shroom2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	shroom2b
Entrez Gene ID	556551
Full Name	shroom family member 2b
General protein information	Preferred Names shroom family member 2b Names protein Shroom2-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021479922.1	XP_021335597.1	protein Shroom2-like isoform X4
XM_005172940.3	XP_005172997.1	protein Shroom2-like isoform X1
XM_679378.9	XP_684470.6	protein Shroom2-like isoform X3
XM_005172940.4	XP_005172997.2	protein Shroom2-like isoform X1
XM_017358378.1	XP_017213867.1	protein Shroom2-like isoform X2
XM_017358378.2	XP_017213867.2	protein Shroom2-like isoform X2
XM_679378.10	XP_684470.7	protein Shroom2-like isoform X3

Pathways from BioSystems

Homologies

	SHROOM2	XP_002806180.1
Rattus norvegicus (Norway rat)	Shroom2	NP_001041358.2
Gallus gallus (chicken)	SHROOM2	XP_416847.3
Homo sapiens (human)	SHROOM2	NP_001640.1
Canis lupus familiaris (dog)	SHROOM2	XP_005641152.1
Pan troglodytes (chimpanzee)	SHROOM2	XP_520924.4
Mus musculus (house mouse)	Shroom2	NP_766029.2
Danio rerio (zebrafish)	LOC556551	XP_005172998.1
Xenopus tropicalis (tropical clawed frog)	shroom2	NP_001072178.1

Gene Ontology

The following shroom2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shroom2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148072	XM_005172940.4 Latest version!	Danio rerio shroom family member 2b (shroom2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148073	XM_017358378.2 Latest version!	Danio rerio shroom family member 2b (shroom2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148074	XM_679378.10 Latest version!	Danio rerio shroom family member 2b (shroom2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa148075	XM_021479922.1 Latest version!	Danio rerio shroom family member 2b (shroom2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa54398	XM_017358378.1 Latest version!	Danio rerio shroom family member 2b (shroom2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa17404	XM_005172940.3 Latest version!	Danio rerio shroom family member 2b (shroom2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa17406	XM_679378.9 Latest version!	Danio rerio shroom family member 2b (shroom2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shroom2b ( XM_005172940.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172997.2 Danio rerio shroom family member 2b (shroom2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148072
Clone ID Related Accession (Same CDS sequence)	XM_005172940.4
Accession Version	XM_005172940.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172940.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGCTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAGTAAGGT GGAAGAGCCA AACACTCCTG GCAGAAAGGT CCCCATACAG 
ATTGTTTGTA CAGAAAATGG TTCAGAGAGA GACATCCGAA ACTATCTGCT GCACTCAGAA 
TCATCTGAAG AGCCTCAAAC TGGCCAGCTA AAAACCCTGG AATCTTCAGA GCAATGTCAT 
GCACCATTCA TCGCATACTC AAACCAGATA GAAGAACCAA TATTAGACCC TTTCAAACCA 
ATGAGGAGCA ACGGTGTGAG CGAACACTCG GAGGACGATC TGAAGCGAGA GGAGCTGGCC 
AGAGATATAA TGGGGAAAGA CAGGAGTCTG GTGGACATTC TGGACCAGAG AAAGATGAAG 
ACCACCATGG ATCTGATGGA GGGAATATTT CCACAGGGCG AGCAGATACT GGAGGGAGCG 
CAGCAGAGGA GGAAAGCGGC GTCCAAACAG ACGTCTCCTA AAAATGCACA GCAGAGGATG 
GAGGAGAGTC TGTCTTCATC AGTCTCGTTG GTCAGCAGTT CATCATATTA CAGCACATCT 
GCACCCAAAG CAGAGCTGCT CATTAAGATG AAGGACATGC AGGAGCAGGA CTCAGAGGAT 
GAGCTCACTA CTGACCTCTC CAGCAAGAAG CAAGAGCTGA TGGACAGTTT GAGCAAGAAG 
CTGCAGGTTC TGAGAGAGGC TCGTGAAAGC CTGCAGGAGG ACGTTCAGGA TAATAACGCT 
CTGGGAGAGG AAGTGGAAGC CACAGTCCAG AGCGTCTGCA GAGCCAATGA GCTGGAGAAG 
TTTCGCATGT TTGTTGGAGA TCTGGATAAA GTGGTCAGTC TGCTGCTGTC TCTGTCTGGA 
CGGCTGGCAC GTGTGGAAAA CGCACTCGAT AACCTGGAGG AGGGAACATC CGCTGACGAG 
AAGCAAACGT TGACGGAGAA GCGCAGGCTG CTGATTGGTC AACATGAAGA CGCCAAAGAG 
CTGAAGGAGA ATCTGGACCG CCGCGAGCGC GTGGTTTATG AGATCCTGAC GGGATACTTC 
AGGGAAGAGC AGCTGGCAGA TTACAGACAC TTCGTTAAAA TGAAATCGGC GCTTATTATA 
GAGCAGCGCA AACTGGAGGA CAAGATAAAA CTGGGAGAAG AGCAGCTGAA GTGTCTGAAG 
GAGAGTCTGC CGCTCGAACA GAGACTCCTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172997.2
CDS	96..4391
Translation	MVDIVTQTMPAESDVHVARNFLTKILRSSMRKNRFKGRNESSCRPHSWHSAKSSESSNPNDSKSEPNPAPDPTWQTKCDPGSPTKEFTSCWGQTNLRQASCQFSSVRNMESVEPSSHAFSKEQPLTSKPNGGADHEVLESAISKPALPGNAFSGTADHGAPRGANPTEGVFYRGDLTVPSDCHRYLQIPIGNGGRVSPSVEEQSSSRLSSTARSNHRPVWHIPENKMSSSEAPPAPPLRSDSFTATRVHEKSLVGLFPEGHTVYPLQRPPCRAVDRQLEDVRHSYNSPPKKDYLEPYLPVEDYPNQLNPTKLFSLSSTDVRQGQNPFASEFQHQRQHSDDSPFSVYNNSTCSSKSQSVGSYYRSLQDLPNNVSNQNKTRTSTVFDPNHDGQAHFRYYCITAQQPYLESSSHCRMVDEQSSEMGKAQGATDRSFVGSQKVIKAKHPQLYLSLPENKRSNGYNKQSISPASAQAVSPISNNLISKHSSVERETQKKKDKPQPSRHIPNHQTEQNSMYTYQHDNPWISKQDLKICPHKTPLLHSLAQESKKMAENSITTASKGGSHESSDASSGKLGRRSDRYATTLRKEIQKKRAQLQKSRSAATLTCSTEVEEDSDIWKSNETSTSSSDGSFTNTYKDHLKEAQAKVLQATSFMRKDLELPGNEVVSAQLPKRPGQVTRIGGRKRFPMDKKIHSFSEPDKINEVGVEDKAGGSFADRYKFFEGSGKAVVQKPIPKQSLLGPTVDHSERKTGEAESIPRVLLKQSKSSHSDLNADEQQRLGTFAEYEATWNLQKKQVERRNTGRYHSAENILDSGLEESNSALCVHERSRSSPSADFYGQKLPLPPRKLPLDSPPEAKDQQEAHISSASEKEHNALNISKSQESITMSILDNSVEPLGYNQKSRSDTKQPAPITSQHLQVPMSEQLLPKNNGLIPQHKELETTCPIMASLEVRIQTFSPEPTQERLRLNTSEPLDGDMHAQVDEHKSERMLSPIPHNSAGSPIPPKQKADLTVTSASRTRSPSPQFFPQRLTDEPPANVYKKDPCSSKVEEPNTPGRKVPIQIVCTENGSERDIRNYLLHSESSEEPQTGQLKTLESSEQCHAPFIAYSNQIEEPILDPFKPMRSNGVSEHSEDDLKREELARDIMGKDRSLVDILDQRKMKTTMDLMEGIFPQGEQILEGAQQRRKAASKQTSPKNAQQRMEESLSSSVSLVSSSSYYSTSAPKAELLIKMKDMQEQDSEDELTTDLSSKKQELMDSLSKKLQVLREARESLQEDVQDNNALGEEVEATVQSVCRANELEKFRMFVGDLDKVVSLLLSLSGRLARVENALDNLEEGTSADEKQTLTEKRRLLIGQHEDAKELKENLDRRERVVYEILTGYFREEQLADYRHFVKMKSALIIEQRKLEDKIKLGEEQLKCLKESLPLEQRLLY

Target ORF information:

RefSeq Version	XM_005172940.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2b (shroom2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172940.4

ORF Insert Sequence:

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGCTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAGTAAGGT GGAAGAGCCA AACACTCCTG GCAGAAAGGT CCCCATACAG 
ATTGTTTGTA CAGAAAATGG TTCAGAGAGA GACATCCGAA ACTATCTGCT GCACTCAGAA 
TCATCTGAAG AGCCTCAAAC TGGCCAGCTA AAAACCCTGG AATCTTCAGA GCAATGTCAT 
GCACCATTCA TCGCATACTC AAACCAGATA GAAGAACCAA TATTAGACCC TTTCAAACCA 
ATGAGGAGCA ACGGTGTGAG CGAACACTCG GAGGACGATC TGAAGCGAGA GGAGCTGGCC 
AGAGATATAA TGGGGAAAGA CAGGAGTCTG GTGGACATTC TGGACCAGAG AAAGATGAAG 
ACCACCATGG ATCTGATGGA GGGAATATTT CCACAGGGCG AGCAGATACT GGAGGGAGCG 
CAGCAGAGGA GGAAAGCGGC GTCCAAACAG ACGTCTCCTA AAAATGCACA GCAGAGGATG 
GAGGAGAGTC TGTCTTCATC AGTCTCGTTG GTCAGCAGTT CATCATATTA CAGCACATCT 
GCACCCAAAG CAGAGCTGCT CATTAAGATG AAGGACATGC AGGAGCAGGA CTCAGAGGAT 
GAGCTCACTA CTGACCTCTC CAGCAAGAAG CAAGAGCTGA TGGACAGTTT GAGCAAGAAG 
CTGCAGGTTC TGAGAGAGGC TCGTGAAAGC CTGCAGGAGG ACGTTCAGGA TAATAACGCT 
CTGGGAGAGG AAGTGGAAGC CACAGTCCAG AGCGTCTGCA GAGCCAATGA GCTGGAGAAG 
TTTCGCATGT TTGTTGGAGA TCTGGATAAA GTGGTCAGTC TGCTGCTGTC TCTGTCTGGA 
CGGCTGGCAC GTGTGGAAAA CGCACTCGAT AACCTGGAGG AGGGAACATC CGCTGACGAG 
AAGCAAACGT TGACGGAGAA GCGCAGGCTG CTGATTGGTC AACATGAAGA CGCCAAAGAG 
CTGAAGGAGA ATCTGGACCG CCGCGAGCGC GTGGTTTATG AGATCCTGAC GGGATACTTC 
AGGGAAGAGC AGCTGGCAGA TTACAGACAC TTCGTTAAAA TGAAATCGGC GCTTATTATA 
GAGCAGCGCA AACTGGAGGA CAAGATAAAA CTGGGAGAAG AGCAGCTGAA GTGTCTGAAG 
GAGAGTCTGC CGCTCGAACA GAGACTCCTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2b ( XM_017358378.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213867.2 Danio rerio shroom family member 2b (shroom2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa148073
Clone ID Related Accession (Same CDS sequence)	XM_017358378.2
Accession Version	XM_017358378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGCTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAAGGTGGA AGAGCCAAAC ACTCCTGGCA GAAAGGTCCC CATACAGATT 
GTTTGTACAG AAAATGGTTC AGAGAGAGAC ATCCGAAACT ATCTGCTGCA CTCAGAATCA 
TCTGAAGAGC CTCAAACTGG CCAGCTAAAA ACCCTGGAAT CTTCAGAGCA ATGTCATGCA 
CCATTCATCG CATACTCAAA CCAGATAGAA GAACCAATAT TAGACCCTTT CAAACCAATG 
AGGAGCAACG GTGTGAGCGA ACACTCGGAG GACGATCTGA AGCGAGAGGA GCTGGCCAGA 
GATATAATGG GGAAAGACAG GAGTCTGGTG GACATTCTGG ACCAGAGAAA GATGAAGACC 
ACCATGGATC TGATGGAGGG AATATTTCCA CAGGGCGAGC AGATACTGGA GGGAGCGCAG 
CAGAGGAGGA AAGCGGCGTC CAAACAGACG TCTCCTAAAA ATGCACAGCA GAGGATGGAG 
GAGAGTCTGT CTTCATCAGT CTCGTTGGTC AGCAGTTCAT CATATTACAG CACATCTGCA 
CCCAAAGCAG AGCTGCTCAT TAAGATGAAG GACATGCAGG AGCAGGACTC AGAGGATGAG 
CTCACTACTG ACCTCTCCAG CAAGAAGCAA GAGCTGATGG ACAGTTTGAG CAAGAAGCTG 
CAGGTTCTGA GAGAGGCTCG TGAAAGCCTG CAGGAGGACG TTCAGGATAA TAACGCTCTG 
GGAGAGGAAG TGGAAGCCAC AGTCCAGAGC GTCTGCAGAG CCAATGAGCT GGAGAAGTTT 
CGCATGTTTG TTGGAGATCT GGATAAAGTG GTCAGTCTGC TGCTGTCTCT GTCTGGACGG 
CTGGCACGTG TGGAAAACGC ACTCGATAAC CTGGAGGAGG GAACATCCGC TGACGAGAAG 
CAAACGTTGA CGGAGAAGCG CAGGCTGCTG ATTGGTCAAC ATGAAGACGC CAAAGAGCTG 
AAGGAGAATC TGGACCGCCG CGAGCGCGTG GTTTATGAGA TCCTGACGGG ATACTTCAGG 
GAAGAGCAGC TGGCAGATTA CAGACACTTC GTTAAAATGA AATCGGCGCT TATTATAGAG 
CAGCGCAAAC TGGAGGACAA GATAAAACTG GGAGAAGAGC AGCTGAAGTG TCTGAAGGAG 
AGTCTGCCGC TCGAACAGAG ACTCCTGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213867.2
CDS	96..4388
Translation	MVDIVTQTMPAESDVHVARNFLTKILRSSMRKNRFKGRNESSCRPHSWHSAKSSESSNPNDSKSEPNPAPDPTWQTKCDPGSPTKEFTSCWGQTNLRQASCQFSSVRNMESVEPSSHAFSKEQPLTSKPNGGADHEVLESAISKPALPGNAFSGTADHGAPRGANPTEGVFYRGDLTVPSDCHRYLQIPIGNGGRVSPSVEEQSSSRLSSTARSNHRPVWHIPENKMSSSEAPPAPPLRSDSFTATRVHEKSLVGLFPEGHTVYPLQRPPCRAVDRQLEDVRHSYNSPPKKDYLEPYLPVEDYPNQLNPTKLFSLSSTDVRQGQNPFASEFQHQRQHSDDSPFSVYNNSTCSSKSQSVGSYYRSLQDLPNNVSNQNKTRTSTVFDPNHDGQAHFRYYCITAQQPYLESSSHCRMVDEQSSEMGKAQGATDRSFVGSQKVIKAKHPQLYLSLPENKRSNGYNKQSISPASAQAVSPISNNLISKHSSVERETQKKKDKPQPSRHIPNHQTEQNSMYTYQHDNPWISKQDLKICPHKTPLLHSLAQESKKMAENSITTASKGGSHESSDASSGKLGRRSDRYATTLRKEIQKKRAQLQKSRSAATLTCSTEVEEDSDIWKSNETSTSSSDGSFTNTYKDHLKEAQAKVLQATSFMRKDLELPGNEVVSAQLPKRPGQVTRIGGRKRFPMDKKIHSFSEPDKINEVGVEDKAGGSFADRYKFFEGSGKAVVQKPIPKQSLLGPTVDHSERKTGEAESIPRVLLKQSKSSHSDLNADEQQRLGTFAEYEATWNLQKKQVERRNTGRYHSAENILDSGLEESNSALCVHERSRSSPSADFYGQKLPLPPRKLPLDSPPEAKDQQEAHISSASEKEHNALNISKSQESITMSILDNSVEPLGYNQKSRSDTKQPAPITSQHLQVPMSEQLLPKNNGLIPQHKELETTCPIMASLEVRIQTFSPEPTQERLRLNTSEPLDGDMHAQVDEHKSERMLSPIPHNSAGSPIPPKQKADLTVTSASRTRSPSPQFFPQRLTDEPPANVYKKDPCSKVEEPNTPGRKVPIQIVCTENGSERDIRNYLLHSESSEEPQTGQLKTLESSEQCHAPFIAYSNQIEEPILDPFKPMRSNGVSEHSEDDLKREELARDIMGKDRSLVDILDQRKMKTTMDLMEGIFPQGEQILEGAQQRRKAASKQTSPKNAQQRMEESLSSSVSLVSSSSYYSTSAPKAELLIKMKDMQEQDSEDELTTDLSSKKQELMDSLSKKLQVLREARESLQEDVQDNNALGEEVEATVQSVCRANELEKFRMFVGDLDKVVSLLLSLSGRLARVENALDNLEEGTSADEKQTLTEKRRLLIGQHEDAKELKENLDRRERVVYEILTGYFREEQLADYRHFVKMKSALIIEQRKLEDKIKLGEEQLKCLKESLPLEQRLLY

Target ORF information:

RefSeq Version	XM_017358378.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2b (shroom2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358378.2

ORF Insert Sequence:

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGCTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAAGGTGGA AGAGCCAAAC ACTCCTGGCA GAAAGGTCCC CATACAGATT 
GTTTGTACAG AAAATGGTTC AGAGAGAGAC ATCCGAAACT ATCTGCTGCA CTCAGAATCA 
TCTGAAGAGC CTCAAACTGG CCAGCTAAAA ACCCTGGAAT CTTCAGAGCA ATGTCATGCA 
CCATTCATCG CATACTCAAA CCAGATAGAA GAACCAATAT TAGACCCTTT CAAACCAATG 
AGGAGCAACG GTGTGAGCGA ACACTCGGAG GACGATCTGA AGCGAGAGGA GCTGGCCAGA 
GATATAATGG GGAAAGACAG GAGTCTGGTG GACATTCTGG ACCAGAGAAA GATGAAGACC 
ACCATGGATC TGATGGAGGG AATATTTCCA CAGGGCGAGC AGATACTGGA GGGAGCGCAG 
CAGAGGAGGA AAGCGGCGTC CAAACAGACG TCTCCTAAAA ATGCACAGCA GAGGATGGAG 
GAGAGTCTGT CTTCATCAGT CTCGTTGGTC AGCAGTTCAT CATATTACAG CACATCTGCA 
CCCAAAGCAG AGCTGCTCAT TAAGATGAAG GACATGCAGG AGCAGGACTC AGAGGATGAG 
CTCACTACTG ACCTCTCCAG CAAGAAGCAA GAGCTGATGG ACAGTTTGAG CAAGAAGCTG 
CAGGTTCTGA GAGAGGCTCG TGAAAGCCTG CAGGAGGACG TTCAGGATAA TAACGCTCTG 
GGAGAGGAAG TGGAAGCCAC AGTCCAGAGC GTCTGCAGAG CCAATGAGCT GGAGAAGTTT 
CGCATGTTTG TTGGAGATCT GGATAAAGTG GTCAGTCTGC TGCTGTCTCT GTCTGGACGG 
CTGGCACGTG TGGAAAACGC ACTCGATAAC CTGGAGGAGG GAACATCCGC TGACGAGAAG 
CAAACGTTGA CGGAGAAGCG CAGGCTGCTG ATTGGTCAAC ATGAAGACGC CAAAGAGCTG 
AAGGAGAATC TGGACCGCCG CGAGCGCGTG GTTTATGAGA TCCTGACGGG ATACTTCAGG 
GAAGAGCAGC TGGCAGATTA CAGACACTTC GTTAAAATGA AATCGGCGCT TATTATAGAG 
CAGCGCAAAC TGGAGGACAA GATAAAACTG GGAGAAGAGC AGCTGAAGTG TCTGAAGGAG 
AGTCTGCCGC TCGAACAGAG ACTCCTGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2b ( XM_679378.10 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_684470.7 Danio rerio shroom family member 2b (shroom2b), transcript variant X3, mRNA.

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CloneID	ODa148074
Clone ID Related Accession (Same CDS sequence)	XM_679378.10
Accession Version	XM_679378.10 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679378.9. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAGGAATG AATCGTCATG TAGACCCCAT 
TCATGGCACT CAGCTAAATC CTCGGAAAGC TCCAACCCAA ACGACTCCAA ATCTGAACCC 
AACCCAGCAC CTGACCCCAC TTGGCAGACA AAATGTGATC CTGGGTCACC CACAAAAGAA 
TTCACCAGCT GTTGGGGACA GACCAATCTG CGACAAGCAT CCTGCCAGTT CAGCTCGGTC 
AGAAATATGG AAAGCGTAGA GCCCTCATCT CATGCTTTCT CAAAAGAACA ACCTTTAACA 
AGCAAACCCA ATGGAGGAGC AGATCACGAG GTGCTTGAAA GTGCAATCAG CAAACCTGCT 
TTGCCAGGCA ATGCATTTTC AGGCACAGCT GATCATGGTG CACCTCGGGG TGCCAATCCC 
ACAGAAGGGG TGTTTTACAG AGGCGATCTA ACAGTCCCGA GTGACTGCCA CAGATACCTG 
CAGATCCCCA TAGGCAATGG GGGACGAGTG AGTCCCAGTG TTGAGGAGCA ATCTAGCTCC 
AGACTCTCCT CTACTGCGAG ATCAAACCAT AGACCTGTGT GGCATATTCC AGAGAATAAG 
ATGTCCAGTT CTGAAGCTCC TCCTGCTCCT CCTTTGCGGA GTGATAGTTT CACTGCCACC 
AGAGTTCATG AGAAGAGCTT AGTGGGATTG TTCCCTGAGG GACACACTGT ATATCCACTA 
CAGAGACCTC CCTGTAGAGC TGTGGACAGA CAATTAGAGG ACGTGAGACA CAGCTACAAC 
TCGCCTCCAA AGAAGGACTA CCTTGAGCCA TACCTTCCAG TTGAGGACTA CCCAAATCAG 
CTAAACCCTA CCAAACTCTT TTCCCTGTCA AGCACTGATG TAAGACAAGG CCAGAATCCG 
TTTGCCTCTG AGTTTCAGCA TCAGAGACAG CACAGTGATG ATAGTCCATT TTCTGTGTAT 
AACAACTCTA CATGTTCATC AAAGTCTCAG AGTGTTGGGA GCTACTACCG AAGTTTGCAA 
GATTTGCCTA ATAATGTTAG CAATCAAAAC AAAACTAGAA CCAGCACAGT CTTTGATCCC 
AACCATGATG GCCAAGCTCA TTTCCGTTAC TACTGCATCA CTGCCCAACA GCCATACCTG 
GAGTCATCTT CTCACTGTCG GATGGTAGAT GAACAAAGTT CTGAAATGGG AAAAGCACAA 
GGTGCCACAG ATAGAAGCTT TGTAGGTTCT CAAAAGGTTA TAAAAGCAAA GCATCCCCAA 
CTGTACCTTA GTTTACCCGA GAACAAGAGA TCCAATGGTT ACAACAAACA GTCCATCAGT 
CCAGCTTCAG CTCAAGCTGT GTCCCCGATA TCAAATAATT TGATTTCAAA GCACAGTTCT 
GTGGAAAGAG AGACTCAAAA AAAGAAAGAT AAGCCTCAGC CTTCCAGGCA CATTCCAAAC 
CACCAGACTG AGCAGAACTC CATGTACACT TACCAACATG ACAATCCATG GATCAGCAAG 
CAGGATCTGA AGATCTGTCC CCACAAAACC CCCTTGTTGC ATTCATTGGC CCAGGAGAGT 
AAGAAAATGG CAGAAAATTC GATTACAACA GCCTCCAAAG GAGGTAGTCA TGAATCAAGT 
GATGCCAGCA GTGGAAAGCT GGGAAGACGA AGTGACCGCT ATGCCACAAC TTTACGCAAA 
GAGATTCAAA AGAAGAGGGC TCAACTTCAA AAAAGCAGAA GCGCAGCTAC CTTGACTTGC 
TCAACTGAAG TTGAGGAGGA CTCTGACATA TGGAAGTCCA ATGAAACCTC CACATCCTCT 
TCTGATGGTT CCTTTACTAA CACGTACAAG GATCATCTGA AGGAAGCCCA AGCCAAAGTT 
CTACAGGCTA CATCATTCAT GAGGAAAGAT CTGGAGCTTC CTGGGAATGA AGTTGTGTCT 
GCTCAACTTC CCAAAAGACC TGGGCAAGTC ACCCGCATTG GAGGTCGTAA ACGCTTTCCC 
ATGGATAAAA AAATACATTC CTTCTCTGAA CCGGACAAGA TCAATGAGGT CGGAGTTGAA 
GATAAAGCTG GCGGGTCTTT TGCAGACCGA TACAAGTTCT TTGAAGGTTC TGGCAAAGCG 
GTTGTCCAAA AGCCCATTCC AAAGCAGTCC CTGCTTGGTC CTACTGTAGA CCACAGTGAG 
AGAAAGACTG GAGAAGCTGA AAGTATCCCT CGAGTACTGT TAAAGCAAAG TAAATCCAGC 
CATTCTGACC TTAATGCAGA TGAGCAACAA AGGCTTGGTA CATTTGCAGA ATATGAGGCT 
ACATGGAACT TGCAGAAGAA GCAAGTGGAA AGAAGAAACA CAGGCAGATA CCATTCAGCT 
GAAAATATTT TGGACTCAGG CTTGGAGGAA TCCAACAGTG CACTGTGTGT GCATGAAAGA 
TCACGCTCCT CACCTTCTGC TGACTTCTAT GGACAGAAAC TTCCTTTGCC ACCCAGAAAG 
TTACCTTTGG ATTCTCCACC TGAAGCCAAA GACCAACAGG AGGCTCATAT TTCCAGTGCA 
TCTGAAAAGG AACACAATGC ACTTAATATT TCCAAGTCTC AAGAGTCCAT CACAATGAGC 
ATTCTGGACA ATTCAGTTGA ACCTCTTGGA TACAACCAGA AATCCAGATC TGACACCAAG 
CAGCCAGCAC CGATTACTTC ACAACATCTC CAGGTTCCCA TGTCAGAGCA ACTTCTTCCC 
AAAAACAATG GTCTTATACC ACAACACAAA GAACTTGAAA CCACCTGCCC AATCATGGCT 
TCCCTAGAAG TCAGGATTCA GACCTTTTCA CCAGAGCCAA CACAAGAACG CCTGAGACTC 
AATACTTCAG AACCTCTGGA CGGAGACATG CATGCACAAG TGGATGAGCA TAAATCAGAG 
AGGATGTTAT CTCCTATTCC ACATAATTCA GCTGGTTCCC CTATCCCTCC CAAGCAGAAG 
GCTGACCTGA CGGTAACCTC AGCCAGCAGA ACACGTTCTC CTTCTCCTCA GTTCTTTCCA 
CAGAGACTCA CGGATGAACC TCCTGCAAAT GTGTACAAAA AGGATCCATG CAGTAGTAAG 
GTGGAAGAGC CAAACACTCC TGGCAGAAAG GTCCCCATAC AGATTGTTTG TACAGAAAAT 
GGTTCAGAGA GAGACATCCG AAACTATCTG CTGCACTCAG AATCATCTGA AGAGCCTCAA 
ACTGGCCAGC TAAAAACCCT GGAATCTTCA GAGCAATGTC ATGCACCATT CATCGCATAC 
TCAAACCAGA TAGAAGAACC AATATTAGAC CCTTTCAAAC CAATGAGGAG CAACGGTGTG 
AGCGAACACT CGGAGGACGA TCTGAAGCGA GAGGAGCTGG CCAGAGATAT AATGGGGAAA 
GACAGGAGTC TGGTGGACAT TCTGGACCAG AGAAAGATGA AGACCACCAT GGATCTGATG 
GAGGGAATAT TTCCACAGGG CGAGCAGATA CTGGAGGGAG CGCAGCAGAG GAGGAAAGCG 
GCGTCCAAAC AGACGTCTCC TAAAAATGCA CAGCAGAGGA TGGAGGAGAG TCTGTCTTCA 
TCAGTCTCGT TGGTCAGCAG TTCATCATAT TACAGCACAT CTGCACCCAA AGCAGAGCTG 
CTCATTAAGA TGAAGGACAT GCAGGAGCAG GACTCAGAGG ATGAGCTCAC TACTGACCTC 
TCCAGCAAGA AGCAAGAGCT GATGGACAGT TTGAGCAAGA AGCTGCAGGT TCTGAGAGAG 
GCTCGTGAAA GCCTGCAGGA GGACGTTCAG GATAATAACG CTCTGGGAGA GGAAGTGGAA 
GCCACAGTCC AGAGCGTCTG CAGAGCCAAT GAGCTGGAGA AGTTTCGCAT GTTTGTTGGA 
GATCTGGATA AAGTGGTCAG TCTGCTGCTG TCTCTGTCTG GACGGCTGGC ACGTGTGGAA 
AACGCACTCG ATAACCTGGA GGAGGGAACA TCCGCTGACG AGAAGCAAAC GTTGACGGAG 
AAGCGCAGGC TGCTGATTGG TCAACATGAA GACGCCAAAG AGCTGAAGGA GAATCTGGAC 
CGCCGCGAGC GCGTGGTTTA TGAGATCCTG ACGGGATACT TCAGGGAAGA GCAGCTGGCA 
GATTACAGAC ACTTCGTTAA AATGAAATCG GCGCTTATTA TAGAGCAGCG CAAACTGGAG 
GACAAGATAA AACTGGGAGA AGAGCAGCTG AAGTGTCTGA AGGAGAGTCT GCCGCTCGAA 
CAGAGACTCC TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_684470.7
CDS	96..4373
Translation	MVDIVTQTMPAESDVHVARNFLTKILRSSMRRNESSCRPHSWHSAKSSESSNPNDSKSEPNPAPDPTWQTKCDPGSPTKEFTSCWGQTNLRQASCQFSSVRNMESVEPSSHAFSKEQPLTSKPNGGADHEVLESAISKPALPGNAFSGTADHGAPRGANPTEGVFYRGDLTVPSDCHRYLQIPIGNGGRVSPSVEEQSSSRLSSTARSNHRPVWHIPENKMSSSEAPPAPPLRSDSFTATRVHEKSLVGLFPEGHTVYPLQRPPCRAVDRQLEDVRHSYNSPPKKDYLEPYLPVEDYPNQLNPTKLFSLSSTDVRQGQNPFASEFQHQRQHSDDSPFSVYNNSTCSSKSQSVGSYYRSLQDLPNNVSNQNKTRTSTVFDPNHDGQAHFRYYCITAQQPYLESSSHCRMVDEQSSEMGKAQGATDRSFVGSQKVIKAKHPQLYLSLPENKRSNGYNKQSISPASAQAVSPISNNLISKHSSVERETQKKKDKPQPSRHIPNHQTEQNSMYTYQHDNPWISKQDLKICPHKTPLLHSLAQESKKMAENSITTASKGGSHESSDASSGKLGRRSDRYATTLRKEIQKKRAQLQKSRSAATLTCSTEVEEDSDIWKSNETSTSSSDGSFTNTYKDHLKEAQAKVLQATSFMRKDLELPGNEVVSAQLPKRPGQVTRIGGRKRFPMDKKIHSFSEPDKINEVGVEDKAGGSFADRYKFFEGSGKAVVQKPIPKQSLLGPTVDHSERKTGEAESIPRVLLKQSKSSHSDLNADEQQRLGTFAEYEATWNLQKKQVERRNTGRYHSAENILDSGLEESNSALCVHERSRSSPSADFYGQKLPLPPRKLPLDSPPEAKDQQEAHISSASEKEHNALNISKSQESITMSILDNSVEPLGYNQKSRSDTKQPAPITSQHLQVPMSEQLLPKNNGLIPQHKELETTCPIMASLEVRIQTFSPEPTQERLRLNTSEPLDGDMHAQVDEHKSERMLSPIPHNSAGSPIPPKQKADLTVTSASRTRSPSPQFFPQRLTDEPPANVYKKDPCSSKVEEPNTPGRKVPIQIVCTENGSERDIRNYLLHSESSEEPQTGQLKTLESSEQCHAPFIAYSNQIEEPILDPFKPMRSNGVSEHSEDDLKREELARDIMGKDRSLVDILDQRKMKTTMDLMEGIFPQGEQILEGAQQRRKAASKQTSPKNAQQRMEESLSSSVSLVSSSSYYSTSAPKAELLIKMKDMQEQDSEDELTTDLSSKKQELMDSLSKKLQVLREARESLQEDVQDNNALGEEVEATVQSVCRANELEKFRMFVGDLDKVVSLLLSLSGRLARVENALDNLEEGTSADEKQTLTEKRRLLIGQHEDAKELKENLDRRERVVYEILTGYFREEQLADYRHFVKMKSALIIEQRKLEDKIKLGEEQLKCLKESLPLEQRLLY

Target ORF information:

RefSeq Version	XM_679378.10
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2b (shroom2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_679378.10

ORF Insert Sequence:

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAGGAATG AATCGTCATG TAGACCCCAT 
TCATGGCACT CAGCTAAATC CTCGGAAAGC TCCAACCCAA ACGACTCCAA ATCTGAACCC 
AACCCAGCAC CTGACCCCAC TTGGCAGACA AAATGTGATC CTGGGTCACC CACAAAAGAA 
TTCACCAGCT GTTGGGGACA GACCAATCTG CGACAAGCAT CCTGCCAGTT CAGCTCGGTC 
AGAAATATGG AAAGCGTAGA GCCCTCATCT CATGCTTTCT CAAAAGAACA ACCTTTAACA 
AGCAAACCCA ATGGAGGAGC AGATCACGAG GTGCTTGAAA GTGCAATCAG CAAACCTGCT 
TTGCCAGGCA ATGCATTTTC AGGCACAGCT GATCATGGTG CACCTCGGGG TGCCAATCCC 
ACAGAAGGGG TGTTTTACAG AGGCGATCTA ACAGTCCCGA GTGACTGCCA CAGATACCTG 
CAGATCCCCA TAGGCAATGG GGGACGAGTG AGTCCCAGTG TTGAGGAGCA ATCTAGCTCC 
AGACTCTCCT CTACTGCGAG ATCAAACCAT AGACCTGTGT GGCATATTCC AGAGAATAAG 
ATGTCCAGTT CTGAAGCTCC TCCTGCTCCT CCTTTGCGGA GTGATAGTTT CACTGCCACC 
AGAGTTCATG AGAAGAGCTT AGTGGGATTG TTCCCTGAGG GACACACTGT ATATCCACTA 
CAGAGACCTC CCTGTAGAGC TGTGGACAGA CAATTAGAGG ACGTGAGACA CAGCTACAAC 
TCGCCTCCAA AGAAGGACTA CCTTGAGCCA TACCTTCCAG TTGAGGACTA CCCAAATCAG 
CTAAACCCTA CCAAACTCTT TTCCCTGTCA AGCACTGATG TAAGACAAGG CCAGAATCCG 
TTTGCCTCTG AGTTTCAGCA TCAGAGACAG CACAGTGATG ATAGTCCATT TTCTGTGTAT 
AACAACTCTA CATGTTCATC AAAGTCTCAG AGTGTTGGGA GCTACTACCG AAGTTTGCAA 
GATTTGCCTA ATAATGTTAG CAATCAAAAC AAAACTAGAA CCAGCACAGT CTTTGATCCC 
AACCATGATG GCCAAGCTCA TTTCCGTTAC TACTGCATCA CTGCCCAACA GCCATACCTG 
GAGTCATCTT CTCACTGTCG GATGGTAGAT GAACAAAGTT CTGAAATGGG AAAAGCACAA 
GGTGCCACAG ATAGAAGCTT TGTAGGTTCT CAAAAGGTTA TAAAAGCAAA GCATCCCCAA 
CTGTACCTTA GTTTACCCGA GAACAAGAGA TCCAATGGTT ACAACAAACA GTCCATCAGT 
CCAGCTTCAG CTCAAGCTGT GTCCCCGATA TCAAATAATT TGATTTCAAA GCACAGTTCT 
GTGGAAAGAG AGACTCAAAA AAAGAAAGAT AAGCCTCAGC CTTCCAGGCA CATTCCAAAC 
CACCAGACTG AGCAGAACTC CATGTACACT TACCAACATG ACAATCCATG GATCAGCAAG 
CAGGATCTGA AGATCTGTCC CCACAAAACC CCCTTGTTGC ATTCATTGGC CCAGGAGAGT 
AAGAAAATGG CAGAAAATTC GATTACAACA GCCTCCAAAG GAGGTAGTCA TGAATCAAGT 
GATGCCAGCA GTGGAAAGCT GGGAAGACGA AGTGACCGCT ATGCCACAAC TTTACGCAAA 
GAGATTCAAA AGAAGAGGGC TCAACTTCAA AAAAGCAGAA GCGCAGCTAC CTTGACTTGC 
TCAACTGAAG TTGAGGAGGA CTCTGACATA TGGAAGTCCA ATGAAACCTC CACATCCTCT 
TCTGATGGTT CCTTTACTAA CACGTACAAG GATCATCTGA AGGAAGCCCA AGCCAAAGTT 
CTACAGGCTA CATCATTCAT GAGGAAAGAT CTGGAGCTTC CTGGGAATGA AGTTGTGTCT 
GCTCAACTTC CCAAAAGACC TGGGCAAGTC ACCCGCATTG GAGGTCGTAA ACGCTTTCCC 
ATGGATAAAA AAATACATTC CTTCTCTGAA CCGGACAAGA TCAATGAGGT CGGAGTTGAA 
GATAAAGCTG GCGGGTCTTT TGCAGACCGA TACAAGTTCT TTGAAGGTTC TGGCAAAGCG 
GTTGTCCAAA AGCCCATTCC AAAGCAGTCC CTGCTTGGTC CTACTGTAGA CCACAGTGAG 
AGAAAGACTG GAGAAGCTGA AAGTATCCCT CGAGTACTGT TAAAGCAAAG TAAATCCAGC 
CATTCTGACC TTAATGCAGA TGAGCAACAA AGGCTTGGTA CATTTGCAGA ATATGAGGCT 
ACATGGAACT TGCAGAAGAA GCAAGTGGAA AGAAGAAACA CAGGCAGATA CCATTCAGCT 
GAAAATATTT TGGACTCAGG CTTGGAGGAA TCCAACAGTG CACTGTGTGT GCATGAAAGA 
TCACGCTCCT CACCTTCTGC TGACTTCTAT GGACAGAAAC TTCCTTTGCC ACCCAGAAAG 
TTACCTTTGG ATTCTCCACC TGAAGCCAAA GACCAACAGG AGGCTCATAT TTCCAGTGCA 
TCTGAAAAGG AACACAATGC ACTTAATATT TCCAAGTCTC AAGAGTCCAT CACAATGAGC 
ATTCTGGACA ATTCAGTTGA ACCTCTTGGA TACAACCAGA AATCCAGATC TGACACCAAG 
CAGCCAGCAC CGATTACTTC ACAACATCTC CAGGTTCCCA TGTCAGAGCA ACTTCTTCCC 
AAAAACAATG GTCTTATACC ACAACACAAA GAACTTGAAA CCACCTGCCC AATCATGGCT 
TCCCTAGAAG TCAGGATTCA GACCTTTTCA CCAGAGCCAA CACAAGAACG CCTGAGACTC 
AATACTTCAG AACCTCTGGA CGGAGACATG CATGCACAAG TGGATGAGCA TAAATCAGAG 
AGGATGTTAT CTCCTATTCC ACATAATTCA GCTGGTTCCC CTATCCCTCC CAAGCAGAAG 
GCTGACCTGA CGGTAACCTC AGCCAGCAGA ACACGTTCTC CTTCTCCTCA GTTCTTTCCA 
CAGAGACTCA CGGATGAACC TCCTGCAAAT GTGTACAAAA AGGATCCATG CAGTAGTAAG 
GTGGAAGAGC CAAACACTCC TGGCAGAAAG GTCCCCATAC AGATTGTTTG TACAGAAAAT 
GGTTCAGAGA GAGACATCCG AAACTATCTG CTGCACTCAG AATCATCTGA AGAGCCTCAA 
ACTGGCCAGC TAAAAACCCT GGAATCTTCA GAGCAATGTC ATGCACCATT CATCGCATAC 
TCAAACCAGA TAGAAGAACC AATATTAGAC CCTTTCAAAC CAATGAGGAG CAACGGTGTG 
AGCGAACACT CGGAGGACGA TCTGAAGCGA GAGGAGCTGG CCAGAGATAT AATGGGGAAA 
GACAGGAGTC TGGTGGACAT TCTGGACCAG AGAAAGATGA AGACCACCAT GGATCTGATG 
GAGGGAATAT TTCCACAGGG CGAGCAGATA CTGGAGGGAG CGCAGCAGAG GAGGAAAGCG 
GCGTCCAAAC AGACGTCTCC TAAAAATGCA CAGCAGAGGA TGGAGGAGAG TCTGTCTTCA 
TCAGTCTCGT TGGTCAGCAG TTCATCATAT TACAGCACAT CTGCACCCAA AGCAGAGCTG 
CTCATTAAGA TGAAGGACAT GCAGGAGCAG GACTCAGAGG ATGAGCTCAC TACTGACCTC 
TCCAGCAAGA AGCAAGAGCT GATGGACAGT TTGAGCAAGA AGCTGCAGGT TCTGAGAGAG 
GCTCGTGAAA GCCTGCAGGA GGACGTTCAG GATAATAACG CTCTGGGAGA GGAAGTGGAA 
GCCACAGTCC AGAGCGTCTG CAGAGCCAAT GAGCTGGAGA AGTTTCGCAT GTTTGTTGGA 
GATCTGGATA AAGTGGTCAG TCTGCTGCTG TCTCTGTCTG GACGGCTGGC ACGTGTGGAA 
AACGCACTCG ATAACCTGGA GGAGGGAACA TCCGCTGACG AGAAGCAAAC GTTGACGGAG 
AAGCGCAGGC TGCTGATTGG TCAACATGAA GACGCCAAAG AGCTGAAGGA GAATCTGGAC 
CGCCGCGAGC GCGTGGTTTA TGAGATCCTG ACGGGATACT TCAGGGAAGA GCAGCTGGCA 
GATTACAGAC ACTTCGTTAA AATGAAATCG GCGCTTATTA TAGAGCAGCG CAAACTGGAG 
GACAAGATAA AACTGGGAGA AGAGCAGCTG AAGTGTCTGA AGGAGAGTCT GCCGCTCGAA 
CAGAGACTCC TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2b ( XM_021479922.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335597.1 Danio rerio shroom family member 2b (shroom2b), transcript variant X4, mRNA.

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CloneID	ODa148075
Clone ID Related Accession (Same CDS sequence)	XM_021479922.1
Accession Version	XM_021479922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein Shroom2-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCG TAGAGCCCTC ATCTCATGCT TTCTCAAAAG AACAACCTTT AACAAGCAAA 
CCCAATGGAG GAGCAGATCA CGAGGTGCTT GAAAGTGCAA TCAGCAAACC TGCTTTGCCA 
GGCAATGCAT TTTCAGGCAC AGCTGATCAT GGTGCACCTC GGGGTGCCAA TCCCACAGAA 
GGGGTGTTTT ACAGAGGCGA TCTAACAGTC CCGAGTGACT GCCACAGATA CCTGCAGATC 
CCCATAGGCA ATGGGGGACG AGTGAGTCCC AGTGTTGAGG AGCAATCTAG CTCCAGACTC 
TCCTCTACTG CGAGATCAAA CCATAGACCT GTGTGGCATA TTCCAGAGAA TAAGATGTCC 
AGTTCTGAAG CTCCTCCTGC TCCTCCTTTG CGGAGTGATA GTTTCACTGC CACCAGAGTT 
CATGAGAAGA GCTTAGTGGG ATTGTTCCCT GAGGGACACA CTGTATATCC ACTACAGAGA 
CCTCCCTGTA GAGCTGTGGA CAGACAATTA GAGGACGTGA GACACAGCTA CAACTCGCCT 
CCAAAGAAGG ACTACCTTGA GCCATACCTT CCAGTTGAGG ACTACCCAAA TCAGCTAAAC 
CCTACCAAAC TCTTTTCCCT GTCAAGCACT GATGTAAGAC AAGGCCAGAA TCCGTTTGCC 
TCTGAGTTTC AGCATCAGAG ACAGCACAGT GATGATAGTC CATTTTCTGT GTATAACAAC 
TCTACATGTT CATCAAAGTC TCAGAGTGTT GGGAGCTACT ACCGAAGTTT GCAAGATTTG 
CCTAATAATG TTAGCAATCA AAACAAAACT AGAACCAGCA CAGTCTTTGA TCCCAACCAT 
GATGGCCAAG CTCATTTCCG TTACTACTGC ATCACTGCCC AACAGCCATA CCTGGAGTCA 
TCTTCTCACT GTCGGATGGT AGATGAACAA AGTTCTGAAA TGGGAAAAGC ACAAGGTGCC 
ACAGATAGAA GCTTTGTAGG TTCTCAAAAG GTTATAAAAG CAAAGCATCC CCAACTGTAC 
CTTAGTTTAC CCGAGAACAA GAGATCCAAT GGTTACAACA AACAGTCCAT CAGTCCAGCT 
TCAGCTCAAG CTGTGTCCCC GATATCAAAT AATTTGATTT CAAAGCACAG TTCTGTGGAA 
AGAGAGACTC AAAAAAAGAA AGATAAGCCT CAGCCTTCCA GGCACATTCC AAACCACCAG 
ACTGAGCAGA ACTCCATGTA CACTTACCAA CATGACAATC CATGGATCAG CAAGCAGGAT 
CTGAAGATCT GTCCCCACAA AACCCCCTTG TTGCATTCAT TGGCCCAGGA GAGTAAGAAA 
ATGGCAGAAA ATTCGATTAC AACAGCCTCC AAAGGAGGTA GTCATGAATC AAGTGATGCC 
AGCAGTGGAA AGCTGGGAAG ACGAAGTGAC CGCTATGCCA CAACTTTACG CAAAGAGATT 
CAAAAGAAGA GGGCTCAACT TCAAAAAAGC AGAAGCGCAG CTACCTTGAC TTGCTCAACT 
GAAGTTGAGG AGGACTCTGA CATATGGAAG TCCAATGAAA CCTCCACATC CTCTTCTGAT 
GGTTCCTTTA CTAACACGTA CAAGGATCAT CTGAAGGAAG CCCAAGCCAA AGTTCTACAG 
GCTACATCAT TCATGAGGAA AGATCTGGAG CTTCCTGGGA ATGAAGTTGT GTCTGCTCAA 
CTTCCCAAAA GACCTGGGCA AGTCACCCGC ATTGGAGGTC GTAAACGCTT TCCCATGGAT 
AAAAAAATAC ATTCCTTCTC TGAACCGGAC AAGATCAATG AGGTCGGAGT TGAAGATAAA 
GCTGGCGGGT CTTTTGCAGA CCGATACAAG TTCTTTGAAG GTTCTGGCAA AGCGGTTGTC 
CAAAAGCCCA TTCCAAAGCA GTCCCTGCTT GGTCCTACTG TAGACCACAG TGAGAGAAAG 
ACTGGAGAAG CTGAAAGTAT CCCTCGAGTA CTGTTAAAGC AAAGTAAATC CAGCCATTCT 
GACCTTAATG CAGATGAGCA ACAAAGGCTT GGTACATTTG CAGAATATGA GGCTACATGG 
AACTTGCAGA AGAAGCAAGT GGAAAGAAGA AACACAGGCA GATACCATTC AGCTGAAAAT 
ATTTTGGACT CAGGCTTGGA GGAATCCAAC AGTGCACTGT GTGTGCATGA AAGATCACGC 
TCCTCACCTT CTGCTGACTT CTATGGACAG AAACTTCCTT TGCCACCCAG AAAGTTACCT 
TTGGATTCTC CACCTGAAGC CAAAGACCAA CAGGAGGCTC ATATTTCCAG TGCATCTGAA 
AAGGAACACA ATGCACTTAA TATTTCCAAG TCTCAAGAGT CCATCACAAT GAGCATTCTG 
GACAATTCAG TTGAACCTCT TGGATACAAC CAGAAATCCA GATCTGACAC CAAGCAGCCA 
GCACCGATTA CTTCACAACA TCTCCAGGTT CCCATGTCAG AGCAACTTCT TCCCAAAAAC 
AATGGTCTTA TACCACAACA CAAAGAACTT GAAACCACCT GCCCAATCAT GGCTTCCCTA 
GAAGTCAGGA TTCAGACCTT TTCACCAGAG CCAACACAAG AACGCCTGAG ACTCAATACT 
TCAGAACCTC TGGACGGAGA CATGCATGCA CAAGTGGATG AGCATAAATC AGAGAGGATG 
TTATCTCCTA TTCCACATAA TTCAGCTGGT TCCCCTATCC CTCCCAAGCA GAAGGCTGAC 
CTGACGGTAA CCTCAGCCAG CAGAACACGT TCTCCTTCTC CTCAGTTCTT TCCACAGAGA 
CTCACGGATG AACCTCCTGC AAATGTGTAC AAAAAGGATC CATGCAGTAG TAAGGTGGAA 
GAGCCAAACA CTCCTGGCAG AAAGGTCCCC ATACAGATTG TTTGTACAGA AAATGGTTCA 
GAGAGAGACA TCCGAAACTA TCTGCTGCAC TCAGAATCAT CTGAAGAGCC TCAAACTGGC 
CAGCTAAAAA CCCTGGAATC TTCAGAGCAA TGTCATGCAC CATTCATCGC ATACTCAAAC 
CAGATAGAAG AACCAATATT AGACCCTTTC AAACCAATGA GGAGCAACGG TGTGAGCGAA 
CACTCGGAGG ACGATCTGAA GCGAGAGGAG CTGGCCAGAG ATATAATGGG GAAAGACAGG 
AGTCTGGTGG ACATTCTGGA CCAGAGAAAG ATGAAGACCA CCATGGATCT GATGGAGGGA 
ATATTTCCAC AGGGCGAGCA GATACTGGAG GGAGCGCAGC AGAGGAGGAA AGCGGCGTCC 
AAACAGACGT CTCCTAAAAA TGCACAGCAG AGGATGGAGG AGAGTCTGTC TTCATCAGTC 
TCGTTGGTCA GCAGTTCATC ATATTACAGC ACATCTGCAC CCAAAGCAGA GCTGCTCATT 
AAGATGAAGG ACATGCAGGA GCAGGACTCA GAGGATGAGC TCACTACTGA CCTCTCCAGC 
AAGAAGCAAG AGCTGATGGA CAGTTTGAGC AAGAAGCTGC AGGTTCTGAG AGAGGCTCGT 
GAAAGCCTGC AGGAGGACGT TCAGGATAAT AACGCTCTGG GAGAGGAAGT GGAAGCCACA 
GTCCAGAGCG TCTGCAGAGC CAATGAGCTG GAGAAGTTTC GCATGTTTGT TGGAGATCTG 
GATAAAGTGG TCAGTCTGCT GCTGTCTCTG TCTGGACGGC TGGCACGTGT GGAAAACGCA 
CTCGATAACC TGGAGGAGGG AACATCCGCT GACGAGAAGC AAACGTTGAC GGAGAAGCGC 
AGGCTGCTGA TTGGTCAACA TGAAGACGCC AAAGAGCTGA AGGAGAATCT GGACCGCCGC 
GAGCGCGTGG TTTATGAGAT CCTGACGGGA TACTTCAGGG AAGAGCAGCT GGCAGATTAC 
AGACACTTCG TTAAAATGAA ATCGGCGCTT ATTATAGAGC AGCGCAAACT GGAGGACAAG 
ATAAAACTGG GAGAAGAGCA GCTGAAGTGT CTGAAGGAGA GTCTGCCGCT CGAACAGAGA 
CTCCTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335597.1
CDS	323..4294
Translation	MESVEPSSHAFSKEQPLTSKPNGGADHEVLESAISKPALPGNAFSGTADHGAPRGANPTEGVFYRGDLTVPSDCHRYLQIPIGNGGRVSPSVEEQSSSRLSSTARSNHRPVWHIPENKMSSSEAPPAPPLRSDSFTATRVHEKSLVGLFPEGHTVYPLQRPPCRAVDRQLEDVRHSYNSPPKKDYLEPYLPVEDYPNQLNPTKLFSLSSTDVRQGQNPFASEFQHQRQHSDDSPFSVYNNSTCSSKSQSVGSYYRSLQDLPNNVSNQNKTRTSTVFDPNHDGQAHFRYYCITAQQPYLESSSHCRMVDEQSSEMGKAQGATDRSFVGSQKVIKAKHPQLYLSLPENKRSNGYNKQSISPASAQAVSPISNNLISKHSSVERETQKKKDKPQPSRHIPNHQTEQNSMYTYQHDNPWISKQDLKICPHKTPLLHSLAQESKKMAENSITTASKGGSHESSDASSGKLGRRSDRYATTLRKEIQKKRAQLQKSRSAATLTCSTEVEEDSDIWKSNETSTSSSDGSFTNTYKDHLKEAQAKVLQATSFMRKDLELPGNEVVSAQLPKRPGQVTRIGGRKRFPMDKKIHSFSEPDKINEVGVEDKAGGSFADRYKFFEGSGKAVVQKPIPKQSLLGPTVDHSERKTGEAESIPRVLLKQSKSSHSDLNADEQQRLGTFAEYEATWNLQKKQVERRNTGRYHSAENILDSGLEESNSALCVHERSRSSPSADFYGQKLPLPPRKLPLDSPPEAKDQQEAHISSASEKEHNALNISKSQESITMSILDNSVEPLGYNQKSRSDTKQPAPITSQHLQVPMSEQLLPKNNGLIPQHKELETTCPIMASLEVRIQTFSPEPTQERLRLNTSEPLDGDMHAQVDEHKSERMLSPIPHNSAGSPIPPKQKADLTVTSASRTRSPSPQFFPQRLTDEPPANVYKKDPCSSKVEEPNTPGRKVPIQIVCTENGSERDIRNYLLHSESSEEPQTGQLKTLESSEQCHAPFIAYSNQIEEPILDPFKPMRSNGVSEHSEDDLKREELARDIMGKDRSLVDILDQRKMKTTMDLMEGIFPQGEQILEGAQQRRKAASKQTSPKNAQQRMEESLSSSVSLVSSSSYYSTSAPKAELLIKMKDMQEQDSEDELTTDLSSKKQELMDSLSKKLQVLREARESLQEDVQDNNALGEEVEATVQSVCRANELEKFRMFVGDLDKVVSLLLSLSGRLARVENALDNLEEGTSADEKQTLTEKRRLLIGQHEDAKELKENLDRRERVVYEILTGYFREEQLADYRHFVKMKSALIIEQRKLEDKIKLGEEQLKCLKESLPLEQRLLY

Target ORF information:

RefSeq Version	XM_021479922.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio shroom family member 2b (shroom2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479922.1

ORF Insert Sequence:

ATGGAAAGCG TAGAGCCCTC ATCTCATGCT TTCTCAAAAG AACAACCTTT AACAAGCAAA 
CCCAATGGAG GAGCAGATCA CGAGGTGCTT GAAAGTGCAA TCAGCAAACC TGCTTTGCCA 
GGCAATGCAT TTTCAGGCAC AGCTGATCAT GGTGCACCTC GGGGTGCCAA TCCCACAGAA 
GGGGTGTTTT ACAGAGGCGA TCTAACAGTC CCGAGTGACT GCCACAGATA CCTGCAGATC 
CCCATAGGCA ATGGGGGACG AGTGAGTCCC AGTGTTGAGG AGCAATCTAG CTCCAGACTC 
TCCTCTACTG CGAGATCAAA CCATAGACCT GTGTGGCATA TTCCAGAGAA TAAGATGTCC 
AGTTCTGAAG CTCCTCCTGC TCCTCCTTTG CGGAGTGATA GTTTCACTGC CACCAGAGTT 
CATGAGAAGA GCTTAGTGGG ATTGTTCCCT GAGGGACACA CTGTATATCC ACTACAGAGA 
CCTCCCTGTA GAGCTGTGGA CAGACAATTA GAGGACGTGA GACACAGCTA CAACTCGCCT 
CCAAAGAAGG ACTACCTTGA GCCATACCTT CCAGTTGAGG ACTACCCAAA TCAGCTAAAC 
CCTACCAAAC TCTTTTCCCT GTCAAGCACT GATGTAAGAC AAGGCCAGAA TCCGTTTGCC 
TCTGAGTTTC AGCATCAGAG ACAGCACAGT GATGATAGTC CATTTTCTGT GTATAACAAC 
TCTACATGTT CATCAAAGTC TCAGAGTGTT GGGAGCTACT ACCGAAGTTT GCAAGATTTG 
CCTAATAATG TTAGCAATCA AAACAAAACT AGAACCAGCA CAGTCTTTGA TCCCAACCAT 
GATGGCCAAG CTCATTTCCG TTACTACTGC ATCACTGCCC AACAGCCATA CCTGGAGTCA 
TCTTCTCACT GTCGGATGGT AGATGAACAA AGTTCTGAAA TGGGAAAAGC ACAAGGTGCC 
ACAGATAGAA GCTTTGTAGG TTCTCAAAAG GTTATAAAAG CAAAGCATCC CCAACTGTAC 
CTTAGTTTAC CCGAGAACAA GAGATCCAAT GGTTACAACA AACAGTCCAT CAGTCCAGCT 
TCAGCTCAAG CTGTGTCCCC GATATCAAAT AATTTGATTT CAAAGCACAG TTCTGTGGAA 
AGAGAGACTC AAAAAAAGAA AGATAAGCCT CAGCCTTCCA GGCACATTCC AAACCACCAG 
ACTGAGCAGA ACTCCATGTA CACTTACCAA CATGACAATC CATGGATCAG CAAGCAGGAT 
CTGAAGATCT GTCCCCACAA AACCCCCTTG TTGCATTCAT TGGCCCAGGA GAGTAAGAAA 
ATGGCAGAAA ATTCGATTAC AACAGCCTCC AAAGGAGGTA GTCATGAATC AAGTGATGCC 
AGCAGTGGAA AGCTGGGAAG ACGAAGTGAC CGCTATGCCA CAACTTTACG CAAAGAGATT 
CAAAAGAAGA GGGCTCAACT TCAAAAAAGC AGAAGCGCAG CTACCTTGAC TTGCTCAACT 
GAAGTTGAGG AGGACTCTGA CATATGGAAG TCCAATGAAA CCTCCACATC CTCTTCTGAT 
GGTTCCTTTA CTAACACGTA CAAGGATCAT CTGAAGGAAG CCCAAGCCAA AGTTCTACAG 
GCTACATCAT TCATGAGGAA AGATCTGGAG CTTCCTGGGA ATGAAGTTGT GTCTGCTCAA 
CTTCCCAAAA GACCTGGGCA AGTCACCCGC ATTGGAGGTC GTAAACGCTT TCCCATGGAT 
AAAAAAATAC ATTCCTTCTC TGAACCGGAC AAGATCAATG AGGTCGGAGT TGAAGATAAA 
GCTGGCGGGT CTTTTGCAGA CCGATACAAG TTCTTTGAAG GTTCTGGCAA AGCGGTTGTC 
CAAAAGCCCA TTCCAAAGCA GTCCCTGCTT GGTCCTACTG TAGACCACAG TGAGAGAAAG 
ACTGGAGAAG CTGAAAGTAT CCCTCGAGTA CTGTTAAAGC AAAGTAAATC CAGCCATTCT 
GACCTTAATG CAGATGAGCA ACAAAGGCTT GGTACATTTG CAGAATATGA GGCTACATGG 
AACTTGCAGA AGAAGCAAGT GGAAAGAAGA AACACAGGCA GATACCATTC AGCTGAAAAT 
ATTTTGGACT CAGGCTTGGA GGAATCCAAC AGTGCACTGT GTGTGCATGA AAGATCACGC 
TCCTCACCTT CTGCTGACTT CTATGGACAG AAACTTCCTT TGCCACCCAG AAAGTTACCT 
TTGGATTCTC CACCTGAAGC CAAAGACCAA CAGGAGGCTC ATATTTCCAG TGCATCTGAA 
AAGGAACACA ATGCACTTAA TATTTCCAAG TCTCAAGAGT CCATCACAAT GAGCATTCTG 
GACAATTCAG TTGAACCTCT TGGATACAAC CAGAAATCCA GATCTGACAC CAAGCAGCCA 
GCACCGATTA CTTCACAACA TCTCCAGGTT CCCATGTCAG AGCAACTTCT TCCCAAAAAC 
AATGGTCTTA TACCACAACA CAAAGAACTT GAAACCACCT GCCCAATCAT GGCTTCCCTA 
GAAGTCAGGA TTCAGACCTT TTCACCAGAG CCAACACAAG AACGCCTGAG ACTCAATACT 
TCAGAACCTC TGGACGGAGA CATGCATGCA CAAGTGGATG AGCATAAATC AGAGAGGATG 
TTATCTCCTA TTCCACATAA TTCAGCTGGT TCCCCTATCC CTCCCAAGCA GAAGGCTGAC 
CTGACGGTAA CCTCAGCCAG CAGAACACGT TCTCCTTCTC CTCAGTTCTT TCCACAGAGA 
CTCACGGATG AACCTCCTGC AAATGTGTAC AAAAAGGATC CATGCAGTAG TAAGGTGGAA 
GAGCCAAACA CTCCTGGCAG AAAGGTCCCC ATACAGATTG TTTGTACAGA AAATGGTTCA 
GAGAGAGACA TCCGAAACTA TCTGCTGCAC TCAGAATCAT CTGAAGAGCC TCAAACTGGC 
CAGCTAAAAA CCCTGGAATC TTCAGAGCAA TGTCATGCAC CATTCATCGC ATACTCAAAC 
CAGATAGAAG AACCAATATT AGACCCTTTC AAACCAATGA GGAGCAACGG TGTGAGCGAA 
CACTCGGAGG ACGATCTGAA GCGAGAGGAG CTGGCCAGAG ATATAATGGG GAAAGACAGG 
AGTCTGGTGG ACATTCTGGA CCAGAGAAAG ATGAAGACCA CCATGGATCT GATGGAGGGA 
ATATTTCCAC AGGGCGAGCA GATACTGGAG GGAGCGCAGC AGAGGAGGAA AGCGGCGTCC 
AAACAGACGT CTCCTAAAAA TGCACAGCAG AGGATGGAGG AGAGTCTGTC TTCATCAGTC 
TCGTTGGTCA GCAGTTCATC ATATTACAGC ACATCTGCAC CCAAAGCAGA GCTGCTCATT 
AAGATGAAGG ACATGCAGGA GCAGGACTCA GAGGATGAGC TCACTACTGA CCTCTCCAGC 
AAGAAGCAAG AGCTGATGGA CAGTTTGAGC AAGAAGCTGC AGGTTCTGAG AGAGGCTCGT 
GAAAGCCTGC AGGAGGACGT TCAGGATAAT AACGCTCTGG GAGAGGAAGT GGAAGCCACA 
GTCCAGAGCG TCTGCAGAGC CAATGAGCTG GAGAAGTTTC GCATGTTTGT TGGAGATCTG 
GATAAAGTGG TCAGTCTGCT GCTGTCTCTG TCTGGACGGC TGGCACGTGT GGAAAACGCA 
CTCGATAACC TGGAGGAGGG AACATCCGCT GACGAGAAGC AAACGTTGAC GGAGAAGCGC 
AGGCTGCTGA TTGGTCAACA TGAAGACGCC AAAGAGCTGA AGGAGAATCT GGACCGCCGC 
GAGCGCGTGG TTTATGAGAT CCTGACGGGA TACTTCAGGG AAGAGCAGCT GGCAGATTAC 
AGACACTTCG TTAAAATGAA ATCGGCGCTT ATTATAGAGC AGCGCAAACT GGAGGACAAG 
ATAAAACTGG GAGAAGAGCA GCTGAAGTGT CTGAAGGAGA GTCTGCCGCT CGAACAGAGA 
CTCCTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2b ( XM_017358378.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213867.1 Danio rerio shroom family member 2b (shroom2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa54398
Clone ID Related Accession (Same CDS sequence)	XM_017358378.1
Accession Version	XM_017358378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGGTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAAGGTGGA AGAGCCAAAC ACTCCTGGCA GAAAGGTCCC CATACAGATT 
GTTTGTACAG AAAATGGTTC AGAGAGAGAC ATCCGAAACT ATCTGCTGCA CTCAGAATCA 
TCTGAAGAGC CTCAAACTGA CCAGCTAAAA ACCCTGGAAT CTTCAGAGCA ATGTCATTCA 
CCATTCATCG CATACTCAAA CCAGATAGAA GAACCAATAT TAGACCCCAC CGAACCAATG 
AGGAGCAACG GTGTGAGCGA ACACTCGGAG GACGATCTGA AGCGAGAGGA GCTGGCCAGA 
GATATAATGG GGAAAGACAG GAGTCTGGTG GACATTCTGG ACCAGAGAAA GATGAAGACC 
ACCATGGATC TGATGGAGGG AATATTTCCA CAGGGCGAGC AGATACTGGA GGGAGCGCAG 
CAGAGGAGGA AAGCGGCGTC CAAACAGACG TCTCCTAAAA ATGCACAGCA GAGGATGGAG 
GAGAGTCTGT CTTCATCAGT CTCGTTGGTC AGCAGTTCAT CATATTACAG CACATCTGCA 
CCCAAAGCAG AGCTGCTCAT TAAGATGAAG GACATGCAGG AGCAGGACTC AGAGGATGAG 
CTCACTACTG ACCTCTCCAG CAAGAAGCAA GAGCTGATGG ACAGTTTGAG CAAGAAGCTG 
CAGGTTCTGA GAGAGGCTCG TGAAAGCCTG CAGGAGGACG TTCAGGATAA TAACGCTCTG 
GGAGAGGAAG TGGAAGCCAC AGTCCAGAGC GTCTGCAGAG CCAATGAGCT GGAGAAGTTT 
CGCATGTTTG TTGGAGATCT GGATAAAGTG GTCAGTCTGC TGCTGTCTCT GTCTGGACGG 
CTGGCACGTG TGGAAAACGC ACTCGATAAC CTGGAGGAGG GAACATCCGC TGACGAGAAG 
CAAACGTTGA CGGAGAAGCG CAGGCTGCTG ATTGGTCAAC ATGAAGACGC CAAAGAGCTG 
AAGGAGAATC TGGACCGCCG CGAGCGCGTG GTTTATGAGA TCCTGACGGG ATACTTCAGG 
GAAGAGCAGC TGGCAGATTA CAGACACTTC GTTAAAATGA AATCGGCGCT TATTATAGAG 
CAGCGCAAAC TGGAGGACAA GATAAAACTG GGAGAAGAGC AGCTGAAGTG TCTGAAGGAG 
AGTCTGCCGC TCGAACAGAG ACTCCTGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213867.1
CDS	105..4397
Translation	MVDIVTQTMPAESDVHVARNFLTKILRSSMRKNRFKGRNESSCRPHSWHSAKSSESSNPNDSKSEPNPAPDPTWQTKCDPGSPTKEFTSCWGQTNLRQASCQFSSVRNMESVEPSSHAFSKEQPLTSKPNGGADHEVLESAISKPALPGNAFSGTADHGAPRGANPTEGVFYRGDLTVPSDCHRYLQIPIGNGGRVSPSVEEQSSSRLSSTARSNHRPVWHIPENKMSSSEAPPAPPLRSDSFTATRVHEKSLVGLFPEGHTVYPLQRPPCRAVDRQLEDVRHSYNSPPKKDYLEPYLPVEDYPNQLNPTKLFSLSSTDVRQGQNPFASEFQHQRQHSDDSPFSVYNNSTCSSKSQSVGSYYRSLQDLPNNVSNQNKTRTSTVFDPNHDGQAHFRYYCITAQQPYLESSSHCRMVDEQSSEMGKAQGATDRSFVGSQKVIKAKHPQLYLSLPENKRSNGYNKQSISPASAQAVSPISNNLISKHSSVERETQKKKDKPQPSRHIPNHQTEQNSMYTYQHDNPWISKQDLKICPHKTPLLHSLAQESKKMAENSITTASKGGSHESSDASSGKLGRRSDRYATTLRKEIQKKRAQLQKSRSAATLTCSTEVEEDSDIWKSNETSTSSSDGSFTNTYKDHLKEAQAKVLQATSFMRKDLELPGNEVVSAQLPKRPGQVTRIGGRKRFPMDKKIHSFSEPDKINEVGVEDKAGGSFADRYKFFEGSGKAVVQKPIPKQSLLGPTVDHSERKTGEGESIPRVLLKQSKSSHSDLNADEQQRLGTFAEYEATWNLQKKQVERRNTGRYHSAENILDSGLEESNSALCVHERSRSSPSADFYGQKLPLPPRKLPLDSPPEAKDQQEAHISSASEKEHNALNISKSQESITMSILDNSVEPLGYNQKSRSDTKQPAPITSQHLQVPMSEQLLPKNNGLIPQHKELETTCPIMASLEVRIQTFSPEPTQERLRLNTSEPLDGDMHAQVDEHKSERMLSPIPHNSAGSPIPPKQKADLTVTSASRTRSPSPQFFPQRLTDEPPANVYKKDPCSKVEEPNTPGRKVPIQIVCTENGSERDIRNYLLHSESSEEPQTDQLKTLESSEQCHSPFIAYSNQIEEPILDPTEPMRSNGVSEHSEDDLKREELARDIMGKDRSLVDILDQRKMKTTMDLMEGIFPQGEQILEGAQQRRKAASKQTSPKNAQQRMEESLSSSVSLVSSSSYYSTSAPKAELLIKMKDMQEQDSEDELTTDLSSKKQELMDSLSKKLQVLREARESLQEDVQDNNALGEEVEATVQSVCRANELEKFRMFVGDLDKVVSLLLSLSGRLARVENALDNLEEGTSADEKQTLTEKRRLLIGQHEDAKELKENLDRRERVVYEILTGYFREEQLADYRHFVKMKSALIIEQRKLEDKIKLGEEQLKCLKESLPLEQRLLY

Target ORF information:

RefSeq Version	XM_017358378.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2b (shroom2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358378.1

ORF Insert Sequence:

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGGTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAAGGTGGA AGAGCCAAAC ACTCCTGGCA GAAAGGTCCC CATACAGATT 
GTTTGTACAG AAAATGGTTC AGAGAGAGAC ATCCGAAACT ATCTGCTGCA CTCAGAATCA 
TCTGAAGAGC CTCAAACTGA CCAGCTAAAA ACCCTGGAAT CTTCAGAGCA ATGTCATTCA 
CCATTCATCG CATACTCAAA CCAGATAGAA GAACCAATAT TAGACCCCAC CGAACCAATG 
AGGAGCAACG GTGTGAGCGA ACACTCGGAG GACGATCTGA AGCGAGAGGA GCTGGCCAGA 
GATATAATGG GGAAAGACAG GAGTCTGGTG GACATTCTGG ACCAGAGAAA GATGAAGACC 
ACCATGGATC TGATGGAGGG AATATTTCCA CAGGGCGAGC AGATACTGGA GGGAGCGCAG 
CAGAGGAGGA AAGCGGCGTC CAAACAGACG TCTCCTAAAA ATGCACAGCA GAGGATGGAG 
GAGAGTCTGT CTTCATCAGT CTCGTTGGTC AGCAGTTCAT CATATTACAG CACATCTGCA 
CCCAAAGCAG AGCTGCTCAT TAAGATGAAG GACATGCAGG AGCAGGACTC AGAGGATGAG 
CTCACTACTG ACCTCTCCAG CAAGAAGCAA GAGCTGATGG ACAGTTTGAG CAAGAAGCTG 
CAGGTTCTGA GAGAGGCTCG TGAAAGCCTG CAGGAGGACG TTCAGGATAA TAACGCTCTG 
GGAGAGGAAG TGGAAGCCAC AGTCCAGAGC GTCTGCAGAG CCAATGAGCT GGAGAAGTTT 
CGCATGTTTG TTGGAGATCT GGATAAAGTG GTCAGTCTGC TGCTGTCTCT GTCTGGACGG 
CTGGCACGTG TGGAAAACGC ACTCGATAAC CTGGAGGAGG GAACATCCGC TGACGAGAAG 
CAAACGTTGA CGGAGAAGCG CAGGCTGCTG ATTGGTCAAC ATGAAGACGC CAAAGAGCTG 
AAGGAGAATC TGGACCGCCG CGAGCGCGTG GTTTATGAGA TCCTGACGGG ATACTTCAGG 
GAAGAGCAGC TGGCAGATTA CAGACACTTC GTTAAAATGA AATCGGCGCT TATTATAGAG 
CAGCGCAAAC TGGAGGACAA GATAAAACTG GGAGAAGAGC AGCTGAAGTG TCTGAAGGAG 
AGTCTGCCGC TCGAACAGAG ACTCCTGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2b ( XM_005172940.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172997.1 Danio rerio shroom family member 2b (shroom2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17404
Clone ID Related Accession (Same CDS sequence)	XM_005172940.3
Accession Version	XM_005172940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGGTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAGTAAGGT GGAAGAGCCA AACACTCCTG GCAGAAAGGT CCCCATACAG 
ATTGTTTGTA CAGAAAATGG TTCAGAGAGA GACATCCGAA ACTATCTGCT GCACTCAGAA 
TCATCTGAAG AGCCTCAAAC TGACCAGCTA AAAACCCTGG AATCTTCAGA GCAATGTCAT 
TCACCATTCA TCGCATACTC AAACCAGATA GAAGAACCAA TATTAGACCC CACCGAACCA 
ATGAGGAGCA ACGGTGTGAG CGAACACTCG GAGGACGATC TGAAGCGAGA GGAGCTGGCC 
AGAGATATAA TGGGGAAAGA CAGGAGTCTG GTGGACATTC TGGACCAGAG AAAGATGAAG 
ACCACCATGG ATCTGATGGA GGGAATATTT CCACAGGGCG AGCAGATACT GGAGGGAGCG 
CAGCAGAGGA GGAAAGCGGC GTCCAAACAG ACGTCTCCTA AAAATGCACA GCAGAGGATG 
GAGGAGAGTC TGTCTTCATC AGTCTCGTTG GTCAGCAGTT CATCATATTA CAGCACATCT 
GCACCCAAAG CAGAGCTGCT CATTAAGATG AAGGACATGC AGGAGCAGGA CTCAGAGGAT 
GAGCTCACTA CTGACCTCTC CAGCAAGAAG CAAGAGCTGA TGGACAGTTT GAGCAAGAAG 
CTGCAGGTTC TGAGAGAGGC TCGTGAAAGC CTGCAGGAGG ACGTTCAGGA TAATAACGCT 
CTGGGAGAGG AAGTGGAAGC CACAGTCCAG AGCGTCTGCA GAGCCAATGA GCTGGAGAAG 
TTTCGCATGT TTGTTGGAGA TCTGGATAAA GTGGTCAGTC TGCTGCTGTC TCTGTCTGGA 
CGGCTGGCAC GTGTGGAAAA CGCACTCGAT AACCTGGAGG AGGGAACATC CGCTGACGAG 
AAGCAAACGT TGACGGAGAA GCGCAGGCTG CTGATTGGTC AACATGAAGA CGCCAAAGAG 
CTGAAGGAGA ATCTGGACCG CCGCGAGCGC GTGGTTTATG AGATCCTGAC GGGATACTTC 
AGGGAAGAGC AGCTGGCAGA TTACAGACAC TTCGTTAAAA TGAAATCGGC GCTTATTATA 
GAGCAGCGCA AACTGGAGGA CAAGATAAAA CTGGGAGAAG AGCAGCTGAA GTGTCTGAAG 
GAGAGTCTGC CGCTCGAACA GAGACTCCTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172997.1
CDS	105..4400
Translation	MVDIVTQTMPAESDVHVARNFLTKILRSSMRKNRFKGRNESSCRPHSWHSAKSSESSNPNDSKSEPNPAPDPTWQTKCDPGSPTKEFTSCWGQTNLRQASCQFSSVRNMESVEPSSHAFSKEQPLTSKPNGGADHEVLESAISKPALPGNAFSGTADHGAPRGANPTEGVFYRGDLTVPSDCHRYLQIPIGNGGRVSPSVEEQSSSRLSSTARSNHRPVWHIPENKMSSSEAPPAPPLRSDSFTATRVHEKSLVGLFPEGHTVYPLQRPPCRAVDRQLEDVRHSYNSPPKKDYLEPYLPVEDYPNQLNPTKLFSLSSTDVRQGQNPFASEFQHQRQHSDDSPFSVYNNSTCSSKSQSVGSYYRSLQDLPNNVSNQNKTRTSTVFDPNHDGQAHFRYYCITAQQPYLESSSHCRMVDEQSSEMGKAQGATDRSFVGSQKVIKAKHPQLYLSLPENKRSNGYNKQSISPASAQAVSPISNNLISKHSSVERETQKKKDKPQPSRHIPNHQTEQNSMYTYQHDNPWISKQDLKICPHKTPLLHSLAQESKKMAENSITTASKGGSHESSDASSGKLGRRSDRYATTLRKEIQKKRAQLQKSRSAATLTCSTEVEEDSDIWKSNETSTSSSDGSFTNTYKDHLKEAQAKVLQATSFMRKDLELPGNEVVSAQLPKRPGQVTRIGGRKRFPMDKKIHSFSEPDKINEVGVEDKAGGSFADRYKFFEGSGKAVVQKPIPKQSLLGPTVDHSERKTGEGESIPRVLLKQSKSSHSDLNADEQQRLGTFAEYEATWNLQKKQVERRNTGRYHSAENILDSGLEESNSALCVHERSRSSPSADFYGQKLPLPPRKLPLDSPPEAKDQQEAHISSASEKEHNALNISKSQESITMSILDNSVEPLGYNQKSRSDTKQPAPITSQHLQVPMSEQLLPKNNGLIPQHKELETTCPIMASLEVRIQTFSPEPTQERLRLNTSEPLDGDMHAQVDEHKSERMLSPIPHNSAGSPIPPKQKADLTVTSASRTRSPSPQFFPQRLTDEPPANVYKKDPCSSKVEEPNTPGRKVPIQIVCTENGSERDIRNYLLHSESSEEPQTDQLKTLESSEQCHSPFIAYSNQIEEPILDPTEPMRSNGVSEHSEDDLKREELARDIMGKDRSLVDILDQRKMKTTMDLMEGIFPQGEQILEGAQQRRKAASKQTSPKNAQQRMEESLSSSVSLVSSSSYYSTSAPKAELLIKMKDMQEQDSEDELTTDLSSKKQELMDSLSKKLQVLREARESLQEDVQDNNALGEEVEATVQSVCRANELEKFRMFVGDLDKVVSLLLSLSGRLARVENALDNLEEGTSADEKQTLTEKRRLLIGQHEDAKELKENLDRRERVVYEILTGYFREEQLADYRHFVKMKSALIIEQRKLEDKIKLGEEQLKCLKESLPLEQRLLY

Target ORF information:

RefSeq Version	XM_005172940.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2b (shroom2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172940.3

ORF Insert Sequence:

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAAAAACC GCTTCAAAGG GAGGAATGAA 
TCGTCATGTA GACCCCATTC ATGGCACTCA GCTAAATCCT CGGAAAGCTC CAACCCAAAC 
GACTCCAAAT CTGAACCCAA CCCAGCACCT GACCCCACTT GGCAGACAAA ATGTGATCCT 
GGGTCACCCA CAAAAGAATT CACCAGCTGT TGGGGACAGA CCAATCTGCG ACAAGCATCC 
TGCCAGTTCA GCTCGGTCAG AAATATGGAA AGCGTAGAGC CCTCATCTCA TGCTTTCTCA 
AAAGAACAAC CTTTAACAAG CAAACCCAAT GGAGGAGCAG ATCACGAGGT GCTTGAAAGT 
GCAATCAGCA AACCTGCTTT GCCAGGCAAT GCATTTTCAG GCACAGCTGA TCATGGTGCA 
CCTCGGGGTG CCAATCCCAC AGAAGGGGTG TTTTACAGAG GCGATCTAAC AGTCCCGAGT 
GACTGCCACA GATACCTGCA GATCCCCATA GGCAATGGGG GACGAGTGAG TCCCAGTGTT 
GAGGAGCAAT CTAGCTCCAG ACTCTCCTCT ACTGCGAGAT CAAACCATAG ACCTGTGTGG 
CATATTCCAG AGAATAAGAT GTCCAGTTCT GAAGCTCCTC CTGCTCCTCC TTTGCGGAGT 
GATAGTTTCA CTGCCACCAG AGTTCATGAG AAGAGCTTAG TGGGATTGTT CCCTGAGGGA 
CACACTGTAT ATCCACTACA GAGACCTCCC TGTAGAGCTG TGGACAGACA ATTAGAGGAC 
GTGAGACACA GCTACAACTC GCCTCCAAAG AAGGACTACC TTGAGCCATA CCTTCCAGTT 
GAGGACTACC CAAATCAGCT AAACCCTACC AAACTCTTTT CCCTGTCAAG CACTGATGTA 
AGACAAGGCC AGAATCCGTT TGCCTCTGAG TTTCAGCATC AGAGACAGCA CAGTGATGAT 
AGTCCATTTT CTGTGTATAA CAACTCTACA TGTTCATCAA AGTCTCAGAG TGTTGGGAGC 
TACTACCGAA GTTTGCAAGA TTTGCCTAAT AATGTTAGCA ATCAAAACAA AACTAGAACC 
AGCACAGTCT TTGATCCCAA CCATGATGGC CAAGCTCATT TCCGTTACTA CTGCATCACT 
GCCCAACAGC CATACCTGGA GTCATCTTCT CACTGTCGGA TGGTAGATGA ACAAAGTTCT 
GAAATGGGAA AAGCACAAGG TGCCACAGAT AGAAGCTTTG TAGGTTCTCA AAAGGTTATA 
AAAGCAAAGC ATCCCCAACT GTACCTTAGT TTACCCGAGA ACAAGAGATC CAATGGTTAC 
AACAAACAGT CCATCAGTCC AGCTTCAGCT CAAGCTGTGT CCCCGATATC AAATAATTTG 
ATTTCAAAGC ACAGTTCTGT GGAAAGAGAG ACTCAAAAAA AGAAAGATAA GCCTCAGCCT 
TCCAGGCACA TTCCAAACCA CCAGACTGAG CAGAACTCCA TGTACACTTA CCAACATGAC 
AATCCATGGA TCAGCAAGCA GGATCTGAAG ATCTGTCCCC ACAAAACCCC CTTGTTGCAT 
TCATTGGCCC AGGAGAGTAA GAAAATGGCA GAAAATTCGA TTACAACAGC CTCCAAAGGA 
GGTAGTCATG AATCAAGTGA TGCCAGCAGT GGAAAGCTGG GAAGACGAAG TGACCGCTAT 
GCCACAACTT TACGCAAAGA GATTCAAAAG AAGAGGGCTC AACTTCAAAA AAGCAGAAGC 
GCAGCTACCT TGACTTGCTC AACTGAAGTT GAGGAGGACT CTGACATATG GAAGTCCAAT 
GAAACCTCCA CATCCTCTTC TGATGGTTCC TTTACTAACA CGTACAAGGA TCATCTGAAG 
GAAGCCCAAG CCAAAGTTCT ACAGGCTACA TCATTCATGA GGAAAGATCT GGAGCTTCCT 
GGGAATGAAG TTGTGTCTGC TCAACTTCCC AAAAGACCTG GGCAAGTCAC CCGCATTGGA 
GGTCGTAAAC GCTTTCCCAT GGATAAAAAA ATACATTCCT TCTCTGAACC GGACAAGATC 
AATGAGGTCG GAGTTGAAGA TAAAGCTGGC GGGTCTTTTG CAGACCGATA CAAGTTCTTT 
GAAGGTTCTG GCAAAGCGGT TGTCCAAAAG CCCATTCCAA AGCAGTCCCT GCTTGGTCCT 
ACTGTAGACC ACAGTGAGAG AAAGACTGGA GAAGGTGAAA GTATCCCTCG AGTACTGTTA 
AAGCAAAGTA AATCCAGCCA TTCTGACCTT AATGCAGATG AGCAACAAAG GCTTGGTACA 
TTTGCAGAAT ATGAGGCTAC ATGGAACTTG CAGAAGAAGC AAGTGGAAAG AAGAAACACA 
GGCAGATACC ATTCAGCTGA AAATATTTTG GACTCAGGCT TGGAGGAATC CAACAGTGCA 
CTGTGTGTGC ATGAAAGATC ACGCTCCTCA CCTTCTGCTG ACTTCTATGG ACAGAAACTT 
CCTTTGCCAC CCAGAAAGTT ACCTTTGGAT TCTCCACCTG AAGCCAAAGA CCAACAGGAG 
GCTCATATTT CCAGTGCATC TGAAAAGGAA CACAATGCAC TTAATATTTC CAAGTCTCAA 
GAGTCCATCA CAATGAGCAT TCTGGACAAT TCAGTTGAAC CTCTTGGATA CAACCAGAAA 
TCCAGATCTG ACACCAAGCA GCCAGCACCG ATTACTTCAC AACATCTCCA GGTTCCCATG 
TCAGAGCAAC TTCTTCCCAA AAACAATGGT CTTATACCAC AACACAAAGA ACTTGAAACC 
ACCTGCCCAA TCATGGCTTC CCTAGAAGTC AGGATTCAGA CCTTTTCACC AGAGCCAACA 
CAAGAACGCC TGAGACTCAA TACTTCAGAA CCTCTGGACG GAGACATGCA TGCACAAGTG 
GATGAGCATA AATCAGAGAG GATGTTATCT CCTATTCCAC ATAATTCAGC TGGTTCCCCT 
ATCCCTCCCA AGCAGAAGGC TGACCTGACG GTAACCTCAG CCAGCAGAAC ACGTTCTCCT 
TCTCCTCAGT TCTTTCCACA GAGACTCACG GATGAACCTC CTGCAAATGT GTACAAAAAG 
GATCCATGCA GTAGTAAGGT GGAAGAGCCA AACACTCCTG GCAGAAAGGT CCCCATACAG 
ATTGTTTGTA CAGAAAATGG TTCAGAGAGA GACATCCGAA ACTATCTGCT GCACTCAGAA 
TCATCTGAAG AGCCTCAAAC TGACCAGCTA AAAACCCTGG AATCTTCAGA GCAATGTCAT 
TCACCATTCA TCGCATACTC AAACCAGATA GAAGAACCAA TATTAGACCC CACCGAACCA 
ATGAGGAGCA ACGGTGTGAG CGAACACTCG GAGGACGATC TGAAGCGAGA GGAGCTGGCC 
AGAGATATAA TGGGGAAAGA CAGGAGTCTG GTGGACATTC TGGACCAGAG AAAGATGAAG 
ACCACCATGG ATCTGATGGA GGGAATATTT CCACAGGGCG AGCAGATACT GGAGGGAGCG 
CAGCAGAGGA GGAAAGCGGC GTCCAAACAG ACGTCTCCTA AAAATGCACA GCAGAGGATG 
GAGGAGAGTC TGTCTTCATC AGTCTCGTTG GTCAGCAGTT CATCATATTA CAGCACATCT 
GCACCCAAAG CAGAGCTGCT CATTAAGATG AAGGACATGC AGGAGCAGGA CTCAGAGGAT 
GAGCTCACTA CTGACCTCTC CAGCAAGAAG CAAGAGCTGA TGGACAGTTT GAGCAAGAAG 
CTGCAGGTTC TGAGAGAGGC TCGTGAAAGC CTGCAGGAGG ACGTTCAGGA TAATAACGCT 
CTGGGAGAGG AAGTGGAAGC CACAGTCCAG AGCGTCTGCA GAGCCAATGA GCTGGAGAAG 
TTTCGCATGT TTGTTGGAGA TCTGGATAAA GTGGTCAGTC TGCTGCTGTC TCTGTCTGGA 
CGGCTGGCAC GTGTGGAAAA CGCACTCGAT AACCTGGAGG AGGGAACATC CGCTGACGAG 
AAGCAAACGT TGACGGAGAA GCGCAGGCTG CTGATTGGTC AACATGAAGA CGCCAAAGAG 
CTGAAGGAGA ATCTGGACCG CCGCGAGCGC GTGGTTTATG AGATCCTGAC GGGATACTTC 
AGGGAAGAGC AGCTGGCAGA TTACAGACAC TTCGTTAAAA TGAAATCGGC GCTTATTATA 
GAGCAGCGCA AACTGGAGGA CAAGATAAAA CTGGGAGAAG AGCAGCTGAA GTGTCTGAAG 
GAGAGTCTGC CGCTCGAACA GAGACTCCTG TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2b ( XM_679378.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_684470.6 Danio rerio shroom family member 2b (shroom2b), transcript variant X3, mRNA.

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CloneID	ODa17406
Clone ID Related Accession (Same CDS sequence)	XM_679378.9
Accession Version	XM_679378.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein Shroom2-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679378.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAGGAATG AATCGTCATG TAGACCCCAT 
TCATGGCACT CAGCTAAATC CTCGGAAAGC TCCAACCCAA ACGACTCCAA ATCTGAACCC 
AACCCAGCAC CTGACCCCAC TTGGCAGACA AAATGTGATC CTGGGTCACC CACAAAAGAA 
TTCACCAGCT GTTGGGGACA GACCAATCTG CGACAAGCAT CCTGCCAGTT CAGCTCGGTC 
AGAAATATGG AAAGCGTAGA GCCCTCATCT CATGCTTTCT CAAAAGAACA ACCTTTAACA 
AGCAAACCCA ATGGAGGAGC AGATCACGAG GTGCTTGAAA GTGCAATCAG CAAACCTGCT 
TTGCCAGGCA ATGCATTTTC AGGCACAGCT GATCATGGTG CACCTCGGGG TGCCAATCCC 
ACAGAAGGGG TGTTTTACAG AGGCGATCTA ACAGTCCCGA GTGACTGCCA CAGATACCTG 
CAGATCCCCA TAGGCAATGG GGGACGAGTG AGTCCCAGTG TTGAGGAGCA ATCTAGCTCC 
AGACTCTCCT CTACTGCGAG ATCAAACCAT AGACCTGTGT GGCATATTCC AGAGAATAAG 
ATGTCCAGTT CTGAAGCTCC TCCTGCTCCT CCTTTGCGGA GTGATAGTTT CACTGCCACC 
AGAGTTCATG AGAAGAGCTT AGTGGGATTG TTCCCTGAGG GACACACTGT ATATCCACTA 
CAGAGACCTC CCTGTAGAGC TGTGGACAGA CAATTAGAGG ACGTGAGACA CAGCTACAAC 
TCGCCTCCAA AGAAGGACTA CCTTGAGCCA TACCTTCCAG TTGAGGACTA CCCAAATCAG 
CTAAACCCTA CCAAACTCTT TTCCCTGTCA AGCACTGATG TAAGACAAGG CCAGAATCCG 
TTTGCCTCTG AGTTTCAGCA TCAGAGACAG CACAGTGATG ATAGTCCATT TTCTGTGTAT 
AACAACTCTA CATGTTCATC AAAGTCTCAG AGTGTTGGGA GCTACTACCG AAGTTTGCAA 
GATTTGCCTA ATAATGTTAG CAATCAAAAC AAAACTAGAA CCAGCACAGT CTTTGATCCC 
AACCATGATG GCCAAGCTCA TTTCCGTTAC TACTGCATCA CTGCCCAACA GCCATACCTG 
GAGTCATCTT CTCACTGTCG GATGGTAGAT GAACAAAGTT CTGAAATGGG AAAAGCACAA 
GGTGCCACAG ATAGAAGCTT TGTAGGTTCT CAAAAGGTTA TAAAAGCAAA GCATCCCCAA 
CTGTACCTTA GTTTACCCGA GAACAAGAGA TCCAATGGTT ACAACAAACA GTCCATCAGT 
CCAGCTTCAG CTCAAGCTGT GTCCCCGATA TCAAATAATT TGATTTCAAA GCACAGTTCT 
GTGGAAAGAG AGACTCAAAA AAAGAAAGAT AAGCCTCAGC CTTCCAGGCA CATTCCAAAC 
CACCAGACTG AGCAGAACTC CATGTACACT TACCAACATG ACAATCCATG GATCAGCAAG 
CAGGATCTGA AGATCTGTCC CCACAAAACC CCCTTGTTGC ATTCATTGGC CCAGGAGAGT 
AAGAAAATGG CAGAAAATTC GATTACAACA GCCTCCAAAG GAGGTAGTCA TGAATCAAGT 
GATGCCAGCA GTGGAAAGCT GGGAAGACGA AGTGACCGCT ATGCCACAAC TTTACGCAAA 
GAGATTCAAA AGAAGAGGGC TCAACTTCAA AAAAGCAGAA GCGCAGCTAC CTTGACTTGC 
TCAACTGAAG TTGAGGAGGA CTCTGACATA TGGAAGTCCA ATGAAACCTC CACATCCTCT 
TCTGATGGTT CCTTTACTAA CACGTACAAG GATCATCTGA AGGAAGCCCA AGCCAAAGTT 
CTACAGGCTA CATCATTCAT GAGGAAAGAT CTGGAGCTTC CTGGGAATGA AGTTGTGTCT 
GCTCAACTTC CCAAAAGACC TGGGCAAGTC ACCCGCATTG GAGGTCGTAA ACGCTTTCCC 
ATGGATAAAA AAATACATTC CTTCTCTGAA CCGGACAAGA TCAATGAGGT CGGAGTTGAA 
GATAAAGCTG GCGGGTCTTT TGCAGACCGA TACAAGTTCT TTGAAGGTTC TGGCAAAGCG 
GTTGTCCAAA AGCCCATTCC AAAGCAGTCC CTGCTTGGTC CTACTGTAGA CCACAGTGAG 
AGAAAGACTG GAGAAGGTGA AAGTATCCCT CGAGTACTGT TAAAGCAAAG TAAATCCAGC 
CATTCTGACC TTAATGCAGA TGAGCAACAA AGGCTTGGTA CATTTGCAGA ATATGAGGCT 
ACATGGAACT TGCAGAAGAA GCAAGTGGAA AGAAGAAACA CAGGCAGATA CCATTCAGCT 
GAAAATATTT TGGACTCAGG CTTGGAGGAA TCCAACAGTG CACTGTGTGT GCATGAAAGA 
TCACGCTCCT CACCTTCTGC TGACTTCTAT GGACAGAAAC TTCCTTTGCC ACCCAGAAAG 
TTACCTTTGG ATTCTCCACC TGAAGCCAAA GACCAACAGG AGGCTCATAT TTCCAGTGCA 
TCTGAAAAGG AACACAATGC ACTTAATATT TCCAAGTCTC AAGAGTCCAT CACAATGAGC 
ATTCTGGACA ATTCAGTTGA ACCTCTTGGA TACAACCAGA AATCCAGATC TGACACCAAG 
CAGCCAGCAC CGATTACTTC ACAACATCTC CAGGTTCCCA TGTCAGAGCA ACTTCTTCCC 
AAAAACAATG GTCTTATACC ACAACACAAA GAACTTGAAA CCACCTGCCC AATCATGGCT 
TCCCTAGAAG TCAGGATTCA GACCTTTTCA CCAGAGCCAA CACAAGAACG CCTGAGACTC 
AATACTTCAG AACCTCTGGA CGGAGACATG CATGCACAAG TGGATGAGCA TAAATCAGAG 
AGGATGTTAT CTCCTATTCC ACATAATTCA GCTGGTTCCC CTATCCCTCC CAAGCAGAAG 
GCTGACCTGA CGGTAACCTC AGCCAGCAGA ACACGTTCTC CTTCTCCTCA GTTCTTTCCA 
CAGAGACTCA CGGATGAACC TCCTGCAAAT GTGTACAAAA AGGATCCATG CAGTAGTAAG 
GTGGAAGAGC CAAACACTCC TGGCAGAAAG GTCCCCATAC AGATTGTTTG TACAGAAAAT 
GGTTCAGAGA GAGACATCCG AAACTATCTG CTGCACTCAG AATCATCTGA AGAGCCTCAA 
ACTGACCAGC TAAAAACCCT GGAATCTTCA GAGCAATGTC ATTCACCATT CATCGCATAC 
TCAAACCAGA TAGAAGAACC AATATTAGAC CCCACCGAAC CAATGAGGAG CAACGGTGTG 
AGCGAACACT CGGAGGACGA TCTGAAGCGA GAGGAGCTGG CCAGAGATAT AATGGGGAAA 
GACAGGAGTC TGGTGGACAT TCTGGACCAG AGAAAGATGA AGACCACCAT GGATCTGATG 
GAGGGAATAT TTCCACAGGG CGAGCAGATA CTGGAGGGAG CGCAGCAGAG GAGGAAAGCG 
GCGTCCAAAC AGACGTCTCC TAAAAATGCA CAGCAGAGGA TGGAGGAGAG TCTGTCTTCA 
TCAGTCTCGT TGGTCAGCAG TTCATCATAT TACAGCACAT CTGCACCCAA AGCAGAGCTG 
CTCATTAAGA TGAAGGACAT GCAGGAGCAG GACTCAGAGG ATGAGCTCAC TACTGACCTC 
TCCAGCAAGA AGCAAGAGCT GATGGACAGT TTGAGCAAGA AGCTGCAGGT TCTGAGAGAG 
GCTCGTGAAA GCCTGCAGGA GGACGTTCAG GATAATAACG CTCTGGGAGA GGAAGTGGAA 
GCCACAGTCC AGAGCGTCTG CAGAGCCAAT GAGCTGGAGA AGTTTCGCAT GTTTGTTGGA 
GATCTGGATA AAGTGGTCAG TCTGCTGCTG TCTCTGTCTG GACGGCTGGC ACGTGTGGAA 
AACGCACTCG ATAACCTGGA GGAGGGAACA TCCGCTGACG AGAAGCAAAC GTTGACGGAG 
AAGCGCAGGC TGCTGATTGG TCAACATGAA GACGCCAAAG AGCTGAAGGA GAATCTGGAC 
CGCCGCGAGC GCGTGGTTTA TGAGATCCTG ACGGGATACT TCAGGGAAGA GCAGCTGGCA 
GATTACAGAC ACTTCGTTAA AATGAAATCG GCGCTTATTA TAGAGCAGCG CAAACTGGAG 
GACAAGATAA AACTGGGAGA AGAGCAGCTG AAGTGTCTGA AGGAGAGTCT GCCGCTCGAA 
CAGAGACTCC TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_684470.6
CDS	105..4382
Translation	MVDIVTQTMPAESDVHVARNFLTKILRSSMRRNESSCRPHSWHSAKSSESSNPNDSKSEPNPAPDPTWQTKCDPGSPTKEFTSCWGQTNLRQASCQFSSVRNMESVEPSSHAFSKEQPLTSKPNGGADHEVLESAISKPALPGNAFSGTADHGAPRGANPTEGVFYRGDLTVPSDCHRYLQIPIGNGGRVSPSVEEQSSSRLSSTARSNHRPVWHIPENKMSSSEAPPAPPLRSDSFTATRVHEKSLVGLFPEGHTVYPLQRPPCRAVDRQLEDVRHSYNSPPKKDYLEPYLPVEDYPNQLNPTKLFSLSSTDVRQGQNPFASEFQHQRQHSDDSPFSVYNNSTCSSKSQSVGSYYRSLQDLPNNVSNQNKTRTSTVFDPNHDGQAHFRYYCITAQQPYLESSSHCRMVDEQSSEMGKAQGATDRSFVGSQKVIKAKHPQLYLSLPENKRSNGYNKQSISPASAQAVSPISNNLISKHSSVERETQKKKDKPQPSRHIPNHQTEQNSMYTYQHDNPWISKQDLKICPHKTPLLHSLAQESKKMAENSITTASKGGSHESSDASSGKLGRRSDRYATTLRKEIQKKRAQLQKSRSAATLTCSTEVEEDSDIWKSNETSTSSSDGSFTNTYKDHLKEAQAKVLQATSFMRKDLELPGNEVVSAQLPKRPGQVTRIGGRKRFPMDKKIHSFSEPDKINEVGVEDKAGGSFADRYKFFEGSGKAVVQKPIPKQSLLGPTVDHSERKTGEGESIPRVLLKQSKSSHSDLNADEQQRLGTFAEYEATWNLQKKQVERRNTGRYHSAENILDSGLEESNSALCVHERSRSSPSADFYGQKLPLPPRKLPLDSPPEAKDQQEAHISSASEKEHNALNISKSQESITMSILDNSVEPLGYNQKSRSDTKQPAPITSQHLQVPMSEQLLPKNNGLIPQHKELETTCPIMASLEVRIQTFSPEPTQERLRLNTSEPLDGDMHAQVDEHKSERMLSPIPHNSAGSPIPPKQKADLTVTSASRTRSPSPQFFPQRLTDEPPANVYKKDPCSSKVEEPNTPGRKVPIQIVCTENGSERDIRNYLLHSESSEEPQTDQLKTLESSEQCHSPFIAYSNQIEEPILDPTEPMRSNGVSEHSEDDLKREELARDIMGKDRSLVDILDQRKMKTTMDLMEGIFPQGEQILEGAQQRRKAASKQTSPKNAQQRMEESLSSSVSLVSSSSYYSTSAPKAELLIKMKDMQEQDSEDELTTDLSSKKQELMDSLSKKLQVLREARESLQEDVQDNNALGEEVEATVQSVCRANELEKFRMFVGDLDKVVSLLLSLSGRLARVENALDNLEEGTSADEKQTLTEKRRLLIGQHEDAKELKENLDRRERVVYEILTGYFREEQLADYRHFVKMKSALIIEQRKLEDKIKLGEEQLKCLKESLPLEQRLLY

Target ORF information:

RefSeq Version	XM_679378.9
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio shroom family member 2b (shroom2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_679378.9

ORF Insert Sequence:

ATGGTGGATA TTGTAACGCA AACAATGCCT GCAGAAAGTG ACGTACACGT GGCCAGGAAC 
TTTCTCACCA AGATTTTGAG AAGTTCTATG AGGAGGAATG AATCGTCATG TAGACCCCAT 
TCATGGCACT CAGCTAAATC CTCGGAAAGC TCCAACCCAA ACGACTCCAA ATCTGAACCC 
AACCCAGCAC CTGACCCCAC TTGGCAGACA AAATGTGATC CTGGGTCACC CACAAAAGAA 
TTCACCAGCT GTTGGGGACA GACCAATCTG CGACAAGCAT CCTGCCAGTT CAGCTCGGTC 
AGAAATATGG AAAGCGTAGA GCCCTCATCT CATGCTTTCT CAAAAGAACA ACCTTTAACA 
AGCAAACCCA ATGGAGGAGC AGATCACGAG GTGCTTGAAA GTGCAATCAG CAAACCTGCT 
TTGCCAGGCA ATGCATTTTC AGGCACAGCT GATCATGGTG CACCTCGGGG TGCCAATCCC 
ACAGAAGGGG TGTTTTACAG AGGCGATCTA ACAGTCCCGA GTGACTGCCA CAGATACCTG 
CAGATCCCCA TAGGCAATGG GGGACGAGTG AGTCCCAGTG TTGAGGAGCA ATCTAGCTCC 
AGACTCTCCT CTACTGCGAG ATCAAACCAT AGACCTGTGT GGCATATTCC AGAGAATAAG 
ATGTCCAGTT CTGAAGCTCC TCCTGCTCCT CCTTTGCGGA GTGATAGTTT CACTGCCACC 
AGAGTTCATG AGAAGAGCTT AGTGGGATTG TTCCCTGAGG GACACACTGT ATATCCACTA 
CAGAGACCTC CCTGTAGAGC TGTGGACAGA CAATTAGAGG ACGTGAGACA CAGCTACAAC 
TCGCCTCCAA AGAAGGACTA CCTTGAGCCA TACCTTCCAG TTGAGGACTA CCCAAATCAG 
CTAAACCCTA CCAAACTCTT TTCCCTGTCA AGCACTGATG TAAGACAAGG CCAGAATCCG 
TTTGCCTCTG AGTTTCAGCA TCAGAGACAG CACAGTGATG ATAGTCCATT TTCTGTGTAT 
AACAACTCTA CATGTTCATC AAAGTCTCAG AGTGTTGGGA GCTACTACCG AAGTTTGCAA 
GATTTGCCTA ATAATGTTAG CAATCAAAAC AAAACTAGAA CCAGCACAGT CTTTGATCCC 
AACCATGATG GCCAAGCTCA TTTCCGTTAC TACTGCATCA CTGCCCAACA GCCATACCTG 
GAGTCATCTT CTCACTGTCG GATGGTAGAT GAACAAAGTT CTGAAATGGG AAAAGCACAA 
GGTGCCACAG ATAGAAGCTT TGTAGGTTCT CAAAAGGTTA TAAAAGCAAA GCATCCCCAA 
CTGTACCTTA GTTTACCCGA GAACAAGAGA TCCAATGGTT ACAACAAACA GTCCATCAGT 
CCAGCTTCAG CTCAAGCTGT GTCCCCGATA TCAAATAATT TGATTTCAAA GCACAGTTCT 
GTGGAAAGAG AGACTCAAAA AAAGAAAGAT AAGCCTCAGC CTTCCAGGCA CATTCCAAAC 
CACCAGACTG AGCAGAACTC CATGTACACT TACCAACATG ACAATCCATG GATCAGCAAG 
CAGGATCTGA AGATCTGTCC CCACAAAACC CCCTTGTTGC ATTCATTGGC CCAGGAGAGT 
AAGAAAATGG CAGAAAATTC GATTACAACA GCCTCCAAAG GAGGTAGTCA TGAATCAAGT 
GATGCCAGCA GTGGAAAGCT GGGAAGACGA AGTGACCGCT ATGCCACAAC TTTACGCAAA 
GAGATTCAAA AGAAGAGGGC TCAACTTCAA AAAAGCAGAA GCGCAGCTAC CTTGACTTGC 
TCAACTGAAG TTGAGGAGGA CTCTGACATA TGGAAGTCCA ATGAAACCTC CACATCCTCT 
TCTGATGGTT CCTTTACTAA CACGTACAAG GATCATCTGA AGGAAGCCCA AGCCAAAGTT 
CTACAGGCTA CATCATTCAT GAGGAAAGAT CTGGAGCTTC CTGGGAATGA AGTTGTGTCT 
GCTCAACTTC CCAAAAGACC TGGGCAAGTC ACCCGCATTG GAGGTCGTAA ACGCTTTCCC 
ATGGATAAAA AAATACATTC CTTCTCTGAA CCGGACAAGA TCAATGAGGT CGGAGTTGAA 
GATAAAGCTG GCGGGTCTTT TGCAGACCGA TACAAGTTCT TTGAAGGTTC TGGCAAAGCG 
GTTGTCCAAA AGCCCATTCC AAAGCAGTCC CTGCTTGGTC CTACTGTAGA CCACAGTGAG 
AGAAAGACTG GAGAAGGTGA AAGTATCCCT CGAGTACTGT TAAAGCAAAG TAAATCCAGC 
CATTCTGACC TTAATGCAGA TGAGCAACAA AGGCTTGGTA CATTTGCAGA ATATGAGGCT 
ACATGGAACT TGCAGAAGAA GCAAGTGGAA AGAAGAAACA CAGGCAGATA CCATTCAGCT 
GAAAATATTT TGGACTCAGG CTTGGAGGAA TCCAACAGTG CACTGTGTGT GCATGAAAGA 
TCACGCTCCT CACCTTCTGC TGACTTCTAT GGACAGAAAC TTCCTTTGCC ACCCAGAAAG 
TTACCTTTGG ATTCTCCACC TGAAGCCAAA GACCAACAGG AGGCTCATAT TTCCAGTGCA 
TCTGAAAAGG AACACAATGC ACTTAATATT TCCAAGTCTC AAGAGTCCAT CACAATGAGC 
ATTCTGGACA ATTCAGTTGA ACCTCTTGGA TACAACCAGA AATCCAGATC TGACACCAAG 
CAGCCAGCAC CGATTACTTC ACAACATCTC CAGGTTCCCA TGTCAGAGCA ACTTCTTCCC 
AAAAACAATG GTCTTATACC ACAACACAAA GAACTTGAAA CCACCTGCCC AATCATGGCT 
TCCCTAGAAG TCAGGATTCA GACCTTTTCA CCAGAGCCAA CACAAGAACG CCTGAGACTC 
AATACTTCAG AACCTCTGGA CGGAGACATG CATGCACAAG TGGATGAGCA TAAATCAGAG 
AGGATGTTAT CTCCTATTCC ACATAATTCA GCTGGTTCCC CTATCCCTCC CAAGCAGAAG 
GCTGACCTGA CGGTAACCTC AGCCAGCAGA ACACGTTCTC CTTCTCCTCA GTTCTTTCCA 
CAGAGACTCA CGGATGAACC TCCTGCAAAT GTGTACAAAA AGGATCCATG CAGTAGTAAG 
GTGGAAGAGC CAAACACTCC TGGCAGAAAG GTCCCCATAC AGATTGTTTG TACAGAAAAT 
GGTTCAGAGA GAGACATCCG AAACTATCTG CTGCACTCAG AATCATCTGA AGAGCCTCAA 
ACTGACCAGC TAAAAACCCT GGAATCTTCA GAGCAATGTC ATTCACCATT CATCGCATAC 
TCAAACCAGA TAGAAGAACC AATATTAGAC CCCACCGAAC CAATGAGGAG CAACGGTGTG 
AGCGAACACT CGGAGGACGA TCTGAAGCGA GAGGAGCTGG CCAGAGATAT AATGGGGAAA 
GACAGGAGTC TGGTGGACAT TCTGGACCAG AGAAAGATGA AGACCACCAT GGATCTGATG 
GAGGGAATAT TTCCACAGGG CGAGCAGATA CTGGAGGGAG CGCAGCAGAG GAGGAAAGCG 
GCGTCCAAAC AGACGTCTCC TAAAAATGCA CAGCAGAGGA TGGAGGAGAG TCTGTCTTCA 
TCAGTCTCGT TGGTCAGCAG TTCATCATAT TACAGCACAT CTGCACCCAA AGCAGAGCTG 
CTCATTAAGA TGAAGGACAT GCAGGAGCAG GACTCAGAGG ATGAGCTCAC TACTGACCTC 
TCCAGCAAGA AGCAAGAGCT GATGGACAGT TTGAGCAAGA AGCTGCAGGT TCTGAGAGAG 
GCTCGTGAAA GCCTGCAGGA GGACGTTCAG GATAATAACG CTCTGGGAGA GGAAGTGGAA 
GCCACAGTCC AGAGCGTCTG CAGAGCCAAT GAGCTGGAGA AGTTTCGCAT GTTTGTTGGA 
GATCTGGATA AAGTGGTCAG TCTGCTGCTG TCTCTGTCTG GACGGCTGGC ACGTGTGGAA 
AACGCACTCG ATAACCTGGA GGAGGGAACA TCCGCTGACG AGAAGCAAAC GTTGACGGAG 
AAGCGCAGGC TGCTGATTGG TCAACATGAA GACGCCAAAG AGCTGAAGGA GAATCTGGAC 
CGCCGCGAGC GCGTGGTTTA TGAGATCCTG ACGGGATACT TCAGGGAAGA GCAGCTGGCA 
GATTACAGAC ACTTCGTTAA AATGAAATCG GCGCTTATTA TAGAGCAGCG CAAACTGGAG 
GACAAGATAA AACTGGGAGA AGAGCAGCTG AAGTGTCTGA AGGAGAGTCT GCCGCTCGAA 
CAGAGACTCC TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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shroom2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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