Zap70 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zap70
Entrez Gene ID	22637
Full Name	zeta-chain (TCR) associated protein kinase
Synonyms	Srk,ZAP-70,mrtle,mur
General protein information	Preferred Names zeta-chain (TCR) associated protein kinase Names tyrosine-protein kinase ZAP-70 70 kDa zeta-chain associated protein syk-related tyrosine kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 B\|1 15.41 cM
Summary	This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006495896.3	XP_006495959.1	tyrosine-protein kinase ZAP-70 isoform X1
NM_001289612.1	NP_001276541.1	tyrosine-protein kinase ZAP-70 isoform TZK
NM_009539.3	NP_033565.2	tyrosine-protein kinase ZAP-70 isoform a
NM_001289765.1	NP_001276694.1	tyrosine-protein kinase ZAP-70 isoform a
XM_006495896.2	XP_006495959.1	tyrosine-protein kinase ZAP-70 isoform X1
NM_001289766.1	NP_001276695.1	tyrosine-protein kinase ZAP-70 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZAP70	NP_001070.2
Canis lupus familiaris (dog)	ZAP70	XP_005626103.1
Gallus gallus (chicken)	ZAP70	XP_418206.4
Xenopus tropicalis (tropical clawed frog)	zap70	NP_001006825.1
	ZAP70	XP_001101570.2
Pan troglodytes (chimpanzee)	ZAP70	XP_515637.2
Mus musculus (house mouse)	Zap70	NP_033565.2
Rattus norvegicus (Norway rat)	Zap70	NP_001012002.1
Danio rerio (zebrafish)	zap70	NP_001018425.1
Bos taurus (cattle)	ZAP70	NP_001179946.1

Gene Ontology
Bibliography

Related articles in PubMed

??TCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program.
Muro R, Nitta T, Nakano K, Okamura T, Takayanagi H, Suzuki H
The Journal of clinical investigation128(1)415-426(2018 Jan)

Identification of a novel isoform of ZAP-70, truncated ZAP kinase.
Kuroyama H, Ikeda T, Kasai M, Yamasaki S, Tatsumi M, Utsuyama M, Saito T, Hirokawa K
Biochemical and biophysical research communications315(4)935-41(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

disruption of normal Zap70 autoinhibition engaged negative feedback mechanisms by which negative selection and inhibitory receptors restrain TCR signaling to enforce both central and peripheral tolerance.
Title: Destabilizing the autoinhibitory conformation of Zap70 induces up-regulation of inhibitory receptors and T cell unresponsiveness.

in this study, authors discovered a cycle of recruitment, activation and release for Zap70 kinases at phosphorylated T-cell antigen receptors, which turned them into a 'catalytic unit' that amplified antigenic stimuli
Title: A cycle of Zap70 kinase activation and release from the TCR amplifies and disperses antigenic stimuli.

this study shows that ZAP-70 mutation has a strong impact on T cell-driven arthritis in SKG model regardless of the genetic background
Title: The SKG Mutation in ZAP-70 also Confers Arthritis Susceptibility in C57 Black Mouse Strains.

These findings establish Otud7b as a positive regulator of T cell receptor-proximal signaling and T cell activation, highlighting the importance of deubiquitination in regulating Zap70 function.
Title: Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.

was significantly increased in 22-month AEW mice compared with 5-month AEW mice. ELISA data showed that secretions of IL-2 and NGF in 22-month AEW mice were higher than 5-month AEW mice. Our results indicate that increased ZAP70 is involved in dry skin in elderly pruritus. Increased secretion of IL-2 and NGF may induce dry skin itch.
Title: Increased ZAP70 Is Involved in Dry Skin Pruritus in Aged Mice.

The following Zap70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zap70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19829	NM_009539.3 Latest version!	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19829	NM_001289765.1 Latest version!	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19865	NM_001289766.1 Latest version!	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19889	NM_001289612.1 Latest version!	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu19829	XM_006495896.3 Latest version!	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu19829	XM_006495896.2	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zap70 ( NM_009539.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033565.2 Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19829
Clone ID Related Accession (Same CDS sequence)	XM_006495896.3 , NM_009539.3 , NM_001289765.1 , XM_006495896.2
Accession Version	NM_009539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ZAP-70 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084389.1, AK039883.1 and BY589032.1. On Jan 25, 2014 this sequence version replaced NM_009539.2. Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 4. The encoded isoform (a) has a shorter N-terminus compared to isoform b. Variants 1 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK089056.1, BC029727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033565.2
CDS	155..2011
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAAVAAPTLPAHPSTFTQPQRRVDTLNSDGYTPEPARLASSTDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	NM_009539.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009539.3

ORF Insert Sequence:

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zap70 ( NM_001289765.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276694.1 Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu19829
Clone ID Related Accession (Same CDS sequence)	XM_006495896.3 , NM_009539.3 , NM_001289765.1 , XM_006495896.2
Accession Version	NM_001289765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ZAP-70 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084389.1, CJ143180.1, AK039883.1 and BY589032.1. On Jan 31, 2014 this sequence version replaced XM_006495897.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 4. The encoded isoform (a) has a shorter N-terminus compared to isoform b. Variants 1 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC029727.1, U77667.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276694.1
CDS	198..2054
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAAVAAPTLPAHPSTFTQPQRRVDTLNSDGYTPEPARLASSTDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	NM_001289765.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289765.1

ORF Insert Sequence:

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zap70 ( NM_001289766.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276695.1 Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 4, mRNA.

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CloneID	OMu19865
Clone ID Related Accession (Same CDS sequence)	NM_001289766.1
Accession Version	NM_001289766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ZAP-70 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084389.1. On Jan 31, 2014 this sequence version replaced XM_006495895.1. Transcript Variant: This variant (4) encodes the longest isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAAATCT GTCTTGGAAA ACCAGGAAGG AGCCTGTTAC CCTCTGTGGT GTGCTTACTA 
CAGGGTCCAG CGATGCCCGA TCCCGCGGCG CACCTGCCAT TCTTCTATGG CAGCATCTCG 
CGGGCTGAGG CCGAGGAGCA CCTGAAGCTG GCAGGCATGG CCGACGGGCT GTTCCTCCTG 
CGCCAGTGTT TGCGCTCCCT GGGCGGCTAC GTGCTGTCGT TGGTGCACGA CGTGCGCTTC 
CACCATTTCC CCATCGAGCG CCAACTCAAC GGCACGTACG CCATCGCGGG CGGGAAGGCG 
CACTGCGGCC CGGCCGAGCT CTGCCAGTTC TACTCTCAGG ACCCCGACGG GCTGCCCTGC 
AACCTGCGTA AGCCGTGTAA CCGGCCGCCC GGACTGGAGC CACAGCCCGG GGTCTTCGAC 
TGCCTGCGTG ATGCTATGGT GCGCGACTAC GTGCGCCAGA CCTGGAAGCT GGAGGGCGAT 
GCACTAGAGC AGGCCATCAT CAGCCAGGCC CCACAGGTGG AGAAGCTCAT TGCTACCACA 
GCCCACGAGC GAATGCCCTG GTATCACAGC AGCCTGACTC GTGAGGAGGC CGAGCGCAAA 
CTCTATTCCG GCCAGCAGAC CGACGGCAAG TTCCTGCTGA GGCCCCGGAA GGAGCAGGGC 
ACATATGCAC TGTCCCTGGT CTATGGGAAA ACTGTATACC ACTATCTCAT CAGCCAGGAC 
AAGGCTGGCA AGTACTGCAT CCCCGAAGGC ACCAAGTTTG ACACGCTCTG GCAGCTGGTG 
GAGTACCTGA AGCTGAAGGC GGATGGACTA ATTTACCGCT TGAAGGAGGT CTGTCCCAAC 
AGCAGCGCCA GCGCGGCAGT TGCTGCACCC ACCCTCCCAG CCCACCCATC CACATTCACT 
CAGCCTCAGA GACGAGTGGA CACCCTGAAC TCGGACGGAT ACACCCCTGA ACCAGCACGC 
TTAGCGTCAT CGACAGACAA GCCCCGGCCG ATGCCCATGG ACACAAGTGT GTACGAGAGT 
CCCTACAGCG ACCCTGAGGA ACTCAAAGAC AAGAAGCTCT TCCTGAAGCG AGAGAATCTC 
CTCGTGGCGG ACATCGAGCT TGGCTGTGGC AACTTTGGCT CCGTGCGCCA GGGAGTCTAT 
CGCATGCGCA AGAAGCAGAT TGACGTGGCC ATCAAGGTGC TGAAGCAGGG CACAGAGAAG 
GCCGACAAAG ATGAGATGAT GCGAGAGGCC CAGATCATGC ACCAGCTCGA CAACCCCTAC 
ATCGTGCGGC TCATCGGCGT GTGCCAGGCA GAAGCACTCA TGCTGGTCAT GGAGATGGCG 
GGAGGCGGGC CCCTGCACAA GTTCCTGCTG GGAAAGAAGG AGGAGATCCC TGTGAGCAAT 
GTGGCTGAAC TGCTGCACCA GGTGGCCATG GGCATGAAGT ATTTGGAGGA GAAAAACTTT 
GTGCACCGCG ACCTGGCAGC CCGCAATGTT CTACTGGTCA ATCGGCACTA TGCCAAGATC 
AGCGACTTTG GCCTGTCCAA AGCCCTGGGT GCTGACGACA GCTATTACAC AGCCCGGTCT 
GCAGGGAAGT GGCCTCTGAA GTGGTACGCG CCAGAGTGCA TCAACTTTCG GAAGTTCTCC 
AGCCGCAGTG ACGTCTGGAG CTATGGGGTC ACCATGTGGG AGGCCTTCTC CTATGGCCAG 
AAGCCCTACA AGAAAATGAA GGGCCCCGAG GTCCTGGACT TCATCAAGCA GGGTAAGAGG 
ATGGAATGTC CGCCGGAGTG TCCTCCTGAG ATGTATGCAC TTATGAGTGA CTGCTGGATC 
TACAAGTGGG AGGATCGCCC CGACTTCCTG ACTGTGGAAC AACGTATGCG GAACTATTAC 
TACAGCCTGG CCAGCCGGGC CGAGGGACCC CCACAGTGTG AACAGGTGGC CGAGGCTGCA 
TGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276695.1
CDS	91..2019
Translation	MQICLGKPGRSLLPSVVCLLQGPAMPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAAVAAPTLPAHPSTFTQPQRRVDTLNSDGYTPEPARLASSTDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	NM_001289766.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289766.1

ORF Insert Sequence:

ATGCAAATCT GTCTTGGAAA ACCAGGAAGG AGCCTGTTAC CCTCTGTGGT GTGCTTACTA 
CAGGGTCCAG CGATGCCCGA TCCCGCGGCG CACCTGCCAT TCTTCTATGG CAGCATCTCG 
CGGGCTGAGG CCGAGGAGCA CCTGAAGCTG GCAGGCATGG CCGACGGGCT GTTCCTCCTG 
CGCCAGTGTT TGCGCTCCCT GGGCGGCTAC GTGCTGTCGT TGGTGCACGA CGTGCGCTTC 
CACCATTTCC CCATCGAGCG CCAACTCAAC GGCACGTACG CCATCGCGGG CGGGAAGGCG 
CACTGCGGCC CGGCCGAGCT CTGCCAGTTC TACTCTCAGG ACCCCGACGG GCTGCCCTGC 
AACCTGCGTA AGCCGTGTAA CCGGCCGCCC GGACTGGAGC CACAGCCCGG GGTCTTCGAC 
TGCCTGCGTG ATGCTATGGT GCGCGACTAC GTGCGCCAGA CCTGGAAGCT GGAGGGCGAT 
GCACTAGAGC AGGCCATCAT CAGCCAGGCC CCACAGGTGG AGAAGCTCAT TGCTACCACA 
GCCCACGAGC GAATGCCCTG GTATCACAGC AGCCTGACTC GTGAGGAGGC CGAGCGCAAA 
CTCTATTCCG GCCAGCAGAC CGACGGCAAG TTCCTGCTGA GGCCCCGGAA GGAGCAGGGC 
ACATATGCAC TGTCCCTGGT CTATGGGAAA ACTGTATACC ACTATCTCAT CAGCCAGGAC 
AAGGCTGGCA AGTACTGCAT CCCCGAAGGC ACCAAGTTTG ACACGCTCTG GCAGCTGGTG 
GAGTACCTGA AGCTGAAGGC GGATGGACTA ATTTACCGCT TGAAGGAGGT CTGTCCCAAC 
AGCAGCGCCA GCGCGGCAGT TGCTGCACCC ACCCTCCCAG CCCACCCATC CACATTCACT 
CAGCCTCAGA GACGAGTGGA CACCCTGAAC TCGGACGGAT ACACCCCTGA ACCAGCACGC 
TTAGCGTCAT CGACAGACAA GCCCCGGCCG ATGCCCATGG ACACAAGTGT GTACGAGAGT 
CCCTACAGCG ACCCTGAGGA ACTCAAAGAC AAGAAGCTCT TCCTGAAGCG AGAGAATCTC 
CTCGTGGCGG ACATCGAGCT TGGCTGTGGC AACTTTGGCT CCGTGCGCCA GGGAGTCTAT 
CGCATGCGCA AGAAGCAGAT TGACGTGGCC ATCAAGGTGC TGAAGCAGGG CACAGAGAAG 
GCCGACAAAG ATGAGATGAT GCGAGAGGCC CAGATCATGC ACCAGCTCGA CAACCCCTAC 
ATCGTGCGGC TCATCGGCGT GTGCCAGGCA GAAGCACTCA TGCTGGTCAT GGAGATGGCG 
GGAGGCGGGC CCCTGCACAA GTTCCTGCTG GGAAAGAAGG AGGAGATCCC TGTGAGCAAT 
GTGGCTGAAC TGCTGCACCA GGTGGCCATG GGCATGAAGT ATTTGGAGGA GAAAAACTTT 
GTGCACCGCG ACCTGGCAGC CCGCAATGTT CTACTGGTCA ATCGGCACTA TGCCAAGATC 
AGCGACTTTG GCCTGTCCAA AGCCCTGGGT GCTGACGACA GCTATTACAC AGCCCGGTCT 
GCAGGGAAGT GGCCTCTGAA GTGGTACGCG CCAGAGTGCA TCAACTTTCG GAAGTTCTCC 
AGCCGCAGTG ACGTCTGGAG CTATGGGGTC ACCATGTGGG AGGCCTTCTC CTATGGCCAG 
AAGCCCTACA AGAAAATGAA GGGCCCCGAG GTCCTGGACT TCATCAAGCA GGGTAAGAGG 
ATGGAATGTC CGCCGGAGTG TCCTCCTGAG ATGTATGCAC TTATGAGTGA CTGCTGGATC 
TACAAGTGGG AGGATCGCCC CGACTTCCTG ACTGTGGAAC AACGTATGCG GAACTATTAC 
TACAGCCTGG CCAGCCGGGC CGAGGGACCC CCACAGTGTG AACAGGTGGC CGAGGCTGCA 
TGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zap70 ( XM_006495896.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495959.1 Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant X1, mRNA.

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CloneID	OMu19829
Clone ID Related Accession (Same CDS sequence)	XM_006495896.3 , NM_009539.3 , NM_001289765.1 , XM_006495896.2
Accession Version	XM_006495896.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ZAP-70 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495959.1
CDS	203..2059
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAAVAAPTLPAHPSTFTQPQRRVDTLNSDGYTPEPARLASSTDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	XM_006495896.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495896.2

ORF Insert Sequence:

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zap70 ( NM_001289612.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276541.1 Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 2, mRNA.

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CloneID	OMu19889
Clone ID Related Accession (Same CDS sequence)	NM_001289612.1
Accession Version	NM_001289612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ZAP-70 isoform TZK
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084389.1. Transcript Variant: This variant (2) contains a 5' terminal exon that extends past a splice site that is used in variant 1. This variant differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 4. The encoded isoform (TZK, also known as truncated ZAP kinase; PMID 14985102) has a shorter N-terminus compared to isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB083210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATGG ACACAAGTGT GTACGAGAGT CCCTACAGCG ACCCTGAGGA ACTCAAAGAC 
AAGAAGCTCT TCCTGAAGCG AGAGAATCTC CTCGTGGCGG ACATCGAGCT TGGCTGTGGC 
AACTTTGGCT CCGTGCGCCA GGGAGTCTAT CGCATGCGCA AGAAGCAGAT TGACGTGGCC 
ATCAAGGTGC TGAAGCAGGG CACAGAGAAG GCCGACAAAG ATGAGATGAT GCGAGAGGCC 
CAGATCATGC ACCAGCTCGA CAACCCCTAC ATCGTGCGGC TCATCGGCGT GTGCCAGGCA 
GAAGCACTCA TGCTGGTCAT GGAGATGGCG GGAGGCGGGC CCCTGCACAA GTTCCTGCTG 
GGAAAGAAGG AGGAGATCCC TGTGAGCAAT GTGGCTGAAC TGCTGCACCA GGTGGCCATG 
GGCATGAAGT ATTTGGAGGA GAAAAACTTT GTGCACCGCG ACCTGGCAGC CCGCAATGTT 
CTACTGGTCA ATCGGCACTA TGCCAAGATC AGCGACTTTG GCCTGTCCAA AGCCCTGGGT 
GCTGACGACA GCTATTACAC AGCCCGGTCT GCAGGGAAGT GGCCTCTGAA GTGGTACGCG 
CCAGAGTGCA TCAACTTTCG GAAGTTCTCC AGCCGCAGTG ACGTCTGGAG CTATGGGGTC 
ACCATGTGGG AGGCCTTCTC CTATGGCCAG AAGCCCTACA AGAAAATGAA GGGCCCCGAG 
GTCCTGGACT TCATCAAGCA GGGTAAGAGG ATGGAATGTC CGCCGGAGTG TCCTCCTGAG 
ATGTATGCAC TTATGAGTGA CTGCTGGATC TACAAGTGGG AGGATCGCCC CGACTTCCTG 
ACTGTGGAAC AACGTATGCG GAACTATTAC TACAGCCTGG CCAGCCGGGC CGAGGGACCC 
CCACAGTGTG AACAGGTGGC CGAGGCTGCA TGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276541.1
CDS	169..1107
Translation	MPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	NM_001289612.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289612.1

ORF Insert Sequence:

ATGCCCATGG ACACAAGTGT GTACGAGAGT CCCTACAGCG ACCCTGAGGA ACTCAAAGAC 
AAGAAGCTCT TCCTGAAGCG AGAGAATCTC CTCGTGGCGG ACATCGAGCT TGGCTGTGGC 
AACTTTGGCT CCGTGCGCCA GGGAGTCTAT CGCATGCGCA AGAAGCAGAT TGACGTGGCC 
ATCAAGGTGC TGAAGCAGGG CACAGAGAAG GCCGACAAAG ATGAGATGAT GCGAGAGGCC 
CAGATCATGC ACCAGCTCGA CAACCCCTAC ATCGTGCGGC TCATCGGCGT GTGCCAGGCA 
GAAGCACTCA TGCTGGTCAT GGAGATGGCG GGAGGCGGGC CCCTGCACAA GTTCCTGCTG 
GGAAAGAAGG AGGAGATCCC TGTGAGCAAT GTGGCTGAAC TGCTGCACCA GGTGGCCATG 
GGCATGAAGT ATTTGGAGGA GAAAAACTTT GTGCACCGCG ACCTGGCAGC CCGCAATGTT 
CTACTGGTCA ATCGGCACTA TGCCAAGATC AGCGACTTTG GCCTGTCCAA AGCCCTGGGT 
GCTGACGACA GCTATTACAC AGCCCGGTCT GCAGGGAAGT GGCCTCTGAA GTGGTACGCG 
CCAGAGTGCA TCAACTTTCG GAAGTTCTCC AGCCGCAGTG ACGTCTGGAG CTATGGGGTC 
ACCATGTGGG AGGCCTTCTC CTATGGCCAG AAGCCCTACA AGAAAATGAA GGGCCCCGAG 
GTCCTGGACT TCATCAAGCA GGGTAAGAGG ATGGAATGTC CGCCGGAGTG TCCTCCTGAG 
ATGTATGCAC TTATGAGTGA CTGCTGGATC TACAAGTGGG AGGATCGCCC CGACTTCCTG 
ACTGTGGAAC AACGTATGCG GAACTATTAC TACAGCCTGG CCAGCCGGGC CGAGGGACCC 
CCACAGTGTG AACAGGTGGC CGAGGCTGCA TGTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zap70 ( XM_006495896.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495959.1 Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19829
Clone ID Related Accession (Same CDS sequence)	XM_006495896.3 , NM_009539.3 , NM_001289765.1 , XM_006495896.2
Accession Version	XM_006495896.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ZAP-70 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495896.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495959.1
CDS	274..2130
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCQFYSQDPDGLPCNLRKPCNRPPGLEPQPGVFDCLRDAMVRDYVRQTWKLEGDALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGQQTDGKFLLRPRKEQGTYALSLVYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYRLKEVCPNSSASAAVAAPTLPAHPSTFTQPQRRVDTLNSDGYTPEPARLASSTDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRENLLVADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLLGKKEEIPVSNVAELLHQVAMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVLDFIKQGKRMECPPECPPEMYALMSDCWIYKWEDRPDFLTVEQRMRNYYYSLASRAEGPPQCEQVAEAACG

Target ORF information:

RefSeq Version	XM_006495896.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zeta-chain (TCR) associated protein kinase (Zap70), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495896.3

ORF Insert Sequence:

ATGCCCGATC CCGCGGCGCA CCTGCCATTC TTCTATGGCA GCATCTCGCG GGCTGAGGCC 
GAGGAGCACC TGAAGCTGGC AGGCATGGCC GACGGGCTGT TCCTCCTGCG CCAGTGTTTG 
CGCTCCCTGG GCGGCTACGT GCTGTCGTTG GTGCACGACG TGCGCTTCCA CCATTTCCCC 
ATCGAGCGCC AACTCAACGG CACGTACGCC ATCGCGGGCG GGAAGGCGCA CTGCGGCCCG 
GCCGAGCTCT GCCAGTTCTA CTCTCAGGAC CCCGACGGGC TGCCCTGCAA CCTGCGTAAG 
CCGTGTAACC GGCCGCCCGG ACTGGAGCCA CAGCCCGGGG TCTTCGACTG CCTGCGTGAT 
GCTATGGTGC GCGACTACGT GCGCCAGACC TGGAAGCTGG AGGGCGATGC ACTAGAGCAG 
GCCATCATCA GCCAGGCCCC ACAGGTGGAG AAGCTCATTG CTACCACAGC CCACGAGCGA 
ATGCCCTGGT ATCACAGCAG CCTGACTCGT GAGGAGGCCG AGCGCAAACT CTATTCCGGC 
CAGCAGACCG ACGGCAAGTT CCTGCTGAGG CCCCGGAAGG AGCAGGGCAC ATATGCACTG 
TCCCTGGTCT ATGGGAAAAC TGTATACCAC TATCTCATCA GCCAGGACAA GGCTGGCAAG 
TACTGCATCC CCGAAGGCAC CAAGTTTGAC ACGCTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCGG ATGGACTAAT TTACCGCTTG AAGGAGGTCT GTCCCAACAG CAGCGCCAGC 
GCGGCAGTTG CTGCACCCAC CCTCCCAGCC CACCCATCCA CATTCACTCA GCCTCAGAGA 
CGAGTGGACA CCCTGAACTC GGACGGATAC ACCCCTGAAC CAGCACGCTT AGCGTCATCG 
ACAGACAAGC CCCGGCCGAT GCCCATGGAC ACAAGTGTGT ACGAGAGTCC CTACAGCGAC 
CCTGAGGAAC TCAAAGACAA GAAGCTCTTC CTGAAGCGAG AGAATCTCCT CGTGGCGGAC 
ATCGAGCTTG GCTGTGGCAA CTTTGGCTCC GTGCGCCAGG GAGTCTATCG CATGCGCAAG 
AAGCAGATTG ACGTGGCCAT CAAGGTGCTG AAGCAGGGCA CAGAGAAGGC CGACAAAGAT 
GAGATGATGC GAGAGGCCCA GATCATGCAC CAGCTCGACA ACCCCTACAT CGTGCGGCTC 
ATCGGCGTGT GCCAGGCAGA AGCACTCATG CTGGTCATGG AGATGGCGGG AGGCGGGCCC 
CTGCACAAGT TCCTGCTGGG AAAGAAGGAG GAGATCCCTG TGAGCAATGT GGCTGAACTG 
CTGCACCAGG TGGCCATGGG CATGAAGTAT TTGGAGGAGA AAAACTTTGT GCACCGCGAC 
CTGGCAGCCC GCAATGTTCT ACTGGTCAAT CGGCACTATG CCAAGATCAG CGACTTTGGC 
CTGTCCAAAG CCCTGGGTGC TGACGACAGC TATTACACAG CCCGGTCTGC AGGGAAGTGG 
CCTCTGAAGT GGTACGCGCC AGAGTGCATC AACTTTCGGA AGTTCTCCAG CCGCAGTGAC 
GTCTGGAGCT ATGGGGTCAC CATGTGGGAG GCCTTCTCCT ATGGCCAGAA GCCCTACAAG 
AAAATGAAGG GCCCCGAGGT CCTGGACTTC ATCAAGCAGG GTAAGAGGAT GGAATGTCCG 
CCGGAGTGTC CTCCTGAGAT GTATGCACTT ATGAGTGACT GCTGGATCTA CAAGTGGGAG 
GATCGCCCCG ACTTCCTGAC TGTGGAACAA CGTATGCGGA ACTATTACTA CAGCCTGGCC 
AGCCGGGCCG AGGGACCCCC ACAGTGTGAA CAGGTGGCCG AGGCTGCATG TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

??TCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program.
The Journal of clinical investigation128(1)415-426(2018 Jan)
Muro R,Nitta T,Nakano K,Okamura T,Takayanagi H,Suzuki H

Identification of a novel isoform of ZAP-70, truncated ZAP kinase.
Biochemical and biophysical research communications315(4)935-41(2004 Mar)
Kuroyama H,Ikeda T,Kasai M,Yamasaki S,Tatsumi M,Utsuyama M,Saito T,Hirokawa K

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Zap70 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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