MYH11A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH11A
Entrez Gene ID	554168
Full Name	myosin, heavy chain 11a, smooth muscle
Synonyms	myh11
General protein information	Preferred Names myosin, heavy chain 11a, smooth muscle Names myosin-11 meltdown mlt myosin, heavy polypeptide 11, smooth muscle a smmhc smooth muscle myosin heavy chain
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024448.1	NP_001019619.1	myosin-11
XM_021476876.1	XP_021332551.1	myosin-11 isoform X2
XM_021476875.1	XP_021332550.1	myosin-11 isoform X1
XM_017356492.1	XP_017211981.1	myosin-11 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH11	NP_001035203.1
Danio rerio (zebrafish)	myh11a	NP_001019619.1
Xenopus tropicalis (tropical clawed frog)	LOC100490639	XP_002932251.1
Rattus norvegicus (Norway rat)	Myh11	NP_001164071.1
Pan troglodytes (chimpanzee)	LOC453949	XP_003952852.1
	MYH11	XP_001109463.2
Canis lupus familiaris (dog)	MYH11	XP_005621571.1
Bos taurus (cattle)	MYH11	NP_001095597.1
Mus musculus (house mouse)	Myh11	NP_001155247.1
Gallus gallus (chicken)	MYH11	NP_990605.1

Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution mRNA expression time course of embryonic development in zebrafish.
White RJ, Collins JE, Sealy IM, Wali N, Dooley CM, Digby Z, Stemple DL, Murphy DN, Billis K, Hourlier T, F?llgrabe A, Davis MP, Enright AJ, Busch-Nentwich EM
eLife6(2017 Nov)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

An exclusive cellular and molecular network governs intestinal smooth muscle cell differentiation in vertebrates.
Gays D, Hess C, Camporeale A, Ala U, Provero P, Mosimann C, Santoro MM
Development (Cambridge, England)144(3)464-478(2017 02)

Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
Chen WC, Wang Z, Missinato MA, Park DW, Long DW, Liu HJ, Zeng X, Yates NA, Kim K, Wang Y
Science advances2(11)e1600844(2016 Nov)

Graded effects of unregulated smooth muscle myosin on intestinal architecture, intestinal motility and vascular function in zebrafish.
Abrams J, Einhorn Z, Seiler C, Zong AB, Sweeney HL, Pack M
Disease models & mechanisms9(5)529-40(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

newly identified missense mutations in the switch-1 (S237Y) and coil-coiled (L1287M) domains of Myh11 fail to complement mlt Cell invasion was not detected in either homozygous mutant but could be induced by oxidative stress and activation of oncogenic signaling pathways.
Title: Graded effects of unregulated smooth muscle myosin on intestinal architecture, intestinal motility and vascular function in zebrafish.

The mlt mutation constitutively activates the Myh11 ATPase, which disrupts smooth muscle cells surrounding the posterior intestine.
Title: Mutation of smooth muscle myosin causes epithelial invasion and cystic expansion of the zebrafish intestine.

The following MYH11A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH11A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11474	XM_021476875.1 Latest version!	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa11475	XM_021476876.1 Latest version!	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59365	XM_017356492.1 Latest version!	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa03365	NM_001024448.1 Latest version!	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH11A ( XM_021476875.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021332550.1 Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11474
Clone ID Related Accession (Same CDS sequence)	XM_021476875.1
Accession Version	XM_021476875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin-11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTAATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCC GGCCTCATCT ACACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTCAA CAATCAGGAC AACAGTTTGC CTATGGTGAG 
CTGGAAAAGC AACTACTGCA GGCCAATCCC ATTCTAGAGG CCTTCGGTAA TGCCAAGACC 
ATCAAGAACG ATAACTCTTC CCGATTCGGC AAATTTATCC GCATTAACTT TGATGTGACA 
GGTTTCATAG TTGGAGCCAA CATCGAGACC TATCTGTTGG AGAAATCTCG CTGTATCAGA 
CAGGCGAAAA CAGAAAGAGC TTTCCACATC TTTTACTACA TGGTGGCAGG AACAAAGGAC 
AAACTACGTG AGGAGCTTTT GCTGGAAAAC TTCAACAACT ACCGTTTCCT CTCGGCTGGC 
CATGTCCAGA TTCCAGGCAA CCAGGATGAT GAGATGTATG ATGAAACTAT GGAGGCCATG 
GAGATCATGG GCTTCAGTGT TGAGGAAAGA GCAGATGTCC TCAAAGTAGT TTCAACTGTG 
CTGCAGTTGG GTAACATTGA GTTCAAGAAA GAGAGGAACC AAGAACAAGC TACCATGCCA 
GACAACACAG CTGCACAGAA AGTTTGCCAT CTTCAAGGCA TCAATGTGAC AGACTTCACA 
AGAGCCATTC TGACACCACG TATTAAAGTG GGTCGTGAGG TTGTGCAAAA GGCCCAAACC 
AAAGAACAGG CTGATTTTGC CATTGAAGCT TTAGCTAAAG CTATGTACGA ACGCTTGTTC 
CGTTGGATCC TTCTAAGAGT CAACAAAGCG TTAGACAAGA CGAAACGCCA GGGTGCATCT 
TTCCTGGGGA TCTTGGACAT CGCTGGCTTT GAGATATTTG AGAACAATTC CTTCGAGCAG 
CTCTGCATCA ACTACACCAA TGAGAAGCTT CAGCAGCTCT TTAACCACAC CATGTTCATC 
TTGGAGCAGG AGGAGTATCA GCGGGAAGGC ATCGAATGGA ACTTCATCGA CTTTGGGCTG 
GATTTACAAC CTTGCATTGA GCTTATTGAA AGACCGAACA ATCCTCCAGG CATCTTGGCT 
TTGCTGGATG AAGAATGTTG GTTTCCAAAG GCAACAGACG TGTCCTTTGT GGAGAAACTT 
TGCAACACTC ATGCTAATCA TACGAAGTTT GCAAAGCCTA AGCAACTGAA AGACAAGACT 
GAATTCTCTG TGCAACATTA TGCTGGACGG GTGGATTACA ATGCCGTAGC ATGGTTGACC 
AAAAACATGG ACCCTCTAAA TGACAACGTT ACAGCACTGC TGAATAATTC CTCCAATCCA 
TTTGTGCAAG ATCTGTGGAA AGATGCTGAT CGTGTGGTTG GATTGGAAAC CATTGCTAAG 
ATGTCGGACA GCCTTGCACC TAGTGCCTCT AAGACCAAGA AGGGCATGTT CCGTACTGTG 
GGCCAACTTT ACAAAGAGTC CCTGGCCAAG CTGATGACCA CACTGCACAA CACACAGCCA 
AACTTTGTCC GCTGTATTAT CCCCAACCAT GAGAAACGGG CAGGGAAGCT GGATGCCCAC 
CTGGTGCTAG AGCAGTTGAG ATGTAATGGT GTGCTAGAGG GCATTCGCAT CTGCCGCCAA 
GGATTTCCAA ACAGAATTGT CTTCCAGGAG TTCAGACAGC GATATGAGAT CCTGGCAGCG 
AATGCTATTC CAAAAGGCTT CATGGATGGC AAGCAGGCCT GCTGTCTGAT GATTAAGCAC 
CTGGACATTG ATCCAAACCT GTACCGAATT GGTCAGAGCA AAATCTTCTT CCGCACTGGA 
GTCCTGGCTC AGCTTGAAGA GGAGCGAGAC CTTAAGATCA CAGTCATCAT CATTGCCTTC 
CAGTCACAGG CCAGGGGATT CCTAGCCAGA AAGGCCTTTG CCAAGAGGCA GCAACAGCTA 
ACAGCTATGA AGGTGATCCA GAGGAACTGC GCTGCTTATC TGAAACTCAG GAACTGGCAG 
TGGTGGAGAC TCTTCACTAA GGTAAAGCCT CTCCTGCAGG TCACGCGCCA AGAGGAAGAG 
ATGAGCCTGA AAGAGGAGGA GCTCCAGAAG GCCAAAGAAT CTGCACAGAA GTTTGAAATC 
GAGCTAAAAG ACATTGCACT GAAGCACACA CAGCTTATGG ATGAAAGGAA CCAGCTCCAG 
GAGAAGCTGC AGGCAGAGAC GGAGCTCTAT GCGGAGGCAG AGGAGATGAG AGTACGGTTG 
GCAAGCAAGA AACAGGAACT GGAGGAGATC TTACATGAGA TGGAGGCCAG GTTGGAGGAA 
GAAGAGGATC GCGGCGCAGC CCTGCAAATG GAAAAGAAGA AAATGCACGA ACAGATTAAA 
GATCTTGAGG AGCATCTTGA GGAGGAAGAG GACGCTCGTC AGAAGCTGCA GCTGGAGAAG 
GTCACCTGTG ATGCAAAGAT CAAAAAGCTA GAGGATGATA TCCTGATAAT GGACGACCAA 
AACAACAAAC TGCAGAAGGA GAGGAAGATT CTAGAAGAAA GGATAGCAGA TTTTAGCTCA 
AACCTAGCAG AAGAAGAAGA AAAATCCAAA AACCTCACAA AGCTGAAGAA CAAGCACGAG 
TCAATGATCT CAGAACTGGA GGTGCGCTTA AAAAAAGAGG AGAAGACACG ACAGGAGTTG 
GATAAAGCCA AGAGGAAGTT GGAGGCGGAA TCCAATGACC TCCAGGAGCA GATCGCTGAC 
CTTCAAGCTC AGATTGCAGA TCTGAAAGCC CAGCTGGCTA AGAAAGAGGA GGAGCTACAG 
GCTGCATTGG CGCGACTGGA GGATGAGACA GGTCAGAAAA ACAATGCTCT GAAGAAGATC 
AGGGAGCTGG AGGGACACAT CTCGGATCTT CAAGAAGACC TGGAGTCAGA GCGGGCTGCG 
CGGAACAAGG CTGAAAAGAC TAAAAGAGAC TTGGGAGAGG AGCTTGAAGC TCTCAAATCA 
GAGCTGGAGG ACACACTGGA CACCACAGCC ACTCAGCAGG AGCTCAGGGC CAAACGTGAG 
CAGGAAGTGA CTCTTTTGAA GAGAGCTATA GAGGACGAGA GCCGCGTCCA TGAGGCTCAG 
GTGCAGGAGA TGAGACAGAA ACACACCCAA GCTTTGGAGG AGCTCACAGA GCAACTGGAG 
CAGTCCAAGA GGGTGAAGGT AAACCTGGAG AAAGCCAAGC AAGCTCTGGA GAAGGAGACG 
TCAGAACTCC ACGTGGAGCT CCGTTCTCTT ACTCAGGGCA AACAGGATGT GGAGCACAAG 
AAGAAGAAGC TGGAAGGACA GCTGGCTGAT CTGCAGTCTC GATTTAATGA CAGCGAGAGG 
CACAAAGCTG AGCTGGGAGA TCGGGTCTCT AAAATCACTG TGGAGCTGGA GAGCGTTACC 
AACCTTCTCA ATGAAGCTGA GGGAAAGAAC ATAAAGCTTA GCAAGGATGT GGCTAGCTTG 
AGTTCTCAAG TTCAAGACAC ACAGGAGTTG CTAGCCGAAG AAACAAGACA GAAGCTGCAA 
TTGTCTACCA AGCTACGCCA AATAGAGGAT GACCGCAACG CCCTTCAGGA GCAGCTTGAC 
GAGGAAGCAG AAGCCAAGAG AAATGTGGAG AGACATGTCT CAACACTTAA CATTCAGCTC 
TCTGACTTCA AGAAGAAGCT GGAAGAAATG ACGGGTAACG TGGAGCTTTT AGAGGAGGGT 
AAGAAGCGTC TGCAGAGAGA CCTGGAGGCA GCGAATACTC AGTTTGAGGA GAAGGCCGCA 
GCCTATGATA AGCTTGAGAA GACCAAGAAC AGACTGCAGC AGGAGCTTGA GGACACGCTG 
ATGGATTTGG ACAACCAGAG ACAGCTGGTG TCCAACCTTG AGAAGAAACA GAAGAAGTTT 
GATCAGATGC TTGCTGAAGA GAAGAGCATC TCTAGTAAGT ATGCAGATGA GCGAGATCGC 
GCAGAGGCCG AGGCTAGAGA GAAGGAGACC AAGGCTCTGT CTTTGGCCAG AGCTCTGGAG 
GAAGCTCAAG AAGCCCGCGA GGAGTTCGAG AGAGCCAATA AAGCCCTTCG TGCTGAAATG 
GAGGATCTAG TCAGCTCCAA AGACGACGTG GGCAAAAACG TGCATGAGCT TGAGAAGTCA 
AAACGTGGCT TGGAGGCTCA AGTCGAAGAG ATGAAGACAC AGCTGGAAGA GCTGGAAGAT 
GAGCTACAGG CTGCGGAAGA TGCCAAACTG CGTCTGGAAG TGAACATGCA GGCTCTGAAG 
GCTCAATTTG AGCGAGACCT CCAGGGCAGA GATGAGCAAG GAGAGGAAAA GAAGAGGCAG 
CTGGTCAAGC AGGTGCGTGA GCTGGAAACC GAACTTGAAG ATGAGCGCAA GCAAAGGACT 
GCACTTGCTG CTTCCAAGAA GAAGCTGGAG GGAGACCTAA AGGACCTTGA GGGACAGATT 
GAGACGTCTA ACAAGGGACG AGACGAGGCC ATCAAGCAGC TCCGAAAGCT CCAGGCTCAA 
ATGAAAGACT TCCAAAGAGA GCTGGATGAT GCTCATGCTG CCAGGGAAGA AGTTTTATCC 
AGTGCCAAGG AGAATGAGAG AAAGGCCAAG ACTCTGGAGG CTGAACTTCT ACAGCTGCAA 
GAGGACCTGG CCGCAGCTGA GAGGGCTAAA AAGCAAGTTG AAGCTGAGAG AGATGAACTG 
GCTGATGAGT TGGCCAGCAA TGCATCTGGA AAGTCTGCAC TGTCTGATGA GAAGAGACGG 
CTTGAAGCCA AGATTCAGCA GCTGGAGGAG GAACTGGAGG AGGAGCAAGG CAACATGGAG 
ATGCTCAATG ACAGGCTGAG AAAGAGCGCA CAGCAGGTCG ACCAGCTCAC CAACGAGCTG 
CAGGCCGAGC GCACCACATC CCAAAAGAAT GAGAGCGCCA GACAGCTGAT GGAGAGGCAG 
AATAAAGAGC TGAAGGCTAA ACTGCAGGAG ATGGAGAACC AAGTCAAATC CAAATTCAAG 
TCCTCCATCT CAGCTCTCGA AGCCAAAGTG GCACAACTGG AGGAGCAGCT TGAGCAGGAG 
AGCAGAGATA AGCAGAACAC AGCCAAAGCG GTTCGCCAGA AGGACAAGAA GCTGAAAGAG 
ATGATGACCC AAGTGGAGGA TGAACGAAAA CAAGCTGAGC AATATAAAGA CCAGGCGGAT 
AAGGCGACCG CTCGAGTCAA GCAGCTGAAG AGGCAGTTGG AGGAGAGCGA GGAAGAGTCT 
CAGAGAATCA CTGCGGCCCG CAGGAAACTG CAGCGAGAGC TGGACGAGGC CACCGAGACT 
AACGATGCCA TGAGCCGTGA GGTCAATTCC CTCAAGAGCA AACTCAGGCG AGGCAATGAG 
ACGTCATCCT TCAGCAGCAC ACCGAGACGT ACTGGAGGAG GAGGCCGACG GGGAATCATC 
GACAGCTCGG ATGCTGCGGA GGATGATGCC GACATGCAGA GCGATTACAA CGGAACCAAA 
TCTAATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332550.1
CDS	139..6090
Translation	MTKKGLSDDEKFLFTDKDFINSPVAQADWSAKKLVWVPSEKHGFESASIKEEHGDEVLVELMDNGKKITVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYYSGLIYTYSGLFCVVVNPYKMLPIYSEKIIEMYKGKKRHEVPPHIYSITDNAYRNMMQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDMSAQQSGQQFAYGELEKQLLQANPILEAFGNAKTIKNDNSSRFGKFIRINFDVTGFIVGANIETYLLEKSRCIRQAKTERAFHIFYYMVAGTKDKLREELLLENFNNYRFLSAGHVQIPGNQDDEMYDETMEAMEIMGFSVEERADVLKVVSTVLQLGNIEFKKERNQEQATMPDNTAAQKVCHLQGINVTDFTRAILTPRIKVGREVVQKAQTKEQADFAIEALAKAMYERLFRWILLRVNKALDKTKRQGASFLGILDIAGFEIFENNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGILALLDEECWFPKATDVSFVEKLCNTHANHTKFAKPKQLKDKTEFSVQHYAGRVDYNAVAWLTKNMDPLNDNVTALLNNSSNPFVQDLWKDADRVVGLETIAKMSDSLAPSASKTKKGMFRTVGQLYKESLAKLMTTLHNTQPNFVRCIIPNHEKRAGKLDAHLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACCLMIKHLDIDPNLYRIGQSKIFFRTGVLAQLEEERDLKITVIIIAFQSQARGFLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMSLKEEELQKAKESAQKFEIELKDIALKHTQLMDERNQLQEKLQAETELYAEAEEMRVRLASKKQELEEILHEMEARLEEEEDRGAALQMEKKKMHEQIKDLEEHLEEEEDARQKLQLEKVTCDAKIKKLEDDILIMDDQNNKLQKERKILEERIADFSSNLAEEEEKSKNLTKLKNKHESMISELEVRLKKEEKTRQELDKAKRKLEAESNDLQEQIADLQAQIADLKAQLAKKEEELQAALARLEDETGQKNNALKKIRELEGHISDLQEDLESERAARNKAEKTKRDLGEELEALKSELEDTLDTTATQQELRAKREQEVTLLKRAIEDESRVHEAQVQEMRQKHTQALEELTEQLEQSKRVKVNLEKAKQALEKETSELHVELRSLTQGKQDVEHKKKKLEGQLADLQSRFNDSERHKAELGDRVSKITVELESVTNLLNEAEGKNIKLSKDVASLSSQVQDTQELLAEETRQKLQLSTKLRQIEDDRNALQEQLDEEAEAKRNVERHVSTLNIQLSDFKKKLEEMTGNVELLEEGKKRLQRDLEAANTQFEEKAAAYDKLEKTKNRLQQELEDTLMDLDNQRQLVSNLEKKQKKFDQMLAEEKSISSKYADERDRAEAEAREKETKALSLARALEEAQEAREEFERANKALRAEMEDLVSSKDDVGKNVHELEKSKRGLEAQVEEMKTQLEELEDELQAAEDAKLRLEVNMQALKAQFERDLQGRDEQGEEKKRQLVKQVRELETELEDERKQRTALAASKKKLEGDLKDLEGQIETSNKGRDEAIKQLRKLQAQMKDFQRELDDAHAAREEVLSSAKENERKAKTLEAELLQLQEDLAAAERAKKQVEAERDELADELASNASGKSALSDEKRRLEAKIQQLEEELEEEQGNMEMLNDRLRKSAQQVDQLTNELQAERTTSQKNESARQLMERQNKELKAKLQEMENQVKSKFKSSISALEAKVAQLEEQLEQESRDKQNTAKAVRQKDKKLKEMMTQVEDERKQAEQYKDQADKATARVKQLKRQLEESEEESQRITAARRKLQRELDEATETNDAMSREVNSLKSKLRRGNETSSFSSTPRRTGGGGRRGIIDSSDAAEDDADMQSDYNGTKSNE

Target ORF information:

RefSeq Version	XM_021476875.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476875.1

ORF Insert Sequence:

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTAATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCC GGCCTCATCT ACACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTCAA CAATCAGGAC AACAGTTTGC CTATGGTGAG 
CTGGAAAAGC AACTACTGCA GGCCAATCCC ATTCTAGAGG CCTTCGGTAA TGCCAAGACC 
ATCAAGAACG ATAACTCTTC CCGATTCGGC AAATTTATCC GCATTAACTT TGATGTGACA 
GGTTTCATAG TTGGAGCCAA CATCGAGACC TATCTGTTGG AGAAATCTCG CTGTATCAGA 
CAGGCGAAAA CAGAAAGAGC TTTCCACATC TTTTACTACA TGGTGGCAGG AACAAAGGAC 
AAACTACGTG AGGAGCTTTT GCTGGAAAAC TTCAACAACT ACCGTTTCCT CTCGGCTGGC 
CATGTCCAGA TTCCAGGCAA CCAGGATGAT GAGATGTATG ATGAAACTAT GGAGGCCATG 
GAGATCATGG GCTTCAGTGT TGAGGAAAGA GCAGATGTCC TCAAAGTAGT TTCAACTGTG 
CTGCAGTTGG GTAACATTGA GTTCAAGAAA GAGAGGAACC AAGAACAAGC TACCATGCCA 
GACAACACAG CTGCACAGAA AGTTTGCCAT CTTCAAGGCA TCAATGTGAC AGACTTCACA 
AGAGCCATTC TGACACCACG TATTAAAGTG GGTCGTGAGG TTGTGCAAAA GGCCCAAACC 
AAAGAACAGG CTGATTTTGC CATTGAAGCT TTAGCTAAAG CTATGTACGA ACGCTTGTTC 
CGTTGGATCC TTCTAAGAGT CAACAAAGCG TTAGACAAGA CGAAACGCCA GGGTGCATCT 
TTCCTGGGGA TCTTGGACAT CGCTGGCTTT GAGATATTTG AGAACAATTC CTTCGAGCAG 
CTCTGCATCA ACTACACCAA TGAGAAGCTT CAGCAGCTCT TTAACCACAC CATGTTCATC 
TTGGAGCAGG AGGAGTATCA GCGGGAAGGC ATCGAATGGA ACTTCATCGA CTTTGGGCTG 
GATTTACAAC CTTGCATTGA GCTTATTGAA AGACCGAACA ATCCTCCAGG CATCTTGGCT 
TTGCTGGATG AAGAATGTTG GTTTCCAAAG GCAACAGACG TGTCCTTTGT GGAGAAACTT 
TGCAACACTC ATGCTAATCA TACGAAGTTT GCAAAGCCTA AGCAACTGAA AGACAAGACT 
GAATTCTCTG TGCAACATTA TGCTGGACGG GTGGATTACA ATGCCGTAGC ATGGTTGACC 
AAAAACATGG ACCCTCTAAA TGACAACGTT ACAGCACTGC TGAATAATTC CTCCAATCCA 
TTTGTGCAAG ATCTGTGGAA AGATGCTGAT CGTGTGGTTG GATTGGAAAC CATTGCTAAG 
ATGTCGGACA GCCTTGCACC TAGTGCCTCT AAGACCAAGA AGGGCATGTT CCGTACTGTG 
GGCCAACTTT ACAAAGAGTC CCTGGCCAAG CTGATGACCA CACTGCACAA CACACAGCCA 
AACTTTGTCC GCTGTATTAT CCCCAACCAT GAGAAACGGG CAGGGAAGCT GGATGCCCAC 
CTGGTGCTAG AGCAGTTGAG ATGTAATGGT GTGCTAGAGG GCATTCGCAT CTGCCGCCAA 
GGATTTCCAA ACAGAATTGT CTTCCAGGAG TTCAGACAGC GATATGAGAT CCTGGCAGCG 
AATGCTATTC CAAAAGGCTT CATGGATGGC AAGCAGGCCT GCTGTCTGAT GATTAAGCAC 
CTGGACATTG ATCCAAACCT GTACCGAATT GGTCAGAGCA AAATCTTCTT CCGCACTGGA 
GTCCTGGCTC AGCTTGAAGA GGAGCGAGAC CTTAAGATCA CAGTCATCAT CATTGCCTTC 
CAGTCACAGG CCAGGGGATT CCTAGCCAGA AAGGCCTTTG CCAAGAGGCA GCAACAGCTA 
ACAGCTATGA AGGTGATCCA GAGGAACTGC GCTGCTTATC TGAAACTCAG GAACTGGCAG 
TGGTGGAGAC TCTTCACTAA GGTAAAGCCT CTCCTGCAGG TCACGCGCCA AGAGGAAGAG 
ATGAGCCTGA AAGAGGAGGA GCTCCAGAAG GCCAAAGAAT CTGCACAGAA GTTTGAAATC 
GAGCTAAAAG ACATTGCACT GAAGCACACA CAGCTTATGG ATGAAAGGAA CCAGCTCCAG 
GAGAAGCTGC AGGCAGAGAC GGAGCTCTAT GCGGAGGCAG AGGAGATGAG AGTACGGTTG 
GCAAGCAAGA AACAGGAACT GGAGGAGATC TTACATGAGA TGGAGGCCAG GTTGGAGGAA 
GAAGAGGATC GCGGCGCAGC CCTGCAAATG GAAAAGAAGA AAATGCACGA ACAGATTAAA 
GATCTTGAGG AGCATCTTGA GGAGGAAGAG GACGCTCGTC AGAAGCTGCA GCTGGAGAAG 
GTCACCTGTG ATGCAAAGAT CAAAAAGCTA GAGGATGATA TCCTGATAAT GGACGACCAA 
AACAACAAAC TGCAGAAGGA GAGGAAGATT CTAGAAGAAA GGATAGCAGA TTTTAGCTCA 
AACCTAGCAG AAGAAGAAGA AAAATCCAAA AACCTCACAA AGCTGAAGAA CAAGCACGAG 
TCAATGATCT CAGAACTGGA GGTGCGCTTA AAAAAAGAGG AGAAGACACG ACAGGAGTTG 
GATAAAGCCA AGAGGAAGTT GGAGGCGGAA TCCAATGACC TCCAGGAGCA GATCGCTGAC 
CTTCAAGCTC AGATTGCAGA TCTGAAAGCC CAGCTGGCTA AGAAAGAGGA GGAGCTACAG 
GCTGCATTGG CGCGACTGGA GGATGAGACA GGTCAGAAAA ACAATGCTCT GAAGAAGATC 
AGGGAGCTGG AGGGACACAT CTCGGATCTT CAAGAAGACC TGGAGTCAGA GCGGGCTGCG 
CGGAACAAGG CTGAAAAGAC TAAAAGAGAC TTGGGAGAGG AGCTTGAAGC TCTCAAATCA 
GAGCTGGAGG ACACACTGGA CACCACAGCC ACTCAGCAGG AGCTCAGGGC CAAACGTGAG 
CAGGAAGTGA CTCTTTTGAA GAGAGCTATA GAGGACGAGA GCCGCGTCCA TGAGGCTCAG 
GTGCAGGAGA TGAGACAGAA ACACACCCAA GCTTTGGAGG AGCTCACAGA GCAACTGGAG 
CAGTCCAAGA GGGTGAAGGT AAACCTGGAG AAAGCCAAGC AAGCTCTGGA GAAGGAGACG 
TCAGAACTCC ACGTGGAGCT CCGTTCTCTT ACTCAGGGCA AACAGGATGT GGAGCACAAG 
AAGAAGAAGC TGGAAGGACA GCTGGCTGAT CTGCAGTCTC GATTTAATGA CAGCGAGAGG 
CACAAAGCTG AGCTGGGAGA TCGGGTCTCT AAAATCACTG TGGAGCTGGA GAGCGTTACC 
AACCTTCTCA ATGAAGCTGA GGGAAAGAAC ATAAAGCTTA GCAAGGATGT GGCTAGCTTG 
AGTTCTCAAG TTCAAGACAC ACAGGAGTTG CTAGCCGAAG AAACAAGACA GAAGCTGCAA 
TTGTCTACCA AGCTACGCCA AATAGAGGAT GACCGCAACG CCCTTCAGGA GCAGCTTGAC 
GAGGAAGCAG AAGCCAAGAG AAATGTGGAG AGACATGTCT CAACACTTAA CATTCAGCTC 
TCTGACTTCA AGAAGAAGCT GGAAGAAATG ACGGGTAACG TGGAGCTTTT AGAGGAGGGT 
AAGAAGCGTC TGCAGAGAGA CCTGGAGGCA GCGAATACTC AGTTTGAGGA GAAGGCCGCA 
GCCTATGATA AGCTTGAGAA GACCAAGAAC AGACTGCAGC AGGAGCTTGA GGACACGCTG 
ATGGATTTGG ACAACCAGAG ACAGCTGGTG TCCAACCTTG AGAAGAAACA GAAGAAGTTT 
GATCAGATGC TTGCTGAAGA GAAGAGCATC TCTAGTAAGT ATGCAGATGA GCGAGATCGC 
GCAGAGGCCG AGGCTAGAGA GAAGGAGACC AAGGCTCTGT CTTTGGCCAG AGCTCTGGAG 
GAAGCTCAAG AAGCCCGCGA GGAGTTCGAG AGAGCCAATA AAGCCCTTCG TGCTGAAATG 
GAGGATCTAG TCAGCTCCAA AGACGACGTG GGCAAAAACG TGCATGAGCT TGAGAAGTCA 
AAACGTGGCT TGGAGGCTCA AGTCGAAGAG ATGAAGACAC AGCTGGAAGA GCTGGAAGAT 
GAGCTACAGG CTGCGGAAGA TGCCAAACTG CGTCTGGAAG TGAACATGCA GGCTCTGAAG 
GCTCAATTTG AGCGAGACCT CCAGGGCAGA GATGAGCAAG GAGAGGAAAA GAAGAGGCAG 
CTGGTCAAGC AGGTGCGTGA GCTGGAAACC GAACTTGAAG ATGAGCGCAA GCAAAGGACT 
GCACTTGCTG CTTCCAAGAA GAAGCTGGAG GGAGACCTAA AGGACCTTGA GGGACAGATT 
GAGACGTCTA ACAAGGGACG AGACGAGGCC ATCAAGCAGC TCCGAAAGCT CCAGGCTCAA 
ATGAAAGACT TCCAAAGAGA GCTGGATGAT GCTCATGCTG CCAGGGAAGA AGTTTTATCC 
AGTGCCAAGG AGAATGAGAG AAAGGCCAAG ACTCTGGAGG CTGAACTTCT ACAGCTGCAA 
GAGGACCTGG CCGCAGCTGA GAGGGCTAAA AAGCAAGTTG AAGCTGAGAG AGATGAACTG 
GCTGATGAGT TGGCCAGCAA TGCATCTGGA AAGTCTGCAC TGTCTGATGA GAAGAGACGG 
CTTGAAGCCA AGATTCAGCA GCTGGAGGAG GAACTGGAGG AGGAGCAAGG CAACATGGAG 
ATGCTCAATG ACAGGCTGAG AAAGAGCGCA CAGCAGGTCG ACCAGCTCAC CAACGAGCTG 
CAGGCCGAGC GCACCACATC CCAAAAGAAT GAGAGCGCCA GACAGCTGAT GGAGAGGCAG 
AATAAAGAGC TGAAGGCTAA ACTGCAGGAG ATGGAGAACC AAGTCAAATC CAAATTCAAG 
TCCTCCATCT CAGCTCTCGA AGCCAAAGTG GCACAACTGG AGGAGCAGCT TGAGCAGGAG 
AGCAGAGATA AGCAGAACAC AGCCAAAGCG GTTCGCCAGA AGGACAAGAA GCTGAAAGAG 
ATGATGACCC AAGTGGAGGA TGAACGAAAA CAAGCTGAGC AATATAAAGA CCAGGCGGAT 
AAGGCGACCG CTCGAGTCAA GCAGCTGAAG AGGCAGTTGG AGGAGAGCGA GGAAGAGTCT 
CAGAGAATCA CTGCGGCCCG CAGGAAACTG CAGCGAGAGC TGGACGAGGC CACCGAGACT 
AACGATGCCA TGAGCCGTGA GGTCAATTCC CTCAAGAGCA AACTCAGGCG AGGCAATGAG 
ACGTCATCCT TCAGCAGCAC ACCGAGACGT ACTGGAGGAG GAGGCCGACG GGGAATCATC 
GACAGCTCGG ATGCTGCGGA GGATGATGCC GACATGCAGA GCGATTACAA CGGAACCAAA 
TCTAATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH11A ( XM_021476876.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021332551.1 Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X2, mRNA.

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CloneID	ODa11475
Clone ID Related Accession (Same CDS sequence)	XM_021476876.1
Accession Version	XM_021476876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin-11 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTAATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCC GGCCTCATCT ACACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTCAA CAATCAGGAC AACAGTTTGC CTATGGTGAG 
CTGGAAAAGC AACTACTGCA GGCCAATCCC ATTCTAGAGG CCTTCGGTAA TGCCAAGACC 
ATCAAGAACG ATAACTCTTC CCGATTCGGC AAATTTATCC GCATTAACTT TGATGTGACA 
GGTTTCATAG TTGGAGCCAA CATCGAGACC TATCTGTTGG AGAAATCTCG CTGTATCAGA 
CAGGCGAAAA CAGAAAGAGC TTTCCACATC TTTTACTACA TGGTGGCAGG AACAAAGGAC 
AAACTACGTG AGGAGCTTTT GCTGGAAAAC TTCAACAACT ACCGTTTCCT CTCGGCTGGC 
CATGTCCAGA TTCCAGGCAA CCAGGATGAT GAGATGTATG ATGAAACTAT GGAGGCCATG 
GAGATCATGG GCTTCAGTGT TGAGGAAAGA GCAGATGTCC TCAAAGTAGT TTCAACTGTG 
CTGCAGTTGG GTAACATTGA GTTCAAGAAA GAGAGGAACC AAGAACAAGC TACCATGCCA 
GACAACACAG CTGCACAGAA AGTTTGCCAT CTTCAAGGCA TCAATGTGAC AGACTTCACA 
AGAGCCATTC TGACACCACG TATTAAAGTG GGTCGTGAGG TTGTGCAAAA GGCCCAAACC 
AAAGAACAGG CTGATTTTGC CATTGAAGCT TTAGCTAAAG CTATGTACGA ACGCTTGTTC 
CGTTGGATCC TTCTAAGAGT CAACAAAGCG TTAGACAAGA CGAAACGCCA GGGTGCATCT 
TTCCTGGGGA TCTTGGACAT CGCTGGCTTT GAGATATTTG AGAACAATTC CTTCGAGCAG 
CTCTGCATCA ACTACACCAA TGAGAAGCTT CAGCAGCTCT TTAACCACAC CATGTTCATC 
TTGGAGCAGG AGGAGTATCA GCGGGAAGGC ATCGAATGGA ACTTCATCGA CTTTGGGCTG 
GATTTACAAC CTTGCATTGA GCTTATTGAA AGACCGAACA ATCCTCCAGG CATCTTGGCT 
TTGCTGGATG AAGAATGTTG GTTTCCAAAG GCAACAGACG TGTCCTTTGT GGAGAAACTT 
TGCAACACTC ATGCTAATCA TACGAAGTTT GCAAAGCCTA AGCAACTGAA AGACAAGACT 
GAATTCTCTG TGCAACATTA TGCTGGACGG GTGGATTACA ATGCCGTAGC ATGGTTGACC 
AAAAACATGG ACCCTCTAAA TGACAACGTT ACAGCACTGC TGAATAATTC CTCCAATCCA 
TTTGTGCAAG ATCTGTGGAA AGATGCTGAT CGTGTGGTTG GATTGGAAAC CATTGCTAAG 
ATGTCGGACA GCCTTGCACC TAGTGCCTCT AAGACCAAGA AGGGCATGTT CCGTACTGTG 
GGCCAACTTT ACAAAGAGTC CCTGGCCAAG CTGATGACCA CACTGCACAA CACACAGCCA 
AACTTTGTCC GCTGTATTAT CCCCAACCAT GAGAAACGGG CAGGGAAGCT GGATGCCCAC 
CTGGTGCTAG AGCAGTTGAG ATGTAATGGT GTGCTAGAGG GCATTCGCAT CTGCCGCCAA 
GGATTTCCAA ACAGAATTGT CTTCCAGGAG TTCAGACAGC GATATGAGAT CCTGGCAGCG 
AATGCTATTC CAAAAGGCTT CATGGATGGC AAGCAGGCCT GCTGTCTGAT GATTAAGCAC 
CTGGACATTG ATCCAAACCT GTACCGAATT GGTCAGAGCA AAATCTTCTT CCGCACTGGA 
GTCCTGGCTC AGCTTGAAGA GGAGCGAGAC CTTAAGATCA CAGTCATCAT CATTGCCTTC 
CAGTCACAGG CCAGGGGATT CCTAGCCAGA AAGGCCTTTG CCAAGAGGCA GCAACAGCTA 
ACAGCTATGA AGGTGATCCA GAGGAACTGC GCTGCTTATC TGAAACTCAG GAACTGGCAG 
TGGTGGAGAC TCTTCACTAA GGTAAAGCCT CTCCTGCAGG TCACGCGCCA AGAGGAAGAG 
ATGAGCCTGA AAGAGGAGGA GCTCCAGAAG GCCAAAGAAT CTGCACAGAA GTTTGAAATC 
GAGCTAAAAG ACATTGCACT GAAGCACACA CAGCTTATGG ATGAAAGGAA CCAGCTCCAG 
GAGAAGCTGC AGGCAGAGAC GGAGCTCTAT GCGGAGGCAG AGGAGATGAG AGTACGGTTG 
GCAAGCAAGA AACAGGAACT GGAGGAGATC TTACATGAGA TGGAGGCCAG GTTGGAGGAA 
GAAGAGGATC GCGGCGCAGC CCTGCAAATG GAAAAGAAGA AAATGCACGA ACAGATTAAA 
GATCTTGAGG AGCATCTTGA GGAGGAAGAG GACGCTCGTC AGAAGCTGCA GCTGGAGAAG 
GTCACCTGTG ATGCAAAGAT CAAAAAGCTA GAGGATGATA TCCTGATAAT GGACGACCAA 
AACAACAAAC TGCAGAAGGA GAGGAAGATT CTAGAAGAAA GGATAGCAGA TTTTAGCTCA 
AACCTAGCAG AAGAAGAAGA AAAATCCAAA AACCTCACAA AGCTGAAGAA CAAGCACGAG 
TCAATGATCT CAGAACTGGA GGTGCGCTTA AAAAAAGAGG AGAAGACACG ACAGGAGTTG 
GATAAAGCCA AGAGGAAGTT GGAGGCGGAA TCCAATGACC TCCAGGAGCA GATCGCTGAC 
CTTCAAGCTC AGATTGCAGA TCTGAAAGCC CAGCTGGCTA AGAAAGAGGA GGAGCTACAG 
GCTGCATTGG CGCGACTGGA GGATGAGACA GGTCAGAAAA ACAATGCTCT GAAGAAGATC 
AGGGAGCTGG AGGGACACAT CTCGGATCTT CAAGAAGACC TGGAGTCAGA GCGGGCTGCG 
CGGAACAAGG CTGAAAAGAC TAAAAGAGAC TTGGGAGAGG AGCTTGAAGC TCTCAAATCA 
GAGCTGGAGG ACACACTGGA CACCACAGCC ACTCAGCAGG AGCTCAGGGC CAAACGTGAG 
CAGGAAGTGA CTCTTTTGAA GAGAGCTATA GAGGACGAGA GCCGCGTCCA TGAGGCTCAG 
GTGCAGGAGA TGAGACAGAA ACACACCCAA GCTTTGGAGG AGCTCACAGA GCAACTGGAG 
CAGTCCAAGA GGGTGAAGGT AAACCTGGAG AAAGCCAAGC AAGCTCTGGA GAAGGAGACG 
TCAGAACTCC ACGTGGAGCT CCGTTCTCTT ACTCAGGGCA AACAGGATGT GGAGCACAAG 
AAGAAGAAGC TGGAAGGACA GCTGGCTGAT CTGCAGTCTC GATTTAATGA CAGCGAGAGG 
CACAAAGCTG AGCTGGGAGA TCGGGTCTCT AAAATCACTG TGGAGCTGGA GAGCGTTACC 
AACCTTCTCA ATGAAGCTGA GGGAAAGAAC ATAAAGCTTA GCAAGGATGT GGCTAGCTTG 
AGTTCTCAAG TTCAAGACAC ACAGGAGTTG CTAGCCGAAG AAACAAGACA GAAGCTGCAA 
TTGTCTACCA AGCTACGCCA AATAGAGGAT GACCGCAACG CCCTTCAGGA GCAGCTTGAC 
GAGGAAGCAG AAGCCAAGAG AAATGTGGAG AGACATGTCT CAACACTTAA CATTCAGCTC 
TCTGACTTCA AGAAGAAGCT GGAAGAAATG ACGGGTAACG TGGAGCTTTT AGAGGAGGGT 
AAGAAGCGTC TGCAGAGAGA CCTGGAGGCA GCGAATACTC AGTTTGAGGA GAAGGCCGCA 
GCCTATGATA AGCTTGAGAA GACCAAGAAC AGACTGCAGC AGGAGCTTGA GGACACGCTG 
ATGGATTTGG ACAACCAGAG ACAGCTGGTG TCCAACCTTG AGAAGAAACA GAAGAAGTTT 
GATCAGATGC TTGCTGAAGA GAAGAGCATC TCTAGTAAGT ATGCAGATGA GCGAGATCGC 
GCAGAGGCCG AGGCTAGAGA GAAGGAGACC AAGGCTCTGT CTTTGGCCAG AGCTCTGGAG 
GAAGCTCAAG AAGCCCGCGA GGAGTTCGAG AGAGCCAATA AAGCCCTTCG TGCTGAAATG 
GAGGATCTAG TCAGCTCCAA AGACGACGTG GGCAAAAACG TGCATGAGCT TGAGAAGTCA 
AAACGTGGCT TGGAGGCTCA AGTCGAAGAG ATGAAGACAC AGCTGGAAGA GCTGGAAGAT 
GAGCTACAGG CTGCGGAAGA TGCCAAACTG CGTCTGGAAG TGAACATGCA GGCTCTGAAG 
GCTCAATTTG AGCGAGACCT CCAGGGCAGA GATGAGCAAG GAGAGGAAAA GAAGAGGCAG 
CTGGTCAAGC AGGTGCGTGA GCTGGAAACC GAACTTGAAG ATGAGCGCAA GCAAAGGACT 
GCACTTGCTG CTTCCAAGAA GAAGCTGGAG GGAGACCTAA AGGACCTTGA GGGACAGATT 
GAGACGTCTA ACAAGGGACG AGACGAGGCC ATCAAGCAGC TCCGAAAGCT CCAGGCTCAA 
ATGAAAGACT TCCAAAGAGA GCTGGATGAT GCTCATGCTG CCAGGGAAGA AGTTTTATCC 
AGTGCCAAGG AGAATGAGAG AAAGGCCAAG ACTCTGGAGG CTGAACTTCT ACAGCTGCAA 
GAGGACCTGG CCGCAGCTGA GAGGGCTAAA AAGCAAGTTG AAGCTGAGAG AGATGAACTG 
GCTGATGAGT TGGCCAGCAA TGCATCTGGA AAGTCTGCAC TGTCTGATGA GAAGAGACGG 
CTTGAAGCCA AGATTCAGCA GCTGGAGGAG GAACTGGAGG AGGAGCAAGG CAACATGGAG 
ATGCTCAATG ACAGGCTGAG AAAGAGCGCA CAGCAGGTCG ACCAGCTCAC CAACGAGCTG 
CAGGCCGAGC GCACCACATC CCAAAAGAAT GAGAGCGCCA GACAGCTGAT GGAGAGGCAG 
AATAAAGAGC TGAAGGCTAA ACTGCAGGAG ATGGAGAACC AAGTCAAATC CAAATTCAAG 
TCCTCCATCT CAGCTCTCGA AGCCAAAGTG GCACAACTGG AGGAGCAGCT TGAGCAGGAG 
AGCAGAGATA AGCAGAACAC AGCCAAAGCG GTTCGCCAGA AGGACAAGAA GCTGAAAGAG 
ATGATGACCC AAGTGGAGGA TGAACGAAAA CAAGCTGAGC AATATAAAGA CCAGGCGGAT 
AAGGCGACCG CTCGAGTCAA GCAGCTGAAG AGGCAGTTGG AGGAGAGCGA GGAAGAGTCT 
CAGAGAATCA CTGCGGCCCG CAGGAAACTG CAGCGAGAGC TGGACGAGGC CACCGAGACT 
AACGATGCCA TGAGCCGTGA GGTCAATTCC CTCAAGAGCA AACTCAGAGG CAATCCTGAA 
CCCACACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332551.1
CDS	139..5970
Translation	MTKKGLSDDEKFLFTDKDFINSPVAQADWSAKKLVWVPSEKHGFESASIKEEHGDEVLVELMDNGKKITVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYYSGLIYTYSGLFCVVVNPYKMLPIYSEKIIEMYKGKKRHEVPPHIYSITDNAYRNMMQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDMSAQQSGQQFAYGELEKQLLQANPILEAFGNAKTIKNDNSSRFGKFIRINFDVTGFIVGANIETYLLEKSRCIRQAKTERAFHIFYYMVAGTKDKLREELLLENFNNYRFLSAGHVQIPGNQDDEMYDETMEAMEIMGFSVEERADVLKVVSTVLQLGNIEFKKERNQEQATMPDNTAAQKVCHLQGINVTDFTRAILTPRIKVGREVVQKAQTKEQADFAIEALAKAMYERLFRWILLRVNKALDKTKRQGASFLGILDIAGFEIFENNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGILALLDEECWFPKATDVSFVEKLCNTHANHTKFAKPKQLKDKTEFSVQHYAGRVDYNAVAWLTKNMDPLNDNVTALLNNSSNPFVQDLWKDADRVVGLETIAKMSDSLAPSASKTKKGMFRTVGQLYKESLAKLMTTLHNTQPNFVRCIIPNHEKRAGKLDAHLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACCLMIKHLDIDPNLYRIGQSKIFFRTGVLAQLEEERDLKITVIIIAFQSQARGFLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMSLKEEELQKAKESAQKFEIELKDIALKHTQLMDERNQLQEKLQAETELYAEAEEMRVRLASKKQELEEILHEMEARLEEEEDRGAALQMEKKKMHEQIKDLEEHLEEEEDARQKLQLEKVTCDAKIKKLEDDILIMDDQNNKLQKERKILEERIADFSSNLAEEEEKSKNLTKLKNKHESMISELEVRLKKEEKTRQELDKAKRKLEAESNDLQEQIADLQAQIADLKAQLAKKEEELQAALARLEDETGQKNNALKKIRELEGHISDLQEDLESERAARNKAEKTKRDLGEELEALKSELEDTLDTTATQQELRAKREQEVTLLKRAIEDESRVHEAQVQEMRQKHTQALEELTEQLEQSKRVKVNLEKAKQALEKETSELHVELRSLTQGKQDVEHKKKKLEGQLADLQSRFNDSERHKAELGDRVSKITVELESVTNLLNEAEGKNIKLSKDVASLSSQVQDTQELLAEETRQKLQLSTKLRQIEDDRNALQEQLDEEAEAKRNVERHVSTLNIQLSDFKKKLEEMTGNVELLEEGKKRLQRDLEAANTQFEEKAAAYDKLEKTKNRLQQELEDTLMDLDNQRQLVSNLEKKQKKFDQMLAEEKSISSKYADERDRAEAEAREKETKALSLARALEEAQEAREEFERANKALRAEMEDLVSSKDDVGKNVHELEKSKRGLEAQVEEMKTQLEELEDELQAAEDAKLRLEVNMQALKAQFERDLQGRDEQGEEKKRQLVKQVRELETELEDERKQRTALAASKKKLEGDLKDLEGQIETSNKGRDEAIKQLRKLQAQMKDFQRELDDAHAAREEVLSSAKENERKAKTLEAELLQLQEDLAAAERAKKQVEAERDELADELASNASGKSALSDEKRRLEAKIQQLEEELEEEQGNMEMLNDRLRKSAQQVDQLTNELQAERTTSQKNESARQLMERQNKELKAKLQEMENQVKSKFKSSISALEAKVAQLEEQLEQESRDKQNTAKAVRQKDKKLKEMMTQVEDERKQAEQYKDQADKATARVKQLKRQLEESEEESQRITAARRKLQRELDEATETNDAMSREVNSLKSKLRGNPEPTQ

Target ORF information:

RefSeq Version	XM_021476876.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476876.1

ORF Insert Sequence:

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTAATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCC GGCCTCATCT ACACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTCAA CAATCAGGAC AACAGTTTGC CTATGGTGAG 
CTGGAAAAGC AACTACTGCA GGCCAATCCC ATTCTAGAGG CCTTCGGTAA TGCCAAGACC 
ATCAAGAACG ATAACTCTTC CCGATTCGGC AAATTTATCC GCATTAACTT TGATGTGACA 
GGTTTCATAG TTGGAGCCAA CATCGAGACC TATCTGTTGG AGAAATCTCG CTGTATCAGA 
CAGGCGAAAA CAGAAAGAGC TTTCCACATC TTTTACTACA TGGTGGCAGG AACAAAGGAC 
AAACTACGTG AGGAGCTTTT GCTGGAAAAC TTCAACAACT ACCGTTTCCT CTCGGCTGGC 
CATGTCCAGA TTCCAGGCAA CCAGGATGAT GAGATGTATG ATGAAACTAT GGAGGCCATG 
GAGATCATGG GCTTCAGTGT TGAGGAAAGA GCAGATGTCC TCAAAGTAGT TTCAACTGTG 
CTGCAGTTGG GTAACATTGA GTTCAAGAAA GAGAGGAACC AAGAACAAGC TACCATGCCA 
GACAACACAG CTGCACAGAA AGTTTGCCAT CTTCAAGGCA TCAATGTGAC AGACTTCACA 
AGAGCCATTC TGACACCACG TATTAAAGTG GGTCGTGAGG TTGTGCAAAA GGCCCAAACC 
AAAGAACAGG CTGATTTTGC CATTGAAGCT TTAGCTAAAG CTATGTACGA ACGCTTGTTC 
CGTTGGATCC TTCTAAGAGT CAACAAAGCG TTAGACAAGA CGAAACGCCA GGGTGCATCT 
TTCCTGGGGA TCTTGGACAT CGCTGGCTTT GAGATATTTG AGAACAATTC CTTCGAGCAG 
CTCTGCATCA ACTACACCAA TGAGAAGCTT CAGCAGCTCT TTAACCACAC CATGTTCATC 
TTGGAGCAGG AGGAGTATCA GCGGGAAGGC ATCGAATGGA ACTTCATCGA CTTTGGGCTG 
GATTTACAAC CTTGCATTGA GCTTATTGAA AGACCGAACA ATCCTCCAGG CATCTTGGCT 
TTGCTGGATG AAGAATGTTG GTTTCCAAAG GCAACAGACG TGTCCTTTGT GGAGAAACTT 
TGCAACACTC ATGCTAATCA TACGAAGTTT GCAAAGCCTA AGCAACTGAA AGACAAGACT 
GAATTCTCTG TGCAACATTA TGCTGGACGG GTGGATTACA ATGCCGTAGC ATGGTTGACC 
AAAAACATGG ACCCTCTAAA TGACAACGTT ACAGCACTGC TGAATAATTC CTCCAATCCA 
TTTGTGCAAG ATCTGTGGAA AGATGCTGAT CGTGTGGTTG GATTGGAAAC CATTGCTAAG 
ATGTCGGACA GCCTTGCACC TAGTGCCTCT AAGACCAAGA AGGGCATGTT CCGTACTGTG 
GGCCAACTTT ACAAAGAGTC CCTGGCCAAG CTGATGACCA CACTGCACAA CACACAGCCA 
AACTTTGTCC GCTGTATTAT CCCCAACCAT GAGAAACGGG CAGGGAAGCT GGATGCCCAC 
CTGGTGCTAG AGCAGTTGAG ATGTAATGGT GTGCTAGAGG GCATTCGCAT CTGCCGCCAA 
GGATTTCCAA ACAGAATTGT CTTCCAGGAG TTCAGACAGC GATATGAGAT CCTGGCAGCG 
AATGCTATTC CAAAAGGCTT CATGGATGGC AAGCAGGCCT GCTGTCTGAT GATTAAGCAC 
CTGGACATTG ATCCAAACCT GTACCGAATT GGTCAGAGCA AAATCTTCTT CCGCACTGGA 
GTCCTGGCTC AGCTTGAAGA GGAGCGAGAC CTTAAGATCA CAGTCATCAT CATTGCCTTC 
CAGTCACAGG CCAGGGGATT CCTAGCCAGA AAGGCCTTTG CCAAGAGGCA GCAACAGCTA 
ACAGCTATGA AGGTGATCCA GAGGAACTGC GCTGCTTATC TGAAACTCAG GAACTGGCAG 
TGGTGGAGAC TCTTCACTAA GGTAAAGCCT CTCCTGCAGG TCACGCGCCA AGAGGAAGAG 
ATGAGCCTGA AAGAGGAGGA GCTCCAGAAG GCCAAAGAAT CTGCACAGAA GTTTGAAATC 
GAGCTAAAAG ACATTGCACT GAAGCACACA CAGCTTATGG ATGAAAGGAA CCAGCTCCAG 
GAGAAGCTGC AGGCAGAGAC GGAGCTCTAT GCGGAGGCAG AGGAGATGAG AGTACGGTTG 
GCAAGCAAGA AACAGGAACT GGAGGAGATC TTACATGAGA TGGAGGCCAG GTTGGAGGAA 
GAAGAGGATC GCGGCGCAGC CCTGCAAATG GAAAAGAAGA AAATGCACGA ACAGATTAAA 
GATCTTGAGG AGCATCTTGA GGAGGAAGAG GACGCTCGTC AGAAGCTGCA GCTGGAGAAG 
GTCACCTGTG ATGCAAAGAT CAAAAAGCTA GAGGATGATA TCCTGATAAT GGACGACCAA 
AACAACAAAC TGCAGAAGGA GAGGAAGATT CTAGAAGAAA GGATAGCAGA TTTTAGCTCA 
AACCTAGCAG AAGAAGAAGA AAAATCCAAA AACCTCACAA AGCTGAAGAA CAAGCACGAG 
TCAATGATCT CAGAACTGGA GGTGCGCTTA AAAAAAGAGG AGAAGACACG ACAGGAGTTG 
GATAAAGCCA AGAGGAAGTT GGAGGCGGAA TCCAATGACC TCCAGGAGCA GATCGCTGAC 
CTTCAAGCTC AGATTGCAGA TCTGAAAGCC CAGCTGGCTA AGAAAGAGGA GGAGCTACAG 
GCTGCATTGG CGCGACTGGA GGATGAGACA GGTCAGAAAA ACAATGCTCT GAAGAAGATC 
AGGGAGCTGG AGGGACACAT CTCGGATCTT CAAGAAGACC TGGAGTCAGA GCGGGCTGCG 
CGGAACAAGG CTGAAAAGAC TAAAAGAGAC TTGGGAGAGG AGCTTGAAGC TCTCAAATCA 
GAGCTGGAGG ACACACTGGA CACCACAGCC ACTCAGCAGG AGCTCAGGGC CAAACGTGAG 
CAGGAAGTGA CTCTTTTGAA GAGAGCTATA GAGGACGAGA GCCGCGTCCA TGAGGCTCAG 
GTGCAGGAGA TGAGACAGAA ACACACCCAA GCTTTGGAGG AGCTCACAGA GCAACTGGAG 
CAGTCCAAGA GGGTGAAGGT AAACCTGGAG AAAGCCAAGC AAGCTCTGGA GAAGGAGACG 
TCAGAACTCC ACGTGGAGCT CCGTTCTCTT ACTCAGGGCA AACAGGATGT GGAGCACAAG 
AAGAAGAAGC TGGAAGGACA GCTGGCTGAT CTGCAGTCTC GATTTAATGA CAGCGAGAGG 
CACAAAGCTG AGCTGGGAGA TCGGGTCTCT AAAATCACTG TGGAGCTGGA GAGCGTTACC 
AACCTTCTCA ATGAAGCTGA GGGAAAGAAC ATAAAGCTTA GCAAGGATGT GGCTAGCTTG 
AGTTCTCAAG TTCAAGACAC ACAGGAGTTG CTAGCCGAAG AAACAAGACA GAAGCTGCAA 
TTGTCTACCA AGCTACGCCA AATAGAGGAT GACCGCAACG CCCTTCAGGA GCAGCTTGAC 
GAGGAAGCAG AAGCCAAGAG AAATGTGGAG AGACATGTCT CAACACTTAA CATTCAGCTC 
TCTGACTTCA AGAAGAAGCT GGAAGAAATG ACGGGTAACG TGGAGCTTTT AGAGGAGGGT 
AAGAAGCGTC TGCAGAGAGA CCTGGAGGCA GCGAATACTC AGTTTGAGGA GAAGGCCGCA 
GCCTATGATA AGCTTGAGAA GACCAAGAAC AGACTGCAGC AGGAGCTTGA GGACACGCTG 
ATGGATTTGG ACAACCAGAG ACAGCTGGTG TCCAACCTTG AGAAGAAACA GAAGAAGTTT 
GATCAGATGC TTGCTGAAGA GAAGAGCATC TCTAGTAAGT ATGCAGATGA GCGAGATCGC 
GCAGAGGCCG AGGCTAGAGA GAAGGAGACC AAGGCTCTGT CTTTGGCCAG AGCTCTGGAG 
GAAGCTCAAG AAGCCCGCGA GGAGTTCGAG AGAGCCAATA AAGCCCTTCG TGCTGAAATG 
GAGGATCTAG TCAGCTCCAA AGACGACGTG GGCAAAAACG TGCATGAGCT TGAGAAGTCA 
AAACGTGGCT TGGAGGCTCA AGTCGAAGAG ATGAAGACAC AGCTGGAAGA GCTGGAAGAT 
GAGCTACAGG CTGCGGAAGA TGCCAAACTG CGTCTGGAAG TGAACATGCA GGCTCTGAAG 
GCTCAATTTG AGCGAGACCT CCAGGGCAGA GATGAGCAAG GAGAGGAAAA GAAGAGGCAG 
CTGGTCAAGC AGGTGCGTGA GCTGGAAACC GAACTTGAAG ATGAGCGCAA GCAAAGGACT 
GCACTTGCTG CTTCCAAGAA GAAGCTGGAG GGAGACCTAA AGGACCTTGA GGGACAGATT 
GAGACGTCTA ACAAGGGACG AGACGAGGCC ATCAAGCAGC TCCGAAAGCT CCAGGCTCAA 
ATGAAAGACT TCCAAAGAGA GCTGGATGAT GCTCATGCTG CCAGGGAAGA AGTTTTATCC 
AGTGCCAAGG AGAATGAGAG AAAGGCCAAG ACTCTGGAGG CTGAACTTCT ACAGCTGCAA 
GAGGACCTGG CCGCAGCTGA GAGGGCTAAA AAGCAAGTTG AAGCTGAGAG AGATGAACTG 
GCTGATGAGT TGGCCAGCAA TGCATCTGGA AAGTCTGCAC TGTCTGATGA GAAGAGACGG 
CTTGAAGCCA AGATTCAGCA GCTGGAGGAG GAACTGGAGG AGGAGCAAGG CAACATGGAG 
ATGCTCAATG ACAGGCTGAG AAAGAGCGCA CAGCAGGTCG ACCAGCTCAC CAACGAGCTG 
CAGGCCGAGC GCACCACATC CCAAAAGAAT GAGAGCGCCA GACAGCTGAT GGAGAGGCAG 
AATAAAGAGC TGAAGGCTAA ACTGCAGGAG ATGGAGAACC AAGTCAAATC CAAATTCAAG 
TCCTCCATCT CAGCTCTCGA AGCCAAAGTG GCACAACTGG AGGAGCAGCT TGAGCAGGAG 
AGCAGAGATA AGCAGAACAC AGCCAAAGCG GTTCGCCAGA AGGACAAGAA GCTGAAAGAG 
ATGATGACCC AAGTGGAGGA TGAACGAAAA CAAGCTGAGC AATATAAAGA CCAGGCGGAT 
AAGGCGACCG CTCGAGTCAA GCAGCTGAAG AGGCAGTTGG AGGAGAGCGA GGAAGAGTCT 
CAGAGAATCA CTGCGGCCCG CAGGAAACTG CAGCGAGAGC TGGACGAGGC CACCGAGACT 
AACGATGCCA TGAGCCGTGA GGTCAATTCC CTCAAGAGCA AACTCAGAGG CAATCCTGAA 
CCCACACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH11A ( XM_017356492.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211981.1 Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X1, mRNA.

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CloneID	ODa59365
Clone ID Related Accession (Same CDS sequence)	XM_017356492.1
Accession Version	XM_017356492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin-11 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTAATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCC GGCCTCATCT ACACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTGGT GAGCTGGAAA AGCAACTACT GCAGGCCAAT 
CCCATTCTAG AGGCCTTCGG TAATGCCAAG ACCATCAAGA ACGATAACTC TTCCCGATTC 
GGCAAATTTA TCCGCATTAA CTTTGATGTG ACAGGTTTCA TAGTTGGAGC CAACATCGAG 
ACCTATCTGT TGGAGAAATC TCGCTGTATC AGACAGGCGA AAACAGAAAG AGCTTTCCAC 
ATCTTTTACT ACATGGTGGC AGGAACAAAG GACAAACTAC GTGAGGAGCT TTTGCTGGAA 
AACTTCAACA ACTACCGTTT CCTCTCGGCT GGCCATGTCC AGATTCCAGG CAACCAGGAT 
GATGAGATGT ATGATGAAAC TATGGAGGCC ATGGAGATCA TGGGCTTCAG TGTTGAGGAA 
AGAGCAGATG TCCTCAAAGT AGTTTCAACT GTGCTGCAGT TGGGTAACAT TGAGTTCAAG 
AAAGAGAGGA ACCAAGAACA AGCTACCATG CCAGACAACA CAGCTGCACA GAAAGTTTGC 
CATCTTCAAG GCATCAATGT GACAGACTTC ACAAGAGCCA TTCTGACACC ACGTATTAAA 
GTGGGTCGTG AGGTTGTGCA AAAGGCCCAA ACCAAAGAAC AGGCTGATTT TGCCATTGAA 
GCTTTAGCTA AAGCTATGTA CGAACGCTTG TTCCGTTGGA TCCTTCTAAG AGTCAACAAA 
GCGTTAGACA AGACGAAACG CCAGGGTGCA TCTTTCCTGG GGATCTTGGA CATCGCTGGC 
TTTGAGATAT TTGAGAACAA TTCCTTCGAG CAGCTCTGCA TCAACTACAC CAATGAGAAG 
CTTCAGCAGC TCTTTAACCA CACCATGTTC ATCTTGGAGC AGGAGGAGTA TCAGCGGGAA 
GGCATCGAAT GGAACTTCAT CGACTTTGGG CTGGATTTAC AACCTTGCAT TGAGCTTATT 
GAAAGACCGA ACAATCCTCC AGGCATCTTG GCTTTGCTGG ATGAAGAATG TTGGTTTCCA 
AAGGCAACAG ACGTGTCCTT TGTGGAGAAA CTTTGCAACA CTCATGCTAA TCATACGAAG 
TTTGCAAAGC CTAAGCAACT GAAAGACAAG ACTGAATTCT CTGTGCAACA TTATGCTGGA 
CGGGTGGATT ACAATGCCGT AGCATGGTTG ACCAAAAACA TGGACCCTCT AAATGACAAC 
GTTACAGCAC TGCTGAATAA TTCCTCCAAT CCATTTGTGC AAGATCTGTG GAAAGATGCT 
GATCGTGTGG TTGGATTGGA AACCATTGCT AAGATGTCGG ACAGCCTTGC ACCTAGTGCC 
TCTAAGACCA AGAAGGGCAT GTTCCGTACT GTGGGCCAAC TTTACAAAGA GTCCCTGGCC 
AAGCTGATGA CCACACTGCA CAACACACAG CCAAACTTTG TCCGCTGTAT TATCCCCAAC 
CATGAGAAAC GGGCAGGGAA GCTGGATGCC CACCTGGTGC TAGAGCAGTT GAGATGTAAT 
GGTGTGCTAG AGGGCATTCG CATCTGCCGC CAAGGATTTC CAAACAGAAT TGTCTTCCAG 
GAGTTCAGAC AGCGATATGA GATCCTGGCA GCGAATGCTA TTCCAAAAGG CTTCATGGAT 
GGCAAGCAGG CCTGCTGTCT GATGATTAAG CACCTGGACA TTGATCCAAA CCTGTACCGA 
ATTGGTCAGA GCAAAATCTT CTTCCGCACT GGAGTCCTGG CTCAGCTTGA AGAGGAGCGA 
GACCTTAAGA TCACAGTCAT CATCATTGCC TTCCAGTCAC AGGCCAGGGG ATTCCTAGCC 
AGAAAGGCCT TTGCCAAGAG GCAGCAACAG CTAACAGCTA TGAAGGTGAT CCAGAGGAAC 
TGCGCTGCTT ATCTGAAACT CAGGAACTGG CAGTGGTGGA GACTCTTCAC TAAGGTAAAG 
CCTCTCCTGC AGGTCACGCG CCAAGAGGAA GAGATGAGCC TGAAAGAGGA GGAGCTCCAG 
AAGGCCAAAG AATCTGCACA GAAGTTTGAA ATCGAGCTAA AAGACATTGC ACTGAAGCAC 
ACACAGCTTA TGGATGAAAG GAACCAGCTC CAGGAGAAGC TGCAGGCAGA GACGGAGCTC 
TATGCGGAGG CAGAGGAGAT GAGAGTACGG TTGGCAAGCA AGAAACAGGA ACTGGAGGAG 
ATCTTACATG AGATGGAGGC CAGGTTGGAG GAAGAAGAGG ATCGCGGCGC AGCCCTGCAA 
ATGGAAAAGA AGAAAATGCA CGAACAGATT AAAGATCTTG AGGAGCATCT TGAGGAGGAA 
GAGGACGCTC GTCAGAAGCT GCAGCTGGAG AAGGTCACCT GTGATGCAAA GATCAAAAAG 
CTAGAGGATG ATATCCTGAT AATGGACGAC CAAAACAACA AACTGCAGAA GGAGAGGAAG 
ATTCTAGAAG AAAGGATAGC AGATTTTAGC TCAAACCTAG CAGAAGAAGA AGAAAAATCC 
AAAAACCTCA CAAAGCTGAA GAACAAGCAC GAGTCAATGA TCTCAGAACT GGAGGTGCGC 
TTAAAAAAAG AGGAGAAGAC ACGACAGGAG TTGGATAAAG CCAAGAGGAA GTTGGAGGCG 
GAATCCAATG ACCTCCAGGA GCAGATCGCT GACCTTCAAG CTCAGATTGC AGATCTGAAA 
GCCCAGCTGG CTAAGAAAGA GGAGGAGCTA CAGGCTGCAT TGGCGCGACT GGAGGATGAG 
ACAGGTCAGA AAAACAATGC TCTGAAGAAG ATCAGGGAGC TGGAGGGACA CATCTCGGAT 
CTTCAAGAAG ACCTGGAGTC AGAGCGGGCT GCGCGGAACA AGGCTGAAAA GACTAAAAGA 
GACTTGGGAG AGGAGCTTGA AGCTCTCAAA TCAGAGCTGG AGGACACACT GGACACCACA 
GCCACTCAGC AGGAGCTCAG GGCCAAACGT GAGCAGGAAG TGACTCTTTT GAAGAGAGCT 
ATAGAGGACG AGAGCCGCGT CCATGAGGCT CAGGTGCAGG AGATGAGACA GAAACACACC 
CAAGCTTTGG AGGAGCTCAC AGAGCAACTG GAGCAGTCCA AGAGGGTGAA GGTAAACCTG 
GAGAAAGCCA AGCAAGCTCT GGAGAAGGAG ACGTCAGAAC TCCACGTGGA GCTCCGTTCT 
CTTACTCAGG GCAAACAGGA TGTGGAGCAC AAGAAGAAGA AGCTGGAAGG ACAGCTGGCT 
GATCTGCAGT CTCGATTTAA TGACAGCGAG AGGCACAAAG CTGAGCTGGG AGATCGGGTC 
TCTAAAATCA CTGTGGAGCT GGAGAGCGTT ACCAACCTTC TCAATGAAGC TGAGGGAAAG 
AACATAAAGC TTAGCAAGGA TGTGGCTAGC TTGAGTTCTC AAGTTCAAGA CACACAGGAG 
TTGCTAGCCG AAGAAACAAG ACAGAAGCTG CAATTGTCTA CCAAGCTACG CCAAATAGAG 
GATGACCGCA ACGCCCTTCA GGAGCAGCTT GACGAGGAAG CAGAAGCCAA GAGAAATGTG 
GAGAGACATG TCTCAACACT TAACATTCAG CTCTCTGACT TCAAGAAGAA GCTGGAAGAA 
ATGACGGGTA ACGTGGAGCT TTTAGAGGAG GGTAAGAAGC GTCTGCAGAG AGACCTGGAG 
GCAGCGAATA CTCAGTTTGA GGAGAAGGCC GCAGCCTATG ATAAGCTTGA GAAGACCAAG 
AACAGACTGC AGCAGGAGCT TGAGGACACG CTGATGGATT TGGACAACCA GAGACAGCTG 
GTGTCCAACC TTGAGAAGAA ACAGAAGAAG TTTGATCAGA TGCTTGCTGA AGAGAAGAGC 
ATCTCTAGTA AGTATGCAGA TGAGCGAGAT CGCGCAGAGG CCGAGGCTAG AGAGAAGGAG 
ACCAAGGCTC TGTCTTTGGC CAGAGCTCTG GAGGAAGCTC AAGAAGCCCG CGAGGAGTTC 
GAGAGAGCCA ATAAAGCCCT TCGTGCTGAA ATGGAGGATC TAGTCAGCTC CAAAGACGAC 
GTGGGCAAAA ACGTGCATGA GCTTGAGAAG TCAAAACGTG GCTTGGAGGC TCAAGTCGAA 
GAGATGAAGA CACAGCTGGA AGAGCTGGAA GATGAGCTAC AGGCTGCGGA AGATGCCAAA 
CTGCGTCTGG AAGTGAACAT GCAGGCTCTG AAGGCTCAAT TTGAGCGAGA CCTCCAGGGC 
AGAGATGAGC AAGGAGAGGA AAAGAAGAGG CAGCTGGTCA AGCAGGTGCG TGAGCTGGAA 
ACCGAACTTG AAGATGAGCG CAAGCAAAGG ACTGCACTTG CTGCTTCCAA GAAGAAGCTG 
GAGGGAGACC TAAAGGACCT TGAGGGACAG ATTGAGACGT CTAACAAGGG ACGAGACGAG 
GCCATCAAGC AGCTCCGAAA GCTCCAGGCT CAAATGAAAG ACTTCCAAAG AGAGCTGGAT 
GATGCTCATG CTGCCAGGGA AGAAGTTTTA TCCAGTGCCA AGGAGAATGA GAGAAAGGCC 
AAGACTCTGG AGGCTGAACT TCTACAGCTG CAAGAGGACC TGGCCGCAGC TGAGAGGGCT 
AAAAAGCAAG TTGAAGCTGA GAGAGATGAA CTGGCTGATG AGTTGGCCAG CAATGCATCT 
GGAAAGTCTG CACTGTCTGA TGAGAAGAGA CGGCTTGAAG CCAAGATTCA GCAGCTGGAG 
GAGGAACTGG AGGAGGAGCA AGGCAACATG GAGATGCTCA ATGACAGGCT GAGAAAGAGC 
GCACAGCAGG TCGACCAGCT CACCAACGAG CTGCAGGCCG AGCGCACCAC ATCCCAAAAG 
AATGAGAGCG CCAGACAGCT GATGGAGAGG CAGAATAAAG AGCTGAAGGC TAAACTGCAG 
GAGATGGAGA ACCAAGTCAA ATCCAAATTC AAGTCCTCCA TCTCAGCTCT CGAAGCCAAA 
GTGGCACAAC TGGAGGAGCA GCTTGAGCAG GAGAGCAGAG ATAAGCAGAA CACAGCCAAA 
GCGGTTCGCC AGAAGGACAA GAAGCTGAAA GAGATGATGA CCCAAGTGGA GGATGAACGA 
AAACAAGCTG AGCAATATAA AGACCAGGCG GATAAGGCGA CCGCTCGAGT CAAGCAGCTG 
AAGAGGCAGT TGGAGGAGAG CGAGGAAGAG TCTCAGAGAA TCACTGCGGC CCGCAGGAAA 
CTGCAGCGAG AGCTGGACGA GGCCACCGAG ACTAACGATG CCATGAGCCG TGAGGTCAAT 
TCCCTCAAGA GCAAACTCAG AGGCAATCCT GAACCCACAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211981.1
CDS	119..5923
Translation	MTKKGLSDDEKFLFTDKDFINSPVAQADWSAKKLVWVPSEKHGFESASIKEEHGDEVLVELMDNGKKITVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYYSGLIYTYSGLFCVVVNPYKMLPIYSEKIIEMYKGKKRHEVPPHIYSITDNAYRNMMQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDMSAGELEKQLLQANPILEAFGNAKTIKNDNSSRFGKFIRINFDVTGFIVGANIETYLLEKSRCIRQAKTERAFHIFYYMVAGTKDKLREELLLENFNNYRFLSAGHVQIPGNQDDEMYDETMEAMEIMGFSVEERADVLKVVSTVLQLGNIEFKKERNQEQATMPDNTAAQKVCHLQGINVTDFTRAILTPRIKVGREVVQKAQTKEQADFAIEALAKAMYERLFRWILLRVNKALDKTKRQGASFLGILDIAGFEIFENNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGILALLDEECWFPKATDVSFVEKLCNTHANHTKFAKPKQLKDKTEFSVQHYAGRVDYNAVAWLTKNMDPLNDNVTALLNNSSNPFVQDLWKDADRVVGLETIAKMSDSLAPSASKTKKGMFRTVGQLYKESLAKLMTTLHNTQPNFVRCIIPNHEKRAGKLDAHLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACCLMIKHLDIDPNLYRIGQSKIFFRTGVLAQLEEERDLKITVIIIAFQSQARGFLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMSLKEEELQKAKESAQKFEIELKDIALKHTQLMDERNQLQEKLQAETELYAEAEEMRVRLASKKQELEEILHEMEARLEEEEDRGAALQMEKKKMHEQIKDLEEHLEEEEDARQKLQLEKVTCDAKIKKLEDDILIMDDQNNKLQKERKILEERIADFSSNLAEEEEKSKNLTKLKNKHESMISELEVRLKKEEKTRQELDKAKRKLEAESNDLQEQIADLQAQIADLKAQLAKKEEELQAALARLEDETGQKNNALKKIRELEGHISDLQEDLESERAARNKAEKTKRDLGEELEALKSELEDTLDTTATQQELRAKREQEVTLLKRAIEDESRVHEAQVQEMRQKHTQALEELTEQLEQSKRVKVNLEKAKQALEKETSELHVELRSLTQGKQDVEHKKKKLEGQLADLQSRFNDSERHKAELGDRVSKITVELESVTNLLNEAEGKNIKLSKDVASLSSQVQDTQELLAEETRQKLQLSTKLRQIEDDRNALQEQLDEEAEAKRNVERHVSTLNIQLSDFKKKLEEMTGNVELLEEGKKRLQRDLEAANTQFEEKAAAYDKLEKTKNRLQQELEDTLMDLDNQRQLVSNLEKKQKKFDQMLAEEKSISSKYADERDRAEAEAREKETKALSLARALEEAQEAREEFERANKALRAEMEDLVSSKDDVGKNVHELEKSKRGLEAQVEEMKTQLEELEDELQAAEDAKLRLEVNMQALKAQFERDLQGRDEQGEEKKRQLVKQVRELETELEDERKQRTALAASKKKLEGDLKDLEGQIETSNKGRDEAIKQLRKLQAQMKDFQRELDDAHAAREEVLSSAKENERKAKTLEAELLQLQEDLAAAERAKKQVEAERDELADELASNASGKSALSDEKRRLEAKIQQLEEELEEEQGNMEMLNDRLRKSAQQVDQLTNELQAERTTSQKNESARQLMERQNKELKAKLQEMENQVKSKFKSSISALEAKVAQLEEQLEQESRDKQNTAKAVRQKDKKLKEMMTQVEDERKQAEQYKDQADKATARVKQLKRQLEESEEESQRITAARRKLQRELDEATETNDAMSREVNSLKSKLRGNPEPTQ

Target ORF information:

RefSeq Version	XM_017356492.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356492.1

ORF Insert Sequence:

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTAATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCC GGCCTCATCT ACACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTGGT GAGCTGGAAA AGCAACTACT GCAGGCCAAT 
CCCATTCTAG AGGCCTTCGG TAATGCCAAG ACCATCAAGA ACGATAACTC TTCCCGATTC 
GGCAAATTTA TCCGCATTAA CTTTGATGTG ACAGGTTTCA TAGTTGGAGC CAACATCGAG 
ACCTATCTGT TGGAGAAATC TCGCTGTATC AGACAGGCGA AAACAGAAAG AGCTTTCCAC 
ATCTTTTACT ACATGGTGGC AGGAACAAAG GACAAACTAC GTGAGGAGCT TTTGCTGGAA 
AACTTCAACA ACTACCGTTT CCTCTCGGCT GGCCATGTCC AGATTCCAGG CAACCAGGAT 
GATGAGATGT ATGATGAAAC TATGGAGGCC ATGGAGATCA TGGGCTTCAG TGTTGAGGAA 
AGAGCAGATG TCCTCAAAGT AGTTTCAACT GTGCTGCAGT TGGGTAACAT TGAGTTCAAG 
AAAGAGAGGA ACCAAGAACA AGCTACCATG CCAGACAACA CAGCTGCACA GAAAGTTTGC 
CATCTTCAAG GCATCAATGT GACAGACTTC ACAAGAGCCA TTCTGACACC ACGTATTAAA 
GTGGGTCGTG AGGTTGTGCA AAAGGCCCAA ACCAAAGAAC AGGCTGATTT TGCCATTGAA 
GCTTTAGCTA AAGCTATGTA CGAACGCTTG TTCCGTTGGA TCCTTCTAAG AGTCAACAAA 
GCGTTAGACA AGACGAAACG CCAGGGTGCA TCTTTCCTGG GGATCTTGGA CATCGCTGGC 
TTTGAGATAT TTGAGAACAA TTCCTTCGAG CAGCTCTGCA TCAACTACAC CAATGAGAAG 
CTTCAGCAGC TCTTTAACCA CACCATGTTC ATCTTGGAGC AGGAGGAGTA TCAGCGGGAA 
GGCATCGAAT GGAACTTCAT CGACTTTGGG CTGGATTTAC AACCTTGCAT TGAGCTTATT 
GAAAGACCGA ACAATCCTCC AGGCATCTTG GCTTTGCTGG ATGAAGAATG TTGGTTTCCA 
AAGGCAACAG ACGTGTCCTT TGTGGAGAAA CTTTGCAACA CTCATGCTAA TCATACGAAG 
TTTGCAAAGC CTAAGCAACT GAAAGACAAG ACTGAATTCT CTGTGCAACA TTATGCTGGA 
CGGGTGGATT ACAATGCCGT AGCATGGTTG ACCAAAAACA TGGACCCTCT AAATGACAAC 
GTTACAGCAC TGCTGAATAA TTCCTCCAAT CCATTTGTGC AAGATCTGTG GAAAGATGCT 
GATCGTGTGG TTGGATTGGA AACCATTGCT AAGATGTCGG ACAGCCTTGC ACCTAGTGCC 
TCTAAGACCA AGAAGGGCAT GTTCCGTACT GTGGGCCAAC TTTACAAAGA GTCCCTGGCC 
AAGCTGATGA CCACACTGCA CAACACACAG CCAAACTTTG TCCGCTGTAT TATCCCCAAC 
CATGAGAAAC GGGCAGGGAA GCTGGATGCC CACCTGGTGC TAGAGCAGTT GAGATGTAAT 
GGTGTGCTAG AGGGCATTCG CATCTGCCGC CAAGGATTTC CAAACAGAAT TGTCTTCCAG 
GAGTTCAGAC AGCGATATGA GATCCTGGCA GCGAATGCTA TTCCAAAAGG CTTCATGGAT 
GGCAAGCAGG CCTGCTGTCT GATGATTAAG CACCTGGACA TTGATCCAAA CCTGTACCGA 
ATTGGTCAGA GCAAAATCTT CTTCCGCACT GGAGTCCTGG CTCAGCTTGA AGAGGAGCGA 
GACCTTAAGA TCACAGTCAT CATCATTGCC TTCCAGTCAC AGGCCAGGGG ATTCCTAGCC 
AGAAAGGCCT TTGCCAAGAG GCAGCAACAG CTAACAGCTA TGAAGGTGAT CCAGAGGAAC 
TGCGCTGCTT ATCTGAAACT CAGGAACTGG CAGTGGTGGA GACTCTTCAC TAAGGTAAAG 
CCTCTCCTGC AGGTCACGCG CCAAGAGGAA GAGATGAGCC TGAAAGAGGA GGAGCTCCAG 
AAGGCCAAAG AATCTGCACA GAAGTTTGAA ATCGAGCTAA AAGACATTGC ACTGAAGCAC 
ACACAGCTTA TGGATGAAAG GAACCAGCTC CAGGAGAAGC TGCAGGCAGA GACGGAGCTC 
TATGCGGAGG CAGAGGAGAT GAGAGTACGG TTGGCAAGCA AGAAACAGGA ACTGGAGGAG 
ATCTTACATG AGATGGAGGC CAGGTTGGAG GAAGAAGAGG ATCGCGGCGC AGCCCTGCAA 
ATGGAAAAGA AGAAAATGCA CGAACAGATT AAAGATCTTG AGGAGCATCT TGAGGAGGAA 
GAGGACGCTC GTCAGAAGCT GCAGCTGGAG AAGGTCACCT GTGATGCAAA GATCAAAAAG 
CTAGAGGATG ATATCCTGAT AATGGACGAC CAAAACAACA AACTGCAGAA GGAGAGGAAG 
ATTCTAGAAG AAAGGATAGC AGATTTTAGC TCAAACCTAG CAGAAGAAGA AGAAAAATCC 
AAAAACCTCA CAAAGCTGAA GAACAAGCAC GAGTCAATGA TCTCAGAACT GGAGGTGCGC 
TTAAAAAAAG AGGAGAAGAC ACGACAGGAG TTGGATAAAG CCAAGAGGAA GTTGGAGGCG 
GAATCCAATG ACCTCCAGGA GCAGATCGCT GACCTTCAAG CTCAGATTGC AGATCTGAAA 
GCCCAGCTGG CTAAGAAAGA GGAGGAGCTA CAGGCTGCAT TGGCGCGACT GGAGGATGAG 
ACAGGTCAGA AAAACAATGC TCTGAAGAAG ATCAGGGAGC TGGAGGGACA CATCTCGGAT 
CTTCAAGAAG ACCTGGAGTC AGAGCGGGCT GCGCGGAACA AGGCTGAAAA GACTAAAAGA 
GACTTGGGAG AGGAGCTTGA AGCTCTCAAA TCAGAGCTGG AGGACACACT GGACACCACA 
GCCACTCAGC AGGAGCTCAG GGCCAAACGT GAGCAGGAAG TGACTCTTTT GAAGAGAGCT 
ATAGAGGACG AGAGCCGCGT CCATGAGGCT CAGGTGCAGG AGATGAGACA GAAACACACC 
CAAGCTTTGG AGGAGCTCAC AGAGCAACTG GAGCAGTCCA AGAGGGTGAA GGTAAACCTG 
GAGAAAGCCA AGCAAGCTCT GGAGAAGGAG ACGTCAGAAC TCCACGTGGA GCTCCGTTCT 
CTTACTCAGG GCAAACAGGA TGTGGAGCAC AAGAAGAAGA AGCTGGAAGG ACAGCTGGCT 
GATCTGCAGT CTCGATTTAA TGACAGCGAG AGGCACAAAG CTGAGCTGGG AGATCGGGTC 
TCTAAAATCA CTGTGGAGCT GGAGAGCGTT ACCAACCTTC TCAATGAAGC TGAGGGAAAG 
AACATAAAGC TTAGCAAGGA TGTGGCTAGC TTGAGTTCTC AAGTTCAAGA CACACAGGAG 
TTGCTAGCCG AAGAAACAAG ACAGAAGCTG CAATTGTCTA CCAAGCTACG CCAAATAGAG 
GATGACCGCA ACGCCCTTCA GGAGCAGCTT GACGAGGAAG CAGAAGCCAA GAGAAATGTG 
GAGAGACATG TCTCAACACT TAACATTCAG CTCTCTGACT TCAAGAAGAA GCTGGAAGAA 
ATGACGGGTA ACGTGGAGCT TTTAGAGGAG GGTAAGAAGC GTCTGCAGAG AGACCTGGAG 
GCAGCGAATA CTCAGTTTGA GGAGAAGGCC GCAGCCTATG ATAAGCTTGA GAAGACCAAG 
AACAGACTGC AGCAGGAGCT TGAGGACACG CTGATGGATT TGGACAACCA GAGACAGCTG 
GTGTCCAACC TTGAGAAGAA ACAGAAGAAG TTTGATCAGA TGCTTGCTGA AGAGAAGAGC 
ATCTCTAGTA AGTATGCAGA TGAGCGAGAT CGCGCAGAGG CCGAGGCTAG AGAGAAGGAG 
ACCAAGGCTC TGTCTTTGGC CAGAGCTCTG GAGGAAGCTC AAGAAGCCCG CGAGGAGTTC 
GAGAGAGCCA ATAAAGCCCT TCGTGCTGAA ATGGAGGATC TAGTCAGCTC CAAAGACGAC 
GTGGGCAAAA ACGTGCATGA GCTTGAGAAG TCAAAACGTG GCTTGGAGGC TCAAGTCGAA 
GAGATGAAGA CACAGCTGGA AGAGCTGGAA GATGAGCTAC AGGCTGCGGA AGATGCCAAA 
CTGCGTCTGG AAGTGAACAT GCAGGCTCTG AAGGCTCAAT TTGAGCGAGA CCTCCAGGGC 
AGAGATGAGC AAGGAGAGGA AAAGAAGAGG CAGCTGGTCA AGCAGGTGCG TGAGCTGGAA 
ACCGAACTTG AAGATGAGCG CAAGCAAAGG ACTGCACTTG CTGCTTCCAA GAAGAAGCTG 
GAGGGAGACC TAAAGGACCT TGAGGGACAG ATTGAGACGT CTAACAAGGG ACGAGACGAG 
GCCATCAAGC AGCTCCGAAA GCTCCAGGCT CAAATGAAAG ACTTCCAAAG AGAGCTGGAT 
GATGCTCATG CTGCCAGGGA AGAAGTTTTA TCCAGTGCCA AGGAGAATGA GAGAAAGGCC 
AAGACTCTGG AGGCTGAACT TCTACAGCTG CAAGAGGACC TGGCCGCAGC TGAGAGGGCT 
AAAAAGCAAG TTGAAGCTGA GAGAGATGAA CTGGCTGATG AGTTGGCCAG CAATGCATCT 
GGAAAGTCTG CACTGTCTGA TGAGAAGAGA CGGCTTGAAG CCAAGATTCA GCAGCTGGAG 
GAGGAACTGG AGGAGGAGCA AGGCAACATG GAGATGCTCA ATGACAGGCT GAGAAAGAGC 
GCACAGCAGG TCGACCAGCT CACCAACGAG CTGCAGGCCG AGCGCACCAC ATCCCAAAAG 
AATGAGAGCG CCAGACAGCT GATGGAGAGG CAGAATAAAG AGCTGAAGGC TAAACTGCAG 
GAGATGGAGA ACCAAGTCAA ATCCAAATTC AAGTCCTCCA TCTCAGCTCT CGAAGCCAAA 
GTGGCACAAC TGGAGGAGCA GCTTGAGCAG GAGAGCAGAG ATAAGCAGAA CACAGCCAAA 
GCGGTTCGCC AGAAGGACAA GAAGCTGAAA GAGATGATGA CCCAAGTGGA GGATGAACGA 
AAACAAGCTG AGCAATATAA AGACCAGGCG GATAAGGCGA CCGCTCGAGT CAAGCAGCTG 
AAGAGGCAGT TGGAGGAGAG CGAGGAAGAG TCTCAGAGAA TCACTGCGGC CCGCAGGAAA 
CTGCAGCGAG AGCTGGACGA GGCCACCGAG ACTAACGATG CCATGAGCCG TGAGGTCAAT 
TCCCTCAAGA GCAAACTCAG AGGCAATCCT GAACCCACAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH11A ( NM_001024448.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001019619.1 Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), mRNA.

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CloneID	ODa03365
Clone ID Related Accession (Same CDS sequence)	NM_001024448.1
Accession Version	NM_001024448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	myosin-11
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ022376.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ022376.1, BC162152.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTGATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCT GGCCTCATCT ATACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTGGT GAGCTGGAAA AGCAACTACT GCAGGCCAAT 
CCCATTCTAG AGGCCTTCGG TAATGCCAAG ACCATCAAGA ACGATAACTC TTCCCGATTC 
GGCAAATTTA TCCGCATTAA CTTTGATGTG ACAGGTTTCA TAGTTGGAGC CAACATCGAG 
ACCTATCTGT TGGAGAAATC TCGCTGTATC AGACAGGCGA AAACAGAAAG AGCTTTCCAC 
ATCTTTTACT ACATGGTGGC AGGAACAAAG GACAAATTAC GTGAGGAGCT TTTGCTGGAA 
AACTTCAACA ACTACCGTTT CCTCTCGGCT GGCCATGTTC AGATTCCAGG CAACCAGGAT 
GATGAGATGT ATGATGAAAC TATGGAGGCC ATGGAGATCA TGGGCTTCAG TGTTGAGGAA 
AGAGCAGATG TCCTCAAAGT AGTTTCAACT GTGCTGCAGT TGGGTAACAT CGAGTTCAAG 
AAAGAAAGGA ACCAAGAACA AGCTACCATG CCAGACAACA CAGCTGCACA GAAAGTTTGC 
CATCTTCAAG GCATCAATGT GACAGACTTC ACAAGAGCCA TTCTGACACC ACGTATTAAA 
GCGGGTCGTG AGGTTGTGCA AAAGGCCCAA ACCAAAGAAC AGGCTGATTT TGCCATTGAA 
GCTTTAGCTA AAGCTATGTA CGAACGCTTG TTCCGTTGGA TCCTTCTAAG AGTCAACAAA 
GCGTTAGACA AGACGAAACG CCAGGGTGCA TCTTTCCTGG GGATCTTGGA CATCGCTGGC 
TTTGAGATAT TTGAGAACAA TTCCTTCGAG CAGCTCTGCA TCAACTACAC CAATGAGAAG 
CTTCAGCAGC TCTTTAACCA CACCATGTTC ATCTTGGAGC AGGAGGAGTA TCAGCGGGAA 
GGCATCGAAT GGAACTTCAT CGACTTTGGG CTGGATTTAC AACCTTGCAT TGAGCTTATT 
GAAAGACCGA ACAATCCTCC AGGCATCTTG GCTTTGCTGG ATGAAGAATG TTGGTTTCCA 
AAGGCAACAG ACGTGTCCTT TGTGGAGAAA CTTTGCAACA CTCATGCTAA TCATACGAAG 
TTTGCAAAAC CTAAGCAACT GAAAGACAAA ACTGAATTCT CTGTGCAACA TTATGCTGGA 
CGGGTGGATT ACAATGCCGT AGCATGGTTG ACCAAAAACA TGGACCCTCT AAATGACAAC 
GTTACAGCAC TGCTGAATAA TTCCTCCAAT CCGTTTGTGC AAGATCTGTG GAAAGATGCT 
GATCGTGTGG TTGGATTGGA AACCATTGCT AAGATGTCGG ACAGCCTTGC ACCTAGTGCC 
TCTAAGACCA AGAAGGGCAT GTTCCGTACT GTGGGCCAGC TTTACAAAGA GTCCCTGGCC 
AAGCTGATGA CCACACTGCA CAACACACAG CCAAACTTTG TCCGCTGTAT TATCCCCAAC 
CATGAGAAAC GGGCAGGGAA GCTGGATGCC CACCTGGTGC TAGAGCAGTT GAGATGTAAT 
GGTGTGCTAG AGGGCATTCG CATCTGCCGC CAAGGATTTC CAAACAGAAT TGTCTTCCAG 
GAGTTCCGAC AGCGATATGA GATCCTGGCA GCGAATGCTA TTCCAAAAGG CTTCATGGAT 
GGCAAGCAGG CCTGCTGTCT GATGATTAAG CACCTGGACA TTGATCCAAA CCTGTACCGA 
ATTGGTCAGA GCAAAATCTT CTTCCGCACT GGAGTCCTGG CTCAGCTTGA AGAGGAGCGA 
GACCTTAAGA TCACAGTCAT CATCATTGCC TTCCAGTCAC AGGCCAGGGG ATTCCTAGCC 
AGAAAGGCCT TTGCCAAGAG GCAGCAACAG CTAACAGCTA TGAAGGTGAT CCAGAGGAAC 
TGCGCTGCTT ATCTGAAACT CAGGAACTGG CAGTGGTGGA GACTCTTCAC TAAGGTAAAG 
CCTCTCCTGC AGGTCACGCG CCAAGAGGAG GAGATGAGCC TGAAAGAGGA GGAGCTCCAG 
AAGGCCAAAG AATCTGCACA GAAGTTTGAA ATCGAGCTAA AAGACATTGC ACTGAAGCAC 
ACACAGCTTA TGGATGAAAG GAACCAGCTC CAGGAGAAGC TGCAGGCAGA GACGGAGCTT 
TATGCGGAGG CAGAGGAGAT GAGAGTACGG TTGGCAAGCA AGAAACAGGA ACTGGAGGAG 
ATCTTACATG AGATGGAGGC CAGGTTGGAG GAAGAAGAGG ATCGCGGCGC AGCCCTGCAA 
ATGGAAAAGA AGAAAATGCA CGAACAGATT AAAGATCTTG AGGAGCATCT TGAGGAGGAA 
GAGGACGCTC GTCAGAAGCT GCAGCTGGAG AAGGTCACCT GTGATGCAAA GATCAAAAAG 
CTAGAGGATG ATATCCTGAT AATGGACGAC CAAAACAACA AACTGCAGAA GGAGAGGAAG 
ATTCTAGAAG AAAGGATAGC AGATTTTAGC TCAAACCTAG CGGAAGAAGA AGAAAAATCC 
AAAAACCTCA CAAAGCTGAA GAACAAGCAC GAGTCAATGA TCTCAGAACT GGAGGTGCGC 
TTGAAAAAAG AGGAGAAGAC ACGACAGGAG TTGGATAAAG CCAAGAGGAA GTTGGAGGCG 
GAATCCAATG ACCTCCAGGA GCAGATCGCT GACCTTCAAG CTCAGATTGC AGATCTGAAA 
GCCCAGCTGG CTAAGAAAGA GGAGGAGCTA CAGGCTGCAT TGGCGCGACT GGAGGATGAG 
ACAGGTCAGA AAAACAATGC TCTGAAGAAG ATCAGGGAGC TGGAGGGACA CATCTCGGAT 
CTTCAAGAAG ACCTGGAGTC AGAGCGGGCT GCGCGGAACA AGGCTGAAAA GACCAAAAGA 
GACTTGGGAG AGGAGCTTGA AGCTCTCAAA TCAGAGCTGG AGGACACACT GGACACCACA 
GCCACTCAGC AGGAGCTCAG GGCCAAACGT GAGCAGGAAG TGACTCTTTT GAAGAGAGCT 
ATAGAGGACG AGAGCCGCGT CCATGAGGCT CAGGTGCAGG AGATGAGACA GAAACACACC 
CAAGCTTTGG AGGAGCTCAC AGAGCAACTG GAGCAGTCCA AGAGGGTGAA GGTAAACCTG 
GAGAAAGCCA AGCAAGCTCT GGAGAAGGAG ACGTCAGAAC TCCACGTGGA GCTTCGTTCT 
CTTACTCAGG GCAAACAGGA TGTGGAGCAC AAGAAGAAGA AGCTGGAAGG ACAGCTGGCT 
GATCTGCAGT CTCGATTTAA TGACAGCGAG AGGCACAAAG CTGAGCTGGG AGATCGGGTC 
TCTAAAATCA CTGTGGAGCT GGAGAGCGTT ACCAACCTTC TCAATGAAGC TGAGGGAAAG 
AACATAAAGC TTAGCAAGGA TGTGGCTAGC TTGAGTTCTC AAGTTCAAGA CACACAGGAG 
TTGCTAGCTG AAGAAACAAG ACAGAAGCTG CAATTGTCTA CCAAGCTACG CCAAATAGAG 
GATGACCGCA ACGCCCTTCA GGAGCAGCTT GACGAGGAAG CAGAAGCCAA GAGAAATGTG 
GAGAGACATG TCTCAACACT TAACATTCAG CTCTCTGACT TCAAGAAGAA GCTGGAAGAA 
ATGACGGGTA ACGTGGAGCT TTTAGAGGAG GGTAAGAAGC GTCTGCAGAG AGACCTGGAG 
GCAGCGAATA CTCAGTTTGA GGAGAAGGCC GCAGCCTATG ATAAGCTTGA GAAGACCAAG 
AACAGACTGC AGCAGGAGCT TGAGGACACG CTGATGGATT TGGACAACCA GAGACAGCTG 
GTGTCCAACC TTGAGAAGAA ACAGAAGAAG TTTGATCAGA TGCTTGCTGA AGAGAAGAGC 
ATCTCTAGTA AGTATGCAGA TGAGCGAGAT CGCGCAGAGG CCGAGGCTAG AGAGAAGGAG 
ACCAAGGCTC TGTCTTTGGC CAGAGCTCTG GAGGAAGCTC AAGAAGCCCG CGAGGAGTTC 
GAGAGAGCCA ATAAAGCCCT TCGTGCTGAA ATGGAGGATC TAGTCAGCTC CAAAGACGAC 
GTGGGCAAAA ACGTGCATGA GCTTGAGAAG TCAAAACGTG GCTTGGAGGC TCAAGTCGAA 
GAGATGAAGA CACAGCTGGA AGAGCTGGAA GATGAGCTAC AGGCTGCGGA AGATGCCAAA 
CTGCGTCTGG AAGTGAACAT GCAGGCTCTG AAGGCTCAAT TTGAGCGAGA CCTCCAGGGC 
AGAGATGAGC AAGGAGAGGA AAAGAAGAGG CAGCTGGTCA AGCAGGTGCG TGAGCTGGAA 
ACCGAACTTG AAGATGAGCG CAAGCAAAGG ACTGCACTTG CTGCTTCCAA GAAGAAGCTG 
GAGGGAGACC TAAAGGACCT TGAGGGACAG ATTGAGACGT CTAACAAGGG ACGAGACGAG 
GCCATCAAGC AGCTCCGAAA GCTCCAGGCT CAAATGAAAG ACTTCCAAAG AGAGCTGGAT 
GATGCTCATG CTGCCAGGGA AGAAGTTTTA TCCAGTGCCA AGGAGAATGA GAGAAAGGCC 
AAGACTCTGG AGGCTGAACT TCTACAGCTG CAAGAGGACC TGGCCGCAGC TGAGAGGGCT 
AAAAAGCAAG TTGAAGCTGA GAGAGATGAA CTGGCTGATG AGTTGGCCAG CAATGCATCT 
GGAAAGTCTG CACTGTCTGA TGAGAAGAGA CGGCTTGAAG CCAAGATTCA GCAGCTGGAG 
GAGGAACTGG AGGAGGAGCA AGGCAACATG GAGATGCTCA ATGACAGGCT GAGAAAGAGC 
GCACAGCAGG TCGACCAGCT CACCAACGAG CTGCAGGCCG AGCGCACCAC ATCCCAAAAG 
AATGAGAGCG CCAGACAGCT GATGGAGAGG CAGAATAAAG AGCTGAAGGC TAAACTGCAG 
GAGATGGAGA ACCAAGTCAA ATCCAAATTC AAGTCCTCCA TCTCAGCTCT CGAAGCCAAA 
GTGGCACAAC TGGAGGAGCA GCTTGAGCAG GAGAGCAGAG ATAAGCAGAA CACAGCCAAA 
GCGGTTCGCC AGAAGGACAA GAAGCTGAAA GAGATGATGA CCCAAGTGGA GGATGAACGA 
AAACAAGCTG AGCAATATAA AGACCAGGCG GATAAGGCGA CCGCTCGAGT CAAGCAGCTG 
AAGAGGCAGT TGGAGGAGAG CGAGGAAGAG TCTCAGAGAA TCACTGCGGC CCGCAGGAAA 
CTGCAGCGAG AGCTGGACGA GGCCACCGAG ACTAACGATG CCATGAGCCG TGAGGTCAAT 
TCCCTCAAGA GCAAACTCAG GCGAGGCAAT GAGACGTCAT CCTTCAGCAG CACACCGAGA 
CGTACTGGAG GAGGAGGCCG ACGGGGAATC ATCGACAGCT CGGATGCTGC GGAGGATGAT 
GCCGACATGC AGAGCGATTA CAACGGAACC AAATCTAATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019619.1
CDS	113..6037
Translation	MTKKGLSDDEKFLFTDKDFINSPVAQADWSAKKLVWVPSEKHGFESASIKEEHGDEVLVELMDNGKKITVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYYSGLIYTYSGLFCVVVNPYKMLPIYSEKIIEMYKGKKRHEVPPHIYSITDNAYRNMMQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDMSAGELEKQLLQANPILEAFGNAKTIKNDNSSRFGKFIRINFDVTGFIVGANIETYLLEKSRCIRQAKTERAFHIFYYMVAGTKDKLREELLLENFNNYRFLSAGHVQIPGNQDDEMYDETMEAMEIMGFSVEERADVLKVVSTVLQLGNIEFKKERNQEQATMPDNTAAQKVCHLQGINVTDFTRAILTPRIKAGREVVQKAQTKEQADFAIEALAKAMYERLFRWILLRVNKALDKTKRQGASFLGILDIAGFEIFENNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGILALLDEECWFPKATDVSFVEKLCNTHANHTKFAKPKQLKDKTEFSVQHYAGRVDYNAVAWLTKNMDPLNDNVTALLNNSSNPFVQDLWKDADRVVGLETIAKMSDSLAPSASKTKKGMFRTVGQLYKESLAKLMTTLHNTQPNFVRCIIPNHEKRAGKLDAHLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACCLMIKHLDIDPNLYRIGQSKIFFRTGVLAQLEEERDLKITVIIIAFQSQARGFLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMSLKEEELQKAKESAQKFEIELKDIALKHTQLMDERNQLQEKLQAETELYAEAEEMRVRLASKKQELEEILHEMEARLEEEEDRGAALQMEKKKMHEQIKDLEEHLEEEEDARQKLQLEKVTCDAKIKKLEDDILIMDDQNNKLQKERKILEERIADFSSNLAEEEEKSKNLTKLKNKHESMISELEVRLKKEEKTRQELDKAKRKLEAESNDLQEQIADLQAQIADLKAQLAKKEEELQAALARLEDETGQKNNALKKIRELEGHISDLQEDLESERAARNKAEKTKRDLGEELEALKSELEDTLDTTATQQELRAKREQEVTLLKRAIEDESRVHEAQVQEMRQKHTQALEELTEQLEQSKRVKVNLEKAKQALEKETSELHVELRSLTQGKQDVEHKKKKLEGQLADLQSRFNDSERHKAELGDRVSKITVELESVTNLLNEAEGKNIKLSKDVASLSSQVQDTQELLAEETRQKLQLSTKLRQIEDDRNALQEQLDEEAEAKRNVERHVSTLNIQLSDFKKKLEEMTGNVELLEEGKKRLQRDLEAANTQFEEKAAAYDKLEKTKNRLQQELEDTLMDLDNQRQLVSNLEKKQKKFDQMLAEEKSISSKYADERDRAEAEAREKETKALSLARALEEAQEAREEFERANKALRAEMEDLVSSKDDVGKNVHELEKSKRGLEAQVEEMKTQLEELEDELQAAEDAKLRLEVNMQALKAQFERDLQGRDEQGEEKKRQLVKQVRELETELEDERKQRTALAASKKKLEGDLKDLEGQIETSNKGRDEAIKQLRKLQAQMKDFQRELDDAHAAREEVLSSAKENERKAKTLEAELLQLQEDLAAAERAKKQVEAERDELADELASNASGKSALSDEKRRLEAKIQQLEEELEEEQGNMEMLNDRLRKSAQQVDQLTNELQAERTTSQKNESARQLMERQNKELKAKLQEMENQVKSKFKSSISALEAKVAQLEEQLEQESRDKQNTAKAVRQKDKKLKEMMTQVEDERKQAEQYKDQADKATARVKQLKRQLEESEEESQRITAARRKLQRELDEATETNDAMSREVNSLKSKLRRGNETSSFSSTPRRTGGGGRRGIIDSSDAAEDDADMQSDYNGTKSNE

Target ORF information:

RefSeq Version	NM_001024448.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy chain 11a, smooth muscle (myh11a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024448.1

ORF Insert Sequence:

ATGACGAAGA AAGGCTTGAG CGATGATGAG AAATTCCTTT TCACGGACAA AGACTTCATC 
AACAGCCCCG TGGCCCAGGC CGATTGGTCA GCCAAAAAGC TGGTGTGGGT CCCCTCCGAA 
AAGCACGGCT TCGAGTCTGC CAGCATCAAA GAGGAACATG GAGACGAGGT GCTGGTGGAG 
CTGATGGATA ATGGAAAGAA GATCACCGTC AACAAGGACG ACATCCAGAA GATGAACCCG 
CCCAAGTTCA GCAAGGTGGA GGACATGGCG GAGCTCACCT GCCTGAATGA AGCCTCCGTC 
CTGCACAACC TGAGGGAACG CTACTACTCT GGCCTCATCT ATACATACTC TGGGCTGTTT 
TGCGTGGTGG TGAACCCATA TAAAATGCTG CCAATCTACT CTGAGAAGAT CATCGAAATG 
TATAAAGGCA AAAAGCGCCA TGAGGTCCCT CCTCACATCT ATTCCATCAC GGACAACGCC 
TACAGGAACA TGATGCAAGA TCGAGAGGAC CAGTCTATTC TTTGCACTGG TGAATCAGGG 
GCTGGAAAGA CAGAAAACAC CAAGAAAGTC ATCCAGTATT TGGCTGTAGT GGCCTCATCC 
CACAAAGGCA AGAAAGACAT GAGCGCTGGT GAGCTGGAAA AGCAACTACT GCAGGCCAAT 
CCCATTCTAG AGGCCTTCGG TAATGCCAAG ACCATCAAGA ACGATAACTC TTCCCGATTC 
GGCAAATTTA TCCGCATTAA CTTTGATGTG ACAGGTTTCA TAGTTGGAGC CAACATCGAG 
ACCTATCTGT TGGAGAAATC TCGCTGTATC AGACAGGCGA AAACAGAAAG AGCTTTCCAC 
ATCTTTTACT ACATGGTGGC AGGAACAAAG GACAAATTAC GTGAGGAGCT TTTGCTGGAA 
AACTTCAACA ACTACCGTTT CCTCTCGGCT GGCCATGTTC AGATTCCAGG CAACCAGGAT 
GATGAGATGT ATGATGAAAC TATGGAGGCC ATGGAGATCA TGGGCTTCAG TGTTGAGGAA 
AGAGCAGATG TCCTCAAAGT AGTTTCAACT GTGCTGCAGT TGGGTAACAT CGAGTTCAAG 
AAAGAAAGGA ACCAAGAACA AGCTACCATG CCAGACAACA CAGCTGCACA GAAAGTTTGC 
CATCTTCAAG GCATCAATGT GACAGACTTC ACAAGAGCCA TTCTGACACC ACGTATTAAA 
GCGGGTCGTG AGGTTGTGCA AAAGGCCCAA ACCAAAGAAC AGGCTGATTT TGCCATTGAA 
GCTTTAGCTA AAGCTATGTA CGAACGCTTG TTCCGTTGGA TCCTTCTAAG AGTCAACAAA 
GCGTTAGACA AGACGAAACG CCAGGGTGCA TCTTTCCTGG GGATCTTGGA CATCGCTGGC 
TTTGAGATAT TTGAGAACAA TTCCTTCGAG CAGCTCTGCA TCAACTACAC CAATGAGAAG 
CTTCAGCAGC TCTTTAACCA CACCATGTTC ATCTTGGAGC AGGAGGAGTA TCAGCGGGAA 
GGCATCGAAT GGAACTTCAT CGACTTTGGG CTGGATTTAC AACCTTGCAT TGAGCTTATT 
GAAAGACCGA ACAATCCTCC AGGCATCTTG GCTTTGCTGG ATGAAGAATG TTGGTTTCCA 
AAGGCAACAG ACGTGTCCTT TGTGGAGAAA CTTTGCAACA CTCATGCTAA TCATACGAAG 
TTTGCAAAAC CTAAGCAACT GAAAGACAAA ACTGAATTCT CTGTGCAACA TTATGCTGGA 
CGGGTGGATT ACAATGCCGT AGCATGGTTG ACCAAAAACA TGGACCCTCT AAATGACAAC 
GTTACAGCAC TGCTGAATAA TTCCTCCAAT CCGTTTGTGC AAGATCTGTG GAAAGATGCT 
GATCGTGTGG TTGGATTGGA AACCATTGCT AAGATGTCGG ACAGCCTTGC ACCTAGTGCC 
TCTAAGACCA AGAAGGGCAT GTTCCGTACT GTGGGCCAGC TTTACAAAGA GTCCCTGGCC 
AAGCTGATGA CCACACTGCA CAACACACAG CCAAACTTTG TCCGCTGTAT TATCCCCAAC 
CATGAGAAAC GGGCAGGGAA GCTGGATGCC CACCTGGTGC TAGAGCAGTT GAGATGTAAT 
GGTGTGCTAG AGGGCATTCG CATCTGCCGC CAAGGATTTC CAAACAGAAT TGTCTTCCAG 
GAGTTCCGAC AGCGATATGA GATCCTGGCA GCGAATGCTA TTCCAAAAGG CTTCATGGAT 
GGCAAGCAGG CCTGCTGTCT GATGATTAAG CACCTGGACA TTGATCCAAA CCTGTACCGA 
ATTGGTCAGA GCAAAATCTT CTTCCGCACT GGAGTCCTGG CTCAGCTTGA AGAGGAGCGA 
GACCTTAAGA TCACAGTCAT CATCATTGCC TTCCAGTCAC AGGCCAGGGG ATTCCTAGCC 
AGAAAGGCCT TTGCCAAGAG GCAGCAACAG CTAACAGCTA TGAAGGTGAT CCAGAGGAAC 
TGCGCTGCTT ATCTGAAACT CAGGAACTGG CAGTGGTGGA GACTCTTCAC TAAGGTAAAG 
CCTCTCCTGC AGGTCACGCG CCAAGAGGAG GAGATGAGCC TGAAAGAGGA GGAGCTCCAG 
AAGGCCAAAG AATCTGCACA GAAGTTTGAA ATCGAGCTAA AAGACATTGC ACTGAAGCAC 
ACACAGCTTA TGGATGAAAG GAACCAGCTC CAGGAGAAGC TGCAGGCAGA GACGGAGCTT 
TATGCGGAGG CAGAGGAGAT GAGAGTACGG TTGGCAAGCA AGAAACAGGA ACTGGAGGAG 
ATCTTACATG AGATGGAGGC CAGGTTGGAG GAAGAAGAGG ATCGCGGCGC AGCCCTGCAA 
ATGGAAAAGA AGAAAATGCA CGAACAGATT AAAGATCTTG AGGAGCATCT TGAGGAGGAA 
GAGGACGCTC GTCAGAAGCT GCAGCTGGAG AAGGTCACCT GTGATGCAAA GATCAAAAAG 
CTAGAGGATG ATATCCTGAT AATGGACGAC CAAAACAACA AACTGCAGAA GGAGAGGAAG 
ATTCTAGAAG AAAGGATAGC AGATTTTAGC TCAAACCTAG CGGAAGAAGA AGAAAAATCC 
AAAAACCTCA CAAAGCTGAA GAACAAGCAC GAGTCAATGA TCTCAGAACT GGAGGTGCGC 
TTGAAAAAAG AGGAGAAGAC ACGACAGGAG TTGGATAAAG CCAAGAGGAA GTTGGAGGCG 
GAATCCAATG ACCTCCAGGA GCAGATCGCT GACCTTCAAG CTCAGATTGC AGATCTGAAA 
GCCCAGCTGG CTAAGAAAGA GGAGGAGCTA CAGGCTGCAT TGGCGCGACT GGAGGATGAG 
ACAGGTCAGA AAAACAATGC TCTGAAGAAG ATCAGGGAGC TGGAGGGACA CATCTCGGAT 
CTTCAAGAAG ACCTGGAGTC AGAGCGGGCT GCGCGGAACA AGGCTGAAAA GACCAAAAGA 
GACTTGGGAG AGGAGCTTGA AGCTCTCAAA TCAGAGCTGG AGGACACACT GGACACCACA 
GCCACTCAGC AGGAGCTCAG GGCCAAACGT GAGCAGGAAG TGACTCTTTT GAAGAGAGCT 
ATAGAGGACG AGAGCCGCGT CCATGAGGCT CAGGTGCAGG AGATGAGACA GAAACACACC 
CAAGCTTTGG AGGAGCTCAC AGAGCAACTG GAGCAGTCCA AGAGGGTGAA GGTAAACCTG 
GAGAAAGCCA AGCAAGCTCT GGAGAAGGAG ACGTCAGAAC TCCACGTGGA GCTTCGTTCT 
CTTACTCAGG GCAAACAGGA TGTGGAGCAC AAGAAGAAGA AGCTGGAAGG ACAGCTGGCT 
GATCTGCAGT CTCGATTTAA TGACAGCGAG AGGCACAAAG CTGAGCTGGG AGATCGGGTC 
TCTAAAATCA CTGTGGAGCT GGAGAGCGTT ACCAACCTTC TCAATGAAGC TGAGGGAAAG 
AACATAAAGC TTAGCAAGGA TGTGGCTAGC TTGAGTTCTC AAGTTCAAGA CACACAGGAG 
TTGCTAGCTG AAGAAACAAG ACAGAAGCTG CAATTGTCTA CCAAGCTACG CCAAATAGAG 
GATGACCGCA ACGCCCTTCA GGAGCAGCTT GACGAGGAAG CAGAAGCCAA GAGAAATGTG 
GAGAGACATG TCTCAACACT TAACATTCAG CTCTCTGACT TCAAGAAGAA GCTGGAAGAA 
ATGACGGGTA ACGTGGAGCT TTTAGAGGAG GGTAAGAAGC GTCTGCAGAG AGACCTGGAG 
GCAGCGAATA CTCAGTTTGA GGAGAAGGCC GCAGCCTATG ATAAGCTTGA GAAGACCAAG 
AACAGACTGC AGCAGGAGCT TGAGGACACG CTGATGGATT TGGACAACCA GAGACAGCTG 
GTGTCCAACC TTGAGAAGAA ACAGAAGAAG TTTGATCAGA TGCTTGCTGA AGAGAAGAGC 
ATCTCTAGTA AGTATGCAGA TGAGCGAGAT CGCGCAGAGG CCGAGGCTAG AGAGAAGGAG 
ACCAAGGCTC TGTCTTTGGC CAGAGCTCTG GAGGAAGCTC AAGAAGCCCG CGAGGAGTTC 
GAGAGAGCCA ATAAAGCCCT TCGTGCTGAA ATGGAGGATC TAGTCAGCTC CAAAGACGAC 
GTGGGCAAAA ACGTGCATGA GCTTGAGAAG TCAAAACGTG GCTTGGAGGC TCAAGTCGAA 
GAGATGAAGA CACAGCTGGA AGAGCTGGAA GATGAGCTAC AGGCTGCGGA AGATGCCAAA 
CTGCGTCTGG AAGTGAACAT GCAGGCTCTG AAGGCTCAAT TTGAGCGAGA CCTCCAGGGC 
AGAGATGAGC AAGGAGAGGA AAAGAAGAGG CAGCTGGTCA AGCAGGTGCG TGAGCTGGAA 
ACCGAACTTG AAGATGAGCG CAAGCAAAGG ACTGCACTTG CTGCTTCCAA GAAGAAGCTG 
GAGGGAGACC TAAAGGACCT TGAGGGACAG ATTGAGACGT CTAACAAGGG ACGAGACGAG 
GCCATCAAGC AGCTCCGAAA GCTCCAGGCT CAAATGAAAG ACTTCCAAAG AGAGCTGGAT 
GATGCTCATG CTGCCAGGGA AGAAGTTTTA TCCAGTGCCA AGGAGAATGA GAGAAAGGCC 
AAGACTCTGG AGGCTGAACT TCTACAGCTG CAAGAGGACC TGGCCGCAGC TGAGAGGGCT 
AAAAAGCAAG TTGAAGCTGA GAGAGATGAA CTGGCTGATG AGTTGGCCAG CAATGCATCT 
GGAAAGTCTG CACTGTCTGA TGAGAAGAGA CGGCTTGAAG CCAAGATTCA GCAGCTGGAG 
GAGGAACTGG AGGAGGAGCA AGGCAACATG GAGATGCTCA ATGACAGGCT GAGAAAGAGC 
GCACAGCAGG TCGACCAGCT CACCAACGAG CTGCAGGCCG AGCGCACCAC ATCCCAAAAG 
AATGAGAGCG CCAGACAGCT GATGGAGAGG CAGAATAAAG AGCTGAAGGC TAAACTGCAG 
GAGATGGAGA ACCAAGTCAA ATCCAAATTC AAGTCCTCCA TCTCAGCTCT CGAAGCCAAA 
GTGGCACAAC TGGAGGAGCA GCTTGAGCAG GAGAGCAGAG ATAAGCAGAA CACAGCCAAA 
GCGGTTCGCC AGAAGGACAA GAAGCTGAAA GAGATGATGA CCCAAGTGGA GGATGAACGA 
AAACAAGCTG AGCAATATAA AGACCAGGCG GATAAGGCGA CCGCTCGAGT CAAGCAGCTG 
AAGAGGCAGT TGGAGGAGAG CGAGGAAGAG TCTCAGAGAA TCACTGCGGC CCGCAGGAAA 
CTGCAGCGAG AGCTGGACGA GGCCACCGAG ACTAACGATG CCATGAGCCG TGAGGTCAAT 
TCCCTCAAGA GCAAACTCAG GCGAGGCAAT GAGACGTCAT CCTTCAGCAG CACACCGAGA 
CGTACTGGAG GAGGAGGCCG ACGGGGAATC ATCGACAGCT CGGATGCTGC GGAGGATGAT 
GCCGACATGC AGAGCGATTA CAACGGAACC AAATCTAATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution mRNA expression time course of embryonic development in zebrafish.
eLife6(2017 Nov)
White RJ,Collins JE,Sealy IM,Wali N,Dooley CM,Digby Z,Stemple DL,Murphy DN,Billis K,Hourlier T,F?llgrabe A,Davis MP,Enright AJ,Busch-Nentwich EM

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

An exclusive cellular and molecular network governs intestinal smooth muscle cell differentiation in vertebrates.
Development (Cambridge, England)144(3)464-478(2017 02)
Gays D,Hess C,Camporeale A,Ala U,Provero P,Mosimann C,Santoro MM

Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
Science advances2(11)e1600844(2016 Nov)
Chen WC,Wang Z,Missinato MA,Park DW,Long DW,Liu HJ,Zeng X,Yates NA,Kim K,Wang Y

Graded effects of unregulated smooth muscle myosin on intestinal architecture, intestinal motility and vascular function in zebrafish.
Disease models & mechanisms9(5)529-40(2016 05)
Abrams J,Einhorn Z,Seiler C,Zong AB,Sweeney HL,Pack M

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MYH11A cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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