zap70 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zap70
Entrez Gene ID	553615
Full Name	zeta chain of T cell receptor associated protein kinase 70
Synonyms	zgc:110383
General protein information	Preferred Names zeta chain of T cell receptor associated protein kinase 70 Names tyrosine-protein kinase ZAP-70 zeta-chain (TCR) associated protein kinase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020589.1	NP_001018425.1	tyrosine-protein kinase ZAP-70

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZAP70	NP_001070.2
Canis lupus familiaris (dog)	ZAP70	XP_005626103.1
Gallus gallus (chicken)	ZAP70	XP_418206.4
Xenopus tropicalis (tropical clawed frog)	zap70	NP_001006825.1
	ZAP70	XP_001101570.2
Pan troglodytes (chimpanzee)	ZAP70	XP_515637.2
Mus musculus (house mouse)	Zap70	NP_033565.2
Rattus norvegicus (Norway rat)	Zap70	NP_001012002.1
Danio rerio (zebrafish)	zap70	NP_001018425.1
Bos taurus (cattle)	ZAP70	NP_001179946.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Tang Q, Iyer S, Lobbardi R, Moore JC, Chen H, Lareau C, Hebert C, Shaw ML, Neftel C, Suva ML, Ceol CJ, Bernards A, Aryee M, Pinello L, Drummond IA, Langenau DM
The Journal of experimental medicine214(10)2875-2887(2017 Oct)

T cell immune deficiency in zap70 mutant zebrafish.
Moore JC, Mulligan TS, Torres Yord?n N, Castranova D, Pham VN, Tang Q, Lobbardi R, Anselmo A, Liwski RS, Berman JN, Sadreyev RI, Weinstein BM, Langenau DM
Molecular and cellular biology(2016 Sep)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio).
Yoon S, Mitra S, Wyse C, Alnabulsi A, Zou J, Weerdenburg EM, van der Sar AM, Wang D, Secombes CJ, Bird S
PloS one10(6)e0126378(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

syk and zap-70 function redundantly in an early progenitor to promote the migration of intersegmental vessel angioblasts and lymphangioblasts that contribute to the thoracic duct, either downstream of, or in parallel to vegfa.
Title: Syk and Zap-70 function redundantly to promote angioblast migration.

The following zap70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zap70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34331	NM_001020589.1 Latest version!	Danio rerio zeta chain of T cell receptor associated protein kinase 70 (zap70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zap70 ( NM_001020589.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018425.1 Danio rerio zeta chain of T cell receptor associated protein kinase 70 (zap70), mRNA.

Price & Availability↑

CloneID	ODa34331
Clone ID Related Accession (Same CDS sequence)	NM_001020589.1
Accession Version	NM_001020589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein kinase ZAP-70
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095237.1. ##Evidence-Data-START## Transcript exon combination :: BC095237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCGAAC CTGCCGCCGA TCTCCCATTC TTCTATGGCA GCATCAGTCG CTCGGAGGCC 
GAGGAGCACC TCAAGCTGGC TGGTATGGGA GGCGGCCTGT TCCTCCTGCG ACAGTGCCTG 
CGCAGTCTGG GTGGCTATGT GCTGTCCATG ATCTGGAACC TGGACTTCTA CCATTACTCC 
ATTGAGAAAC AGCTCAACGG AACGTATTGT ATCGCAGGAG GGAAGCCTCA CTGTGGACCA 
GCGGAGCTCT GTGAATACTA CAGCAAAGAC CCCGACGGCC TTGTGTGCAC CTTGAGGAAG 
CCCTGTCTGA GATCGCCTGA AACAGAAATC CGGAAAGGCG TCTTCGATAA CCTGAGGGAC 
AACATGCTCA GGGAATATGT GCGGCATACC TGGAAACTGG AGGGGGATGC CATGGAGCAA 
GCCATCATCA GCCAGGCTCC ACAGCTAGAG AAACTGATCG CCACCACAGC GCATGAGAAA 
ATGCCCTGGT TCCACGGCAA GATCCCTCGG CAGGAGGGGG AAAGACGACT GTACTCTGGA 
TCTCAGCCGG ACGGAAAGTT CCTAGTTCGA GAGAGAGATG AAATGGGCAC CTTTGCACTG 
TCTGTGACGT ATGGAAAAAC TGTGTACCAT TATCAGATCT TGCGTGACAA ATCAGGGAAA 
ATCGCAATGC CAGAAGGAAC TAAATTTGAC ACTGTGTGGC AGCTGGTTGA ATACTTAAAA 
ATGAAGCCTG ATGGTCTTGT GACTGTATTG AGAGAACCGT GTGTTAACCA AAACAACGCA 
GCTCCAGCTC CTGCAAACGC TCCGAGGAGG TCAAGAGGTA ATGGATACAC ACCGCCCCCT 
ATGGTGCCTA AGCCAATGGG AGCTGAGGGT TCACGCCCAT CTCTACCAAT GGACCATGAC 
GGATTTACTA GTCCTTATGA CGATCCAAAC GAACTGAAGA AAAAGACCCT GTTCATCAAA 
AGAGACAAAT TGATGATCGA CGAGGTGGAG CTTGGGTCTG GAAACTTCGG CTGCGTCAAG 
AAAGGTGTTT TCAAAATGGA AAGCAAACAG ATCGATGTGG CAATTAAAGT GCTGAAGAAT 
GAGAACGAGA AATCGGTGAG GGATGAAATG ATGCGAGAGG CTGAGATTAT GCATCAGCTG 
AGTGATCCCT TCATTGTGCG CATGATTGGA CTCTGTGAGG CCGAAGCGCT CATGCTGGTG 
ATGGAAATGG CCCCTGCCGG CCCGCTCAAC AAGTTCCTCT CTGGAAAGAA AGACCAGATC 
ACTACGGAAA ACATTGTGAT GTTAATGCAT CAGGTGTCAA TGGGGATGAA GTATTTAGAA 
GGAAGGAACT TTGTGCACAG AGATCTGGCT GCTCGTAATG TGCTCCTGGT CAACCAGCAA 
TATGCCAAAA TCAGCGACTT CGGCCTCTCC AAAGCACTGG GTGCAGATGA TAACTATTAC 
AAGGCCAGAA CAGGAGGAAA ATGGCCTCTG AAGTGGTACG CGCCAGAGTG CATTCATTTT 
CACAAGTTTT CCAGCAAAAG TGACGTTTGG AGTTTTGGCA TCACGATGTG GGAGGCCTTT 
TCATTTGGAG GAAAGCCGTA TAAGAAAATG AAAGGTCCAG AGGTGATAAC ATATATTGAA 
GGTGGGAGTC GTTTGGATTG TCCTGCCGCA TGTCCTGAGG CCATGTATGA GCTGATGAAA 
GAATGCTGGA CCTACAAGCA TGAAGAACGG CTAAATTTTG CAAAAGTGGA GGAGAAGATG 
AGAACTTTTT ATTACTCCAT AGCAAAGAAG ATCCCGGATT ACCTGAAAAC AGAGAATGGA 
ACTCCTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018425.1
CDS	150..1964
Translation	MTEPAADLPFFYGSISRSEAEEHLKLAGMGGGLFLLRQCLRSLGGYVLSMIWNLDFYHYSIEKQLNGTYCIAGGKPHCGPAELCEYYSKDPDGLVCTLRKPCLRSPETEIRKGVFDNLRDNMLREYVRHTWKLEGDAMEQAIISQAPQLEKLIATTAHEKMPWFHGKIPRQEGERRLYSGSQPDGKFLVRERDEMGTFALSVTYGKTVYHYQILRDKSGKIAMPEGTKFDTVWQLVEYLKMKPDGLVTVLREPCVNQNNAAPAPANAPRRSRGNGYTPPPMVPKPMGAEGSRPSLPMDHDGFTSPYDDPNELKKKTLFIKRDKLMIDEVELGSGNFGCVKKGVFKMESKQIDVAIKVLKNENEKSVRDEMMREAEIMHQLSDPFIVRMIGLCEAEALMLVMEMAPAGPLNKFLSGKKDQITTENIVMLMHQVSMGMKYLEGRNFVHRDLAARNVLLVNQQYAKISDFGLSKALGADDNYYKARTGGKWPLKWYAPECIHFHKFSSKSDVWSFGITMWEAFSFGGKPYKKMKGPEVITYIEGGSRLDCPAACPEAMYELMKECWTYKHEERLNFAKVEEKMRTFYYSIAKKIPDYLKTENGTPPK

Target ORF information:

RefSeq Version	NM_001020589.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zeta chain of T cell receptor associated protein kinase 70 (zap70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020589.1

ORF Insert Sequence:

ATGACCGAAC CTGCCGCCGA TCTCCCATTC TTCTATGGCA GCATCAGTCG CTCGGAGGCC 
GAGGAGCACC TCAAGCTGGC TGGTATGGGA GGCGGCCTGT TCCTCCTGCG ACAGTGCCTG 
CGCAGTCTGG GTGGCTATGT GCTGTCCATG ATCTGGAACC TGGACTTCTA CCATTACTCC 
ATTGAGAAAC AGCTCAACGG AACGTATTGT ATCGCAGGAG GGAAGCCTCA CTGTGGACCA 
GCGGAGCTCT GTGAATACTA CAGCAAAGAC CCCGACGGCC TTGTGTGCAC CTTGAGGAAG 
CCCTGTCTGA GATCGCCTGA AACAGAAATC CGGAAAGGCG TCTTCGATAA CCTGAGGGAC 
AACATGCTCA GGGAATATGT GCGGCATACC TGGAAACTGG AGGGGGATGC CATGGAGCAA 
GCCATCATCA GCCAGGCTCC ACAGCTAGAG AAACTGATCG CCACCACAGC GCATGAGAAA 
ATGCCCTGGT TCCACGGCAA GATCCCTCGG CAGGAGGGGG AAAGACGACT GTACTCTGGA 
TCTCAGCCGG ACGGAAAGTT CCTAGTTCGA GAGAGAGATG AAATGGGCAC CTTTGCACTG 
TCTGTGACGT ATGGAAAAAC TGTGTACCAT TATCAGATCT TGCGTGACAA ATCAGGGAAA 
ATCGCAATGC CAGAAGGAAC TAAATTTGAC ACTGTGTGGC AGCTGGTTGA ATACTTAAAA 
ATGAAGCCTG ATGGTCTTGT GACTGTATTG AGAGAACCGT GTGTTAACCA AAACAACGCA 
GCTCCAGCTC CTGCAAACGC TCCGAGGAGG TCAAGAGGTA ATGGATACAC ACCGCCCCCT 
ATGGTGCCTA AGCCAATGGG AGCTGAGGGT TCACGCCCAT CTCTACCAAT GGACCATGAC 
GGATTTACTA GTCCTTATGA CGATCCAAAC GAACTGAAGA AAAAGACCCT GTTCATCAAA 
AGAGACAAAT TGATGATCGA CGAGGTGGAG CTTGGGTCTG GAAACTTCGG CTGCGTCAAG 
AAAGGTGTTT TCAAAATGGA AAGCAAACAG ATCGATGTGG CAATTAAAGT GCTGAAGAAT 
GAGAACGAGA AATCGGTGAG GGATGAAATG ATGCGAGAGG CTGAGATTAT GCATCAGCTG 
AGTGATCCCT TCATTGTGCG CATGATTGGA CTCTGTGAGG CCGAAGCGCT CATGCTGGTG 
ATGGAAATGG CCCCTGCCGG CCCGCTCAAC AAGTTCCTCT CTGGAAAGAA AGACCAGATC 
ACTACGGAAA ACATTGTGAT GTTAATGCAT CAGGTGTCAA TGGGGATGAA GTATTTAGAA 
GGAAGGAACT TTGTGCACAG AGATCTGGCT GCTCGTAATG TGCTCCTGGT CAACCAGCAA 
TATGCCAAAA TCAGCGACTT CGGCCTCTCC AAAGCACTGG GTGCAGATGA TAACTATTAC 
AAGGCCAGAA CAGGAGGAAA ATGGCCTCTG AAGTGGTACG CGCCAGAGTG CATTCATTTT 
CACAAGTTTT CCAGCAAAAG TGACGTTTGG AGTTTTGGCA TCACGATGTG GGAGGCCTTT 
TCATTTGGAG GAAAGCCGTA TAAGAAAATG AAAGGTCCAG AGGTGATAAC ATATATTGAA 
GGTGGGAGTC GTTTGGATTG TCCTGCCGCA TGTCCTGAGG CCATGTATGA GCTGATGAAA 
GAATGCTGGA CCTACAAGCA TGAAGAACGG CTAAATTTTG CAAAAGTGGA GGAGAAGATG 
AGAACTTTTT ATTACTCCAT AGCAAAGAAG ATCCCGGATT ACCTGAAAAC AGAGAATGGA 
ACTCCTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
The Journal of experimental medicine214(10)2875-2887(2017 Oct)
Tang Q,Iyer S,Lobbardi R,Moore JC,Chen H,Lareau C,Hebert C,Shaw ML,Neftel C,Suva ML,Ceol CJ,Bernards A,Aryee M,Pinello L,Drummond IA,Langenau DM

T cell immune deficiency in zap70 mutant zebrafish.
Molecular and cellular biology(2016 Sep)
Moore JC,Mulligan TS,Torres Yord?n N,Castranova D,Pham VN,Tang Q,Lobbardi R,Anselmo A,Liwski RS,Berman JN,Sadreyev RI,Weinstein BM,Langenau DM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio).
PloS one10(6)e0126378(2015)
Yoon S,Mitra S,Wyse C,Alnabulsi A,Zou J,Weerdenburg EM,van der Sar AM,Wang D,Secombes CJ,Bird S

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zap70 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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