fam214a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fam214a
Entrez Gene ID	555595
Full Name	family with sequence similarity 214, member A
Synonyms	si:dkey-266j9.3
General protein information	Preferred Names family with sequence similarity 214, member A Names protein FAM214A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173655.3	XP_005173712.1	protein FAM214A isoform X2
XM_017351926.1	XP_017207415.1	protein FAM214A isoform X3
XM_009293780.3	XP_009292055.1	protein FAM214A isoform X1
XM_009293780.2	XP_009292055.1	protein FAM214A isoform X1
NM_001202420.1	NP_001189349.1	protein FAM214A
XM_021467657.1	XP_021323332.1	protein FAM214A isoform X2
XM_005173655.4	XP_005173712.1	protein FAM214A isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	fam214a	NP_001189349.1
Xenopus tropicalis (tropical clawed frog)	fam214a	NP_001015702.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fam214a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fam214a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa55801	XM_017351926.1 Latest version!	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa42193	XM_021467657.1 Latest version!	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa38970	NM_001202420.1 Latest version!	Danio rerio family with sequence similarity 214 member A (fam214a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa23491	XM_005173655.4 Latest version!	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42192	XM_009293780.3 Latest version!	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa42192	XM_009293780.2	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa23491	XM_005173655.3	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fam214a ( XM_017351926.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207415.1 Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa55801
Clone ID Related Accession (Same CDS sequence)	XM_017351926.1
Accession Version	XM_017351926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM214A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGCCG CTGAGGAGTT CTTTGAATAT GATGCTGAGG AGTTTCTGGT GTTCCTGACA 
CTTTTGATCA CAGAAGGACG GACTCCAGAA TGCTCAGTAA AGGGGCGGAC GGAGGGCGTT 
CACTGCCCCC CGGCTCAGTC AGCTATGCCG GTCCTCACCA AACATGAATG CAGTGACAAA 
ATACCACAGT GTCGTCAGGC AAGGAGAACA AGATCAGAAG TCATGCTTCT GTGGCGCAAT 
CACATCCCTA TAATGATCGA GGTGATGCTG CTTCCTGATT GTTGCTATAG TGATGAAGGC 
CCAACCACAG ACTGCACAGA CCTAAATGAC CCAGCAATAA AGCAAGATGC ACTCTTACTA 
GAAAGATGGA CTTTACAGCC AGTTCCCAGA CAGAGTGGAG ATCGATTCAT AGAAGAGAAG 
ACCTTGCTGT TGGCGGTGCG CTCCTATGTG TTTTTCTCCC AGCTCAGTGC GTGGCTCAGT 
GCCTCTCACG GAATTGTCCC AAGAAACATC CTTTACAGAA TAAGTGCAGC TGATGAAGAA 
CTGATATGGA ACTTCTCACA AACACCCTCT GAACATGCTT TCCCTGTTCC AAATGTGTCA 
CATAGTGTCG CGCTGAAAGT GCGGGTTCAG TCTTTGCCTC GCCAGCCTAG ATACCCTGTA 
CTCAAATGTA GCATCCACTC AGGTCTAGCT TTTTTGGGTA AGAGAGCACT AGAGCATGGC 
GAGGGTGGCA ACCAAGCAGG AGACAACCGC AGTTCCCTTC GTCTGCCAAG ATCTCCACTT 
TTCTCCAGGT CTTTACACCC TTCTCCACCT TCCCACAGCC CTCTTAACAC CCGTAAGTGT 
CCCCCTCGTC CCGAGTCCCC TCTTCCACCA GGCAAAGCTG TCAAATGGCT GTACTCTCGG 
CTAAATGGCG GTATAGACAC CCCGCCTTCA GAACCATATA GCTTGTGTAC TAATGGGGCA 
GAGAGCCCAA AGACTTCAAG AACCGAATCA CCAATTCGTG GTTTTAAATC ACTCTCAATC 
ACAGACCCAT TAGTGATCCC AAGCCCCAGC TCCATCTCAG GTGAAACCAA CCCCCTTATT 
GGCTCCTTGC TTCAGGAAAG ACAGGAAGTC ATTGCTCGAA TTGCTCAGCG ACTTAATTTC 
TGCGACCCTA CAGCTCCCCA TCTCCCTGAT GCTCTCTTCA CTTCTCAAGA ACCCCCAGGA 
CACAAAACCA CCTGGAACTC GACACAGGAT AAGGAGTGTT TAAAGAAATC CAAAGATTCC 
CTCTTCTCAG TCCCTCATCC TCAAAATCAC AACGGAAACA GTCTAGAAAT CCCTGAGAGG 
AGCAGGTCTT CTCTCTTCGA CACCCCCTTG AGCCCTAGAA CCAGAACTCG GCTGGATAGA 
GTTGACCGTG AATCAAAAAC TTCCCCAAAA CCCGCAACAT GTAGGCGCTT GGTACTTAGT 
GACCAGTCTG CAGAGGGTTC TCTAATTGCA GATGCAGTTC AGGACATCTC CAGGTTGATT 
CAGGAGCGAC TGCAACATTC TTACAGTCTC CTGAATGGCA CCTATAAATT AAAGACTTCT 
CAGAATGAGC AGGTTGGCTC AAATCATGGA GCCCAAACAA ATGGCTTTGT ATCACTGTCA 
AGCCATAAGA AGCCTACAAA TGCACCCAAT GGTGAGGAAA GCACAGATCC TCATATTTCT 
CATGCAACAA AATGTTGCAG ATCTCCTGAC TCTAGTCGTA GAAAGCCAGA CTGTTCCCCT 
AGACCACTAA AAGTGGCCAG TTTAAAACTT GAAGACCACA GCGTCACAAA ATCACAGCCT 
TTAACTGCAA GTAATCACCA ACACTATGTC AGTAGAGAGT CCTGGACCTC CTTGAAAAAC 
AACTCTAGCC ATGCAAGTTC TCCTCAGGAG AATGGACTGA CCCAAATTGG ATACCATCAG 
CCCTTCAAGA ACCGAGTGGC AATCAGTGAA AAAGAAGCAG AAAAACACGT CCGGGACGGG 
TCCACTTGTC TTGAGAAAGA TGAGAACCAA GAACCCCATT CATCCCTTTC TAGCACACCA 
GCCAACCTTA CTTGCAACAT CTCCAGTCTT GCCCCCACAG AGAGCAATCA AACACAGAGA 
TGCTCCCCCA CCCCTTTGAA GTCATGCGGT AACTGGAAGA AACAGACTCG TCACTCCATA 
GATGGCACCG CAACAAAAGC CTTCCACCCC TGCACAGGCC TACCTCTCCT CTCCAGTCCT 
GTTCCTCAAA GAAAAAGCCA GACAGGATAT TTTGATTTAG ACACGTCTCT GATTCATTGC 
AGAGGTGTAC CATGGGCTGC CAATAGGAGG GTTTTGAAAA GATCACAAGA CTATGATGAG 
TCTCAGCATC AGATTCTCAG TGCCAGCGCT CCACCCGCCA ACCTCAGCCT TTTGGGTAAC 
TTTGAGGAGT GTGTTCTGAA TTACCGTCTG GAGCCGCTGG GTACTGTTGA GGGTTTTACA 
GCAGAAGTGG GCGCCAGCGG GACCTTCTGT CCTAGTCACA TGACCTTACC TGTGGACGTG 
TCATTCTACA GTGTCTCTGA CGATAACGCA CCTTCCCCAT ACATGGGTGT CATAAACTTG 
GAGTCACTCG GTAAAAGGGG ATATCGAGTA CCTCCTTCAG GAACCATTCA AGTGACCTTA 
TTTAACCCCA ATAAGACGGT GGTGAAGATG TTTGTGGTGA TGTACGATCT GAGAGATATG 
CCTGCCAGCC ATCAGACATT CCTGCGGCAG AGAACCTTTT CAGTCCCAGT CAAACGGGAG 
TTCAACGGAC AGAGCAACAA AAAGACGTCA CTGGGGCAAG GACGAACCCT CCGCTACCTC 
GTCCATCTGA GGTTCCAGAG TTCCAAATCT GGAAAGATCT ACCTCCATCG GGACATCCGG 
CTCCTTTTTT CCCGTAAATC CATGGAAGTG GACAGTGGTG CTGCCTATGA GCTCAAGTCA 
TTCACAGAGT TACCTGCCGA CCCTCCTTTC TCTCCCAGAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207415.1
CDS	79..3063
Translation	MYAAEEFFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSAMPVLTKHECSDKIPQCRQARRTRSEVMLLWRNHIPIMIEVMLLPDCCYSDEGPTTDCTDLNDPAIKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEELIWNFSQTPSEHAFPVPNVSHSVALKVRVQSLPRQPRYPVLKCSIHSGLAFLGKRALEHGEGGNQAGDNRSSLRLPRSPLFSRSLHPSPPSHSPLNTRKCPPRPESPLPPGKAVKWLYSRLNGGIDTPPSEPYSLCTNGAESPKTSRTESPIRGFKSLSITDPLVIPSPSSISGETNPLIGSLLQERQEVIARIAQRLNFCDPTAPHLPDALFTSQEPPGHKTTWNSTQDKECLKKSKDSLFSVPHPQNHNGNSLEIPERSRSSLFDTPLSPRTRTRLDRVDRESKTSPKPATCRRLVLSDQSAEGSLIADAVQDISRLIQERLQHSYSLLNGTYKLKTSQNEQVGSNHGAQTNGFVSLSSHKKPTNAPNGEESTDPHISHATKCCRSPDSSRRKPDCSPRPLKVASLKLEDHSVTKSQPLTASNHQHYVSRESWTSLKNNSSHASSPQENGLTQIGYHQPFKNRVAISEKEAEKHVRDGSTCLEKDENQEPHSSLSSTPANLTCNISSLAPTESNQTQRCSPTPLKSCGNWKKQTRHSIDGTATKAFHPCTGLPLLSSPVPQRKSQTGYFDLDTSLIHCRGVPWAANRRVLKRSQDYDESQHQILSASAPPANLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGTFCPSHMTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVMYDLRDMPASHQTFLRQRTFSVPVKREFNGQSNKKTSLGQGRTLRYLVHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSFTELPADPPFSPRC

Target ORF information:

RefSeq Version	XM_017351926.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351926.1

ORF Insert Sequence:

ATGTATGCCG CTGAGGAGTT CTTTGAATAT GATGCTGAGG AGTTTCTGGT GTTCCTGACA 
CTTTTGATCA CAGAAGGACG GACTCCAGAA TGCTCAGTAA AGGGGCGGAC GGAGGGCGTT 
CACTGCCCCC CGGCTCAGTC AGCTATGCCG GTCCTCACCA AACATGAATG CAGTGACAAA 
ATACCACAGT GTCGTCAGGC AAGGAGAACA AGATCAGAAG TCATGCTTCT GTGGCGCAAT 
CACATCCCTA TAATGATCGA GGTGATGCTG CTTCCTGATT GTTGCTATAG TGATGAAGGC 
CCAACCACAG ACTGCACAGA CCTAAATGAC CCAGCAATAA AGCAAGATGC ACTCTTACTA 
GAAAGATGGA CTTTACAGCC AGTTCCCAGA CAGAGTGGAG ATCGATTCAT AGAAGAGAAG 
ACCTTGCTGT TGGCGGTGCG CTCCTATGTG TTTTTCTCCC AGCTCAGTGC GTGGCTCAGT 
GCCTCTCACG GAATTGTCCC AAGAAACATC CTTTACAGAA TAAGTGCAGC TGATGAAGAA 
CTGATATGGA ACTTCTCACA AACACCCTCT GAACATGCTT TCCCTGTTCC AAATGTGTCA 
CATAGTGTCG CGCTGAAAGT GCGGGTTCAG TCTTTGCCTC GCCAGCCTAG ATACCCTGTA 
CTCAAATGTA GCATCCACTC AGGTCTAGCT TTTTTGGGTA AGAGAGCACT AGAGCATGGC 
GAGGGTGGCA ACCAAGCAGG AGACAACCGC AGTTCCCTTC GTCTGCCAAG ATCTCCACTT 
TTCTCCAGGT CTTTACACCC TTCTCCACCT TCCCACAGCC CTCTTAACAC CCGTAAGTGT 
CCCCCTCGTC CCGAGTCCCC TCTTCCACCA GGCAAAGCTG TCAAATGGCT GTACTCTCGG 
CTAAATGGCG GTATAGACAC CCCGCCTTCA GAACCATATA GCTTGTGTAC TAATGGGGCA 
GAGAGCCCAA AGACTTCAAG AACCGAATCA CCAATTCGTG GTTTTAAATC ACTCTCAATC 
ACAGACCCAT TAGTGATCCC AAGCCCCAGC TCCATCTCAG GTGAAACCAA CCCCCTTATT 
GGCTCCTTGC TTCAGGAAAG ACAGGAAGTC ATTGCTCGAA TTGCTCAGCG ACTTAATTTC 
TGCGACCCTA CAGCTCCCCA TCTCCCTGAT GCTCTCTTCA CTTCTCAAGA ACCCCCAGGA 
CACAAAACCA CCTGGAACTC GACACAGGAT AAGGAGTGTT TAAAGAAATC CAAAGATTCC 
CTCTTCTCAG TCCCTCATCC TCAAAATCAC AACGGAAACA GTCTAGAAAT CCCTGAGAGG 
AGCAGGTCTT CTCTCTTCGA CACCCCCTTG AGCCCTAGAA CCAGAACTCG GCTGGATAGA 
GTTGACCGTG AATCAAAAAC TTCCCCAAAA CCCGCAACAT GTAGGCGCTT GGTACTTAGT 
GACCAGTCTG CAGAGGGTTC TCTAATTGCA GATGCAGTTC AGGACATCTC CAGGTTGATT 
CAGGAGCGAC TGCAACATTC TTACAGTCTC CTGAATGGCA CCTATAAATT AAAGACTTCT 
CAGAATGAGC AGGTTGGCTC AAATCATGGA GCCCAAACAA ATGGCTTTGT ATCACTGTCA 
AGCCATAAGA AGCCTACAAA TGCACCCAAT GGTGAGGAAA GCACAGATCC TCATATTTCT 
CATGCAACAA AATGTTGCAG ATCTCCTGAC TCTAGTCGTA GAAAGCCAGA CTGTTCCCCT 
AGACCACTAA AAGTGGCCAG TTTAAAACTT GAAGACCACA GCGTCACAAA ATCACAGCCT 
TTAACTGCAA GTAATCACCA ACACTATGTC AGTAGAGAGT CCTGGACCTC CTTGAAAAAC 
AACTCTAGCC ATGCAAGTTC TCCTCAGGAG AATGGACTGA CCCAAATTGG ATACCATCAG 
CCCTTCAAGA ACCGAGTGGC AATCAGTGAA AAAGAAGCAG AAAAACACGT CCGGGACGGG 
TCCACTTGTC TTGAGAAAGA TGAGAACCAA GAACCCCATT CATCCCTTTC TAGCACACCA 
GCCAACCTTA CTTGCAACAT CTCCAGTCTT GCCCCCACAG AGAGCAATCA AACACAGAGA 
TGCTCCCCCA CCCCTTTGAA GTCATGCGGT AACTGGAAGA AACAGACTCG TCACTCCATA 
GATGGCACCG CAACAAAAGC CTTCCACCCC TGCACAGGCC TACCTCTCCT CTCCAGTCCT 
GTTCCTCAAA GAAAAAGCCA GACAGGATAT TTTGATTTAG ACACGTCTCT GATTCATTGC 
AGAGGTGTAC CATGGGCTGC CAATAGGAGG GTTTTGAAAA GATCACAAGA CTATGATGAG 
TCTCAGCATC AGATTCTCAG TGCCAGCGCT CCACCCGCCA ACCTCAGCCT TTTGGGTAAC 
TTTGAGGAGT GTGTTCTGAA TTACCGTCTG GAGCCGCTGG GTACTGTTGA GGGTTTTACA 
GCAGAAGTGG GCGCCAGCGG GACCTTCTGT CCTAGTCACA TGACCTTACC TGTGGACGTG 
TCATTCTACA GTGTCTCTGA CGATAACGCA CCTTCCCCAT ACATGGGTGT CATAAACTTG 
GAGTCACTCG GTAAAAGGGG ATATCGAGTA CCTCCTTCAG GAACCATTCA AGTGACCTTA 
TTTAACCCCA ATAAGACGGT GGTGAAGATG TTTGTGGTGA TGTACGATCT GAGAGATATG 
CCTGCCAGCC ATCAGACATT CCTGCGGCAG AGAACCTTTT CAGTCCCAGT CAAACGGGAG 
TTCAACGGAC AGAGCAACAA AAAGACGTCA CTGGGGCAAG GACGAACCCT CCGCTACCTC 
GTCCATCTGA GGTTCCAGAG TTCCAAATCT GGAAAGATCT ACCTCCATCG GGACATCCGG 
CTCCTTTTTT CCCGTAAATC CATGGAAGTG GACAGTGGTG CTGCCTATGA GCTCAAGTCA 
TTCACAGAGT TACCTGCCGA CCCTCCTTTC TCTCCCAGAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam214a ( XM_021467657.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323332.1 Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42193
Clone ID Related Accession (Same CDS sequence)	XM_021467657.1
Accession Version	XM_021467657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM214A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATTGTCAC 
CATAGAAGAG CGCTTCATCA ACCAGATGCC GCTGAGGAGT TCTTTGAATA TGATGCTGAG 
GAGTTTCTGG TGTTCCTGAC ACTTTTGATC ACAGAAGGAC GGACTCCAGA ATGCTCAGTA 
AAGGGGCGGA CGGAGGGCGT TCACTGCCCC CCGGCTCAGT CAGCTATGCC GGTCCTCACC 
AAACATGAAT GCAGTGACAA AATACCACAG TGTCGTCAGG CAAGGAGAAC AAGATCAGAA 
GTCATGCTTC TGTGGCGCAA TCACATCCCT ATAATGATCG AGGTGATGCT GCTTCCTGAT 
TGTTGCTATA GTGATGAAGG CCCAACCACA GACTGCACAG ACCTAAATGA CCCAGCAATA 
AAGCAAGATG CACTCTTACT AGAAAGATGG ACTTTACAGC CAGTTCCCAG ACAGAGTGGA 
GATCGATTCA TAGAAGAGAA GACCTTGCTG TTGGCGGTGC GCTCCTATGT GTTTTTCTCC 
CAGCTCAGTG CGTGGCTCAG TGCCTCTCAC GGAATTGTCC CAAGAAACAT CCTTTACAGA 
ATAAGTGCAG CTGATGAAGA ACTGATATGG AACTTCTCAC AAACACCCTC TGAACATGCT 
TTCCCTGTTC CAAATGTGTC ACATAGTGTC GCGCTGAAAG TGCGGGTTCA GTCTTTGCCT 
CGCCAGCCTA GATACCCTGT ACTCAAATGT AGCATCCACT CAGGTCTAGC TTTTTTGGGT 
AAGAGAGCAC TAGAGCATGG CGAGGGTGGC AACCAAGCAG GAGACAACCG CAGTTCCCTT 
CGTCTGCCAA GATCTCCACT TTTCTCCAGG TCTTTACACC CTTCTCCACC TTCCCACAGC 
CCTCTTAACA CCCGTAAGTG TCCCCCTCGT CCCGAGTCCC CTCTTCCACC AGGCAAAGCT 
GTCAAATGGC TGTACTCTCG GCTAAATGGC GGTATAGACA CCCCGCCTTC AGAACCATAT 
AGCTTGTGTA CTAATGGGGC AGAGAGCCCA AAGACTTCAA GAACCGAATC ACCAATTCGT 
GGTTTTAAAT CACTCTCAAT CACAGACCCA TTAGTGATCC CAAGCCCCAG CTCCATCTCA 
GGTGAAACCA ACCCCCTTAT TGGCTCCTTG CTTCAGGAAA GACAGGAAGT CATTGCTCGA 
ATTGCTCAGC GACTTAATTT CTGCGACCCT ACAGCTCCCC ATCTCCCTGA TGCTCTCTTC 
ACTTCTCAAG AACCCCCAGG ACACAAAACC ACCTGGAACT CGACACAGGA TAAGGAGTGT 
TTAAAGAAAT CCAAAGATTC CCTCTTCTCA GTCCCTCATC CTCAAAATCA CAACGGAAAC 
AGTCTAGAAA TCCCTGAGAG GAGCAGGTCT TCTCTCTTCG ACACCCCCTT GAGCCCTAGA 
ACCAGAACTC GGCTGGATAG AGTTGACCGT GAATCAAAAA CTTCCCCAAA ACCCGCAACA 
TGTAGGCGCT TGGTACTTAG TGACCAGTCT GCAGAGGGTT CTCTAATTGC AGATGCAGTT 
CAGGACATCT CCAGGTTGAT TCAGGAGCGA CTGCAACATT CTTACAGTCT CCTGAATGGC 
ACCTATAAAT TAAAGACTTC TCAGAATGAG CAGGTTGGCT CAAATCATGG AGCCCAAACA 
AATGGCTTTG TATCACTGTC AAGCCATAAG AAGCCTACAA ATGCACCCAA TGGTGAGGAA 
AGCACAGATC CTCATATTTC TCATGCAACA AAATGTTGCA GATCTCCTGA CTCTAGTCGT 
AGAAAGCCAG ACTGTTCCCC TAGACCACTA AAAGTGGCCA GTTTAAAACT TGAAGACCAC 
AGCGTCACAA AATCACAGCC TTTAACTGCA AGTAATCACC AACACTATGT CAGTAGAGAG 
TCCTGGACCT CCTTGAAAAA CAACTCTAGC CATGCAAGTT CTCCTCAGGA GAATGGACTG 
ACCCAAATTG GATACCATCA GCCCTTCAAG AACCGAGTGG CAATCAGTGA AAAAGAAGCA 
GAAAAACACG TCCGGGACGG GTCCACTTGT CTTGAGAAAG ATGAGAACCA AGAACCCCAT 
TCATCCCTTT CTAGCACACC AGCCAACCTT ACTTGCAACA TCTCCAGTCT TGCCCCCACA 
GAGAGCAATC AAACAAGATG CTCCCCCACC CCTTTGAAGT CATGCGGTAA CTGGAAGAAA 
CAGACTCGTC ACTCCATAGA TGGCACCGCA ACAAAAGCCT TCCACCCCTG CACAGGCCTA 
CCTCTCCTCT CCAGTCCTGT TCCTCAAAGA AAAAGCCAGA CAGGATATTT TGATTTAGAC 
ACGTCTCTGA TTCATTGCAG AGGTGTACCA TGGGCTGCCA ATAGGAGGGT TTTGAAAAGA 
TCACAAGACT ATGATGAGTC TCAGCATCAG ATTCTCAGTG CCAGCGCTCC ACCCGCCAAC 
CTCAGCCTTT TGGGTAACTT TGAGGAGTGT GTTCTGAATT ACCGTCTGGA GCCGCTGGGT 
ACTGTTGAGG GTTTTACAGC AGAAGTGGGC GCCAGCGGGA CCTTCTGTCC TAGTCACATG 
ACCTTACCTG TGGACGTGTC ATTCTACAGT GTCTCTGACG ATAACGCACC TTCCCCATAC 
ATGGGTGTCA TAAACTTGGA GTCACTCGGT AAAAGGGGAT ATCGAGTACC TCCTTCAGGA 
ACCATTCAAG TGACCTTATT TAACCCCAAT AAGACGGTGG TGAAGATGTT TGTGGTGATG 
TACGATCTGA GAGATATGCC TGCCAGCCAT CAGACATTCC TGCGGCAGAG AACCTTTTCA 
GTCCCAGTCA AACGGGAGTT CAACGGACAG AGCAACAAAA AGACGTCACT GGGGCAAGGA 
CGAACCCTCC GCTACCTCGT CCATCTGAGG TTCCAGAGTT CCAAATCTGG AAAGATCTAC 
CTCCATCGGG ACATCCGGCT CCTTTTTTCC CGTAAATCCA TGGAAGTGGA CAGTGGTGCT 
GCCTATGAGC TCAAGTCATT CACAGAGTTA CCTGCCGACC CTCCTTTCTC TCCCAGATGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323332.1
CDS	734..3856
Translation	MSKGRVFKSKQRSEDGRRTAGILEGITVSVTNMKPDRDCHHRRALHQPDAAEEFFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSAMPVLTKHECSDKIPQCRQARRTRSEVMLLWRNHIPIMIEVMLLPDCCYSDEGPTTDCTDLNDPAIKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEELIWNFSQTPSEHAFPVPNVSHSVALKVRVQSLPRQPRYPVLKCSIHSGLAFLGKRALEHGEGGNQAGDNRSSLRLPRSPLFSRSLHPSPPSHSPLNTRKCPPRPESPLPPGKAVKWLYSRLNGGIDTPPSEPYSLCTNGAESPKTSRTESPIRGFKSLSITDPLVIPSPSSISGETNPLIGSLLQERQEVIARIAQRLNFCDPTAPHLPDALFTSQEPPGHKTTWNSTQDKECLKKSKDSLFSVPHPQNHNGNSLEIPERSRSSLFDTPLSPRTRTRLDRVDRESKTSPKPATCRRLVLSDQSAEGSLIADAVQDISRLIQERLQHSYSLLNGTYKLKTSQNEQVGSNHGAQTNGFVSLSSHKKPTNAPNGEESTDPHISHATKCCRSPDSSRRKPDCSPRPLKVASLKLEDHSVTKSQPLTASNHQHYVSRESWTSLKNNSSHASSPQENGLTQIGYHQPFKNRVAISEKEAEKHVRDGSTCLEKDENQEPHSSLSSTPANLTCNISSLAPTESNQTRCSPTPLKSCGNWKKQTRHSIDGTATKAFHPCTGLPLLSSPVPQRKSQTGYFDLDTSLIHCRGVPWAANRRVLKRSQDYDESQHQILSASAPPANLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGTFCPSHMTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVMYDLRDMPASHQTFLRQRTFSVPVKREFNGQSNKKTSLGQGRTLRYLVHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSFTELPADPPFSPRC

Target ORF information:

RefSeq Version	XM_021467657.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467657.1

ORF Insert Sequence:

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATTGTCAC 
CATAGAAGAG CGCTTCATCA ACCAGATGCC GCTGAGGAGT TCTTTGAATA TGATGCTGAG 
GAGTTTCTGG TGTTCCTGAC ACTTTTGATC ACAGAAGGAC GGACTCCAGA ATGCTCAGTA 
AAGGGGCGGA CGGAGGGCGT TCACTGCCCC CCGGCTCAGT CAGCTATGCC GGTCCTCACC 
AAACATGAAT GCAGTGACAA AATACCACAG TGTCGTCAGG CAAGGAGAAC AAGATCAGAA 
GTCATGCTTC TGTGGCGCAA TCACATCCCT ATAATGATCG AGGTGATGCT GCTTCCTGAT 
TGTTGCTATA GTGATGAAGG CCCAACCACA GACTGCACAG ACCTAAATGA CCCAGCAATA 
AAGCAAGATG CACTCTTACT AGAAAGATGG ACTTTACAGC CAGTTCCCAG ACAGAGTGGA 
GATCGATTCA TAGAAGAGAA GACCTTGCTG TTGGCGGTGC GCTCCTATGT GTTTTTCTCC 
CAGCTCAGTG CGTGGCTCAG TGCCTCTCAC GGAATTGTCC CAAGAAACAT CCTTTACAGA 
ATAAGTGCAG CTGATGAAGA ACTGATATGG AACTTCTCAC AAACACCCTC TGAACATGCT 
TTCCCTGTTC CAAATGTGTC ACATAGTGTC GCGCTGAAAG TGCGGGTTCA GTCTTTGCCT 
CGCCAGCCTA GATACCCTGT ACTCAAATGT AGCATCCACT CAGGTCTAGC TTTTTTGGGT 
AAGAGAGCAC TAGAGCATGG CGAGGGTGGC AACCAAGCAG GAGACAACCG CAGTTCCCTT 
CGTCTGCCAA GATCTCCACT TTTCTCCAGG TCTTTACACC CTTCTCCACC TTCCCACAGC 
CCTCTTAACA CCCGTAAGTG TCCCCCTCGT CCCGAGTCCC CTCTTCCACC AGGCAAAGCT 
GTCAAATGGC TGTACTCTCG GCTAAATGGC GGTATAGACA CCCCGCCTTC AGAACCATAT 
AGCTTGTGTA CTAATGGGGC AGAGAGCCCA AAGACTTCAA GAACCGAATC ACCAATTCGT 
GGTTTTAAAT CACTCTCAAT CACAGACCCA TTAGTGATCC CAAGCCCCAG CTCCATCTCA 
GGTGAAACCA ACCCCCTTAT TGGCTCCTTG CTTCAGGAAA GACAGGAAGT CATTGCTCGA 
ATTGCTCAGC GACTTAATTT CTGCGACCCT ACAGCTCCCC ATCTCCCTGA TGCTCTCTTC 
ACTTCTCAAG AACCCCCAGG ACACAAAACC ACCTGGAACT CGACACAGGA TAAGGAGTGT 
TTAAAGAAAT CCAAAGATTC CCTCTTCTCA GTCCCTCATC CTCAAAATCA CAACGGAAAC 
AGTCTAGAAA TCCCTGAGAG GAGCAGGTCT TCTCTCTTCG ACACCCCCTT GAGCCCTAGA 
ACCAGAACTC GGCTGGATAG AGTTGACCGT GAATCAAAAA CTTCCCCAAA ACCCGCAACA 
TGTAGGCGCT TGGTACTTAG TGACCAGTCT GCAGAGGGTT CTCTAATTGC AGATGCAGTT 
CAGGACATCT CCAGGTTGAT TCAGGAGCGA CTGCAACATT CTTACAGTCT CCTGAATGGC 
ACCTATAAAT TAAAGACTTC TCAGAATGAG CAGGTTGGCT CAAATCATGG AGCCCAAACA 
AATGGCTTTG TATCACTGTC AAGCCATAAG AAGCCTACAA ATGCACCCAA TGGTGAGGAA 
AGCACAGATC CTCATATTTC TCATGCAACA AAATGTTGCA GATCTCCTGA CTCTAGTCGT 
AGAAAGCCAG ACTGTTCCCC TAGACCACTA AAAGTGGCCA GTTTAAAACT TGAAGACCAC 
AGCGTCACAA AATCACAGCC TTTAACTGCA AGTAATCACC AACACTATGT CAGTAGAGAG 
TCCTGGACCT CCTTGAAAAA CAACTCTAGC CATGCAAGTT CTCCTCAGGA GAATGGACTG 
ACCCAAATTG GATACCATCA GCCCTTCAAG AACCGAGTGG CAATCAGTGA AAAAGAAGCA 
GAAAAACACG TCCGGGACGG GTCCACTTGT CTTGAGAAAG ATGAGAACCA AGAACCCCAT 
TCATCCCTTT CTAGCACACC AGCCAACCTT ACTTGCAACA TCTCCAGTCT TGCCCCCACA 
GAGAGCAATC AAACAAGATG CTCCCCCACC CCTTTGAAGT CATGCGGTAA CTGGAAGAAA 
CAGACTCGTC ACTCCATAGA TGGCACCGCA ACAAAAGCCT TCCACCCCTG CACAGGCCTA 
CCTCTCCTCT CCAGTCCTGT TCCTCAAAGA AAAAGCCAGA CAGGATATTT TGATTTAGAC 
ACGTCTCTGA TTCATTGCAG AGGTGTACCA TGGGCTGCCA ATAGGAGGGT TTTGAAAAGA 
TCACAAGACT ATGATGAGTC TCAGCATCAG ATTCTCAGTG CCAGCGCTCC ACCCGCCAAC 
CTCAGCCTTT TGGGTAACTT TGAGGAGTGT GTTCTGAATT ACCGTCTGGA GCCGCTGGGT 
ACTGTTGAGG GTTTTACAGC AGAAGTGGGC GCCAGCGGGA CCTTCTGTCC TAGTCACATG 
ACCTTACCTG TGGACGTGTC ATTCTACAGT GTCTCTGACG ATAACGCACC TTCCCCATAC 
ATGGGTGTCA TAAACTTGGA GTCACTCGGT AAAAGGGGAT ATCGAGTACC TCCTTCAGGA 
ACCATTCAAG TGACCTTATT TAACCCCAAT AAGACGGTGG TGAAGATGTT TGTGGTGATG 
TACGATCTGA GAGATATGCC TGCCAGCCAT CAGACATTCC TGCGGCAGAG AACCTTTTCA 
GTCCCAGTCA AACGGGAGTT CAACGGACAG AGCAACAAAA AGACGTCACT GGGGCAAGGA 
CGAACCCTCC GCTACCTCGT CCATCTGAGG TTCCAGAGTT CCAAATCTGG AAAGATCTAC 
CTCCATCGGG ACATCCGGCT CCTTTTTTCC CGTAAATCCA TGGAAGTGGA CAGTGGTGCT 
GCCTATGAGC TCAAGTCATT CACAGAGTTA CCTGCCGACC CTCCTTTCTC TCCCAGATGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam214a ( NM_001202420.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001189349.1 Danio rerio family with sequence similarity 214 member A (fam214a), mRNA.

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CloneID	ODa38970
Clone ID Related Accession (Same CDS sequence)	NM_001202420.1
Accession Version	NM_001202420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	protein FAM214A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX908748.7 and BX936456.6. On Jan 29, 2011 this sequence version replaced XM_678146.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATGCCGCT 
GAGGAGTTCT TTGAATATGA TGCTGAGGAG TTTCTGGTGT TCCTGACACT TTTGATCACA 
GAAGGACGGA CTCCAGAATG CTCAGTAAAG GGGCGGACGG AGGGCGTTCA CTGCCCCCCG 
GCTCAGTCAG CTATGCCGGT CCTCACCAAA CATGAATGCA GTGACAAAAT ACCACAGTGT 
CGTCAGGCAA GGAGAACAAG ATCAGAAGTC ATGCTTCTGT GGCGCAATCA CATCCCTATA 
ATGATCGAGG TGATGCTGCT TCCTGATTGT TGCTATAGTG ATGAAGGCCC AACCACAGAC 
TGCACAGACC TAAATGACCC AGCAATAAAG CAAGATGCAC TCTTACTAGA AAGATGGACT 
TTACAGCCAG TTCCCAGACA GAGTGGAGAT CGATTCATAG AAGAGAAGAC CTTGCTGTTG 
GCGGTGCGCT CCTATGTGTT TTTCTCCCAG CTCAGTGCGT GGCTCAGTGC CTCTCACGGA 
ATTGTCCCAA GAAACATCCT TTACAGAATA AGTGCAGCTG ATGAAGAACT GATATGGAAC 
TTCTCACAAA CACCCTCTGA ACATGCTTTC CCTGTTCCAA ATGTGTCACA TAGTGTCGCG 
CTGAAAGTGC GGGTTCAGTC TTTGCCTCGC CAGCCTAGAT ACCCTGTACT CAAATGTAGC 
ATCCACTCAG GTCTAGCTTT TTTGGGTAAG AGAGCACTAG AGCATGGCGA GGGTGGCAAC 
CAAGCAGGAG ACAACCGCAG TTCCCTTCGT CTGCCAAGAT CTCCACTTTT CTCCAGGTCT 
TTACACCCTT CTCCACCTTC CCACAGCCCT CTTAACACCC GTAAGTGTCC CCCTCGTCCC 
GAGTCCCCTC TTCCACCAGG CAAAGCTGTC AAATGGCTGT ACTCTCGGCT AAATGGCGGT 
ATAGACACCC CGCCTTCAGA ACCATATAGC TTGTGTACTA ATGGGGCAGA GAGCCCAAAG 
ACTTCAAGAA CCGAATCACC AATTCGTGGT TTTAAATCAC TCTCAATCAC AGACCCATTA 
GTGATCCCAA GCCCCAGCTC CATCTCAGGT GAAACCAACC CCCTTATTGG CTCCTTGCTT 
CAGGAAAGAC AGGAAGTCAT TGCTCGAATT GCTCAGCGAC TTAATTTCTG CGACCCTACA 
GCTCCCCATC TCCCTGATGC TCTCTTCACT TCTCAAGAAC CCCCAGGACA CAAAACCACC 
TGGAACTCGA CACAGGATAA GGAGTGTTTA AAGAAATCCA AAGATTCCCT CTTCTCAGTC 
CCTCATCCTC AAAATCACAA CGGAAACAGT CTAGAAATCC CTGAGAGGAG CAGGTCTTCT 
CTCTTCGACA CCCCCTTGAG CCCTAGAACC AGAACTCGGC TGGATAGAGT TGACCGTGAA 
TCAAAAACTT CCCCAAAACC CGCAACATGT AGGCGCTTGG TACTTAGTGA CCAGTCTGCA 
GAGGGTTCTC TAATTGCAGA TGCAGTTCAG GACATCTCCA GGTTGATTCA GGAGCGACTG 
CAACATTCTT ACAGTCTCCT GAATGGCACC TATAAATTAA AGACTTCTCA GAATGAGCAG 
GTTGGCTCAA ATCATGGAGC CCAAACAAAT GGCTTTGTAT CACTGTCAAG CCATAAGAAG 
CCTACAAATG CACCCAATGG TGAGGAAAGC ACAGATCCTC ATATTTCTCA TGCAACAAAA 
TGTTGCAGAT CTCCTGACTC TAGTCGTAGA AAGCCAGACT GTTCCCCTAG ACCACTAAAA 
GTGGCCAGTT TAAAACTTGA AGACCACAGC GTCACAAAAT CACAGCCTTT AACTGCAAGT 
AATCACCAAC ACTATGTCAG TAGAGAGTCC TGGACCTCCT TGAAAAACAA CTCTAGCCAT 
GCAAGTTCTC CTCAGGAGAA TGGACTGACC CAAATTGGAT ACCATCAGCC CTTCAAGAAC 
CGAGTGGCAA TCAGTGAAAA AGAAGCAGAA AAACACGTCC GGGACGGGTC CACTTGTCTT 
GAGAAAGATG AGAACCAAGA ACCCCATTCA TCCCTTTCTA GCACACCAGC CAACCTTACT 
TGCAACATCT CCAGTCTTGC CCCCACAGAG AGCAATCAAA CATCATGCGG TAACTGGAAG 
AAACAGACTC GTCACTCCAT AGATGGCACC GCAACAAAAG CCTTCCACCC CTGCACAGGC 
CTACCTCTCC TCTCCAGTCC TGTTCCTCAA AGAAAAAGCC AGACAGGATA TTTTGATTTA 
GACACGTCTC TGATTCATTG CAGAGGTGTA CCATGGGCTG CCAATAGGAG GGTTTTGAAA 
AGATCACAAG ACTATGATGA GTCTCAGCAT CAGATTCTCA GTGCCAGCGC TCCACCCGCC 
AACCTCAGCC TTTTGGGTAA CTTTGAGGAG TGTGTTCTGA ATTACCGTCT GGAGCCGCTG 
GGTACTGTTG AGGGTTTTAC AGCAGAAGTG GGCGCCAGCG GGACCTTCTG TCCTAGTCAC 
ATGACCTTAC CTGTGGACGT GTCATTCTAC AGTGTCTCTG ACGATAACGC ACCTTCCCCA 
TACATGGGTG TCATAAACTT GGAGTCACTC GGTAAAAGGG GATATCGAGT ACCTCCTTCA 
GGAACCATTC AAGTGACCTT ATTTAACCCC AATAAGACGG TGGTGAAGAT GTTTGTGGTG 
ATGTACGATC TGAGAGATAT GCCTGCCAGC CATCAGACAT TCCTGCGGCA GAGAACCTTT 
TCAGTCCCAG TCAAACGGGA GTTCAACGGA CAGAGCAACA AAAAGACGTC ACTGGGGCAA 
GGACGAACCC TCCGCTACCT CGTCCATCTG AGGTTCCAGA GTTCCAAATC TGGAAAGATC 
TACCTCCATC GGGACATCCG GCTCCTTTTT TCCCGTAAAT CCATGGAAGT GGACAGTGGT 
GCTGCCTATG AGCTCAAGTC ATTCACAGAG TTACCTGCCG ACCCTCCTTT CTCTCCCAGA 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189349.1
CDS	194..3259
Translation	MSKGRVFKSKQRSEDGRRTAGILEGITVSVTNMKPDRDAAEEFFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSAMPVLTKHECSDKIPQCRQARRTRSEVMLLWRNHIPIMIEVMLLPDCCYSDEGPTTDCTDLNDPAIKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEELIWNFSQTPSEHAFPVPNVSHSVALKVRVQSLPRQPRYPVLKCSIHSGLAFLGKRALEHGEGGNQAGDNRSSLRLPRSPLFSRSLHPSPPSHSPLNTRKCPPRPESPLPPGKAVKWLYSRLNGGIDTPPSEPYSLCTNGAESPKTSRTESPIRGFKSLSITDPLVIPSPSSISGETNPLIGSLLQERQEVIARIAQRLNFCDPTAPHLPDALFTSQEPPGHKTTWNSTQDKECLKKSKDSLFSVPHPQNHNGNSLEIPERSRSSLFDTPLSPRTRTRLDRVDRESKTSPKPATCRRLVLSDQSAEGSLIADAVQDISRLIQERLQHSYSLLNGTYKLKTSQNEQVGSNHGAQTNGFVSLSSHKKPTNAPNGEESTDPHISHATKCCRSPDSSRRKPDCSPRPLKVASLKLEDHSVTKSQPLTASNHQHYVSRESWTSLKNNSSHASSPQENGLTQIGYHQPFKNRVAISEKEAEKHVRDGSTCLEKDENQEPHSSLSSTPANLTCNISSLAPTESNQTSCGNWKKQTRHSIDGTATKAFHPCTGLPLLSSPVPQRKSQTGYFDLDTSLIHCRGVPWAANRRVLKRSQDYDESQHQILSASAPPANLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGTFCPSHMTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVMYDLRDMPASHQTFLRQRTFSVPVKREFNGQSNKKTSLGQGRTLRYLVHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSFTELPADPPFSPRC

Target ORF information:

RefSeq Version	NM_001202420.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 214 member A (fam214a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202420.1

ORF Insert Sequence:

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATGCCGCT 
GAGGAGTTCT TTGAATATGA TGCTGAGGAG TTTCTGGTGT TCCTGACACT TTTGATCACA 
GAAGGACGGA CTCCAGAATG CTCAGTAAAG GGGCGGACGG AGGGCGTTCA CTGCCCCCCG 
GCTCAGTCAG CTATGCCGGT CCTCACCAAA CATGAATGCA GTGACAAAAT ACCACAGTGT 
CGTCAGGCAA GGAGAACAAG ATCAGAAGTC ATGCTTCTGT GGCGCAATCA CATCCCTATA 
ATGATCGAGG TGATGCTGCT TCCTGATTGT TGCTATAGTG ATGAAGGCCC AACCACAGAC 
TGCACAGACC TAAATGACCC AGCAATAAAG CAAGATGCAC TCTTACTAGA AAGATGGACT 
TTACAGCCAG TTCCCAGACA GAGTGGAGAT CGATTCATAG AAGAGAAGAC CTTGCTGTTG 
GCGGTGCGCT CCTATGTGTT TTTCTCCCAG CTCAGTGCGT GGCTCAGTGC CTCTCACGGA 
ATTGTCCCAA GAAACATCCT TTACAGAATA AGTGCAGCTG ATGAAGAACT GATATGGAAC 
TTCTCACAAA CACCCTCTGA ACATGCTTTC CCTGTTCCAA ATGTGTCACA TAGTGTCGCG 
CTGAAAGTGC GGGTTCAGTC TTTGCCTCGC CAGCCTAGAT ACCCTGTACT CAAATGTAGC 
ATCCACTCAG GTCTAGCTTT TTTGGGTAAG AGAGCACTAG AGCATGGCGA GGGTGGCAAC 
CAAGCAGGAG ACAACCGCAG TTCCCTTCGT CTGCCAAGAT CTCCACTTTT CTCCAGGTCT 
TTACACCCTT CTCCACCTTC CCACAGCCCT CTTAACACCC GTAAGTGTCC CCCTCGTCCC 
GAGTCCCCTC TTCCACCAGG CAAAGCTGTC AAATGGCTGT ACTCTCGGCT AAATGGCGGT 
ATAGACACCC CGCCTTCAGA ACCATATAGC TTGTGTACTA ATGGGGCAGA GAGCCCAAAG 
ACTTCAAGAA CCGAATCACC AATTCGTGGT TTTAAATCAC TCTCAATCAC AGACCCATTA 
GTGATCCCAA GCCCCAGCTC CATCTCAGGT GAAACCAACC CCCTTATTGG CTCCTTGCTT 
CAGGAAAGAC AGGAAGTCAT TGCTCGAATT GCTCAGCGAC TTAATTTCTG CGACCCTACA 
GCTCCCCATC TCCCTGATGC TCTCTTCACT TCTCAAGAAC CCCCAGGACA CAAAACCACC 
TGGAACTCGA CACAGGATAA GGAGTGTTTA AAGAAATCCA AAGATTCCCT CTTCTCAGTC 
CCTCATCCTC AAAATCACAA CGGAAACAGT CTAGAAATCC CTGAGAGGAG CAGGTCTTCT 
CTCTTCGACA CCCCCTTGAG CCCTAGAACC AGAACTCGGC TGGATAGAGT TGACCGTGAA 
TCAAAAACTT CCCCAAAACC CGCAACATGT AGGCGCTTGG TACTTAGTGA CCAGTCTGCA 
GAGGGTTCTC TAATTGCAGA TGCAGTTCAG GACATCTCCA GGTTGATTCA GGAGCGACTG 
CAACATTCTT ACAGTCTCCT GAATGGCACC TATAAATTAA AGACTTCTCA GAATGAGCAG 
GTTGGCTCAA ATCATGGAGC CCAAACAAAT GGCTTTGTAT CACTGTCAAG CCATAAGAAG 
CCTACAAATG CACCCAATGG TGAGGAAAGC ACAGATCCTC ATATTTCTCA TGCAACAAAA 
TGTTGCAGAT CTCCTGACTC TAGTCGTAGA AAGCCAGACT GTTCCCCTAG ACCACTAAAA 
GTGGCCAGTT TAAAACTTGA AGACCACAGC GTCACAAAAT CACAGCCTTT AACTGCAAGT 
AATCACCAAC ACTATGTCAG TAGAGAGTCC TGGACCTCCT TGAAAAACAA CTCTAGCCAT 
GCAAGTTCTC CTCAGGAGAA TGGACTGACC CAAATTGGAT ACCATCAGCC CTTCAAGAAC 
CGAGTGGCAA TCAGTGAAAA AGAAGCAGAA AAACACGTCC GGGACGGGTC CACTTGTCTT 
GAGAAAGATG AGAACCAAGA ACCCCATTCA TCCCTTTCTA GCACACCAGC CAACCTTACT 
TGCAACATCT CCAGTCTTGC CCCCACAGAG AGCAATCAAA CATCATGCGG TAACTGGAAG 
AAACAGACTC GTCACTCCAT AGATGGCACC GCAACAAAAG CCTTCCACCC CTGCACAGGC 
CTACCTCTCC TCTCCAGTCC TGTTCCTCAA AGAAAAAGCC AGACAGGATA TTTTGATTTA 
GACACGTCTC TGATTCATTG CAGAGGTGTA CCATGGGCTG CCAATAGGAG GGTTTTGAAA 
AGATCACAAG ACTATGATGA GTCTCAGCAT CAGATTCTCA GTGCCAGCGC TCCACCCGCC 
AACCTCAGCC TTTTGGGTAA CTTTGAGGAG TGTGTTCTGA ATTACCGTCT GGAGCCGCTG 
GGTACTGTTG AGGGTTTTAC AGCAGAAGTG GGCGCCAGCG GGACCTTCTG TCCTAGTCAC 
ATGACCTTAC CTGTGGACGT GTCATTCTAC AGTGTCTCTG ACGATAACGC ACCTTCCCCA 
TACATGGGTG TCATAAACTT GGAGTCACTC GGTAAAAGGG GATATCGAGT ACCTCCTTCA 
GGAACCATTC AAGTGACCTT ATTTAACCCC AATAAGACGG TGGTGAAGAT GTTTGTGGTG 
ATGTACGATC TGAGAGATAT GCCTGCCAGC CATCAGACAT TCCTGCGGCA GAGAACCTTT 
TCAGTCCCAG TCAAACGGGA GTTCAACGGA CAGAGCAACA AAAAGACGTC ACTGGGGCAA 
GGACGAACCC TCCGCTACCT CGTCCATCTG AGGTTCCAGA GTTCCAAATC TGGAAAGATC 
TACCTCCATC GGGACATCCG GCTCCTTTTT TCCCGTAAAT CCATGGAAGT GGACAGTGGT 
GCTGCCTATG AGCTCAAGTC ATTCACAGAG TTACCTGCCG ACCCTCCTTT CTCTCCCAGA 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam214a ( XM_005173655.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173712.1 Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X2, mRNA.

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CloneID	ODa23491
Clone ID Related Accession (Same CDS sequence)	XM_005173655.3 , XM_005173655.4
Accession Version	XM_005173655.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM214A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATGCCGCT 
GAGGAGTTCT TTGAATATGA TGCTGAGGAG TTTCTGGTGT TCCTGACACT TTTGATCACA 
GAAGGACGGA CTCCAGAATG CTCAGTAAAG GGGCGGACGG AGGGCGTTCA CTGCCCCCCG 
GCTCAGTCAG CTATGCCGGT CCTCACCAAA CATGAATGCA GTGACAAAAT ACCACAGTGT 
CGTCAGGCAA GGAGAACAAG ATCAGAAGTC ATGCTTCTGT GGCGCAATCA CATCCCTATA 
ATGATCGAGG TGATGCTGCT TCCTGATTGT TGCTATAGTG ATGAAGGCCC AACCACAGAC 
TGCACAGACC TAAATGACCC AGCAATAAAG CAAGATGCAC TCTTACTAGA AAGATGGACT 
TTACAGCCAG TTCCCAGACA GAGTGGAGAT CGATTCATAG AAGAGAAGAC CTTGCTGTTG 
GCGGTGCGCT CCTATGTGTT TTTCTCCCAG CTCAGTGCGT GGCTCAGTGC CTCTCACGGA 
ATTGTCCCAA GAAACATCCT TTACAGAATA AGTGCAGCTG ATGAAGAACT GATATGGAAC 
TTCTCACAAA CACCCTCTGA ACATGCTTTC CCTGTTCCAA ATGTGTCACA TAGTGTCGCG 
CTGAAAGTGC GGGTTCAGTC TTTGCCTCGC CAGCCTAGAT ACCCTGTACT CAAATGTAGC 
ATCCACTCAG GTCTAGCTTT TTTGGGTAAG AGAGCACTAG AGCATGGCGA GGGTGGCAAC 
CAAGCAGGAG ACAACCGCAG TTCCCTTCGT CTGCCAAGAT CTCCACTTTT CTCCAGGTCT 
TTACACCCTT CTCCACCTTC CCACAGCCCT CTTAACACCC GTAAGTGTCC CCCTCGTCCC 
GAGTCCCCTC TTCCACCAGG CAAAGCTGTC AAATGGCTGT ACTCTCGGCT AAATGGCGGT 
ATAGACACCC CGCCTTCAGA ACCATATAGC TTGTGTACTA ATGGGGCAGA GAGCCCAAAG 
ACTTCAAGAA CCGAATCACC AATTCGTGGT TTTAAATCAC TCTCAATCAC AGACCCATTA 
GTGATCCCAA GCCCCAGCTC CATCTCAGGT GAAACCAACC CCCTTATTGG CTCCTTGCTT 
CAGGAAAGAC AGGAAGTCAT TGCTCGAATT GCTCAGCGAC TTAATTTCTG CGACCCTACA 
GCTCCCCATC TCCCTGATGC TCTCTTCACT TCTCAAGAAC CCCCAGGACA CAAAACCACC 
TGGAACTCGA CACAGGATAA GGAGTGTTTA AAGAAATCCA AAGATTCCCT CTTCTCAGTC 
CCTCATCCTC AAAATCACAA CGGAAACAGT CTAGAAATCC CTGAGAGGAG CAGGTCTTCT 
CTCTTCGACA CCCCCTTGAG CCCTAGAACC AGAACTCGGC TGGATAGAGT TGACCGTGAA 
TCAAAAACTT CCCCAAAACC CGCAACATGT AGGCGCTTGG TACTTAGTGA CCAGTCTGCA 
GAGGGTTCTC TAATTGCAGA TGCAGTTCAG GACATCTCCA GGTTGATTCA GGAGCGACTG 
CAACATTCTT ACAGTCTCCT GAATGGCACC TATAAATTAA AGACTTCTCA GAATGAGCAG 
GTTGGCTCAA ATCATGGAGC CCAAACAAAT GGCTTTGTAT CACTGTCAAG CCATAAGAAG 
CCTACAAATG CACCCAATGG TGAGGAAAGC ACAGATCCTC ATATTTCTCA TGCAACAAAA 
TGTTGCAGAT CTCCTGACTC TAGTCGTAGA AAGCCAGACT GTTCCCCTAG ACCACTAAAA 
GTGGCCAGTT TAAAACTTGA AGACCACAGC GTCACAAAAT CACAGCCTTT AACTGCAAGT 
AATCACCAAC ACTATGTCAG TAGAGAGTCC TGGACCTCCT TGAAAAACAA CTCTAGCCAT 
GCAAGTTCTC CTCAGGAGAA TGGACTGACC CAAATTGGAT ACCATCAGCC CTTCAAGAAC 
CGAGTGGCAA TCAGTGAAAA AGAAGCAGAA AAACACGTCC GGGACGGGTC CACTTGTCTT 
GAGAAAGATG AGAACCAAGA ACCCCATTCA TCCCTTTCTA GCACACCAGC CAACCTTACT 
TGCAACATCT CCAGTCTTGC CCCCACAGAG AGCAATCAAA CACAGAGATG CTCCCCCACC 
CCTTTGAAGT CATGCGGTAA CTGGAAGAAA CAGACTCGTC ACTCCATAGA TGGCACCGCA 
ACAAAAGCCT TCCACCCCTG CACAGGCCTA CCTCTCCTCT CCAGTCCTGT TCCTCAAAGA 
AAAAGCCAGA CAGGATATTT TGATTTAGAC ACGTCTCTGA TTCATTGCAG AGGTGTACCA 
TGGGCTGCCA ATAGGAGGGT TTTGAAAAGA TCACAAGACT ATGATGAGTC TCAGCATCAG 
ATTCTCAGTG CCAGCGCTCC ACCCGCCAAC CTCAGCCTTT TGGGTAACTT TGAGGAGTGT 
GTTCTGAATT ACCGTCTGGA GCCGCTGGGT ACTGTTGAGG GTTTTACAGC AGAAGTGGGC 
GCCAGCGGGA CCTTCTGTCC TAGTCACATG ACCTTACCTG TGGACGTGTC ATTCTACAGT 
GTCTCTGACG ATAACGCACC TTCCCCATAC ATGGGTGTCA TAAACTTGGA GTCACTCGGT 
AAAAGGGGAT ATCGAGTACC TCCTTCAGGA ACCATTCAAG TGACCTTATT TAACCCCAAT 
AAGACGGTGG TGAAGATGTT TGTGGTGATG TACGATCTGA GAGATATGCC TGCCAGCCAT 
CAGACATTCC TGCGGCAGAG AACCTTTTCA GTCCCAGTCA AACGGGAGTT CAACGGACAG 
AGCAACAAAA AGACGTCACT GGGGCAAGGA CGAACCCTCC GCTACCTCGT CCATCTGAGG 
TTCCAGAGTT CCAAATCTGG AAAGATCTAC CTCCATCGGG ACATCCGGCT CCTTTTTTCC 
CGTAAATCCA TGGAAGTGGA CAGTGGTGCT GCCTATGAGC TCAAGTCATT CACAGAGTTA 
CCTGCCGACC CTCCTTTCTC TCCCAGATGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173712.1
CDS	714..3806
Translation	MSKGRVFKSKQRSEDGRRTAGILEGITVSVTNMKPDRDAAEEFFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSAMPVLTKHECSDKIPQCRQARRTRSEVMLLWRNHIPIMIEVMLLPDCCYSDEGPTTDCTDLNDPAIKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEELIWNFSQTPSEHAFPVPNVSHSVALKVRVQSLPRQPRYPVLKCSIHSGLAFLGKRALEHGEGGNQAGDNRSSLRLPRSPLFSRSLHPSPPSHSPLNTRKCPPRPESPLPPGKAVKWLYSRLNGGIDTPPSEPYSLCTNGAESPKTSRTESPIRGFKSLSITDPLVIPSPSSISGETNPLIGSLLQERQEVIARIAQRLNFCDPTAPHLPDALFTSQEPPGHKTTWNSTQDKECLKKSKDSLFSVPHPQNHNGNSLEIPERSRSSLFDTPLSPRTRTRLDRVDRESKTSPKPATCRRLVLSDQSAEGSLIADAVQDISRLIQERLQHSYSLLNGTYKLKTSQNEQVGSNHGAQTNGFVSLSSHKKPTNAPNGEESTDPHISHATKCCRSPDSSRRKPDCSPRPLKVASLKLEDHSVTKSQPLTASNHQHYVSRESWTSLKNNSSHASSPQENGLTQIGYHQPFKNRVAISEKEAEKHVRDGSTCLEKDENQEPHSSLSSTPANLTCNISSLAPTESNQTQRCSPTPLKSCGNWKKQTRHSIDGTATKAFHPCTGLPLLSSPVPQRKSQTGYFDLDTSLIHCRGVPWAANRRVLKRSQDYDESQHQILSASAPPANLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGTFCPSHMTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVMYDLRDMPASHQTFLRQRTFSVPVKREFNGQSNKKTSLGQGRTLRYLVHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSFTELPADPPFSPRC

Target ORF information:

RefSeq Version	XM_005173655.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173655.3

ORF Insert Sequence:

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATGCCGCT 
GAGGAGTTCT TTGAATATGA TGCTGAGGAG TTTCTGGTGT TCCTGACACT TTTGATCACA 
GAAGGACGGA CTCCAGAATG CTCAGTAAAG GGGCGGACGG AGGGCGTTCA CTGCCCCCCG 
GCTCAGTCAG CTATGCCGGT CCTCACCAAA CATGAATGCA GTGACAAAAT ACCACAGTGT 
CGTCAGGCAA GGAGAACAAG ATCAGAAGTC ATGCTTCTGT GGCGCAATCA CATCCCTATA 
ATGATCGAGG TGATGCTGCT TCCTGATTGT TGCTATAGTG ATGAAGGCCC AACCACAGAC 
TGCACAGACC TAAATGACCC AGCAATAAAG CAAGATGCAC TCTTACTAGA AAGATGGACT 
TTACAGCCAG TTCCCAGACA GAGTGGAGAT CGATTCATAG AAGAGAAGAC CTTGCTGTTG 
GCGGTGCGCT CCTATGTGTT TTTCTCCCAG CTCAGTGCGT GGCTCAGTGC CTCTCACGGA 
ATTGTCCCAA GAAACATCCT TTACAGAATA AGTGCAGCTG ATGAAGAACT GATATGGAAC 
TTCTCACAAA CACCCTCTGA ACATGCTTTC CCTGTTCCAA ATGTGTCACA TAGTGTCGCG 
CTGAAAGTGC GGGTTCAGTC TTTGCCTCGC CAGCCTAGAT ACCCTGTACT CAAATGTAGC 
ATCCACTCAG GTCTAGCTTT TTTGGGTAAG AGAGCACTAG AGCATGGCGA GGGTGGCAAC 
CAAGCAGGAG ACAACCGCAG TTCCCTTCGT CTGCCAAGAT CTCCACTTTT CTCCAGGTCT 
TTACACCCTT CTCCACCTTC CCACAGCCCT CTTAACACCC GTAAGTGTCC CCCTCGTCCC 
GAGTCCCCTC TTCCACCAGG CAAAGCTGTC AAATGGCTGT ACTCTCGGCT AAATGGCGGT 
ATAGACACCC CGCCTTCAGA ACCATATAGC TTGTGTACTA ATGGGGCAGA GAGCCCAAAG 
ACTTCAAGAA CCGAATCACC AATTCGTGGT TTTAAATCAC TCTCAATCAC AGACCCATTA 
GTGATCCCAA GCCCCAGCTC CATCTCAGGT GAAACCAACC CCCTTATTGG CTCCTTGCTT 
CAGGAAAGAC AGGAAGTCAT TGCTCGAATT GCTCAGCGAC TTAATTTCTG CGACCCTACA 
GCTCCCCATC TCCCTGATGC TCTCTTCACT TCTCAAGAAC CCCCAGGACA CAAAACCACC 
TGGAACTCGA CACAGGATAA GGAGTGTTTA AAGAAATCCA AAGATTCCCT CTTCTCAGTC 
CCTCATCCTC AAAATCACAA CGGAAACAGT CTAGAAATCC CTGAGAGGAG CAGGTCTTCT 
CTCTTCGACA CCCCCTTGAG CCCTAGAACC AGAACTCGGC TGGATAGAGT TGACCGTGAA 
TCAAAAACTT CCCCAAAACC CGCAACATGT AGGCGCTTGG TACTTAGTGA CCAGTCTGCA 
GAGGGTTCTC TAATTGCAGA TGCAGTTCAG GACATCTCCA GGTTGATTCA GGAGCGACTG 
CAACATTCTT ACAGTCTCCT GAATGGCACC TATAAATTAA AGACTTCTCA GAATGAGCAG 
GTTGGCTCAA ATCATGGAGC CCAAACAAAT GGCTTTGTAT CACTGTCAAG CCATAAGAAG 
CCTACAAATG CACCCAATGG TGAGGAAAGC ACAGATCCTC ATATTTCTCA TGCAACAAAA 
TGTTGCAGAT CTCCTGACTC TAGTCGTAGA AAGCCAGACT GTTCCCCTAG ACCACTAAAA 
GTGGCCAGTT TAAAACTTGA AGACCACAGC GTCACAAAAT CACAGCCTTT AACTGCAAGT 
AATCACCAAC ACTATGTCAG TAGAGAGTCC TGGACCTCCT TGAAAAACAA CTCTAGCCAT 
GCAAGTTCTC CTCAGGAGAA TGGACTGACC CAAATTGGAT ACCATCAGCC CTTCAAGAAC 
CGAGTGGCAA TCAGTGAAAA AGAAGCAGAA AAACACGTCC GGGACGGGTC CACTTGTCTT 
GAGAAAGATG AGAACCAAGA ACCCCATTCA TCCCTTTCTA GCACACCAGC CAACCTTACT 
TGCAACATCT CCAGTCTTGC CCCCACAGAG AGCAATCAAA CACAGAGATG CTCCCCCACC 
CCTTTGAAGT CATGCGGTAA CTGGAAGAAA CAGACTCGTC ACTCCATAGA TGGCACCGCA 
ACAAAAGCCT TCCACCCCTG CACAGGCCTA CCTCTCCTCT CCAGTCCTGT TCCTCAAAGA 
AAAAGCCAGA CAGGATATTT TGATTTAGAC ACGTCTCTGA TTCATTGCAG AGGTGTACCA 
TGGGCTGCCA ATAGGAGGGT TTTGAAAAGA TCACAAGACT ATGATGAGTC TCAGCATCAG 
ATTCTCAGTG CCAGCGCTCC ACCCGCCAAC CTCAGCCTTT TGGGTAACTT TGAGGAGTGT 
GTTCTGAATT ACCGTCTGGA GCCGCTGGGT ACTGTTGAGG GTTTTACAGC AGAAGTGGGC 
GCCAGCGGGA CCTTCTGTCC TAGTCACATG ACCTTACCTG TGGACGTGTC ATTCTACAGT 
GTCTCTGACG ATAACGCACC TTCCCCATAC ATGGGTGTCA TAAACTTGGA GTCACTCGGT 
AAAAGGGGAT ATCGAGTACC TCCTTCAGGA ACCATTCAAG TGACCTTATT TAACCCCAAT 
AAGACGGTGG TGAAGATGTT TGTGGTGATG TACGATCTGA GAGATATGCC TGCCAGCCAT 
CAGACATTCC TGCGGCAGAG AACCTTTTCA GTCCCAGTCA AACGGGAGTT CAACGGACAG 
AGCAACAAAA AGACGTCACT GGGGCAAGGA CGAACCCTCC GCTACCTCGT CCATCTGAGG 
TTCCAGAGTT CCAAATCTGG AAAGATCTAC CTCCATCGGG ACATCCGGCT CCTTTTTTCC 
CGTAAATCCA TGGAAGTGGA CAGTGGTGCT GCCTATGAGC TCAAGTCATT CACAGAGTTA 
CCTGCCGACC CTCCTTTCTC TCCCAGATGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam214a ( XM_005173655.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173712.1 Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa23491
Clone ID Related Accession (Same CDS sequence)	XM_005173655.3 , XM_005173655.4
Accession Version	XM_005173655.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM214A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173655.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATGCCGCT 
GAGGAGTTCT TTGAATATGA TGCTGAGGAG TTTCTGGTGT TCCTGACACT TTTGATCACA 
GAAGGACGGA CTCCAGAATG CTCAGTAAAG GGGCGGACGG AGGGCGTTCA CTGCCCCCCG 
GCTCAGTCAG CTATGCCGGT CCTCACCAAA CATGAATGCA GTGACAAAAT ACCACAGTGT 
CGTCAGGCAA GGAGAACAAG ATCAGAAGTC ATGCTTCTGT GGCGCAATCA CATCCCTATA 
ATGATCGAGG TGATGCTGCT TCCTGATTGT TGCTATAGTG ATGAAGGCCC AACCACAGAC 
TGCACAGACC TAAATGACCC AGCAATAAAG CAAGATGCAC TCTTACTAGA AAGATGGACT 
TTACAGCCAG TTCCCAGACA GAGTGGAGAT CGATTCATAG AAGAGAAGAC CTTGCTGTTG 
GCGGTGCGCT CCTATGTGTT TTTCTCCCAG CTCAGTGCGT GGCTCAGTGC CTCTCACGGA 
ATTGTCCCAA GAAACATCCT TTACAGAATA AGTGCAGCTG ATGAAGAACT GATATGGAAC 
TTCTCACAAA CACCCTCTGA ACATGCTTTC CCTGTTCCAA ATGTGTCACA TAGTGTCGCG 
CTGAAAGTGC GGGTTCAGTC TTTGCCTCGC CAGCCTAGAT ACCCTGTACT CAAATGTAGC 
ATCCACTCAG GTCTAGCTTT TTTGGGTAAG AGAGCACTAG AGCATGGCGA GGGTGGCAAC 
CAAGCAGGAG ACAACCGCAG TTCCCTTCGT CTGCCAAGAT CTCCACTTTT CTCCAGGTCT 
TTACACCCTT CTCCACCTTC CCACAGCCCT CTTAACACCC GTAAGTGTCC CCCTCGTCCC 
GAGTCCCCTC TTCCACCAGG CAAAGCTGTC AAATGGCTGT ACTCTCGGCT AAATGGCGGT 
ATAGACACCC CGCCTTCAGA ACCATATAGC TTGTGTACTA ATGGGGCAGA GAGCCCAAAG 
ACTTCAAGAA CCGAATCACC AATTCGTGGT TTTAAATCAC TCTCAATCAC AGACCCATTA 
GTGATCCCAA GCCCCAGCTC CATCTCAGGT GAAACCAACC CCCTTATTGG CTCCTTGCTT 
CAGGAAAGAC AGGAAGTCAT TGCTCGAATT GCTCAGCGAC TTAATTTCTG CGACCCTACA 
GCTCCCCATC TCCCTGATGC TCTCTTCACT TCTCAAGAAC CCCCAGGACA CAAAACCACC 
TGGAACTCGA CACAGGATAA GGAGTGTTTA AAGAAATCCA AAGATTCCCT CTTCTCAGTC 
CCTCATCCTC AAAATCACAA CGGAAACAGT CTAGAAATCC CTGAGAGGAG CAGGTCTTCT 
CTCTTCGACA CCCCCTTGAG CCCTAGAACC AGAACTCGGC TGGATAGAGT TGACCGTGAA 
TCAAAAACTT CCCCAAAACC CGCAACATGT AGGCGCTTGG TACTTAGTGA CCAGTCTGCA 
GAGGGTTCTC TAATTGCAGA TGCAGTTCAG GACATCTCCA GGTTGATTCA GGAGCGACTG 
CAACATTCTT ACAGTCTCCT GAATGGCACC TATAAATTAA AGACTTCTCA GAATGAGCAG 
GTTGGCTCAA ATCATGGAGC CCAAACAAAT GGCTTTGTAT CACTGTCAAG CCATAAGAAG 
CCTACAAATG CACCCAATGG TGAGGAAAGC ACAGATCCTC ATATTTCTCA TGCAACAAAA 
TGTTGCAGAT CTCCTGACTC TAGTCGTAGA AAGCCAGACT GTTCCCCTAG ACCACTAAAA 
GTGGCCAGTT TAAAACTTGA AGACCACAGC GTCACAAAAT CACAGCCTTT AACTGCAAGT 
AATCACCAAC ACTATGTCAG TAGAGAGTCC TGGACCTCCT TGAAAAACAA CTCTAGCCAT 
GCAAGTTCTC CTCAGGAGAA TGGACTGACC CAAATTGGAT ACCATCAGCC CTTCAAGAAC 
CGAGTGGCAA TCAGTGAAAA AGAAGCAGAA AAACACGTCC GGGACGGGTC CACTTGTCTT 
GAGAAAGATG AGAACCAAGA ACCCCATTCA TCCCTTTCTA GCACACCAGC CAACCTTACT 
TGCAACATCT CCAGTCTTGC CCCCACAGAG AGCAATCAAA CACAGAGATG CTCCCCCACC 
CCTTTGAAGT CATGCGGTAA CTGGAAGAAA CAGACTCGTC ACTCCATAGA TGGCACCGCA 
ACAAAAGCCT TCCACCCCTG CACAGGCCTA CCTCTCCTCT CCAGTCCTGT TCCTCAAAGA 
AAAAGCCAGA CAGGATATTT TGATTTAGAC ACGTCTCTGA TTCATTGCAG AGGTGTACCA 
TGGGCTGCCA ATAGGAGGGT TTTGAAAAGA TCACAAGACT ATGATGAGTC TCAGCATCAG 
ATTCTCAGTG CCAGCGCTCC ACCCGCCAAC CTCAGCCTTT TGGGTAACTT TGAGGAGTGT 
GTTCTGAATT ACCGTCTGGA GCCGCTGGGT ACTGTTGAGG GTTTTACAGC AGAAGTGGGC 
GCCAGCGGGA CCTTCTGTCC TAGTCACATG ACCTTACCTG TGGACGTGTC ATTCTACAGT 
GTCTCTGACG ATAACGCACC TTCCCCATAC ATGGGTGTCA TAAACTTGGA GTCACTCGGT 
AAAAGGGGAT ATCGAGTACC TCCTTCAGGA ACCATTCAAG TGACCTTATT TAACCCCAAT 
AAGACGGTGG TGAAGATGTT TGTGGTGATG TACGATCTGA GAGATATGCC TGCCAGCCAT 
CAGACATTCC TGCGGCAGAG AACCTTTTCA GTCCCAGTCA AACGGGAGTT CAACGGACAG 
AGCAACAAAA AGACGTCACT GGGGCAAGGA CGAACCCTCC GCTACCTCGT CCATCTGAGG 
TTCCAGAGTT CCAAATCTGG AAAGATCTAC CTCCATCGGG ACATCCGGCT CCTTTTTTCC 
CGTAAATCCA TGGAAGTGGA CAGTGGTGCT GCCTATGAGC TCAAGTCATT CACAGAGTTA 
CCTGCCGACC CTCCTTTCTC TCCCAGATGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173712.1
CDS	734..3826
Translation	MSKGRVFKSKQRSEDGRRTAGILEGITVSVTNMKPDRDAAEEFFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSAMPVLTKHECSDKIPQCRQARRTRSEVMLLWRNHIPIMIEVMLLPDCCYSDEGPTTDCTDLNDPAIKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEELIWNFSQTPSEHAFPVPNVSHSVALKVRVQSLPRQPRYPVLKCSIHSGLAFLGKRALEHGEGGNQAGDNRSSLRLPRSPLFSRSLHPSPPSHSPLNTRKCPPRPESPLPPGKAVKWLYSRLNGGIDTPPSEPYSLCTNGAESPKTSRTESPIRGFKSLSITDPLVIPSPSSISGETNPLIGSLLQERQEVIARIAQRLNFCDPTAPHLPDALFTSQEPPGHKTTWNSTQDKECLKKSKDSLFSVPHPQNHNGNSLEIPERSRSSLFDTPLSPRTRTRLDRVDRESKTSPKPATCRRLVLSDQSAEGSLIADAVQDISRLIQERLQHSYSLLNGTYKLKTSQNEQVGSNHGAQTNGFVSLSSHKKPTNAPNGEESTDPHISHATKCCRSPDSSRRKPDCSPRPLKVASLKLEDHSVTKSQPLTASNHQHYVSRESWTSLKNNSSHASSPQENGLTQIGYHQPFKNRVAISEKEAEKHVRDGSTCLEKDENQEPHSSLSSTPANLTCNISSLAPTESNQTQRCSPTPLKSCGNWKKQTRHSIDGTATKAFHPCTGLPLLSSPVPQRKSQTGYFDLDTSLIHCRGVPWAANRRVLKRSQDYDESQHQILSASAPPANLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGTFCPSHMTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVMYDLRDMPASHQTFLRQRTFSVPVKREFNGQSNKKTSLGQGRTLRYLVHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSFTELPADPPFSPRC

Target ORF information:

RefSeq Version	XM_005173655.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173655.4

ORF Insert Sequence:

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATGCCGCT 
GAGGAGTTCT TTGAATATGA TGCTGAGGAG TTTCTGGTGT TCCTGACACT TTTGATCACA 
GAAGGACGGA CTCCAGAATG CTCAGTAAAG GGGCGGACGG AGGGCGTTCA CTGCCCCCCG 
GCTCAGTCAG CTATGCCGGT CCTCACCAAA CATGAATGCA GTGACAAAAT ACCACAGTGT 
CGTCAGGCAA GGAGAACAAG ATCAGAAGTC ATGCTTCTGT GGCGCAATCA CATCCCTATA 
ATGATCGAGG TGATGCTGCT TCCTGATTGT TGCTATAGTG ATGAAGGCCC AACCACAGAC 
TGCACAGACC TAAATGACCC AGCAATAAAG CAAGATGCAC TCTTACTAGA AAGATGGACT 
TTACAGCCAG TTCCCAGACA GAGTGGAGAT CGATTCATAG AAGAGAAGAC CTTGCTGTTG 
GCGGTGCGCT CCTATGTGTT TTTCTCCCAG CTCAGTGCGT GGCTCAGTGC CTCTCACGGA 
ATTGTCCCAA GAAACATCCT TTACAGAATA AGTGCAGCTG ATGAAGAACT GATATGGAAC 
TTCTCACAAA CACCCTCTGA ACATGCTTTC CCTGTTCCAA ATGTGTCACA TAGTGTCGCG 
CTGAAAGTGC GGGTTCAGTC TTTGCCTCGC CAGCCTAGAT ACCCTGTACT CAAATGTAGC 
ATCCACTCAG GTCTAGCTTT TTTGGGTAAG AGAGCACTAG AGCATGGCGA GGGTGGCAAC 
CAAGCAGGAG ACAACCGCAG TTCCCTTCGT CTGCCAAGAT CTCCACTTTT CTCCAGGTCT 
TTACACCCTT CTCCACCTTC CCACAGCCCT CTTAACACCC GTAAGTGTCC CCCTCGTCCC 
GAGTCCCCTC TTCCACCAGG CAAAGCTGTC AAATGGCTGT ACTCTCGGCT AAATGGCGGT 
ATAGACACCC CGCCTTCAGA ACCATATAGC TTGTGTACTA ATGGGGCAGA GAGCCCAAAG 
ACTTCAAGAA CCGAATCACC AATTCGTGGT TTTAAATCAC TCTCAATCAC AGACCCATTA 
GTGATCCCAA GCCCCAGCTC CATCTCAGGT GAAACCAACC CCCTTATTGG CTCCTTGCTT 
CAGGAAAGAC AGGAAGTCAT TGCTCGAATT GCTCAGCGAC TTAATTTCTG CGACCCTACA 
GCTCCCCATC TCCCTGATGC TCTCTTCACT TCTCAAGAAC CCCCAGGACA CAAAACCACC 
TGGAACTCGA CACAGGATAA GGAGTGTTTA AAGAAATCCA AAGATTCCCT CTTCTCAGTC 
CCTCATCCTC AAAATCACAA CGGAAACAGT CTAGAAATCC CTGAGAGGAG CAGGTCTTCT 
CTCTTCGACA CCCCCTTGAG CCCTAGAACC AGAACTCGGC TGGATAGAGT TGACCGTGAA 
TCAAAAACTT CCCCAAAACC CGCAACATGT AGGCGCTTGG TACTTAGTGA CCAGTCTGCA 
GAGGGTTCTC TAATTGCAGA TGCAGTTCAG GACATCTCCA GGTTGATTCA GGAGCGACTG 
CAACATTCTT ACAGTCTCCT GAATGGCACC TATAAATTAA AGACTTCTCA GAATGAGCAG 
GTTGGCTCAA ATCATGGAGC CCAAACAAAT GGCTTTGTAT CACTGTCAAG CCATAAGAAG 
CCTACAAATG CACCCAATGG TGAGGAAAGC ACAGATCCTC ATATTTCTCA TGCAACAAAA 
TGTTGCAGAT CTCCTGACTC TAGTCGTAGA AAGCCAGACT GTTCCCCTAG ACCACTAAAA 
GTGGCCAGTT TAAAACTTGA AGACCACAGC GTCACAAAAT CACAGCCTTT AACTGCAAGT 
AATCACCAAC ACTATGTCAG TAGAGAGTCC TGGACCTCCT TGAAAAACAA CTCTAGCCAT 
GCAAGTTCTC CTCAGGAGAA TGGACTGACC CAAATTGGAT ACCATCAGCC CTTCAAGAAC 
CGAGTGGCAA TCAGTGAAAA AGAAGCAGAA AAACACGTCC GGGACGGGTC CACTTGTCTT 
GAGAAAGATG AGAACCAAGA ACCCCATTCA TCCCTTTCTA GCACACCAGC CAACCTTACT 
TGCAACATCT CCAGTCTTGC CCCCACAGAG AGCAATCAAA CACAGAGATG CTCCCCCACC 
CCTTTGAAGT CATGCGGTAA CTGGAAGAAA CAGACTCGTC ACTCCATAGA TGGCACCGCA 
ACAAAAGCCT TCCACCCCTG CACAGGCCTA CCTCTCCTCT CCAGTCCTGT TCCTCAAAGA 
AAAAGCCAGA CAGGATATTT TGATTTAGAC ACGTCTCTGA TTCATTGCAG AGGTGTACCA 
TGGGCTGCCA ATAGGAGGGT TTTGAAAAGA TCACAAGACT ATGATGAGTC TCAGCATCAG 
ATTCTCAGTG CCAGCGCTCC ACCCGCCAAC CTCAGCCTTT TGGGTAACTT TGAGGAGTGT 
GTTCTGAATT ACCGTCTGGA GCCGCTGGGT ACTGTTGAGG GTTTTACAGC AGAAGTGGGC 
GCCAGCGGGA CCTTCTGTCC TAGTCACATG ACCTTACCTG TGGACGTGTC ATTCTACAGT 
GTCTCTGACG ATAACGCACC TTCCCCATAC ATGGGTGTCA TAAACTTGGA GTCACTCGGT 
AAAAGGGGAT ATCGAGTACC TCCTTCAGGA ACCATTCAAG TGACCTTATT TAACCCCAAT 
AAGACGGTGG TGAAGATGTT TGTGGTGATG TACGATCTGA GAGATATGCC TGCCAGCCAT 
CAGACATTCC TGCGGCAGAG AACCTTTTCA GTCCCAGTCA AACGGGAGTT CAACGGACAG 
AGCAACAAAA AGACGTCACT GGGGCAAGGA CGAACCCTCC GCTACCTCGT CCATCTGAGG 
TTCCAGAGTT CCAAATCTGG AAAGATCTAC CTCCATCGGG ACATCCGGCT CCTTTTTTCC 
CGTAAATCCA TGGAAGTGGA CAGTGGTGCT GCCTATGAGC TCAAGTCATT CACAGAGTTA 
CCTGCCGACC CTCCTTTCTC TCCCAGATGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam214a ( XM_009293780.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292055.1 Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42192
Clone ID Related Accession (Same CDS sequence)	XM_009293780.3 , XM_009293780.2
Accession Version	XM_009293780.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM214A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATTGTCAC 
CATAGAAGAG CGCTTCATCA ACCAGATGCC GCTGAGGAGT TCTTTGAATA TGATGCTGAG 
GAGTTTCTGG TGTTCCTGAC ACTTTTGATC ACAGAAGGAC GGACTCCAGA ATGCTCAGTA 
AAGGGGCGGA CGGAGGGCGT TCACTGCCCC CCGGCTCAGT CAGCTATGCC GGTCCTCACC 
AAACATGAAT GCAGTGACAA AATACCACAG TGTCGTCAGG CAAGGAGAAC AAGATCAGAA 
GTCATGCTTC TGTGGCGCAA TCACATCCCT ATAATGATCG AGGTGATGCT GCTTCCTGAT 
TGTTGCTATA GTGATGAAGG CCCAACCACA GACTGCACAG ACCTAAATGA CCCAGCAATA 
AAGCAAGATG CACTCTTACT AGAAAGATGG ACTTTACAGC CAGTTCCCAG ACAGAGTGGA 
GATCGATTCA TAGAAGAGAA GACCTTGCTG TTGGCGGTGC GCTCCTATGT GTTTTTCTCC 
CAGCTCAGTG CGTGGCTCAG TGCCTCTCAC GGAATTGTCC CAAGAAACAT CCTTTACAGA 
ATAAGTGCAG CTGATGAAGA ACTGATATGG AACTTCTCAC AAACACCCTC TGAACATGCT 
TTCCCTGTTC CAAATGTGTC ACATAGTGTC GCGCTGAAAG TGCGGGTTCA GTCTTTGCCT 
CGCCAGCCTA GATACCCTGT ACTCAAATGT AGCATCCACT CAGGTCTAGC TTTTTTGGGT 
AAGAGAGCAC TAGAGCATGG CGAGGGTGGC AACCAAGCAG GAGACAACCG CAGTTCCCTT 
CGTCTGCCAA GATCTCCACT TTTCTCCAGG TCTTTACACC CTTCTCCACC TTCCCACAGC 
CCTCTTAACA CCCGTAAGTG TCCCCCTCGT CCCGAGTCCC CTCTTCCACC AGGCAAAGCT 
GTCAAATGGC TGTACTCTCG GCTAAATGGC GGTATAGACA CCCCGCCTTC AGAACCATAT 
AGCTTGTGTA CTAATGGGGC AGAGAGCCCA AAGACTTCAA GAACCGAATC ACCAATTCGT 
GGTTTTAAAT CACTCTCAAT CACAGACCCA TTAGTGATCC CAAGCCCCAG CTCCATCTCA 
GGTGAAACCA ACCCCCTTAT TGGCTCCTTG CTTCAGGAAA GACAGGAAGT CATTGCTCGA 
ATTGCTCAGC GACTTAATTT CTGCGACCCT ACAGCTCCCC ATCTCCCTGA TGCTCTCTTC 
ACTTCTCAAG AACCCCCAGG ACACAAAACC ACCTGGAACT CGACACAGGA TAAGGAGTGT 
TTAAAGAAAT CCAAAGATTC CCTCTTCTCA GTCCCTCATC CTCAAAATCA CAACGGAAAC 
AGTCTAGAAA TCCCTGAGAG GAGCAGGTCT TCTCTCTTCG ACACCCCCTT GAGCCCTAGA 
ACCAGAACTC GGCTGGATAG AGTTGACCGT GAATCAAAAA CTTCCCCAAA ACCCGCAACA 
TGTAGGCGCT TGGTACTTAG TGACCAGTCT GCAGAGGGTT CTCTAATTGC AGATGCAGTT 
CAGGACATCT CCAGGTTGAT TCAGGAGCGA CTGCAACATT CTTACAGTCT CCTGAATGGC 
ACCTATAAAT TAAAGACTTC TCAGAATGAG CAGGTTGGCT CAAATCATGG AGCCCAAACA 
AATGGCTTTG TATCACTGTC AAGCCATAAG AAGCCTACAA ATGCACCCAA TGGTGAGGAA 
AGCACAGATC CTCATATTTC TCATGCAACA AAATGTTGCA GATCTCCTGA CTCTAGTCGT 
AGAAAGCCAG ACTGTTCCCC TAGACCACTA AAAGTGGCCA GTTTAAAACT TGAAGACCAC 
AGCGTCACAA AATCACAGCC TTTAACTGCA AGTAATCACC AACACTATGT CAGTAGAGAG 
TCCTGGACCT CCTTGAAAAA CAACTCTAGC CATGCAAGTT CTCCTCAGGA GAATGGACTG 
ACCCAAATTG GATACCATCA GCCCTTCAAG AACCGAGTGG CAATCAGTGA AAAAGAAGCA 
GAAAAACACG TCCGGGACGG GTCCACTTGT CTTGAGAAAG ATGAGAACCA AGAACCCCAT 
TCATCCCTTT CTAGCACACC AGCCAACCTT ACTTGCAACA TCTCCAGTCT TGCCCCCACA 
GAGAGCAATC AAACACAGAG ATGCTCCCCC ACCCCTTTGA AGTCATGCGG TAACTGGAAG 
AAACAGACTC GTCACTCCAT AGATGGCACC GCAACAAAAG CCTTCCACCC CTGCACAGGC 
CTACCTCTCC TCTCCAGTCC TGTTCCTCAA AGAAAAAGCC AGACAGGATA TTTTGATTTA 
GACACGTCTC TGATTCATTG CAGAGGTGTA CCATGGGCTG CCAATAGGAG GGTTTTGAAA 
AGATCACAAG ACTATGATGA GTCTCAGCAT CAGATTCTCA GTGCCAGCGC TCCACCCGCC 
AACCTCAGCC TTTTGGGTAA CTTTGAGGAG TGTGTTCTGA ATTACCGTCT GGAGCCGCTG 
GGTACTGTTG AGGGTTTTAC AGCAGAAGTG GGCGCCAGCG GGACCTTCTG TCCTAGTCAC 
ATGACCTTAC CTGTGGACGT GTCATTCTAC AGTGTCTCTG ACGATAACGC ACCTTCCCCA 
TACATGGGTG TCATAAACTT GGAGTCACTC GGTAAAAGGG GATATCGAGT ACCTCCTTCA 
GGAACCATTC AAGTGACCTT ATTTAACCCC AATAAGACGG TGGTGAAGAT GTTTGTGGTG 
ATGTACGATC TGAGAGATAT GCCTGCCAGC CATCAGACAT TCCTGCGGCA GAGAACCTTT 
TCAGTCCCAG TCAAACGGGA GTTCAACGGA CAGAGCAACA AAAAGACGTC ACTGGGGCAA 
GGACGAACCC TCCGCTACCT CGTCCATCTG AGGTTCCAGA GTTCCAAATC TGGAAAGATC 
TACCTCCATC GGGACATCCG GCTCCTTTTT TCCCGTAAAT CCATGGAAGT GGACAGTGGT 
GCTGCCTATG AGCTCAAGTC ATTCACAGAG TTACCTGCCG ACCCTCCTTT CTCTCCCAGA 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292055.1
CDS	734..3859
Translation	MSKGRVFKSKQRSEDGRRTAGILEGITVSVTNMKPDRDCHHRRALHQPDAAEEFFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSAMPVLTKHECSDKIPQCRQARRTRSEVMLLWRNHIPIMIEVMLLPDCCYSDEGPTTDCTDLNDPAIKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEELIWNFSQTPSEHAFPVPNVSHSVALKVRVQSLPRQPRYPVLKCSIHSGLAFLGKRALEHGEGGNQAGDNRSSLRLPRSPLFSRSLHPSPPSHSPLNTRKCPPRPESPLPPGKAVKWLYSRLNGGIDTPPSEPYSLCTNGAESPKTSRTESPIRGFKSLSITDPLVIPSPSSISGETNPLIGSLLQERQEVIARIAQRLNFCDPTAPHLPDALFTSQEPPGHKTTWNSTQDKECLKKSKDSLFSVPHPQNHNGNSLEIPERSRSSLFDTPLSPRTRTRLDRVDRESKTSPKPATCRRLVLSDQSAEGSLIADAVQDISRLIQERLQHSYSLLNGTYKLKTSQNEQVGSNHGAQTNGFVSLSSHKKPTNAPNGEESTDPHISHATKCCRSPDSSRRKPDCSPRPLKVASLKLEDHSVTKSQPLTASNHQHYVSRESWTSLKNNSSHASSPQENGLTQIGYHQPFKNRVAISEKEAEKHVRDGSTCLEKDENQEPHSSLSSTPANLTCNISSLAPTESNQTQRCSPTPLKSCGNWKKQTRHSIDGTATKAFHPCTGLPLLSSPVPQRKSQTGYFDLDTSLIHCRGVPWAANRRVLKRSQDYDESQHQILSASAPPANLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGTFCPSHMTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVMYDLRDMPASHQTFLRQRTFSVPVKREFNGQSNKKTSLGQGRTLRYLVHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSFTELPADPPFSPRC

Target ORF information:

RefSeq Version	XM_009293780.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293780.3

ORF Insert Sequence:

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATTGTCAC 
CATAGAAGAG CGCTTCATCA ACCAGATGCC GCTGAGGAGT TCTTTGAATA TGATGCTGAG 
GAGTTTCTGG TGTTCCTGAC ACTTTTGATC ACAGAAGGAC GGACTCCAGA ATGCTCAGTA 
AAGGGGCGGA CGGAGGGCGT TCACTGCCCC CCGGCTCAGT CAGCTATGCC GGTCCTCACC 
AAACATGAAT GCAGTGACAA AATACCACAG TGTCGTCAGG CAAGGAGAAC AAGATCAGAA 
GTCATGCTTC TGTGGCGCAA TCACATCCCT ATAATGATCG AGGTGATGCT GCTTCCTGAT 
TGTTGCTATA GTGATGAAGG CCCAACCACA GACTGCACAG ACCTAAATGA CCCAGCAATA 
AAGCAAGATG CACTCTTACT AGAAAGATGG ACTTTACAGC CAGTTCCCAG ACAGAGTGGA 
GATCGATTCA TAGAAGAGAA GACCTTGCTG TTGGCGGTGC GCTCCTATGT GTTTTTCTCC 
CAGCTCAGTG CGTGGCTCAG TGCCTCTCAC GGAATTGTCC CAAGAAACAT CCTTTACAGA 
ATAAGTGCAG CTGATGAAGA ACTGATATGG AACTTCTCAC AAACACCCTC TGAACATGCT 
TTCCCTGTTC CAAATGTGTC ACATAGTGTC GCGCTGAAAG TGCGGGTTCA GTCTTTGCCT 
CGCCAGCCTA GATACCCTGT ACTCAAATGT AGCATCCACT CAGGTCTAGC TTTTTTGGGT 
AAGAGAGCAC TAGAGCATGG CGAGGGTGGC AACCAAGCAG GAGACAACCG CAGTTCCCTT 
CGTCTGCCAA GATCTCCACT TTTCTCCAGG TCTTTACACC CTTCTCCACC TTCCCACAGC 
CCTCTTAACA CCCGTAAGTG TCCCCCTCGT CCCGAGTCCC CTCTTCCACC AGGCAAAGCT 
GTCAAATGGC TGTACTCTCG GCTAAATGGC GGTATAGACA CCCCGCCTTC AGAACCATAT 
AGCTTGTGTA CTAATGGGGC AGAGAGCCCA AAGACTTCAA GAACCGAATC ACCAATTCGT 
GGTTTTAAAT CACTCTCAAT CACAGACCCA TTAGTGATCC CAAGCCCCAG CTCCATCTCA 
GGTGAAACCA ACCCCCTTAT TGGCTCCTTG CTTCAGGAAA GACAGGAAGT CATTGCTCGA 
ATTGCTCAGC GACTTAATTT CTGCGACCCT ACAGCTCCCC ATCTCCCTGA TGCTCTCTTC 
ACTTCTCAAG AACCCCCAGG ACACAAAACC ACCTGGAACT CGACACAGGA TAAGGAGTGT 
TTAAAGAAAT CCAAAGATTC CCTCTTCTCA GTCCCTCATC CTCAAAATCA CAACGGAAAC 
AGTCTAGAAA TCCCTGAGAG GAGCAGGTCT TCTCTCTTCG ACACCCCCTT GAGCCCTAGA 
ACCAGAACTC GGCTGGATAG AGTTGACCGT GAATCAAAAA CTTCCCCAAA ACCCGCAACA 
TGTAGGCGCT TGGTACTTAG TGACCAGTCT GCAGAGGGTT CTCTAATTGC AGATGCAGTT 
CAGGACATCT CCAGGTTGAT TCAGGAGCGA CTGCAACATT CTTACAGTCT CCTGAATGGC 
ACCTATAAAT TAAAGACTTC TCAGAATGAG CAGGTTGGCT CAAATCATGG AGCCCAAACA 
AATGGCTTTG TATCACTGTC AAGCCATAAG AAGCCTACAA ATGCACCCAA TGGTGAGGAA 
AGCACAGATC CTCATATTTC TCATGCAACA AAATGTTGCA GATCTCCTGA CTCTAGTCGT 
AGAAAGCCAG ACTGTTCCCC TAGACCACTA AAAGTGGCCA GTTTAAAACT TGAAGACCAC 
AGCGTCACAA AATCACAGCC TTTAACTGCA AGTAATCACC AACACTATGT CAGTAGAGAG 
TCCTGGACCT CCTTGAAAAA CAACTCTAGC CATGCAAGTT CTCCTCAGGA GAATGGACTG 
ACCCAAATTG GATACCATCA GCCCTTCAAG AACCGAGTGG CAATCAGTGA AAAAGAAGCA 
GAAAAACACG TCCGGGACGG GTCCACTTGT CTTGAGAAAG ATGAGAACCA AGAACCCCAT 
TCATCCCTTT CTAGCACACC AGCCAACCTT ACTTGCAACA TCTCCAGTCT TGCCCCCACA 
GAGAGCAATC AAACACAGAG ATGCTCCCCC ACCCCTTTGA AGTCATGCGG TAACTGGAAG 
AAACAGACTC GTCACTCCAT AGATGGCACC GCAACAAAAG CCTTCCACCC CTGCACAGGC 
CTACCTCTCC TCTCCAGTCC TGTTCCTCAA AGAAAAAGCC AGACAGGATA TTTTGATTTA 
GACACGTCTC TGATTCATTG CAGAGGTGTA CCATGGGCTG CCAATAGGAG GGTTTTGAAA 
AGATCACAAG ACTATGATGA GTCTCAGCAT CAGATTCTCA GTGCCAGCGC TCCACCCGCC 
AACCTCAGCC TTTTGGGTAA CTTTGAGGAG TGTGTTCTGA ATTACCGTCT GGAGCCGCTG 
GGTACTGTTG AGGGTTTTAC AGCAGAAGTG GGCGCCAGCG GGACCTTCTG TCCTAGTCAC 
ATGACCTTAC CTGTGGACGT GTCATTCTAC AGTGTCTCTG ACGATAACGC ACCTTCCCCA 
TACATGGGTG TCATAAACTT GGAGTCACTC GGTAAAAGGG GATATCGAGT ACCTCCTTCA 
GGAACCATTC AAGTGACCTT ATTTAACCCC AATAAGACGG TGGTGAAGAT GTTTGTGGTG 
ATGTACGATC TGAGAGATAT GCCTGCCAGC CATCAGACAT TCCTGCGGCA GAGAACCTTT 
TCAGTCCCAG TCAAACGGGA GTTCAACGGA CAGAGCAACA AAAAGACGTC ACTGGGGCAA 
GGACGAACCC TCCGCTACCT CGTCCATCTG AGGTTCCAGA GTTCCAAATC TGGAAAGATC 
TACCTCCATC GGGACATCCG GCTCCTTTTT TCCCGTAAAT CCATGGAAGT GGACAGTGGT 
GCTGCCTATG AGCTCAAGTC ATTCACAGAG TTACCTGCCG ACCCTCCTTT CTCTCCCAGA 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam214a ( XM_009293780.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292055.1 Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X1, mRNA.

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CloneID	ODa42192
Clone ID Related Accession (Same CDS sequence)	XM_009293780.3 , XM_009293780.2
Accession Version	XM_009293780.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM214A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATTGTCAC 
CATAGAAGAG CGCTTCATCA ACCAGATGCC GCTGAGGAGT TCTTTGAATA TGATGCTGAG 
GAGTTTCTGG TGTTCCTGAC ACTTTTGATC ACAGAAGGAC GGACTCCAGA ATGCTCAGTA 
AAGGGGCGGA CGGAGGGCGT TCACTGCCCC CCGGCTCAGT CAGCTATGCC GGTCCTCACC 
AAACATGAAT GCAGTGACAA AATACCACAG TGTCGTCAGG CAAGGAGAAC AAGATCAGAA 
GTCATGCTTC TGTGGCGCAA TCACATCCCT ATAATGATCG AGGTGATGCT GCTTCCTGAT 
TGTTGCTATA GTGATGAAGG CCCAACCACA GACTGCACAG ACCTAAATGA CCCAGCAATA 
AAGCAAGATG CACTCTTACT AGAAAGATGG ACTTTACAGC CAGTTCCCAG ACAGAGTGGA 
GATCGATTCA TAGAAGAGAA GACCTTGCTG TTGGCGGTGC GCTCCTATGT GTTTTTCTCC 
CAGCTCAGTG CGTGGCTCAG TGCCTCTCAC GGAATTGTCC CAAGAAACAT CCTTTACAGA 
ATAAGTGCAG CTGATGAAGA ACTGATATGG AACTTCTCAC AAACACCCTC TGAACATGCT 
TTCCCTGTTC CAAATGTGTC ACATAGTGTC GCGCTGAAAG TGCGGGTTCA GTCTTTGCCT 
CGCCAGCCTA GATACCCTGT ACTCAAATGT AGCATCCACT CAGGTCTAGC TTTTTTGGGT 
AAGAGAGCAC TAGAGCATGG CGAGGGTGGC AACCAAGCAG GAGACAACCG CAGTTCCCTT 
CGTCTGCCAA GATCTCCACT TTTCTCCAGG TCTTTACACC CTTCTCCACC TTCCCACAGC 
CCTCTTAACA CCCGTAAGTG TCCCCCTCGT CCCGAGTCCC CTCTTCCACC AGGCAAAGCT 
GTCAAATGGC TGTACTCTCG GCTAAATGGC GGTATAGACA CCCCGCCTTC AGAACCATAT 
AGCTTGTGTA CTAATGGGGC AGAGAGCCCA AAGACTTCAA GAACCGAATC ACCAATTCGT 
GGTTTTAAAT CACTCTCAAT CACAGACCCA TTAGTGATCC CAAGCCCCAG CTCCATCTCA 
GGTGAAACCA ACCCCCTTAT TGGCTCCTTG CTTCAGGAAA GACAGGAAGT CATTGCTCGA 
ATTGCTCAGC GACTTAATTT CTGCGACCCT ACAGCTCCCC ATCTCCCTGA TGCTCTCTTC 
ACTTCTCAAG AACCCCCAGG ACACAAAACC ACCTGGAACT CGACACAGGA TAAGGAGTGT 
TTAAAGAAAT CCAAAGATTC CCTCTTCTCA GTCCCTCATC CTCAAAATCA CAACGGAAAC 
AGTCTAGAAA TCCCTGAGAG GAGCAGGTCT TCTCTCTTCG ACACCCCCTT GAGCCCTAGA 
ACCAGAACTC GGCTGGATAG AGTTGACCGT GAATCAAAAA CTTCCCCAAA ACCCGCAACA 
TGTAGGCGCT TGGTACTTAG TGACCAGTCT GCAGAGGGTT CTCTAATTGC AGATGCAGTT 
CAGGACATCT CCAGGTTGAT TCAGGAGCGA CTGCAACATT CTTACAGTCT CCTGAATGGC 
ACCTATAAAT TAAAGACTTC TCAGAATGAG CAGGTTGGCT CAAATCATGG AGCCCAAACA 
AATGGCTTTG TATCACTGTC AAGCCATAAG AAGCCTACAA ATGCACCCAA TGGTGAGGAA 
AGCACAGATC CTCATATTTC TCATGCAACA AAATGTTGCA GATCTCCTGA CTCTAGTCGT 
AGAAAGCCAG ACTGTTCCCC TAGACCACTA AAAGTGGCCA GTTTAAAACT TGAAGACCAC 
AGCGTCACAA AATCACAGCC TTTAACTGCA AGTAATCACC AACACTATGT CAGTAGAGAG 
TCCTGGACCT CCTTGAAAAA CAACTCTAGC CATGCAAGTT CTCCTCAGGA GAATGGACTG 
ACCCAAATTG GATACCATCA GCCCTTCAAG AACCGAGTGG CAATCAGTGA AAAAGAAGCA 
GAAAAACACG TCCGGGACGG GTCCACTTGT CTTGAGAAAG ATGAGAACCA AGAACCCCAT 
TCATCCCTTT CTAGCACACC AGCCAACCTT ACTTGCAACA TCTCCAGTCT TGCCCCCACA 
GAGAGCAATC AAACACAGAG ATGCTCCCCC ACCCCTTTGA AGTCATGCGG TAACTGGAAG 
AAACAGACTC GTCACTCCAT AGATGGCACC GCAACAAAAG CCTTCCACCC CTGCACAGGC 
CTACCTCTCC TCTCCAGTCC TGTTCCTCAA AGAAAAAGCC AGACAGGATA TTTTGATTTA 
GACACGTCTC TGATTCATTG CAGAGGTGTA CCATGGGCTG CCAATAGGAG GGTTTTGAAA 
AGATCACAAG ACTATGATGA GTCTCAGCAT CAGATTCTCA GTGCCAGCGC TCCACCCGCC 
AACCTCAGCC TTTTGGGTAA CTTTGAGGAG TGTGTTCTGA ATTACCGTCT GGAGCCGCTG 
GGTACTGTTG AGGGTTTTAC AGCAGAAGTG GGCGCCAGCG GGACCTTCTG TCCTAGTCAC 
ATGACCTTAC CTGTGGACGT GTCATTCTAC AGTGTCTCTG ACGATAACGC ACCTTCCCCA 
TACATGGGTG TCATAAACTT GGAGTCACTC GGTAAAAGGG GATATCGAGT ACCTCCTTCA 
GGAACCATTC AAGTGACCTT ATTTAACCCC AATAAGACGG TGGTGAAGAT GTTTGTGGTG 
ATGTACGATC TGAGAGATAT GCCTGCCAGC CATCAGACAT TCCTGCGGCA GAGAACCTTT 
TCAGTCCCAG TCAAACGGGA GTTCAACGGA CAGAGCAACA AAAAGACGTC ACTGGGGCAA 
GGACGAACCC TCCGCTACCT CGTCCATCTG AGGTTCCAGA GTTCCAAATC TGGAAAGATC 
TACCTCCATC GGGACATCCG GCTCCTTTTT TCCCGTAAAT CCATGGAAGT GGACAGTGGT 
GCTGCCTATG AGCTCAAGTC ATTCACAGAG TTACCTGCCG ACCCTCCTTT CTCTCCCAGA 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292055.1
CDS	714..3839
Translation	MSKGRVFKSKQRSEDGRRTAGILEGITVSVTNMKPDRDCHHRRALHQPDAAEEFFEYDAEEFLVFLTLLITEGRTPECSVKGRTEGVHCPPAQSAMPVLTKHECSDKIPQCRQARRTRSEVMLLWRNHIPIMIEVMLLPDCCYSDEGPTTDCTDLNDPAIKQDALLLERWTLQPVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEELIWNFSQTPSEHAFPVPNVSHSVALKVRVQSLPRQPRYPVLKCSIHSGLAFLGKRALEHGEGGNQAGDNRSSLRLPRSPLFSRSLHPSPPSHSPLNTRKCPPRPESPLPPGKAVKWLYSRLNGGIDTPPSEPYSLCTNGAESPKTSRTESPIRGFKSLSITDPLVIPSPSSISGETNPLIGSLLQERQEVIARIAQRLNFCDPTAPHLPDALFTSQEPPGHKTTWNSTQDKECLKKSKDSLFSVPHPQNHNGNSLEIPERSRSSLFDTPLSPRTRTRLDRVDRESKTSPKPATCRRLVLSDQSAEGSLIADAVQDISRLIQERLQHSYSLLNGTYKLKTSQNEQVGSNHGAQTNGFVSLSSHKKPTNAPNGEESTDPHISHATKCCRSPDSSRRKPDCSPRPLKVASLKLEDHSVTKSQPLTASNHQHYVSRESWTSLKNNSSHASSPQENGLTQIGYHQPFKNRVAISEKEAEKHVRDGSTCLEKDENQEPHSSLSSTPANLTCNISSLAPTESNQTQRCSPTPLKSCGNWKKQTRHSIDGTATKAFHPCTGLPLLSSPVPQRKSQTGYFDLDTSLIHCRGVPWAANRRVLKRSQDYDESQHQILSASAPPANLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGTFCPSHMTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPPSGTIQVTLFNPNKTVVKMFVVMYDLRDMPASHQTFLRQRTFSVPVKREFNGQSNKKTSLGQGRTLRYLVHLRFQSSKSGKIYLHRDIRLLFSRKSMEVDSGAAYELKSFTELPADPPFSPRC

Target ORF information:

RefSeq Version	XM_009293780.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 214, member A (fam214a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293780.2

ORF Insert Sequence:

ATGTCTAAGG GACGAGTTTT TAAGTCTAAA CAAAGATCTG AAGACGGAAG ACGGACCGCG 
GGCATCCTGG AGGGGATCAC GGTGTCTGTG ACCAACATGA AACCTGACAG AGATTGTCAC 
CATAGAAGAG CGCTTCATCA ACCAGATGCC GCTGAGGAGT TCTTTGAATA TGATGCTGAG 
GAGTTTCTGG TGTTCCTGAC ACTTTTGATC ACAGAAGGAC GGACTCCAGA ATGCTCAGTA 
AAGGGGCGGA CGGAGGGCGT TCACTGCCCC CCGGCTCAGT CAGCTATGCC GGTCCTCACC 
AAACATGAAT GCAGTGACAA AATACCACAG TGTCGTCAGG CAAGGAGAAC AAGATCAGAA 
GTCATGCTTC TGTGGCGCAA TCACATCCCT ATAATGATCG AGGTGATGCT GCTTCCTGAT 
TGTTGCTATA GTGATGAAGG CCCAACCACA GACTGCACAG ACCTAAATGA CCCAGCAATA 
AAGCAAGATG CACTCTTACT AGAAAGATGG ACTTTACAGC CAGTTCCCAG ACAGAGTGGA 
GATCGATTCA TAGAAGAGAA GACCTTGCTG TTGGCGGTGC GCTCCTATGT GTTTTTCTCC 
CAGCTCAGTG CGTGGCTCAG TGCCTCTCAC GGAATTGTCC CAAGAAACAT CCTTTACAGA 
ATAAGTGCAG CTGATGAAGA ACTGATATGG AACTTCTCAC AAACACCCTC TGAACATGCT 
TTCCCTGTTC CAAATGTGTC ACATAGTGTC GCGCTGAAAG TGCGGGTTCA GTCTTTGCCT 
CGCCAGCCTA GATACCCTGT ACTCAAATGT AGCATCCACT CAGGTCTAGC TTTTTTGGGT 
AAGAGAGCAC TAGAGCATGG CGAGGGTGGC AACCAAGCAG GAGACAACCG CAGTTCCCTT 
CGTCTGCCAA GATCTCCACT TTTCTCCAGG TCTTTACACC CTTCTCCACC TTCCCACAGC 
CCTCTTAACA CCCGTAAGTG TCCCCCTCGT CCCGAGTCCC CTCTTCCACC AGGCAAAGCT 
GTCAAATGGC TGTACTCTCG GCTAAATGGC GGTATAGACA CCCCGCCTTC AGAACCATAT 
AGCTTGTGTA CTAATGGGGC AGAGAGCCCA AAGACTTCAA GAACCGAATC ACCAATTCGT 
GGTTTTAAAT CACTCTCAAT CACAGACCCA TTAGTGATCC CAAGCCCCAG CTCCATCTCA 
GGTGAAACCA ACCCCCTTAT TGGCTCCTTG CTTCAGGAAA GACAGGAAGT CATTGCTCGA 
ATTGCTCAGC GACTTAATTT CTGCGACCCT ACAGCTCCCC ATCTCCCTGA TGCTCTCTTC 
ACTTCTCAAG AACCCCCAGG ACACAAAACC ACCTGGAACT CGACACAGGA TAAGGAGTGT 
TTAAAGAAAT CCAAAGATTC CCTCTTCTCA GTCCCTCATC CTCAAAATCA CAACGGAAAC 
AGTCTAGAAA TCCCTGAGAG GAGCAGGTCT TCTCTCTTCG ACACCCCCTT GAGCCCTAGA 
ACCAGAACTC GGCTGGATAG AGTTGACCGT GAATCAAAAA CTTCCCCAAA ACCCGCAACA 
TGTAGGCGCT TGGTACTTAG TGACCAGTCT GCAGAGGGTT CTCTAATTGC AGATGCAGTT 
CAGGACATCT CCAGGTTGAT TCAGGAGCGA CTGCAACATT CTTACAGTCT CCTGAATGGC 
ACCTATAAAT TAAAGACTTC TCAGAATGAG CAGGTTGGCT CAAATCATGG AGCCCAAACA 
AATGGCTTTG TATCACTGTC AAGCCATAAG AAGCCTACAA ATGCACCCAA TGGTGAGGAA 
AGCACAGATC CTCATATTTC TCATGCAACA AAATGTTGCA GATCTCCTGA CTCTAGTCGT 
AGAAAGCCAG ACTGTTCCCC TAGACCACTA AAAGTGGCCA GTTTAAAACT TGAAGACCAC 
AGCGTCACAA AATCACAGCC TTTAACTGCA AGTAATCACC AACACTATGT CAGTAGAGAG 
TCCTGGACCT CCTTGAAAAA CAACTCTAGC CATGCAAGTT CTCCTCAGGA GAATGGACTG 
ACCCAAATTG GATACCATCA GCCCTTCAAG AACCGAGTGG CAATCAGTGA AAAAGAAGCA 
GAAAAACACG TCCGGGACGG GTCCACTTGT CTTGAGAAAG ATGAGAACCA AGAACCCCAT 
TCATCCCTTT CTAGCACACC AGCCAACCTT ACTTGCAACA TCTCCAGTCT TGCCCCCACA 
GAGAGCAATC AAACACAGAG ATGCTCCCCC ACCCCTTTGA AGTCATGCGG TAACTGGAAG 
AAACAGACTC GTCACTCCAT AGATGGCACC GCAACAAAAG CCTTCCACCC CTGCACAGGC 
CTACCTCTCC TCTCCAGTCC TGTTCCTCAA AGAAAAAGCC AGACAGGATA TTTTGATTTA 
GACACGTCTC TGATTCATTG CAGAGGTGTA CCATGGGCTG CCAATAGGAG GGTTTTGAAA 
AGATCACAAG ACTATGATGA GTCTCAGCAT CAGATTCTCA GTGCCAGCGC TCCACCCGCC 
AACCTCAGCC TTTTGGGTAA CTTTGAGGAG TGTGTTCTGA ATTACCGTCT GGAGCCGCTG 
GGTACTGTTG AGGGTTTTAC AGCAGAAGTG GGCGCCAGCG GGACCTTCTG TCCTAGTCAC 
ATGACCTTAC CTGTGGACGT GTCATTCTAC AGTGTCTCTG ACGATAACGC ACCTTCCCCA 
TACATGGGTG TCATAAACTT GGAGTCACTC GGTAAAAGGG GATATCGAGT ACCTCCTTCA 
GGAACCATTC AAGTGACCTT ATTTAACCCC AATAAGACGG TGGTGAAGAT GTTTGTGGTG 
ATGTACGATC TGAGAGATAT GCCTGCCAGC CATCAGACAT TCCTGCGGCA GAGAACCTTT 
TCAGTCCCAG TCAAACGGGA GTTCAACGGA CAGAGCAACA AAAAGACGTC ACTGGGGCAA 
GGACGAACCC TCCGCTACCT CGTCCATCTG AGGTTCCAGA GTTCCAAATC TGGAAAGATC 
TACCTCCATC GGGACATCCG GCTCCTTTTT TCCCGTAAAT CCATGGAAGT GGACAGTGGT 
GCTGCCTATG AGCTCAAGTC ATTCACAGAG TTACCTGCCG ACCCTCCTTT CTCTCCCAGA 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

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fam214a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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