CCBE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCBE1
Entrez Gene ID	147372
Full Name	collagen and calcium binding EGF domains 1
Synonyms	HKLLS1
General protein information	Preferred Names collagen and calcium binding EGF domains 1 Names collagen and calcium-binding EGF domain-containing protein 1 full of fluid protein homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q21.32
Summary	This gene is thought to function in extracellular matrix remodeling and migration. It is predominantly expressed in the ovary, but down regulated in ovarian cancer cell lines and primary carcinomas, suggesting its role as a tumour suppressor. Mutations in this gene have been associated with Hennekam lymphangiectasia-lymphedema syndrome, a generalized lymphatic dysplasia in humans. [provided by RefSeq, Mar 2010].
Disorder MIM:	612753
Disorder Html:	Hennekam lymphangiectasia-lymphedema syndrome 1, 235510 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017025558.1	XP_016881047.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X3
NM_133459.4	NP_597716.1	collagen and calcium-binding EGF domain-containing protein 1 precursor
XM_017025557.1	XP_016881046.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
XM_024451091.1	XP_024306859.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X2
NM_133459.3	NP_597716.1	collagen and calcium-binding EGF domain-containing protein 1 precursor
XM_005266648.2	XP_005266705.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
XM_017025556.1	XP_016881045.1	collagen and calcium-binding EGF domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CCBE1	NP_597716.1
Mus musculus (house mouse)	Ccbe1	NP_848908.1
	CCBE1	XP_001090495.2
Xenopus tropicalis (tropical clawed frog)	ccbe1	XP_002936721.2
Pan troglodytes (chimpanzee)	CCBE1	XP_512156.3
Canis lupus familiaris (dog)	CCBE1	XP_852585.2
Bos taurus (cattle)	CCBE1	XP_002697885.2
Rattus norvegicus (Norway rat)	Ccbe1	XP_341625.5
Gallus gallus (chicken)	CCBE1	XP_001233358.1
Danio rerio (zebrafish)	ccbe1	NP_001157395.1

Gene Ontology
Bibliography

Related articles in PubMed

Clinical significance of CCBE1 expression in lung cancer.
Li P, Cong Z, Qiang Y, Xiong L, Tang L, Zhang Y, Wu H, Yi J, Jing H, Li D, Shen Y
Molecular medicine reports17(2)2107-2112(2018 Feb)

Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1.
Jha SK, Rauniyar K, Karpanen T, Lepp?nen VM, Brouillard P, Vikkula M, Alitalo K, Jeltsch M
Scientific reports7(1)4916(2017 Jul)

Targeting of CCBE1 by miR-330-3p in human breast cancer promotes metastasis.
Mesci A, Huang X, Taeb S, Jahangiri S, Kim Y, Fokas E, Bruce J, Leong HS, Liu SK
British journal of cancer116(10)1350-1357(2017 May)

CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib.
Tian GA, Zhu CC, Zhang XX, Zhu L, Yang XM, Jiang SH, Li RK, Tu L, Wang Y, Zhuang C, He P, Li Q, Cao XY, Cao H, Zhang ZG
Scientific reports631071(2016 Aug)

A Multiplex Kindred with Hennekam Syndrome due to Homozygosity for a CCBE1 Mutation that does not Prevent Protein Expression.
Jackson CC, Best L, Lorenzo L, Casanova JL, Wacker J, Bertz S, Agaimy A, Harrer T
Journal of clinical immunology36(1)19-27(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identify CCBE1 as a direct target of miR-330-3p, and show that knockdown of CCBE1 results in a greater invasive capacity. Accordingly, in breast cancer patients CCBE1 is frequently downregulated, and its loss is associated with reduced distant relapse-free and overall survival.
Title: Targeting of CCBE1 by miR-330-3p in human breast cancer promotes metastasis.

characterization of Hennekam Syndrome phenotypes in two Turkish siblings with protein mutation
Title: A Multiplex Kindred with Hennekam Syndrome due to Homozygosity for a CCBE1 Mutation that does not Prevent Protein Expression.

Collagen domains of CCBE1 are crucial for the activation of VEGFC in vitro and in vivo. The EGF domains of CCBE1 are dispensable for regulation of VEGFC processing in vitro, however, they are necessary for full lymphangiogenic activity of CCBE1 in vivo.
Title: Functional Dissection of the CCBE1 Protein: A Crucial Requirement for the Collagen Repeat Domain.

CCBE1 enhances lymphangiogenesis via A disintegrin and metalloprotease with thrombospondin motifs-3-mediated vascular endothelial growth factor-C activation.
Title: CCBE1 enhances lymphangiogenesis via A disintegrin and metalloprotease with thrombospondin motifs-3-mediated vascular endothelial growth factor-C activation.

Both siblings harbored a homozygous mutation in CCBE1.
Title: CCBE1 mutation in two siblings, one manifesting lymphedema-cholestasis syndrome, and the other, fetal hydrops.

The following CCBE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCBE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22240	NM_133459.4 Latest version!	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22240	XM_005266648.2 Latest version!	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22240	XM_024451091.1 Latest version!	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu87775	XM_017025556.1 Latest version!	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu87775	XM_017025557.1 Latest version!	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu87776	XM_017025558.1 Latest version!	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu22240	NM_133459.3	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCBE1 ( NM_133459.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_597716.1 Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), mRNA.

Price & Availability↑

CloneID	OHu22240
Clone ID Related Accession (Same CDS sequence)	NM_133459.4 , XM_024451091.1 , NM_133459.3 , XM_005266648.2
Accession Version	NM_133459.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	collagen and calcium-binding EGF domain-containing protein 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010776.10, AB075863.1 and AC016229.10. This sequence is a reference standard in the RefSeqGene project. On May 10, 2018 this sequence version replaced NM_133459.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB075863.1, AY358347.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000439986.9/ ENSP00000404464.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_597716.1
CDS	82..1302
Translation	MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDREICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQCTDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGPPGQPGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRDGSKGERGAPGPRGSPGPPGSFDFLLLMLADIRNDITELQEKVFGHRTHSSAEEFPLPQEFPSYPEAMDLGSGDDHPRRTETRDLRAPRDFYP

Target ORF information:

RefSeq Version	NM_133459.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133459.4

ORF Insert Sequence:

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCBE1 ( NM_133459.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_597716.1 Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), mRNA.

Price & Availability↑

CloneID	OHu22240
Clone ID Related Accession (Same CDS sequence)	NM_133459.4 , XM_024451091.1 , NM_133459.3 , XM_005266648.2
Accession Version	NM_133459.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	collagen and calcium-binding EGF domain-containing protein 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF434122.1, AY358347.1, CX782134.1, AC016229.10, EY892390.1 and BF515087.1. This sequence is a reference standard in the RefSeqGene project. On Mar 5, 2010 this sequence version replaced NM_133459.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB075863.1, AY358347.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_597716.1
CDS	71..1291
Translation	MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDREICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQCTDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGPPGQPGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRDGSKGERGAPGPRGSPGPPGSFDFLLLMLADIRNDITELQEKVFGHRTHSSAEEFPLPQEFPSYPEAMDLGSGDDHPRRTETRDLRAPRDFYP

Target ORF information:

RefSeq Version	NM_133459.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133459.3

ORF Insert Sequence:

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCBE1 ( XM_024451091.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306859.1 Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu22240
Clone ID Related Accession (Same CDS sequence)	NM_133459.4 , XM_024451091.1 , NM_133459.3 , XM_005266648.2
Accession Version	XM_024451091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306859.1
CDS	729..1949
Translation	MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDREICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQCTDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGPPGQPGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRDGSKGERGAPGPRGSPGPPGSFDFLLLMLADIRNDITELQEKVFGHRTHSSAEEFPLPQEFPSYPEAMDLGSGDDHPRRTETRDLRAPRDFYP

Target ORF information:

RefSeq Version	XM_024451091.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451091.1

ORF Insert Sequence:

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCBE1 ( XM_005266648.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005266705.1 Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22240
Clone ID Related Accession (Same CDS sequence)	NM_133459.4 , XM_024451091.1 , NM_133459.3 , XM_005266648.2
Accession Version	XM_005266648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
Comment

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005266705.1

Translation

MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDREICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQCTDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGPPGQPGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRDGSKGERGAPGPRGSPGPPGSFDFLLLMLADIRNDITELQEKVFGHRTHSSAEEFPLPQEFPSYPEAMDLGSGDDHPRRTETRDLRAPRDFYP

Target ORF information:

RefSeq Version	XM_005266648.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005266648.2

ORF Insert Sequence:

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGGA CCCCCTGGTT CTTTCGACTT CCTGCTACTT 
ATGCTGGCTG ACATCCGCAA TGACATCACT GAGCTGCAGG AAAAGGTGTT CGGGCACCGG 
ACTCACTCTT CAGCAGAGGA GTTCCCTTTA CCTCAGGAAT TTCCCAGCTA CCCAGAAGCC 
ATGGACCTGG GCTCTGGAGA TGACCATCCA AGAAGAACTG AGACAAGAGA CTTGAGAGCC 
CCCAGAGACT TCTACCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCBE1 ( XM_017025557.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881046.1 Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu87775
Clone ID Related Accession (Same CDS sequence)	XM_017025557.1 , XM_017025556.1
Accession Version	XM_017025557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGTGAG 
TGTGGAAGAG GGCACAGGAG GGTGATCACC AACAGAAAAA GCCTGCCAAA AGCTCACATT 
TGCTGGGGCC TGGACAATGT TCTCCAGCTT CCATCGAGGA GAAACAAGGC TCACCAGGAC 
CAAAGGGAAG CCCAGGCTTC CCCGGTATGC CAGGCCCTCC TGGGCAGCCC GGCCCACGGG 
GCTCAATGGG ACCCATGGGA CCATCTCCTG ATCTGTCCCA CATTAAGCAA GGCCGGAGGG 
GCCCTGTGGG TCCACCAGGG GCACCAGGAA GAGATGGTTC TAAGGGGGAG AGAGGAGCGC 
CTGGGCCCAG AGGGTCTCCA GGACCCCCTG GTTCTTTCGA CTTCCTGCTA CTTATGCTGG 
CTGACATCCG CAATGACATC ACTGAGCTGC AGGAAAAGGT GTTCGGGCAC CGGACTCACT 
CTTCAGCAGA GGAGTTCCCT TTACCTCAGG AATTTCCCAG CTACCCAGAA GCCATGGACC 
TGGGCTCTGG AGATGACCAT CCAAGAAGAA CTGAGACAAG AGACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881046.1
CDS	731..2038
Translation	MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDREICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQCTDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGECGRGHRRVITNRKSLPKAHICWGLDNVLQLPSRRNKAHQDQREAQASPVCQALLGSPAHGAQWDPWDHLLICPTLSKAGGALWVHQGHQEEMVLRGREERLGPEGLQDPLVLSTSCYLCWLTSAMTSLSCRKRCSGTGLTLQQRSSLYLRNFPATQKPWTWALEMTIQEELRQET

Target ORF information:

RefSeq Version	XM_017025557.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025557.1

ORF Insert Sequence:

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGTGAG 
TGTGGAAGAG GGCACAGGAG GGTGATCACC AACAGAAAAA GCCTGCCAAA AGCTCACATT 
TGCTGGGGCC TGGACAATGT TCTCCAGCTT CCATCGAGGA GAAACAAGGC TCACCAGGAC 
CAAAGGGAAG CCCAGGCTTC CCCGGTATGC CAGGCCCTCC TGGGCAGCCC GGCCCACGGG 
GCTCAATGGG ACCCATGGGA CCATCTCCTG ATCTGTCCCA CATTAAGCAA GGCCGGAGGG 
GCCCTGTGGG TCCACCAGGG GCACCAGGAA GAGATGGTTC TAAGGGGGAG AGAGGAGCGC 
CTGGGCCCAG AGGGTCTCCA GGACCCCCTG GTTCTTTCGA CTTCCTGCTA CTTATGCTGG 
CTGACATCCG CAATGACATC ACTGAGCTGC AGGAAAAGGT GTTCGGGCAC CGGACTCACT 
CTTCAGCAGA GGAGTTCCCT TTACCTCAGG AATTTCCCAG CTACCCAGAA GCCATGGACC 
TGGGCTCTGG AGATGACCAT CCAAGAAGAA CTGAGACAAG AGACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCBE1 ( XM_017025556.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881045.1 Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu87775
Clone ID Related Accession (Same CDS sequence)	XM_017025557.1 , XM_017025556.1
Accession Version	XM_017025556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGTGAG 
TGTGGAAGAG GGCACAGGAG GGTGATCACC AACAGAAAAA GCCTGCCAAA AGCTCACATT 
TGCTGGGGCC TGGACAATGT TCTCCAGCTT CCATCGAGGA GAAACAAGGC TCACCAGGAC 
CAAAGGGAAG CCCAGGCTTC CCCGGTATGC CAGGCCCTCC TGGGCAGCCC GGCCCACGGG 
GCTCAATGGG ACCCATGGGA CCATCTCCTG ATCTGTCCCA CATTAAGCAA GGCCGGAGGG 
GCCCTGTGGG TCCACCAGGG GCACCAGGAA GAGATGGTTC TAAGGGGGAG AGAGGAGCGC 
CTGGGCCCAG AGGGTCTCCA GGACCCCCTG GTTCTTTCGA CTTCCTGCTA CTTATGCTGG 
CTGACATCCG CAATGACATC ACTGAGCTGC AGGAAAAGGT GTTCGGGCAC CGGACTCACT 
CTTCAGCAGA GGAGTTCCCT TTACCTCAGG AATTTCCCAG CTACCCAGAA GCCATGGACC 
TGGGCTCTGG AGATGACCAT CCAAGAAGAA CTGAGACAAG AGACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881045.1
CDS	838..2145
Translation	MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDREICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQCTDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGECGRGHRRVITNRKSLPKAHICWGLDNVLQLPSRRNKAHQDQREAQASPVCQALLGSPAHGAQWDPWDHLLICPTLSKAGGALWVHQGHQEEMVLRGREERLGPEGLQDPLVLSTSCYLCWLTSAMTSLSCRKRCSGTGLTLQQRSSLYLRNFPATQKPWTWALEMTIQEELRQET

Target ORF information:

RefSeq Version	XM_017025556.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025556.1

ORF Insert Sequence:

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGTGAG 
TGTGGAAGAG GGCACAGGAG GGTGATCACC AACAGAAAAA GCCTGCCAAA AGCTCACATT 
TGCTGGGGCC TGGACAATGT TCTCCAGCTT CCATCGAGGA GAAACAAGGC TCACCAGGAC 
CAAAGGGAAG CCCAGGCTTC CCCGGTATGC CAGGCCCTCC TGGGCAGCCC GGCCCACGGG 
GCTCAATGGG ACCCATGGGA CCATCTCCTG ATCTGTCCCA CATTAAGCAA GGCCGGAGGG 
GCCCTGTGGG TCCACCAGGG GCACCAGGAA GAGATGGTTC TAAGGGGGAG AGAGGAGCGC 
CTGGGCCCAG AGGGTCTCCA GGACCCCCTG GTTCTTTCGA CTTCCTGCTA CTTATGCTGG 
CTGACATCCG CAATGACATC ACTGAGCTGC AGGAAAAGGT GTTCGGGCAC CGGACTCACT 
CTTCAGCAGA GGAGTTCCCT TTACCTCAGG AATTTCCCAG CTACCCAGAA GCCATGGACC 
TGGGCTCTGG AGATGACCAT CCAAGAAGAA CTGAGACAAG AGACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCBE1 ( XM_017025558.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881047.1 Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu87776
Clone ID Related Accession (Same CDS sequence)	XM_017025558.1
Accession Version	XM_017025558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen and calcium-binding EGF domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGTA AGTAGCACTC TGTGTCCTGC TTCCCCAGGG 
GAACGTTCTC AGGGATGCAG CTCTGATGAG CCTATAGGGA CCCCCTGGTT CTTTCGACTT 
CCTGCTACTT ATGCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881047.1
CDS	839..1939
Translation	MVPPPPSRGGAARGQLGRSLGPLLLLLALGHTWTYREEPEDGDREICSESKIATTKYPCLKSSGELTTCYRKKCCKGYKFVLGQCIPEDYDVCAEAPCEQQCTDNFGRVLCTCYPGYRYDRERHRKREKPYCLDIDECASSNGTLCAHICINTLGSYRCECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPGLPGGQGPPGSPGPKGSPGFPGMPGPPGQPGPRGSMGPMGPSPDLSHIKQGRRGPVGPPGAPGRDGSKGERGAPGPRGSPVSSTLCPASPGERSQGCSSDEPIGTPWFFRLPATYAG

Target ORF information:

RefSeq Version	XM_017025558.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen and calcium binding EGF domains 1 (CCBE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025558.1

ORF Insert Sequence:

ATGGTGCCGC CGCCTCCGAG CCGGGGAGGA GCTGCCAGGG GCCAGCTGGG CAGGAGCCTG 
GGTCCGCTGC TGCTGCTCCT GGCGTTGGGA CACACGTGGA CCTACAGAGA GGAGCCGGAG 
GACGGCGACA GAGAAATCTG CTCAGAGAGC AAAATCGCGA CGACTAAATA CCCGTGTCTG 
AAGTCTTCAG GCGAGCTCAC CACATGCTAC AGGAAAAAGT GCTGCAAAGG ATATAAATTT 
GTTCTTGGAC AATGCATCCC AGAAGATTAC GACGTTTGTG CCGAGGCTCC CTGTGAACAG 
CAGTGCACGG ACAACTTTGG CCGAGTGCTG TGTACTTGTT ATCCGGGATA CCGATATGAC 
CGGGAGAGAC ACCGGAAGCG GGAGAAGCCA TACTGTCTGG ATATTGATGA GTGTGCCAGC 
AGCAATGGGA CGCTGTGTGC CCACATCTGC ATCAATACCT TGGGCAGCTA CCGCTGCGAG 
TGCCGGGAAG GCTACATCCG GGAAGATGAT GGGAAGACAT GTACCAGGGG AGACAAATAT 
CCCAATGACA CTGGCCATGA GAAGTCTGAG AACATGGTGA AAGCCGGAAC TTGCTGTGCC 
ACATGCAAGG AGTTCTACCA GATGAAGCAG ACCGTGCTGC AGCTGAAGCA AAAGATTGCT 
CTGCTCCCCA ACAATGCAGC TGACCTGGGC AAGTATATCA CTGGTGACAA GGTGCTGGCC 
TCAAACACCT ACCTTCCAGG ACCTCCTGGC CTGCCTGGGG GCCAGGGCCC TCCCGGCTCA 
CCAGGACCAA AGGGAAGCCC AGGCTTCCCC GGTATGCCAG GCCCTCCTGG GCAGCCCGGC 
CCACGGGGCT CAATGGGACC CATGGGACCA TCTCCTGATC TGTCCCACAT TAAGCAAGGC 
CGGAGGGGCC CTGTGGGTCC ACCAGGGGCA CCAGGAAGAG ATGGTTCTAA GGGGGAGAGA 
GGAGCGCCTG GGCCCAGAGG GTCTCCAGTA AGTAGCACTC TGTGTCCTGC TTCCCCAGGG 
GAACGTTCTC AGGGATGCAG CTCTGATGAG CCTATAGGGA CCCCCTGGTT CTTTCGACTT 
CCTGCTACTT ATGCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical significance of CCBE1 expression in lung cancer.
Molecular medicine reports17(2)2107-2112(2018 Feb)
Li P,Cong Z,Qiang Y,Xiong L,Tang L,Zhang Y,Wu H,Yi J,Jing H,Li D,Shen Y

Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1.
Scientific reports7(1)4916(2017 Jul)
Jha SK,Rauniyar K,Karpanen T,Lepp?nen VM,Brouillard P,Vikkula M,Alitalo K,Jeltsch M

Targeting of CCBE1 by miR-330-3p in human breast cancer promotes metastasis.
British journal of cancer116(10)1350-1357(2017 May)
Mesci A,Huang X,Taeb S,Jahangiri S,Kim Y,Fokas E,Bruce J,Leong HS,Liu SK

CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib.
Scientific reports631071(2016 Aug)
Tian GA,Zhu CC,Zhang XX,Zhu L,Yang XM,Jiang SH,Li RK,Tu L,Wang Y,Zhuang C,He P,Li Q,Cao XY,Cao H,Zhang ZG

A Multiplex Kindred with Hennekam Syndrome due to Homozygosity for a CCBE1 Mutation that does not Prevent Protein Expression.
Journal of clinical immunology36(1)19-27(2016 Jan)
Jackson CC,Best L,Lorenzo L,Casanova JL,Wacker J,Bertz S,Agaimy A,Harrer T

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CCBE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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