sema6e cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema6e
Entrez Gene ID	556743
Full Name	sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E
Synonyms	bZ1N7.1,si:rp71-1n7.1
General protein information	Preferred Names sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E Names semaphorin-6C semaphorin 6E semaphorin 6db
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009292338.2	XP_009290613.1	semaphorin-6C isoform X3
XM_009292336.2	XP_009290611.1	semaphorin-6C isoform X1
XM_009292336.3	XP_009290611.1	semaphorin-6C isoform X1
XM_021466695.1	XP_021322370.1	semaphorin-6C isoform X1
XM_009292338.3	XP_009290613.1	semaphorin-6C isoform X3
NM_001199365.1	NP_001186294.1	semaphorin-6C precursor
XM_009292337.2	XP_009290612.1	semaphorin-6C isoform X2
XM_009292337.3	XP_009290612.1	semaphorin-6C isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sema6e	NP_001186294.1
Xenopus tropicalis (tropical clawed frog)	LOC100486277	XP_002937315.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Neuronal expression of class 6 semaphorins in zebrafish.
Ebert AM, Lamont RE, Childs SJ, McFarlane S
Gene expression patterns : GEP12(3-4)117-22(2012 Mar-Apr)

Identification and characterization of a novel zebrafish semaphorin.
Taniguchi M, Masuda T, Mikami Y, Kimura M, Yoshida T, Mishina M, Shimizu T
Neuroscience letters488(2)215-20(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we report the cloning and characterization of a novel member of zebrafish semaphorin gene, semaphorin 6E (sema6E).
Title: Identification and characterization of a novel zebrafish semaphorin.

The following sema6e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema6e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33317	NM_001199365.1 Latest version!	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa21588	XM_009292336.3 Latest version!	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa21588	XM_021466695.1 Latest version!	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa21589	XM_009292338.3 Latest version!	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42421	XM_009292337.3 Latest version!	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa21588	XM_009292336.2	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa21589	XM_009292338.2	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42421	XM_009292337.2	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema6e ( NM_001199365.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001186294.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), mRNA.

Price & Availability↑

CloneID	ODa33317
Clone ID Related Accession (Same CDS sequence)	NM_001199365.1
Accession Version	NM_001199365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	semaphorin-6C precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB185147.1. On Dec 8, 2010 this sequence version replaced XM_002667784.1. ##Evidence-Data-START## Transcript exon combination :: AB185147.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAGCCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATTGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTGGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCAAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG AGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTC 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCCAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGC 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAATGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCGTAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCAAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186294.1
CDS	101..3295
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGKTSPTSASTTNQGPAGAPETGTHPEEGRRGGQGSPDLEPGSVEMEGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	NM_001199365.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199365.1

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAGCCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATTGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTGGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCAAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG AGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTC 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCCAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGC 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAATGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCGTAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCAAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6e ( XM_009292336.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290611.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21588
Clone ID Related Accession (Same CDS sequence)	XM_009292336.2 , XM_009292336.3 , XM_021466695.1
Accession Version	XM_009292336.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-6C isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAGTGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCATAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCCAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290611.1
CDS	512..3706
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGKTSPTSASTTNQGPAGAPETGTHPEEGRRGGQGSPDLEPGSVEMEGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	XM_009292336.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292336.2

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAGTGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCATAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCCAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6e ( XM_009292336.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290611.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21588
Clone ID Related Accession (Same CDS sequence)	XM_009292336.2 , XM_009292336.3 , XM_021466695.1
Accession Version	XM_009292336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-6C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAGTGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCATAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCCAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290611.1
CDS	520..3714
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGKTSPTSASTTNQGPAGAPETGTHPEEGRRGGQGSPDLEPGSVEMEGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	XM_009292336.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292336.3

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAGTGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCATAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCCAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6e ( XM_021466695.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322370.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21588
Clone ID Related Accession (Same CDS sequence)	XM_009292336.2 , XM_009292336.3 , XM_021466695.1
Accession Version	XM_021466695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-6C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAGTGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCATAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCCAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322370.1
CDS	175..3369
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGKTSPTSASTTNQGPAGAPETGTHPEEGRRGGQGSPDLEPGSVEMEGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	XM_021466695.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466695.1

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGTACCCA CCCCGAGGAG 
GGGAGGCGGG GAGGGCAGGG GTCTCCTGAC TTGGAGCCCG GCAGTGTGGA GATGGAGGGA 
GTGCGCCGAC CCCCTGAGGT TGATAAGTCC AACCACAGTG TTCACTACAC CCTGCTCATA 
GCGTGTGTCC TGGTGGCGTT CGTCCTGGGA GCTTTCCTCT CCGGATTCCT TGTTTCCTGT 
TACTGCAACC ACAACGTTAA CAAGACGAAA AGGCTGTCGA AAGATCCAGA AGCGCCGATC 
CCACATGCTC TCTCCCTGCG CAGCTTGGCC AAGCTCAATG GCTTGCTGGA GAGCCAAAAC 
AAGGACGAGA AACTAGAGGT GTCCTCACCC AAACTCTACA ACTCATTCTT TGCCAATGGG 
AAAGAGCAAG CACATCGCAG GAATGCCCAC CATGCTGCCA TGACAGAGCT CATTCACCCA 
CATCATCACC ACTCTGCTGA GCTCTCCGGC CTTCCCACAC CAGATTCGAC ACCAGAGCTC 
CCAATCAAGA GCATGAAGGC CTTTAAGAAC CAGTGGGAGA AGAACCAAAA CTGCAATAAC 
GCTAAAGAAC CCAAGTCCCA CAATATGGGA GCATCACGGC CAACAAGTGC AATGCAGCAT 
CAGATGTTCC CTTTCTCTCA TGGGCTGACT AATGGACAGG TACTAGGTGG CTCCGTACAC 
ATGGACGAAC GGAAAGTCCA CAACACGGAG CGTGTGGTAA GCCAACCGTA TCACTGCTAC 
CCGCACAAGA TTGTGGACGT CACCTCACTT GACGAGCTTT TAAAACATAT TCATGAAATC 
AACGTGGCCA CAGGCAAGAG TCCCAGCGTC CTCACCTCGT CCATGTCAGC CAACGCCGGT 
CAGATGGCGT TCGCCAATCG CGTACAGCCA CACATCCCAG AAACAGAGTC CGCACCATAC 
TACAGTTCCT CTACACTTCC TCGGGACAGT CTCACTCGTC GCATGGACAT CCCACCAGAA 
ATTCCTCCCC ATCATCAGTC CACCCTAGAG AGAGTGTCCC GTCACCCAGC TCAACGCCAC 
TCTCTGATAA CCACCCCCAA GATGCCCAGT GGGGGTGTAG TACCTCGGCA GCACAGCTTC 
AACCAACGAA GCTCCCATCA GCCACTCTTG CTCTCCCGAA TGAACTCTAA TGGCAGCAAT 
TCGGGTTGCG ACGTACGGCA CCCACTCATC CCGAACGGAT ACTTAACTCG CCAGCATAGC 
TACAGCGAGC AGCCAGAAGT CCACCGTGGA GCTATTGTTC GTCGGACAGC CTCGCTCAAG 
CCTGACGTCC CACCCAAACC TCTGTTCATC CCTGCCAGCT CACCTGTCAA CCAGGCAGGG 
AAGTTCAACT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6e ( XM_009292338.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290613.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X3, mRNA.

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CloneID	ODa21589
Clone ID Related Accession (Same CDS sequence)	XM_009292338.2 , XM_009292338.3
Accession Version	XM_009292338.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-6C isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAGTGCGCCG ACCCCCTGAG 
GTTGATAAGT CCAACCACAG TGTTCACTAC ACCCTGCTCA TAGCGTGTGT CCTGGTGGCG 
TTCGTCCTGG GAGCTTTCCT CTCCGGATTC CTTGTTTCCT GTTACTGCAA CCACAACGTT 
AACAAGACGA AAAGGCTGTC GAAAGATCCA GAAGCGCCGA TCCCACATGC TCTCTCCCTG 
CGCAGCTTGG CCAAGCTCAA TGGCTTGCTG GAGAGCCAAA ACAAGGACGA GAAACTAGAG 
GTGTCCTCAC CCAAACTCTA CAACTCATTC TTTGCCAATG GGAAAGAGCA AGCACATCGC 
AGGAATGCCC ACCATGCTGC CATGACAGAG CTCATTCACC CACATCATCA CCACTCTGCT 
GAGCTCTCCG GCCTTCCCAC ACCAGATTCG ACACCAGAGC TCCCAATCAA GAGCATGAAG 
GCCTTTAAGA ACCAGTGGGA GAAGAACCAA AACTGCAATA ACGCTAAAGA ACCCAAGTCC 
CACAATATGG GAGCATCACG GCCAACAAGT GCAATGCAGC ATCAGATGTT CCCTTTCTCT 
CATGGGCTGA CTAATGGACA GGTACTAGGT GGCTCCGTAC ACATGGACGA ACGGAAAGTC 
CACAACACGG AGCGTGTGGT AAGCCAACCG TATCACTGCT ACCCGCACAA GATTGTGGAC 
GTCACCTCAC TTGACGAGCT TTTAAAACAT ATTCATGAAA TCAACGTGGC CACAGGCAAG 
AGTCCCAGCG TCCTCACCTC GTCCATGTCA GCCAACGCCG GTCAGATGGC GTTCGCCAAT 
CGCGTACAGC CACACATCCC AGAAACAGAG TCCGCACCAT ACTACAGTTC CTCTACACTT 
CCTCGGGACA GTCTCACTCG TCGCATGGAC ATCCCACCAG AAATTCCTCC CCATCATCAG 
TCCACCCTAG AGAGAGTGTC CCGTCACCCA GCTCAACGCC ACTCTCTGAT AACCACCCCC 
AAGATGCCCA GTGGGGGTGT AGTACCTCGG CAGCACAGCT TCAACCAACG AAGCTCCCAT 
CAGCCACTCT TGCTCTCCCG AATGAACTCT AATGGCAGCA ATTCGGGTTG CGACGTACGG 
CACCCACTCA TCCCGAACGG ATACTTAACT CGCCAGCATA GCTACAGCGA GCAGCCAGAA 
GTCCACCGTG GAGCTATTGT TCGTCGGACA GCCTCGCTCA AGCCTGACGT CCCACCCAAA 
CCTCTGTTCA TCCCTGCCAG CTCACCTGTC AACCAGGCAG GGAAGTTCAA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290613.1
CDS	512..3568
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	XM_009292338.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292338.2

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAGTGCGCCG ACCCCCTGAG 
GTTGATAAGT CCAACCACAG TGTTCACTAC ACCCTGCTCA TAGCGTGTGT CCTGGTGGCG 
TTCGTCCTGG GAGCTTTCCT CTCCGGATTC CTTGTTTCCT GTTACTGCAA CCACAACGTT 
AACAAGACGA AAAGGCTGTC GAAAGATCCA GAAGCGCCGA TCCCACATGC TCTCTCCCTG 
CGCAGCTTGG CCAAGCTCAA TGGCTTGCTG GAGAGCCAAA ACAAGGACGA GAAACTAGAG 
GTGTCCTCAC CCAAACTCTA CAACTCATTC TTTGCCAATG GGAAAGAGCA AGCACATCGC 
AGGAATGCCC ACCATGCTGC CATGACAGAG CTCATTCACC CACATCATCA CCACTCTGCT 
GAGCTCTCCG GCCTTCCCAC ACCAGATTCG ACACCAGAGC TCCCAATCAA GAGCATGAAG 
GCCTTTAAGA ACCAGTGGGA GAAGAACCAA AACTGCAATA ACGCTAAAGA ACCCAAGTCC 
CACAATATGG GAGCATCACG GCCAACAAGT GCAATGCAGC ATCAGATGTT CCCTTTCTCT 
CATGGGCTGA CTAATGGACA GGTACTAGGT GGCTCCGTAC ACATGGACGA ACGGAAAGTC 
CACAACACGG AGCGTGTGGT AAGCCAACCG TATCACTGCT ACCCGCACAA GATTGTGGAC 
GTCACCTCAC TTGACGAGCT TTTAAAACAT ATTCATGAAA TCAACGTGGC CACAGGCAAG 
AGTCCCAGCG TCCTCACCTC GTCCATGTCA GCCAACGCCG GTCAGATGGC GTTCGCCAAT 
CGCGTACAGC CACACATCCC AGAAACAGAG TCCGCACCAT ACTACAGTTC CTCTACACTT 
CCTCGGGACA GTCTCACTCG TCGCATGGAC ATCCCACCAG AAATTCCTCC CCATCATCAG 
TCCACCCTAG AGAGAGTGTC CCGTCACCCA GCTCAACGCC ACTCTCTGAT AACCACCCCC 
AAGATGCCCA GTGGGGGTGT AGTACCTCGG CAGCACAGCT TCAACCAACG AAGCTCCCAT 
CAGCCACTCT TGCTCTCCCG AATGAACTCT AATGGCAGCA ATTCGGGTTG CGACGTACGG 
CACCCACTCA TCCCGAACGG ATACTTAACT CGCCAGCATA GCTACAGCGA GCAGCCAGAA 
GTCCACCGTG GAGCTATTGT TCGTCGGACA GCCTCGCTCA AGCCTGACGT CCCACCCAAA 
CCTCTGTTCA TCCCTGCCAG CTCACCTGTC AACCAGGCAG GGAAGTTCAA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6e ( XM_009292338.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290613.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa21589
Clone ID Related Accession (Same CDS sequence)	XM_009292338.2 , XM_009292338.3
Accession Version	XM_009292338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-6C isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292338.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAGTGCGCCG ACCCCCTGAG 
GTTGATAAGT CCAACCACAG TGTTCACTAC ACCCTGCTCA TAGCGTGTGT CCTGGTGGCG 
TTCGTCCTGG GAGCTTTCCT CTCCGGATTC CTTGTTTCCT GTTACTGCAA CCACAACGTT 
AACAAGACGA AAAGGCTGTC GAAAGATCCA GAAGCGCCGA TCCCACATGC TCTCTCCCTG 
CGCAGCTTGG CCAAGCTCAA TGGCTTGCTG GAGAGCCAAA ACAAGGACGA GAAACTAGAG 
GTGTCCTCAC CCAAACTCTA CAACTCATTC TTTGCCAATG GGAAAGAGCA AGCACATCGC 
AGGAATGCCC ACCATGCTGC CATGACAGAG CTCATTCACC CACATCATCA CCACTCTGCT 
GAGCTCTCCG GCCTTCCCAC ACCAGATTCG ACACCAGAGC TCCCAATCAA GAGCATGAAG 
GCCTTTAAGA ACCAGTGGGA GAAGAACCAA AACTGCAATA ACGCTAAAGA ACCCAAGTCC 
CACAATATGG GAGCATCACG GCCAACAAGT GCAATGCAGC ATCAGATGTT CCCTTTCTCT 
CATGGGCTGA CTAATGGACA GGTACTAGGT GGCTCCGTAC ACATGGACGA ACGGAAAGTC 
CACAACACGG AGCGTGTGGT AAGCCAACCG TATCACTGCT ACCCGCACAA GATTGTGGAC 
GTCACCTCAC TTGACGAGCT TTTAAAACAT ATTCATGAAA TCAACGTGGC CACAGGCAAG 
AGTCCCAGCG TCCTCACCTC GTCCATGTCA GCCAACGCCG GTCAGATGGC GTTCGCCAAT 
CGCGTACAGC CACACATCCC AGAAACAGAG TCCGCACCAT ACTACAGTTC CTCTACACTT 
CCTCGGGACA GTCTCACTCG TCGCATGGAC ATCCCACCAG AAATTCCTCC CCATCATCAG 
TCCACCCTAG AGAGAGTGTC CCGTCACCCA GCTCAACGCC ACTCTCTGAT AACCACCCCC 
AAGATGCCCA GTGGGGGTGT AGTACCTCGG CAGCACAGCT TCAACCAACG AAGCTCCCAT 
CAGCCACTCT TGCTCTCCCG AATGAACTCT AATGGCAGCA ATTCGGGTTG CGACGTACGG 
CACCCACTCA TCCCGAACGG ATACTTAACT CGCCAGCATA GCTACAGCGA GCAGCCAGAA 
GTCCACCGTG GAGCTATTGT TCGTCGGACA GCCTCGCTCA AGCCTGACGT CCCACCCAAA 
CCTCTGTTCA TCCCTGCCAG CTCACCTGTC AACCAGGCAG GGAAGTTCAA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290613.1
CDS	519..3575
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	XM_009292338.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292338.3

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAGTGCGCCG ACCCCCTGAG 
GTTGATAAGT CCAACCACAG TGTTCACTAC ACCCTGCTCA TAGCGTGTGT CCTGGTGGCG 
TTCGTCCTGG GAGCTTTCCT CTCCGGATTC CTTGTTTCCT GTTACTGCAA CCACAACGTT 
AACAAGACGA AAAGGCTGTC GAAAGATCCA GAAGCGCCGA TCCCACATGC TCTCTCCCTG 
CGCAGCTTGG CCAAGCTCAA TGGCTTGCTG GAGAGCCAAA ACAAGGACGA GAAACTAGAG 
GTGTCCTCAC CCAAACTCTA CAACTCATTC TTTGCCAATG GGAAAGAGCA AGCACATCGC 
AGGAATGCCC ACCATGCTGC CATGACAGAG CTCATTCACC CACATCATCA CCACTCTGCT 
GAGCTCTCCG GCCTTCCCAC ACCAGATTCG ACACCAGAGC TCCCAATCAA GAGCATGAAG 
GCCTTTAAGA ACCAGTGGGA GAAGAACCAA AACTGCAATA ACGCTAAAGA ACCCAAGTCC 
CACAATATGG GAGCATCACG GCCAACAAGT GCAATGCAGC ATCAGATGTT CCCTTTCTCT 
CATGGGCTGA CTAATGGACA GGTACTAGGT GGCTCCGTAC ACATGGACGA ACGGAAAGTC 
CACAACACGG AGCGTGTGGT AAGCCAACCG TATCACTGCT ACCCGCACAA GATTGTGGAC 
GTCACCTCAC TTGACGAGCT TTTAAAACAT ATTCATGAAA TCAACGTGGC CACAGGCAAG 
AGTCCCAGCG TCCTCACCTC GTCCATGTCA GCCAACGCCG GTCAGATGGC GTTCGCCAAT 
CGCGTACAGC CACACATCCC AGAAACAGAG TCCGCACCAT ACTACAGTTC CTCTACACTT 
CCTCGGGACA GTCTCACTCG TCGCATGGAC ATCCCACCAG AAATTCCTCC CCATCATCAG 
TCCACCCTAG AGAGAGTGTC CCGTCACCCA GCTCAACGCC ACTCTCTGAT AACCACCCCC 
AAGATGCCCA GTGGGGGTGT AGTACCTCGG CAGCACAGCT TCAACCAACG AAGCTCCCAT 
CAGCCACTCT TGCTCTCCCG AATGAACTCT AATGGCAGCA ATTCGGGTTG CGACGTACGG 
CACCCACTCA TCCCGAACGG ATACTTAACT CGCCAGCATA GCTACAGCGA GCAGCCAGAA 
GTCCACCGTG GAGCTATTGT TCGTCGGACA GCCTCGCTCA AGCCTGACGT CCCACCCAAA 
CCTCTGTTCA TCCCTGCCAG CTCACCTGTC AACCAGGCAG GGAAGTTCAA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6e ( XM_009292337.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290612.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42421
Clone ID Related Accession (Same CDS sequence)	XM_009292337.2 , XM_009292337.3
Accession Version	XM_009292337.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-6C isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGAGTGCG CCGACCCCCT 
GAGGTTGATA AGTCCAACCA CAGTGTTCAC TACACCCTGC TCATAGCGTG TGTCCTGGTG 
GCGTTCGTCC TGGGAGCTTT CCTCTCCGGA TTCCTTGTTT CCTGTTACTG CAACCACAAC 
GTTAACAAGA CGAAAAGGCT GTCGAAAGAT CCAGAAGCGC CGATCCCACA TGCTCTCTCC 
CTGCGCAGCT TGGCCAAGCT CAATGGCTTG CTGGAGAGCC AAAACAAGGA CGAGAAACTA 
GAGGTGTCCT CACCCAAACT CTACAACTCA TTCTTTGCCA ATGGGAAAGA GCAAGCACAT 
CGCAGGAATG CCCACCATGC TGCCATGACA GAGCTCATTC ACCCACATCA TCACCACTCT 
GCTGAGCTCT CCGGCCTTCC CACACCAGAT TCGACACCAG AGCTCCCAAT CAAGAGCATG 
AAGGCCTTTA AGAACCAGTG GGAGAAGAAC CAAAACTGCA ATAACGCTAA AGAACCCAAG 
TCCCACAATA TGGGAGCATC ACGGCCAACA AGTGCAATGC AGCATCAGAT GTTCCCTTTC 
TCTCATGGGC TGACTAATGG ACAGGTACTA GGTGGCTCCG TACACATGGA CGAACGGAAA 
GTCCACAACA CGGAGCGTGT GGTAAGCCAA CCGTATCACT GCTACCCGCA CAAGATTGTG 
GACGTCACCT CACTTGACGA GCTTTTAAAA CATATTCATG AAATCAACGT GGCCACAGGC 
AAGAGTCCCA GCGTCCTCAC CTCGTCCATG TCAGCCAACG CCGGTCAGAT GGCGTTCGCC 
AATCGCGTAC AGCCACACAT CCCAGAAACA GAGTCCGCAC CATACTACAG TTCCTCTACA 
CTTCCTCGGG ACAGTCTCAC TCGTCGCATG GACATCCCAC CAGAAATTCC TCCCCATCAT 
CAGTCCACCC TAGAGAGAGT GTCCCGTCAC CCAGCTCAAC GCCACTCTCT GATAACCACC 
CCCAAGATGC CCAGTGGGGG TGTAGTACCT CGGCAGCACA GCTTCAACCA ACGAAGCTCC 
CATCAGCCAC TCTTGCTCTC CCGAATGAAC TCTAATGGCA GCAATTCGGG TTGCGACGTA 
CGGCACCCAC TCATCCCGAA CGGATACTTA ACTCGCCAGC ATAGCTACAG CGAGCAGCCA 
GAAGTCCACC GTGGAGCTAT TGTTCGTCGG ACAGCCTCGC TCAAGCCTGA CGTCCCACCC 
AAACCTCTGT TCATCCCTGC CAGCTCACCT GTCAACCAGG CAGGGAAGTT CAACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290612.1
CDS	512..3631
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGKTSPTSASTTNQGPAGAPETGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	XM_009292337.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292337.2

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGAGTGCG CCGACCCCCT 
GAGGTTGATA AGTCCAACCA CAGTGTTCAC TACACCCTGC TCATAGCGTG TGTCCTGGTG 
GCGTTCGTCC TGGGAGCTTT CCTCTCCGGA TTCCTTGTTT CCTGTTACTG CAACCACAAC 
GTTAACAAGA CGAAAAGGCT GTCGAAAGAT CCAGAAGCGC CGATCCCACA TGCTCTCTCC 
CTGCGCAGCT TGGCCAAGCT CAATGGCTTG CTGGAGAGCC AAAACAAGGA CGAGAAACTA 
GAGGTGTCCT CACCCAAACT CTACAACTCA TTCTTTGCCA ATGGGAAAGA GCAAGCACAT 
CGCAGGAATG CCCACCATGC TGCCATGACA GAGCTCATTC ACCCACATCA TCACCACTCT 
GCTGAGCTCT CCGGCCTTCC CACACCAGAT TCGACACCAG AGCTCCCAAT CAAGAGCATG 
AAGGCCTTTA AGAACCAGTG GGAGAAGAAC CAAAACTGCA ATAACGCTAA AGAACCCAAG 
TCCCACAATA TGGGAGCATC ACGGCCAACA AGTGCAATGC AGCATCAGAT GTTCCCTTTC 
TCTCATGGGC TGACTAATGG ACAGGTACTA GGTGGCTCCG TACACATGGA CGAACGGAAA 
GTCCACAACA CGGAGCGTGT GGTAAGCCAA CCGTATCACT GCTACCCGCA CAAGATTGTG 
GACGTCACCT CACTTGACGA GCTTTTAAAA CATATTCATG AAATCAACGT GGCCACAGGC 
AAGAGTCCCA GCGTCCTCAC CTCGTCCATG TCAGCCAACG CCGGTCAGAT GGCGTTCGCC 
AATCGCGTAC AGCCACACAT CCCAGAAACA GAGTCCGCAC CATACTACAG TTCCTCTACA 
CTTCCTCGGG ACAGTCTCAC TCGTCGCATG GACATCCCAC CAGAAATTCC TCCCCATCAT 
CAGTCCACCC TAGAGAGAGT GTCCCGTCAC CCAGCTCAAC GCCACTCTCT GATAACCACC 
CCCAAGATGC CCAGTGGGGG TGTAGTACCT CGGCAGCACA GCTTCAACCA ACGAAGCTCC 
CATCAGCCAC TCTTGCTCTC CCGAATGAAC TCTAATGGCA GCAATTCGGG TTGCGACGTA 
CGGCACCCAC TCATCCCGAA CGGATACTTA ACTCGCCAGC ATAGCTACAG CGAGCAGCCA 
GAAGTCCACC GTGGAGCTAT TGTTCGTCGG ACAGCCTCGC TCAAGCCTGA CGTCCCACCC 
AAACCTCTGT TCATCCCTGC CAGCTCACCT GTCAACCAGG CAGGGAAGTT CAACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6e ( XM_009292337.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290612.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X3, mRNA.

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CloneID	ODa42421
Clone ID Related Accession (Same CDS sequence)	XM_009292337.2 , XM_009292337.3
Accession Version	XM_009292337.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-6C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292337.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGAGTGCG CCGACCCCCT 
GAGGTTGATA AGTCCAACCA CAGTGTTCAC TACACCCTGC TCATAGCGTG TGTCCTGGTG 
GCGTTCGTCC TGGGAGCTTT CCTCTCCGGA TTCCTTGTTT CCTGTTACTG CAACCACAAC 
GTTAACAAGA CGAAAAGGCT GTCGAAAGAT CCAGAAGCGC CGATCCCACA TGCTCTCTCC 
CTGCGCAGCT TGGCCAAGCT CAATGGCTTG CTGGAGAGCC AAAACAAGGA CGAGAAACTA 
GAGGTGTCCT CACCCAAACT CTACAACTCA TTCTTTGCCA ATGGGAAAGA GCAAGCACAT 
CGCAGGAATG CCCACCATGC TGCCATGACA GAGCTCATTC ACCCACATCA TCACCACTCT 
GCTGAGCTCT CCGGCCTTCC CACACCAGAT TCGACACCAG AGCTCCCAAT CAAGAGCATG 
AAGGCCTTTA AGAACCAGTG GGAGAAGAAC CAAAACTGCA ATAACGCTAA AGAACCCAAG 
TCCCACAATA TGGGAGCATC ACGGCCAACA AGTGCAATGC AGCATCAGAT GTTCCCTTTC 
TCTCATGGGC TGACTAATGG ACAGGTACTA GGTGGCTCCG TACACATGGA CGAACGGAAA 
GTCCACAACA CGGAGCGTGT GGTAAGCCAA CCGTATCACT GCTACCCGCA CAAGATTGTG 
GACGTCACCT CACTTGACGA GCTTTTAAAA CATATTCATG AAATCAACGT GGCCACAGGC 
AAGAGTCCCA GCGTCCTCAC CTCGTCCATG TCAGCCAACG CCGGTCAGAT GGCGTTCGCC 
AATCGCGTAC AGCCACACAT CCCAGAAACA GAGTCCGCAC CATACTACAG TTCCTCTACA 
CTTCCTCGGG ACAGTCTCAC TCGTCGCATG GACATCCCAC CAGAAATTCC TCCCCATCAT 
CAGTCCACCC TAGAGAGAGT GTCCCGTCAC CCAGCTCAAC GCCACTCTCT GATAACCACC 
CCCAAGATGC CCAGTGGGGG TGTAGTACCT CGGCAGCACA GCTTCAACCA ACGAAGCTCC 
CATCAGCCAC TCTTGCTCTC CCGAATGAAC TCTAATGGCA GCAATTCGGG TTGCGACGTA 
CGGCACCCAC TCATCCCGAA CGGATACTTA ACTCGCCAGC ATAGCTACAG CGAGCAGCCA 
GAAGTCCACC GTGGAGCTAT TGTTCGTCGG ACAGCCTCGC TCAAGCCTGA CGTCCCACCC 
AAACCTCTGT TCATCCCTGC CAGCTCACCT GTCAACCAGG CAGGGAAGTT CAACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290612.1
CDS	519..3638
Translation	MRLVILGSRPLLALTLMLTLTCLKHLCHGATPFPQDLEPIRTVGSEHTYLYPSFQGNMSDNDTARLGLDFQRMLRINHMVYIAARDHVFAINLSASLERIVPQQKLTWKTKDVEKCTVRGKNSDECYNYIKVLVPRNDETLFACGTNAFNPTCRNYKMSSLEQDGEEVIGQARCPFESRQSNVGLFAGGDFYSATMTDFLASDAVIYRSLGESSPVLRTVKYDSKWLREPHFLHAIEYGNYVYFFLSEIAVEYTTLGKVVFSRVARVCKNDNGGSPRVLERYWTSFLKARLNCSVPGDSFFYFDVLQSLTNVMQINHRPAVLGVFTTQANSITGSAVCAFYMDDIEKVFNGKFKEQRNSESAWTPVPDEAVPKPRPGSCAGDGPAAGYKSSTNFPDETLTFIKSYPLMDEAVPSVNNRPCFTRTTSRFKLTQIAVDTAAGPYKNYTVVFLGSENGHVLKVLASMHPNSSYSTQVLEDIDVYNPNKCNVRGEDRRILGLELDKDHHALFVAFTGCVIRVPLSRCSDYGICRKSCLSSRDPYCIWLSAGNCATVAPGFKSGFEQYVESGYPQYPDSCHDVLATTRKQNPAVDSAYGKTSPTSASTTNQGPAGAPETGVRRPPEVDKSNHSVHYTLLIACVLVAFVLGAFLSGFLVSCYCNHNVNKTKRLSKDPEAPIPHALSLRSLAKLNGLLESQNKDEKLEVSSPKLYNSFFANGKEQAHRRNAHHAAMTELIHPHHHHSAELSGLPTPDSTPELPIKSMKAFKNQWEKNQNCNNAKEPKSHNMGASRPTSAMQHQMFPFSHGLTNGQVLGGSVHMDERKVHNTERVVSQPYHCYPHKIVDVTSLDELLKHIHEINVATGKSPSVLTSSMSANAGQMAFANRVQPHIPETESAPYYSSSTLPRDSLTRRMDIPPEIPPHHQSTLERVSRHPAQRHSLITTPKMPSGGVVPRQHSFNQRSSHQPLLLSRMNSNGSNSGCDVRHPLIPNGYLTRQHSYSEQPEVHRGAIVRRTASLKPDVPPKPLFIPASSPVNQAGKFNY

Target ORF information:

RefSeq Version	XM_009292337.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6E (sema6e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292337.3

ORF Insert Sequence:

ATGAGGTTGG TGATTTTGGG GTCGCGGCCC CTTTTGGCCT TGACCCTCAT GCTCACCCTC 
ACCTGCCTAA AGCATCTGTG CCATGGTGCA ACACCCTTTC CTCAGGATCT GGAACCCATC 
AGGACCGTTG GTAGTGAACA CACCTACCTG TACCCCAGTT TCCAGGGGAA CATGTCAGAT 
AACGACACCG CCAGACTGGG CCTGGATTTT CAGAGGATGC TGCGGATTAA CCACATGGTC 
TACATCGCTG CAAGAGATCA CGTTTTTGCC ATTAACCTCA GTGCTTCATT GGAGCGCATC 
GTCCCCCAAC AGAAACTGAC ATGGAAGACA AAAGATGTGG AGAAATGCAC AGTCAGGGGG 
AAAAACAGCG ATGAGTGTTA CAACTACATC AAAGTGCTGG TGCCACGTAA CGATGAAACC 
CTCTTTGCGT GCGGAACCAA TGCGTTCAAC CCCACCTGTC GCAACTACAA GATGTCATCT 
CTAGAGCAGG ACGGAGAAGA GGTGATCGGT CAAGCCCGCT GCCCTTTCGA GTCCCGCCAG 
TCCAACGTTG GCCTGTTTGC CGGCGGTGAC TTCTACTCAG CCACGATGAC TGATTTTCTA 
GCGAGCGATG CAGTGATTTA CCGAAGTTTA GGAGAAAGCA GTCCTGTCCT GCGTACTGTA 
AAATATGACT CCAAGTGGCT GCGAGAACCG CATTTTCTTC ATGCCATCGA GTATGGGAAC 
TACGTGTACT TCTTCCTCAG TGAGATTGCA GTGGAGTATA CCACCCTTGG CAAGGTGGTG 
TTTTCCCGAG TGGCTCGCGT GTGTAAGAAT GATAACGGCG GTTCTCCACG GGTTCTAGAA 
AGATACTGGA CGTCTTTCCT GAAGGCCCGG TTAAACTGCT CAGTTCCTGG TGACTCTTTT 
TTCTACTTTG ATGTTCTTCA GTCATTAACA AACGTCATGC AGATCAACCA CAGGCCTGCA 
GTGTTGGGCG TCTTTACCAC TCAAGCCAAC AGCATCACTG GTTCTGCTGT ATGTGCATTT 
TACATGGATG ACATAGAGAA AGTGTTCAAT GGGAAGTTTA AGGAGCAGCG AAACAGCGAG 
TCCGCCTGGA CACCAGTTCC TGATGAAGCG GTGCCCAAAC CCCGGCCAGG GTCGTGTGCT 
GGTGATGGCC CTGCTGCTGG TTACAAGTCC TCCACAAATT TCCCTGATGA GACTCTTACC 
TTCATTAAGT CTTATCCACT AATGGACGAG GCTGTGCCGT CTGTCAACAA CCGACCCTGC 
TTCACACGAA CCACCAGCAG GTTCAAGCTG ACCCAGATTG CAGTGGACAC GGCAGCTGGC 
CCATATAAGA ACTACACTGT CGTGTTTCTG GGGTCAGAAA ATGGACATGT CCTGAAGGTT 
TTAGCTAGCA TGCATCCAAA CTCCTCGTAC AGCACTCAGG TTTTGGAGGA CATCGATGTT 
TACAACCCTA ACAAATGTAA TGTGCGTGGG GAGGACCGGA GGATTCTGGG ATTGGAGCTG 
GATAAAGACC ATCATGCCCT GTTTGTGGCC TTTACAGGTT GCGTAATCCG TGTTCCACTC 
AGTCGCTGTT CGGATTATGG AATCTGCAGA AAGAGCTGCC TGTCCTCACG AGACCCCTAT 
TGCATCTGGC TGAGCGCAGG GAACTGTGCC ACGGTGGCGC CGGGATTCAA GTCTGGATTC 
GAGCAGTATG TAGAGAGTGG ATATCCTCAG TATCCAGACA GCTGTCACGA TGTCTTGGCA 
ACGACTCGAA AGCAGAACCC TGCAGTGGAC TCTGCATACG GAAAGACCTC ACCCACAAGT 
GCCAGCACAA CCAATCAGGG GCCAGCTGGT GCCCCTGAGA CAGGAGTGCG CCGACCCCCT 
GAGGTTGATA AGTCCAACCA CAGTGTTCAC TACACCCTGC TCATAGCGTG TGTCCTGGTG 
GCGTTCGTCC TGGGAGCTTT CCTCTCCGGA TTCCTTGTTT CCTGTTACTG CAACCACAAC 
GTTAACAAGA CGAAAAGGCT GTCGAAAGAT CCAGAAGCGC CGATCCCACA TGCTCTCTCC 
CTGCGCAGCT TGGCCAAGCT CAATGGCTTG CTGGAGAGCC AAAACAAGGA CGAGAAACTA 
GAGGTGTCCT CACCCAAACT CTACAACTCA TTCTTTGCCA ATGGGAAAGA GCAAGCACAT 
CGCAGGAATG CCCACCATGC TGCCATGACA GAGCTCATTC ACCCACATCA TCACCACTCT 
GCTGAGCTCT CCGGCCTTCC CACACCAGAT TCGACACCAG AGCTCCCAAT CAAGAGCATG 
AAGGCCTTTA AGAACCAGTG GGAGAAGAAC CAAAACTGCA ATAACGCTAA AGAACCCAAG 
TCCCACAATA TGGGAGCATC ACGGCCAACA AGTGCAATGC AGCATCAGAT GTTCCCTTTC 
TCTCATGGGC TGACTAATGG ACAGGTACTA GGTGGCTCCG TACACATGGA CGAACGGAAA 
GTCCACAACA CGGAGCGTGT GGTAAGCCAA CCGTATCACT GCTACCCGCA CAAGATTGTG 
GACGTCACCT CACTTGACGA GCTTTTAAAA CATATTCATG AAATCAACGT GGCCACAGGC 
AAGAGTCCCA GCGTCCTCAC CTCGTCCATG TCAGCCAACG CCGGTCAGAT GGCGTTCGCC 
AATCGCGTAC AGCCACACAT CCCAGAAACA GAGTCCGCAC CATACTACAG TTCCTCTACA 
CTTCCTCGGG ACAGTCTCAC TCGTCGCATG GACATCCCAC CAGAAATTCC TCCCCATCAT 
CAGTCCACCC TAGAGAGAGT GTCCCGTCAC CCAGCTCAAC GCCACTCTCT GATAACCACC 
CCCAAGATGC CCAGTGGGGG TGTAGTACCT CGGCAGCACA GCTTCAACCA ACGAAGCTCC 
CATCAGCCAC TCTTGCTCTC CCGAATGAAC TCTAATGGCA GCAATTCGGG TTGCGACGTA 
CGGCACCCAC TCATCCCGAA CGGATACTTA ACTCGCCAGC ATAGCTACAG CGAGCAGCCA 
GAAGTCCACC GTGGAGCTAT TGTTCGTCGG ACAGCCTCGC TCAAGCCTGA CGTCCCACCC 
AAACCTCTGT TCATCCCTGC CAGCTCACCT GTCAACCAGG CAGGGAAGTT CAACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Neuronal expression of class 6 semaphorins in zebrafish.
Gene expression patterns : GEP12(3-4)117-22(2012 Mar-Apr)
Ebert AM,Lamont RE,Childs SJ,McFarlane S

Identification and characterization of a novel zebrafish semaphorin.
Neuroscience letters488(2)215-20(2011 Jan)
Taniguchi M,Masuda T,Mikami Y,Kimura M,Yoshida T,Mishina M,Shimizu T

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sema6e cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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