shroom2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	shroom2
Entrez Gene ID	779568
Full Name	shroom family member 2
Synonyms	apxl,shrm2
General protein information	Preferred Names shroom family member 2 Names protein Shroom2 protein Apxl
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018091332.1	XP_017946821.1	protein Shroom2 isoform X3
NM_001078710.1	NP_001072178.1	protein Shroom2
XM_018091331.2	XP_017946820.1	protein Shroom2 isoform X1
XM_018091331.1	XP_017946820.1	protein Shroom2 isoform X1
XM_012957215.2	XP_012812669.1	protein Shroom2 isoform X2

Pathways from BioSystems

Homologies

	SHROOM2	XP_002806180.1
Rattus norvegicus (Norway rat)	Shroom2	NP_001041358.2
Gallus gallus (chicken)	SHROOM2	XP_416847.3
Homo sapiens (human)	SHROOM2	NP_001640.1
Canis lupus familiaris (dog)	SHROOM2	XP_005641152.1
Pan troglodytes (chimpanzee)	SHROOM2	XP_520924.4
Mus musculus (house mouse)	Shroom2	NP_766029.2
Danio rerio (zebrafish)	LOC556551	XP_005172998.1
Xenopus tropicalis (tropical clawed frog)	shroom2	NP_001072178.1

Gene Ontology
Bibliography

Related articles in PubMed

Shroom2 (APXL) regulates melanosome biogenesis and localization in the retinal pigment epithelium.
Fairbank PD, Lee C, Ellis A, Hildebrand JD, Gross JM, Wallingford JB
Development (Cambridge, England)133(20)4109-18(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following shroom2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shroom2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42597	XM_018091331.2 Latest version!	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa42598	XM_018091332.1 Latest version!	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa01957	NM_001078710.1 Latest version!	Xenopus tropicalis shroom family member 2 (shroom2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa28992	XM_012957215.2 Latest version!	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OXa42597	XM_018091331.1	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shroom2 ( XM_018091331.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946820.1 Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa42597
Clone ID Related Accession (Same CDS sequence)	XM_018091331.2 , XM_018091331.1
Accession Version	XM_018091331.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA GAGCTGGGCA GGAGAGGTTT CTGCCTGAGG ATACAAGGGT TTATTATGGT 
TTGTTGGATC AAGGGCACAC AGTGGTGGAG AGGAAACTAA TGGAAGGCTA CAGGCTGGTG 
GATGTTATTC TTACTGGAGG GGCTCCTTGG GGATTTACTT TGAAAGGTGG CAAAGAACAT 
GGAGAGCCTC TTATCATTAC CAAGGTGGAA GATGGAAACA AAGCTGCTGA CATATTAATG 
GCTGGAGATG AAATTGTCAA CATAAATGAT CAGCAGCTTT TAGGTTATCG TCAGGAGGCA 
ATATGTCTGG TTAAAGGATC ACACAAGATT CTAAAAATGA TAGTAAAACG CTCTTCATCT 
CATGATCTCT CAAATCCTTG GGACCAAACA AGCTTGCAAA GAAATTCTGG ACATTTTAGC 
TCCATGGGGA GTATGGACAG TATTGACCAA ACATATCAGT TTGGAAGACT TTCATCTGCT 
AAATCAAATA ACAGTATAGA TTATGTAGGA AGCCAGAATA AGAGAGACTC AGCATATGGT 
TCTTTTTCTG CAAGTTTCAG CACTCCAGAT CATACCTTAT CTAAAACTGC TTCTGCATCA 
ACAGAAAATA TTCTATATAA GAACAATGAG TGGGACAACA CAAAGCTAGG TTATGGCAAG 
ACAAGTCCAT CTATGAATGA AGTCAGACGT TCTGCAGATA GACAGGTATT ACAGTCTACT 
TCTATTAATG AGACAAGCAA AATACAGAGG ACAGAAGATA ACACTGAACC TAGATATTCT 
GGGCGATCAA ACTTTGGTCC TGTATGGAAT ATTCCTGATA AAAAAAAGAC AGCCTCACCT 
CCCCCTCCAC CACCACCACA GCGCAGTGAT AGTTATGCAG TCACTAAAAT CCATGAGAAG 
CCTACCAACC TTATGCACTT GGATGCTTCA AGCACTCAGC ACTTTAATGT TGCAAATAGG 
TCTCAAGCAA AACCTGACTG GAGCTTAGAA ATATCTGAAC AACAAAGACC TATAAGAGCA 
CATGACAGGA CTGTAGCTGA TACAAGAAGA ACCAGCAATT CCTCTTACCA TGCTGGCTTA 
AATGCAGATC AAGGGTTGTC CCCCTATAAG GACAAATATC CAAGTAATTT GCCCAATGTC 
AGTAGAATAC AGGCTTCATT ATCTACCAAT GATGTCAGGT TTGCACAACC TGCTTACAAC 
TATCATCACC AGCGGCAATA CAGTGATGAA AGCACTCTTT TTCAGAGCAC AAGAACATCA 
GCACAGCATA AATCCCAACA ACAGCCAATG AAATATGAAT CTAGTGTAAA TCAGGTGCCA 
TCTGATTTGA CTTATGTTTA CCATCCTCAT CAATTTAGAG CACCTGCTAC TTCTGCAGGT 
TTTAGTAGTG GGAAACAAAA TGTAGAAAAC AATGGGCAGA ACCATTTTCA CGTTGTGAGT 
ATAAAACATC CTCAGGGAAA TACAACACAC CATCAGTTCA AGGAGGAGGA AAACTATGCA 
CCAGAGGTGA ACTCTGGAAG AAAATCTGTC CAACCTGAAA ATACTGTCAT TAGCATTGAA 
ATACCAGCTT ACAGTCTACC TCAGGAAAGT TTTGAACCTT CACAAATAAA CTGTGAAAAG 
AACTACCTAA AAATATCTGA GAAAAAGGAT AATTATTTAG GTAATAATGA ACAAATAATC 
AATAAAACCA CTGGATATTC AGAAGAAAAA TGTAACGACA GATTTAGCCA GCTGGACCAT 
TCTGAAAAAG GTTCATATAG ATCAAGTCAA GATTATTCTT GGAGAAAGGA AGAAAATAAG 
ATAACACCTC TAGTAACACC AATGTTGCAT TCTTTAGCTC AAGAAGGAAG AAATCGATCT 
GAAAGTTTCC CAGATACTGG AAATGAAAAA CTGTCTTTTC CAGATGCGGG CAAACAGTCT 
CGTAGAAGTG ATCGGTTTGC AACGACTCTG AGAAATGAAA TTCAGCAGAG GCGAGCTCGT 
CTGCAAAAGA GCAAAAGTAC AGCTGCCTTG ACCGAGTCAA ACGAAACAGA GACCTCTGAC 
AACTGGAAAC AAGACTCACT GGAAAGCATG AGCCCAACTT CTGAAGGTTC TTTTTCAAGC 
ACCTACAGAA GTCATCTGCA AGAAGCCCAG GCCCGTGTTT TAAGAGCTAC TTCCTTTAAA 
AGAAGAGACC TTGATACAGG TATTTCTGAT CATCTTTCAC TGTTTCAAGA TCGAAATATG 
CAGATCTCAA GCTTTTCAGG CCTATCTTTA GATCCTGTTC AACCTAAAAA AAACACAGCT 
GTTAACAGCT CACAAAATGT ATCTCGTATT GGAGCAAGAA AGAGGTTCAC AACTCAGCAG 
AAGCTGATGT CCTACTCAGA GCCTGAGAAA ATTAATGAAG TTGGTGTTGA AGACCAATAC 
AATTTCCGTA CTGAAAATTC TACTAAAAGA ACTGTGGGAT CTTTTGCAGA CAGATGGAAA 
TTCTTTGAAG AGACCAGCAA ATGTGCACAA CCAAAGGTTC CACCAAAAGT TGTTTCTTCT 
AGCCAGTCAG AAGAAACTTC TGAAATAGCC ATTAACAGGG ATTATGCCAA GAGTTCTGAG 
GGGCAAGAGA GCAAAAGGGC TTTGGCAGTG TCTGGACAAA ATCCTGCTGA TGAAAACGGC 
TTCCCTGATA AAGTTACAAC AGAACGTCGT CAAAGATTAG GGACTTTTGC AGAATATGAG 
GCATCTTGGA AAGAACAGAA AACACAGCTG GAGAGAAAAA ATTCTGGCAG ATGTCATTCG 
GCTGACAACA TCCTTGATGC TGATCTTGAG CAGAATCCTA AAGCTCAGTA TATTCATGAG 
CGGTCCAAGT CTTCTCCTAC CACAGATTTT TATGCACAGG CAGCAGCCGT TGAGTCAAAG 
CAGCAGTCAG AGAGCGTGAG GAGAGACACC GAGAATTCTA ACTCGACACA CTGCCGCAGT 
AGCACAGGGG ATTCTCCCAC AAGAACTGTT GAAGCTGGCG ATCAGTGTGG TGCTACCAAT 
GAACAAGAGG TTTTAACCTG CAATACTAAA TGGAAGCCAC ATGATAAATC GTTTCCGATC 
CCAGAAACAT CCAATGAATC CCAGCAGAGC AGAGCAAGAT CAGGGACGCT ACCAAATGAT 
TATAGATTTG CTCATGAAAA TGTGAATCAA GGAAATCGGG ATATCAGCTT TTCTGCTGTT 
CCTATATCTG AAGCCTGCCC TGATTTCTCC AATGCAGACA GCGATCAGTT GCAAGATCAC 
CCATCTGTGT TTAAGAAGCG CAGTGCTGCC CCACAAAGGC CTCCTCCGCC TAAACTTGAA 
AACAAATATT GGCGGCAGAA TGGCTCCTCA TCTTCCCTTG CCACATCCTC AGAGTCATTG 
CTGACAGCCC AAGCCAGGAG GGCACAATCC TATTCCCCTA GCAGCCAAGA CACATTTCCT 
CCTCAGTCTC TTCAGAAACA AAGCCCAAGT ACATATCCTG ATAAGAATCC TAGCATTCAT 
ATATATGATT ATCAATTATC TGTGCCACCA GAGAATGACA GGTATCATCT AGAAAAGAAA 
TACTTTGAAT CTGAACTGTC CTCAAAGTCA CACTTGCAGA TTCCTGGAAT GGAACCGTCT 
CGATCACCCT CTCCTCAGTT TGCTCCACAG AAGCTTACAG ACAAGCCTCC CCTTCTGGTT 
CCAGAAGAAA ACTTGTCAAG GATTGAAAGG GTGATCGATA ACACTACAGT GAAAATGGTC 
CCCATAAAGA TTGTTCACTC TGAAACTCAT GCTGAGAAGG AAAGCAGACA CAATTTGCTG 
AGTGCTATTG AGCCCACTGC CTTACCAACT GGTCTTGCCA AGGACCAACT AAAGACTTTG 
AGCACATCAG AACAATCCTA CTCCCGATTT TGTGCTTACA CCAGGCAGGA ATCTAGAGAG 
GAAGAGGAAT CCAGAGTGAC TGACCTTTAC TCATGCCAGA GAAATGCAGA AGATGATAAT 
GAAAATGATG TATCTTCTCT TGCACCCTCA AATGCTAAAA GCAAAGATGC AATCTACGCG 
GATTTAAAGT CAGAGGAGCT GGTGAGGGAA ATTGTAGATA AAGATAAATC TCTTGCGGAC 
ATTTTAGATC CAAATGCCAA GATGAGAACT ACCATGGACT TAATGGAGGG AATCTTTCCA 
AAAGATGAAC ATCTTCTAGA AGAAGCTCAG CAGCGTAGAA AACATCTCCC AAAAATACCA 
TCCCCAAGAT CAACAGATGA CAAAAAAGAT GAGCAAAATG TCCCATCAGC TGTTTCTCTG 
ACAACCAGTT CCACGTATTA CAGCACTTCA GCAGCCAAAG CCGAGCTCCT TATTAAAATG 
AAAGACATGC AGGAGCAGCA GCACATTGCG GAGAATTCAG AGGATGAGCT GGGTCAGAAC 
CTATCCGAGA AAAAGCAAGA ACTGATTGAC AGCATTAGCA AGAAGCTGCA GGTCCTGCGA 
GATGCAAAGG AAACCCTGTT AGAAGATGTC CAGTGTAACA ATGCTCTGGG GGAAGAGGTA 
GAAATCATTG TGAAAGAAGT CTGCAAACCA AATGAATTTG ACAAATTCAG GATGTTCATT 
GGGGATCTGG AGAAAATCGT CAATCTTCTG CTGTCACTTT CTGGGCGACT TGCTCGAGTG 
GAAAATGCTC TTAATAACTT GGATGAAACC GTGTCTCCTG AAGAGCGGAA AACACTGTTG 
GAAAAAAGGA AATTACTTAC GAGACAGCAT GAAGACGCAA AGGAACTGAA GGAGAACCTA 
GACCGGCGTG AGCGCACCGT TTATGAGATT TTGGCTAATT ACCTTAATGA GGAAAACCTT 
GCAGATTATG AGCACTTTGT GAAGATGAAG TCGGCACTCA TCCTTGAGCA GCGAGAGCTG 
GAAGACAAAA TCAAGCTTGG AGAGGAGCAG CTCAAATGCC TGACCGATAG TCTTCCATTG 
GATCGTATCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946820.1
CDS	940..5874
Translation	MEPRAGQERFLPEDTRVYYGLLDQGHTVVERKLMEGYRLVDVILTGGAPWGFTLKGGKEHGEPLIITKVEDGNKAADILMAGDEIVNINDQQLLGYRQEAICLVKGSHKILKMIVKRSSSHDLSNPWDQTSLQRNSGHFSSMGSMDSIDQTYQFGRLSSAKSNNSIDYVGSQNKRDSAYGSFSASFSTPDHTLSKTASASTENILYKNNEWDNTKLGYGKTSPSMNEVRRSADRQVLQSTSINETSKIQRTEDNTEPRYSGRSNFGPVWNIPDKKKTASPPPPPPPQRSDSYAVTKIHEKPTNLMHLDASSTQHFNVANRSQAKPDWSLEISEQQRPIRAHDRTVADTRRTSNSSYHAGLNADQGLSPYKDKYPSNLPNVSRIQASLSTNDVRFAQPAYNYHHQRQYSDESTLFQSTRTSAQHKSQQQPMKYESSVNQVPSDLTYVYHPHQFRAPATSAGFSSGKQNVENNGQNHFHVVSIKHPQGNTTHHQFKEEENYAPEVNSGRKSVQPENTVISIEIPAYSLPQESFEPSQINCEKNYLKISEKKDNYLGNNEQIINKTTGYSEEKCNDRFSQLDHSEKGSYRSSQDYSWRKEENKITPLVTPMLHSLAQEGRNRSESFPDTGNEKLSFPDAGKQSRRSDRFATTLRNEIQQRRARLQKSKSTAALTESNETETSDNWKQDSLESMSPTSEGSFSSTYRSHLQEAQARVLRATSFKRRDLDTGISDHLSLFQDRNMQISSFSGLSLDPVQPKKNTAVNSSQNVSRIGARKRFTTQQKLMSYSEPEKINEVGVEDQYNFRTENSTKRTVGSFADRWKFFEETSKCAQPKVPPKVVSSSQSEETSEIAINRDYAKSSEGQESKRALAVSGQNPADENGFPDKVTTERRQRLGTFAEYEASWKEQKTQLERKNSGRCHSADNILDADLEQNPKAQYIHERSKSSPTTDFYAQAAAVESKQQSESVRRDTENSNSTHCRSSTGDSPTRTVEAGDQCGATNEQEVLTCNTKWKPHDKSFPIPETSNESQQSRARSGTLPNDYRFAHENVNQGNRDISFSAVPISEACPDFSNADSDQLQDHPSVFKKRSAAPQRPPPPKLENKYWRQNGSSSSLATSSESLLTAQARRAQSYSPSSQDTFPPQSLQKQSPSTYPDKNPSIHIYDYQLSVPPENDRYHLEKKYFESELSSKSHLQIPGMEPSRSPSPQFAPQKLTDKPPLLVPEENLSRIERVIDNTTVKMVPIKIVHSETHAEKESRHNLLSAIEPTALPTGLAKDQLKTLSTSEQSYSRFCAYTRQESREEEESRVTDLYSCQRNAEDDNENDVSSLAPSNAKSKDAIYADLKSEELVREIVDKDKSLADILDPNAKMRTTMDLMEGIFPKDEHLLEEAQQRRKHLPKIPSPRSTDDKKDEQNVPSAVSLTTSSTYYSTSAAKAELLIKMKDMQEQQHIAENSEDELGQNLSEKKQELIDSISKKLQVLRDAKETLLEDVQCNNALGEEVEIIVKEVCKPNEFDKFRMFIGDLEKIVNLLLSLSGRLARVENALNNLDETVSPEERKTLLEKRKLLTRQHEDAKELKENLDRRERTVYEILANYLNEENLADYEHFVKMKSALILEQRELEDKIKLGEEQLKCLTDSLPLDRIK

Target ORF information:

RefSeq Version	XM_018091331.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091331.2

ORF Insert Sequence:

ATGGAGCCCA GAGCTGGGCA GGAGAGGTTT CTGCCTGAGG ATACAAGGGT TTATTATGGT 
TTGTTGGATC AAGGGCACAC AGTGGTGGAG AGGAAACTAA TGGAAGGCTA CAGGCTGGTG 
GATGTTATTC TTACTGGAGG GGCTCCTTGG GGATTTACTT TGAAAGGTGG CAAAGAACAT 
GGAGAGCCTC TTATCATTAC CAAGGTGGAA GATGGAAACA AAGCTGCTGA CATATTAATG 
GCTGGAGATG AAATTGTCAA CATAAATGAT CAGCAGCTTT TAGGTTATCG TCAGGAGGCA 
ATATGTCTGG TTAAAGGATC ACACAAGATT CTAAAAATGA TAGTAAAACG CTCTTCATCT 
CATGATCTCT CAAATCCTTG GGACCAAACA AGCTTGCAAA GAAATTCTGG ACATTTTAGC 
TCCATGGGGA GTATGGACAG TATTGACCAA ACATATCAGT TTGGAAGACT TTCATCTGCT 
AAATCAAATA ACAGTATAGA TTATGTAGGA AGCCAGAATA AGAGAGACTC AGCATATGGT 
TCTTTTTCTG CAAGTTTCAG CACTCCAGAT CATACCTTAT CTAAAACTGC TTCTGCATCA 
ACAGAAAATA TTCTATATAA GAACAATGAG TGGGACAACA CAAAGCTAGG TTATGGCAAG 
ACAAGTCCAT CTATGAATGA AGTCAGACGT TCTGCAGATA GACAGGTATT ACAGTCTACT 
TCTATTAATG AGACAAGCAA AATACAGAGG ACAGAAGATA ACACTGAACC TAGATATTCT 
GGGCGATCAA ACTTTGGTCC TGTATGGAAT ATTCCTGATA AAAAAAAGAC AGCCTCACCT 
CCCCCTCCAC CACCACCACA GCGCAGTGAT AGTTATGCAG TCACTAAAAT CCATGAGAAG 
CCTACCAACC TTATGCACTT GGATGCTTCA AGCACTCAGC ACTTTAATGT TGCAAATAGG 
TCTCAAGCAA AACCTGACTG GAGCTTAGAA ATATCTGAAC AACAAAGACC TATAAGAGCA 
CATGACAGGA CTGTAGCTGA TACAAGAAGA ACCAGCAATT CCTCTTACCA TGCTGGCTTA 
AATGCAGATC AAGGGTTGTC CCCCTATAAG GACAAATATC CAAGTAATTT GCCCAATGTC 
AGTAGAATAC AGGCTTCATT ATCTACCAAT GATGTCAGGT TTGCACAACC TGCTTACAAC 
TATCATCACC AGCGGCAATA CAGTGATGAA AGCACTCTTT TTCAGAGCAC AAGAACATCA 
GCACAGCATA AATCCCAACA ACAGCCAATG AAATATGAAT CTAGTGTAAA TCAGGTGCCA 
TCTGATTTGA CTTATGTTTA CCATCCTCAT CAATTTAGAG CACCTGCTAC TTCTGCAGGT 
TTTAGTAGTG GGAAACAAAA TGTAGAAAAC AATGGGCAGA ACCATTTTCA CGTTGTGAGT 
ATAAAACATC CTCAGGGAAA TACAACACAC CATCAGTTCA AGGAGGAGGA AAACTATGCA 
CCAGAGGTGA ACTCTGGAAG AAAATCTGTC CAACCTGAAA ATACTGTCAT TAGCATTGAA 
ATACCAGCTT ACAGTCTACC TCAGGAAAGT TTTGAACCTT CACAAATAAA CTGTGAAAAG 
AACTACCTAA AAATATCTGA GAAAAAGGAT AATTATTTAG GTAATAATGA ACAAATAATC 
AATAAAACCA CTGGATATTC AGAAGAAAAA TGTAACGACA GATTTAGCCA GCTGGACCAT 
TCTGAAAAAG GTTCATATAG ATCAAGTCAA GATTATTCTT GGAGAAAGGA AGAAAATAAG 
ATAACACCTC TAGTAACACC AATGTTGCAT TCTTTAGCTC AAGAAGGAAG AAATCGATCT 
GAAAGTTTCC CAGATACTGG AAATGAAAAA CTGTCTTTTC CAGATGCGGG CAAACAGTCT 
CGTAGAAGTG ATCGGTTTGC AACGACTCTG AGAAATGAAA TTCAGCAGAG GCGAGCTCGT 
CTGCAAAAGA GCAAAAGTAC AGCTGCCTTG ACCGAGTCAA ACGAAACAGA GACCTCTGAC 
AACTGGAAAC AAGACTCACT GGAAAGCATG AGCCCAACTT CTGAAGGTTC TTTTTCAAGC 
ACCTACAGAA GTCATCTGCA AGAAGCCCAG GCCCGTGTTT TAAGAGCTAC TTCCTTTAAA 
AGAAGAGACC TTGATACAGG TATTTCTGAT CATCTTTCAC TGTTTCAAGA TCGAAATATG 
CAGATCTCAA GCTTTTCAGG CCTATCTTTA GATCCTGTTC AACCTAAAAA AAACACAGCT 
GTTAACAGCT CACAAAATGT ATCTCGTATT GGAGCAAGAA AGAGGTTCAC AACTCAGCAG 
AAGCTGATGT CCTACTCAGA GCCTGAGAAA ATTAATGAAG TTGGTGTTGA AGACCAATAC 
AATTTCCGTA CTGAAAATTC TACTAAAAGA ACTGTGGGAT CTTTTGCAGA CAGATGGAAA 
TTCTTTGAAG AGACCAGCAA ATGTGCACAA CCAAAGGTTC CACCAAAAGT TGTTTCTTCT 
AGCCAGTCAG AAGAAACTTC TGAAATAGCC ATTAACAGGG ATTATGCCAA GAGTTCTGAG 
GGGCAAGAGA GCAAAAGGGC TTTGGCAGTG TCTGGACAAA ATCCTGCTGA TGAAAACGGC 
TTCCCTGATA AAGTTACAAC AGAACGTCGT CAAAGATTAG GGACTTTTGC AGAATATGAG 
GCATCTTGGA AAGAACAGAA AACACAGCTG GAGAGAAAAA ATTCTGGCAG ATGTCATTCG 
GCTGACAACA TCCTTGATGC TGATCTTGAG CAGAATCCTA AAGCTCAGTA TATTCATGAG 
CGGTCCAAGT CTTCTCCTAC CACAGATTTT TATGCACAGG CAGCAGCCGT TGAGTCAAAG 
CAGCAGTCAG AGAGCGTGAG GAGAGACACC GAGAATTCTA ACTCGACACA CTGCCGCAGT 
AGCACAGGGG ATTCTCCCAC AAGAACTGTT GAAGCTGGCG ATCAGTGTGG TGCTACCAAT 
GAACAAGAGG TTTTAACCTG CAATACTAAA TGGAAGCCAC ATGATAAATC GTTTCCGATC 
CCAGAAACAT CCAATGAATC CCAGCAGAGC AGAGCAAGAT CAGGGACGCT ACCAAATGAT 
TATAGATTTG CTCATGAAAA TGTGAATCAA GGAAATCGGG ATATCAGCTT TTCTGCTGTT 
CCTATATCTG AAGCCTGCCC TGATTTCTCC AATGCAGACA GCGATCAGTT GCAAGATCAC 
CCATCTGTGT TTAAGAAGCG CAGTGCTGCC CCACAAAGGC CTCCTCCGCC TAAACTTGAA 
AACAAATATT GGCGGCAGAA TGGCTCCTCA TCTTCCCTTG CCACATCCTC AGAGTCATTG 
CTGACAGCCC AAGCCAGGAG GGCACAATCC TATTCCCCTA GCAGCCAAGA CACATTTCCT 
CCTCAGTCTC TTCAGAAACA AAGCCCAAGT ACATATCCTG ATAAGAATCC TAGCATTCAT 
ATATATGATT ATCAATTATC TGTGCCACCA GAGAATGACA GGTATCATCT AGAAAAGAAA 
TACTTTGAAT CTGAACTGTC CTCAAAGTCA CACTTGCAGA TTCCTGGAAT GGAACCGTCT 
CGATCACCCT CTCCTCAGTT TGCTCCACAG AAGCTTACAG ACAAGCCTCC CCTTCTGGTT 
CCAGAAGAAA ACTTGTCAAG GATTGAAAGG GTGATCGATA ACACTACAGT GAAAATGGTC 
CCCATAAAGA TTGTTCACTC TGAAACTCAT GCTGAGAAGG AAAGCAGACA CAATTTGCTG 
AGTGCTATTG AGCCCACTGC CTTACCAACT GGTCTTGCCA AGGACCAACT AAAGACTTTG 
AGCACATCAG AACAATCCTA CTCCCGATTT TGTGCTTACA CCAGGCAGGA ATCTAGAGAG 
GAAGAGGAAT CCAGAGTGAC TGACCTTTAC TCATGCCAGA GAAATGCAGA AGATGATAAT 
GAAAATGATG TATCTTCTCT TGCACCCTCA AATGCTAAAA GCAAAGATGC AATCTACGCG 
GATTTAAAGT CAGAGGAGCT GGTGAGGGAA ATTGTAGATA AAGATAAATC TCTTGCGGAC 
ATTTTAGATC CAAATGCCAA GATGAGAACT ACCATGGACT TAATGGAGGG AATCTTTCCA 
AAAGATGAAC ATCTTCTAGA AGAAGCTCAG CAGCGTAGAA AACATCTCCC AAAAATACCA 
TCCCCAAGAT CAACAGATGA CAAAAAAGAT GAGCAAAATG TCCCATCAGC TGTTTCTCTG 
ACAACCAGTT CCACGTATTA CAGCACTTCA GCAGCCAAAG CCGAGCTCCT TATTAAAATG 
AAAGACATGC AGGAGCAGCA GCACATTGCG GAGAATTCAG AGGATGAGCT GGGTCAGAAC 
CTATCCGAGA AAAAGCAAGA ACTGATTGAC AGCATTAGCA AGAAGCTGCA GGTCCTGCGA 
GATGCAAAGG AAACCCTGTT AGAAGATGTC CAGTGTAACA ATGCTCTGGG GGAAGAGGTA 
GAAATCATTG TGAAAGAAGT CTGCAAACCA AATGAATTTG ACAAATTCAG GATGTTCATT 
GGGGATCTGG AGAAAATCGT CAATCTTCTG CTGTCACTTT CTGGGCGACT TGCTCGAGTG 
GAAAATGCTC TTAATAACTT GGATGAAACC GTGTCTCCTG AAGAGCGGAA AACACTGTTG 
GAAAAAAGGA AATTACTTAC GAGACAGCAT GAAGACGCAA AGGAACTGAA GGAGAACCTA 
GACCGGCGTG AGCGCACCGT TTATGAGATT TTGGCTAATT ACCTTAATGA GGAAAACCTT 
GCAGATTATG AGCACTTTGT GAAGATGAAG TCGGCACTCA TCCTTGAGCA GCGAGAGCTG 
GAAGACAAAA TCAAGCTTGG AGAGGAGCAG CTCAAATGCC TGACCGATAG TCTTCCATTG 
GATCGTATCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2 ( XM_018091331.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946820.1 Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa42597
Clone ID Related Accession (Same CDS sequence)	XM_018091331.2 , XM_018091331.1
Accession Version	XM_018091331.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA GAGCTGGGCA GGAGAGGTTT CTGCCTGAGG ATACAAGGGT TTATTATGGT 
TTGTTGGATC AAGGGCACAC AGTGGTGGAG AGGAAACTAA TGGAAGGCTA CAGGCTGGTG 
GATGTTATTC TTACTGGAGG GGCTCCTTGG GGATTTACTT TGAAAGGTGG CAAAGAACAT 
GGAGAGCCTC TTATCATTAC CAAGGTGGAA GATGGAAACA AAGCTGCTGA CATATTAATG 
GCTGGAGATG AAATTGTCAA CATAAATGAT CAGCAGCTTT TAGGTTATCG TCAGGAGGCA 
ATATGTCTGG TTAAAGGATC ACACAAGATT CTAAAAATGA TAGTAAAACG CTCTTCATCT 
CATGATCTCT CAAATCCTTG GGACCAAACA AGCTTGCAAA GAAATTCTGG ACATTTTAGC 
TCCATGGGGA GTATGGACAG TATTGACCAA ACATATCAGT TTGGAAGACT TTCATCTGCT 
AAATCAAATA ACAGTATAGA TTATGTAGGA AGCCAGAATA AGAGAGACTC AGCATATGGT 
TCTTTTTCTG CAAGTTTCAG CACTCCAGAT CATACCTTAT CTAAAACTGC TTCTGCATCA 
ACAGAAAATA TTCTATATAA GAACAATGAG TGGGACAACA CAAAGCTAGG TTATGGCAAG 
ACAAGTCCAT CTATGAATGA AGTCAGACGT TCTGCAGATA GACAGGTATT ACAGTCTACT 
TCTATTAATG AGACAAGCAA AATACAGAGG ACAGAAGATA ACACTGAACC TAGATATTCT 
GGGCGATCAA ACTTTGGTCC TGTATGGAAT ATTCCTGATA AAAAAAAGAC AGCCTCACCT 
CCCCCTCCAC CACCACCACA GCGCAGTGAT AGTTATGCAG TCACTAAAAT CCATGAGAAG 
CCTACCAACC TTATGCACTT GGATGCTTCA AGCACTCAGC ACTTTAATGT TGCAAATAGG 
TCTCAAGCAA AACCTGACTG GAGCTTAGAA ATATCTGAAC AACAAAGACC TATAAGAGCA 
CATGACAGGA CTGTAGCTGA TACAAGAAGA ACCAGCAATT CCTCTTACCA TGCTGGCTTA 
AATGCAGATC AAGGGTTGTC CCCCTATAAG GACAAATATC CAAGTAATTT GCCCAATGTC 
AGTAGAATAC AGGCTTCATT ATCTACCAAT GATGTCAGGT TTGCACAACC TGCTTACAAC 
TATCATCACC AGCGGCAATA CAGTGATGAA AGCACTCTTT TTCAGAGCAC AAGAACATCA 
GCACAGCATA AATCCCAACA ACAGCCAATG AAATATGAAT CTAGTGTAAA TCAGGTGCCA 
TCTGATTTGA CTTATGTTTA CCATCCTCAT CAATTTAGAG CACCTGCTAC TTCTGCAGGT 
TTTAGTAGTG GGAAACAAAA TGTAGAAAAC AATGGGCAGA ACCATTTTCA CGTTGTGAGT 
ATAAAACATC CTCAGGGAAA TACAACACAC CATCAGTTCA AGGAGGAGGA AAACTATGCA 
CCAGAGGTGA ACTCTGGAAG AAAATCTGTC CAACCTGAAA ATACTGTCAT TAGCATTGAA 
ATACCAGCTT ACAGTCTACC TCAGGAAAGT TTTGAACCTT CACAAATAAA CTGTGAAAAG 
AACTACCTAA AAATATCTGA GAAAAAGGAT AATTATTTAG GTAATAATGA ACAAATAATC 
AATAAAACCA CTGGATATTC AGAAGAAAAA TGTAACGACA GATTTAGCCA GCTGGACCAT 
TCTGAAAAAG GTTCATATAG ATCAAGTCAA GATTATTCTT GGAGAAAGGA AGAAAATAAG 
ATAACACCTC TAGTAACACC AATGTTGCAT TCTTTAGCTC AAGAAGGAAG AAATCGATCT 
GAAAGTTTCC CAGATACTGG AAATGAAAAA CTGTCTTTTC CAGATGCGGG CAAACAGTCT 
CGTAGAAGTG ATCGGTTTGC AACGACTCTG AGAAATGAAA TTCAGCAGAG GCGAGCTCGT 
CTGCAAAAGA GCAAAAGTAC AGCTGCCTTG ACCGAGTCAA ACGAAACAGA GACCTCTGAC 
AACTGGAAAC AAGACTCACT GGAAAGCATG AGCCCAACTT CTGAAGGTTC TTTTTCAAGC 
ACCTACAGAA GTCATCTGCA AGAAGCCCAG GCCCGTGTTT TAAGAGCTAC TTCCTTTAAA 
AGAAGAGACC TTGATACAGG TATTTCTGAT CATCTTTCAC TGTTTCAAGA TCGAAATATG 
CAGATCTCAA GCTTTTCAGG CCTATCTTTA GATCCTGTTC AACCTAAAAA AAACACAGCT 
GTTAACAGCT CACAAAATGT ATCTCGTATT GGAGCAAGAA AGAGGTTCAC AACTCAGCAG 
AAGCTGATGT CCTACTCAGA GCCTGAGAAA ATTAATGAAG TTGGTGTTGA AGACCAATAC 
AATTTCCGTA CTGAAAATTC TACTAAAAGA ACTGTGGGAT CTTTTGCAGA CAGATGGAAA 
TTCTTTGAAG AGACCAGCAA ATGTGCACAA CCAAAGGTTC CACCAAAAGT TGTTTCTTCT 
AGCCAGTCAG AAGAAACTTC TGAAATAGCC ATTAACAGGG ATTATGCCAA GAGTTCTGAG 
GGGCAAGAGA GCAAAAGGGC TTTGGCAGTG TCTGGACAAA ATCCTGCTGA TGAAAACGGC 
TTCCCTGATA AAGTTACAAC AGAACGTCGT CAAAGATTAG GGACTTTTGC AGAATATGAG 
GCATCTTGGA AAGAACAGAA AACACAGCTG GAGAGAAAAA ATTCTGGCAG ATGTCATTCG 
GCTGACAACA TCCTTGATGC TGATCTTGAG CAGAATCCTA AAGCTCAGTA TATTCATGAG 
CGGTCCAAGT CTTCTCCTAC CACAGATTTT TATGCACAGG CAGCAGCCGT TGAGTCAAAG 
CAGCAGTCAG AGAGCGTGAG GAGAGACACC GAGAATTCTA ACTCGACACA CTGCCGCAGT 
AGCACAGGGG ATTCTCCCAC AAGAACTGTT GAAGCTGGCG ATCAGTGTGG TGCTACCAAT 
GAACAAGAGG TTTTAACCTG CAATACTAAA TGGAAGCCAC ATGATAAATC GTTTCCGATC 
CCAGAAACAT CCAATGAATC CCAGCAGAGC AGAGCAAGAT CAGGGACGCT ACCAAATGAT 
TATAGATTTG CTCATGAAAA TGTGAATCAA GGAAATCGGG ATATCAGCTT TTCTGCTGTT 
CCTATATCTG AAGCCTGCCC TGATTTCTCC AATGCAGACA GCGATCAGTT GCAAGATCAC 
CCATCTGTGT TTAAGAAGCG CAGTGCTGCC CCACAAAGGC CTCCTCCGCC TAAACTTGAA 
AACAAATATT GGCGGCAGAA TGGCTCCTCA TCTTCCCTTG CCACATCCTC AGAGTCATTG 
CTGACAGCCC AAGCCAGGAG GGCACAATCC TATTCCCCTA GCAGCCAAGA CACATTTCCT 
CCTCAGTCTC TTCAGAAACA AAGCCCAAGT ACATATCCTG ATAAGAATCC TAGCATTCAT 
ATATATGATT ATCAATTATC TGTGCCACCA GAGAATGACA GGTATCATCT AGAAAAGAAA 
TACTTTGAAT CTGAACTGTC CTCAAAGTCA CACTTGCAGA TTCCTGGAAT GGAACCGTCT 
CGATCACCCT CTCCTCAGTT TGCTCCACAG AAGCTTACAG ACAAGCCTCC CCTTCTGGTT 
CCAGAAGAAA ACTTGTCAAG GATTGAAAGG GTGATCGATA ACACTACAGT GAAAATGGTC 
CCCATAAAGA TTGTTCACTC TGAAACTCAT GCTGAGAAGG AAAGCAGACA CAATTTGCTG 
AGTGCTATTG AGCCCACTGC CTTACCAACT GGTCTTGCCA AGGACCAACT AAAGACTTTG 
AGCACATCAG AACAATCCTA CTCCCGATTT TGTGCTTACA CCAGGCAGGA ATCTAGAGAG 
GAAGAGGAAT CCAGAGTGAC TGACCTTTAC TCATGCCAGA GAAATGCAGA AGATGATAAT 
GAAAATGATG TATCTTCTCT TGCACCCTCA AATGCTAAAA GCAAAGATGC AATCTACGCG 
GATTTAAAGT CAGAGGAGCT GGTGAGGGAA ATTGTAGATA AAGATAAATC TCTTGCGGAC 
ATTTTAGATC CAAATGCCAA GATGAGAACT ACCATGGACT TAATGGAGGG AATCTTTCCA 
AAAGATGAAC ATCTTCTAGA AGAAGCTCAG CAGCGTAGAA AACATCTCCC AAAAATACCA 
TCCCCAAGAT CAACAGATGA CAAAAAAGAT GAGCAAAATG TCCCATCAGC TGTTTCTCTG 
ACAACCAGTT CCACGTATTA CAGCACTTCA GCAGCCAAAG CCGAGCTCCT TATTAAAATG 
AAAGACATGC AGGAGCAGCA GCACATTGCG GAGAATTCAG AGGATGAGCT GGGTCAGAAC 
CTATCCGAGA AAAAGCAAGA ACTGATTGAC AGCATTAGCA AGAAGCTGCA GGTCCTGCGA 
GATGCAAAGG AAACCCTGTT AGAAGATGTC CAGTGTAACA ATGCTCTGGG GGAAGAGGTA 
GAAATCATTG TGAAAGAAGT CTGCAAACCA AATGAATTTG ACAAATTCAG GATGTTCATT 
GGGGATCTGG AGAAAATCGT CAATCTTCTG CTGTCACTTT CTGGGCGACT TGCTCGAGTG 
GAAAATGCTC TTAATAACTT GGATGAAACC GTGTCTCCTG AAGAGCGGAA AACACTGTTG 
GAAAAAAGGA AATTACTTAC GAGACAGCAT GAAGACGCAA AGGAACTGAA GGAGAACCTA 
GACCGGCGTG AGCGCACCGT TTATGAGATT TTGGCTAATT ACCTTAATGA GGAAAACCTT 
GCAGATTATG AGCACTTTGT GAAGATGAAG TCGGCACTCA TCCTTGAGCA GCGAGAGCTG 
GAAGACAAAA TCAAGCTTGG AGAGGAGCAG CTCAAATGCC TGACCGATAG TCTTCCATTG 
GATCGTATCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946820.1
CDS	933..5867
Translation	MEPRAGQERFLPEDTRVYYGLLDQGHTVVERKLMEGYRLVDVILTGGAPWGFTLKGGKEHGEPLIITKVEDGNKAADILMAGDEIVNINDQQLLGYRQEAICLVKGSHKILKMIVKRSSSHDLSNPWDQTSLQRNSGHFSSMGSMDSIDQTYQFGRLSSAKSNNSIDYVGSQNKRDSAYGSFSASFSTPDHTLSKTASASTENILYKNNEWDNTKLGYGKTSPSMNEVRRSADRQVLQSTSINETSKIQRTEDNTEPRYSGRSNFGPVWNIPDKKKTASPPPPPPPQRSDSYAVTKIHEKPTNLMHLDASSTQHFNVANRSQAKPDWSLEISEQQRPIRAHDRTVADTRRTSNSSYHAGLNADQGLSPYKDKYPSNLPNVSRIQASLSTNDVRFAQPAYNYHHQRQYSDESTLFQSTRTSAQHKSQQQPMKYESSVNQVPSDLTYVYHPHQFRAPATSAGFSSGKQNVENNGQNHFHVVSIKHPQGNTTHHQFKEEENYAPEVNSGRKSVQPENTVISIEIPAYSLPQESFEPSQINCEKNYLKISEKKDNYLGNNEQIINKTTGYSEEKCNDRFSQLDHSEKGSYRSSQDYSWRKEENKITPLVTPMLHSLAQEGRNRSESFPDTGNEKLSFPDAGKQSRRSDRFATTLRNEIQQRRARLQKSKSTAALTESNETETSDNWKQDSLESMSPTSEGSFSSTYRSHLQEAQARVLRATSFKRRDLDTGISDHLSLFQDRNMQISSFSGLSLDPVQPKKNTAVNSSQNVSRIGARKRFTTQQKLMSYSEPEKINEVGVEDQYNFRTENSTKRTVGSFADRWKFFEETSKCAQPKVPPKVVSSSQSEETSEIAINRDYAKSSEGQESKRALAVSGQNPADENGFPDKVTTERRQRLGTFAEYEASWKEQKTQLERKNSGRCHSADNILDADLEQNPKAQYIHERSKSSPTTDFYAQAAAVESKQQSESVRRDTENSNSTHCRSSTGDSPTRTVEAGDQCGATNEQEVLTCNTKWKPHDKSFPIPETSNESQQSRARSGTLPNDYRFAHENVNQGNRDISFSAVPISEACPDFSNADSDQLQDHPSVFKKRSAAPQRPPPPKLENKYWRQNGSSSSLATSSESLLTAQARRAQSYSPSSQDTFPPQSLQKQSPSTYPDKNPSIHIYDYQLSVPPENDRYHLEKKYFESELSSKSHLQIPGMEPSRSPSPQFAPQKLTDKPPLLVPEENLSRIERVIDNTTVKMVPIKIVHSETHAEKESRHNLLSAIEPTALPTGLAKDQLKTLSTSEQSYSRFCAYTRQESREEEESRVTDLYSCQRNAEDDNENDVSSLAPSNAKSKDAIYADLKSEELVREIVDKDKSLADILDPNAKMRTTMDLMEGIFPKDEHLLEEAQQRRKHLPKIPSPRSTDDKKDEQNVPSAVSLTTSSTYYSTSAAKAELLIKMKDMQEQQHIAENSEDELGQNLSEKKQELIDSISKKLQVLRDAKETLLEDVQCNNALGEEVEIIVKEVCKPNEFDKFRMFIGDLEKIVNLLLSLSGRLARVENALNNLDETVSPEERKTLLEKRKLLTRQHEDAKELKENLDRRERTVYEILANYLNEENLADYEHFVKMKSALILEQRELEDKIKLGEEQLKCLTDSLPLDRIK

Target ORF information:

RefSeq Version	XM_018091331.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091331.1

ORF Insert Sequence:

ATGGAGCCCA GAGCTGGGCA GGAGAGGTTT CTGCCTGAGG ATACAAGGGT TTATTATGGT 
TTGTTGGATC AAGGGCACAC AGTGGTGGAG AGGAAACTAA TGGAAGGCTA CAGGCTGGTG 
GATGTTATTC TTACTGGAGG GGCTCCTTGG GGATTTACTT TGAAAGGTGG CAAAGAACAT 
GGAGAGCCTC TTATCATTAC CAAGGTGGAA GATGGAAACA AAGCTGCTGA CATATTAATG 
GCTGGAGATG AAATTGTCAA CATAAATGAT CAGCAGCTTT TAGGTTATCG TCAGGAGGCA 
ATATGTCTGG TTAAAGGATC ACACAAGATT CTAAAAATGA TAGTAAAACG CTCTTCATCT 
CATGATCTCT CAAATCCTTG GGACCAAACA AGCTTGCAAA GAAATTCTGG ACATTTTAGC 
TCCATGGGGA GTATGGACAG TATTGACCAA ACATATCAGT TTGGAAGACT TTCATCTGCT 
AAATCAAATA ACAGTATAGA TTATGTAGGA AGCCAGAATA AGAGAGACTC AGCATATGGT 
TCTTTTTCTG CAAGTTTCAG CACTCCAGAT CATACCTTAT CTAAAACTGC TTCTGCATCA 
ACAGAAAATA TTCTATATAA GAACAATGAG TGGGACAACA CAAAGCTAGG TTATGGCAAG 
ACAAGTCCAT CTATGAATGA AGTCAGACGT TCTGCAGATA GACAGGTATT ACAGTCTACT 
TCTATTAATG AGACAAGCAA AATACAGAGG ACAGAAGATA ACACTGAACC TAGATATTCT 
GGGCGATCAA ACTTTGGTCC TGTATGGAAT ATTCCTGATA AAAAAAAGAC AGCCTCACCT 
CCCCCTCCAC CACCACCACA GCGCAGTGAT AGTTATGCAG TCACTAAAAT CCATGAGAAG 
CCTACCAACC TTATGCACTT GGATGCTTCA AGCACTCAGC ACTTTAATGT TGCAAATAGG 
TCTCAAGCAA AACCTGACTG GAGCTTAGAA ATATCTGAAC AACAAAGACC TATAAGAGCA 
CATGACAGGA CTGTAGCTGA TACAAGAAGA ACCAGCAATT CCTCTTACCA TGCTGGCTTA 
AATGCAGATC AAGGGTTGTC CCCCTATAAG GACAAATATC CAAGTAATTT GCCCAATGTC 
AGTAGAATAC AGGCTTCATT ATCTACCAAT GATGTCAGGT TTGCACAACC TGCTTACAAC 
TATCATCACC AGCGGCAATA CAGTGATGAA AGCACTCTTT TTCAGAGCAC AAGAACATCA 
GCACAGCATA AATCCCAACA ACAGCCAATG AAATATGAAT CTAGTGTAAA TCAGGTGCCA 
TCTGATTTGA CTTATGTTTA CCATCCTCAT CAATTTAGAG CACCTGCTAC TTCTGCAGGT 
TTTAGTAGTG GGAAACAAAA TGTAGAAAAC AATGGGCAGA ACCATTTTCA CGTTGTGAGT 
ATAAAACATC CTCAGGGAAA TACAACACAC CATCAGTTCA AGGAGGAGGA AAACTATGCA 
CCAGAGGTGA ACTCTGGAAG AAAATCTGTC CAACCTGAAA ATACTGTCAT TAGCATTGAA 
ATACCAGCTT ACAGTCTACC TCAGGAAAGT TTTGAACCTT CACAAATAAA CTGTGAAAAG 
AACTACCTAA AAATATCTGA GAAAAAGGAT AATTATTTAG GTAATAATGA ACAAATAATC 
AATAAAACCA CTGGATATTC AGAAGAAAAA TGTAACGACA GATTTAGCCA GCTGGACCAT 
TCTGAAAAAG GTTCATATAG ATCAAGTCAA GATTATTCTT GGAGAAAGGA AGAAAATAAG 
ATAACACCTC TAGTAACACC AATGTTGCAT TCTTTAGCTC AAGAAGGAAG AAATCGATCT 
GAAAGTTTCC CAGATACTGG AAATGAAAAA CTGTCTTTTC CAGATGCGGG CAAACAGTCT 
CGTAGAAGTG ATCGGTTTGC AACGACTCTG AGAAATGAAA TTCAGCAGAG GCGAGCTCGT 
CTGCAAAAGA GCAAAAGTAC AGCTGCCTTG ACCGAGTCAA ACGAAACAGA GACCTCTGAC 
AACTGGAAAC AAGACTCACT GGAAAGCATG AGCCCAACTT CTGAAGGTTC TTTTTCAAGC 
ACCTACAGAA GTCATCTGCA AGAAGCCCAG GCCCGTGTTT TAAGAGCTAC TTCCTTTAAA 
AGAAGAGACC TTGATACAGG TATTTCTGAT CATCTTTCAC TGTTTCAAGA TCGAAATATG 
CAGATCTCAA GCTTTTCAGG CCTATCTTTA GATCCTGTTC AACCTAAAAA AAACACAGCT 
GTTAACAGCT CACAAAATGT ATCTCGTATT GGAGCAAGAA AGAGGTTCAC AACTCAGCAG 
AAGCTGATGT CCTACTCAGA GCCTGAGAAA ATTAATGAAG TTGGTGTTGA AGACCAATAC 
AATTTCCGTA CTGAAAATTC TACTAAAAGA ACTGTGGGAT CTTTTGCAGA CAGATGGAAA 
TTCTTTGAAG AGACCAGCAA ATGTGCACAA CCAAAGGTTC CACCAAAAGT TGTTTCTTCT 
AGCCAGTCAG AAGAAACTTC TGAAATAGCC ATTAACAGGG ATTATGCCAA GAGTTCTGAG 
GGGCAAGAGA GCAAAAGGGC TTTGGCAGTG TCTGGACAAA ATCCTGCTGA TGAAAACGGC 
TTCCCTGATA AAGTTACAAC AGAACGTCGT CAAAGATTAG GGACTTTTGC AGAATATGAG 
GCATCTTGGA AAGAACAGAA AACACAGCTG GAGAGAAAAA ATTCTGGCAG ATGTCATTCG 
GCTGACAACA TCCTTGATGC TGATCTTGAG CAGAATCCTA AAGCTCAGTA TATTCATGAG 
CGGTCCAAGT CTTCTCCTAC CACAGATTTT TATGCACAGG CAGCAGCCGT TGAGTCAAAG 
CAGCAGTCAG AGAGCGTGAG GAGAGACACC GAGAATTCTA ACTCGACACA CTGCCGCAGT 
AGCACAGGGG ATTCTCCCAC AAGAACTGTT GAAGCTGGCG ATCAGTGTGG TGCTACCAAT 
GAACAAGAGG TTTTAACCTG CAATACTAAA TGGAAGCCAC ATGATAAATC GTTTCCGATC 
CCAGAAACAT CCAATGAATC CCAGCAGAGC AGAGCAAGAT CAGGGACGCT ACCAAATGAT 
TATAGATTTG CTCATGAAAA TGTGAATCAA GGAAATCGGG ATATCAGCTT TTCTGCTGTT 
CCTATATCTG AAGCCTGCCC TGATTTCTCC AATGCAGACA GCGATCAGTT GCAAGATCAC 
CCATCTGTGT TTAAGAAGCG CAGTGCTGCC CCACAAAGGC CTCCTCCGCC TAAACTTGAA 
AACAAATATT GGCGGCAGAA TGGCTCCTCA TCTTCCCTTG CCACATCCTC AGAGTCATTG 
CTGACAGCCC AAGCCAGGAG GGCACAATCC TATTCCCCTA GCAGCCAAGA CACATTTCCT 
CCTCAGTCTC TTCAGAAACA AAGCCCAAGT ACATATCCTG ATAAGAATCC TAGCATTCAT 
ATATATGATT ATCAATTATC TGTGCCACCA GAGAATGACA GGTATCATCT AGAAAAGAAA 
TACTTTGAAT CTGAACTGTC CTCAAAGTCA CACTTGCAGA TTCCTGGAAT GGAACCGTCT 
CGATCACCCT CTCCTCAGTT TGCTCCACAG AAGCTTACAG ACAAGCCTCC CCTTCTGGTT 
CCAGAAGAAA ACTTGTCAAG GATTGAAAGG GTGATCGATA ACACTACAGT GAAAATGGTC 
CCCATAAAGA TTGTTCACTC TGAAACTCAT GCTGAGAAGG AAAGCAGACA CAATTTGCTG 
AGTGCTATTG AGCCCACTGC CTTACCAACT GGTCTTGCCA AGGACCAACT AAAGACTTTG 
AGCACATCAG AACAATCCTA CTCCCGATTT TGTGCTTACA CCAGGCAGGA ATCTAGAGAG 
GAAGAGGAAT CCAGAGTGAC TGACCTTTAC TCATGCCAGA GAAATGCAGA AGATGATAAT 
GAAAATGATG TATCTTCTCT TGCACCCTCA AATGCTAAAA GCAAAGATGC AATCTACGCG 
GATTTAAAGT CAGAGGAGCT GGTGAGGGAA ATTGTAGATA AAGATAAATC TCTTGCGGAC 
ATTTTAGATC CAAATGCCAA GATGAGAACT ACCATGGACT TAATGGAGGG AATCTTTCCA 
AAAGATGAAC ATCTTCTAGA AGAAGCTCAG CAGCGTAGAA AACATCTCCC AAAAATACCA 
TCCCCAAGAT CAACAGATGA CAAAAAAGAT GAGCAAAATG TCCCATCAGC TGTTTCTCTG 
ACAACCAGTT CCACGTATTA CAGCACTTCA GCAGCCAAAG CCGAGCTCCT TATTAAAATG 
AAAGACATGC AGGAGCAGCA GCACATTGCG GAGAATTCAG AGGATGAGCT GGGTCAGAAC 
CTATCCGAGA AAAAGCAAGA ACTGATTGAC AGCATTAGCA AGAAGCTGCA GGTCCTGCGA 
GATGCAAAGG AAACCCTGTT AGAAGATGTC CAGTGTAACA ATGCTCTGGG GGAAGAGGTA 
GAAATCATTG TGAAAGAAGT CTGCAAACCA AATGAATTTG ACAAATTCAG GATGTTCATT 
GGGGATCTGG AGAAAATCGT CAATCTTCTG CTGTCACTTT CTGGGCGACT TGCTCGAGTG 
GAAAATGCTC TTAATAACTT GGATGAAACC GTGTCTCCTG AAGAGCGGAA AACACTGTTG 
GAAAAAAGGA AATTACTTAC GAGACAGCAT GAAGACGCAA AGGAACTGAA GGAGAACCTA 
GACCGGCGTG AGCGCACCGT TTATGAGATT TTGGCTAATT ACCTTAATGA GGAAAACCTT 
GCAGATTATG AGCACTTTGT GAAGATGAAG TCGGCACTCA TCCTTGAGCA GCGAGAGCTG 
GAAGACAAAA TCAAGCTTGG AGAGGAGCAG CTCAAATGCC TGACCGATAG TCTTCCATTG 
GATCGTATCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2 ( XM_018091332.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946821.1 Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X3, mRNA.

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CloneID	OXa42598
Clone ID Related Accession (Same CDS sequence)	XM_018091332.1
Accession Version	XM_018091332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACAG TAACTCCGCA GATGTCAAAT GCCAGCCCAT TTTGGAATGC CCGATATCGC 
TCCAGCTCTT CATCTCATGA TCTCTCAAAT CCTTGGGACC AAACAAGCTT GCAAAGAAAT 
TCTGGACATT TTAGCTCCAT GGGGAGTATG GACAGTATTG ACCAAACATA TCAGTTTGGA 
AGACTTTCAT CTGCTAAATC AAATAACAGT ATAGATTATG TAGGAAGCCA GAATAAGAGA 
GACTCAGCAT ATGGTTCTTT TTCTGCAAGT TTCAGCACTC CAGATCATAC CTTATCTAAA 
ACTGCTTCTG CATCAACAGA AAATATTCTA TATAAGAACA ATGAGTGGGA CAACACAAAG 
CTAGGTTATG GCAAGACAAG TCCATCTATG AATGAAGTCA GACGTTCTGC AGATAGACAG 
GTATTACAGT CTACTTCTAT TAATGAGACA AGCAAAATAC AGAGGACAGA AGATAACACT 
GAACCTAGAT ATTCTGGGCG ATCAAACTTT GGTCCTGTAT GGAATATTCC TGATAAAAAA 
AAGACAGCCT CACCTCCCCC TCCACCACCA CCACAGCGCA GTGATAGTTA TGCAGTCACT 
AAAATCCATG AGAAGCCTAC CAACCTTATG CACTTGGATG CTTCAAGCAC TCAGCACTTT 
AATGTTGCAA ATAGGTCTCA AGCAAAACCT GACTGGAGCT TAGAAATATC TGAACAACAA 
AGACCTATAA GAGCACATGA CAGGACTGTA GCTGATACAA GAAGAACCAG CAATTCCTCT 
TACCATGCTG GCTTAAATGC AGATCAAGGG TTGTCCCCCT ATAAGGACAA ATATCCAAGT 
AATTTGCCCA ATGTCAGTAG AATACAGGCT TCATTATCTA CCAATGATGT CAGGTTTGCA 
CAACCTGCTT ACAACTATCA TCACCAGCGG CAATACAGTG ATGAAAGCAC TCTTTTTCAG 
AGCACAAGAA CATCAGCACA GCATAAATCC CAACAACAGC CAATGAAATA TGAATCTAGT 
GTAAATCAGG TGCCATCTGA TTTGACTTAT GTTTACCATC CTCATCAATT TAGAGCACCT 
GCTACTTCTG CAGGTTTTAG TAGTGGGAAA CAAAATGTAG AAAACAATGG GCAGAACCAT 
TTTCACGTTG TGAGTATAAA ACATCCTCAG GGAAATACAA CACACCATCA GTTCAAGGAG 
GAGGAAAACT ATGCACCAGA GGTGAACTCT GGAAGAAAAT CTGTCCAACC TGAAAATACT 
GTCATTAGCA TTGAAATACC AGCTTACAGT CTACCTCAGG AAAGTTTTGA ACCTTCACAA 
ATAAACTGTG AAAAGAACTA CCTAAAAATA TCTGAGAAAA AGGATAATTA TTTAGGTAAT 
AATGAACAAA TAATCAATAA AACCACTGGA TATTCAGAAG AAAAATGTAA CGACAGATTT 
AGCCAGCTGG ACCATTCTGA AAAAGGTTCA TATAGATCAA GTCAAGATTA TTCTTGGAGA 
AAGGAAGAAA ATAAGATAAC ACCTCTAGTA ACACCAATGT TGCATTCTTT AGCTCAAGAA 
GGAAGAAATC GATCTGAAAG TTTCCCAGAT ACTGGAAATG AAAAACTGTC TTTTCCAGAT 
GCGGGCAAAC AGTCTCGTAG AAGTGATCGG TTTGCAACGA CTCTGAGAAA TGAAATTCAG 
CAGAGGCGAG CTCGTCTGCA AAAGAGCAAA AGTACAGCTG CCTTGACCGA GTCAAACGAA 
ACAGAGACCT CTGACAACTG GAAACAAGAC TCACTGGAAA GCATGAGCCC AACTTCTGAA 
GGTTCTTTTT CAAGCACCTA CAGAAGTCAT CTGCAAGAAG CCCAGGCCCG TGTTTTAAGA 
GCTACTTCCT TTAAAAGAAG AGACCTTGAT ACAGGTATTT CTGATCATCT TTCACTGTTT 
CAAGATCGAA ATATGCAGAT CTCAAGCTTT TCAGGCCTAT CTTTAGATCC TGTTCAACCT 
AAAAAAAACA CAGCTGTTAA CAGCTCACAA AATGTATCTC GTATTGGAGC AAGAAAGAGG 
TTCACAACTC AGCAGAAGCT GATGTCCTAC TCAGAGCCTG AGAAAATTAA TGAAGTTGGT 
GTTGAAGACC AATACAATTT CCGTACTGAA AATTCTACTA AAAGAACTGT GGGATCTTTT 
GCAGACAGAT GGAAATTCTT TGAAGAGACC AGCAAATGTG CACAACCAAA GGTTCCACCA 
AAAGTTGTTT CTTCTAGCCA GTCAGAAGAA ACTTCTGAAA TAGCCATTAA CAGGGATTAT 
GCCAAGAGTT CTGAGGGGCA AGAGAGCAAA AGGGCTTTGG CAGTGTCTGG ACAAAATCCT 
GCTGATGAAA ACGGCTTCCC TGATAAAGTT ACAACAGAAC GTCGTCAAAG ATTAGGGACT 
TTTGCAGAAT ATGAGGCATC TTGGAAAGAA CAGAAAACAC AGCTGGAGAG AAAAAATTCT 
GGCAGATGTC ATTCGGCTGA CAACATCCTT GATGCTGATC TTGAGCAGAA TCCTAAAGCT 
CAGTATATTC ATGAGCGGTC CAAGTCTTCT CCTACCACAG ATTTTTATGC ACAGGCAGCA 
GCCGTTGAGT CAAAGCAGCA GTCAGAGAGC GTGAGGAGAG ACACCGAGAA TTCTAACTCG 
ACACACTGCC GCAGTAGCAC AGGGGATTCT CCCACAAGAA CTGTTGAAGC TGGCGATCAG 
TGTGGTGCTA CCAATGAACA AGAGGTTTTA ACCTGCAATA CTAAATGGAA GCCACATGAT 
AAATCGTTTC CGATCCCAGA AACATCCAAT GAATCCCAGC AGAGCAGAGC AAGATCAGGG 
ACGCTACCAA ATGATTATAG ATTTGCTCAT GAAAATGTGA ATCAAGGAAA TCGGGATATC 
AGCTTTTCTG CTGTTCCTAT ATCTGAAGCC TGCCCTGATT TCTCCAATGC AGACAGCGAT 
CAGTTGCAAG ATCACCCATC TGTGTTTAAG AAGCGCAGTG CTGCCCCACA AAGGCCTCCT 
CCGCCTAAAC TTGAAAACAA ATATTGGCGG CAGAATGGCT CCTCATCTTC CCTTGCCACA 
TCCTCAGAGT CATTGCTGAC AGCCCAAGCC AGGAGGGCAC AATCCTATTC CCCTAGCAGC 
CAAGACACAT TTCCTCCTCA GTCTCTTCAG AAACAAAGCC CAAGTACATA TCCTGATAAG 
AATCCTAGCA TTCATATATA TGATTATCAA TTATCTGTGC CACCAGAGAA TGACAGGTAT 
CATCTAGAAA AGAAATACTT TGAATCTGAA CTGTCCTCAA AGTCACACTT GCAGATTCCT 
GGAATGGAAC CGTCTCGATC ACCCTCTCCT CAGTTTGCTC CACAGAAGCT TACAGACAAG 
CCTCCCCTTC TGGTTCCAGA AGAAAACTTG TCAAGGATTG AAAGGGTGAT CGATAACACT 
ACAGTGAAAA TGGTCCCCAT AAAGATTGTT CACTCTGAAA CTCATGCTGA GAAGGAAAGC 
AGACACAATT TGCTGAGTGC TATTGAGCCC ACTGCCTTAC CAACTGGTCT TGCCAAGGAC 
CAACTAAAGA CTTTGAGCAC ATCAGAACAA TCCTACTCCC GATTTTGTGC TTACACCAGG 
CAGGAATCTA GAGAGGAAGA GGAATCCAGA GTGACTGACC TTTACTCATG CCAGAGAAAT 
GCAGAAGATG ATAATGAAAA TGATGTATCT TCTCTTGCAC CCTCAAATGC TAAAAGCAAA 
GATGCAATCT ACGCGGATTT AAAGTCAGAG GAGCTGGTGA GGGAAATTGT AGATAAAGAT 
AAATCTCTTG CGGACATTTT AGATCCAAAT GCCAAGATGA GAACTACCAT GGACTTAATG 
GAGGGAATCT TTCCAAAAGA TGAACATCTT CTAGAAGAAG CTCAGCAGCG TAGAAAACAT 
CTCCCAAAAA TACCATCCCC AAGATCAACA GATGACAAAA AAGATGAGCA AAATGTCCCA 
TCAGCTGTTT CTCTGACAAC CAGTTCCACG TATTACAGCA CTTCAGCAGC CAAAGCCGAG 
CTCCTTATTA AAATGAAAGA CATGCAGGAG CAGCAGCACA TTGCGGAGAA TTCAGAGGAT 
GAGCTGGGTC AGAACCTATC CGAGAAAAAG CAAGAACTGA TTGACAGCAT TAGCAAGAAG 
CTGCAGGTCC TGCGAGATGC AAAGGAAACC CTGTTAGAAG ATGTCCAGTG TAACAATGCT 
CTGGGGGAAG AGGTAGAAAT CATTGTGAAA GAAGTCTGCA AACCAAATGA ATTTGACAAA 
TTCAGGATGT TCATTGGGGA TCTGGAGAAA ATCGTCAATC TTCTGCTGTC ACTTTCTGGG 
CGACTTGCTC GAGTGGAAAA TGCTCTTAAT AACTTGGATG AAACCGTGTC TCCTGAAGAG 
CGGAAAACAC TGTTGGAAAA AAGGAAATTA CTTACGAGAC AGCATGAAGA CGCAAAGGAA 
CTGAAGGAGA ACCTAGACCG GCGTGAGCGC ACCGTTTATG AGATTTTGGC TAATTACCTT 
AATGAGGAAA ACCTTGCAGA TTATGAGCAC TTTGTGAAGA TGAAGTCGGC ACTCATCCTT 
GAGCAGCGAG AGCTGGAAGA CAAAATCAAG CTTGGAGAGG AGCAGCTCAA ATGCCTGACC 
GATAGTCTTC CATTGGATCG TATCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946821.1
CDS	203..4852
Translation	MDTVTPQMSNASPFWNARYRSSSSSHDLSNPWDQTSLQRNSGHFSSMGSMDSIDQTYQFGRLSSAKSNNSIDYVGSQNKRDSAYGSFSASFSTPDHTLSKTASASTENILYKNNEWDNTKLGYGKTSPSMNEVRRSADRQVLQSTSINETSKIQRTEDNTEPRYSGRSNFGPVWNIPDKKKTASPPPPPPPQRSDSYAVTKIHEKPTNLMHLDASSTQHFNVANRSQAKPDWSLEISEQQRPIRAHDRTVADTRRTSNSSYHAGLNADQGLSPYKDKYPSNLPNVSRIQASLSTNDVRFAQPAYNYHHQRQYSDESTLFQSTRTSAQHKSQQQPMKYESSVNQVPSDLTYVYHPHQFRAPATSAGFSSGKQNVENNGQNHFHVVSIKHPQGNTTHHQFKEEENYAPEVNSGRKSVQPENTVISIEIPAYSLPQESFEPSQINCEKNYLKISEKKDNYLGNNEQIINKTTGYSEEKCNDRFSQLDHSEKGSYRSSQDYSWRKEENKITPLVTPMLHSLAQEGRNRSESFPDTGNEKLSFPDAGKQSRRSDRFATTLRNEIQQRRARLQKSKSTAALTESNETETSDNWKQDSLESMSPTSEGSFSSTYRSHLQEAQARVLRATSFKRRDLDTGISDHLSLFQDRNMQISSFSGLSLDPVQPKKNTAVNSSQNVSRIGARKRFTTQQKLMSYSEPEKINEVGVEDQYNFRTENSTKRTVGSFADRWKFFEETSKCAQPKVPPKVVSSSQSEETSEIAINRDYAKSSEGQESKRALAVSGQNPADENGFPDKVTTERRQRLGTFAEYEASWKEQKTQLERKNSGRCHSADNILDADLEQNPKAQYIHERSKSSPTTDFYAQAAAVESKQQSESVRRDTENSNSTHCRSSTGDSPTRTVEAGDQCGATNEQEVLTCNTKWKPHDKSFPIPETSNESQQSRARSGTLPNDYRFAHENVNQGNRDISFSAVPISEACPDFSNADSDQLQDHPSVFKKRSAAPQRPPPPKLENKYWRQNGSSSSLATSSESLLTAQARRAQSYSPSSQDTFPPQSLQKQSPSTYPDKNPSIHIYDYQLSVPPENDRYHLEKKYFESELSSKSHLQIPGMEPSRSPSPQFAPQKLTDKPPLLVPEENLSRIERVIDNTTVKMVPIKIVHSETHAEKESRHNLLSAIEPTALPTGLAKDQLKTLSTSEQSYSRFCAYTRQESREEEESRVTDLYSCQRNAEDDNENDVSSLAPSNAKSKDAIYADLKSEELVREIVDKDKSLADILDPNAKMRTTMDLMEGIFPKDEHLLEEAQQRRKHLPKIPSPRSTDDKKDEQNVPSAVSLTTSSTYYSTSAAKAELLIKMKDMQEQQHIAENSEDELGQNLSEKKQELIDSISKKLQVLRDAKETLLEDVQCNNALGEEVEIIVKEVCKPNEFDKFRMFIGDLEKIVNLLLSLSGRLARVENALNNLDETVSPEERKTLLEKRKLLTRQHEDAKELKENLDRRERTVYEILANYLNEENLADYEHFVKMKSALILEQRELEDKIKLGEEQLKCLTDSLPLDRIK

Target ORF information:

RefSeq Version	XM_018091332.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091332.1

ORF Insert Sequence:

ATGGATACAG TAACTCCGCA GATGTCAAAT GCCAGCCCAT TTTGGAATGC CCGATATCGC 
TCCAGCTCTT CATCTCATGA TCTCTCAAAT CCTTGGGACC AAACAAGCTT GCAAAGAAAT 
TCTGGACATT TTAGCTCCAT GGGGAGTATG GACAGTATTG ACCAAACATA TCAGTTTGGA 
AGACTTTCAT CTGCTAAATC AAATAACAGT ATAGATTATG TAGGAAGCCA GAATAAGAGA 
GACTCAGCAT ATGGTTCTTT TTCTGCAAGT TTCAGCACTC CAGATCATAC CTTATCTAAA 
ACTGCTTCTG CATCAACAGA AAATATTCTA TATAAGAACA ATGAGTGGGA CAACACAAAG 
CTAGGTTATG GCAAGACAAG TCCATCTATG AATGAAGTCA GACGTTCTGC AGATAGACAG 
GTATTACAGT CTACTTCTAT TAATGAGACA AGCAAAATAC AGAGGACAGA AGATAACACT 
GAACCTAGAT ATTCTGGGCG ATCAAACTTT GGTCCTGTAT GGAATATTCC TGATAAAAAA 
AAGACAGCCT CACCTCCCCC TCCACCACCA CCACAGCGCA GTGATAGTTA TGCAGTCACT 
AAAATCCATG AGAAGCCTAC CAACCTTATG CACTTGGATG CTTCAAGCAC TCAGCACTTT 
AATGTTGCAA ATAGGTCTCA AGCAAAACCT GACTGGAGCT TAGAAATATC TGAACAACAA 
AGACCTATAA GAGCACATGA CAGGACTGTA GCTGATACAA GAAGAACCAG CAATTCCTCT 
TACCATGCTG GCTTAAATGC AGATCAAGGG TTGTCCCCCT ATAAGGACAA ATATCCAAGT 
AATTTGCCCA ATGTCAGTAG AATACAGGCT TCATTATCTA CCAATGATGT CAGGTTTGCA 
CAACCTGCTT ACAACTATCA TCACCAGCGG CAATACAGTG ATGAAAGCAC TCTTTTTCAG 
AGCACAAGAA CATCAGCACA GCATAAATCC CAACAACAGC CAATGAAATA TGAATCTAGT 
GTAAATCAGG TGCCATCTGA TTTGACTTAT GTTTACCATC CTCATCAATT TAGAGCACCT 
GCTACTTCTG CAGGTTTTAG TAGTGGGAAA CAAAATGTAG AAAACAATGG GCAGAACCAT 
TTTCACGTTG TGAGTATAAA ACATCCTCAG GGAAATACAA CACACCATCA GTTCAAGGAG 
GAGGAAAACT ATGCACCAGA GGTGAACTCT GGAAGAAAAT CTGTCCAACC TGAAAATACT 
GTCATTAGCA TTGAAATACC AGCTTACAGT CTACCTCAGG AAAGTTTTGA ACCTTCACAA 
ATAAACTGTG AAAAGAACTA CCTAAAAATA TCTGAGAAAA AGGATAATTA TTTAGGTAAT 
AATGAACAAA TAATCAATAA AACCACTGGA TATTCAGAAG AAAAATGTAA CGACAGATTT 
AGCCAGCTGG ACCATTCTGA AAAAGGTTCA TATAGATCAA GTCAAGATTA TTCTTGGAGA 
AAGGAAGAAA ATAAGATAAC ACCTCTAGTA ACACCAATGT TGCATTCTTT AGCTCAAGAA 
GGAAGAAATC GATCTGAAAG TTTCCCAGAT ACTGGAAATG AAAAACTGTC TTTTCCAGAT 
GCGGGCAAAC AGTCTCGTAG AAGTGATCGG TTTGCAACGA CTCTGAGAAA TGAAATTCAG 
CAGAGGCGAG CTCGTCTGCA AAAGAGCAAA AGTACAGCTG CCTTGACCGA GTCAAACGAA 
ACAGAGACCT CTGACAACTG GAAACAAGAC TCACTGGAAA GCATGAGCCC AACTTCTGAA 
GGTTCTTTTT CAAGCACCTA CAGAAGTCAT CTGCAAGAAG CCCAGGCCCG TGTTTTAAGA 
GCTACTTCCT TTAAAAGAAG AGACCTTGAT ACAGGTATTT CTGATCATCT TTCACTGTTT 
CAAGATCGAA ATATGCAGAT CTCAAGCTTT TCAGGCCTAT CTTTAGATCC TGTTCAACCT 
AAAAAAAACA CAGCTGTTAA CAGCTCACAA AATGTATCTC GTATTGGAGC AAGAAAGAGG 
TTCACAACTC AGCAGAAGCT GATGTCCTAC TCAGAGCCTG AGAAAATTAA TGAAGTTGGT 
GTTGAAGACC AATACAATTT CCGTACTGAA AATTCTACTA AAAGAACTGT GGGATCTTTT 
GCAGACAGAT GGAAATTCTT TGAAGAGACC AGCAAATGTG CACAACCAAA GGTTCCACCA 
AAAGTTGTTT CTTCTAGCCA GTCAGAAGAA ACTTCTGAAA TAGCCATTAA CAGGGATTAT 
GCCAAGAGTT CTGAGGGGCA AGAGAGCAAA AGGGCTTTGG CAGTGTCTGG ACAAAATCCT 
GCTGATGAAA ACGGCTTCCC TGATAAAGTT ACAACAGAAC GTCGTCAAAG ATTAGGGACT 
TTTGCAGAAT ATGAGGCATC TTGGAAAGAA CAGAAAACAC AGCTGGAGAG AAAAAATTCT 
GGCAGATGTC ATTCGGCTGA CAACATCCTT GATGCTGATC TTGAGCAGAA TCCTAAAGCT 
CAGTATATTC ATGAGCGGTC CAAGTCTTCT CCTACCACAG ATTTTTATGC ACAGGCAGCA 
GCCGTTGAGT CAAAGCAGCA GTCAGAGAGC GTGAGGAGAG ACACCGAGAA TTCTAACTCG 
ACACACTGCC GCAGTAGCAC AGGGGATTCT CCCACAAGAA CTGTTGAAGC TGGCGATCAG 
TGTGGTGCTA CCAATGAACA AGAGGTTTTA ACCTGCAATA CTAAATGGAA GCCACATGAT 
AAATCGTTTC CGATCCCAGA AACATCCAAT GAATCCCAGC AGAGCAGAGC AAGATCAGGG 
ACGCTACCAA ATGATTATAG ATTTGCTCAT GAAAATGTGA ATCAAGGAAA TCGGGATATC 
AGCTTTTCTG CTGTTCCTAT ATCTGAAGCC TGCCCTGATT TCTCCAATGC AGACAGCGAT 
CAGTTGCAAG ATCACCCATC TGTGTTTAAG AAGCGCAGTG CTGCCCCACA AAGGCCTCCT 
CCGCCTAAAC TTGAAAACAA ATATTGGCGG CAGAATGGCT CCTCATCTTC CCTTGCCACA 
TCCTCAGAGT CATTGCTGAC AGCCCAAGCC AGGAGGGCAC AATCCTATTC CCCTAGCAGC 
CAAGACACAT TTCCTCCTCA GTCTCTTCAG AAACAAAGCC CAAGTACATA TCCTGATAAG 
AATCCTAGCA TTCATATATA TGATTATCAA TTATCTGTGC CACCAGAGAA TGACAGGTAT 
CATCTAGAAA AGAAATACTT TGAATCTGAA CTGTCCTCAA AGTCACACTT GCAGATTCCT 
GGAATGGAAC CGTCTCGATC ACCCTCTCCT CAGTTTGCTC CACAGAAGCT TACAGACAAG 
CCTCCCCTTC TGGTTCCAGA AGAAAACTTG TCAAGGATTG AAAGGGTGAT CGATAACACT 
ACAGTGAAAA TGGTCCCCAT AAAGATTGTT CACTCTGAAA CTCATGCTGA GAAGGAAAGC 
AGACACAATT TGCTGAGTGC TATTGAGCCC ACTGCCTTAC CAACTGGTCT TGCCAAGGAC 
CAACTAAAGA CTTTGAGCAC ATCAGAACAA TCCTACTCCC GATTTTGTGC TTACACCAGG 
CAGGAATCTA GAGAGGAAGA GGAATCCAGA GTGACTGACC TTTACTCATG CCAGAGAAAT 
GCAGAAGATG ATAATGAAAA TGATGTATCT TCTCTTGCAC CCTCAAATGC TAAAAGCAAA 
GATGCAATCT ACGCGGATTT AAAGTCAGAG GAGCTGGTGA GGGAAATTGT AGATAAAGAT 
AAATCTCTTG CGGACATTTT AGATCCAAAT GCCAAGATGA GAACTACCAT GGACTTAATG 
GAGGGAATCT TTCCAAAAGA TGAACATCTT CTAGAAGAAG CTCAGCAGCG TAGAAAACAT 
CTCCCAAAAA TACCATCCCC AAGATCAACA GATGACAAAA AAGATGAGCA AAATGTCCCA 
TCAGCTGTTT CTCTGACAAC CAGTTCCACG TATTACAGCA CTTCAGCAGC CAAAGCCGAG 
CTCCTTATTA AAATGAAAGA CATGCAGGAG CAGCAGCACA TTGCGGAGAA TTCAGAGGAT 
GAGCTGGGTC AGAACCTATC CGAGAAAAAG CAAGAACTGA TTGACAGCAT TAGCAAGAAG 
CTGCAGGTCC TGCGAGATGC AAAGGAAACC CTGTTAGAAG ATGTCCAGTG TAACAATGCT 
CTGGGGGAAG AGGTAGAAAT CATTGTGAAA GAAGTCTGCA AACCAAATGA ATTTGACAAA 
TTCAGGATGT TCATTGGGGA TCTGGAGAAA ATCGTCAATC TTCTGCTGTC ACTTTCTGGG 
CGACTTGCTC GAGTGGAAAA TGCTCTTAAT AACTTGGATG AAACCGTGTC TCCTGAAGAG 
CGGAAAACAC TGTTGGAAAA AAGGAAATTA CTTACGAGAC AGCATGAAGA CGCAAAGGAA 
CTGAAGGAGA ACCTAGACCG GCGTGAGCGC ACCGTTTATG AGATTTTGGC TAATTACCTT 
AATGAGGAAA ACCTTGCAGA TTATGAGCAC TTTGTGAAGA TGAAGTCGGC ACTCATCCTT 
GAGCAGCGAG AGCTGGAAGA CAAAATCAAG CTTGGAGAGG AGCAGCTCAA ATGCCTGACC 
GATAGTCTTC CATTGGATCG TATCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2 ( NM_001078710.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072178.1 Xenopus tropicalis shroom family member 2 (shroom2), mRNA.

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CloneID	OXa01957
Clone ID Related Accession (Same CDS sequence)	NM_001078710.1
Accession Version	NM_001078710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ886532.1. ##Evidence-Data-START## Transcript exon combination :: DQ886532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGTTGGTGT TAGGCTGCCT GCAATGTTTT ACCTCTGCCT GGGGATTTGC TTCTAGGAGG 
ATCTGTCATT CATCGTCTGC TACTCCTGGA CAGCACAGTG TTGGCTTGGG GCACGGCTAC 
CAGCATGGAG CCCAGAGCTG GGCAGAGAGG TTTCTGCTGA GATACAAGGG TTTATTATGG 
TTTGTTGGAT CAAGGGCACA CAGTGGTGGA GAGGAAACTA ATGGAAAGCT ACAGGCTGGT 
GGATGTTATT CTTACTGGAG GGGCTCCTTG GGGATTTACT TTGAAAGGGG CAAAGAACAT 
GGAGAGCCTC TTATCATTAC CAAGGTGGAA GATGGAAACA AAGCTGCTGA CATATTAATG 
GCTGGAGATG AAATTGTCAA CATAAATGAT CAGCAGCTTT TAGGTTATCG TCAGGAGGCA 
ATATGTCTGG TTAAAGGATC ACACAAGATT CTAAAAATGA TAGTAAAACG GAGAAATGAC 
ATCTCATACA GGCCTCATTC CTGGCACTCA AATAAGTTGA TTGAAAGTGC TATGGATACA 
GTAACTCCGC AGATGTCAAA TGCCAGCCCA TTTTGGAATG CCCGATATCG CTCCAGCTCT 
TCATCTCATG ATCTCTCAAA TCCTTGGGAC CAAACAAGCT TGCAAAGAAA TTCTGGACAT 
TTTAGCTCCA TGGGGAGTAT GGACAGTATT GACCAAACAT ATCAGTTTGG AAGACTTTCA 
TCTGCTAAAT CAAATAACAG TATAGATTAT GTAGGAAGCC AGAATAAGAG AGACTCAGCA 
TATGGTTCTT TTTCTGCAAG TTTCAGCACT CCAGATCATA CCTTATCTAA AACTGCTTCT 
GCATCAACAG AAAATATTCT ATATAAGAAC AATGAGTGGG ACAACACAAA GCTAGGTTAT 
GGCAAGACAA GTCCATCTAT GAATGAAGTC AGACGTTCTG CAGATAGACA GGTATTACAG 
TCTACTTCTA TTAATGAGAC AAGCAAAATA CAGAGGACAG AAGATAACAC TGAACCTAGA 
TATTCTGGGC GATCAAACTT TGGTCCTGTA TGGAATATTC CTGATAAAAA AAAGACAGCC 
TCACCTCCCC CTCCACCACC ACCACAGCGC AGTGATAGTT ATGCAGTCAC TAAAATCCAT 
GAGAAGCCTA CCAACCTTAT GCACTTGGAT GCTTCAAGCA CTCAGCACTT TAATGTTGCA 
AATAGGTCTC AAGCAAAACC TGACTGGAGC TTAGAAATAT CTGAACAACA AAGACCTATA 
AGAGCACATG ACAGGACTGT AGCTGATACA AGAAGAACCA GCAATTCCTC TTACCATGCT 
GGCTTAAATG CAGATCAAGG GTTGTCCCCC TATAAGGACA AATATCCAAG TAATTTGCCC 
AATGTCAGTA GAATACAGGC TTCATTATCT ACCAATGATG TCAGGTTTGC ACAACCTGCT 
TACAACTATC ATCACCAGCG GCAATACAGT GATGAAAGCA CTCTTTTTCA GAGCACAAGA 
ACATCAGCAC AGCATAAATC CCAACAACAG CCAATGAAAT ATGAATCTAG TGTAAATCAG 
GTGCCATCTG ATTTGACTTA TGTTTACCAT CCTCATCAAT TTAGAGCACC TGCTACTTCT 
GCAGGTTTTA GTAGTGGGAA ACAAAATGTA GAAAACAATG GGCAGAACCA TTTTCACGTT 
GTGAGTATAA AACATCCTCA GGGAAATACA ACACACCATC AGTTCAAGGA GGAGGAAAAC 
TATGCACCAG AGGTGAACTC TGGAAGAAAA TCTGTCCAAC CTGAAAATAC TGTCATTAGC 
ATTGAAATAC CAGCTTACAG TCTACCTCAG GAAAGTTTTG AACCTTCACA AATAAACTGT 
GAAAAGAACT ACCTAAAAAT ATCTGAGAAA AAGGATAATT ATTTAGGTAA TAATGAACAA 
ATAATCAATA AAACCACTGG ATATTCAGAA GAAAAATGTA ACGACAGATT TAGCCAGCTG 
GACCATTCTG AAAAAGGTTC ATATAGATCA AGTCAAGATT ATTCTTGGAG AAAGGAAGAA 
AATAAGATAA CACCTCTAGT AACACCAATG TTGCATTCTT TAGCTCAAGA AGGAAGAAAT 
CGATCTGAAA GTTTCCCAGA TACTGGAAAT GAAAAACTGT CTTTTCCAGA TGCGGGCAAA 
CAGTCTCGTA GAAGTGATCG GTTTGCAACG ACTCTGAGAA ATGAAATTCA GCAGAGGCGA 
GCTCGTCTGC AAAAGAGCAA AAGTACAGCT GCCTTGACCG AGTCAAACGA AACAGAGACC 
TCTGACAACT GGAAACAAGA CTCACTGGAA AGCATGAGCC CAACTTCTGA AGGTTCTTTT 
TCAAGCACCT ACAGAAGTCA TCTGCAAGAA GCCCAGGCCC GTGTTTTAAG AGCTACTTCC 
TTTAAAAGAA GAGACCTTGA TACAGGTATT TCTGATCATC TTTCACTGTT TCAAGATCGA 
AATATGCAGA TCTCAAGCTT TTCAGGCCTA TCTTTAGATC CTGTTCAACC TAAAAAAAAC 
ACAGCTGTTA ACAGCTCACA AAATGTATCT CGTATTGGAG CAAGAAAGAG GTTCACAACT 
CAGCAGAAGC TGATGTCCTA CTCAGAGCCT GAGAAAATTA ATGAAGTTGG TGTTGAAGAC 
CAATACAATT TCCGTACTGA AAATTCTACT AAAAGAACTG TGGGATCTTT TGCAGACAGA 
TGGAAATTCT TTGAAGAGAC CAGCAAATGT GCACAACCAA AGGTTCCACC AAAAGTTGTT 
TCTTCTAGCC AGTCAGAAGA AACTTCTGAA ATAGCCATTA ACAGGGATTA TGCCAAGAGT 
TCTGAGGGGC AAGAGAGCAA AAGGGCTTTG GCAGTGTCTG GACAAAATCC TGCTGATGAA 
AACGGCTTCC CTGATAAAGT TACAACAGAA CGTCGTCAAA GATTAGGGAC TTTTGCAGAA 
TATGAGGCAT CTTGGAAAGA ACAGAAAACA CAGCTGGAGA GAAAAAATTC TGGCAGATGT 
CATTCGGCTG ACAACATCCT TGATGCTGAT CTTGAGCAGA ATCCTAAAGC TCAGTATATT 
CATGAGCGGT CCAAGTCTTC TCCTACCACA GATTTTTATG CACAGGCAGC AGCCGTTGAG 
TCAAAGCAGC AGTCAGAGAG CGTGAGGAGA GACACCGAGA ATTCTAACTC GACACACTGC 
CGCAGTAGCA CAGGGGATTC TCCCACAAGA ACTGTTGAAG CTGGCGATCA GTGTGGTGCT 
ACCAATGAAC AAGAGGTTTT AACCTGCAAT ACTAAATGGA AGCCACATGA TAAATCGTTT 
CCGATCCCAG AAACATCCAA TGAATCCCAG CAGAGCAGAG CAAGATCAGG GACGCTACCA 
AATGATTATA GATTTGCTCA TGAAAATGTG AATCAAGGAA ATCGGGATAT CAGCTTTTCT 
GCTGTTCCTA TATCTGAAGC CTGCCCTGAT TTCTCCAATG CAGACAGCGA TCAGTTGCAA 
GATCACCCAT CTGTGTTTAA GAAGCGCAGT GCTGCCCCAC AAAGGCCTCC TCCGCCTAAA 
CTTGAAAACA AATATTGGCG GCAGAATGGC TCCTCATCTT CCCTTGCCAC ATCCTCAGAG 
TCATTGCTGA CAGCCCAAGC CAGGAGGGCA CAATCCTATT CCCCTAGCAG CCAAGACACA 
TTTCCTCCTC AGTCTCTTCA GAAACAAAGC CCAAGTACAT ATCCTGATAA GAATCCTAGC 
ATTCATATAT ATGATTATCA ATTATCTGTG CCACCAGAGA ATGACAGGTA TCATCTAGAA 
AAGAAATACT TTGAATCTGA ACTGTCCTCA AAGTCACACT TGCAGATTCC TGGAATGGAA 
CCGTCTCGAT CACCCTCTCC TCAGTTTGCT CCACAGAAGC TTACAGACAA GCCTCCCCTT 
CTGGTTCCAG AAGAAAACTT GTCAAGGATT GAAAGGGTGA TCGATAACAC TACAGTGAAA 
ATGGTCCCCA TAAAGATTGT TCACTCTGAA ACTCATGCTG AGAAGGAAAG CAGACACAAT 
TTGCTGAGTG CTATTGAGCC CACTGCCTTA CCAACTGGTC TTGCCAAGGA CCAACTAAAG 
ACTTTGAGCA CATCAGAACA ATCCTACTCC CGATTTTGTG CTTACACCAG GCAGGAATCT 
AGAGAGGAAG AGGAATCCAG AGTGACTGAC CTTTACTCAT GCCAGAGAAA TGCAGAAGAT 
GATAATGAAA ATGATGTATC TTCTCTTGCA CCCTCAAATG CTAAAAGCAA AGATGCAATC 
TACGCGGATT TAAAGTCAGA GGAGCTGGTG AGGGAAATTG TAGATAAAGA TAAATCTCTT 
GCGGACATTT TAGATCCAAA TGCCAAGATG AGAACTACCA TGGACTTAAT GGAGGGAATC 
TTTCCAAAAG ATGAACATCT TCTAGAAGAA GCTCAGCAGC GTAGAAAACA TCTCCCAAAA 
ATACCATCCC CAAGATCAAC AGATGACAAA AAAGATGAGC AAAATGTCCC ATCAGCTGTT 
TCTCTGACAA CCAGTTCCAC GTATTACAGC ACTTCAGCAG CCAAAGCCGA GCTCCTTATT 
AAAATGAAAG ACATGCAGGA GCAGCAGCAC ATTGCGGAGA ATTCAGAGGA TGAGCTGGGT 
CAGAACCTAT CCGAGAAAAA GCAAGAACTG ATTGACAGCA TTAGCAAGAA GCTGCAGGTC 
CTGCGAGATG CAAAGGAAAC CCTGTTAGAA GATGTCCAGT GTAACAATGC TCTGGGGGAA 
GAGGTAGAAA TCATTGTGAA AGAAGTCTGC AAACCAAATG AATTTGACAA ATTCAGGATG 
TTCATTGGGG ATCTGGAGAA AATCGTCAAT CTTCTGCTGT CACTTTCTGG GCGACTTGCT 
CGAGTGGAAA ATGCTCTTAA TAACTTGGAT GAAACCGTGT CTCCTGAAGA GCGGAAAACA 
CTGTTGGAAA AAAGGAAATT ACTTACGAGA CAGCATGAAG ACGCAAAGGA ACTGAAGGAG 
AACCTAGACC GGCGTGAGCG CACCGTTTAT GAGATTTTGG CTAATTACCT TAATGAGGAA 
AACCTTGCAG ATTATGAGCA CTTTGTGAAG ATGAAGTCGG CACTCATCCT TGAGCAGCGA 
GAGCTGGAAG ACAAAATCAA GCTGAGAGAG AGTCAGCTCA AATGCCTGAC CGATAGTCTT 
CCATTGGATC GTATCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072178.1
CDS	1..5181
Translation	MLVLGCLQCFTSAWGFASRRICHSSSATPGQHSVGLGHGYQHGAQSWAERFLLRYKGLLWFVGSRAHSGGEETNGKLQAGGCYSYWRGSLGIYFERGKEHGEPLIITKVEDGNKAADILMAGDEIVNINDQQLLGYRQEAICLVKGSHKILKMIVKRRNDISYRPHSWHSNKLIESAMDTVTPQMSNASPFWNARYRSSSSSHDLSNPWDQTSLQRNSGHFSSMGSMDSIDQTYQFGRLSSAKSNNSIDYVGSQNKRDSAYGSFSASFSTPDHTLSKTASASTENILYKNNEWDNTKLGYGKTSPSMNEVRRSADRQVLQSTSINETSKIQRTEDNTEPRYSGRSNFGPVWNIPDKKKTASPPPPPPPQRSDSYAVTKIHEKPTNLMHLDASSTQHFNVANRSQAKPDWSLEISEQQRPIRAHDRTVADTRRTSNSSYHAGLNADQGLSPYKDKYPSNLPNVSRIQASLSTNDVRFAQPAYNYHHQRQYSDESTLFQSTRTSAQHKSQQQPMKYESSVNQVPSDLTYVYHPHQFRAPATSAGFSSGKQNVENNGQNHFHVVSIKHPQGNTTHHQFKEEENYAPEVNSGRKSVQPENTVISIEIPAYSLPQESFEPSQINCEKNYLKISEKKDNYLGNNEQIINKTTGYSEEKCNDRFSQLDHSEKGSYRSSQDYSWRKEENKITPLVTPMLHSLAQEGRNRSESFPDTGNEKLSFPDAGKQSRRSDRFATTLRNEIQQRRARLQKSKSTAALTESNETETSDNWKQDSLESMSPTSEGSFSSTYRSHLQEAQARVLRATSFKRRDLDTGISDHLSLFQDRNMQISSFSGLSLDPVQPKKNTAVNSSQNVSRIGARKRFTTQQKLMSYSEPEKINEVGVEDQYNFRTENSTKRTVGSFADRWKFFEETSKCAQPKVPPKVVSSSQSEETSEIAINRDYAKSSEGQESKRALAVSGQNPADENGFPDKVTTERRQRLGTFAEYEASWKEQKTQLERKNSGRCHSADNILDADLEQNPKAQYIHERSKSSPTTDFYAQAAAVESKQQSESVRRDTENSNSTHCRSSTGDSPTRTVEAGDQCGATNEQEVLTCNTKWKPHDKSFPIPETSNESQQSRARSGTLPNDYRFAHENVNQGNRDISFSAVPISEACPDFSNADSDQLQDHPSVFKKRSAAPQRPPPPKLENKYWRQNGSSSSLATSSESLLTAQARRAQSYSPSSQDTFPPQSLQKQSPSTYPDKNPSIHIYDYQLSVPPENDRYHLEKKYFESELSSKSHLQIPGMEPSRSPSPQFAPQKLTDKPPLLVPEENLSRIERVIDNTTVKMVPIKIVHSETHAEKESRHNLLSAIEPTALPTGLAKDQLKTLSTSEQSYSRFCAYTRQESREEEESRVTDLYSCQRNAEDDNENDVSSLAPSNAKSKDAIYADLKSEELVREIVDKDKSLADILDPNAKMRTTMDLMEGIFPKDEHLLEEAQQRRKHLPKIPSPRSTDDKKDEQNVPSAVSLTTSSTYYSTSAAKAELLIKMKDMQEQQHIAENSEDELGQNLSEKKQELIDSISKKLQVLRDAKETLLEDVQCNNALGEEVEIIVKEVCKPNEFDKFRMFIGDLEKIVNLLLSLSGRLARVENALNNLDETVSPEERKTLLEKRKLLTRQHEDAKELKENLDRRERTVYEILANYLNEENLADYEHFVKMKSALILEQRELEDKIKLRESQLKCLTDSLPLDRIK

Target ORF information:

RefSeq Version	NM_001078710.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 2 (shroom2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078710.1

ORF Insert Sequence:

ATGTTGGTGT TAGGCTGCCT GCAATGTTTT ACCTCTGCCT GGGGATTTGC TTCTAGGAGG 
ATCTGTCATT CATCGTCTGC TACTCCTGGA CAGCACAGTG TTGGCTTGGG GCACGGCTAC 
CAGCATGGAG CCCAGAGCTG GGCAGAGAGG TTTCTGCTGA GATACAAGGG TTTATTATGG 
TTTGTTGGAT CAAGGGCACA CAGTGGTGGA GAGGAAACTA ATGGAAAGCT ACAGGCTGGT 
GGATGTTATT CTTACTGGAG GGGCTCCTTG GGGATTTACT TTGAAAGGGG CAAAGAACAT 
GGAGAGCCTC TTATCATTAC CAAGGTGGAA GATGGAAACA AAGCTGCTGA CATATTAATG 
GCTGGAGATG AAATTGTCAA CATAAATGAT CAGCAGCTTT TAGGTTATCG TCAGGAGGCA 
ATATGTCTGG TTAAAGGATC ACACAAGATT CTAAAAATGA TAGTAAAACG GAGAAATGAC 
ATCTCATACA GGCCTCATTC CTGGCACTCA AATAAGTTGA TTGAAAGTGC TATGGATACA 
GTAACTCCGC AGATGTCAAA TGCCAGCCCA TTTTGGAATG CCCGATATCG CTCCAGCTCT 
TCATCTCATG ATCTCTCAAA TCCTTGGGAC CAAACAAGCT TGCAAAGAAA TTCTGGACAT 
TTTAGCTCCA TGGGGAGTAT GGACAGTATT GACCAAACAT ATCAGTTTGG AAGACTTTCA 
TCTGCTAAAT CAAATAACAG TATAGATTAT GTAGGAAGCC AGAATAAGAG AGACTCAGCA 
TATGGTTCTT TTTCTGCAAG TTTCAGCACT CCAGATCATA CCTTATCTAA AACTGCTTCT 
GCATCAACAG AAAATATTCT ATATAAGAAC AATGAGTGGG ACAACACAAA GCTAGGTTAT 
GGCAAGACAA GTCCATCTAT GAATGAAGTC AGACGTTCTG CAGATAGACA GGTATTACAG 
TCTACTTCTA TTAATGAGAC AAGCAAAATA CAGAGGACAG AAGATAACAC TGAACCTAGA 
TATTCTGGGC GATCAAACTT TGGTCCTGTA TGGAATATTC CTGATAAAAA AAAGACAGCC 
TCACCTCCCC CTCCACCACC ACCACAGCGC AGTGATAGTT ATGCAGTCAC TAAAATCCAT 
GAGAAGCCTA CCAACCTTAT GCACTTGGAT GCTTCAAGCA CTCAGCACTT TAATGTTGCA 
AATAGGTCTC AAGCAAAACC TGACTGGAGC TTAGAAATAT CTGAACAACA AAGACCTATA 
AGAGCACATG ACAGGACTGT AGCTGATACA AGAAGAACCA GCAATTCCTC TTACCATGCT 
GGCTTAAATG CAGATCAAGG GTTGTCCCCC TATAAGGACA AATATCCAAG TAATTTGCCC 
AATGTCAGTA GAATACAGGC TTCATTATCT ACCAATGATG TCAGGTTTGC ACAACCTGCT 
TACAACTATC ATCACCAGCG GCAATACAGT GATGAAAGCA CTCTTTTTCA GAGCACAAGA 
ACATCAGCAC AGCATAAATC CCAACAACAG CCAATGAAAT ATGAATCTAG TGTAAATCAG 
GTGCCATCTG ATTTGACTTA TGTTTACCAT CCTCATCAAT TTAGAGCACC TGCTACTTCT 
GCAGGTTTTA GTAGTGGGAA ACAAAATGTA GAAAACAATG GGCAGAACCA TTTTCACGTT 
GTGAGTATAA AACATCCTCA GGGAAATACA ACACACCATC AGTTCAAGGA GGAGGAAAAC 
TATGCACCAG AGGTGAACTC TGGAAGAAAA TCTGTCCAAC CTGAAAATAC TGTCATTAGC 
ATTGAAATAC CAGCTTACAG TCTACCTCAG GAAAGTTTTG AACCTTCACA AATAAACTGT 
GAAAAGAACT ACCTAAAAAT ATCTGAGAAA AAGGATAATT ATTTAGGTAA TAATGAACAA 
ATAATCAATA AAACCACTGG ATATTCAGAA GAAAAATGTA ACGACAGATT TAGCCAGCTG 
GACCATTCTG AAAAAGGTTC ATATAGATCA AGTCAAGATT ATTCTTGGAG AAAGGAAGAA 
AATAAGATAA CACCTCTAGT AACACCAATG TTGCATTCTT TAGCTCAAGA AGGAAGAAAT 
CGATCTGAAA GTTTCCCAGA TACTGGAAAT GAAAAACTGT CTTTTCCAGA TGCGGGCAAA 
CAGTCTCGTA GAAGTGATCG GTTTGCAACG ACTCTGAGAA ATGAAATTCA GCAGAGGCGA 
GCTCGTCTGC AAAAGAGCAA AAGTACAGCT GCCTTGACCG AGTCAAACGA AACAGAGACC 
TCTGACAACT GGAAACAAGA CTCACTGGAA AGCATGAGCC CAACTTCTGA AGGTTCTTTT 
TCAAGCACCT ACAGAAGTCA TCTGCAAGAA GCCCAGGCCC GTGTTTTAAG AGCTACTTCC 
TTTAAAAGAA GAGACCTTGA TACAGGTATT TCTGATCATC TTTCACTGTT TCAAGATCGA 
AATATGCAGA TCTCAAGCTT TTCAGGCCTA TCTTTAGATC CTGTTCAACC TAAAAAAAAC 
ACAGCTGTTA ACAGCTCACA AAATGTATCT CGTATTGGAG CAAGAAAGAG GTTCACAACT 
CAGCAGAAGC TGATGTCCTA CTCAGAGCCT GAGAAAATTA ATGAAGTTGG TGTTGAAGAC 
CAATACAATT TCCGTACTGA AAATTCTACT AAAAGAACTG TGGGATCTTT TGCAGACAGA 
TGGAAATTCT TTGAAGAGAC CAGCAAATGT GCACAACCAA AGGTTCCACC AAAAGTTGTT 
TCTTCTAGCC AGTCAGAAGA AACTTCTGAA ATAGCCATTA ACAGGGATTA TGCCAAGAGT 
TCTGAGGGGC AAGAGAGCAA AAGGGCTTTG GCAGTGTCTG GACAAAATCC TGCTGATGAA 
AACGGCTTCC CTGATAAAGT TACAACAGAA CGTCGTCAAA GATTAGGGAC TTTTGCAGAA 
TATGAGGCAT CTTGGAAAGA ACAGAAAACA CAGCTGGAGA GAAAAAATTC TGGCAGATGT 
CATTCGGCTG ACAACATCCT TGATGCTGAT CTTGAGCAGA ATCCTAAAGC TCAGTATATT 
CATGAGCGGT CCAAGTCTTC TCCTACCACA GATTTTTATG CACAGGCAGC AGCCGTTGAG 
TCAAAGCAGC AGTCAGAGAG CGTGAGGAGA GACACCGAGA ATTCTAACTC GACACACTGC 
CGCAGTAGCA CAGGGGATTC TCCCACAAGA ACTGTTGAAG CTGGCGATCA GTGTGGTGCT 
ACCAATGAAC AAGAGGTTTT AACCTGCAAT ACTAAATGGA AGCCACATGA TAAATCGTTT 
CCGATCCCAG AAACATCCAA TGAATCCCAG CAGAGCAGAG CAAGATCAGG GACGCTACCA 
AATGATTATA GATTTGCTCA TGAAAATGTG AATCAAGGAA ATCGGGATAT CAGCTTTTCT 
GCTGTTCCTA TATCTGAAGC CTGCCCTGAT TTCTCCAATG CAGACAGCGA TCAGTTGCAA 
GATCACCCAT CTGTGTTTAA GAAGCGCAGT GCTGCCCCAC AAAGGCCTCC TCCGCCTAAA 
CTTGAAAACA AATATTGGCG GCAGAATGGC TCCTCATCTT CCCTTGCCAC ATCCTCAGAG 
TCATTGCTGA CAGCCCAAGC CAGGAGGGCA CAATCCTATT CCCCTAGCAG CCAAGACACA 
TTTCCTCCTC AGTCTCTTCA GAAACAAAGC CCAAGTACAT ATCCTGATAA GAATCCTAGC 
ATTCATATAT ATGATTATCA ATTATCTGTG CCACCAGAGA ATGACAGGTA TCATCTAGAA 
AAGAAATACT TTGAATCTGA ACTGTCCTCA AAGTCACACT TGCAGATTCC TGGAATGGAA 
CCGTCTCGAT CACCCTCTCC TCAGTTTGCT CCACAGAAGC TTACAGACAA GCCTCCCCTT 
CTGGTTCCAG AAGAAAACTT GTCAAGGATT GAAAGGGTGA TCGATAACAC TACAGTGAAA 
ATGGTCCCCA TAAAGATTGT TCACTCTGAA ACTCATGCTG AGAAGGAAAG CAGACACAAT 
TTGCTGAGTG CTATTGAGCC CACTGCCTTA CCAACTGGTC TTGCCAAGGA CCAACTAAAG 
ACTTTGAGCA CATCAGAACA ATCCTACTCC CGATTTTGTG CTTACACCAG GCAGGAATCT 
AGAGAGGAAG AGGAATCCAG AGTGACTGAC CTTTACTCAT GCCAGAGAAA TGCAGAAGAT 
GATAATGAAA ATGATGTATC TTCTCTTGCA CCCTCAAATG CTAAAAGCAA AGATGCAATC 
TACGCGGATT TAAAGTCAGA GGAGCTGGTG AGGGAAATTG TAGATAAAGA TAAATCTCTT 
GCGGACATTT TAGATCCAAA TGCCAAGATG AGAACTACCA TGGACTTAAT GGAGGGAATC 
TTTCCAAAAG ATGAACATCT TCTAGAAGAA GCTCAGCAGC GTAGAAAACA TCTCCCAAAA 
ATACCATCCC CAAGATCAAC AGATGACAAA AAAGATGAGC AAAATGTCCC ATCAGCTGTT 
TCTCTGACAA CCAGTTCCAC GTATTACAGC ACTTCAGCAG CCAAAGCCGA GCTCCTTATT 
AAAATGAAAG ACATGCAGGA GCAGCAGCAC ATTGCGGAGA ATTCAGAGGA TGAGCTGGGT 
CAGAACCTAT CCGAGAAAAA GCAAGAACTG ATTGACAGCA TTAGCAAGAA GCTGCAGGTC 
CTGCGAGATG CAAAGGAAAC CCTGTTAGAA GATGTCCAGT GTAACAATGC TCTGGGGGAA 
GAGGTAGAAA TCATTGTGAA AGAAGTCTGC AAACCAAATG AATTTGACAA ATTCAGGATG 
TTCATTGGGG ATCTGGAGAA AATCGTCAAT CTTCTGCTGT CACTTTCTGG GCGACTTGCT 
CGAGTGGAAA ATGCTCTTAA TAACTTGGAT GAAACCGTGT CTCCTGAAGA GCGGAAAACA 
CTGTTGGAAA AAAGGAAATT ACTTACGAGA CAGCATGAAG ACGCAAAGGA ACTGAAGGAG 
AACCTAGACC GGCGTGAGCG CACCGTTTAT GAGATTTTGG CTAATTACCT TAATGAGGAA 
AACCTTGCAG ATTATGAGCA CTTTGTGAAG ATGAAGTCGG CACTCATCCT TGAGCAGCGA 
GAGCTGGAAG ACAAAATCAA GCTGAGAGAG AGTCAGCTCA AATGCCTGAC CGATAGTCTT 
CCATTGGATC GTATCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shroom2 ( XM_012957215.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812669.1 Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X2, mRNA.

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CloneID	OXa28992
Clone ID Related Accession (Same CDS sequence)	XM_012957215.2
Accession Version	XM_012957215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Shroom2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGA TGGTGGATGT TATAAAACTG GAAATGCCTT CAGATAAAGA TGTTCTTGTG 
GCCAGGAAGT TTCTTATGAA GATCTTGAGA AGTTCAATGA GGAGAAATGA CATCTCATAC 
AGGCCTCATT CCTGGCACTC AAATAAGTTG ATTGAAAGTG CTATGGATAC AGTAACTCCG 
CAGATGTCAA ATGCCAGCCC ATTTTGGAAT GCCCGATATC GCTCCAGCTC TTCATCTCAT 
GATCTCTCAA ATCCTTGGGA CCAAACAAGC TTGCAAAGAA ATTCTGGACA TTTTAGCTCC 
ATGGGGAGTA TGGACAGTAT TGACCAAACA TATCAGTTTG GAAGACTTTC ATCTGCTAAA 
TCAAATAACA GTATAGATTA TGTAGGAAGC CAGAATAAGA GAGACTCAGC ATATGGTTCT 
TTTTCTGCAA GTTTCAGCAC TCCAGATCAT ACCTTATCTA AAACTGCTTC TGCATCAACA 
GAAAATATTC TATATAAGAA CAATGAGTGG GACAACACAA AGCTAGGTTA TGGCAAGACA 
AGTCCATCTA TGAATGAAGT CAGACGTTCT GCAGATAGAC AGGTATTACA GTCTACTTCT 
ATTAATGAGA CAAGCAAAAT ACAGAGGACA GAAGATAACA CTGAACCTAG ATATTCTGGG 
CGATCAAACT TTGGTCCTGT ATGGAATATT CCTGATAAAA AAAAGACAGC CTCACCTCCC 
CCTCCACCAC CACCACAGCG CAGTGATAGT TATGCAGTCA CTAAAATCCA TGAGAAGCCT 
ACCAACCTTA TGCACTTGGA TGCTTCAAGC ACTCAGCACT TTAATGTTGC AAATAGGTCT 
CAAGCAAAAC CTGACTGGAG CTTAGAAATA TCTGAACAAC AAAGACCTAT AAGAGCACAT 
GACAGGACTG TAGCTGATAC AAGAAGAACC AGCAATTCCT CTTACCATGC TGGCTTAAAT 
GCAGATCAAG GGTTGTCCCC CTATAAGGAC AAATATCCAA GTAATTTGCC CAATGTCAGT 
AGAATACAGG CTTCATTATC TACCAATGAT GTCAGGTTTG CACAACCTGC TTACAACTAT 
CATCACCAGC GGCAATACAG TGATGAAAGC ACTCTTTTTC AGAGCACAAG AACATCAGCA 
CAGCATAAAT CCCAACAACA GCCAATGAAA TATGAATCTA GTGTAAATCA GGTGCCATCT 
GATTTGACTT ATGTTTACCA TCCTCATCAA TTTAGAGCAC CTGCTACTTC TGCAGGTTTT 
AGTAGTGGGA AACAAAATGT AGAAAACAAT GGGCAGAACC ATTTTCACGT TGTGAGTATA 
AAACATCCTC AGGGAAATAC AACACACCAT CAGTTCAAGG AGGAGGAAAA CTATGCACCA 
GAGGTGAACT CTGGAAGAAA ATCTGTCCAA CCTGAAAATA CTGTCATTAG CATTGAAATA 
CCAGCTTACA GTCTACCTCA GGAAAGTTTT GAACCTTCAC AAATAAACTG TGAAAAGAAC 
TACCTAAAAA TATCTGAGAA AAAGGATAAT TATTTAGGTA ATAATGAACA AATAATCAAT 
AAAACCACTG GATATTCAGA AGAAAAATGT AACGACAGAT TTAGCCAGCT GGACCATTCT 
GAAAAAGGTT CATATAGATC AAGTCAAGAT TATTCTTGGA GAAAGGAAGA AAATAAGATA 
ACACCTCTAG TAACACCAAT GTTGCATTCT TTAGCTCAAG AAGGAAGAAA TCGATCTGAA 
AGTTTCCCAG ATACTGGAAA TGAAAAACTG TCTTTTCCAG ATGCGGGCAA ACAGTCTCGT 
AGAAGTGATC GGTTTGCAAC GACTCTGAGA AATGAAATTC AGCAGAGGCG AGCTCGTCTG 
CAAAAGAGCA AAAGTACAGC TGCCTTGACC GAGTCAAACG AAACAGAGAC CTCTGACAAC 
TGGAAACAAG ACTCACTGGA AAGCATGAGC CCAACTTCTG AAGGTTCTTT TTCAAGCACC 
TACAGAAGTC ATCTGCAAGA AGCCCAGGCC CGTGTTTTAA GAGCTACTTC CTTTAAAAGA 
AGAGACCTTG ATACAGGTAT TTCTGATCAT CTTTCACTGT TTCAAGATCG AAATATGCAG 
ATCTCAAGCT TTTCAGGCCT ATCTTTAGAT CCTGTTCAAC CTAAAAAAAA CACAGCTGTT 
AACAGCTCAC AAAATGTATC TCGTATTGGA GCAAGAAAGA GGTTCACAAC TCAGCAGAAG 
CTGATGTCCT ACTCAGAGCC TGAGAAAATT AATGAAGTTG GTGTTGAAGA CCAATACAAT 
TTCCGTACTG AAAATTCTAC TAAAAGAACT GTGGGATCTT TTGCAGACAG ATGGAAATTC 
TTTGAAGAGA CCAGCAAATG TGCACAACCA AAGGTTCCAC CAAAAGTTGT TTCTTCTAGC 
CAGTCAGAAG AAACTTCTGA AATAGCCATT AACAGGGATT ATGCCAAGAG TTCTGAGGGG 
CAAGAGAGCA AAAGGGCTTT GGCAGTGTCT GGACAAAATC CTGCTGATGA AAACGGCTTC 
CCTGATAAAG TTACAACAGA ACGTCGTCAA AGATTAGGGA CTTTTGCAGA ATATGAGGCA 
TCTTGGAAAG AACAGAAAAC ACAGCTGGAG AGAAAAAATT CTGGCAGATG TCATTCGGCT 
GACAACATCC TTGATGCTGA TCTTGAGCAG AATCCTAAAG CTCAGTATAT TCATGAGCGG 
TCCAAGTCTT CTCCTACCAC AGATTTTTAT GCACAGGCAG CAGCCGTTGA GTCAAAGCAG 
CAGTCAGAGA GCGTGAGGAG AGACACCGAG AATTCTAACT CGACACACTG CCGCAGTAGC 
ACAGGGGATT CTCCCACAAG AACTGTTGAA GCTGGCGATC AGTGTGGTGC TACCAATGAA 
CAAGAGGTTT TAACCTGCAA TACTAAATGG AAGCCACATG ATAAATCGTT TCCGATCCCA 
GAAACATCCA ATGAATCCCA GCAGAGCAGA GCAAGATCAG GGACGCTACC AAATGATTAT 
AGATTTGCTC ATGAAAATGT GAATCAAGGA AATCGGGATA TCAGCTTTTC TGCTGTTCCT 
ATATCTGAAG CCTGCCCTGA TTTCTCCAAT GCAGACAGCG ATCAGTTGCA AGATCACCCA 
TCTGTGTTTA AGAAGCGCAG TGCTGCCCCA CAAAGGCCTC CTCCGCCTAA ACTTGAAAAC 
AAATATTGGC GGCAGAATGG CTCCTCATCT TCCCTTGCCA CATCCTCAGA GTCATTGCTG 
ACAGCCCAAG CCAGGAGGGC ACAATCCTAT TCCCCTAGCA GCCAAGACAC ATTTCCTCCT 
CAGTCTCTTC AGAAACAAAG CCCAAGTACA TATCCTGATA AGAATCCTAG CATTCATATA 
TATGATTATC AATTATCTGT GCCACCAGAG AATGACAGGT ATCATCTAGA AAAGAAATAC 
TTTGAATCTG AACTGTCCTC AAAGTCACAC TTGCAGATTC CTGGAATGGA ACCGTCTCGA 
TCACCCTCTC CTCAGTTTGC TCCACAGAAG CTTACAGACA AGCCTCCCCT TCTGGTTCCA 
GAAGAAAACT TGTCAAGGAT TGAAAGGGTG ATCGATAACA CTACAGTGAA AATGGTCCCC 
ATAAAGATTG TTCACTCTGA AACTCATGCT GAGAAGGAAA GCAGACACAA TTTGCTGAGT 
GCTATTGAGC CCACTGCCTT ACCAACTGGT CTTGCCAAGG ACCAACTAAA GACTTTGAGC 
ACATCAGAAC AATCCTACTC CCGATTTTGT GCTTACACCA GGCAGGAATC TAGAGAGGAA 
GAGGAATCCA GAGTGACTGA CCTTTACTCA TGCCAGAGAA ATGCAGAAGA TGATAATGAA 
AATGATGTAT CTTCTCTTGC ACCCTCAAAT GCTAAAAGCA AAGATGCAAT CTACGCGGAT 
TTAAAGTCAG AGGAGCTGGT GAGGGAAATT GTAGATAAAG ATAAATCTCT TGCGGACATT 
TTAGATCCAA ATGCCAAGAT GAGAACTACC ATGGACTTAA TGGAGGGAAT CTTTCCAAAA 
GATGAACATC TTCTAGAAGA AGCTCAGCAG CGTAGAAAAC ATCTCCCAAA AATACCATCC 
CCAAGATCAA CAGATGACAA AAAAGATGAG CAAAATGTCC CATCAGCTGT TTCTCTGACA 
ACCAGTTCCA CGTATTACAG CACTTCAGCA GCCAAAGCCG AGCTCCTTAT TAAAATGAAA 
GACATGCAGG AGCAGCAGCA CATTGCGGAG AATTCAGAGG ATGAGCTGGG TCAGAACCTA 
TCCGAGAAAA AGCAAGAACT GATTGACAGC ATTAGCAAGA AGCTGCAGGT CCTGCGAGAT 
GCAAAGGAAA CCCTGTTAGA AGATGTCCAG TGTAACAATG CTCTGGGGGA AGAGGTAGAA 
ATCATTGTGA AAGAAGTCTG CAAACCAAAT GAATTTGACA AATTCAGGAT GTTCATTGGG 
GATCTGGAGA AAATCGTCAA TCTTCTGCTG TCACTTTCTG GGCGACTTGC TCGAGTGGAA 
AATGCTCTTA ATAACTTGGA TGAAACCGTG TCTCCTGAAG AGCGGAAAAC ACTGTTGGAA 
AAAAGGAAAT TACTTACGAG ACAGCATGAA GACGCAAAGG AACTGAAGGA GAACCTAGAC 
CGGCGTGAGC GCACCGTTTA TGAGATTTTG GCTAATTACC TTAATGAGGA AAACCTTGCA 
GATTATGAGC ACTTTGTGAA GATGAAGTCG GCACTCATCC TTGAGCAGCG AGAGCTGGAA 
GACAAAATCA AGCTTGGAGA GGAGCAGCTC AAATGCCTGA CCGATAGTCT TCCATTGGAT 
CGTATCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812669.1
CDS	146..4957
Translation	MNMMVDVIKLEMPSDKDVLVARKFLMKILRSSMRRNDISYRPHSWHSNKLIESAMDTVTPQMSNASPFWNARYRSSSSSHDLSNPWDQTSLQRNSGHFSSMGSMDSIDQTYQFGRLSSAKSNNSIDYVGSQNKRDSAYGSFSASFSTPDHTLSKTASASTENILYKNNEWDNTKLGYGKTSPSMNEVRRSADRQVLQSTSINETSKIQRTEDNTEPRYSGRSNFGPVWNIPDKKKTASPPPPPPPQRSDSYAVTKIHEKPTNLMHLDASSTQHFNVANRSQAKPDWSLEISEQQRPIRAHDRTVADTRRTSNSSYHAGLNADQGLSPYKDKYPSNLPNVSRIQASLSTNDVRFAQPAYNYHHQRQYSDESTLFQSTRTSAQHKSQQQPMKYESSVNQVPSDLTYVYHPHQFRAPATSAGFSSGKQNVENNGQNHFHVVSIKHPQGNTTHHQFKEEENYAPEVNSGRKSVQPENTVISIEIPAYSLPQESFEPSQINCEKNYLKISEKKDNYLGNNEQIINKTTGYSEEKCNDRFSQLDHSEKGSYRSSQDYSWRKEENKITPLVTPMLHSLAQEGRNRSESFPDTGNEKLSFPDAGKQSRRSDRFATTLRNEIQQRRARLQKSKSTAALTESNETETSDNWKQDSLESMSPTSEGSFSSTYRSHLQEAQARVLRATSFKRRDLDTGISDHLSLFQDRNMQISSFSGLSLDPVQPKKNTAVNSSQNVSRIGARKRFTTQQKLMSYSEPEKINEVGVEDQYNFRTENSTKRTVGSFADRWKFFEETSKCAQPKVPPKVVSSSQSEETSEIAINRDYAKSSEGQESKRALAVSGQNPADENGFPDKVTTERRQRLGTFAEYEASWKEQKTQLERKNSGRCHSADNILDADLEQNPKAQYIHERSKSSPTTDFYAQAAAVESKQQSESVRRDTENSNSTHCRSSTGDSPTRTVEAGDQCGATNEQEVLTCNTKWKPHDKSFPIPETSNESQQSRARSGTLPNDYRFAHENVNQGNRDISFSAVPISEACPDFSNADSDQLQDHPSVFKKRSAAPQRPPPPKLENKYWRQNGSSSSLATSSESLLTAQARRAQSYSPSSQDTFPPQSLQKQSPSTYPDKNPSIHIYDYQLSVPPENDRYHLEKKYFESELSSKSHLQIPGMEPSRSPSPQFAPQKLTDKPPLLVPEENLSRIERVIDNTTVKMVPIKIVHSETHAEKESRHNLLSAIEPTALPTGLAKDQLKTLSTSEQSYSRFCAYTRQESREEEESRVTDLYSCQRNAEDDNENDVSSLAPSNAKSKDAIYADLKSEELVREIVDKDKSLADILDPNAKMRTTMDLMEGIFPKDEHLLEEAQQRRKHLPKIPSPRSTDDKKDEQNVPSAVSLTTSSTYYSTSAAKAELLIKMKDMQEQQHIAENSEDELGQNLSEKKQELIDSISKKLQVLRDAKETLLEDVQCNNALGEEVEIIVKEVCKPNEFDKFRMFIGDLEKIVNLLLSLSGRLARVENALNNLDETVSPEERKTLLEKRKLLTRQHEDAKELKENLDRRERTVYEILANYLNEENLADYEHFVKMKSALILEQRELEDKIKLGEEQLKCLTDSLPLDRIK

Target ORF information:

RefSeq Version	XM_012957215.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis shroom family member 2 (shroom2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957215.2

ORF Insert Sequence:

ATGAACATGA TGGTGGATGT TATAAAACTG GAAATGCCTT CAGATAAAGA TGTTCTTGTG 
GCCAGGAAGT TTCTTATGAA GATCTTGAGA AGTTCAATGA GGAGAAATGA CATCTCATAC 
AGGCCTCATT CCTGGCACTC AAATAAGTTG ATTGAAAGTG CTATGGATAC AGTAACTCCG 
CAGATGTCAA ATGCCAGCCC ATTTTGGAAT GCCCGATATC GCTCCAGCTC TTCATCTCAT 
GATCTCTCAA ATCCTTGGGA CCAAACAAGC TTGCAAAGAA ATTCTGGACA TTTTAGCTCC 
ATGGGGAGTA TGGACAGTAT TGACCAAACA TATCAGTTTG GAAGACTTTC ATCTGCTAAA 
TCAAATAACA GTATAGATTA TGTAGGAAGC CAGAATAAGA GAGACTCAGC ATATGGTTCT 
TTTTCTGCAA GTTTCAGCAC TCCAGATCAT ACCTTATCTA AAACTGCTTC TGCATCAACA 
GAAAATATTC TATATAAGAA CAATGAGTGG GACAACACAA AGCTAGGTTA TGGCAAGACA 
AGTCCATCTA TGAATGAAGT CAGACGTTCT GCAGATAGAC AGGTATTACA GTCTACTTCT 
ATTAATGAGA CAAGCAAAAT ACAGAGGACA GAAGATAACA CTGAACCTAG ATATTCTGGG 
CGATCAAACT TTGGTCCTGT ATGGAATATT CCTGATAAAA AAAAGACAGC CTCACCTCCC 
CCTCCACCAC CACCACAGCG CAGTGATAGT TATGCAGTCA CTAAAATCCA TGAGAAGCCT 
ACCAACCTTA TGCACTTGGA TGCTTCAAGC ACTCAGCACT TTAATGTTGC AAATAGGTCT 
CAAGCAAAAC CTGACTGGAG CTTAGAAATA TCTGAACAAC AAAGACCTAT AAGAGCACAT 
GACAGGACTG TAGCTGATAC AAGAAGAACC AGCAATTCCT CTTACCATGC TGGCTTAAAT 
GCAGATCAAG GGTTGTCCCC CTATAAGGAC AAATATCCAA GTAATTTGCC CAATGTCAGT 
AGAATACAGG CTTCATTATC TACCAATGAT GTCAGGTTTG CACAACCTGC TTACAACTAT 
CATCACCAGC GGCAATACAG TGATGAAAGC ACTCTTTTTC AGAGCACAAG AACATCAGCA 
CAGCATAAAT CCCAACAACA GCCAATGAAA TATGAATCTA GTGTAAATCA GGTGCCATCT 
GATTTGACTT ATGTTTACCA TCCTCATCAA TTTAGAGCAC CTGCTACTTC TGCAGGTTTT 
AGTAGTGGGA AACAAAATGT AGAAAACAAT GGGCAGAACC ATTTTCACGT TGTGAGTATA 
AAACATCCTC AGGGAAATAC AACACACCAT CAGTTCAAGG AGGAGGAAAA CTATGCACCA 
GAGGTGAACT CTGGAAGAAA ATCTGTCCAA CCTGAAAATA CTGTCATTAG CATTGAAATA 
CCAGCTTACA GTCTACCTCA GGAAAGTTTT GAACCTTCAC AAATAAACTG TGAAAAGAAC 
TACCTAAAAA TATCTGAGAA AAAGGATAAT TATTTAGGTA ATAATGAACA AATAATCAAT 
AAAACCACTG GATATTCAGA AGAAAAATGT AACGACAGAT TTAGCCAGCT GGACCATTCT 
GAAAAAGGTT CATATAGATC AAGTCAAGAT TATTCTTGGA GAAAGGAAGA AAATAAGATA 
ACACCTCTAG TAACACCAAT GTTGCATTCT TTAGCTCAAG AAGGAAGAAA TCGATCTGAA 
AGTTTCCCAG ATACTGGAAA TGAAAAACTG TCTTTTCCAG ATGCGGGCAA ACAGTCTCGT 
AGAAGTGATC GGTTTGCAAC GACTCTGAGA AATGAAATTC AGCAGAGGCG AGCTCGTCTG 
CAAAAGAGCA AAAGTACAGC TGCCTTGACC GAGTCAAACG AAACAGAGAC CTCTGACAAC 
TGGAAACAAG ACTCACTGGA AAGCATGAGC CCAACTTCTG AAGGTTCTTT TTCAAGCACC 
TACAGAAGTC ATCTGCAAGA AGCCCAGGCC CGTGTTTTAA GAGCTACTTC CTTTAAAAGA 
AGAGACCTTG ATACAGGTAT TTCTGATCAT CTTTCACTGT TTCAAGATCG AAATATGCAG 
ATCTCAAGCT TTTCAGGCCT ATCTTTAGAT CCTGTTCAAC CTAAAAAAAA CACAGCTGTT 
AACAGCTCAC AAAATGTATC TCGTATTGGA GCAAGAAAGA GGTTCACAAC TCAGCAGAAG 
CTGATGTCCT ACTCAGAGCC TGAGAAAATT AATGAAGTTG GTGTTGAAGA CCAATACAAT 
TTCCGTACTG AAAATTCTAC TAAAAGAACT GTGGGATCTT TTGCAGACAG ATGGAAATTC 
TTTGAAGAGA CCAGCAAATG TGCACAACCA AAGGTTCCAC CAAAAGTTGT TTCTTCTAGC 
CAGTCAGAAG AAACTTCTGA AATAGCCATT AACAGGGATT ATGCCAAGAG TTCTGAGGGG 
CAAGAGAGCA AAAGGGCTTT GGCAGTGTCT GGACAAAATC CTGCTGATGA AAACGGCTTC 
CCTGATAAAG TTACAACAGA ACGTCGTCAA AGATTAGGGA CTTTTGCAGA ATATGAGGCA 
TCTTGGAAAG AACAGAAAAC ACAGCTGGAG AGAAAAAATT CTGGCAGATG TCATTCGGCT 
GACAACATCC TTGATGCTGA TCTTGAGCAG AATCCTAAAG CTCAGTATAT TCATGAGCGG 
TCCAAGTCTT CTCCTACCAC AGATTTTTAT GCACAGGCAG CAGCCGTTGA GTCAAAGCAG 
CAGTCAGAGA GCGTGAGGAG AGACACCGAG AATTCTAACT CGACACACTG CCGCAGTAGC 
ACAGGGGATT CTCCCACAAG AACTGTTGAA GCTGGCGATC AGTGTGGTGC TACCAATGAA 
CAAGAGGTTT TAACCTGCAA TACTAAATGG AAGCCACATG ATAAATCGTT TCCGATCCCA 
GAAACATCCA ATGAATCCCA GCAGAGCAGA GCAAGATCAG GGACGCTACC AAATGATTAT 
AGATTTGCTC ATGAAAATGT GAATCAAGGA AATCGGGATA TCAGCTTTTC TGCTGTTCCT 
ATATCTGAAG CCTGCCCTGA TTTCTCCAAT GCAGACAGCG ATCAGTTGCA AGATCACCCA 
TCTGTGTTTA AGAAGCGCAG TGCTGCCCCA CAAAGGCCTC CTCCGCCTAA ACTTGAAAAC 
AAATATTGGC GGCAGAATGG CTCCTCATCT TCCCTTGCCA CATCCTCAGA GTCATTGCTG 
ACAGCCCAAG CCAGGAGGGC ACAATCCTAT TCCCCTAGCA GCCAAGACAC ATTTCCTCCT 
CAGTCTCTTC AGAAACAAAG CCCAAGTACA TATCCTGATA AGAATCCTAG CATTCATATA 
TATGATTATC AATTATCTGT GCCACCAGAG AATGACAGGT ATCATCTAGA AAAGAAATAC 
TTTGAATCTG AACTGTCCTC AAAGTCACAC TTGCAGATTC CTGGAATGGA ACCGTCTCGA 
TCACCCTCTC CTCAGTTTGC TCCACAGAAG CTTACAGACA AGCCTCCCCT TCTGGTTCCA 
GAAGAAAACT TGTCAAGGAT TGAAAGGGTG ATCGATAACA CTACAGTGAA AATGGTCCCC 
ATAAAGATTG TTCACTCTGA AACTCATGCT GAGAAGGAAA GCAGACACAA TTTGCTGAGT 
GCTATTGAGC CCACTGCCTT ACCAACTGGT CTTGCCAAGG ACCAACTAAA GACTTTGAGC 
ACATCAGAAC AATCCTACTC CCGATTTTGT GCTTACACCA GGCAGGAATC TAGAGAGGAA 
GAGGAATCCA GAGTGACTGA CCTTTACTCA TGCCAGAGAA ATGCAGAAGA TGATAATGAA 
AATGATGTAT CTTCTCTTGC ACCCTCAAAT GCTAAAAGCA AAGATGCAAT CTACGCGGAT 
TTAAAGTCAG AGGAGCTGGT GAGGGAAATT GTAGATAAAG ATAAATCTCT TGCGGACATT 
TTAGATCCAA ATGCCAAGAT GAGAACTACC ATGGACTTAA TGGAGGGAAT CTTTCCAAAA 
GATGAACATC TTCTAGAAGA AGCTCAGCAG CGTAGAAAAC ATCTCCCAAA AATACCATCC 
CCAAGATCAA CAGATGACAA AAAAGATGAG CAAAATGTCC CATCAGCTGT TTCTCTGACA 
ACCAGTTCCA CGTATTACAG CACTTCAGCA GCCAAAGCCG AGCTCCTTAT TAAAATGAAA 
GACATGCAGG AGCAGCAGCA CATTGCGGAG AATTCAGAGG ATGAGCTGGG TCAGAACCTA 
TCCGAGAAAA AGCAAGAACT GATTGACAGC ATTAGCAAGA AGCTGCAGGT CCTGCGAGAT 
GCAAAGGAAA CCCTGTTAGA AGATGTCCAG TGTAACAATG CTCTGGGGGA AGAGGTAGAA 
ATCATTGTGA AAGAAGTCTG CAAACCAAAT GAATTTGACA AATTCAGGAT GTTCATTGGG 
GATCTGGAGA AAATCGTCAA TCTTCTGCTG TCACTTTCTG GGCGACTTGC TCGAGTGGAA 
AATGCTCTTA ATAACTTGGA TGAAACCGTG TCTCCTGAAG AGCGGAAAAC ACTGTTGGAA 
AAAAGGAAAT TACTTACGAG ACAGCATGAA GACGCAAAGG AACTGAAGGA GAACCTAGAC 
CGGCGTGAGC GCACCGTTTA TGAGATTTTG GCTAATTACC TTAATGAGGA AAACCTTGCA 
GATTATGAGC ACTTTGTGAA GATGAAGTCG GCACTCATCC TTGAGCAGCG AGAGCTGGAA 
GACAAAATCA AGCTTGGAGA GGAGCAGCTC AAATGCCTGA CCGATAGTCT TCCATTGGAT 
CGTATCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Shroom2 (APXL) regulates melanosome biogenesis and localization in the retinal pigment epithelium.
Development (Cambridge, England)133(20)4109-18(2006 Oct)
Fairbank PD,Lee C,Ellis A,Hildebrand JD,Gross JM,Wallingford JB

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shroom2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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