ccbe1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccbe1
Entrez Gene ID	555629
Full Name	collagen and calcium binding EGF domains 1
Synonyms	fof
General protein information	Preferred Names collagen and calcium binding EGF domains 1 Names collagen and calcium-binding EGF domain-containing protein 1 full of fluid protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017352782.1	XP_017208271.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
XM_017352782.2	XP_017208271.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X2
NM_001163923.1	NP_001157395.1	collagen and calcium-binding EGF domain-containing protein 1 precursor
XM_021468847.1	XP_021324522.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCBE1	NP_597716.1
Mus musculus (house mouse)	Ccbe1	NP_848908.1
	CCBE1	XP_001090495.2
Xenopus tropicalis (tropical clawed frog)	ccbe1	XP_002936721.2
Pan troglodytes (chimpanzee)	CCBE1	XP_512156.3
Canis lupus familiaris (dog)	CCBE1	XP_852585.2
Bos taurus (cattle)	CCBE1	XP_002697885.2
Rattus norvegicus (Norway rat)	Ccbe1	XP_341625.5
Gallus gallus (chicken)	CCBE1	XP_001233358.1
Danio rerio (zebrafish)	ccbe1	NP_001157395.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel perivascular cell population in the zebrafish brain.
Venero Galanternik M, Castranova D, Gore AV, Blewett NH, Jung HM, Stratman AN, Kirby MR, Iben J, Miller MF, Kawakami K, Maraia RJ, Weinstein BM
eLife6(2017 04)

Cilia Control Vascular Mural Cell Recruitment in Vertebrates.
Chen X, Gays D, Milia C, Santoro MM
Cell reports18(4)1033-1047(2017 Jan)

Utilising polymorphisms to achieve allele-specific genome editing in zebrafish.
Capon SJ, Baillie GJ, Bower NI, da Silva JA, Paterson S, Hogan BM, Simons C, Smith KA
Biology open6(1)125-131(2017 Jan)

Fast revascularization of the injured area is essential to support zebrafish heart regeneration.
Mar?n-Juez R, Marass M, Gauvrit S, Rossi A, Lai SL, Materna SC, Black BL, Stainier DY
Proceedings of the National Academy of Sciences of the United States of America113(40)11237-11242(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collagen domains of CCBE1 are crucial for the activation of VEGFC in vitro and in vivo. The EGF domains of CCBE1 are dispensable for regulation of VEGFC processing in vitro, however, they are necessary for full lymphangiogenic activity of CCBE1 in vivo.
Title: Functional Dissection of the CCBE1 Protein: A Crucial Requirement for the Collagen Repeat Domain.

In the embryo, phenotypes driven by increased Vegfc are suppressed in the absence of Ccbe1, and Vegfc-driven sprouting is enhanced by local Ccbe1 overexpression. Moreover, Vegfc- and Vegfr3-dependent Erk signaling is impaired in the absence of Ccbe1.
Title: Ccbe1 regulates Vegfc-mediated induction of Vegfr3 signaling during embryonic lymphangiogenesis.

The following ccbe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccbe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01571	NM_001163923.1 Latest version!	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa150901	XM_021468847.1 Latest version!	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa56778	XM_017352782.2 Latest version!	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
ODa56778	XM_017352782.1	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccbe1 ( NM_001163923.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001157395.1 Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), mRNA.

Price & Availability↑

CloneID	ODa01571
Clone ID Related Accession (Same CDS sequence)	NM_001163923.1
Accession Version	NM_001163923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	collagen and calcium-binding EGF domain-containing protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH445748.1 and BC154643.1. On Aug 8, 2009 this sequence version replaced XM_001923073.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCTACC CGGGCAGAGG AGCATCTCTC AGTGTGGCCG TGGCGCTCGT ACTGTTCTCA 
TCGGGAGCTC CGTGGACTTT CCGAGAGGAG AAGGAGGATG TAGACAGAGA AGTGTGTTCA 
GAAAGCAAAA TCGCAACGAC GAAGTACCCG TGCGTAAAGT CCACTGGAGA GGTGACCACA 
TGCTACAGAA AGAAGTGCTG TGAAGGTTTC AAGTTTGTCC TGGGTCAGTG TATTCCAGAA 
GATTATGATG TGTGTGCCGG CGCCCCGTGT GAACAGCAGT GTACGGATCA TTTCGGTCGG 
GTTGTTTGCA CCTGCTATGA CGGGTATCGA TATGACCGGG AACGACACAG AAACAGGGAG 
AAACCATATT GCCTGGACAT TGATGAATGT GCCAACAATA ACGAGACTGT GTGCTCACAG 
ATGTGTGTGA ACACTCCCGG CAGCTACCGG TGCGACTGCC ACAGCGGCTT CTACCTGGAG 
GATGATGGGA AGACATGCAC CAAGGGCGAG AGAGCTCCAC TCTTTGAAAA ATCAGACAAC 
GTCATGAAAG AAGGCACGTG TTCGGCCACA TGCGAGGACT TCCATCAGAT GAAGATGACT 
GTTCTCCAAC TCAAACAGAA GATGTCCTTA TTGTCGAGCA ACACCGAGAT CAATAAGCAG 
ATGACCAATG AAAAAATGAT GATGACGACA AATTCATTTT TACCTGGACC TCCAGGGCCT 
CCTGGGCCTG CAGGGACGCC TGGAGCCAAA GGATCCTCTG GCTCTCCTGG ACAGATGGGG 
CCACCAGGCT TACCTGGTCC CAGAGGTGAC ATGGGACCCA TTGGACCCTC CCCTGACCTG 
TCGCATATCA AACAGGGTCG ACGTGGCCCA GTGGGTCCTC CTGGGGCTCC AGGGAGAGAT 
GGCATGAAGG GAGAGCGAGG GTTTCCCGGC CCTTCCGGAC CTCCAGGCCC ACCAGGATCC 
TTTGACTTCC TGTTACTCAT GATGGCTGAC ATTCGTAACG ACATCGCTGA GCTGCAGAGT 
AAAGTCTTCA GCAGACCGTT ACATTCTTCA TTTGAGGATT TCCCATCTGC CCCGGACAGC 
TGGAGAGACA CACCGGAGAA CCTGGACTTC GGATCCGGCG AAGACTACAA ATCCCAGAGC 
CCTCCCAAGA GCAGCAGGAA GAGGAAATTA CCTCGGAACT TAAAAAATCC CGATTGGCCG 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157395.1
CDS	130..1335
Translation	MIYPGRGASLSVAVALVLFSSGAPWTFREEKEDVDREVCSESKIATTKYPCVKSTGEVTTCYRKKCCEGFKFVLGQCIPEDYDVCAGAPCEQQCTDHFGRVVCTCYDGYRYDRERHRNREKPYCLDIDECANNNETVCSQMCVNTPGSYRCDCHSGFYLEDDGKTCTKGERAPLFEKSDNVMKEGTCSATCEDFHQMKMTVLQLKQKMSLLSSNTEINKQMTNEKMMMTTNSFLPGPPGPPGPAGTPGAKGSSGSPGQMGPPGLPGPRGDMGPIGPSPDLSHIKQGRRGPVGPPGAPGRDGMKGERGFPGPSGPPGPPGSFDFLLLMMADIRNDIAELQSKVFSRPLHSSFEDFPSAPDSWRDTPENLDFGSGEDYKSQSPPKSSRKRKLPRNLKNPDWPV

Target ORF information:

RefSeq Version	NM_001163923.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163923.1

ORF Insert Sequence:

ATGATCTACC CGGGCAGAGG AGCATCTCTC AGTGTGGCCG TGGCGCTCGT ACTGTTCTCA 
TCGGGAGCTC CGTGGACTTT CCGAGAGGAG AAGGAGGATG TAGACAGAGA AGTGTGTTCA 
GAAAGCAAAA TCGCAACGAC GAAGTACCCG TGCGTAAAGT CCACTGGAGA GGTGACCACA 
TGCTACAGAA AGAAGTGCTG TGAAGGTTTC AAGTTTGTCC TGGGTCAGTG TATTCCAGAA 
GATTATGATG TGTGTGCCGG CGCCCCGTGT GAACAGCAGT GTACGGATCA TTTCGGTCGG 
GTTGTTTGCA CCTGCTATGA CGGGTATCGA TATGACCGGG AACGACACAG AAACAGGGAG 
AAACCATATT GCCTGGACAT TGATGAATGT GCCAACAATA ACGAGACTGT GTGCTCACAG 
ATGTGTGTGA ACACTCCCGG CAGCTACCGG TGCGACTGCC ACAGCGGCTT CTACCTGGAG 
GATGATGGGA AGACATGCAC CAAGGGCGAG AGAGCTCCAC TCTTTGAAAA ATCAGACAAC 
GTCATGAAAG AAGGCACGTG TTCGGCCACA TGCGAGGACT TCCATCAGAT GAAGATGACT 
GTTCTCCAAC TCAAACAGAA GATGTCCTTA TTGTCGAGCA ACACCGAGAT CAATAAGCAG 
ATGACCAATG AAAAAATGAT GATGACGACA AATTCATTTT TACCTGGACC TCCAGGGCCT 
CCTGGGCCTG CAGGGACGCC TGGAGCCAAA GGATCCTCTG GCTCTCCTGG ACAGATGGGG 
CCACCAGGCT TACCTGGTCC CAGAGGTGAC ATGGGACCCA TTGGACCCTC CCCTGACCTG 
TCGCATATCA AACAGGGTCG ACGTGGCCCA GTGGGTCCTC CTGGGGCTCC AGGGAGAGAT 
GGCATGAAGG GAGAGCGAGG GTTTCCCGGC CCTTCCGGAC CTCCAGGCCC ACCAGGATCC 
TTTGACTTCC TGTTACTCAT GATGGCTGAC ATTCGTAACG ACATCGCTGA GCTGCAGAGT 
AAAGTCTTCA GCAGACCGTT ACATTCTTCA TTTGAGGATT TCCCATCTGC CCCGGACAGC 
TGGAGAGACA CACCGGAGAA CCTGGACTTC GGATCCGGCG AAGACTACAA ATCCCAGAGC 
CCTCCCAAGA GCAGCAGGAA GAGGAAATTA CCTCGGAACT TAAAAAATCC CGATTGGCCG 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccbe1 ( XM_021468847.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324522.1 Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150901
Clone ID Related Accession (Same CDS sequence)	XM_021468847.1
Accession Version	XM_021468847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTGG TTTTAGCGGA CCGCAAGTTA AAGTTTGGGA ACCAGAACCG CTGGTTTATA 
ATAAAGAAGT GCTGTGAAGG TTTCAAGTTT GTCCTGGGTC AGTGTATTCC AGAAGATTAT 
GATGTGTGTG CCGGCGCCCC GTGTGAACAG CAGTGTACGG ATCATTTCGG TCGGGTTGTT 
TGCACCTGCT ATGACGGGTA TCGATATGAC CGGGAACGAC ACAGAAACAG GGAGAAACCA 
TATTGCCTGG ACATTGATGA ATGTGCCAAC AATAACGAGA CTGTGTGCTC ACAGATGTGT 
GTGAACACTC CCGGCAGCTA CCGGTGCGAC TGCCACAGCG GCTTCTACCT GGAGGATGAT 
GGGAAGACAT GCACCAAGGG CGAGAGAGCT CCACTCTTTG AAAAATCAGA CAACGTCATG 
AAAGAAGGCA CGTGTTCGGC CACATGCGAG GACTTCCATC AGATGAAGAT GACTGTTCTC 
CAACTCAAAC AGAAGATGTC CTTATTGTCG AGCAACACCG AGATCAATAA GCAGATGACC 
AATGAAAAAA TGATGATGAC GACAAATTCA TTTTTACCTG GACCTCCAGG GCCTCCTGGG 
CCTGCAGGGA CGCCTGGAGC CAAAGGATCC TCTGGCTCTC CTGGACAGAT GGGGCCACCA 
GGCTTACCTG GTCCCAGAGG TGACATGGGA CCCATTGGAC CCTCCCCTGA CCTGTCGCAT 
ATCAAACAGG GTCGACGTGG CCCAGTGGGT CCTCCTGGGG CTCCAGGGAG AGATGGCATG 
AAGGGAGAGC GAGGGTTTCC CGGCCCTTCC GGACCTCCAG GCCCACCAGG ATCCTTTGAC 
TTCCTGTTAC TCATGATGGC TGACATTCGT AACGACATCG CTGAGCTGCA GAGTAAAGTC 
TTCAGCAGAC CGTTACATTC TTCATTTGAG GATTTCCCAT CTGCCCCGGA CAGCTGGAGA 
GACACACCGG AGAACCTGGA CTTCGGATCC GGCGAAGACT ACAAATCCCA GAGCCCTCCC 
AAGAGCAGCA GGAAGAGGAA ATTACCTCGG AACTTAAAAA ATCCCGATTG GCCGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324522.1
CDS	93..1172
Translation	MCLVLADRKLKFGNQNRWFIIKKCCEGFKFVLGQCIPEDYDVCAGAPCEQQCTDHFGRVVCTCYDGYRYDRERHRNREKPYCLDIDECANNNETVCSQMCVNTPGSYRCDCHSGFYLEDDGKTCTKGERAPLFEKSDNVMKEGTCSATCEDFHQMKMTVLQLKQKMSLLSSNTEINKQMTNEKMMMTTNSFLPGPPGPPGPAGTPGAKGSSGSPGQMGPPGLPGPRGDMGPIGPSPDLSHIKQGRRGPVGPPGAPGRDGMKGERGFPGPSGPPGPPGSFDFLLLMMADIRNDIAELQSKVFSRPLHSSFEDFPSAPDSWRDTPENLDFGSGEDYKSQSPPKSSRKRKLPRNLKNPDWPV

Target ORF information:

RefSeq Version	XM_021468847.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468847.1

ORF Insert Sequence:

ATGTGTTTGG TTTTAGCGGA CCGCAAGTTA AAGTTTGGGA ACCAGAACCG CTGGTTTATA 
ATAAAGAAGT GCTGTGAAGG TTTCAAGTTT GTCCTGGGTC AGTGTATTCC AGAAGATTAT 
GATGTGTGTG CCGGCGCCCC GTGTGAACAG CAGTGTACGG ATCATTTCGG TCGGGTTGTT 
TGCACCTGCT ATGACGGGTA TCGATATGAC CGGGAACGAC ACAGAAACAG GGAGAAACCA 
TATTGCCTGG ACATTGATGA ATGTGCCAAC AATAACGAGA CTGTGTGCTC ACAGATGTGT 
GTGAACACTC CCGGCAGCTA CCGGTGCGAC TGCCACAGCG GCTTCTACCT GGAGGATGAT 
GGGAAGACAT GCACCAAGGG CGAGAGAGCT CCACTCTTTG AAAAATCAGA CAACGTCATG 
AAAGAAGGCA CGTGTTCGGC CACATGCGAG GACTTCCATC AGATGAAGAT GACTGTTCTC 
CAACTCAAAC AGAAGATGTC CTTATTGTCG AGCAACACCG AGATCAATAA GCAGATGACC 
AATGAAAAAA TGATGATGAC GACAAATTCA TTTTTACCTG GACCTCCAGG GCCTCCTGGG 
CCTGCAGGGA CGCCTGGAGC CAAAGGATCC TCTGGCTCTC CTGGACAGAT GGGGCCACCA 
GGCTTACCTG GTCCCAGAGG TGACATGGGA CCCATTGGAC CCTCCCCTGA CCTGTCGCAT 
ATCAAACAGG GTCGACGTGG CCCAGTGGGT CCTCCTGGGG CTCCAGGGAG AGATGGCATG 
AAGGGAGAGC GAGGGTTTCC CGGCCCTTCC GGACCTCCAG GCCCACCAGG ATCCTTTGAC 
TTCCTGTTAC TCATGATGGC TGACATTCGT AACGACATCG CTGAGCTGCA GAGTAAAGTC 
TTCAGCAGAC CGTTACATTC TTCATTTGAG GATTTCCCAT CTGCCCCGGA CAGCTGGAGA 
GACACACCGG AGAACCTGGA CTTCGGATCC GGCGAAGACT ACAAATCCCA GAGCCCTCCC 
AAGAGCAGCA GGAAGAGGAA ATTACCTCGG AACTTAAAAA ATCCCGATTG GCCGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccbe1 ( XM_017352782.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208271.1 Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56778
Clone ID Related Accession (Same CDS sequence)	XM_017352782.1 , XM_017352782.2
Accession Version	XM_017352782.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTACC CGGGCAGAGG AGCATCTCTC AGTGTGGCCG TGGCGCTCGT ACTGTTCTCA 
TCGGGAGCTC CGTGGACTTT CCGAGAGGAG AAGGAGGATG TAGACAGAGA AGTGTGTTCA 
GAAAGCAAAA TCGCAACGAC GAAGTACCCG TGCGTAAAGT CCACTGGAGA GGTGACCACA 
TGCTACAGAA AGAAGTGCTG TGAAGGTTTC AAGTTTGTCC TGGGTCAGTG TATTCCAGAA 
GATTATGATG TGTGTGCCGG CGCCCCGTGT GAACAGCAGT GTACGGATCA TTTCGGTCGG 
GTTGTTTGCA CCTGCTATGA CGGGTATCGA TATGACCGGG AACGACACAG AAACAGGGAG 
AAACCATATT GCCTGGACAT TGATGAATGT GCCAACAATA ACGAGACTGT GTGCTCACAG 
ATGTGTGTGA ACACTCCCGG CAGCTACCGG TGCGACTGCC ACAGCGGCTT CTACCTGGAG 
GATGATGGGA AGACATGCAC CAAGGGCGAG AGAGCTCCAC TCTTTGAAAA ATCAGACAAC 
GTCATGAAAG AAGGCACGTG TTCGGCCACA TGCGAGGACT TCCATCAGAT GAAGATGACT 
GTTCTCCAAC TCAAACAGAA GATGTCCTTA TTGTCGAGCA ACACCGAGAT CAATAAGCAG 
ATGACCAATG AAAAAATGAT GATGACGACA AATTCATTTT TACCTGGACC TCCAGGGCCT 
CCTGGGCCTG CAGGGACGCC TGGAGCCAAA GGATCCTCTG GCTCTCCTGG ACAGATGGGG 
CCACCAGGCT TACCTGGTCC CAGAGGTGAC ATGGGACCCA TTGGACCCTC CCCTGACCTG 
TCGCATATCA AACAGGGTCG ACGTGGCCCA GTGGGTCCTC CTGGGGCTCC AGGGAGAGAT 
GGCATGAAGG TACGGGAGAG CGAGGGTTTC CCGGCCCTTC CGGACCTCCA GGCCCACCAG 
GATCCTTTGA CTTCCTGTTA CTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208271.1
CDS	125..1111
Translation	MIYPGRGASLSVAVALVLFSSGAPWTFREEKEDVDREVCSESKIATTKYPCVKSTGEVTTCYRKKCCEGFKFVLGQCIPEDYDVCAGAPCEQQCTDHFGRVVCTCYDGYRYDRERHRNREKPYCLDIDECANNNETVCSQMCVNTPGSYRCDCHSGFYLEDDGKTCTKGERAPLFEKSDNVMKEGTCSATCEDFHQMKMTVLQLKQKMSLLSSNTEINKQMTNEKMMMTTNSFLPGPPGPPGPAGTPGAKGSSGSPGQMGPPGLPGPRGDMGPIGPSPDLSHIKQGRRGPVGPPGAPGRDGMKVRESEGFPALPDLQAHQDPLTSCYS

Target ORF information:

RefSeq Version	XM_017352782.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352782.1

ORF Insert Sequence:

ATGATCTACC CGGGCAGAGG AGCATCTCTC AGTGTGGCCG TGGCGCTCGT ACTGTTCTCA 
TCGGGAGCTC CGTGGACTTT CCGAGAGGAG AAGGAGGATG TAGACAGAGA AGTGTGTTCA 
GAAAGCAAAA TCGCAACGAC GAAGTACCCG TGCGTAAAGT CCACTGGAGA GGTGACCACA 
TGCTACAGAA AGAAGTGCTG TGAAGGTTTC AAGTTTGTCC TGGGTCAGTG TATTCCAGAA 
GATTATGATG TGTGTGCCGG CGCCCCGTGT GAACAGCAGT GTACGGATCA TTTCGGTCGG 
GTTGTTTGCA CCTGCTATGA CGGGTATCGA TATGACCGGG AACGACACAG AAACAGGGAG 
AAACCATATT GCCTGGACAT TGATGAATGT GCCAACAATA ACGAGACTGT GTGCTCACAG 
ATGTGTGTGA ACACTCCCGG CAGCTACCGG TGCGACTGCC ACAGCGGCTT CTACCTGGAG 
GATGATGGGA AGACATGCAC CAAGGGCGAG AGAGCTCCAC TCTTTGAAAA ATCAGACAAC 
GTCATGAAAG AAGGCACGTG TTCGGCCACA TGCGAGGACT TCCATCAGAT GAAGATGACT 
GTTCTCCAAC TCAAACAGAA GATGTCCTTA TTGTCGAGCA ACACCGAGAT CAATAAGCAG 
ATGACCAATG AAAAAATGAT GATGACGACA AATTCATTTT TACCTGGACC TCCAGGGCCT 
CCTGGGCCTG CAGGGACGCC TGGAGCCAAA GGATCCTCTG GCTCTCCTGG ACAGATGGGG 
CCACCAGGCT TACCTGGTCC CAGAGGTGAC ATGGGACCCA TTGGACCCTC CCCTGACCTG 
TCGCATATCA AACAGGGTCG ACGTGGCCCA GTGGGTCCTC CTGGGGCTCC AGGGAGAGAT 
GGCATGAAGG TACGGGAGAG CGAGGGTTTC CCGGCCCTTC CGGACCTCCA GGCCCACCAG 
GATCCTTTGA CTTCCTGTTA CTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccbe1 ( XM_017352782.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208271.1 Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56778
Clone ID Related Accession (Same CDS sequence)	XM_017352782.1 , XM_017352782.2
Accession Version	XM_017352782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTACC CGGGCAGAGG AGCATCTCTC AGTGTGGCCG TGGCGCTCGT ACTGTTCTCA 
TCGGGAGCTC CGTGGACTTT CCGAGAGGAG AAGGAGGATG TAGACAGAGA AGTGTGTTCA 
GAAAGCAAAA TCGCAACGAC GAAGTACCCG TGCGTAAAGT CCACTGGAGA GGTGACCACA 
TGCTACAGAA AGAAGTGCTG TGAAGGTTTC AAGTTTGTCC TGGGTCAGTG TATTCCAGAA 
GATTATGATG TGTGTGCCGG CGCCCCGTGT GAACAGCAGT GTACGGATCA TTTCGGTCGG 
GTTGTTTGCA CCTGCTATGA CGGGTATCGA TATGACCGGG AACGACACAG AAACAGGGAG 
AAACCATATT GCCTGGACAT TGATGAATGT GCCAACAATA ACGAGACTGT GTGCTCACAG 
ATGTGTGTGA ACACTCCCGG CAGCTACCGG TGCGACTGCC ACAGCGGCTT CTACCTGGAG 
GATGATGGGA AGACATGCAC CAAGGGCGAG AGAGCTCCAC TCTTTGAAAA ATCAGACAAC 
GTCATGAAAG AAGGCACGTG TTCGGCCACA TGCGAGGACT TCCATCAGAT GAAGATGACT 
GTTCTCCAAC TCAAACAGAA GATGTCCTTA TTGTCGAGCA ACACCGAGAT CAATAAGCAG 
ATGACCAATG AAAAAATGAT GATGACGACA AATTCATTTT TACCTGGACC TCCAGGGCCT 
CCTGGGCCTG CAGGGACGCC TGGAGCCAAA GGATCCTCTG GCTCTCCTGG ACAGATGGGG 
CCACCAGGCT TACCTGGTCC CAGAGGTGAC ATGGGACCCA TTGGACCCTC CCCTGACCTG 
TCGCATATCA AACAGGGTCG ACGTGGCCCA GTGGGTCCTC CTGGGGCTCC AGGGAGAGAT 
GGCATGAAGG TACGGGAGAG CGAGGGTTTC CCGGCCCTTC CGGACCTCCA GGCCCACCAG 
GATCCTTTGA CTTCCTGTTA CTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208271.1
CDS	125..1111
Translation	MIYPGRGASLSVAVALVLFSSGAPWTFREEKEDVDREVCSESKIATTKYPCVKSTGEVTTCYRKKCCEGFKFVLGQCIPEDYDVCAGAPCEQQCTDHFGRVVCTCYDGYRYDRERHRNREKPYCLDIDECANNNETVCSQMCVNTPGSYRCDCHSGFYLEDDGKTCTKGERAPLFEKSDNVMKEGTCSATCEDFHQMKMTVLQLKQKMSLLSSNTEINKQMTNEKMMMTTNSFLPGPPGPPGPAGTPGAKGSSGSPGQMGPPGLPGPRGDMGPIGPSPDLSHIKQGRRGPVGPPGAPGRDGMKVRESEGFPALPDLQAHQDPLTSCYS

Target ORF information:

RefSeq Version	XM_017352782.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen and calcium binding EGF domains 1 (ccbe1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352782.2

ORF Insert Sequence:

ATGATCTACC CGGGCAGAGG AGCATCTCTC AGTGTGGCCG TGGCGCTCGT ACTGTTCTCA 
TCGGGAGCTC CGTGGACTTT CCGAGAGGAG AAGGAGGATG TAGACAGAGA AGTGTGTTCA 
GAAAGCAAAA TCGCAACGAC GAAGTACCCG TGCGTAAAGT CCACTGGAGA GGTGACCACA 
TGCTACAGAA AGAAGTGCTG TGAAGGTTTC AAGTTTGTCC TGGGTCAGTG TATTCCAGAA 
GATTATGATG TGTGTGCCGG CGCCCCGTGT GAACAGCAGT GTACGGATCA TTTCGGTCGG 
GTTGTTTGCA CCTGCTATGA CGGGTATCGA TATGACCGGG AACGACACAG AAACAGGGAG 
AAACCATATT GCCTGGACAT TGATGAATGT GCCAACAATA ACGAGACTGT GTGCTCACAG 
ATGTGTGTGA ACACTCCCGG CAGCTACCGG TGCGACTGCC ACAGCGGCTT CTACCTGGAG 
GATGATGGGA AGACATGCAC CAAGGGCGAG AGAGCTCCAC TCTTTGAAAA ATCAGACAAC 
GTCATGAAAG AAGGCACGTG TTCGGCCACA TGCGAGGACT TCCATCAGAT GAAGATGACT 
GTTCTCCAAC TCAAACAGAA GATGTCCTTA TTGTCGAGCA ACACCGAGAT CAATAAGCAG 
ATGACCAATG AAAAAATGAT GATGACGACA AATTCATTTT TACCTGGACC TCCAGGGCCT 
CCTGGGCCTG CAGGGACGCC TGGAGCCAAA GGATCCTCTG GCTCTCCTGG ACAGATGGGG 
CCACCAGGCT TACCTGGTCC CAGAGGTGAC ATGGGACCCA TTGGACCCTC CCCTGACCTG 
TCGCATATCA AACAGGGTCG ACGTGGCCCA GTGGGTCCTC CTGGGGCTCC AGGGAGAGAT 
GGCATGAAGG TACGGGAGAG CGAGGGTTTC CCGGCCCTTC CGGACCTCCA GGCCCACCAG 
GATCCTTTGA CTTCCTGTTA CTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel perivascular cell population in the zebrafish brain.
eLife6(2017 04)
Venero Galanternik M,Castranova D,Gore AV,Blewett NH,Jung HM,Stratman AN,Kirby MR,Iben J,Miller MF,Kawakami K,Maraia RJ,Weinstein BM

Cilia Control Vascular Mural Cell Recruitment in Vertebrates.
Cell reports18(4)1033-1047(2017 Jan)
Chen X,Gays D,Milia C,Santoro MM

Utilising polymorphisms to achieve allele-specific genome editing in zebrafish.
Biology open6(1)125-131(2017 Jan)
Capon SJ,Baillie GJ,Bower NI,da Silva JA,Paterson S,Hogan BM,Simons C,Smith KA

Fast revascularization of the injured area is essential to support zebrafish heart regeneration.
Proceedings of the National Academy of Sciences of the United States of America113(40)11237-11242(2016 10)
Mar?n-Juez R,Marass M,Gauvrit S,Rossi A,Lai SL,Materna SC,Black BL,Stainier DY

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ccbe1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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