_167849.2 Latest version!	Drosophila melanogaster trio, transcript variant B (trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19347	NM_167850.2 Latest version!	Drosophila melanogaster trio, transcript variant E (trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19345	NM_167848.2 Latest version!	Drosophila melanogaster trio, transcript variant D (trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa19344	NM_167847.2 Latest version!	Drosophila melanogaster trio, transcript variant C (trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa19344	NM_143703.3 Latest version!	Drosophila melanogaster trio, transcript variant A (trio), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trio ( NM_206228.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995950.1 Drosophila melanogaster trio, transcript variant F (trio), mRNA.

Price & Availability↑

CloneID	OFa19346
Clone ID Related Accession (Same CDS sequence)	NM_206228.2 , NM_167849.2
Accession Version	NM_206228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trio, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_206228.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCAACA ACAACAACAA CAATAACAGT GACTGTGGCC ATAGGAGCCG CCTAGATAAG 
ATGGCCGAAA ACGGTTTCCA CTACAAAAGT GCTAGCGCGG TGCCAGGAAC CACCGTTTGC 
TGCGATAACA ACAACGAGGC TTCGAATCCT CATCCAGAGC CAAACTATAA CTCGCTGAAA 
TTGAAACGCA GCTCGGCTCC CGCACATTCC CGACCCAAAT CGATGCACAA GTTGTTCAAA 
TCTATATCGC TGAGGGTCGG GGATAATGGA AATGCAAATG GAAACGGGAA AGGGAACGGG 
AACGGGAACG GGAACGCCGC CGTTTTGCAC CACCAGCAAT CCCTGGGTGG CCAACCATCG 
GGATTCACAT GTGTGTGCTG CGAGGAGGTG ACCGAGATCA GGACGGAAAA CCCCAAGAGG 
CGCAACTCCT TTTTGAAGGA ACTCAAGAAA CGACGCAACT GGCTGCCGCT GATGAATCGC 
AAGGGAACGG ACAAGCCGCC AAGTAGCAAA CCGCTGGTGA AGAAGCCCTC GGAGAAAAAC 
CTGAGAACAC CCCAGAAGCA CGCCGAGGAG CTGGCCGAGC AGCAAAACCA GCAGCCAGGT 
GCCAGTCCCG CCACCGTGCT GCCATCGTCG CAATTCCCCA GCTCAACTCA GCATCCAGGA 
GGAGCAGGAG CCGGCGACTA TGAGCCGGAC GAGGAGGTCG GCCTCGAGCT GCCGCCGCCC 
ATGAAACCCA TCCAAGAGCC GCACCTGATT GCCAACGGAC CGCCCGCCTT TGCCAAGGAC 
GCCAAGGAGA GCTCGGGCAA CATGGCCAGC CCCGGCAAAA TGGACGGCAA TCCACTTTCG 
GAAATAGAAG AAATTGTCAA GACGACAACG GAGCAGCACG AGTCCAACAG TCGCGTGGAT 
GGAGGCGGAG GTGTGGAAAA TGCCAGCACC AACGGCCATG GTCGATCCCA CGACAACAAT 
GATGAGAGCC ACACCGCTGT GTCTGACAAG GCTGCAGCCC TCAAAAAGCG GCAATGCGTC 
TTTGCGGAGC TGATGTCCAC GGAGGAGGCA TATGTACAGG ATCTGCATGA GATCGTCAAT 
GGTTACATGA CAGAGATTAA CAACACGAAC AGTGATATAC CCATGCCTGA GGACCTGAAG 
GGTGGTAAAA TGAAGTTGGT CTTCAACAAT ATTAAAGACA TCTACGAGTG GCACAGGGAC 
TTCTTCCTGA GAGCCCTGCG CAACTGCCAA AAATCGCCGG CGGACCTGGG TCCGCTCATC 
AAGCGGTCAG CCACCAAGTT CGCTCTGTAC TACACCTATT GCAGCAACAA ACCGCTGTCC 
GAATACATAG TCAGTGCCCA TTATCAGTAT TTCGATAGCA TACGTCAGAA GCTGGGACAT 
CGTCTGGATC TGAGTAACTT GATCATCAAG CCGGTGCAGC GAATCACTAA ATACGAGCTA 
CTCATCAAGG AGATCATCAA GGCCACCGAG GGAGCCGGGC TTTACAAGGA GGTGCCCATG 
CTGCAGGAGG CCTACCAACA GATGAAGGTC GTCGTCAAGA CGGTCAACGA TATGATGGTG 
GTGCTGCGCA GCTTGCAGGA CTTCGATGGG GAGATAACCG CCCAAGGCAG CCTGTTGATG 
CAGGGACCCA TGAACTGCGT GGTGGATGCG GCACAGAAGC ACCGCGAGCT TCAGGTGTTC 
CTCTTCCAGC AGATCATCAT CTTTGCGGAT ATTGAGAAGA CCAAGAACCA GTATGCCAGT 
CCCATATTCA AATACAGATC GCACATACAG CTCAATCACA TGCAAATGAA GGAACTGGGC 
GACTGCCGCT TTCAAATAAG GTCGACTGAC CCCAAGATCC CAGAGATGAC GATCATCTGC 
CAGGCGGCGT CGCAGGAGAA CTACGCGGGG TGGCGCGATA TGCTAAACAA GATACTGCAG 
CAGCAGAACG ACCTGATCTT CATGCTCTCC AATCCCCTGT CAACCAAAAA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995950.1
CDS	108..2084
Translation	MCNNNNNNNSDCGHRSRLDKMAENGFHYKSASAVPGTTVCCDNNNEASNPHPEPNYNSLKLKRSSAPAHSRPKSMHKLFKSISLRVGDNGNANGNGKGNGNGNGNAAVLHHQQSLGGQPSGFTCVCCEEVTEIRTENPKRRNSFLKELKKRRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLMQGPMNCVVDAAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQMKELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPLSTKNK

Target ORF information:

RefSeq Version	NM_206228.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trio, transcript variant F (trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206228.2

ORF Insert Sequence:

ATGTGCAACA ACAACAACAA CAATAACAGT GACTGTGGCC ATAGGAGCCG CCTAGATAAG 
ATGGCCGAAA ACGGTTTCCA CTACAAAAGT GCTAGCGCGG TGCCAGGAAC CACCGTTTGC 
TGCGATAACA ACAACGAGGC TTCGAATCCT CATCCAGAGC CAAACTATAA CTCGCTGAAA 
TTGAAACGCA GCTCGGCTCC CGCACATTCC CGACCCAAAT CGATGCACAA GTTGTTCAAA 
TCTATATCGC TGAGGGTCGG GGATAATGGA AATGCAAATG GAAACGGGAA AGGGAACGGG 
AACGGGAACG GGAACGCCGC CGTTTTGCAC CACCAGCAAT CCCTGGGTGG CCAACCATCG 
GGATTCACAT GTGTGTGCTG CGAGGAGGTG ACCGAGATCA GGACGGAAAA CCCCAAGAGG 
CGCAACTCCT TTTTGAAGGA ACTCAAGAAA CGACGCAACT GGCTGCCGCT GATGAATCGC 
AAGGGAACGG ACAAGCCGCC AAGTAGCAAA CCGCTGGTGA AGAAGCCCTC GGAGAAAAAC 
CTGAGAACAC CCCAGAAGCA CGCCGAGGAG CTGGCCGAGC AGCAAAACCA GCAGCCAGGT 
GCCAGTCCCG CCACCGTGCT GCCATCGTCG CAATTCCCCA GCTCAACTCA GCATCCAGGA 
GGAGCAGGAG CCGGCGACTA TGAGCCGGAC GAGGAGGTCG GCCTCGAGCT GCCGCCGCCC 
ATGAAACCCA TCCAAGAGCC GCACCTGATT GCCAACGGAC CGCCCGCCTT TGCCAAGGAC 
GCCAAGGAGA GCTCGGGCAA CATGGCCAGC CCCGGCAAAA TGGACGGCAA TCCACTTTCG 
GAAATAGAAG AAATTGTCAA GACGACAACG GAGCAGCACG AGTCCAACAG TCGCGTGGAT 
GGAGGCGGAG GTGTGGAAAA TGCCAGCACC AACGGCCATG GTCGATCCCA CGACAACAAT 
GATGAGAGCC ACACCGCTGT GTCTGACAAG GCTGCAGCCC TCAAAAAGCG GCAATGCGTC 
TTTGCGGAGC TGATGTCCAC GGAGGAGGCA TATGTACAGG ATCTGCATGA GATCGTCAAT 
GGTTACATGA CAGAGATTAA CAACACGAAC AGTGATATAC CCATGCCTGA GGACCTGAAG 
GGTGGTAAAA TGAAGTTGGT CTTCAACAAT ATTAAAGACA TCTACGAGTG GCACAGGGAC 
TTCTTCCTGA GAGCCCTGCG CAACTGCCAA AAATCGCCGG CGGACCTGGG TCCGCTCATC 
AAGCGGTCAG CCACCAAGTT CGCTCTGTAC TACACCTATT GCAGCAACAA ACCGCTGTCC 
GAATACATAG TCAGTGCCCA TTATCAGTAT TTCGATAGCA TACGTCAGAA GCTGGGACAT 
CGTCTGGATC TGAGTAACTT GATCATCAAG CCGGTGCAGC GAATCACTAA ATACGAGCTA 
CTCATCAAGG AGATCATCAA GGCCACCGAG GGAGCCGGGC TTTACAAGGA GGTGCCCATG 
CTGCAGGAGG CCTACCAACA GATGAAGGTC GTCGTCAAGA CGGTCAACGA TATGATGGTG 
GTGCTGCGCA GCTTGCAGGA CTTCGATGGG GAGATAACCG CCCAAGGCAG CCTGTTGATG 
CAGGGACCCA TGAACTGCGT GGTGGATGCG GCACAGAAGC ACCGCGAGCT TCAGGTGTTC 
CTCTTCCAGC AGATCATCAT CTTTGCGGAT ATTGAGAAGA CCAAGAACCA GTATGCCAGT 
CCCATATTCA AATACAGATC GCACATACAG CTCAATCACA TGCAAATGAA GGAACTGGGC 
GACTGCCGCT TTCAAATAAG GTCGACTGAC CCCAAGATCC CAGAGATGAC GATCATCTGC 
CAGGCGGCGT CGCAGGAGAA CTACGCGGGG TGGCGCGATA TGCTAAACAA GATACTGCAG 
CAGCAGAACG ACCTGATCTT CATGCTCTCC AATCCCCTGT CAACCAAAAA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trio ( NM_167849.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728562.1 Drosophila melanogaster trio, transcript variant B (trio), mRNA.

Price & Availability↑

CloneID	OFa19346
Clone ID Related Accession (Same CDS sequence)	NM_206228.2 , NM_167849.2
Accession Version	NM_167849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trio, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_167849.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCAACA ACAACAACAA CAATAACAGT GACTGTGGCC ATAGGAGCCG CCTAGATAAG 
ATGGCCGAAA ACGGTTTCCA CTACAAAAGT GCTAGCGCGG TGCCAGGAAC CACCGTTTGC 
TGCGATAACA ACAACGAGGC TTCGAATCCT CATCCAGAGC CAAACTATAA CTCGCTGAAA 
TTGAAACGCA GCTCGGCTCC CGCACATTCC CGACCCAAAT CGATGCACAA GTTGTTCAAA 
TCTATATCGC TGAGGGTCGG GGATAATGGA AATGCAAATG GAAACGGGAA AGGGAACGGG 
AACGGGAACG GGAACGCCGC CGTTTTGCAC CACCAGCAAT CCCTGGGTGG CCAACCATCG 
GGATTCACAT GTGTGTGCTG CGAGGAGGTG ACCGAGATCA GGACGGAAAA CCCCAAGAGG 
CGCAACTCCT TTTTGAAGGA ACTCAAGAAA CGACGCAACT GGCTGCCGCT GATGAATCGC 
AAGGGAACGG ACAAGCCGCC AAGTAGCAAA CCGCTGGTGA AGAAGCCCTC GGAGAAAAAC 
CTGAGAACAC CCCAGAAGCA CGCCGAGGAG CTGGCCGAGC AGCAAAACCA GCAGCCAGGT 
GCCAGTCCCG CCACCGTGCT GCCATCGTCG CAATTCCCCA GCTCAACTCA GCATCCAGGA 
GGAGCAGGAG CCGGCGACTA TGAGCCGGAC GAGGAGGTCG GCCTCGAGCT GCCGCCGCCC 
ATGAAACCCA TCCAAGAGCC GCACCTGATT GCCAACGGAC CGCCCGCCTT TGCCAAGGAC 
GCCAAGGAGA GCTCGGGCAA CATGGCCAGC CCCGGCAAAA TGGACGGCAA TCCACTTTCG 
GAAATAGAAG AAATTGTCAA GACGACAACG GAGCAGCACG AGTCCAACAG TCGCGTGGAT 
GGAGGCGGAG GTGTGGAAAA TGCCAGCACC AACGGCCATG GTCGATCCCA CGACAACAAT 
GATGAGAGCC ACACCGCTGT GTCTGACAAG GCTGCAGCCC TCAAAAAGCG GCAATGCGTC 
TTTGCGGAGC TGATGTCCAC GGAGGAGGCA TATGTACAGG ATCTGCATGA GATCGTCAAT 
GGTTACATGA CAGAGATTAA CAACACGAAC AGTGATATAC CCATGCCTGA GGACCTGAAG 
GGTGGTAAAA TGAAGTTGGT CTTCAACAAT ATTAAAGACA TCTACGAGTG GCACAGGGAC 
TTCTTCCTGA GAGCCCTGCG CAACTGCCAA AAATCGCCGG CGGACCTGGG TCCGCTCATC 
AAGCGGTCAG CCACCAAGTT CGCTCTGTAC TACACCTATT GCAGCAACAA ACCGCTGTCC 
GAATACATAG TCAGTGCCCA TTATCAGTAT TTCGATAGCA TACGTCAGAA GCTGGGACAT 
CGTCTGGATC TGAGTAACTT GATCATCAAG CCGGTGCAGC GAATCACTAA ATACGAGCTA 
CTCATCAAGG AGATCATCAA GGCCACCGAG GGAGCCGGGC TTTACAAGGA GGTGCCCATG 
CTGCAGGAGG CCTACCAACA GATGAAGGTC GTCGTCAAGA CGGTCAACGA TATGATGGTG 
GTGCTGCGCA GCTTGCAGGA CTTCGATGGG GAGATAACCG CCCAAGGCAG CCTGTTGATG 
CAGGGACCCA TGAACTGCGT GGTGGATGCG GCACAGAAGC ACCGCGAGCT TCAGGTGTTC 
CTCTTCCAGC AGATCATCAT CTTTGCGGAT ATTGAGAAGA CCAAGAACCA GTATGCCAGT 
CCCATATTCA AATACAGATC GCACATACAG CTCAATCACA TGCAAATGAA GGAACTGGGC 
GACTGCCGCT TTCAAATAAG GTCGACTGAC CCCAAGATCC CAGAGATGAC GATCATCTGC 
CAGGCGGCGT CGCAGGAGAA CTACGCGGGG TGGCGCGATA TGCTAAACAA GATACTGCAG 
CAGCAGAACG ACCTGATCTT CATGCTCTCC AATCCCCTGT CAACCAAAAA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728562.1
CDS	108..2084
Translation	MCNNNNNNNSDCGHRSRLDKMAENGFHYKSASAVPGTTVCCDNNNEASNPHPEPNYNSLKLKRSSAPAHSRPKSMHKLFKSISLRVGDNGNANGNGKGNGNGNGNAAVLHHQQSLGGQPSGFTCVCCEEVTEIRTENPKRRNSFLKELKKRRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLMQGPMNCVVDAAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQMKELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPLSTKNK

Target ORF information:

RefSeq Version	NM_167849.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trio, transcript variant B (trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167849.2

ORF Insert Sequence:

ATGTGCAACA ACAACAACAA CAATAACAGT GACTGTGGCC ATAGGAGCCG CCTAGATAAG 
ATGGCCGAAA ACGGTTTCCA CTACAAAAGT GCTAGCGCGG TGCCAGGAAC CACCGTTTGC 
TGCGATAACA ACAACGAGGC TTCGAATCCT CATCCAGAGC CAAACTATAA CTCGCTGAAA 
TTGAAACGCA GCTCGGCTCC CGCACATTCC CGACCCAAAT CGATGCACAA GTTGTTCAAA 
TCTATATCGC TGAGGGTCGG GGATAATGGA AATGCAAATG GAAACGGGAA AGGGAACGGG 
AACGGGAACG GGAACGCCGC CGTTTTGCAC CACCAGCAAT CCCTGGGTGG CCAACCATCG 
GGATTCACAT GTGTGTGCTG CGAGGAGGTG ACCGAGATCA GGACGGAAAA CCCCAAGAGG 
CGCAACTCCT TTTTGAAGGA ACTCAAGAAA CGACGCAACT GGCTGCCGCT GATGAATCGC 
AAGGGAACGG ACAAGCCGCC AAGTAGCAAA CCGCTGGTGA AGAAGCCCTC GGAGAAAAAC 
CTGAGAACAC CCCAGAAGCA CGCCGAGGAG CTGGCCGAGC AGCAAAACCA GCAGCCAGGT 
GCCAGTCCCG CCACCGTGCT GCCATCGTCG CAATTCCCCA GCTCAACTCA GCATCCAGGA 
GGAGCAGGAG CCGGCGACTA TGAGCCGGAC GAGGAGGTCG GCCTCGAGCT GCCGCCGCCC 
ATGAAACCCA TCCAAGAGCC GCACCTGATT GCCAACGGAC CGCCCGCCTT TGCCAAGGAC 
GCCAAGGAGA GCTCGGGCAA CATGGCCAGC CCCGGCAAAA TGGACGGCAA TCCACTTTCG 
GAAATAGAAG AAATTGTCAA GACGACAACG GAGCAGCACG AGTCCAACAG TCGCGTGGAT 
GGAGGCGGAG GTGTGGAAAA TGCCAGCACC AACGGCCATG GTCGATCCCA CGACAACAAT 
GATGAGAGCC ACACCGCTGT GTCTGACAAG GCTGCAGCCC TCAAAAAGCG GCAATGCGTC 
TTTGCGGAGC TGATGTCCAC GGAGGAGGCA TATGTACAGG ATCTGCATGA GATCGTCAAT 
GGTTACATGA CAGAGATTAA CAACACGAAC AGTGATATAC CCATGCCTGA GGACCTGAAG 
GGTGGTAAAA TGAAGTTGGT CTTCAACAAT ATTAAAGACA TCTACGAGTG GCACAGGGAC 
TTCTTCCTGA GAGCCCTGCG CAACTGCCAA AAATCGCCGG CGGACCTGGG TCCGCTCATC 
AAGCGGTCAG CCACCAAGTT CGCTCTGTAC TACACCTATT GCAGCAACAA ACCGCTGTCC 
GAATACATAG TCAGTGCCCA TTATCAGTAT TTCGATAGCA TACGTCAGAA GCTGGGACAT 
CGTCTGGATC TGAGTAACTT GATCATCAAG CCGGTGCAGC GAATCACTAA ATACGAGCTA 
CTCATCAAGG AGATCATCAA GGCCACCGAG GGAGCCGGGC TTTACAAGGA GGTGCCCATG 
CTGCAGGAGG CCTACCAACA GATGAAGGTC GTCGTCAAGA CGGTCAACGA TATGATGGTG 
GTGCTGCGCA GCTTGCAGGA CTTCGATGGG GAGATAACCG CCCAAGGCAG CCTGTTGATG 
CAGGGACCCA TGAACTGCGT GGTGGATGCG GCACAGAAGC ACCGCGAGCT TCAGGTGTTC 
CTCTTCCAGC AGATCATCAT CTTTGCGGAT ATTGAGAAGA CCAAGAACCA GTATGCCAGT 
CCCATATTCA AATACAGATC GCACATACAG CTCAATCACA TGCAAATGAA GGAACTGGGC 
GACTGCCGCT TTCAAATAAG GTCGACTGAC CCCAAGATCC CAGAGATGAC GATCATCTGC 
CAGGCGGCGT CGCAGGAGAA CTACGCGGGG TGGCGCGATA TGCTAAACAA GATACTGCAG 
CAGCAGAACG ACCTGATCTT CATGCTCTCC AATCCCCTGT CAACCAAAAA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trio ( NM_167850.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728563.1 Drosophila melanogaster trio, transcript variant E (trio), mRNA.

Price & Availability↑

CloneID	OFa19347
Clone ID Related Accession (Same CDS sequence)	NM_167850.2
Accession Version	NM_167850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trio, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_167850.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGTT TCTTCCGCTT ATTTGCCAGC CCCGGCAAAA TGGACGGCAA TCCACTTTCG 
GAAATAGAAG AAATTGTCAA GACGACAACG GAGCAGCACG AGTCCAACAG TCGCGTGGAT 
GGAGGCGGAG GTGTGGAAAA TGCCAGCACC AACGGCCATG GTCGATCCCA CGACAACAAT 
GATGAGAGCC ACACCGCTGT GTCTGACAAG GCTGCAGCCC TCAAAAAGCG GCAATGCGTC 
TTTGCGGAGC TGATGTCCAC GGAGGAGGCA TATGTACAGG ATCTGCATGA GATCGTCAAT 
GGTTACATGA CAGAGATTAA CAACACGAAC AGTGATATAC CCATGCCTGA GGACCTGAAG 
GGTGGTAAAA TGAAGTTGGT CTTCAACAAT ATTAAAGACA TCTACGAGTG GCACAGGGAC 
TTCTTCCTGA GAGCCCTGCG CAACTGCCAA AAATCGCCGG CGGACCTGGG TCCGCTCATC 
AAGCGGTCAG CCACCAAGTT CGCTCTGTAC TACACCTATT GCAGCAACAA ACCGCTGTCC 
GAATACATAG TCAGTGCCCA TTATCAGTAT TTCGATAGCA TACGTCAGAA GCTGGGACAT 
CGTCTGGATC TGAGTAACTT GATCATCAAG CCGGTGCAGC GAATCACTAA ATACGAGCTA 
CTCATCAAGG AGATCATCAA GGCCACCGAG GGAGCCGGGC TTTACAAGGA GGTGCCCATG 
CTGCAGGAGG CCTACCAACA GATGAAGGTC GTCGTCAAGA CGGTCAACGA TATGATGGTG 
GTGCTGCGCA GCTTGCAGGA CTTCGATGGG GAGATAACCG CCCAAGGCAG CCTGTTGATG 
CAGGGACCCA TGAACTGCGT GGTGGATGCG GCACAGAAGC ACCGCGAGCT TCAGGTGTTC 
CTCTTCCAGC AGATCATCAT CTTTGCGGAT ATTGAGAAGA CCAAGAACCA GTATGCCAGT 
CCCATATTCA AATACAGATC GCACATACAG CTCAATCACA TGCAAATGAA GGAACTGGGC 
GACTGCCGCT TTCAAATAAG GTCGACTGAC CCCAAGATCC CAGAGATGAC GATCATCTGC 
CAGGCGGCGT CGCAGGAGAA CTACGCGGGG TGGCGCGATA TGCTAAACAA GATACTGCAG 
CAGCAGAACG ACCTGATCTT CATGCTCTCC AATCCCCTGT CAACCAAAAA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728563.1
CDS	167..1363
Translation	MSCFFRLFASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLMQGPMNCVVDAAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQMKELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPLSTKNK

Target ORF information:

RefSeq Version	NM_167850.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trio, transcript variant E (trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167850.2

ORF Insert Sequence:

ATGAGCTGTT TCTTCCGCTT ATTTGCCAGC CCCGGCAAAA TGGACGGCAA TCCACTTTCG 
GAAATAGAAG AAATTGTCAA GACGACAACG GAGCAGCACG AGTCCAACAG TCGCGTGGAT 
GGAGGCGGAG GTGTGGAAAA TGCCAGCACC AACGGCCATG GTCGATCCCA CGACAACAAT 
GATGAGAGCC ACACCGCTGT GTCTGACAAG GCTGCAGCCC TCAAAAAGCG GCAATGCGTC 
TTTGCGGAGC TGATGTCCAC GGAGGAGGCA TATGTACAGG ATCTGCATGA GATCGTCAAT 
GGTTACATGA CAGAGATTAA CAACACGAAC AGTGATATAC CCATGCCTGA GGACCTGAAG 
GGTGGTAAAA TGAAGTTGGT CTTCAACAAT ATTAAAGACA TCTACGAGTG GCACAGGGAC 
TTCTTCCTGA GAGCCCTGCG CAACTGCCAA AAATCGCCGG CGGACCTGGG TCCGCTCATC 
AAGCGGTCAG CCACCAAGTT CGCTCTGTAC TACACCTATT GCAGCAACAA ACCGCTGTCC 
GAATACATAG TCAGTGCCCA TTATCAGTAT TTCGATAGCA TACGTCAGAA GCTGGGACAT 
CGTCTGGATC TGAGTAACTT GATCATCAAG CCGGTGCAGC GAATCACTAA ATACGAGCTA 
CTCATCAAGG AGATCATCAA GGCCACCGAG GGAGCCGGGC TTTACAAGGA GGTGCCCATG 
CTGCAGGAGG CCTACCAACA GATGAAGGTC GTCGTCAAGA CGGTCAACGA TATGATGGTG 
GTGCTGCGCA GCTTGCAGGA CTTCGATGGG GAGATAACCG CCCAAGGCAG CCTGTTGATG 
CAGGGACCCA TGAACTGCGT GGTGGATGCG GCACAGAAGC ACCGCGAGCT TCAGGTGTTC 
CTCTTCCAGC AGATCATCAT CTTTGCGGAT ATTGAGAAGA CCAAGAACCA GTATGCCAGT 
CCCATATTCA AATACAGATC GCACATACAG CTCAATCACA TGCAAATGAA GGAACTGGGC 
GACTGCCGCT TTCAAATAAG GTCGACTGAC CCCAAGATCC CAGAGATGAC GATCATCTGC 
CAGGCGGCGT CGCAGGAGAA CTACGCGGGG TGGCGCGATA TGCTAAACAA GATACTGCAG 
CAGCAGAACG ACCTGATCTT CATGCTCTCC AATCCCCTGT CAACCAAAAA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trio ( NM_167848.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728561.1 Drosophila melanogaster trio, transcript variant D (trio), mRNA.

Price & Availability↑

CloneID	OFa19345
Clone ID Related Accession (Same CDS sequence)	NM_167848.2
Accession Version	NM_167848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trio, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_167848.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAGTG TTAAGAAGTT TCTCAACGAG CGGATGAACA GTGTGGACTC CGCCGACGGT 
GTTGGCGGGG TCGGCGGCAA GAAGAGCTCG CTGGAGAGGC TCAAGTGCGG CGAGGACACA 
CCGACCAAGG GCTACGTATC CCTGCCCAAT ACACCGCTAC CCACGAGCGC ACCAATCGCC 
GTTGGCGATC CTTCGCCGTC GCCGTCCATT GCAGACTCTG CTCAAAATAC GCCACTCTGC 
ACCCTGCAGG AGGACGACTC CACTTCCCCA CCGCCGGCAG GAGCCGGAAC TCCTGGCAAG 
AATGGCCACA GTCAGCAGGA GCAGTCTCCG TTCACCTTTC CCGCCGTGTC CGCCACGTCA 
TCAAGCAATC CCGCCCCCAA TCCTGCACCA GTTCAGCGGA AAAGTCGCCG CAAGATCTCG 
CTGCCCTGGT TCCGGCAGTC CAGTGTCACG GGTCACGGAG TCTTGGCCCG ACAACATACC 
ATCGATACGC CCAGCTCCTT TCGATTCTTT CGACAGCCCA GCAGCAGCGG ACTCAAGCTG 
GGCAACCAGG AGGCCACCTG GGTGGTAGCG GACTACATTG CCACCTCCGG CAGCAATGAG 
CTGAGCGTGA GCAAGGGGCA GCAGGTGGAG ATTGTCGAGC CGCCCACCGC CGGAGAGCCA 
GACTTCTGCC TGGTGCGCCT GAATCCGCAG CACGACGATG CCGCCGTGCA GGAAGGACTC 
GTTCCTGTCT CAGTGCTCAA ACCGCCGCCG GGCTCACACA AGCACGGTTC CGGAACCGCA 
GCGAATGCAG CCTCATCAGC GGGCTCCCAG AAATCCGACA TGCAGGATCA GAGCAATCGC 
AGCAAGGCGG ATGCACTCTC ATCGTCCACT AAACGTCGCG GTTTCAGCGG ACGCAACTGG 
CTGCCGCTGA TGAATCGCAA GGGAACGGAC AAGCCGCCAA GTAGCAAACC GCTGGTGAAG 
AAGCCCTCGG AGAAAAACCT GAGAACACCC CAGAAGCACG CCGAGGAGCT GGCCGAGCAG 
CAAAACCAGC AGCCAGGTGC CAGTCCCGCC ACCGTGCTGC CATCGTCGCA ATTCCCCAGC 
TCAACTCAGC ATCCAGGAGG AGCAGGAGCC GGCGACTATG AGCCGGACGA GGAGGTCGGC 
CTCGAGCTGC CGCCGCCCAT GAAACCCATC CAAGAGCCGC ACCTGATTGC CAACGGACCG 
CCCGCCTTTG CCAAGGACGC CAAGGAGAGC TCGGGCAACA TGGCCAGCCC CGGCAAAATG 
GACGGCAATC CACTTTCGGA AATAGAAGAA ATTGTCAAGA CGACAACGGA GCAGCACGAG 
TCCAACAGTC GCGTGGATGG AGGCGGAGGT GTGGAAAATG CCAGCACCAA CGGCCATGGT 
CGATCCCACG ACAACAATGA TGAGAGCCAC ACCGCTGTGT CTGACAAGGC TGCAGCCCTC 
AAAAAGCGGC AATGCGTCTT TGCGGAGCTG ATGTCCACGG AGGAGGCATA TGTACAGGAT 
CTGCATGAGA TCGTCAATGG TTACATGACA GAGATTAACA ACACGAACAG TGATATACCC 
ATGCCTGAGG ACCTGAAGGG TGGTAAAATG AAGTTGGTCT TCAACAATAT TAAAGACATC 
TACGAGTGGC ACAGGGACTT CTTCCTGAGA GCCCTGCGCA ACTGCCAAAA ATCGCCGGCG 
GACCTGGGTC CGCTCATCAA GCGGTCAGCC ACCAAGTTCG CTCTGTACTA CACCTATTGC 
AGCAACAAAC CGCTGTCCGA ATACATAGTC AGTGCCCATT ATCAGTATTT CGATAGCATA 
CGTCAGAAGC TGGGACATCG TCTGGATCTG AGTAACTTGA TCATCAAGCC GGTGCAGCGA 
ATCACTAAAT ACGAGCTACT CATCAAGGAG ATCATCAAGG CCACCGAGGG AGCCGGGCTT 
TACAAGGAGG TGCCCATGCT GCAGGAGGCC TACCAACAGA TGAAGGTCGT CGTCAAGACG 
GTCAACGATA TGATGGTGGT GCTGCGCAGC TTGCAGGACT TCGATGGGGA GATAACCGCC 
CAAGGCAGCC TGTTGATGCA GGGACCCATG AACTGCGTGG TGGATGCGGC ACAGAAGCAC 
CGCGAGCTTC AGGTGTTCCT CTTCCAGCAG ATCATCATCT TTGCGGATAT TGAGAAGACC 
AAGAACCAGT ATGCCAGTCC CATATTCAAA TACAGATCGC ACATACAGCT CAATCACATG 
CAAATGAAGG AACTGGGCGA CTGCCGCTTT CAAATAAGGT CGACTGACCC CAAGATCCCA 
GAGATGACGA TCATCTGCCA GGCGGCGTCG CAGGAGAACT ACGCGGGGTG GCGCGATATG 
CTAAACAAGA TACTGCAGCA GCAGAACGAC CTGATCTTCA TGCTCTCCAA TCCCCTGTCA 
ACCAAAAACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728561.1
CDS	387..2801
Translation	MKSVKKFLNERMNSVDSADGVGGVGGKKSSLERLKCGEDTPTKGYVSLPNTPLPTSAPIAVGDPSPSPSIADSAQNTPLCTLQEDDSTSPPPAGAGTPGKNGHSQQEQSPFTFPAVSATSSSNPAPNPAPVQRKSRRKISLPWFRQSSVTGHGVLARQHTIDTPSSFRFFRQPSSSGLKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLMQGPMNCVVDAAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQMKELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPLSTKNK

Target ORF information:

RefSeq Version	NM_167848.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trio, transcript variant D (trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167848.2

ORF Insert Sequence:

ATGAAGAGTG TTAAGAAGTT TCTCAACGAG CGGATGAACA GTGTGGACTC CGCCGACGGT 
GTTGGCGGGG TCGGCGGCAA GAAGAGCTCG CTGGAGAGGC TCAAGTGCGG CGAGGACACA 
CCGACCAAGG GCTACGTATC CCTGCCCAAT ACACCGCTAC CCACGAGCGC ACCAATCGCC 
GTTGGCGATC CTTCGCCGTC GCCGTCCATT GCAGACTCTG CTCAAAATAC GCCACTCTGC 
ACCCTGCAGG AGGACGACTC CACTTCCCCA CCGCCGGCAG GAGCCGGAAC TCCTGGCAAG 
AATGGCCACA GTCAGCAGGA GCAGTCTCCG TTCACCTTTC CCGCCGTGTC CGCCACGTCA 
TCAAGCAATC CCGCCCCCAA TCCTGCACCA GTTCAGCGGA AAAGTCGCCG CAAGATCTCG 
CTGCCCTGGT TCCGGCAGTC CAGTGTCACG GGTCACGGAG TCTTGGCCCG ACAACATACC 
ATCGATACGC CCAGCTCCTT TCGATTCTTT CGACAGCCCA GCAGCAGCGG ACTCAAGCTG 
GGCAACCAGG AGGCCACCTG GGTGGTAGCG GACTACATTG CCACCTCCGG CAGCAATGAG 
CTGAGCGTGA GCAAGGGGCA GCAGGTGGAG ATTGTCGAGC CGCCCACCGC CGGAGAGCCA 
GACTTCTGCC TGGTGCGCCT GAATCCGCAG CACGACGATG CCGCCGTGCA GGAAGGACTC 
GTTCCTGTCT CAGTGCTCAA ACCGCCGCCG GGCTCACACA AGCACGGTTC CGGAACCGCA 
GCGAATGCAG CCTCATCAGC GGGCTCCCAG AAATCCGACA TGCAGGATCA GAGCAATCGC 
AGCAAGGCGG ATGCACTCTC ATCGTCCACT AAACGTCGCG GTTTCAGCGG ACGCAACTGG 
CTGCCGCTGA TGAATCGCAA GGGAACGGAC AAGCCGCCAA GTAGCAAACC GCTGGTGAAG 
AAGCCCTCGG AGAAAAACCT GAGAACACCC CAGAAGCACG CCGAGGAGCT GGCCGAGCAG 
CAAAACCAGC AGCCAGGTGC CAGTCCCGCC ACCGTGCTGC CATCGTCGCA ATTCCCCAGC 
TCAACTCAGC ATCCAGGAGG AGCAGGAGCC GGCGACTATG AGCCGGACGA GGAGGTCGGC 
CTCGAGCTGC CGCCGCCCAT GAAACCCATC CAAGAGCCGC ACCTGATTGC CAACGGACCG 
CCCGCCTTTG CCAAGGACGC CAAGGAGAGC TCGGGCAACA TGGCCAGCCC CGGCAAAATG 
GACGGCAATC CACTTTCGGA AATAGAAGAA ATTGTCAAGA CGACAACGGA GCAGCACGAG 
TCCAACAGTC GCGTGGATGG AGGCGGAGGT GTGGAAAATG CCAGCACCAA CGGCCATGGT 
CGATCCCACG ACAACAATGA TGAGAGCCAC ACCGCTGTGT CTGACAAGGC TGCAGCCCTC 
AAAAAGCGGC AATGCGTCTT TGCGGAGCTG ATGTCCACGG AGGAGGCATA TGTACAGGAT 
CTGCATGAGA TCGTCAATGG TTACATGACA GAGATTAACA ACACGAACAG TGATATACCC 
ATGCCTGAGG ACCTGAAGGG TGGTAAAATG AAGTTGGTCT TCAACAATAT TAAAGACATC 
TACGAGTGGC ACAGGGACTT CTTCCTGAGA GCCCTGCGCA ACTGCCAAAA ATCGCCGGCG 
GACCTGGGTC CGCTCATCAA GCGGTCAGCC ACCAAGTTCG CTCTGTACTA CACCTATTGC 
AGCAACAAAC CGCTGTCCGA ATACATAGTC AGTGCCCATT ATCAGTATTT CGATAGCATA 
CGTCAGAAGC TGGGACATCG TCTGGATCTG AGTAACTTGA TCATCAAGCC GGTGCAGCGA 
ATCACTAAAT ACGAGCTACT CATCAAGGAG ATCATCAAGG CCACCGAGGG AGCCGGGCTT 
TACAAGGAGG TGCCCATGCT GCAGGAGGCC TACCAACAGA TGAAGGTCGT CGTCAAGACG 
GTCAACGATA TGATGGTGGT GCTGCGCAGC TTGCAGGACT TCGATGGGGA GATAACCGCC 
CAAGGCAGCC TGTTGATGCA GGGACCCATG AACTGCGTGG TGGATGCGGC ACAGAAGCAC 
CGCGAGCTTC AGGTGTTCCT CTTCCAGCAG ATCATCATCT TTGCGGATAT TGAGAAGACC 
AAGAACCAGT ATGCCAGTCC CATATTCAAA TACAGATCGC ACATACAGCT CAATCACATG 
CAAATGAAGG AACTGGGCGA CTGCCGCTTT CAAATAAGGT CGACTGACCC CAAGATCCCA 
GAGATGACGA TCATCTGCCA GGCGGCGTCG CAGGAGAACT ACGCGGGGTG GCGCGATATG 
CTAAACAAGA TACTGCAGCA GCAGAACGAC CTGATCTTCA TGCTCTCCAA TCCCCTGTCA 
ACCAAAAACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trio ( NM_143703.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651960.2 Drosophila melanogaster trio, transcript variant A (trio), mRNA.

Price & Availability↑

CloneID	OFa19344
Clone ID Related Accession (Same CDS sequence)	NM_143703.3 , NM_167847.2
Accession Version	NM_143703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trio, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_143703.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGGCC AGCGGGCCAG GGATCTCCTG ACCCTCCTCC AGGAACGGGT CGTCTTCCTC 
ACGGGCGGAC GGGACCGGCG CGGCGGACCG CTCCTCTGCT TCCCGGCCAC GCCCCGTCGC 
GATCGCCTCA AGCCGGAGGA TCTGCGGCGT CTCCTCAGCT ACCTGATCAG CATTCCCAGC 
GACGCCGCCA AGAACCTCGG ATTCACAGTC ATCATTGACA TGCGAGGCAA TGGCAACTGC 
TCGACCAACG TGAAGACGAT ACTGAAGGTG CTGCAGGAGC ACTTCAGCGC GAATATCCAC 
AACGTGGTGA TCATCAAGCC GGACAACTTC TGGCAGAAGC AGCGAGCCTC GATCTCCTCG 
CACAAGTACA AGTTCGAGAC GACGACGGTG TCGATTGAGT CCCTTAACAA AATCGTCGAG 
AGCCATCAGC TGACCGGTGA CTTTGAGGGC CAGCAGCTGT ACGATCACCA GCAGTGGACG 
GACGCACGGT TGGCCATCGA GGACTTCTTC TGGCAAGCCG GGGACATGGC CGATCGAATC 
GACGATCTGC AGGAGGACCT CAACCGCAAC GACTTCGCCG AGGACGTGCC GTTGGCCAGG 
CACGCCATCG ATCACCACAA CGAGATGCGC AAGAAGATCA CCAAGCTGCC CATCGAGGAT 
CTCGACATGC AGGGCAAGAA GCTGCTGGCC AAGATCAATG CGATGGCTCC GCCAGGCGGT 
CAGGCCCCCA CAAACTCTGG CGGACCGGAT ATGGACTCGG GCCCCTCCTC CGCCGGACAG 
CAATCTCAGC CCTCGCAGCA ATCTCGTTCG GTGGGCGGCA ACCCGGACAT GTCTGCCGCC 
GTGAACAAAG CGCTGCGGCA GATCGAACTC ATCCACACCG GTCAGGAGAA ACTTCTGATG 
CTGTGGCAGC ACAAGAAGGT GAAACTAGAC CAGTGCTTCC AGTGGCGCCT GTTCGAACAG 
GACTGCGAGA AGATGTTCGA CTGGATCTTG CACAACCGCG ATGTGTTCCA GATGAGCTAC 
GTGGAGATTG GACACAATTA CTCGGTGGCC AAGTCACTGC AGGACGAGCA CCAGAAGTTC 
GCCGTTGCAT CCATGAATGT ATCCGTGAAT ATCGACAGGA TTTTGGCCGT GGCAGCTCGG 
CTGATCGAAA GTCAGCATTA TGCGGCCCAG CACATTAAAA CGCTGGCTCA ACGTTTGGAC 
CGAACCTGGA AGGACTTTGC CGCGGGCTTG GACGAGAGAA CAGCTGTCCT GCAGCTGAGC 
GTTCTTTTCC ACCACAAGGC AGAACAGTAC TGCAATAGCG TGGCCAGCTG GGCAGCCGCA 
TGTCAGGCCT CTCAGCCTCT GCCCTCGGAT ATCCAGAGCT TGGAAACGGC CATTCGGACT 
CATCAATCCC TGTATGAGGC TATGTGCCAA GCTTACACCG AGGTTCATTC CACTAGCAAG 
AAGCTCCTCT ATCAGTTGGA TCATTTGGTG CAGGTCTGCA ATCAACCTCC GCCGCCTGGT 
GTCCCGGATC ACCGAAAGAA TAGCAACAAT GGTAGTTTTA ACAAGTACGA GAGACAGAAT 
CCAGCAGCGG ATTACAGCGA AGGCGCCAGT CATGTATTGG CTGTGATTCA CCAGATCCTG 
AGCCACCACC GCACCCTGGA GGCCAAGTGG CATCAGGAAA GGCTACGATT GCATCAGACC 
CTAGCTCTTA GACTCTTCCA AGAAGATGTG AAGCAGGTCT TGGATTGGCT GAAAAATCAT 
GGCGAAGTTT TCCTAAGAAA GAATACGGGT ATAGGCAGAA ACTTCCACAA AGCCCGCGTT 
TATCAGACCT CGCACGATAA CTTTGAGAAC GTTGCACAGA ACACCTATAG TAACGCCCAA 
AAACTCCTGG CAGCTGCGGA CGAATTGGCT AGAAGTGGCG AAGCCGATCC CAATGAGATA 
TATTCTGTGG CCAGAGAATT GGAGCTTCAA GTGGGCAGTT TTGCGGAAAG AGTGGAGCAA 
AGAAGACGCC GGCTGGATAT GGCTGTAATA TTCTACACCC ATGAAAAGGA CGTGACCGCC 
TGGATAGACA AACTTCGAAC GGATGTGAGC ACGGATGAAA CGCGTCTTTC GCAGGAGAAC 
CTCGAGGGCA TTGAACGCAT ATTGCAGCAA TACCAGAGGG ATCAGCAGGA GAGCTCCGTC 
AATGTCTGCC TTACAACCAT CTCCCAGGGT GAAGCTCTGC TGCAGGAAAT GCGATCCCTG 
GAGTATGCCG ACAATACTGG TTCCATTGCC GCTCTGGAGG CTACCCTGGA AAAGTTAAAC 
AAACAAAAAG TGGAGTTGGA GGAGCTCTGG TCCGCCCGCA AGTTTCGTGC CGATCTCATA 
CTGCGTTTGC GCTACTTTGA GCGTGATGCC AGATTGCTGT CCGTGCAAAT GGAGATGTGG 
TCCGAGGAGC TCCAGCATGC TGACCTCTCC AGGGACTACC AAAAAGCCGA ACAACTTATT 
CGCATGCACA ACGAGTCGGT GACGGAAATA CAGAACGCCA CTTACGAGGT CGTGCAGCAG 
GGTCAGGACC TGCTGCAGCT CTTTGAGAAT GCTGGCTTCA TTTCCATGGC GGATGCCACG 
CACACGGCTC AGGCTAGGAT CGAGTATCTG CTTGACTTCT TGCGGGAACG GGAGATCGAT 
CTGGAGGAAA TGTCCGAGGC CAAGCGGGCC AAGCTTGAGC AAGCGGTGCA GTTATGTCAA 
TTCCAGAACG ATGCCAACCA GGTTATCTCG TGGATCCGCA ATGGAGAAGC CATGCTGGTG 
GCAAGTTTCG TGACGCCCAA CAGTTTGCAG GAGGCCGAGC AGCTGCGCAA GGGTCATGAG 
CAGTTCCAGA TTGCCATCGA GAAGACGCAC ACATCGGCGG TGCAGGTTAA GTACCGAGCA 
GATGCCCTCA TCAATGCTAA CCATTACGAC CCGCTATCCA TCGATGAAAT TAGCGACGAT 
GTGACGAAGA AGTGGCAGCA GCTGGTGACT TATGCGGAGG AGCGGCACAA GCTGGTGACC 
GCCTCGATAA ACTTCTACAA AACAGCCGAG CAGGTGTGCT CGGTTCTGGA CAGCTTAGAG 
CGGGAGTACA AACGTGAGGA CGACTGGTGT GACGGTGGAG GAGGCAGCGA CAAAGCTCAA 
GCCATTGTCC AGTTGATCTC GAAACATCAG GAGCAGAAGG AGGCTTTCCT AAAGGCCTGT 
ACTTTGGCGC GTCGCACAGC TGAGACTTTT CTAAAATACG CCAACCGATC GCAGCAGTGC 
TATCAGTATA AAGGCAACTG CGAAGGCCAC GTAAAGAGCA AACTGGATAA GCTATTGACT 
CAGGAAAATC AAGTTCTGGA TTACTGGACG CTGCGCAAGA AATCTCTGGA TCAGTGCCAG 
CAGTTCGTTC TCTTTGAGAG AAGCGCCAAA CAGGCGATCG AATGGATTCA CAACACCGGA 
GAAGCGTATC TCTCCTCTAG ATCCAATCTG GTGGGCATAA GCAAGGAGGA AACAGAAGGG 
TTGCTGAAAG AGCATAATGA ATTCCGAAGC ACTGCTAAGG AGACTCGCGA AAGGGTGAAG 
CTTCTGATCC AACTAGCTGA TAGTTTGGTG GAAAAGGGTC ATGCCCATGC GAGCGATATC 
AAGCAATGGG TGGCGTCCGT GGATCAGCGG TACAAGGACT TCAGCAACCG CATGGACAGC 
TACTGCGAGC AGTTGGAGAA GTCTTTGGGC ATGTCCCAGC AGCAGTTACT TCTGGGCTCC 
GATGGAAACA GTTCGATAAG CTCCTCCTCT GGGGATCGTC ATTCGGACCC CACGCTGGAA 
GCCAAGCTAA ACAGCAGCAA TAAGGAGAAC AAGGAGATAA ACGAGGAGAA GCGCAAGTCA 
GCGAGGAGGA AAGAGTTCAT CATGGCTGAA CTGATGCAAA CAGAGAGGGC GTATGTTAAT 
GATCTGGCCA CTTGCATCAA GTGTTTCCTT GAGGAATTCC GAGCGGGAAA AAGTGTACCT 
TCTGCCCTCA TTGGTCAGGA AGATGTGATA TTCGGCAACA TAAAGGAAAT ACACCACTTC 
CATCAGAAGA TTTTCCTGCG CGAGCTGGAG AAATACGAGA CAATGCCTGA GGATGTGGGC 
CATTGCTTTG TGACCTGGGC CTCCAAGTTC GATATGTATG TGCACTACTG CAAGAACAAG 
CCCACATCGA ATAATTTGCT GGTCCAGCAC GGCGGCAGTT TCTTTGAAGA ACTACAACGG 
CGCCTGGAGG TTGATCATCC CCTGCCCGCC TATCTCATCA AACCTGTGCA GAGGATCACC 
AAATACCAGC TGTTACTTAA GGATCTCCTA TCCTGCTGCG AGGAGAGCCA CGGCGAAATC 
AAAGAAGGCC TCGAGGTGAT GCTCAATGTC CCCAAAAAGG CCAACGATGC CATGCATCTT 
TCGCTGCTGG AAAACTGTGA TGTCTCCGTA GACAAACTGG GTGAAGTTGT GCTGCAGGAT 
GCCTTCCAAG CCTGGGATAC CAAGCAGATC ATTCGAAAGG GCAGAGAGCG TCGAGTCTTC 
TTGTTTGAGT TGTATCTCCT GTTCGCTAAG GAGGTAAAGG AGTCCAACGT AGTTAAGTAC 
CAGTTTAAAA GCAAGCTCAT GACCACCGAC ATGGGCATCA CAGAGCACAT CGAAGGCGAT 
GAGACTAAGT TTGCCGTGTG GACAGGTCGT TCTCCCATGC TCTCGGATTG CAGGATCGTG 
CTGAAGGCAA CGAGCTTGGA AACCAAGCAG ATTTGGGTTA AGAAGCTGCG TGAAGTCATG 
CAGGAGACCT GCTTCTCGGG CACCTCGTTG ACCTTGCCCA AGTCTCCAGC TAAGCATTCG 
GGCTCCTCGC AACGATCCTC GCGGGACTTG GATGAACAGC TCACGGAAAA TGATCACGAT 
CGCTGTTCAC TGGCTAGCTT TGGATCGGGT AATACCACAG ATAGTGATAA TAAGCTGGGC 
AACCAGGAGG CCACCTGGGT GGTAGCGGAC TACATTGCCA CCTCCGGCAG CAATGAGCTG 
AGCGTGAGCA AGGGGCAGCA GGTGGAGATT GTCGAGCCGC CCACCGCCGG AGAGCCAGAC 
TTCTGCCTGG TGCGCCTGAA TCCGCAGCAC GACGATGCCG CCGTGCAGGA AGGACTCGTT 
CCTGTCTCAG TGCTCAAACC GCCGCCGGGC TCACACAAGC ACGGTTCCGG AACCGCAGCG 
AATGCAGCCT CATCAGCGGG CTCCCAGAAA TCCGACATGC AGGATCAGAG CAATCGCAGC 
AAGGCGGATG CACTCTCATC GTCCACTAAA CGTCGCGGTT TCAGCGGACG CAACTGGCTG 
CCGCTGATGA ATCGCAAGGG AACGGACAAG CCGCCAAGTA GCAAACCGCT GGTGAAGAAG 
CCCTCGGAGA AAAACCTGAG AACACCCCAG AAGCACGCCG AGGAGCTGGC CGAGCAGCAA 
AACCAGCAGC CAGGTGCCAG TCCCGCCACC GTGCTGCCAT CGTCGCAATT CCCCAGCTCA 
ACTCAGCATC CAGGAGGAGC AGGAGCCGGC GACTATGAGC CGGACGAGGA GGTCGGCCTC 
GAGCTGCCGC CGCCCATGAA ACCCATCCAA GAGCCGCACC TGATTGCCAA CGGACCGCCC 
GCCTTTGCCA AGGACGCCAA GGAGAGCTCG GGCAACATGG CCAGCCCCGG CAAAATGGAC 
GGCAATCCAC TTTCGGAAAT AGAAGAAATT GTCAAGACGA CAACGGAGCA GCACGAGTCC 
AACAGTCGCG TGGATGGAGG CGGAGGTGTG GAAAATGCCA GCACCAACGG CCATGGTCGA 
TCCCACGACA ACAATGATGA GAGCCACACC GCTGTGTCTG ACAAGGCTGC AGCCCTCAAA 
AAGCGGCAAT GCGTCTTTGC GGAGCTGATG TCCACGGAGG AGGCATATGT ACAGGATCTG 
CATGAGATCG TCAATGGTTA CATGACAGAG ATTAACAACA CGAACAGTGA TATACCCATG 
CCTGAGGACC TGAAGGGTGG TAAAATGAAG TTGGTCTTCA ACAATATTAA AGACATCTAC 
GAGTGGCACA GGGACTTCTT CCTGAGAGCC CTGCGCAACT GCCAAAAATC GCCGGCGGAC 
CTGGGTCCGC TCATCAAGCG GTCAGCCACC AAGTTCGCTC TGTACTACAC CTATTGCAGC 
AACAAACCGC TGTCCGAATA CATAGTCAGT GCCCATTATC AGTATTTCGA TAGCATACGT 
CAGAAGCTGG GACATCGTCT GGATCTGAGT AACTTGATCA TCAAGCCGGT GCAGCGAATC 
ACTAAATACG AGCTACTCAT CAAGGAGATC ATCAAGGCCA CCGAGGGAGC CGGGCTTTAC 
AAGGAGGTGC CCATGCTGCA GGAGGCCTAC CAACAGATGA AGGTCGTCGT CAAGACGGTC 
AACGATATGA TGGTGGTGCT GCGCAGCTTG CAGGACTTCG ATGGGGAGAT AACCGCCCAA 
GGCAGCCTGT TGATGCAGGG ACCCATGAAC TGCGTGGTGG ATGCGGCACA GAAGCACCGC 
GAGCTTCAGG TGTTCCTCTT CCAGCAGATC ATCATCTTTG CGGATATTGA GAAGACCAAG 
AACCAGTATG CCAGTCCCAT ATTCAAATAC AGATCGCACA TACAGCTCAA TCACATGCAA 
ATGAAGGAAC TGGGCGACTG CCGCTTTCAA ATAAGGTCGA CTGACCCCAA GATCCCAGAG 
ATGACGATCA TCTGCCAGGC GGCGTCGCAG GAGAACTACG CGGGGTGGCG CGATATGCTA 
AACAAGATAC TGCAGCAGCA GAACGACCTG ATCTTCATGC TCTCCAATCC CCTGTCAACC 
AAAAACAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651960.2
CDS	351..7142
Translation	MDGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNLGFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVSIESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDVPLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQSQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMFDWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQHIKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLETAIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNPAADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLRKNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQVGSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQESSVNVCLTTISQGEALLQEMRSLEYADNTGSIAALEATLEKLNKQKVELEELWSARKFRADLILRLRYFERDARLLSVQMEMWSEELQHADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLEREYKREDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQQCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVKESNVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLMQGPMNCVVDAAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQMKELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPLSTKNK

Target ORF information:

RefSeq Version	NM_143703.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trio, transcript variant A (trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143703.3

ORF Insert Sequence:

ATGGACGGCC AGCGGGCCAG GGATCTCCTG ACCCTCCTCC AGGAACGGGT CGTCTTCCTC 
ACGGGCGGAC GGGACCGGCG CGGCGGACCG CTCCTCTGCT TCCCGGCCAC GCCCCGTCGC 
GATCGCCTCA AGCCGGAGGA TCTGCGGCGT CTCCTCAGCT ACCTGATCAG CATTCCCAGC 
GACGCCGCCA AGAACCTCGG ATTCACAGTC ATCATTGACA TGCGAGGCAA TGGCAACTGC 
TCGACCAACG TGAAGACGAT ACTGAAGGTG CTGCAGGAGC ACTTCAGCGC GAATATCCAC 
AACGTGGTGA TCATCAAGCC GGACAACTTC TGGCAGAAGC AGCGAGCCTC GATCTCCTCG 
CACAAGTACA AGTTCGAGAC GACGACGGTG TCGATTGAGT CCCTTAACAA AATCGTCGAG 
AGCCATCAGC TGACCGGTGA CTTTGAGGGC CAGCAGCTGT ACGATCACCA GCAGTGGACG 
GACGCACGGT TGGCCATCGA GGACTTCTTC TGGCAAGCCG GGGACATGGC CGATCGAATC 
GACGATCTGC AGGAGGACCT CAACCGCAAC GACTTCGCCG AGGACGTGCC GTTGGCCAGG 
CACGCCATCG ATCACCACAA CGAGATGCGC AAGAAGATCA CCAAGCTGCC CATCGAGGAT 
CTCGACATGC AGGGCAAGAA GCTGCTGGCC AAGATCAATG CGATGGCTCC GCCAGGCGGT 
CAGGCCCCCA CAAACTCTGG CGGACCGGAT ATGGACTCGG GCCCCTCCTC CGCCGGACAG 
CAATCTCAGC CCTCGCAGCA ATCTCGTTCG GTGGGCGGCA ACCCGGACAT GTCTGCCGCC 
GTGAACAAAG CGCTGCGGCA GATCGAACTC ATCCACACCG GTCAGGAGAA ACTTCTGATG 
CTGTGGCAGC ACAAGAAGGT GAAACTAGAC CAGTGCTTCC AGTGGCGCCT GTTCGAACAG 
GACTGCGAGA AGATGTTCGA CTGGATCTTG CACAACCGCG ATGTGTTCCA GATGAGCTAC 
GTGGAGATTG GACACAATTA CTCGGTGGCC AAGTCACTGC AGGACGAGCA CCAGAAGTTC 
GCCGTTGCAT CCATGAATGT ATCCGTGAAT ATCGACAGGA TTTTGGCCGT GGCAGCTCGG 
CTGATCGAAA GTCAGCATTA TGCGGCCCAG CACATTAAAA CGCTGGCTCA ACGTTTGGAC 
CGAACCTGGA AGGACTTTGC CGCGGGCTTG GACGAGAGAA CAGCTGTCCT GCAGCTGAGC 
GTTCTTTTCC ACCACAAGGC AGAACAGTAC TGCAATAGCG TGGCCAGCTG GGCAGCCGCA 
TGTCAGGCCT CTCAGCCTCT GCCCTCGGAT ATCCAGAGCT TGGAAACGGC CATTCGGACT 
CATCAATCCC TGTATGAGGC TATGTGCCAA GCTTACACCG AGGTTCATTC CACTAGCAAG 
AAGCTCCTCT ATCAGTTGGA TCATTTGGTG CAGGTCTGCA ATCAACCTCC GCCGCCTGGT 
GTCCCGGATC ACCGAAAGAA TAGCAACAAT GGTAGTTTTA ACAAGTACGA GAGACAGAAT 
CCAGCAGCGG ATTACAGCGA AGGCGCCAGT CATGTATTGG CTGTGATTCA CCAGATCCTG 
AGCCACCACC GCACCCTGGA GGCCAAGTGG CATCAGGAAA GGCTACGATT GCATCAGACC 
CTAGCTCTTA GACTCTTCCA AGAAGATGTG AAGCAGGTCT TGGATTGGCT GAAAAATCAT 
GGCGAAGTTT TCCTAAGAAA GAATACGGGT ATAGGCAGAA ACTTCCACAA AGCCCGCGTT 
TATCAGACCT CGCACGATAA CTTTGAGAAC GTTGCACAGA ACACCTATAG TAACGCCCAA 
AAACTCCTGG CAGCTGCGGA CGAATTGGCT AGAAGTGGCG AAGCCGATCC CAATGAGATA 
TATTCTGTGG CCAGAGAATT GGAGCTTCAA GTGGGCAGTT TTGCGGAAAG AGTGGAGCAA 
AGAAGACGCC GGCTGGATAT GGCTGTAATA TTCTACACCC ATGAAAAGGA CGTGACCGCC 
TGGATAGACA AACTTCGAAC GGATGTGAGC ACGGATGAAA CGCGTCTTTC GCAGGAGAAC 
CTCGAGGGCA TTGAACGCAT ATTGCAGCAA TACCAGAGGG ATCAGCAGGA GAGCTCCGTC 
AATGTCTGCC TTACAACCAT CTCCCAGGGT GAAGCTCTGC TGCAGGAAAT GCGATCCCTG 
GAGTATGCCG ACAATACTGG TTCCATTGCC GCTCTGGAGG CTACCCTGGA AAAGTTAAAC 
AAACAAAAAG TGGAGTTGGA GGAGCTCTGG TCCGCCCGCA AGTTTCGTGC CGATCTCATA 
CTGCGTTTGC GCTACTTTGA GCGTGATGCC AGATTGCTGT CCGTGCAAAT GGAGATGTGG 
TCCGAGGAGC TCCAGCATGC TGACCTCTCC AGGGACTACC AAAAAGCCGA ACAACTTATT 
CGCATGCACA ACGAGTCGGT GACGGAAATA CAGAACGCCA CTTACGAGGT CGTGCAGCAG 
GGTCAGGACC TGCTGCAGCT CTTTGAGAAT GCTGGCTTCA TTTCCATGGC GGATGCCACG 
CACACGGCTC AGGCTAGGAT CGAGTATCTG CTTGACTTCT TGCGGGAACG GGAGATCGAT 
CTGGAGGAAA TGTCCGAGGC CAAGCGGGCC AAGCTTGAGC AAGCGGTGCA GTTATGTCAA 
TTCCAGAACG ATGCCAACCA GGTTATCTCG TGGATCCGCA ATGGAGAAGC CATGCTGGTG 
GCAAGTTTCG TGACGCCCAA CAGTTTGCAG GAGGCCGAGC AGCTGCGCAA GGGTCATGAG 
CAGTTCCAGA TTGCCATCGA GAAGACGCAC ACATCGGCGG TGCAGGTTAA GTACCGAGCA 
GATGCCCTCA TCAATGCTAA CCATTACGAC CCGCTATCCA TCGATGAAAT TAGCGACGAT 
GTGACGAAGA AGTGGCAGCA GCTGGTGACT TATGCGGAGG AGCGGCACAA GCTGGTGACC 
GCCTCGATAA ACTTCTACAA AACAGCCGAG CAGGTGTGCT CGGTTCTGGA CAGCTTAGAG 
CGGGAGTACA AACGTGAGGA CGACTGGTGT GACGGTGGAG GAGGCAGCGA CAAAGCTCAA 
GCCATTGTCC AGTTGATCTC GAAACATCAG GAGCAGAAGG AGGCTTTCCT AAAGGCCTGT 
ACTTTGGCGC GTCGCACAGC TGAGACTTTT CTAAAATACG CCAACCGATC GCAGCAGTGC 
TATCAGTATA AAGGCAACTG CGAAGGCCAC GTAAAGAGCA AACTGGATAA GCTATTGACT 
CAGGAAAATC AAGTTCTGGA TTACTGGACG CTGCGCAAGA AATCTCTGGA TCAGTGCCAG 
CAGTTCGTTC TCTTTGAGAG AAGCGCCAAA CAGGCGATCG AATGGATTCA CAACACCGGA 
GAAGCGTATC TCTCCTCTAG ATCCAATCTG GTGGGCATAA GCAAGGAGGA AACAGAAGGG 
TTGCTGAAAG AGCATAATGA ATTCCGAAGC ACTGCTAAGG AGACTCGCGA AAGGGTGAAG 
CTTCTGATCC AACTAGCTGA TAGTTTGGTG GAAAAGGGTC ATGCCCATGC GAGCGATATC 
AAGCAATGGG TGGCGTCCGT GGATCAGCGG TACAAGGACT TCAGCAACCG CATGGACAGC 
TACTGCGAGC AGTTGGAGAA GTCTTTGGGC ATGTCCCAGC AGCAGTTACT TCTGGGCTCC 
GATGGAAACA GTTCGATAAG CTCCTCCTCT GGGGATCGTC ATTCGGACCC CACGCTGGAA 
GCCAAGCTAA ACAGCAGCAA TAAGGAGAAC AAGGAGATAA ACGAGGAGAA GCGCAAGTCA 
GCGAGGAGGA AAGAGTTCAT CATGGCTGAA CTGATGCAAA CAGAGAGGGC GTATGTTAAT 
GATCTGGCCA CTTGCATCAA GTGTTTCCTT GAGGAATTCC GAGCGGGAAA AAGTGTACCT 
TCTGCCCTCA TTGGTCAGGA AGATGTGATA TTCGGCAACA TAAAGGAAAT ACACCACTTC 
CATCAGAAGA TTTTCCTGCG CGAGCTGGAG AAATACGAGA CAATGCCTGA GGATGTGGGC 
CATTGCTTTG TGACCTGGGC CTCCAAGTTC GATATGTATG TGCACTACTG CAAGAACAAG 
CCCACATCGA ATAATTTGCT GGTCCAGCAC GGCGGCAGTT TCTTTGAAGA ACTACAACGG 
CGCCTGGAGG TTGATCATCC CCTGCCCGCC TATCTCATCA AACCTGTGCA GAGGATCACC 
AAATACCAGC TGTTACTTAA GGATCTCCTA TCCTGCTGCG AGGAGAGCCA CGGCGAAATC 
AAAGAAGGCC TCGAGGTGAT GCTCAATGTC CCCAAAAAGG CCAACGATGC CATGCATCTT 
TCGCTGCTGG AAAACTGTGA TGTCTCCGTA GACAAACTGG GTGAAGTTGT GCTGCAGGAT 
GCCTTCCAAG CCTGGGATAC CAAGCAGATC ATTCGAAAGG GCAGAGAGCG TCGAGTCTTC 
TTGTTTGAGT TGTATCTCCT GTTCGCTAAG GAGGTAAAGG AGTCCAACGT AGTTAAGTAC 
CAGTTTAAAA GCAAGCTCAT GACCACCGAC ATGGGCATCA CAGAGCACAT CGAAGGCGAT 
GAGACTAAGT TTGCCGTGTG GACAGGTCGT TCTCCCATGC TCTCGGATTG CAGGATCGTG 
CTGAAGGCAA CGAGCTTGGA AACCAAGCAG ATTTGGGTTA AGAAGCTGCG TGAAGTCATG 
CAGGAGACCT GCTTCTCGGG CACCTCGTTG ACCTTGCCCA AGTCTCCAGC TAAGCATTCG 
GGCTCCTCGC AACGATCCTC GCGGGACTTG GATGAACAGC TCACGGAAAA TGATCACGAT 
CGCTGTTCAC TGGCTAGCTT TGGATCGGGT AATACCACAG ATAGTGATAA TAAGCTGGGC 
AACCAGGAGG CCACCTGGGT GGTAGCGGAC TACATTGCCA CCTCCGGCAG CAATGAGCTG 
AGCGTGAGCA AGGGGCAGCA GGTGGAGATT GTCGAGCCGC CCACCGCCGG AGAGCCAGAC 
TTCTGCCTGG TGCGCCTGAA TCCGCAGCAC GACGATGCCG CCGTGCAGGA AGGACTCGTT 
CCTGTCTCAG TGCTCAAACC GCCGCCGGGC TCACACAAGC ACGGTTCCGG AACCGCAGCG 
AATGCAGCCT CATCAGCGGG CTCCCAGAAA TCCGACATGC AGGATCAGAG CAATCGCAGC 
AAGGCGGATG CACTCTCATC GTCCACTAAA CGTCGCGGTT TCAGCGGACG CAACTGGCTG 
CCGCTGATGA ATCGCAAGGG AACGGACAAG CCGCCAAGTA GCAAACCGCT GGTGAAGAAG 
CCCTCGGAGA AAAACCTGAG AACACCCCAG AAGCACGCCG AGGAGCTGGC CGAGCAGCAA 
AACCAGCAGC CAGGTGCCAG TCCCGCCACC GTGCTGCCAT CGTCGCAATT CCCCAGCTCA 
ACTCAGCATC CAGGAGGAGC AGGAGCCGGC GACTATGAGC CGGACGAGGA GGTCGGCCTC 
GAGCTGCCGC CGCCCATGAA ACCCATCCAA GAGCCGCACC TGATTGCCAA CGGACCGCCC 
GCCTTTGCCA AGGACGCCAA GGAGAGCTCG GGCAACATGG CCAGCCCCGG CAAAATGGAC 
GGCAATCCAC TTTCGGAAAT AGAAGAAATT GTCAAGACGA CAACGGAGCA GCACGAGTCC 
AACAGTCGCG TGGATGGAGG CGGAGGTGTG GAAAATGCCA GCACCAACGG CCATGGTCGA 
TCCCACGACA ACAATGATGA GAGCCACACC GCTGTGTCTG ACAAGGCTGC AGCCCTCAAA 
AAGCGGCAAT GCGTCTTTGC GGAGCTGATG TCCACGGAGG AGGCATATGT ACAGGATCTG 
CATGAGATCG TCAATGGTTA CATGACAGAG ATTAACAACA CGAACAGTGA TATACCCATG 
CCTGAGGACC TGAAGGGTGG TAAAATGAAG TTGGTCTTCA ACAATATTAA AGACATCTAC 
GAGTGGCACA GGGACTTCTT CCTGAGAGCC CTGCGCAACT GCCAAAAATC GCCGGCGGAC 
CTGGGTCCGC TCATCAAGCG GTCAGCCACC AAGTTCGCTC TGTACTACAC CTATTGCAGC 
AACAAACCGC TGTCCGAATA CATAGTCAGT GCCCATTATC AGTATTTCGA TAGCATACGT 
CAGAAGCTGG GACATCGTCT GGATCTGAGT AACTTGATCA TCAAGCCGGT GCAGCGAATC 
ACTAAATACG AGCTACTCAT CAAGGAGATC ATCAAGGCCA CCGAGGGAGC CGGGCTTTAC 
AAGGAGGTGC CCATGCTGCA GGAGGCCTAC CAACAGATGA AGGTCGTCGT CAAGACGGTC 
AACGATATGA TGGTGGTGCT GCGCAGCTTG CAGGACTTCG ATGGGGAGAT AACCGCCCAA 
GGCAGCCTGT TGATGCAGGG ACCCATGAAC TGCGTGGTGG ATGCGGCACA GAAGCACCGC 
GAGCTTCAGG TGTTCCTCTT CCAGCAGATC ATCATCTTTG CGGATATTGA GAAGACCAAG 
AACCAGTATG CCAGTCCCAT ATTCAAATAC AGATCGCACA TACAGCTCAA TCACATGCAA 
ATGAAGGAAC TGGGCGACTG CCGCTTTCAA ATAAGGTCGA CTGACCCCAA GATCCCAGAG 
ATGACGATCA TCTGCCAGGC GGCGTCGCAG GAGAACTACG CGGGGTGGCG CGATATGCTA 
AACAAGATAC TGCAGCAGCA GAACGACCTG ATCTTCATGC TCTCCAATCC CCTGTCAACC 
AAAAACAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trio ( NM_167847.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728560.1 Drosophila melanogaster trio, transcript variant C (trio), mRNA.

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CloneID	OFa19344
Clone ID Related Accession (Same CDS sequence)	NM_143703.3 , NM_167847.2
Accession Version	NM_167847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trio, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_167847.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGGCC AGCGGGCCAG GGATCTCCTG ACCCTCCTCC AGGAACGGGT CGTCTTCCTC 
ACGGGCGGAC GGGACCGGCG CGGCGGACCG CTCCTCTGCT TCCCGGCCAC GCCCCGTCGC 
GATCGCCTCA AGCCGGAGGA TCTGCGGCGT CTCCTCAGCT ACCTGATCAG CATTCCCAGC 
GACGCCGCCA AGAACCTCGG ATTCACAGTC ATCATTGACA TGCGAGGCAA TGGCAACTGC 
TCGACCAACG TGAAGACGAT ACTGAAGGTG CTGCAGGAGC ACTTCAGCGC GAATATCCAC 
AACGTGGTGA TCATCAAGCC GGACAACTTC TGGCAGAAGC AGCGAGCCTC GATCTCCTCG 
CACAAGTACA AGTTCGAGAC GACGACGGTG TCGATTGAGT CCCTTAACAA AATCGTCGAG 
AGCCATCAGC TGACCGGTGA CTTTGAGGGC CAGCAGCTGT ACGATCACCA GCAGTGGACG 
GACGCACGGT TGGCCATCGA GGACTTCTTC TGGCAAGCCG GGGACATGGC CGATCGAATC 
GACGATCTGC AGGAGGACCT CAACCGCAAC GACTTCGCCG AGGACGTGCC GTTGGCCAGG 
CACGCCATCG ATCACCACAA CGAGATGCGC AAGAAGATCA CCAAGCTGCC CATCGAGGAT 
CTCGACATGC AGGGCAAGAA GCTGCTGGCC AAGATCAATG CGATGGCTCC GCCAGGCGGT 
CAGGCCCCCA CAAACTCTGG CGGACCGGAT ATGGACTCGG GCCCCTCCTC CGCCGGACAG 
CAATCTCAGC CCTCGCAGCA ATCTCGTTCG GTGGGCGGCA ACCCGGACAT GTCTGCCGCC 
GTGAACAAAG CGCTGCGGCA GATCGAACTC ATCCACACCG GTCAGGAGAA ACTTCTGATG 
CTGTGGCAGC ACAAGAAGGT GAAACTAGAC CAGTGCTTCC AGTGGCGCCT GTTCGAACAG 
GACTGCGAGA AGATGTTCGA CTGGATCTTG CACAACCGCG ATGTGTTCCA GATGAGCTAC 
GTGGAGATTG GACACAATTA CTCGGTGGCC AAGTCACTGC AGGACGAGCA CCAGAAGTTC 
GCCGTTGCAT CCATGAATGT ATCCGTGAAT ATCGACAGGA TTTTGGCCGT GGCAGCTCGG 
CTGATCGAAA GTCAGCATTA TGCGGCCCAG CACATTAAAA CGCTGGCTCA ACGTTTGGAC 
CGAACCTGGA AGGACTTTGC CGCGGGCTTG GACGAGAGAA CAGCTGTCCT GCAGCTGAGC 
GTTCTTTTCC ACCACAAGGC AGAACAGTAC TGCAATAGCG TGGCCAGCTG GGCAGCCGCA 
TGTCAGGCCT CTCAGCCTCT GCCCTCGGAT ATCCAGAGCT TGGAAACGGC CATTCGGACT 
CATCAATCCC TGTATGAGGC TATGTGCCAA GCTTACACCG AGGTTCATTC CACTAGCAAG 
AAGCTCCTCT ATCAGTTGGA TCATTTGGTG CAGGTCTGCA ATCAACCTCC GCCGCCTGGT 
GTCCCGGATC ACCGAAAGAA TAGCAACAAT GGTAGTTTTA ACAAGTACGA GAGACAGAAT 
CCAGCAGCGG ATTACAGCGA AGGCGCCAGT CATGTATTGG CTGTGATTCA CCAGATCCTG 
AGCCACCACC GCACCCTGGA GGCCAAGTGG CATCAGGAAA GGCTACGATT GCATCAGACC 
CTAGCTCTTA GACTCTTCCA AGAAGATGTG AAGCAGGTCT TGGATTGGCT GAAAAATCAT 
GGCGAAGTTT TCCTAAGAAA GAATACGGGT ATAGGCAGAA ACTTCCACAA AGCCCGCGTT 
TATCAGACCT CGCACGATAA CTTTGAGAAC GTTGCACAGA ACACCTATAG TAACGCCCAA 
AAACTCCTGG CAGCTGCGGA CGAATTGGCT AGAAGTGGCG AAGCCGATCC CAATGAGATA 
TATTCTGTGG CCAGAGAATT GGAGCTTCAA GTGGGCAGTT TTGCGGAAAG AGTGGAGCAA 
AGAAGACGCC GGCTGGATAT GGCTGTAATA TTCTACACCC ATGAAAAGGA CGTGACCGCC 
TGGATAGACA AACTTCGAAC GGATGTGAGC ACGGATGAAA CGCGTCTTTC GCAGGAGAAC 
CTCGAGGGCA TTGAACGCAT ATTGCAGCAA TACCAGAGGG ATCAGCAGGA GAGCTCCGTC 
AATGTCTGCC TTACAACCAT CTCCCAGGGT GAAGCTCTGC TGCAGGAAAT GCGATCCCTG 
GAGTATGCCG ACAATACTGG TTCCATTGCC GCTCTGGAGG CTACCCTGGA AAAGTTAAAC 
AAACAAAAAG TGGAGTTGGA GGAGCTCTGG TCCGCCCGCA AGTTTCGTGC CGATCTCATA 
CTGCGTTTGC GCTACTTTGA GCGTGATGCC AGATTGCTGT CCGTGCAAAT GGAGATGTGG 
TCCGAGGAGC TCCAGCATGC TGACCTCTCC AGGGACTACC AAAAAGCCGA ACAACTTATT 
CGCATGCACA ACGAGTCGGT GACGGAAATA CAGAACGCCA CTTACGAGGT CGTGCAGCAG 
GGTCAGGACC TGCTGCAGCT CTTTGAGAAT GCTGGCTTCA TTTCCATGGC GGATGCCACG 
CACACGGCTC AGGCTAGGAT CGAGTATCTG CTTGACTTCT TGCGGGAACG GGAGATCGAT 
CTGGAGGAAA TGTCCGAGGC CAAGCGGGCC AAGCTTGAGC AAGCGGTGCA GTTATGTCAA 
TTCCAGAACG ATGCCAACCA GGTTATCTCG TGGATCCGCA ATGGAGAAGC CATGCTGGTG 
GCAAGTTTCG TGACGCCCAA CAGTTTGCAG GAGGCCGAGC AGCTGCGCAA GGGTCATGAG 
CAGTTCCAGA TTGCCATCGA GAAGACGCAC ACATCGGCGG TGCAGGTTAA GTACCGAGCA 
GATGCCCTCA TCAATGCTAA CCATTACGAC CCGCTATCCA TCGATGAAAT TAGCGACGAT 
GTGACGAAGA AGTGGCAGCA GCTGGTGACT TATGCGGAGG AGCGGCACAA GCTGGTGACC 
GCCTCGATAA ACTTCTACAA AACAGCCGAG CAGGTGTGCT CGGTTCTGGA CAGCTTAGAG 
CGGGAGTACA AACGTGAGGA CGACTGGTGT GACGGTGGAG GAGGCAGCGA CAAAGCTCAA 
GCCATTGTCC AGTTGATCTC GAAACATCAG GAGCAGAAGG AGGCTTTCCT AAAGGCCTGT 
ACTTTGGCGC GTCGCACAGC TGAGACTTTT CTAAAATACG CCAACCGATC GCAGCAGTGC 
TATCAGTATA AAGGCAACTG CGAAGGCCAC GTAAAGAGCA AACTGGATAA GCTATTGACT 
CAGGAAAATC AAGTTCTGGA TTACTGGACG CTGCGCAAGA AATCTCTGGA TCAGTGCCAG 
CAGTTCGTTC TCTTTGAGAG AAGCGCCAAA CAGGCGATCG AATGGATTCA CAACACCGGA 
GAAGCGTATC TCTCCTCTAG ATCCAATCTG GTGGGCATAA GCAAGGAGGA AACAGAAGGG 
TTGCTGAAAG AGCATAATGA ATTCCGAAGC ACTGCTAAGG AGACTCGCGA AAGGGTGAAG 
CTTCTGATCC AACTAGCTGA TAGTTTGGTG GAAAAGGGTC ATGCCCATGC GAGCGATATC 
AAGCAATGGG TGGCGTCCGT GGATCAGCGG TACAAGGACT TCAGCAACCG CATGGACAGC 
TACTGCGAGC AGTTGGAGAA GTCTTTGGGC ATGTCCCAGC AGCAGTTACT TCTGGGCTCC 
GATGGAAACA GTTCGATAAG CTCCTCCTCT GGGGATCGTC ATTCGGACCC CACGCTGGAA 
GCCAAGCTAA ACAGCAGCAA TAAGGAGAAC AAGGAGATAA ACGAGGAGAA GCGCAAGTCA 
GCGAGGAGGA AAGAGTTCAT CATGGCTGAA CTGATGCAAA CAGAGAGGGC GTATGTTAAT 
GATCTGGCCA CTTGCATCAA GTGTTTCCTT GAGGAATTCC GAGCGGGAAA AAGTGTACCT 
TCTGCCCTCA TTGGTCAGGA AGATGTGATA TTCGGCAACA TAAAGGAAAT ACACCACTTC 
CATCAGAAGA TTTTCCTGCG CGAGCTGGAG AAATACGAGA CAATGCCTGA GGATGTGGGC 
CATTGCTTTG TGACCTGGGC CTCCAAGTTC GATATGTATG TGCACTACTG CAAGAACAAG 
CCCACATCGA ATAATTTGCT GGTCCAGCAC GGCGGCAGTT TCTTTGAAGA ACTACAACGG 
CGCCTGGAGG TTGATCATCC CCTGCCCGCC TATCTCATCA AACCTGTGCA GAGGATCACC 
AAATACCAGC TGTTACTTAA GGATCTCCTA TCCTGCTGCG AGGAGAGCCA CGGCGAAATC 
AAAGAAGGCC TCGAGGTGAT GCTCAATGTC CCCAAAAAGG CCAACGATGC CATGCATCTT 
TCGCTGCTGG AAAACTGTGA TGTCTCCGTA GACAAACTGG GTGAAGTTGT GCTGCAGGAT 
GCCTTCCAAG CCTGGGATAC CAAGCAGATC ATTCGAAAGG GCAGAGAGCG TCGAGTCTTC 
TTGTTTGAGT TGTATCTCCT GTTCGCTAAG GAGGTAAAGG AGTCCAACGT AGTTAAGTAC 
CAGTTTAAAA GCAAGCTCAT GACCACCGAC ATGGGCATCA CAGAGCACAT CGAAGGCGAT 
GAGACTAAGT TTGCCGTGTG GACAGGTCGT TCTCCCATGC TCTCGGATTG CAGGATCGTG 
CTGAAGGCAA CGAGCTTGGA AACCAAGCAG ATTTGGGTTA AGAAGCTGCG TGAAGTCATG 
CAGGAGACCT GCTTCTCGGG CACCTCGTTG ACCTTGCCCA AGTCTCCAGC TAAGCATTCG 
GGCTCCTCGC AACGATCCTC GCGGGACTTG GATGAACAGC TCACGGAAAA TGATCACGAT 
CGCTGTTCAC TGGCTAGCTT TGGATCGGGT AATACCACAG ATAGTGATAA TAAGCTGGGC 
AACCAGGAGG CCACCTGGGT GGTAGCGGAC TACATTGCCA CCTCCGGCAG CAATGAGCTG 
AGCGTGAGCA AGGGGCAGCA GGTGGAGATT GTCGAGCCGC CCACCGCCGG AGAGCCAGAC 
TTCTGCCTGG TGCGCCTGAA TCCGCAGCAC GACGATGCCG CCGTGCAGGA AGGACTCGTT 
CCTGTCTCAG TGCTCAAACC GCCGCCGGGC TCACACAAGC ACGGTTCCGG AACCGCAGCG 
AATGCAGCCT CATCAGCGGG CTCCCAGAAA TCCGACATGC AGGATCAGAG CAATCGCAGC 
AAGGCGGATG CACTCTCATC GTCCACTAAA CGTCGCGGTT TCAGCGGACG CAACTGGCTG 
CCGCTGATGA ATCGCAAGGG AACGGACAAG CCGCCAAGTA GCAAACCGCT GGTGAAGAAG 
CCCTCGGAGA AAAACCTGAG AACACCCCAG AAGCACGCCG AGGAGCTGGC CGAGCAGCAA 
AACCAGCAGC CAGGTGCCAG TCCCGCCACC GTGCTGCCAT CGTCGCAATT CCCCAGCTCA 
ACTCAGCATC CAGGAGGAGC AGGAGCCGGC GACTATGAGC CGGACGAGGA GGTCGGCCTC 
GAGCTGCCGC CGCCCATGAA ACCCATCCAA GAGCCGCACC TGATTGCCAA CGGACCGCCC 
GCCTTTGCCA AGGACGCCAA GGAGAGCTCG GGCAACATGG CCAGCCCCGG CAAAATGGAC 
GGCAATCCAC TTTCGGAAAT AGAAGAAATT GTCAAGACGA CAACGGAGCA GCACGAGTCC 
AACAGTCGCG TGGATGGAGG CGGAGGTGTG GAAAATGCCA GCACCAACGG CCATGGTCGA 
TCCCACGACA ACAATGATGA GAGCCACACC GCTGTGTCTG ACAAGGCTGC AGCCCTCAAA 
AAGCGGCAAT GCGTCTTTGC GGAGCTGATG TCCACGGAGG AGGCATATGT ACAGGATCTG 
CATGAGATCG TCAATGGTTA CATGACAGAG ATTAACAACA CGAACAGTGA TATACCCATG 
CCTGAGGACC TGAAGGGTGG TAAAATGAAG TTGGTCTTCA ACAATATTAA AGACATCTAC 
GAGTGGCACA GGGACTTCTT CCTGAGAGCC CTGCGCAACT GCCAAAAATC GCCGGCGGAC 
CTGGGTCCGC TCATCAAGCG GTCAGCCACC AAGTTCGCTC TGTACTACAC CTATTGCAGC 
AACAAACCGC TGTCCGAATA CATAGTCAGT GCCCATTATC AGTATTTCGA TAGCATACGT 
CAGAAGCTGG GACATCGTCT GGATCTGAGT AACTTGATCA TCAAGCCGGT GCAGCGAATC 
ACTAAATACG AGCTACTCAT CAAGGAGATC ATCAAGGCCA CCGAGGGAGC CGGGCTTTAC 
AAGGAGGTGC CCATGCTGCA GGAGGCCTAC CAACAGATGA AGGTCGTCGT CAAGACGGTC 
AACGATATGA TGGTGGTGCT GCGCAGCTTG CAGGACTTCG ATGGGGAGAT AACCGCCCAA 
GGCAGCCTGT TGATGCAGGG ACCCATGAAC TGCGTGGTGG ATGCGGCACA GAAGCACCGC 
GAGCTTCAGG TGTTCCTCTT CCAGCAGATC ATCATCTTTG CGGATATTGA GAAGACCAAG 
AACCAGTATG CCAGTCCCAT ATTCAAATAC AGATCGCACA TACAGCTCAA TCACATGCAA 
ATGAAGGAAC TGGGCGACTG CCGCTTTCAA ATAAGGTCGA CTGACCCCAA GATCCCAGAG 
ATGACGATCA TCTGCCAGGC GGCGTCGCAG GAGAACTACG CGGGGTGGCG CGATATGCTA 
AACAAGATAC TGCAGCAGCA GAACGACCTG ATCTTCATGC TCTCCAATCC CCTGTCAACC 
AAAAACAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728560.1
CDS	616..7407
Translation	MDGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNLGFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVSIESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDVPLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQSQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMFDWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQHIKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLETAIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNPAADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLRKNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQVGSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQESSVNVCLTTISQGEALLQEMRSLEYADNTGSIAALEATLEKLNKQKVELEELWSARKFRADLILRLRYFERDARLLSVQMEMWSEELQHADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLEREYKREDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQQCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVKESNVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLMQGPMNCVVDAAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQMKELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPLSTKNK

Target ORF information:

RefSeq Version	NM_167847.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trio, transcript variant C (trio), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167847.2

ORF Insert Sequence:

ATGGACGGCC AGCGGGCCAG GGATCTCCTG ACCCTCCTCC AGGAACGGGT CGTCTTCCTC 
ACGGGCGGAC GGGACCGGCG CGGCGGACCG CTCCTCTGCT TCCCGGCCAC GCCCCGTCGC 
GATCGCCTCA AGCCGGAGGA TCTGCGGCGT CTCCTCAGCT ACCTGATCAG CATTCCCAGC 
GACGCCGCCA AGAACCTCGG ATTCACAGTC ATCATTGACA TGCGAGGCAA TGGCAACTGC 
TCGACCAACG TGAAGACGAT ACTGAAGGTG CTGCAGGAGC ACTTCAGCGC GAATATCCAC 
AACGTGGTGA TCATCAAGCC GGACAACTTC TGGCAGAAGC AGCGAGCCTC GATCTCCTCG 
CACAAGTACA AGTTCGAGAC GACGACGGTG TCGATTGAGT CCCTTAACAA AATCGTCGAG 
AGCCATCAGC TGACCGGTGA CTTTGAGGGC CAGCAGCTGT ACGATCACCA GCAGTGGACG 
GACGCACGGT TGGCCATCGA GGACTTCTTC TGGCAAGCCG GGGACATGGC CGATCGAATC 
GACGATCTGC AGGAGGACCT CAACCGCAAC GACTTCGCCG AGGACGTGCC GTTGGCCAGG 
CACGCCATCG ATCACCACAA CGAGATGCGC AAGAAGATCA CCAAGCTGCC CATCGAGGAT 
CTCGACATGC AGGGCAAGAA GCTGCTGGCC AAGATCAATG CGATGGCTCC GCCAGGCGGT 
CAGGCCCCCA CAAACTCTGG CGGACCGGAT ATGGACTCGG GCCCCTCCTC CGCCGGACAG 
CAATCTCAGC CCTCGCAGCA ATCTCGTTCG GTGGGCGGCA ACCCGGACAT GTCTGCCGCC 
GTGAACAAAG CGCTGCGGCA GATCGAACTC ATCCACACCG GTCAGGAGAA ACTTCTGATG 
CTGTGGCAGC ACAAGAAGGT GAAACTAGAC CAGTGCTTCC AGTGGCGCCT GTTCGAACAG 
GACTGCGAGA AGATGTTCGA CTGGATCTTG CACAACCGCG ATGTGTTCCA GATGAGCTAC 
GTGGAGATTG GACACAATTA CTCGGTGGCC AAGTCACTGC AGGACGAGCA CCAGAAGTTC 
GCCGTTGCAT CCATGAATGT ATCCGTGAAT ATCGACAGGA TTTTGGCCGT GGCAGCTCGG 
CTGATCGAAA GTCAGCATTA TGCGGCCCAG CACATTAAAA CGCTGGCTCA ACGTTTGGAC 
CGAACCTGGA AGGACTTTGC CGCGGGCTTG GACGAGAGAA CAGCTGTCCT GCAGCTGAGC 
GTTCTTTTCC ACCACAAGGC AGAACAGTAC TGCAATAGCG TGGCCAGCTG GGCAGCCGCA 
TGTCAGGCCT CTCAGCCTCT GCCCTCGGAT ATCCAGAGCT TGGAAACGGC CATTCGGACT 
CATCAATCCC TGTATGAGGC TATGTGCCAA GCTTACACCG AGGTTCATTC CACTAGCAAG 
AAGCTCCTCT ATCAGTTGGA TCATTTGGTG CAGGTCTGCA ATCAACCTCC GCCGCCTGGT 
GTCCCGGATC ACCGAAAGAA TAGCAACAAT GGTAGTTTTA ACAAGTACGA GAGACAGAAT 
CCAGCAGCGG ATTACAGCGA AGGCGCCAGT CATGTATTGG CTGTGATTCA CCAGATCCTG 
AGCCACCACC GCACCCTGGA GGCCAAGTGG CATCAGGAAA GGCTACGATT GCATCAGACC 
CTAGCTCTTA GACTCTTCCA AGAAGATGTG AAGCAGGTCT TGGATTGGCT GAAAAATCAT 
GGCGAAGTTT TCCTAAGAAA GAATACGGGT ATAGGCAGAA ACTTCCACAA AGCCCGCGTT 
TATCAGACCT CGCACGATAA CTTTGAGAAC GTTGCACAGA ACACCTATAG TAACGCCCAA 
AAACTCCTGG CAGCTGCGGA CGAATTGGCT AGAAGTGGCG AAGCCGATCC CAATGAGATA 
TATTCTGTGG CCAGAGAATT GGAGCTTCAA GTGGGCAGTT TTGCGGAAAG AGTGGAGCAA 
AGAAGACGCC GGCTGGATAT GGCTGTAATA TTCTACACCC ATGAAAAGGA CGTGACCGCC 
TGGATAGACA AACTTCGAAC GGATGTGAGC ACGGATGAAA CGCGTCTTTC GCAGGAGAAC 
CTCGAGGGCA TTGAACGCAT ATTGCAGCAA TACCAGAGGG ATCAGCAGGA GAGCTCCGTC 
AATGTCTGCC TTACAACCAT CTCCCAGGGT GAAGCTCTGC TGCAGGAAAT GCGATCCCTG 
GAGTATGCCG ACAATACTGG TTCCATTGCC GCTCTGGAGG CTACCCTGGA AAAGTTAAAC 
AAACAAAAAG TGGAGTTGGA GGAGCTCTGG TCCGCCCGCA AGTTTCGTGC CGATCTCATA 
CTGCGTTTGC GCTACTTTGA GCGTGATGCC AGATTGCTGT CCGTGCAAAT GGAGATGTGG 
TCCGAGGAGC TCCAGCATGC TGACCTCTCC AGGGACTACC AAAAAGCCGA ACAACTTATT 
CGCATGCACA ACGAGTCGGT GACGGAAATA CAGAACGCCA CTTACGAGGT CGTGCAGCAG 
GGTCAGGACC TGCTGCAGCT CTTTGAGAAT GCTGGCTTCA TTTCCATGGC GGATGCCACG 
CACACGGCTC AGGCTAGGAT CGAGTATCTG CTTGACTTCT TGCGGGAACG GGAGATCGAT 
CTGGAGGAAA TGTCCGAGGC CAAGCGGGCC AAGCTTGAGC AAGCGGTGCA GTTATGTCAA 
TTCCAGAACG ATGCCAACCA GGTTATCTCG TGGATCCGCA ATGGAGAAGC CATGCTGGTG 
GCAAGTTTCG TGACGCCCAA CAGTTTGCAG GAGGCCGAGC AGCTGCGCAA GGGTCATGAG 
CAGTTCCAGA TTGCCATCGA GAAGACGCAC ACATCGGCGG TGCAGGTTAA GTACCGAGCA 
GATGCCCTCA TCAATGCTAA CCATTACGAC CCGCTATCCA TCGATGAAAT TAGCGACGAT 
GTGACGAAGA AGTGGCAGCA GCTGGTGACT TATGCGGAGG AGCGGCACAA GCTGGTGACC 
GCCTCGATAA ACTTCTACAA AACAGCCGAG CAGGTGTGCT CGGTTCTGGA CAGCTTAGAG 
CGGGAGTACA AACGTGAGGA CGACTGGTGT GACGGTGGAG GAGGCAGCGA CAAAGCTCAA 
GCCATTGTCC AGTTGATCTC GAAACATCAG GAGCAGAAGG AGGCTTTCCT AAAGGCCTGT 
ACTTTGGCGC GTCGCACAGC TGAGACTTTT CTAAAATACG CCAACCGATC GCAGCAGTGC 
TATCAGTATA AAGGCAACTG CGAAGGCCAC GTAAAGAGCA AACTGGATAA GCTATTGACT 
CAGGAAAATC AAGTTCTGGA TTACTGGACG CTGCGCAAGA AATCTCTGGA TCAGTGCCAG 
CAGTTCGTTC TCTTTGAGAG AAGCGCCAAA CAGGCGATCG AATGGATTCA CAACACCGGA 
GAAGCGTATC TCTCCTCTAG ATCCAATCTG GTGGGCATAA GCAAGGAGGA AACAGAAGGG 
TTGCTGAAAG AGCATAATGA ATTCCGAAGC ACTGCTAAGG AGACTCGCGA AAGGGTGAAG 
CTTCTGATCC AACTAGCTGA TAGTTTGGTG GAAAAGGGTC ATGCCCATGC GAGCGATATC 
AAGCAATGGG TGGCGTCCGT GGATCAGCGG TACAAGGACT TCAGCAACCG CATGGACAGC 
TACTGCGAGC AGTTGGAGAA GTCTTTGGGC ATGTCCCAGC AGCAGTTACT TCTGGGCTCC 
GATGGAAACA GTTCGATAAG CTCCTCCTCT GGGGATCGTC ATTCGGACCC CACGCTGGAA 
GCCAAGCTAA ACAGCAGCAA TAAGGAGAAC AAGGAGATAA ACGAGGAGAA GCGCAAGTCA 
GCGAGGAGGA AAGAGTTCAT CATGGCTGAA CTGATGCAAA CAGAGAGGGC GTATGTTAAT 
GATCTGGCCA CTTGCATCAA GTGTTTCCTT GAGGAATTCC GAGCGGGAAA AAGTGTACCT 
TCTGCCCTCA TTGGTCAGGA AGATGTGATA TTCGGCAACA TAAAGGAAAT ACACCACTTC 
CATCAGAAGA TTTTCCTGCG CGAGCTGGAG AAATACGAGA CAATGCCTGA GGATGTGGGC 
CATTGCTTTG TGACCTGGGC CTCCAAGTTC GATATGTATG TGCACTACTG CAAGAACAAG 
CCCACATCGA ATAATTTGCT GGTCCAGCAC GGCGGCAGTT TCTTTGAAGA ACTACAACGG 
CGCCTGGAGG TTGATCATCC CCTGCCCGCC TATCTCATCA AACCTGTGCA GAGGATCACC 
AAATACCAGC TGTTACTTAA GGATCTCCTA TCCTGCTGCG AGGAGAGCCA CGGCGAAATC 
AAAGAAGGCC TCGAGGTGAT GCTCAATGTC CCCAAAAAGG CCAACGATGC CATGCATCTT 
TCGCTGCTGG AAAACTGTGA TGTCTCCGTA GACAAACTGG GTGAAGTTGT GCTGCAGGAT 
GCCTTCCAAG CCTGGGATAC CAAGCAGATC ATTCGAAAGG GCAGAGAGCG TCGAGTCTTC 
TTGTTTGAGT TGTATCTCCT GTTCGCTAAG GAGGTAAAGG AGTCCAACGT AGTTAAGTAC 
CAGTTTAAAA GCAAGCTCAT GACCACCGAC ATGGGCATCA CAGAGCACAT CGAAGGCGAT 
GAGACTAAGT TTGCCGTGTG GACAGGTCGT TCTCCCATGC TCTCGGATTG CAGGATCGTG 
CTGAAGGCAA CGAGCTTGGA AACCAAGCAG ATTTGGGTTA AGAAGCTGCG TGAAGTCATG 
CAGGAGACCT GCTTCTCGGG CACCTCGTTG ACCTTGCCCA AGTCTCCAGC TAAGCATTCG 
GGCTCCTCGC AACGATCCTC GCGGGACTTG GATGAACAGC TCACGGAAAA TGATCACGAT 
CGCTGTTCAC TGGCTAGCTT TGGATCGGGT AATACCACAG ATAGTGATAA TAAGCTGGGC 
AACCAGGAGG CCACCTGGGT GGTAGCGGAC TACATTGCCA CCTCCGGCAG CAATGAGCTG 
AGCGTGAGCA AGGGGCAGCA GGTGGAGATT GTCGAGCCGC CCACCGCCGG AGAGCCAGAC 
TTCTGCCTGG TGCGCCTGAA TCCGCAGCAC GACGATGCCG CCGTGCAGGA AGGACTCGTT 
CCTGTCTCAG TGCTCAAACC GCCGCCGGGC TCACACAAGC ACGGTTCCGG AACCGCAGCG 
AATGCAGCCT CATCAGCGGG CTCCCAGAAA TCCGACATGC AGGATCAGAG CAATCGCAGC 
AAGGCGGATG CACTCTCATC GTCCACTAAA CGTCGCGGTT TCAGCGGACG CAACTGGCTG 
CCGCTGATGA ATCGCAAGGG AACGGACAAG CCGCCAAGTA GCAAACCGCT GGTGAAGAAG 
CCCTCGGAGA AAAACCTGAG AACACCCCAG AAGCACGCCG AGGAGCTGGC CGAGCAGCAA 
AACCAGCAGC CAGGTGCCAG TCCCGCCACC GTGCTGCCAT CGTCGCAATT CCCCAGCTCA 
ACTCAGCATC CAGGAGGAGC AGGAGCCGGC GACTATGAGC CGGACGAGGA GGTCGGCCTC 
GAGCTGCCGC CGCCCATGAA ACCCATCCAA GAGCCGCACC TGATTGCCAA CGGACCGCCC 
GCCTTTGCCA AGGACGCCAA GGAGAGCTCG GGCAACATGG CCAGCCCCGG CAAAATGGAC 
GGCAATCCAC TTTCGGAAAT AGAAGAAATT GTCAAGACGA CAACGGAGCA GCACGAGTCC 
AACAGTCGCG TGGATGGAGG CGGAGGTGTG GAAAATGCCA GCACCAACGG CCATGGTCGA 
TCCCACGACA ACAATGATGA GAGCCACACC GCTGTGTCTG ACAAGGCTGC AGCCCTCAAA 
AAGCGGCAAT GCGTCTTTGC GGAGCTGATG TCCACGGAGG AGGCATATGT ACAGGATCTG 
CATGAGATCG TCAATGGTTA CATGACAGAG ATTAACAACA CGAACAGTGA TATACCCATG 
CCTGAGGACC TGAAGGGTGG TAAAATGAAG TTGGTCTTCA ACAATATTAA AGACATCTAC 
GAGTGGCACA GGGACTTCTT CCTGAGAGCC CTGCGCAACT GCCAAAAATC GCCGGCGGAC 
CTGGGTCCGC TCATCAAGCG GTCAGCCACC AAGTTCGCTC TGTACTACAC CTATTGCAGC 
AACAAACCGC TGTCCGAATA CATAGTCAGT GCCCATTATC AGTATTTCGA TAGCATACGT 
CAGAAGCTGG GACATCGTCT GGATCTGAGT AACTTGATCA TCAAGCCGGT GCAGCGAATC 
ACTAAATACG AGCTACTCAT CAAGGAGATC ATCAAGGCCA CCGAGGGAGC CGGGCTTTAC 
AAGGAGGTGC CCATGCTGCA GGAGGCCTAC CAACAGATGA AGGTCGTCGT CAAGACGGTC 
AACGATATGA TGGTGGTGCT GCGCAGCTTG CAGGACTTCG ATGGGGAGAT AACCGCCCAA 
GGCAGCCTGT TGATGCAGGG ACCCATGAAC TGCGTGGTGG ATGCGGCACA GAAGCACCGC 
GAGCTTCAGG TGTTCCTCTT CCAGCAGATC ATCATCTTTG CGGATATTGA GAAGACCAAG 
AACCAGTATG CCAGTCCCAT ATTCAAATAC AGATCGCACA TACAGCTCAA TCACATGCAA 
ATGAAGGAAC TGGGCGACTG CCGCTTTCAA ATAAGGTCGA CTGACCCCAA GATCCCAGAG 
ATGACGATCA TCTGCCAGGC GGCGTCGCAG GAGAACTACG CGGGGTGGCG CGATATGCTA 
AACAAGATAC TGCAGCAGCA GAACGACCTG ATCTTCATGC TCTCCAATCC CCTGTCAACC 
AAAAACAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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trio cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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