si:ch211-266g18.10 cDNA ORF clone, Danio rerio(zebrafish)

The following si:ch211-266g18.10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-266g18.10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149668 XM_021467332.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149669 XM_021467333.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149670 XM_021467334.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149671 XM_021467335.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149672 XM_021467336.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149673 XM_021467337.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149674 XM_021467338.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149675 XM_021467339.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149676 XM_021467340.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149677 XM_021467341.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149678 XM_021467342.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149679 XM_021467343.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149680 XM_021467344.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149681 XM_021467345.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149682 XM_021467346.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149683 XM_021467347.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149684 XM_021467348.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149685 XM_021467349.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149686 XM_021467350.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149687 XM_021467351.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149688 XM_021467352.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149689 XM_021467353.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149690 XM_021467354.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149691 XM_021467355.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149692 XM_021467356.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149693 XM_021467357.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149694 XM_021467358.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149695 XM_021467359.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149696 XM_021467360.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149697 XM_021467361.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149698 XM_021467362.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149699 XM_021467363.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149700 XM_021467364.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149701 XM_021467365.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149702 XM_021467366.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149703 XM_021467367.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149704 XM_021467368.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149705 XM_021467369.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149706 XM_021467370.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X39, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149707 XM_021467371.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149708 XM_021467372.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149709 XM_021467373.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149710 XM_021467374.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149711 XM_021467375.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X44, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149712 XM_021467376.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X45, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149713 XM_021467377.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X46, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149714 XM_021467378.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X47, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149715 XM_021467379.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X48, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149716 XM_021467380.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X49, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa149717 XM_021467381.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X50, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa55696 XM_017351778.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55697 XM_017351779.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55698 XM_017351780.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55699 XM_017351781.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55700 XM_017351782.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55701 XM_017351783.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55702 XM_017351784.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55703 XM_017351785.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa55704 XM_017351786.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa55705 XM_017351787.1
Latest version!
Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149668
    Clone ID Related Accession (Same CDS sequence) XM_021467332.1
    Accession Version XM_021467332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGAAGTC
    ATCAAAGAAA AAGTCAAACT TGCTCCTGTA AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG
    GCACTTAAAC CAGAACCCAA AAAAGATGAA GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC
    CTAAACTCCA TCAACCAAAA ACTGTCTCTC CTGGACTTGC TAAGGAAGGA CATTGGAGAA
    ATGAAAAGCG ATCTGGAGTC CACTCAGAAT CAAATCACAC TTTTACGGAT GGACAACAGA
    ACGATCAAAG AGCCAGAGAC TATTGGTGTG AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA
    AAAAGAGACC ACGCTCTGAT CAATGCTACT AGGACTGCAC ATATTAAAAC AGAGCGTGAA
    TCTCTAAAAA ACATCACGAA ACCAGTGCCT GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT
    AAGATTGCGC TGGAGCCTAA AAAGAAAGGA ATCATCCCTG AGAAGGTCAA AGAAGTAAAG
    GAAACTTTAC AGAAAGAACC TGAGTTGGTC AAAGATAAAG TAGTTGAGCC AATAGAGAAG
    GAAATTGTTG TTGAGAAGGA AGTCAAAGGA GAAGAGCCTG AAATCACCAA AGATATTCCA
    GAGGTGGAGG AAGAGGACAT TCCCTACTTC CAGTGTTTCT TTGTGGATGA GGATGACACT
    CAGTATCCGT TCTTCCCTTT CTCTCCACCG CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323007.1
    CDS338..8416
    Translation

    Target ORF information:

    RefSeq Version XM_021467332.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGAAGTC
    ATCAAAGAAA AAGTCAAACT TGCTCCTGTA AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG
    GCACTTAAAC CAGAACCCAA AAAAGATGAA GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC
    CTAAACTCCA TCAACCAAAA ACTGTCTCTC CTGGACTTGC TAAGGAAGGA CATTGGAGAA
    ATGAAAAGCG ATCTGGAGTC CACTCAGAAT CAAATCACAC TTTTACGGAT GGACAACAGA
    ACGATCAAAG AGCCAGAGAC TATTGGTGTG AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA
    AAAAGAGACC ACGCTCTGAT CAATGCTACT AGGACTGCAC ATATTAAAAC AGAGCGTGAA
    TCTCTAAAAA ACATCACGAA ACCAGTGCCT GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT
    AAGATTGCGC TGGAGCCTAA AAAGAAAGGA ATCATCCCTG AGAAGGTCAA AGAAGTAAAG
    GAAACTTTAC AGAAAGAACC TGAGTTGGTC AAAGATAAAG TAGTTGAGCC AATAGAGAAG
    GAAATTGTTG TTGAGAAGGA AGTCAAAGGA GAAGAGCCTG AAATCACCAA AGATATTCCA
    GAGGTGGAGG AAGAGGACAT TCCCTACTTC CAGTGTTTCT TTGTGGATGA GGATGACACT
    CAGTATCCGT TCTTCCCTTT CTCTCCACCG CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149669
    Clone ID Related Accession (Same CDS sequence) XM_021467333.1
    Accession Version XM_021467333.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GCTAAAACTC CCAAGGCCAA AATTGAACCA
    GCTGGTTTGA AGAAAGATGC TGAAGCTGCT AAGGAGAAAG CAAAACCTGC TCCAGTCAAG
    AAAGGGCCTG AGGTTTCCAG AGAAAAAGCC AAACCAGAGC CCGCAAAAAA AGAGCTTGAA
    GCGGTCAAAG AGAAAGCAAA ACCAGCACCA GCTAAAAAAG ATGAAGTCAA AGAGAAAGAA
    AAACCAGCTC CTGTAAAGAA AGCTGAAGAA ATTAAAAAGG CAAAAGCAGC TCCTTCAAGG
    AAAGAAGCTG TAAAGGAAAA ACCCAAACCT GTTTCCGAAA AGAAAGAACC TGCTGTTGCA
    GTTGAAAAAG CCAAGCCAAA ATCTACAAAG AAAGAAGCTG AAGTCGAGGA AAAAGTGAAA
    CCTGCTCCTG AGAAGAAAGA AGCTGAAGTC ATCATGGTGA AAACTAAACC AGTTCTTAAA
    AAGAAAGAGG CTGAAGTAAT CAAAGAGGTT AAACCAGCCC TTGAGAAGAA AGAGGCTGAA
    GTCATCAAGG AGAAAGTTAA ACCAGCTCCT GTAAAGAAAG AGCCTGAAGT TGTTAAAGAA
    AAGGAACCTG AACCAAAACC CAAAAGAGAG CCAGAAATTA TTAAGGAGAA ATCAGCACCA
    CCAAAGAAAG TTGAGGTTCC AAAACAAAAG GCTCCTGAAA AGAAAGCACC TGAAATAACC
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG CTGTAAAAGA GAAAGCTAAA
    CCAGAGAAGA AAGAGGCTGA AGCCGTAAAG GAGAAAACGA AACCAGCCCC TGAGAAAGAG
    ACTGAAATAA TCAAGGATAA AATTAAAGCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    AAGGAAGCAA AACCAGCTCC TGTAAAGAAA GAAACTGAAG TCATCAAAGA AAAAGAAAAA
    CCAGCTCCTG AGAAGAAAGA GGCTGAAGCT GTAAAGGAGA AAACGAAACC AACCTCTGAG
    AAGAAAGAGG CTGAAATAAT TAAGGAGAAA GTTAAAGCAG CTCCTGTGAA GAAAGAAGTT
    GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT GTAAAAAAAG AAACTGAAGT CATCAAAGAA
    AAAGAAAAAA CAGCTCCTGA GAAGAAAGAG GCTGAAGCTG TAAAGGAGAA AACGAAACCA
    ACCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGATAG TTAAACAAGC TCCTGTGAAG
    AAAGAGGCTG AACCTGTAAA GGAGATAACT AAACCAGCCC CTGAGAAGAA AGAGGCTGAA
    ATAATCAAGG AGAAAGTTAA AGCAGCACCT CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA
    CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC ATCAAACAAA AAGCCAAACC AGCTCCTGAG
    AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA GTTAAACCGG CTCCTGTAAA GATAGAGGCT
    AAACCTGTAA AGGAGATAAC TAAACCAGCC CCTGAGAAGA AAGAGGCTGA AATAATCAAG
    GAGAAGGTCA AATCAGCTCC TGTGAAGAAA GAAGTTGAAG TCAAGGAGGA AGCAAAACCA
    GCTCCTGAAA AGAAAGAGGC TGAAGTCATC AAGGAGAAAG TTAAACCTGC TCCTGTGAGG
    AAAGAAGCTG AAGAAATAAA GGAAAAAGCT GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA
    GTAGCCAAGG AGATAGTTAA ACCAACTCCT TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA
    AAGGCTCCTG AATCAAAACC CCAAAAAGAG GCTGAAATTA TCAAAGAGAA AGTTAAACCT
    GCACCTGTAA AGAAAGAGCC TGAAGTTGCA AAAGTACCAA AACCCAAAAA AGAGGCTGAA
    CTCGCCAAAG AGAAAGTTAA ACCTGCACCT GTAAAGAAAG AAGAAAAACC TAAGGCAGCA
    CCAAAGAAAG AGGCTGAAGC TATTAAGGAG AAACGTACAC CAGCTCCTGA AAAGAAAGTG
    GTAGAAGTCA TCAAGGAGAA AGTCAAACCA GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG
    AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA
    CCAAAACCCC AAAAAGAGGT TGAAGTCATC AAAGAGAAAG CTAAACCAGC CCTTAAGAAG
    AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT
    CCCAAAGAAA AGGTGCCTGA ACCAACACCA GAACCTGAGA GAGTTGAAGC TCCAAAAGAA
    AAGGCAAAAG TAATCCCTAG GGAAAAAGTA GCTGAGATTA CTAAAGAAAA ACCAAAGCCA
    GCACCAAAGA AAGAGGCTGA AGTAAAGGAG AAAGCTACAC CTGCTCCTGT GCACAAAGAG
    GTTGAAGTGT TCAAGGAGAA AGTTAAACAA GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCTAAA AAGACTGAAG TCATCAAAGC GAAAGTAAAA
    CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG AAGGAGAAAA TTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATAAA GGAGAGAATT AAACCTGCTC CTGTAAAGAA AGAGCCTGAA
    GTTCCTAAAG AAAAGGCTCC TGAACCAAAA CCCAAAAAAG AACCTGAAGT TATCAAAGAG
    AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA CCCGAAATAA CCAAAGAAAA ACCAAAGCCA
    GCAGCAAAGA AAGTAGCTGA AGTCATCAAA GACAGAACTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAACCAAAA GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC
    ATTGAAAAGA AAGTTATACC AGCTCCCGTA AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG
    GCTCCTGAAC TGACAACAAA AAAAGCACCT GAGATAACCA AAGAAAAACC GAAGCCAGTA
    CCAAAGAAAG AGGCTGAAGT CATTAAAGAG AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG
    GTTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT
    AAGGAAAAAG CACCTGAACC AAAATCAGAA CCTACAAAAA AAGTTGAGGT TCCAAAGGAA
    AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA CCTGAAATAA CCAAAGAAAA GCCAAAACCA
    GCACCAAAGA AAGAAGCTGT AGTCATCAAA GAAAAAGCTG TACCAGCCAT CCTGAAGGAA
    GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT CCTGAGAAGA AAGTGGCTGA AATTAAGGAG
    AAAGTTAAAC CAGCTTCTAT AATGAAAGAG CCTGAAGTTG TGAAACAAAA GACTCCTGAA
    CCGAAACCCA AAAAAGAGCC TGAAGTTGTG AAAGAAAAGG CTCCTGAACC TAAACCCAAA
    AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC AAACCAGCAC CTCAGAAGAA AGAAGTCATC
    AAAGAAAAAG TCAAACTTGC TCCTGTAAAG AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA
    CTTAAACCAG AACCCAAAAA AGATGAAGTT GGTCTTATTG GTCTGGACCT GGAGCTCCTA
    AACTCCATCA ACCAAAAACT GTCTCTCCTG GACTTGCTAA GGAAGGACAT TGGAGAAATG
    AAAAGCGATC TGGAGTCCAC TCAGAATCAA ATCACACTTT TACGGATGGA CAACAGAACG
    ATCAAAGAGC CAGAGACTAT TGGTGTGAAA GTAAAACCAG CAGTCAAGGA AAAAGCAAAA
    AGAGACCACG CTCTGATCAA TGCTACTAGG ACTGCACATA TTAAAACAGA GCGTGAATCT
    CTAAAAAACA TCACGAAACC AGTGCCTGCA AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG
    ATTGCGCTGG AGCCTAAAAA GAAAGGAATC ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA
    ACTTTACAGA AAGAACCTGA GTTGGTCAAA GATAAAGTAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323008.1
    CDS338..8413
    Translation

    Target ORF information:

    RefSeq Version XM_021467333.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GCTAAAACTC CCAAGGCCAA AATTGAACCA
    GCTGGTTTGA AGAAAGATGC TGAAGCTGCT AAGGAGAAAG CAAAACCTGC TCCAGTCAAG
    AAAGGGCCTG AGGTTTCCAG AGAAAAAGCC AAACCAGAGC CCGCAAAAAA AGAGCTTGAA
    GCGGTCAAAG AGAAAGCAAA ACCAGCACCA GCTAAAAAAG ATGAAGTCAA AGAGAAAGAA
    AAACCAGCTC CTGTAAAGAA AGCTGAAGAA ATTAAAAAGG CAAAAGCAGC TCCTTCAAGG
    AAAGAAGCTG TAAAGGAAAA ACCCAAACCT GTTTCCGAAA AGAAAGAACC TGCTGTTGCA
    GTTGAAAAAG CCAAGCCAAA ATCTACAAAG AAAGAAGCTG AAGTCGAGGA AAAAGTGAAA
    CCTGCTCCTG AGAAGAAAGA AGCTGAAGTC ATCATGGTGA AAACTAAACC AGTTCTTAAA
    AAGAAAGAGG CTGAAGTAAT CAAAGAGGTT AAACCAGCCC TTGAGAAGAA AGAGGCTGAA
    GTCATCAAGG AGAAAGTTAA ACCAGCTCCT GTAAAGAAAG AGCCTGAAGT TGTTAAAGAA
    AAGGAACCTG AACCAAAACC CAAAAGAGAG CCAGAAATTA TTAAGGAGAA ATCAGCACCA
    CCAAAGAAAG TTGAGGTTCC AAAACAAAAG GCTCCTGAAA AGAAAGCACC TGAAATAACC
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG CTGTAAAAGA GAAAGCTAAA
    CCAGAGAAGA AAGAGGCTGA AGCCGTAAAG GAGAAAACGA AACCAGCCCC TGAGAAAGAG
    ACTGAAATAA TCAAGGATAA AATTAAAGCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    AAGGAAGCAA AACCAGCTCC TGTAAAGAAA GAAACTGAAG TCATCAAAGA AAAAGAAAAA
    CCAGCTCCTG AGAAGAAAGA GGCTGAAGCT GTAAAGGAGA AAACGAAACC AACCTCTGAG
    AAGAAAGAGG CTGAAATAAT TAAGGAGAAA GTTAAAGCAG CTCCTGTGAA GAAAGAAGTT
    GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT GTAAAAAAAG AAACTGAAGT CATCAAAGAA
    AAAGAAAAAA CAGCTCCTGA GAAGAAAGAG GCTGAAGCTG TAAAGGAGAA AACGAAACCA
    ACCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGATAG TTAAACAAGC TCCTGTGAAG
    AAAGAGGCTG AACCTGTAAA GGAGATAACT AAACCAGCCC CTGAGAAGAA AGAGGCTGAA
    ATAATCAAGG AGAAAGTTAA AGCAGCACCT CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA
    CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC ATCAAACAAA AAGCCAAACC AGCTCCTGAG
    AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA GTTAAACCGG CTCCTGTAAA GATAGAGGCT
    AAACCTGTAA AGGAGATAAC TAAACCAGCC CCTGAGAAGA AAGAGGCTGA AATAATCAAG
    GAGAAGGTCA AATCAGCTCC TGTGAAGAAA GAAGTTGAAG TCAAGGAGGA AGCAAAACCA
    GCTCCTGAAA AGAAAGAGGC TGAAGTCATC AAGGAGAAAG TTAAACCTGC TCCTGTGAGG
    AAAGAAGCTG AAGAAATAAA GGAAAAAGCT GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA
    GTAGCCAAGG AGATAGTTAA ACCAACTCCT TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA
    AAGGCTCCTG AATCAAAACC CCAAAAAGAG GCTGAAATTA TCAAAGAGAA AGTTAAACCT
    GCACCTGTAA AGAAAGAGCC TGAAGTTGCA AAAGTACCAA AACCCAAAAA AGAGGCTGAA
    CTCGCCAAAG AGAAAGTTAA ACCTGCACCT GTAAAGAAAG AAGAAAAACC TAAGGCAGCA
    CCAAAGAAAG AGGCTGAAGC TATTAAGGAG AAACGTACAC CAGCTCCTGA AAAGAAAGTG
    GTAGAAGTCA TCAAGGAGAA AGTCAAACCA GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG
    AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA
    CCAAAACCCC AAAAAGAGGT TGAAGTCATC AAAGAGAAAG CTAAACCAGC CCTTAAGAAG
    AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT
    CCCAAAGAAA AGGTGCCTGA ACCAACACCA GAACCTGAGA GAGTTGAAGC TCCAAAAGAA
    AAGGCAAAAG TAATCCCTAG GGAAAAAGTA GCTGAGATTA CTAAAGAAAA ACCAAAGCCA
    GCACCAAAGA AAGAGGCTGA AGTAAAGGAG AAAGCTACAC CTGCTCCTGT GCACAAAGAG
    GTTGAAGTGT TCAAGGAGAA AGTTAAACAA GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCTAAA AAGACTGAAG TCATCAAAGC GAAAGTAAAA
    CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG AAGGAGAAAA TTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATAAA GGAGAGAATT AAACCTGCTC CTGTAAAGAA AGAGCCTGAA
    GTTCCTAAAG AAAAGGCTCC TGAACCAAAA CCCAAAAAAG AACCTGAAGT TATCAAAGAG
    AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA CCCGAAATAA CCAAAGAAAA ACCAAAGCCA
    GCAGCAAAGA AAGTAGCTGA AGTCATCAAA GACAGAACTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAACCAAAA GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC
    ATTGAAAAGA AAGTTATACC AGCTCCCGTA AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG
    GCTCCTGAAC TGACAACAAA AAAAGCACCT GAGATAACCA AAGAAAAACC GAAGCCAGTA
    CCAAAGAAAG AGGCTGAAGT CATTAAAGAG AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG
    GTTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT
    AAGGAAAAAG CACCTGAACC AAAATCAGAA CCTACAAAAA AAGTTGAGGT TCCAAAGGAA
    AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA CCTGAAATAA CCAAAGAAAA GCCAAAACCA
    GCACCAAAGA AAGAAGCTGT AGTCATCAAA GAAAAAGCTG TACCAGCCAT CCTGAAGGAA
    GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT CCTGAGAAGA AAGTGGCTGA AATTAAGGAG
    AAAGTTAAAC CAGCTTCTAT AATGAAAGAG CCTGAAGTTG TGAAACAAAA GACTCCTGAA
    CCGAAACCCA AAAAAGAGCC TGAAGTTGTG AAAGAAAAGG CTCCTGAACC TAAACCCAAA
    AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC AAACCAGCAC CTCAGAAGAA AGAAGTCATC
    AAAGAAAAAG TCAAACTTGC TCCTGTAAAG AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA
    CTTAAACCAG AACCCAAAAA AGATGAAGTT GGTCTTATTG GTCTGGACCT GGAGCTCCTA
    AACTCCATCA ACCAAAAACT GTCTCTCCTG GACTTGCTAA GGAAGGACAT TGGAGAAATG
    AAAAGCGATC TGGAGTCCAC TCAGAATCAA ATCACACTTT TACGGATGGA CAACAGAACG
    ATCAAAGAGC CAGAGACTAT TGGTGTGAAA GTAAAACCAG CAGTCAAGGA AAAAGCAAAA
    AGAGACCACG CTCTGATCAA TGCTACTAGG ACTGCACATA TTAAAACAGA GCGTGAATCT
    CTAAAAAACA TCACGAAACC AGTGCCTGCA AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG
    ATTGCGCTGG AGCCTAAAAA GAAAGGAATC ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA
    ACTTTACAGA AAGAACCTGA GTTGGTCAAA GATAAAGTAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149670
    Clone ID Related Accession (Same CDS sequence) XM_021467334.1
    Accession Version XM_021467334.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GCTGAGATTA CTAAAGAAAA ACCAAAGCCA
    GCACCAAAGA AAGAGGCTGA AGTAAAGGAG AAAGCTACAC CTGCTCCTGT GCACAAAGAG
    GTTGAAGTGT TCAAGGAGAA AGTTAAACAA GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCTAAA AAGACTGAAG TCATCAAAGC GAAAGTAAAA
    CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG AAGGAGAAAA TTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATAAA GGAGAGAATT AAACCTGCTC CTGTAAAGAA AGAGCCTGAA
    GTTCCTAAAG AAAAGGCTCC TGAACCAAAA CCCAAAAAAG AACCTGAAGT TATCAAAGAG
    AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA CCCGAAATAA CCAAAGAAAA ACCAAAGCCA
    GCAGCAAAGA AAGTAGCTGA AGTCATCAAA GACAGAACTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAACCAAAA GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC
    ATTGAAAAGA AAGTTATACC AGCTCCCGTA AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG
    GCTCCTGAAC TGACAACAAA AAAAGCACCT GAGATAACCA AAGAAAAACC GAAGCCAGTA
    CCAAAGAAAG AGGCTGAAGT CATTAAAGAG AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG
    GTTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT
    AAGGAAAAAG CACCTGAACC AAAATCAGAA CCTACAAAAA AAGTTGAGGT TCCAAAGGAA
    AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA CCTGAAATAA CCAAAGAAAA GCCAAAACCA
    GCACCAAAGA AAGAAGCTGT AGTCATCAAA GAAAAAGCTG TACCAGCCAT CCTGAAGGAA
    GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT CCTGAGAAGA AAGTGGCTGA AATTAAGGAG
    AAAGTTAAAC CAGCTTCTAT AATGAAAGAG CCTGAAGTTG TGAAACAAAA GACTCCTGAA
    CCGAAACCCA AAAAAGAGCC TGAAGTTGTG AAAGAAAAGG CTCCTGAACC TAAACCCAAA
    AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC AAACCAGCAC CTCAGAAGAA AGAAGTCATC
    AAAGAAAAAG TCAAACTTGC TCCTGTAAAG AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA
    CTTAAACCAG AACCCAAAAA AGATGAAGTT GGTCTTATTG GTCTGGACCT GGAGCTCCTA
    AACTCCATCA ACCAAAAACT GTCTCTCCTG GACTTGCTAA GGAAGGACAT TGGAGAAATG
    AAAAGCGATC TGGAGTCCAC TCAGAATCAA ATCACACTTT TACGGATGGA CAACAGAACG
    ATCAAAGAGC CAGAGACTAT TGGTGTGAAA GTAAAACCAG CAGTCAAGGA AAAAGCAAAA
    AGAGACCACG CTCTGATCAA TGCTACTAGG ACTGCACATA TTAAAACAGA GCGTGAATCT
    CTAAAAAACA TCACGAAACC AGTGCCTGCA AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG
    ATTGCGCTGG AGCCTAAAAA GAAAGGAATC ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA
    ACTTTACAGA AAGAACCTGA GTTGGTCAAA GATAAAGTAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323009.1
    CDS338..8413
    Translation

    Target ORF information:

    RefSeq Version XM_021467334.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GCTGAGATTA CTAAAGAAAA ACCAAAGCCA
    GCACCAAAGA AAGAGGCTGA AGTAAAGGAG AAAGCTACAC CTGCTCCTGT GCACAAAGAG
    GTTGAAGTGT TCAAGGAGAA AGTTAAACAA GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCTAAA AAGACTGAAG TCATCAAAGC GAAAGTAAAA
    CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG AAGGAGAAAA TTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATAAA GGAGAGAATT AAACCTGCTC CTGTAAAGAA AGAGCCTGAA
    GTTCCTAAAG AAAAGGCTCC TGAACCAAAA CCCAAAAAAG AACCTGAAGT TATCAAAGAG
    AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA CCCGAAATAA CCAAAGAAAA ACCAAAGCCA
    GCAGCAAAGA AAGTAGCTGA AGTCATCAAA GACAGAACTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAACCAAAA GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC
    ATTGAAAAGA AAGTTATACC AGCTCCCGTA AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG
    GCTCCTGAAC TGACAACAAA AAAAGCACCT GAGATAACCA AAGAAAAACC GAAGCCAGTA
    CCAAAGAAAG AGGCTGAAGT CATTAAAGAG AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG
    GTTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT
    AAGGAAAAAG CACCTGAACC AAAATCAGAA CCTACAAAAA AAGTTGAGGT TCCAAAGGAA
    AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA CCTGAAATAA CCAAAGAAAA GCCAAAACCA
    GCACCAAAGA AAGAAGCTGT AGTCATCAAA GAAAAAGCTG TACCAGCCAT CCTGAAGGAA
    GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT CCTGAGAAGA AAGTGGCTGA AATTAAGGAG
    AAAGTTAAAC CAGCTTCTAT AATGAAAGAG CCTGAAGTTG TGAAACAAAA GACTCCTGAA
    CCGAAACCCA AAAAAGAGCC TGAAGTTGTG AAAGAAAAGG CTCCTGAACC TAAACCCAAA
    AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC AAACCAGCAC CTCAGAAGAA AGAAGTCATC
    AAAGAAAAAG TCAAACTTGC TCCTGTAAAG AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA
    CTTAAACCAG AACCCAAAAA AGATGAAGTT GGTCTTATTG GTCTGGACCT GGAGCTCCTA
    AACTCCATCA ACCAAAAACT GTCTCTCCTG GACTTGCTAA GGAAGGACAT TGGAGAAATG
    AAAAGCGATC TGGAGTCCAC TCAGAATCAA ATCACACTTT TACGGATGGA CAACAGAACG
    ATCAAAGAGC CAGAGACTAT TGGTGTGAAA GTAAAACCAG CAGTCAAGGA AAAAGCAAAA
    AGAGACCACG CTCTGATCAA TGCTACTAGG ACTGCACATA TTAAAACAGA GCGTGAATCT
    CTAAAAAACA TCACGAAACC AGTGCCTGCA AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG
    ATTGCGCTGG AGCCTAAAAA GAAAGGAATC ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA
    ACTTTACAGA AAGAACCTGA GTTGGTCAAA GATAAAGTAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149671
    Clone ID Related Accession (Same CDS sequence) XM_021467335.1
    Accession Version XM_021467335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGAAGTC
    ATCAAAGAAA AAGTCAAACT TGCTCCTGTA AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG
    GCACTTAAAC CAGAACCCAA AAAAGATGAA GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC
    CTAAACTCCA TCAACCAAAA ACTGTCTCTC CTGGACTTGC TAAGGAAGGA CATTGGAGAA
    ATGAAAAGCG ATCTGGAGTC CACTCAGAAT CAAATCACAC TTTTACGGAT GGACAACAGA
    ACGATCAAAG AGCCAGAGAC TATTGGTGTG AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA
    AAAAGAGACC ACGCTCTGAT CAATGCTACT AGGACTGCAC ATATTAAAAC AGAGCGTGAA
    TCTCTAAAAA ACATCACGAA ACCAGTGCCT GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT
    AAGATTGCGC TGGAGCCTAA AAAGAAAGGA ATCATCCCTG AGAAGGTCAA AGAAGTAAAG
    GAAACTTTAC AGAAAGAACC TGAGTTGGTC AAAGATAAAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323010.1
    CDS338..8413
    Translation

    Target ORF information:

    RefSeq Version XM_021467335.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGAAGTC
    ATCAAAGAAA AAGTCAAACT TGCTCCTGTA AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG
    GCACTTAAAC CAGAACCCAA AAAAGATGAA GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC
    CTAAACTCCA TCAACCAAAA ACTGTCTCTC CTGGACTTGC TAAGGAAGGA CATTGGAGAA
    ATGAAAAGCG ATCTGGAGTC CACTCAGAAT CAAATCACAC TTTTACGGAT GGACAACAGA
    ACGATCAAAG AGCCAGAGAC TATTGGTGTG AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA
    AAAAGAGACC ACGCTCTGAT CAATGCTACT AGGACTGCAC ATATTAAAAC AGAGCGTGAA
    TCTCTAAAAA ACATCACGAA ACCAGTGCCT GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT
    AAGATTGCGC TGGAGCCTAA AAAGAAAGGA ATCATCCCTG AGAAGGTCAA AGAAGTAAAG
    GAAACTTTAC AGAAAGAACC TGAGTTGGTC AAAGATAAAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149672
    Clone ID Related Accession (Same CDS sequence) XM_021467336.1
    Accession Version XM_021467336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGA TGCTCCGGCT AGAGCAGTGC ATCCAGAATC CTTTACAGCC 
    AGGAGTCATG GAATTTTACT AACCATGAGT CGTCCAGCAC AATGGGTGGT CATCATCACA
    CTTCTGATCT CTTGGTCAAT AGCTGGTGTT GTCATGTTTG ACTTTGTCAG TGATGATCAG
    ATTGCAAGCA TCCAGGATTT AGGTTCAGAT CCAGCGCTAG CAGTGAACAT GGCCATTGAA
    GGAATCGAAA ACAGGATAAA CCACATGAAT AATATGTTTA ATGATGCTAA TGAATACATT
    GGTGAATTAT TATATAATCC GAAGGAAGCT CTTTATTCAG CAGCTGACTC ATCCGTGTCT
    TATGTGACAG AAATGCTCAC ACCTGATGAA TTAATCATCG AAACTGTGAA ATATGGTGGA
    ACTGCATTGG ATGAGGTTAC AGAATGGGTC AGATTTCCAG TCTGCTGCTT ATTCCACTTA
    TACGAAGACG TCCTGGATAT AGTCTTTACG CCAGTGGATT TGCTGAGTGA AGCTGTTGTG
    CAAATTTTAA GTGGAATCAA GACTATTGTA CAGTATCTTT CAAACATATT GGAAGGCGCT
    AGAGACATCA CTGATATTGC CAAACTTGGA GGCACTGTAT TGGATAAGGT TACTGACTGT
    ATTCGATTAC CAGTGGGTTA CTTATTTTAC TTATTTGAAG CCATCCTGGA GGTAACCATT
    ATTTATCCAG CGGATTTGAC AAGCGAAGTA TTTCTGCAAA TTTTAAATGT GATCAAGAAC
    ACTTTATCAG CCATATTTGG GTTCTTCAAA GGGGATGCAG ACATCACTGA AACAGCCAAA
    CTTGGAGGCA CTGTATTGGA TACAGTTACT GACTGGATTC GATTGCCAGT GGGTTACTTA
    TTTTACTTGT TTGAAGCCAT TCTGGAGGTA ACCATTATTT ATCCAGCGGA TTTGGCAAGT
    GAAGCATTCC TACAAATTTT AAATGTGATC AAGAACACTT TATCAGCCAT ATTTGGGTTC
    CTCAAAGGGG ATGCAGACAT CACTGAAACA GCCAAACTTG GAGGCACTGT ATTGGATACA
    GTTACTGACT GGATTCGATT GCCAGTGGGA TACTTATTTT ATTTATTTGA AGCCATCCTG
    GAGGTAACCA TTATTTATCC AGCAGATATG GCAAGTGAAG CATTTTTGCA AATTTTAAAT
    GTGATCAAGA ACACTTCATC AGCCATATTT GGGTTTCTTG AAGCTTGGTT CCCCAAAATG
    AGCACTCGTC CTACAGAGCT GGTTGAAAAG GTTGCAGAGG AGGCCACTGA TGTAAAGACC
    ACCGTCTCTA ATTATCTGTC CAGTCTGTTT GTGAGGGATG AAGGTGGTAT TCCTGACGTC
    ACTTTTGATC CCATGAAAGT TGTCTCAGAC ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT
    ATGTTATTAG CTTATCTCTC AAACATGCTC ATGGAAGACA AAGTTTCAGA TGAAACACCA
    CAGGTGATAA GGCGAAAAGG AGAATTTCTG CCCCCTATGG AAAAAGTTGC AGAAGTACTA
    GGAAAAACTC AGGACCCCAT CTACGCTGCC TACATCGCTG TGAGATCCAC AGCTGAGACA
    GACGGCGATG AACTGGAAAT AAAGGGGGCT GAGGTGGACT CTGCTGAAGA TATCTTGGAA
    GAACAAAAAG TTGAAAAGAC AGTGGACGGC TCTGAAAATG ATGCAGTTGC CTCTGTTGAG
    GAGGAAAAAG CAGTTGTTGA TATTGTAGAT GAGGAATCTT CTCATACGCC TGAAGTCACA
    GATGAAGAAA TGGCAAAAGA AAAAGATGAA GACACTTCTG ATTCAACTGA AATGACTTCT
    GATGTGCAAG ACATTGATGA TACCATAGAT TCTGAGACTG AAGAAATTCC TGAAATGGTT
    GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA CCTTTTGTAA TTGAAAAGGT TGAAGACACC
    TCTGATTCGA AAGAAATCAC TTCTAATGCC GCAGATGTGC AAGATGTTGA TGATAATTCT
    GATGTTGATA CTGAAGAAAT TCCTGAATTG GTTGGAGGTG TTTCAGATGT TGGTGAAGAG
    GAACCTTCTG AAGTCATAGA TGAAGAAATT GCAACTGAAA AGCTTGAAGA CGCCTCTGAT
    TCAACTGGAT TGATTCCCAG TGCTGAAAAT GTGAAAGACA CTGACGATAT CTTAGAAGTT
    GACGCAGAGG TGAATGAAGT TGAAACAGTT GAAGACATTT CAGAAGTTAC AAATGAGGAA
    ACTTCTGATG TGCCAGAAGT CATAGATGAG GAAAAGGTTG AAAAGTCTGA TGATATCTTT
    CATTTTGCAG AGGACAAACC AACTGAATTG ATTTCTGATG CTCCAGATGT AACAGAAGAC
    ATTGAAATCT CAATCTCTGC AGTTTCAGAG GATAATGAAA CAATTCAAGA AGCTGATATG
    ACAGATGTCA AAGATGAGGA AATGGAAGCT GAAAAGGTTG ATGAAATCTC CTATTTTGTA
    GAGGATAAAT CAACTGAAAT TACTTCTGAT GCTACAGATG TGAAAGAAGA CACTGATGGC
    TTTACAGTTA CAGAGGAGAA AGGAACAATT GAAAGCCTTC AAGAAGCAGC AATGAAAATG
    TCGGGAAATG CAGATGATGA AATCCCCAAT GTCACAGAGA TGGTTGAAGA ACCTGATACT
    TCTGATGTTA TGGATGAGCT AGACGAACCT GAAAAAGATG AAGATGTAAT CAGTGTCACC
    AATGAAAGGG TTACAACTGC ACAAGATGTC ATATTTTCAA AGGAAAAAAT GGGAGCAGCA
    GAACTGGATG ATCATGACAA ACTCAGGGCT AAGGATGATT CAGAAGTGGA TCACATGGAG
    AAGGAAATTG CAGATGCTGA AAATGACCTT CAGAATGAGA TAGATAAAGA TGAGTTTAAG
    GTTGAAGATG CATCTCATCA AACTATTTTG GGAAGCACTG CTGCTGAAGA AGACCAAGAT
    GATGATGATG ATGATGAAGA ACCTGTCCTT CTTACTGTCA AACATGAGCT CACAGACGGT
    GATGATGAAA ATAATAATAA CAACGACAGA AAGAGAGATG CCAGAACTAA AGGTCTGATC
    AATAGAAAAA CACCAAAGAG CCATTCCACT GAAACTGATG AACAAAAGGC GCACAAGGAT
    CTTCATAAAG AGTCTGCTAA AGTTCTTGAG CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT
    AGAGAGGAGA TATACAAAAT CATTCAAGAA ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG
    GAAGAGATCG TGTTACAACA GATAAACATA ACAGTAGAAA AGGTCAAGAA AGAAGAAACT
    GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA GAAAAGGCTC ATAAACCAAA GGATGCAAAG
    ATTGCCAAAG ACAAACCTAA AGCAGTCCCA GTAAAGCAAG AGGATGAAGT AGCCAAGAAA
    GAAACCAAAC CAGCACCATC TAAGAAAGAG GCTAAAATTG TGAAGGAGAA ACCCAAAGCA
    CATCCTGCAA AGAAAGAAAA AGCTAAAGAT ATTAAGGAAA AGGCAAAATC TGGTCAAAAA
    GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA
    ATTGATGCCA AAGTCAAACC AGCAGCAGAA AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA
    CCTAAAGTGA AGAAAGAGGC TGAAGTAATC AAAAAGATTA AACTAGAGCC TGAGAGGAAA
    GAAGCTAAAA CTCCCAAGGC CAAAATTGAA CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT
    GCTAAGGAGA AAGCAAAACC TGCTCCAGTC AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA
    GCCAAACCAG AGCCCGCAAA AAAAGAGCTT GAAGCGGTCA AAGAGAAAGC AAAACCAGCA
    CCAGCTAAAA AAGATGAAGT CAAAGAGAAA GAAAAACCAG CTCCTGTAAA GAAAGCTGAA
    GAAATTAAAA AGGCAAAAGC AGCTCCTTCA AGGAAAGAAG CTGTAAAGGA AAAACCCAAA
    CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT GCAGTTGAAA AAGCCAAGCC AAAATCTACA
    AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG AAACCTGCTC CTGAGAAGAA AGAAGCTGAA
    GTCATCATGG TGAAAACTAA ACCAGTTCTT AAAAAGAAAG AGGCTGAAGT AATCAAAGAG
    GTTAAACCAG CCCTTGAGAA GAAAGAGGCT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA GAAAAGGAAC CTGAACCAAA ACCCAAAAGA
    GAGCCAGAAA TTATTAAGGA GAAATCAGCA CCACCAAAGA AAGTTGAGGT TCCAAAACAA
    AAGGCTCCTG AAAAGAAAGC ACCTGAAATA ACCAAAGAAA AACCAAAGCC AGCACCAAAG
    AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT AAACCAGAGA AGAAAGAGGC TGAAGCCGTA
    AAGGAGAAAA CGAAACCAGC CCCTGAGAAA GAGACTGAAA TAATCAAGGA TAAAATTAAA
    GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC AAGAAGGAAG CAAAACCAGC TCCTGTAAAG
    AAAGAAACTG AAGTCATCAA AGAAAAAGAA AAACCAGCTC CTGAGAAGAA AGAGGCTGAA
    GCTGTAAAGG AGAAAACGAA ACCAACCTCT GAGAAGAAAG AGGCTGAAAT AATTAAGGAG
    AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA GTTGAAGTCA AGGAGGAAGC AAAACCAGCT
    CCTGTAAAAA AAGAAACTGA AGTCATCAAA GAAAAAGAAA AAACAGCTCC TGAGAAGAAA
    GAGGCTGAAG CTGTAAAGGA GAAAACGAAA CCAACCCCTG AGAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA TAGTTAAACA AGCTCCTGTG AAGAAAGAGG CTGAACCTGT AAAGGAGATA
    ACTAAACCAG CCCCTGAGAA GAAAGAGGCT GAAATAATCA AGGAGAAAGT TAAAGCAGCA
    CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA AAACCAGCTC CTGTAAAGAA AGAAGCTGAA
    GTCATCAAAC AAAAAGCCAA ACCAGCTCCT GAGAAGAAAG AGGCCAAAGT CATCAAGGAG
    AAAGTTAAAC CGGCTCCTGT AAAGATAGAG GCTAAACCTG TAAAGGAGAT AACTAAACCA
    GCCCCTGAGA AGAAAGAGGC TGAAATAATC AAGGAGAAGG TCAAATCAGC TCCTGTGAAG
    AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA AAGTTAAACC TGCTCCTGTG AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA
    GCTGAAGCAG TTTATGAGAA AAAAGAGGCT GAAGTAGCCA AGGAGATAGT TAAACCAACT
    CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG GAAAAGGCTC CTGAATCAAA ACCCCAAAAA
    GAGGCTGAAA TTATCAAAGA GAAAGTTAAA CCTGCACCTG TAAAGAAAGA GCCTGAAGTT
    GCAAAAGTAC CAAAACCCAA AAAAGAGGCT GAACTCGCCA AAGAGAAAGT TAAACCTGCA
    CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA GCACCAAAGA AAGAGGCTGA AGCTATTAAG
    GAGAAACGTA CACCAGCTCC TGAAAAGAAA GTGGTAGAAG TCATCAAGGA GAAAGTCAAA
    CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG GAGAAAGTTA GACCTGCTCC TGAAAAGAAA
    GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT GAACCAAAAC CCCAAAAAGA GGTTGAAGTC
    ATCAAAGAGA AAGCTAAACC AGCCCTTAAG AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT
    CAACCAGCTC CTATAAAGAA AGAGCCAGAG CTTCCCAAAG AAAAGGTGCC TGAACCAACA
    CCAGAACCTG AGAGAGTTGA AGCTCCAAAA GAAAAGGCAA AAGTAATCCC TAGGGAAAAA
    GTAGCTGAGA TTACTAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGTAAAG
    GAGAAAGCTA CACCTGCTCC TGTGCACAAA GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA
    CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCT
    AAAAAGACTG AAGTCATCAA AGCGAAAGTA AAACCAGCCC CTGAGAAAAA AGAGGCTGAA
    GTGAAGGAGA AAATTAAACC AGTCCCTGAG AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA
    ATTAAACCTG CTCCTGTAAA GAAAGAGCCT GAAGTTCCTA AAGAAAAGGC TCCTGAACCA
    AAACCCAAAA AAGAACCTGA AGTTATCAAA GAGAAAGTGA AACCAGCGCC AGAGAAGAAA
    GCACCCGAAA TAACCAAAGA AAAACCAAAG CCAGCAGCAA AGAAAGTAGC TGAAGTCATC
    AAAGACAGAA CTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGAAACCA
    AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA GTCATTGAAA AGAAAGTTAT ACCAGCTCCC
    GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA AAGGCTCCTG AACTGACAAC AAAAAAAGCA
    CCTGAGATAA CCAAAGAAAA ACCGAAGCCA GTACCAAAGA AAGAGGCTGA AGTCATTAAA
    GAGAAAGCTA AACTAGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAGGA GAAAGTTAAA
    CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT CATAAGGAAA AAGCACCTGA ACCAAAATCA
    GAACCTACAA AAAAAGTTGA GGTTCCAAAG GAAAAGGCAA AACCAGTTGC TAAAGAAAAG
    GAACCTGAAA TAACCAAAGA AAAGCCAAAA CCAGCACCAA AGAAAGAAGC TGTAGTCATC
    AAAGAAAAAG CTGTACCAGC CATCCTGAAG GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA
    GCTCCTGAGA AGAAAGTGGC TGAAATTAAG GAGAAAGTTA AACCAGCTTC TATAATGAAA
    GAGCCTGAAG TTGTGAAACA AAAGACTCCT GAACCGAAAC CCAAAAAAGA GCCTGAAGTT
    GTGAAAGAAA AGGCTCCTGA ACCTAAACCC AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA
    GCCAAACCAG CACCTCAGAA GAAAGAAGTC ATCAAAGAAA AAGTCAAACT TGCTCCTGTA
    AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG GCACTTAAAC CAGAACCCAA AAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323011.1
    CDS338..8386
    Translation

    Target ORF information:

    RefSeq Version XM_021467336.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCTCAGG CAACCAAAGA TGCTCCGGCT AGAGCAGTGC ATCCAGAATC CTTTACAGCC 
    AGGAGTCATG GAATTTTACT AACCATGAGT CGTCCAGCAC AATGGGTGGT CATCATCACA
    CTTCTGATCT CTTGGTCAAT AGCTGGTGTT GTCATGTTTG ACTTTGTCAG TGATGATCAG
    ATTGCAAGCA TCCAGGATTT AGGTTCAGAT CCAGCGCTAG CAGTGAACAT GGCCATTGAA
    GGAATCGAAA ACAGGATAAA CCACATGAAT AATATGTTTA ATGATGCTAA TGAATACATT
    GGTGAATTAT TATATAATCC GAAGGAAGCT CTTTATTCAG CAGCTGACTC ATCCGTGTCT
    TATGTGACAG AAATGCTCAC ACCTGATGAA TTAATCATCG AAACTGTGAA ATATGGTGGA
    ACTGCATTGG ATGAGGTTAC AGAATGGGTC AGATTTCCAG TCTGCTGCTT ATTCCACTTA
    TACGAAGACG TCCTGGATAT AGTCTTTACG CCAGTGGATT TGCTGAGTGA AGCTGTTGTG
    CAAATTTTAA GTGGAATCAA GACTATTGTA CAGTATCTTT CAAACATATT GGAAGGCGCT
    AGAGACATCA CTGATATTGC CAAACTTGGA GGCACTGTAT TGGATAAGGT TACTGACTGT
    ATTCGATTAC CAGTGGGTTA CTTATTTTAC TTATTTGAAG CCATCCTGGA GGTAACCATT
    ATTTATCCAG CGGATTTGAC AAGCGAAGTA TTTCTGCAAA TTTTAAATGT GATCAAGAAC
    ACTTTATCAG CCATATTTGG GTTCTTCAAA GGGGATGCAG ACATCACTGA AACAGCCAAA
    CTTGGAGGCA CTGTATTGGA TACAGTTACT GACTGGATTC GATTGCCAGT GGGTTACTTA
    TTTTACTTGT TTGAAGCCAT TCTGGAGGTA ACCATTATTT ATCCAGCGGA TTTGGCAAGT
    GAAGCATTCC TACAAATTTT AAATGTGATC AAGAACACTT TATCAGCCAT ATTTGGGTTC
    CTCAAAGGGG ATGCAGACAT CACTGAAACA GCCAAACTTG GAGGCACTGT ATTGGATACA
    GTTACTGACT GGATTCGATT GCCAGTGGGA TACTTATTTT ATTTATTTGA AGCCATCCTG
    GAGGTAACCA TTATTTATCC AGCAGATATG GCAAGTGAAG CATTTTTGCA AATTTTAAAT
    GTGATCAAGA ACACTTCATC AGCCATATTT GGGTTTCTTG AAGCTTGGTT CCCCAAAATG
    AGCACTCGTC CTACAGAGCT GGTTGAAAAG GTTGCAGAGG AGGCCACTGA TGTAAAGACC
    ACCGTCTCTA ATTATCTGTC CAGTCTGTTT GTGAGGGATG AAGGTGGTAT TCCTGACGTC
    ACTTTTGATC CCATGAAAGT TGTCTCAGAC ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT
    ATGTTATTAG CTTATCTCTC AAACATGCTC ATGGAAGACA AAGTTTCAGA TGAAACACCA
    CAGGTGATAA GGCGAAAAGG AGAATTTCTG CCCCCTATGG AAAAAGTTGC AGAAGTACTA
    GGAAAAACTC AGGACCCCAT CTACGCTGCC TACATCGCTG TGAGATCCAC AGCTGAGACA
    GACGGCGATG AACTGGAAAT AAAGGGGGCT GAGGTGGACT CTGCTGAAGA TATCTTGGAA
    GAACAAAAAG TTGAAAAGAC AGTGGACGGC TCTGAAAATG ATGCAGTTGC CTCTGTTGAG
    GAGGAAAAAG CAGTTGTTGA TATTGTAGAT GAGGAATCTT CTCATACGCC TGAAGTCACA
    GATGAAGAAA TGGCAAAAGA AAAAGATGAA GACACTTCTG ATTCAACTGA AATGACTTCT
    GATGTGCAAG ACATTGATGA TACCATAGAT TCTGAGACTG AAGAAATTCC TGAAATGGTT
    GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA CCTTTTGTAA TTGAAAAGGT TGAAGACACC
    TCTGATTCGA AAGAAATCAC TTCTAATGCC GCAGATGTGC AAGATGTTGA TGATAATTCT
    GATGTTGATA CTGAAGAAAT TCCTGAATTG GTTGGAGGTG TTTCAGATGT TGGTGAAGAG
    GAACCTTCTG AAGTCATAGA TGAAGAAATT GCAACTGAAA AGCTTGAAGA CGCCTCTGAT
    TCAACTGGAT TGATTCCCAG TGCTGAAAAT GTGAAAGACA CTGACGATAT CTTAGAAGTT
    GACGCAGAGG TGAATGAAGT TGAAACAGTT GAAGACATTT CAGAAGTTAC AAATGAGGAA
    ACTTCTGATG TGCCAGAAGT CATAGATGAG GAAAAGGTTG AAAAGTCTGA TGATATCTTT
    CATTTTGCAG AGGACAAACC AACTGAATTG ATTTCTGATG CTCCAGATGT AACAGAAGAC
    ATTGAAATCT CAATCTCTGC AGTTTCAGAG GATAATGAAA CAATTCAAGA AGCTGATATG
    ACAGATGTCA AAGATGAGGA AATGGAAGCT GAAAAGGTTG ATGAAATCTC CTATTTTGTA
    GAGGATAAAT CAACTGAAAT TACTTCTGAT GCTACAGATG TGAAAGAAGA CACTGATGGC
    TTTACAGTTA CAGAGGAGAA AGGAACAATT GAAAGCCTTC AAGAAGCAGC AATGAAAATG
    TCGGGAAATG CAGATGATGA AATCCCCAAT GTCACAGAGA TGGTTGAAGA ACCTGATACT
    TCTGATGTTA TGGATGAGCT AGACGAACCT GAAAAAGATG AAGATGTAAT CAGTGTCACC
    AATGAAAGGG TTACAACTGC ACAAGATGTC ATATTTTCAA AGGAAAAAAT GGGAGCAGCA
    GAACTGGATG ATCATGACAA ACTCAGGGCT AAGGATGATT CAGAAGTGGA TCACATGGAG
    AAGGAAATTG CAGATGCTGA AAATGACCTT CAGAATGAGA TAGATAAAGA TGAGTTTAAG
    GTTGAAGATG CATCTCATCA AACTATTTTG GGAAGCACTG CTGCTGAAGA AGACCAAGAT
    GATGATGATG ATGATGAAGA ACCTGTCCTT CTTACTGTCA AACATGAGCT CACAGACGGT
    GATGATGAAA ATAATAATAA CAACGACAGA AAGAGAGATG CCAGAACTAA AGGTCTGATC
    AATAGAAAAA CACCAAAGAG CCATTCCACT GAAACTGATG AACAAAAGGC GCACAAGGAT
    CTTCATAAAG AGTCTGCTAA AGTTCTTGAG CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT
    AGAGAGGAGA TATACAAAAT CATTCAAGAA ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG
    GAAGAGATCG TGTTACAACA GATAAACATA ACAGTAGAAA AGGTCAAGAA AGAAGAAACT
    GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA GAAAAGGCTC ATAAACCAAA GGATGCAAAG
    ATTGCCAAAG ACAAACCTAA AGCAGTCCCA GTAAAGCAAG AGGATGAAGT AGCCAAGAAA
    GAAACCAAAC CAGCACCATC TAAGAAAGAG GCTAAAATTG TGAAGGAGAA ACCCAAAGCA
    CATCCTGCAA AGAAAGAAAA AGCTAAAGAT ATTAAGGAAA AGGCAAAATC TGGTCAAAAA
    GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA
    ATTGATGCCA AAGTCAAACC AGCAGCAGAA AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA
    CCTAAAGTGA AGAAAGAGGC TGAAGTAATC AAAAAGATTA AACTAGAGCC TGAGAGGAAA
    GAAGCTAAAA CTCCCAAGGC CAAAATTGAA CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT
    GCTAAGGAGA AAGCAAAACC TGCTCCAGTC AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA
    GCCAAACCAG AGCCCGCAAA AAAAGAGCTT GAAGCGGTCA AAGAGAAAGC AAAACCAGCA
    CCAGCTAAAA AAGATGAAGT CAAAGAGAAA GAAAAACCAG CTCCTGTAAA GAAAGCTGAA
    GAAATTAAAA AGGCAAAAGC AGCTCCTTCA AGGAAAGAAG CTGTAAAGGA AAAACCCAAA
    CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT GCAGTTGAAA AAGCCAAGCC AAAATCTACA
    AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG AAACCTGCTC CTGAGAAGAA AGAAGCTGAA
    GTCATCATGG TGAAAACTAA ACCAGTTCTT AAAAAGAAAG AGGCTGAAGT AATCAAAGAG
    GTTAAACCAG CCCTTGAGAA GAAAGAGGCT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA GAAAAGGAAC CTGAACCAAA ACCCAAAAGA
    GAGCCAGAAA TTATTAAGGA GAAATCAGCA CCACCAAAGA AAGTTGAGGT TCCAAAACAA
    AAGGCTCCTG AAAAGAAAGC ACCTGAAATA ACCAAAGAAA AACCAAAGCC AGCACCAAAG
    AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT AAACCAGAGA AGAAAGAGGC TGAAGCCGTA
    AAGGAGAAAA CGAAACCAGC CCCTGAGAAA GAGACTGAAA TAATCAAGGA TAAAATTAAA
    GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC AAGAAGGAAG CAAAACCAGC TCCTGTAAAG
    AAAGAAACTG AAGTCATCAA AGAAAAAGAA AAACCAGCTC CTGAGAAGAA AGAGGCTGAA
    GCTGTAAAGG AGAAAACGAA ACCAACCTCT GAGAAGAAAG AGGCTGAAAT AATTAAGGAG
    AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA GTTGAAGTCA AGGAGGAAGC AAAACCAGCT
    CCTGTAAAAA AAGAAACTGA AGTCATCAAA GAAAAAGAAA AAACAGCTCC TGAGAAGAAA
    GAGGCTGAAG CTGTAAAGGA GAAAACGAAA CCAACCCCTG AGAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA TAGTTAAACA AGCTCCTGTG AAGAAAGAGG CTGAACCTGT AAAGGAGATA
    ACTAAACCAG CCCCTGAGAA GAAAGAGGCT GAAATAATCA AGGAGAAAGT TAAAGCAGCA
    CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA AAACCAGCTC CTGTAAAGAA AGAAGCTGAA
    GTCATCAAAC AAAAAGCCAA ACCAGCTCCT GAGAAGAAAG AGGCCAAAGT CATCAAGGAG
    AAAGTTAAAC CGGCTCCTGT AAAGATAGAG GCTAAACCTG TAAAGGAGAT AACTAAACCA
    GCCCCTGAGA AGAAAGAGGC TGAAATAATC AAGGAGAAGG TCAAATCAGC TCCTGTGAAG
    AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA AAGTTAAACC TGCTCCTGTG AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA
    GCTGAAGCAG TTTATGAGAA AAAAGAGGCT GAAGTAGCCA AGGAGATAGT TAAACCAACT
    CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG GAAAAGGCTC CTGAATCAAA ACCCCAAAAA
    GAGGCTGAAA TTATCAAAGA GAAAGTTAAA CCTGCACCTG TAAAGAAAGA GCCTGAAGTT
    GCAAAAGTAC CAAAACCCAA AAAAGAGGCT GAACTCGCCA AAGAGAAAGT TAAACCTGCA
    CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA GCACCAAAGA AAGAGGCTGA AGCTATTAAG
    GAGAAACGTA CACCAGCTCC TGAAAAGAAA GTGGTAGAAG TCATCAAGGA GAAAGTCAAA
    CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG GAGAAAGTTA GACCTGCTCC TGAAAAGAAA
    GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT GAACCAAAAC CCCAAAAAGA GGTTGAAGTC
    ATCAAAGAGA AAGCTAAACC AGCCCTTAAG AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT
    CAACCAGCTC CTATAAAGAA AGAGCCAGAG CTTCCCAAAG AAAAGGTGCC TGAACCAACA
    CCAGAACCTG AGAGAGTTGA AGCTCCAAAA GAAAAGGCAA AAGTAATCCC TAGGGAAAAA
    GTAGCTGAGA TTACTAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGTAAAG
    GAGAAAGCTA CACCTGCTCC TGTGCACAAA GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA
    CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCT
    AAAAAGACTG AAGTCATCAA AGCGAAAGTA AAACCAGCCC CTGAGAAAAA AGAGGCTGAA
    GTGAAGGAGA AAATTAAACC AGTCCCTGAG AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA
    ATTAAACCTG CTCCTGTAAA GAAAGAGCCT GAAGTTCCTA AAGAAAAGGC TCCTGAACCA
    AAACCCAAAA AAGAACCTGA AGTTATCAAA GAGAAAGTGA AACCAGCGCC AGAGAAGAAA
    GCACCCGAAA TAACCAAAGA AAAACCAAAG CCAGCAGCAA AGAAAGTAGC TGAAGTCATC
    AAAGACAGAA CTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGAAACCA
    AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA GTCATTGAAA AGAAAGTTAT ACCAGCTCCC
    GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA AAGGCTCCTG AACTGACAAC AAAAAAAGCA
    CCTGAGATAA CCAAAGAAAA ACCGAAGCCA GTACCAAAGA AAGAGGCTGA AGTCATTAAA
    GAGAAAGCTA AACTAGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAGGA GAAAGTTAAA
    CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT CATAAGGAAA AAGCACCTGA ACCAAAATCA
    GAACCTACAA AAAAAGTTGA GGTTCCAAAG GAAAAGGCAA AACCAGTTGC TAAAGAAAAG
    GAACCTGAAA TAACCAAAGA AAAGCCAAAA CCAGCACCAA AGAAAGAAGC TGTAGTCATC
    AAAGAAAAAG CTGTACCAGC CATCCTGAAG GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA
    GCTCCTGAGA AGAAAGTGGC TGAAATTAAG GAGAAAGTTA AACCAGCTTC TATAATGAAA
    GAGCCTGAAG TTGTGAAACA AAAGACTCCT GAACCGAAAC CCAAAAAAGA GCCTGAAGTT
    GTGAAAGAAA AGGCTCCTGA ACCTAAACCC AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA
    GCCAAACCAG CACCTCAGAA GAAAGAAGTC ATCAAAGAAA AAGTCAAACT TGCTCCTGTA
    AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG GCACTTAAAC CAGAACCCAA AAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149673
    Clone ID Related Accession (Same CDS sequence) XM_021467337.1
    Accession Version XM_021467337.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGCTGA AGTCATCAAA
    CAAAAAGCCA AACCAGCTCC TGAGAAGAAA GAGGCCAAAG TCATCAAGGA GAAAGTTAAA
    CCGGCTCCTG TAAAGATAGA GGCTAAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAG GTCAAATCAG CTCCTGTGAA GAAAGAAGTT
    GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT GAAAAGAAAG AGGCTGAAGT CATCAAGGAG
    AAAGTTAAAC CTGCTCCTGT GAGGAAAGAA GCTGAAGAAA TAAAGGAAAA AGCTGAAGCA
    GTTTATGAGA AAAAAGAGGC TGAAGTAGCC AAGGAGATAG TTAAACCAAC TCCTTTAAAG
    AAGGAGCCCG AAGTTGTGAA GGAAAAGGCT CCTGAATCAA AACCCCAAAA AGAGGCTGAA
    ATTATCAAAG AGAAAGTTAA ACCTGCACCT GTAAAGAAAG AGCCTGAAGT TGCAAAAGTA
    CCAAAACCCA AAAAAGAGGC TGAACTCGCC AAAGAGAAAG TTAAACCTGC ACCTGTAAAG
    AAAGAAGAAA AACCTAAGGC AGCACCAAAG AAAGAGGCTG AAGCTATTAA GGAGAAACGT
    ACACCAGCTC CTGAAAAGAA AGTGGTAGAA GTCATCAAGG AGAAAGTCAA ACCAGCCCCT
    GAGAAAAAAG CGGTTCTCAA GGAGAAAGTT AGACCTGCTC CTGAAAAGAA AGAGCCTGAA
    GTTGCTAAAG AAAAGGCTCC TGAACCAAAA CCCCAAAAAG AGGTTGAAGT CATCAAAGAG
    AAAGCTAAAC CAGCCCTTAA GAAGAAAGAG CCTAAAGTCA AGGAAAAGGT TCAACCAGCT
    CCTATAAAGA AAGAGCCAGA GCTTCCCAAA GAAAAGGTGC CTGAACCAAC ACCAGAACCT
    GAGAGAGTTG AAGCTCCAAA AGAAAAGGCA AAAGTAATCC CTAGGGAAAA AGTAGCTGAG
    ATTACTAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGTAAA GGAGAAAGCT
    ACACCTGCTC CTGTGCACAA AGAGGTTGAA GTGTTCAAGG AGAAAGTTAA ACAAGCTCCT
    GTAAAGAAAG AGCCAGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC TAAAAAGACT
    GAAGTCATCA AAGCGAAAGT AAAACCAGCC CCTGAGAAAA AAGAGGCTGA AGTGAAGGAG
    AAAATTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TAAAGGAGAG AATTAAACCT
    GCTCCTGTAA AGAAAGAGCC TGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCCAAA
    AAAGAACCTG AAGTTATCAA AGAGAAAGTG AAACCAGCGC CAGAGAAGAA AGCACCCGAA
    ATAACCAAAG AAAAACCAAA GCCAGCAGCA AAGAAAGTAG CTGAAGTCAT CAAAGACAGA
    ACTAAACCAG TCCCTGAGAA GAAAGAGGCT GAAGTCATCA AGGAGAAACC AAAAGCAGTT
    CCCGAGAAGA AAGAGGCTAA AGTCATTGAA AAGAAAGTTA TACCAGCTCC CGTAAAGAAA
    GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT GAACTGACAA CAAAAAAAGC ACCTGAGATA
    ACCAAAGAAA AACCGAAGCC AGTACCAAAG AAAGAGGCTG AAGTCATTAA AGAGAAAGCT
    AAACTAGCTC CTGAAAAGAA AGAGGTTGAA GTCATCAAGG AGAAAGTTAA ACCAGCTCCG
    TTAAAGAAAG AGCCTGAGGT TCATAAGGAA AAAGCACCTG AACCAAAATC AGAACCTACA
    AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA AAACCAGTTG CTAAAGAAAA GGAACCTGAA
    ATAACCAAAG AAAAGCCAAA ACCAGCACCA AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA
    GCTGTACCAG CCATCCTGAA GGAAGTGGCT GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG
    AAGAAAGTGG CTGAAATTAA GGAGAAAGTT AAACCAGCTT CTATAATGAA AGAGCCTGAA
    GTTGTGAAAC AAAAGACTCC TGAACCGAAA CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA
    AAGGCTCCTG AACCTAAACC CAAAAAAGAG CCTGAAGTTG CTAAAGAGAA AGCCAAACCA
    GCACCTCAGA AGAAAGAAGT CATCAAAGAA AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG
    CCCGAAGTTG TCGAAGAAAA GGCACTTAAA CCAGAACCCA AAAAAGATGA AGTTGGTCTT
    ATTGGTCTGG ACCTGGAGCT CCTAAACTCC ATCAACCAAA AACTGTCTCT CCTGGACTTG
    CTAAGGAAGG ACATTGGAGA AATGAAAAGC GATCTGGAGT CCACTCAGAA TCAAATCACA
    CTTTTACGGA TGGACAACAG AACGATCAAA GAGCCAGAGA CTATTGGTGT GAAAGTAAAA
    CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC CACGCTCTGA TCAATGCTAC TAGGACTGCA
    CATATTAAAA CAGAGCGTGA ATCTCTAAAA AACATCACGA AACCAGTGCC TGCAAAGAAA
    GCCAAAGAGG CTCCAAAGGT TAAGATTGCG CTGGAGCCTA AAAAGAAAGG AATCATCCCT
    GAGAAGGTCA AAGAAGTAAA GGAAACTTTA CAGAAAGAAC CTGAGTTGGT CAAAGATAAA
    GTAGTTGAGC CAATAGAGAA GGAAATTGTT GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT
    GAAATCACCA AAGATATTCC AGAGGTGGAG GAAGAGGACA TTCCCTACTT CCAGTGTTTC
    TTTGTGGATG AGGATGACAC TCAGTATCCG TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323012.1
    CDS338..8377
    Translation

    Target ORF information:

    RefSeq Version XM_021467337.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGCTGA AGTCATCAAA
    CAAAAAGCCA AACCAGCTCC TGAGAAGAAA GAGGCCAAAG TCATCAAGGA GAAAGTTAAA
    CCGGCTCCTG TAAAGATAGA GGCTAAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAG GTCAAATCAG CTCCTGTGAA GAAAGAAGTT
    GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT GAAAAGAAAG AGGCTGAAGT CATCAAGGAG
    AAAGTTAAAC CTGCTCCTGT GAGGAAAGAA GCTGAAGAAA TAAAGGAAAA AGCTGAAGCA
    GTTTATGAGA AAAAAGAGGC TGAAGTAGCC AAGGAGATAG TTAAACCAAC TCCTTTAAAG
    AAGGAGCCCG AAGTTGTGAA GGAAAAGGCT CCTGAATCAA AACCCCAAAA AGAGGCTGAA
    ATTATCAAAG AGAAAGTTAA ACCTGCACCT GTAAAGAAAG AGCCTGAAGT TGCAAAAGTA
    CCAAAACCCA AAAAAGAGGC TGAACTCGCC AAAGAGAAAG TTAAACCTGC ACCTGTAAAG
    AAAGAAGAAA AACCTAAGGC AGCACCAAAG AAAGAGGCTG AAGCTATTAA GGAGAAACGT
    ACACCAGCTC CTGAAAAGAA AGTGGTAGAA GTCATCAAGG AGAAAGTCAA ACCAGCCCCT
    GAGAAAAAAG CGGTTCTCAA GGAGAAAGTT AGACCTGCTC CTGAAAAGAA AGAGCCTGAA
    GTTGCTAAAG AAAAGGCTCC TGAACCAAAA CCCCAAAAAG AGGTTGAAGT CATCAAAGAG
    AAAGCTAAAC CAGCCCTTAA GAAGAAAGAG CCTAAAGTCA AGGAAAAGGT TCAACCAGCT
    CCTATAAAGA AAGAGCCAGA GCTTCCCAAA GAAAAGGTGC CTGAACCAAC ACCAGAACCT
    GAGAGAGTTG AAGCTCCAAA AGAAAAGGCA AAAGTAATCC CTAGGGAAAA AGTAGCTGAG
    ATTACTAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGTAAA GGAGAAAGCT
    ACACCTGCTC CTGTGCACAA AGAGGTTGAA GTGTTCAAGG AGAAAGTTAA ACAAGCTCCT
    GTAAAGAAAG AGCCAGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC TAAAAAGACT
    GAAGTCATCA AAGCGAAAGT AAAACCAGCC CCTGAGAAAA AAGAGGCTGA AGTGAAGGAG
    AAAATTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TAAAGGAGAG AATTAAACCT
    GCTCCTGTAA AGAAAGAGCC TGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCCAAA
    AAAGAACCTG AAGTTATCAA AGAGAAAGTG AAACCAGCGC CAGAGAAGAA AGCACCCGAA
    ATAACCAAAG AAAAACCAAA GCCAGCAGCA AAGAAAGTAG CTGAAGTCAT CAAAGACAGA
    ACTAAACCAG TCCCTGAGAA GAAAGAGGCT GAAGTCATCA AGGAGAAACC AAAAGCAGTT
    CCCGAGAAGA AAGAGGCTAA AGTCATTGAA AAGAAAGTTA TACCAGCTCC CGTAAAGAAA
    GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT GAACTGACAA CAAAAAAAGC ACCTGAGATA
    ACCAAAGAAA AACCGAAGCC AGTACCAAAG AAAGAGGCTG AAGTCATTAA AGAGAAAGCT
    AAACTAGCTC CTGAAAAGAA AGAGGTTGAA GTCATCAAGG AGAAAGTTAA ACCAGCTCCG
    TTAAAGAAAG AGCCTGAGGT TCATAAGGAA AAAGCACCTG AACCAAAATC AGAACCTACA
    AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA AAACCAGTTG CTAAAGAAAA GGAACCTGAA
    ATAACCAAAG AAAAGCCAAA ACCAGCACCA AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA
    GCTGTACCAG CCATCCTGAA GGAAGTGGCT GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG
    AAGAAAGTGG CTGAAATTAA GGAGAAAGTT AAACCAGCTT CTATAATGAA AGAGCCTGAA
    GTTGTGAAAC AAAAGACTCC TGAACCGAAA CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA
    AAGGCTCCTG AACCTAAACC CAAAAAAGAG CCTGAAGTTG CTAAAGAGAA AGCCAAACCA
    GCACCTCAGA AGAAAGAAGT CATCAAAGAA AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG
    CCCGAAGTTG TCGAAGAAAA GGCACTTAAA CCAGAACCCA AAAAAGATGA AGTTGGTCTT
    ATTGGTCTGG ACCTGGAGCT CCTAAACTCC ATCAACCAAA AACTGTCTCT CCTGGACTTG
    CTAAGGAAGG ACATTGGAGA AATGAAAAGC GATCTGGAGT CCACTCAGAA TCAAATCACA
    CTTTTACGGA TGGACAACAG AACGATCAAA GAGCCAGAGA CTATTGGTGT GAAAGTAAAA
    CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC CACGCTCTGA TCAATGCTAC TAGGACTGCA
    CATATTAAAA CAGAGCGTGA ATCTCTAAAA AACATCACGA AACCAGTGCC TGCAAAGAAA
    GCCAAAGAGG CTCCAAAGGT TAAGATTGCG CTGGAGCCTA AAAAGAAAGG AATCATCCCT
    GAGAAGGTCA AAGAAGTAAA GGAAACTTTA CAGAAAGAAC CTGAGTTGGT CAAAGATAAA
    GTAGTTGAGC CAATAGAGAA GGAAATTGTT GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT
    GAAATCACCA AAGATATTCC AGAGGTGGAG GAAGAGGACA TTCCCTACTT CCAGTGTTTC
    TTTGTGGATG AGGATGACAC TCAGTATCCG TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149674
    Clone ID Related Accession (Same CDS sequence) XM_021467338.1
    Accession Version XM_021467338.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAATTAAGGA GAAAGTTAAA CCAGCTTCTA TAATGAAAGA GCCTGAAGTT
    GTGAAACAAA AGACTCCTGA ACCGAAACCC AAAAAAGAGC CTGAAGTTGT GAAAGAAAAG
    GCTCCTGAAC CTAAACCCAA AAAAGAGCCT GAAGTTGCTA AAGAGAAAGC CAAACCAGCA
    CCTCAGAAGA AAGAAGTCAT CAAAGAAAAA GTCAAACTTG CTCCTGTAAA GAAAGTGCCC
    GAAGTTGTCG AAGAAAAGGC ACTTAAACCA GAACCCAAAA AAGATGAAGT TGGTCTTATT
    GGTCTGGACC TGGAGCTCCT AAACTCCATC AACCAAAAAC TGTCTCTCCT GGACTTGCTA
    AGGAAGGACA TTGGAGAAAT GAAAAGCGAT CTGGAGTCCA CTCAGAATCA AATCACACTT
    TTACGGATGG ACAACAGAAC GATCAAAGAG CCAGAGACTA TTGGTGTGAA AGTAAAACCA
    GCAGTCAAGG AAAAAGCAAA AAGAGACCAC GCTCTGATCA ATGCTACTAG GACTGCACAT
    ATTAAAACAG AGCGTGAATC TCTAAAAAAC ATCACGAAAC CAGTGCCTGC AAAGAAAGCC
    AAAGAGGCTC CAAAGGTTAA GATTGCGCTG GAGCCTAAAA AGAAAGGAAT CATCCCTGAG
    AAGGTCAAAG AAGTAAAGGA AACTTTACAG AAAGAACCTG AGTTGGTCAA AGATAAAGTA
    GTTGAGCCAA TAGAGAAGGA AATTGTTGTT GAGAAGGAAG TCAAAGGAGA AGAGCCTGAA
    ATCACCAAAG ATATTCCAGA GGTGGAGGAA GAGGACATTC CCTACTTCCA GTGTTTCTTT
    GTGGATGAGG ATGACACTCA GTATCCGTTC TTCCCTTTCT CTCCACCGCT TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323013.1
    CDS338..8374
    Translation

    Target ORF information:

    RefSeq Version XM_021467338.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467338.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAATTAAGGA GAAAGTTAAA CCAGCTTCTA TAATGAAAGA GCCTGAAGTT
    GTGAAACAAA AGACTCCTGA ACCGAAACCC AAAAAAGAGC CTGAAGTTGT GAAAGAAAAG
    GCTCCTGAAC CTAAACCCAA AAAAGAGCCT GAAGTTGCTA AAGAGAAAGC CAAACCAGCA
    CCTCAGAAGA AAGAAGTCAT CAAAGAAAAA GTCAAACTTG CTCCTGTAAA GAAAGTGCCC
    GAAGTTGTCG AAGAAAAGGC ACTTAAACCA GAACCCAAAA AAGATGAAGT TGGTCTTATT
    GGTCTGGACC TGGAGCTCCT AAACTCCATC AACCAAAAAC TGTCTCTCCT GGACTTGCTA
    AGGAAGGACA TTGGAGAAAT GAAAAGCGAT CTGGAGTCCA CTCAGAATCA AATCACACTT
    TTACGGATGG ACAACAGAAC GATCAAAGAG CCAGAGACTA TTGGTGTGAA AGTAAAACCA
    GCAGTCAAGG AAAAAGCAAA AAGAGACCAC GCTCTGATCA ATGCTACTAG GACTGCACAT
    ATTAAAACAG AGCGTGAATC TCTAAAAAAC ATCACGAAAC CAGTGCCTGC AAAGAAAGCC
    AAAGAGGCTC CAAAGGTTAA GATTGCGCTG GAGCCTAAAA AGAAAGGAAT CATCCCTGAG
    AAGGTCAAAG AAGTAAAGGA AACTTTACAG AAAGAACCTG AGTTGGTCAA AGATAAAGTA
    GTTGAGCCAA TAGAGAAGGA AATTGTTGTT GAGAAGGAAG TCAAAGGAGA AGAGCCTGAA
    ATCACCAAAG ATATTCCAGA GGTGGAGGAA GAGGACATTC CCTACTTCCA GTGTTTCTTT
    GTGGATGAGG ATGACACTCA GTATCCGTTC TTCCCTTTCT CTCCACCGCT TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149675
    Clone ID Related Accession (Same CDS sequence) XM_021467339.1
    Accession Version XM_021467339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAAGC TAAAACTCCC
    AAGGCCAAAA TTGAACCAGC TGGTTTGAAG AAAGATGCTG AAGCTGCTAA GGAGAAAGCA
    AAACCTGCTC CAGTCAAGAA AGGGCCTGAG GTTTCCAGAG AAAAAGCCAA ACCAGAGCCC
    GCAAAAAAAG AGCTTGAAGC GGTCAAAGAG AAAGCAAAAC CAGCACCAGC TAAAAAAGAT
    GAAGTCAAAG AGAAAGAAAA ACCAGCTCCT GTAAAGAAAG CTGAAGAAAT TAAAAAGGCA
    AAAGCAGCTC CTTCAAGGAA AGAAGCTGTA AAGGAAAAAC CCAAACCTGT TTCCGAAAAG
    AAAGAACCTG CTGTTGCAGT TGAAAAAGCC AAGCCAAAAT CTACAAAGAA AGAAGCTGAA
    GTCGAGGAAA AAGTGAAACC TGCTCCTGAG AAGAAAGAAG CTGAAGTCAT CATGGTGAAA
    ACTAAACCAG TTCTTAAAAA GAAAGAGGCT GAAGTAATCA AAGAGGTTAA ACCAGCCCTT
    GAGAAGAAAG AGGCTGAAGT CATCAAGGAG AAAGTTAAAC CAGCTCCTGT AAAGAAAGAG
    CCTGAAGTTG TTAAAGAAAA GGAACCTGAA CCAAAACCCA AAAGAGAGCC AGAAATTATT
    AAGGAGAAAT CAGCACCACC AAAGAAAGTT GAGGTTCCAA AACAAAAGGC TCCTGAAAAG
    AAAGCACCTG AAATAACCAA AGAAAAACCA AAGCCAGCAC CAAAGAAAGA GGCTGAAGCT
    GTAAAAGAGA AAGCTAAACC AGAGAAGAAA GAGGCTGAAG CCGTAAAGGA GAAAACGAAA
    CCAGCCCCTG AGAAAGAGAC TGAAATAATC AAGGATAAAA TTAAAGCAGC TCCTGTGAAG
    AAAGAAGTTG AAGTCAAGAA GGAAGCAAAA CCAGCTCCTG TAAAGAAAGA AACTGAAGTC
    ATCAAAGAAA AAGAAAAACC AGCTCCTGAG AAGAAAGAGG CTGAAGCTGT AAAGGAGAAA
    ACGAAACCAA CCTCTGAGAA GAAAGAGGCT GAAATAATTA AGGAGAAAGT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGT AAAAAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAAACA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGATAGTT
    AAACAAGCTC CTGTGAAGAA AGAGGCTGAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAAGTTAAAG CAGCACCTCT GAAGAAAGAA
    GTCAAGGAGA AAGCAAAACC AGCTCCTGTA AAGAAAGAAG CTGAAGTCAT CAAACAAAAA
    GCCAAACCAG CTCCTGAGAA GAAAGAGGCC AAAGTCATCA AGGAGAAAGT TAAACCGGCT
    CCTGTAAAGA TAGAGGCTAA ACCTGTAAAG GAGATAACTA AACCAGCCCC TGAGAAGAAA
    GAGGCTGAAA TAATCAAGGA GAAGGTCAAA TCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGGAGGAAG CAAAACCAGC TCCTGAAAAG AAAGAGGCTG AAGTCATCAA GGAGAAAGTT
    AAACCTGCTC CTGTGAGGAA AGAAGCTGAA GAAATAAAGG AAAAAGCTGA AGCAGTTTAT
    GAGAAAAAAG AGGCTGAAGT AGCCAAGGAG ATAGTTAAAC CAACTCCTTT AAAGAAGGAG
    CCCGAAGTTG TGAAGGAAAA GGCTCCTGAA TCAAAACCCC AAAAAGAGGC TGAAATTATC
    AAAGAGAAAG TTAAACCTGC ACCTGTAAAG AAAGAGCCTG AAGTTGCAAA AGTACCAAAA
    CCCAAAAAAG AGGCTGAACT CGCCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAA
    GAAAAACCTA AGGCAGCACC AAAGAAAGAG GCTGAAGCTA TTAAGGAGAA ACGTACACCA
    GCTCCTGAAA AGAAAGTGGT AGAAGTCATC AAGGAGAAAG TCAAACCAGC CCCTGAGAAA
    AAAGCGGTTC TCAAGGAGAA AGTTAGACCT GCTCCTGAAA AGAAAGAGCC TGAAGTTGCT
    AAAGAAAAGG CTCCTGAACC AAAACCCCAA AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323014.1
    CDS338..8371
    Translation

    Target ORF information:

    RefSeq Version XM_021467339.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAAGC TAAAACTCCC
    AAGGCCAAAA TTGAACCAGC TGGTTTGAAG AAAGATGCTG AAGCTGCTAA GGAGAAAGCA
    AAACCTGCTC CAGTCAAGAA AGGGCCTGAG GTTTCCAGAG AAAAAGCCAA ACCAGAGCCC
    GCAAAAAAAG AGCTTGAAGC GGTCAAAGAG AAAGCAAAAC CAGCACCAGC TAAAAAAGAT
    GAAGTCAAAG AGAAAGAAAA ACCAGCTCCT GTAAAGAAAG CTGAAGAAAT TAAAAAGGCA
    AAAGCAGCTC CTTCAAGGAA AGAAGCTGTA AAGGAAAAAC CCAAACCTGT TTCCGAAAAG
    AAAGAACCTG CTGTTGCAGT TGAAAAAGCC AAGCCAAAAT CTACAAAGAA AGAAGCTGAA
    GTCGAGGAAA AAGTGAAACC TGCTCCTGAG AAGAAAGAAG CTGAAGTCAT CATGGTGAAA
    ACTAAACCAG TTCTTAAAAA GAAAGAGGCT GAAGTAATCA AAGAGGTTAA ACCAGCCCTT
    GAGAAGAAAG AGGCTGAAGT CATCAAGGAG AAAGTTAAAC CAGCTCCTGT AAAGAAAGAG
    CCTGAAGTTG TTAAAGAAAA GGAACCTGAA CCAAAACCCA AAAGAGAGCC AGAAATTATT
    AAGGAGAAAT CAGCACCACC AAAGAAAGTT GAGGTTCCAA AACAAAAGGC TCCTGAAAAG
    AAAGCACCTG AAATAACCAA AGAAAAACCA AAGCCAGCAC CAAAGAAAGA GGCTGAAGCT
    GTAAAAGAGA AAGCTAAACC AGAGAAGAAA GAGGCTGAAG CCGTAAAGGA GAAAACGAAA
    CCAGCCCCTG AGAAAGAGAC TGAAATAATC AAGGATAAAA TTAAAGCAGC TCCTGTGAAG
    AAAGAAGTTG AAGTCAAGAA GGAAGCAAAA CCAGCTCCTG TAAAGAAAGA AACTGAAGTC
    ATCAAAGAAA AAGAAAAACC AGCTCCTGAG AAGAAAGAGG CTGAAGCTGT AAAGGAGAAA
    ACGAAACCAA CCTCTGAGAA GAAAGAGGCT GAAATAATTA AGGAGAAAGT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGT AAAAAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAAACA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGATAGTT
    AAACAAGCTC CTGTGAAGAA AGAGGCTGAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAAGTTAAAG CAGCACCTCT GAAGAAAGAA
    GTCAAGGAGA AAGCAAAACC AGCTCCTGTA AAGAAAGAAG CTGAAGTCAT CAAACAAAAA
    GCCAAACCAG CTCCTGAGAA GAAAGAGGCC AAAGTCATCA AGGAGAAAGT TAAACCGGCT
    CCTGTAAAGA TAGAGGCTAA ACCTGTAAAG GAGATAACTA AACCAGCCCC TGAGAAGAAA
    GAGGCTGAAA TAATCAAGGA GAAGGTCAAA TCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGGAGGAAG CAAAACCAGC TCCTGAAAAG AAAGAGGCTG AAGTCATCAA GGAGAAAGTT
    AAACCTGCTC CTGTGAGGAA AGAAGCTGAA GAAATAAAGG AAAAAGCTGA AGCAGTTTAT
    GAGAAAAAAG AGGCTGAAGT AGCCAAGGAG ATAGTTAAAC CAACTCCTTT AAAGAAGGAG
    CCCGAAGTTG TGAAGGAAAA GGCTCCTGAA TCAAAACCCC AAAAAGAGGC TGAAATTATC
    AAAGAGAAAG TTAAACCTGC ACCTGTAAAG AAAGAGCCTG AAGTTGCAAA AGTACCAAAA
    CCCAAAAAAG AGGCTGAACT CGCCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAA
    GAAAAACCTA AGGCAGCACC AAAGAAAGAG GCTGAAGCTA TTAAGGAGAA ACGTACACCA
    GCTCCTGAAA AGAAAGTGGT AGAAGTCATC AAGGAGAAAG TCAAACCAGC CCCTGAGAAA
    AAAGCGGTTC TCAAGGAGAA AGTTAGACCT GCTCCTGAAA AGAAAGAGCC TGAAGTTGCT
    AAAGAAAAGG CTCCTGAACC AAAACCCCAA AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149676
    Clone ID Related Accession (Same CDS sequence) XM_021467340.1
    Accession Version XM_021467340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323015.1
    CDS338..8371
    Translation

    Target ORF information:

    RefSeq Version XM_021467340.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149677
    Clone ID Related Accession (Same CDS sequence) XM_021467341.1
    Accession Version XM_021467341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AACTGAAGTC
    ATCAAAGAAA AAGAAAAACC AGCTCCTGAG AAGAAAGAGG CTGAAGCTGT AAAGGAGAAA
    ACGAAACCAA CCTCTGAGAA GAAAGAGGCT GAAATAATTA AGGAGAAAGT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGT AAAAAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAAACA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGATAGTT
    AAACAAGCTC CTGTGAAGAA AGAGGCTGAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAAGTTAAAG CAGCACCTCT GAAGAAAGAA
    GTCAAGGAGA AAGCAAAACC AGCTCCTGTA AAGAAAGAAG CTGAAGTCAT CAAACAAAAA
    GCCAAACCAG CTCCTGAGAA GAAAGAGGCC AAAGTCATCA AGGAGAAAGT TAAACCGGCT
    CCTGTAAAGA TAGAGGCTAA ACCTGTAAAG GAGATAACTA AACCAGCCCC TGAGAAGAAA
    GAGGCTGAAA TAATCAAGGA GAAGGTCAAA TCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGGAGGAAG CAAAACCAGC TCCTGAAAAG AAAGAGGCTG AAGTCATCAA GGAGAAAGTT
    AAACCTGCTC CTGTGAGGAA AGAAGCTGAA GAAATAAAGG AAAAAGCTGA AGCAGTTTAT
    GAGAAAAAAG AGGCTGAAGT AGCCAAGGAG ATAGTTAAAC CAACTCCTTT AAAGAAGGAG
    CCCGAAGTTG TGAAGGAAAA GGCTCCTGAA TCAAAACCCC AAAAAGAGGC TGAAATTATC
    AAAGAGAAAG TTAAACCTGC ACCTGTAAAG AAAGAGCCTG AAGTTGCAAA AGTACCAAAA
    CCCAAAAAAG AGGCTGAACT CGCCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAA
    GAAAAACCTA AGGCAGCACC AAAGAAAGAG GCTGAAGCTA TTAAGGAGAA ACGTACACCA
    GCTCCTGAAA AGAAAGTGGT AGAAGTCATC AAGGAGAAAG TCAAACCAGC CCCTGAGAAA
    AAAGCGGTTC TCAAGGAGAA AGTTAGACCT GCTCCTGAAA AGAAAGAGCC TGAAGTTGCT
    AAAGAAAAGG CTCCTGAACC AAAACCCCAA AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323016.1
    CDS338..8371
    Translation

    Target ORF information:

    RefSeq Version XM_021467341.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AACTGAAGTC
    ATCAAAGAAA AAGAAAAACC AGCTCCTGAG AAGAAAGAGG CTGAAGCTGT AAAGGAGAAA
    ACGAAACCAA CCTCTGAGAA GAAAGAGGCT GAAATAATTA AGGAGAAAGT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGT AAAAAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAAACA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGATAGTT
    AAACAAGCTC CTGTGAAGAA AGAGGCTGAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAAGTTAAAG CAGCACCTCT GAAGAAAGAA
    GTCAAGGAGA AAGCAAAACC AGCTCCTGTA AAGAAAGAAG CTGAAGTCAT CAAACAAAAA
    GCCAAACCAG CTCCTGAGAA GAAAGAGGCC AAAGTCATCA AGGAGAAAGT TAAACCGGCT
    CCTGTAAAGA TAGAGGCTAA ACCTGTAAAG GAGATAACTA AACCAGCCCC TGAGAAGAAA
    GAGGCTGAAA TAATCAAGGA GAAGGTCAAA TCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGGAGGAAG CAAAACCAGC TCCTGAAAAG AAAGAGGCTG AAGTCATCAA GGAGAAAGTT
    AAACCTGCTC CTGTGAGGAA AGAAGCTGAA GAAATAAAGG AAAAAGCTGA AGCAGTTTAT
    GAGAAAAAAG AGGCTGAAGT AGCCAAGGAG ATAGTTAAAC CAACTCCTTT AAAGAAGGAG
    CCCGAAGTTG TGAAGGAAAA GGCTCCTGAA TCAAAACCCC AAAAAGAGGC TGAAATTATC
    AAAGAGAAAG TTAAACCTGC ACCTGTAAAG AAAGAGCCTG AAGTTGCAAA AGTACCAAAA
    CCCAAAAAAG AGGCTGAACT CGCCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAA
    GAAAAACCTA AGGCAGCACC AAAGAAAGAG GCTGAAGCTA TTAAGGAGAA ACGTACACCA
    GCTCCTGAAA AGAAAGTGGT AGAAGTCATC AAGGAGAAAG TCAAACCAGC CCCTGAGAAA
    AAAGCGGTTC TCAAGGAGAA AGTTAGACCT GCTCCTGAAA AGAAAGAGCC TGAAGTTGCT
    AAAGAAAAGG CTCCTGAACC AAAACCCCAA AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149678
    Clone ID Related Accession (Same CDS sequence) XM_021467342.1
    Accession Version XM_021467342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323017.1
    CDS338..8371
    Translation

    Target ORF information:

    RefSeq Version XM_021467342.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149679
    Clone ID Related Accession (Same CDS sequence) XM_021467343.1
    Accession Version XM_021467343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323018.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467343.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149680
    Clone ID Related Accession (Same CDS sequence) XM_021467344.1
    Accession Version XM_021467344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGCTAA AACTCCCAAG
    GCCAAAATTG AACCAGCTGG TTTGAAGAAA GATGCTGAAG CTGCTAAGGA GAAAGCAAAA
    CCTGCTCCAG TCAAGAAAGG GCCTGAGGTT TCCAGAGAAA AAGCCAAACC AGAGCCCGCA
    AAAAAAGAGC TTGAAGCGGT CAAAGAGAAA GCAAAACCAG CACCAGCTAA AAAAGATGAA
    GTCAAAGAGA AAGAAAAACC AGCTCCTGTA AAGAAAGCTG AAGAAATTAA AAAGGCAAAA
    GCAGCTCCTT CAAGGAAAGA AGCTGTAAAG GAAAAACCCA AACCTGTTTC CGAAAAGAAA
    GAACCTGCTG TTGCAGTTGA AAAAGCCAAG CCAAAATCTA CAAAGAAAGA AGCTGAAGTC
    GAGGAAAAAG TGAAACCTGC TCCTGAGAAG AAAGAAGCTG AAGTCATCAT GGTGAAAACT
    AAACCAGTTC TTAAAAAGAA AGAGGCTGAA GTAATCAAAG AGGTTAAACC AGCCCTTGAG
    AAGAAAGAGG CTGAAGTCAT CAAGGAGAAA GTTAAACCAG CTCCTGTAAA GAAAGAGCCT
    GAAGTTGTTA AAGAAAAGGA ACCTGAACCA AAACCCAAAA GAGAGCCAGA AATTATTAAG
    GAGAAATCAG CACCACCAAA GAAAGTTGAG GTTCCAAAAC AAAAGGCTCC TGAAAAGAAA
    GCACCTGAAA TAACCAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGCTGTA
    AAAGAGAAAG CTAAACCAGA GAAGAAAGAG GCTGAAGCCG TAAAGGAGAA AACGAAACCA
    GCCCCTGAGA AAGAGACTGA AATAATCAAG GATAAAATTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGAAGGA AGCAAAACCA GCTCCTGTAA AGAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAACCAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCT CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323019.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467344.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGCTAA AACTCCCAAG
    GCCAAAATTG AACCAGCTGG TTTGAAGAAA GATGCTGAAG CTGCTAAGGA GAAAGCAAAA
    CCTGCTCCAG TCAAGAAAGG GCCTGAGGTT TCCAGAGAAA AAGCCAAACC AGAGCCCGCA
    AAAAAAGAGC TTGAAGCGGT CAAAGAGAAA GCAAAACCAG CACCAGCTAA AAAAGATGAA
    GTCAAAGAGA AAGAAAAACC AGCTCCTGTA AAGAAAGCTG AAGAAATTAA AAAGGCAAAA
    GCAGCTCCTT CAAGGAAAGA AGCTGTAAAG GAAAAACCCA AACCTGTTTC CGAAAAGAAA
    GAACCTGCTG TTGCAGTTGA AAAAGCCAAG CCAAAATCTA CAAAGAAAGA AGCTGAAGTC
    GAGGAAAAAG TGAAACCTGC TCCTGAGAAG AAAGAAGCTG AAGTCATCAT GGTGAAAACT
    AAACCAGTTC TTAAAAAGAA AGAGGCTGAA GTAATCAAAG AGGTTAAACC AGCCCTTGAG
    AAGAAAGAGG CTGAAGTCAT CAAGGAGAAA GTTAAACCAG CTCCTGTAAA GAAAGAGCCT
    GAAGTTGTTA AAGAAAAGGA ACCTGAACCA AAACCCAAAA GAGAGCCAGA AATTATTAAG
    GAGAAATCAG CACCACCAAA GAAAGTTGAG GTTCCAAAAC AAAAGGCTCC TGAAAAGAAA
    GCACCTGAAA TAACCAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGCTGTA
    AAAGAGAAAG CTAAACCAGA GAAGAAAGAG GCTGAAGCCG TAAAGGAGAA AACGAAACCA
    GCCCCTGAGA AAGAGACTGA AATAATCAAG GATAAAATTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGAAGGA AGCAAAACCA GCTCCTGTAA AGAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAACCAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCT CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149681
    Clone ID Related Accession (Same CDS sequence) XM_021467345.1
    Accession Version XM_021467345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCAGA AATTATTAAG
    GAGAAATCAG CACCACCAAA GAAAGTTGAG GTTCCAAAAC AAAAGGCTCC TGAAAAGAAA
    GCACCTGAAA TAACCAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGCTGTA
    AAAGAGAAAG CTAAACCAGA GAAGAAAGAG GCTGAAGCCG TAAAGGAGAA AACGAAACCA
    GCCCCTGAGA AAGAGACTGA AATAATCAAG GATAAAATTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGAAGGA AGCAAAACCA GCTCCTGTAA AGAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAACCAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCT CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323020.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467345.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCAGA AATTATTAAG
    GAGAAATCAG CACCACCAAA GAAAGTTGAG GTTCCAAAAC AAAAGGCTCC TGAAAAGAAA
    GCACCTGAAA TAACCAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGCTGTA
    AAAGAGAAAG CTAAACCAGA GAAGAAAGAG GCTGAAGCCG TAAAGGAGAA AACGAAACCA
    GCCCCTGAGA AAGAGACTGA AATAATCAAG GATAAAATTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGAAGGA AGCAAAACCA GCTCCTGTAA AGAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAACCAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCT CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149682
    Clone ID Related Accession (Same CDS sequence) XM_021467346.1
    Accession Version XM_021467346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323021.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467346.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149683
    Clone ID Related Accession (Same CDS sequence) XM_021467347.1
    Accession Version XM_021467347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323022.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467347.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149684
    Clone ID Related Accession (Same CDS sequence) XM_021467348.1
    Accession Version XM_021467348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323023.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467348.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149685
    Clone ID Related Accession (Same CDS sequence) XM_021467349.1
    Accession Version XM_021467349.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323024.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467349.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149686
    Clone ID Related Accession (Same CDS sequence) XM_021467350.1
    Accession Version XM_021467350.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323025.1
    CDS338..8368
    Translation

    Target ORF information:

    RefSeq Version XM_021467350.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149687
    Clone ID Related Accession (Same CDS sequence) XM_021467351.1
    Accession Version XM_021467351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8013bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGAAATGAGG
    GAGGAGAAGG CTGAAGGTGT GGAGGAAGAG ATCGTGTTAC AACAGATAAA CATAACAGTA
    GAAAAGGTCA AGAAAGAAGA AACTGTCAAA AAGGAGGAAA AGGCTAAAAG AAAAGAAAAG
    GCTCATAAAC CAAAGGATGC AAAGATTGCC AAAGACAAAC CTAAAGCAGT CCCAGTAAAG
    CAAGAGGATG AAGTAGCCAA GAAAGAAACC AAACCAGCAC CATCTAAGAA AGAGGCTAAA
    ATTGTGAAGG AGAAACCCAA AGCACATCCT GCAAAGAAAG AAAAAGCTAA AGATATTAAG
    GAAAAGGCAA AATCTGGTCA AAAAGAAAAA GTTGCTGAGG CTGTCAAGGA AAAATCGAAG
    CCAGTAAAGA AAGAAGCTGA AAAAATTGAT GCCAAAGTCA AACCAGCAGC AGAAAAGAAA
    GTGGATGAAG CCCCCAAAGA GAAACCTAAA GTGAAGAAAG AGGCTGAAGT AATCAAAAAG
    ATTAAACTAG AGCCTGAGAG GAAAGAAGCT AAAACTCCCA AGGCCAAAAT TGAACCAGCT
    GGTTTGAAGA AAGATGCTGA AGCTGCTAAG GAGAAAGCAA AACCTGCTCC AGTCAAGAAA
    GGGCCTGAGG TTTCCAGAGA AAAAGCCAAA CCAGAGCCCG CAAAAAAAGA GCTTGAAGCG
    GTCAAAGAGA AAGCAAAACC AGCACCAGCT AAAAAAGATG AAGTCAAAGA GAAAGAAAAA
    CCAGCTCCTG TAAAGAAAGC TGAAGAAATT AAAAAGGCAA AAGCAGCTCC TTCAAGGAAA
    GAAGCTGTAA AGGAAAAACC CAAACCTGTT TCCGAAAAGA AAGAACCTGC TGTTGCAGTT
    GAAAAAGCCA AGCCAAAATC TACAAAGAAA GAAGCTGAAG TCGAGGAAAA AGTGAAACCT
    GCTCCTGAGA AGAAAGAAGC TGAAGTCATC ATGGTGAAAA CTAAACCAGT TCTTAAAAAG
    AAAGAGGCTG AAGTAATCAA AGAGGTTAAA CCAGCCCTTG AGAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA AAGTTAAACC AGCTCCTGTA AAGAAAGAGC CTGAAGTTGT TAAAGAAAAG
    GAACCTGAAC CAAAACCCAA AAGAGAGCCA GAAATTATTA AGGAGAAATC AGCACCACCA
    AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT CCTGAAAAGA AAGCACCTGA AATAACCAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGCTG TAAAAGAGAA AGCTAAACCA
    GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG AAAACGAAAC CAGCCCCTGA GAAAGAGACT
    GAAATAATCA AGGATAAAAT TAAAGCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG
    GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA ACTGAAGTCA TCAAAGAAAA AGAAAAACCA
    GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA AAGGAGAAAA CGAAACCAAC CTCTGAGAAG
    AAAGAGGCTG AAATAATTAA GGAGAAAGTT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGGAGG AAGCAAAACC AGCTCCTGTA AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAAACAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    CCTGAGAAGA AAGAGGCTGA AGTCATCAAG GAGATAGTTA AACAAGCTCC TGTGAAGAAA
    GAGGCTGAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AAGTTAAAGC AGCACCTCTG AAGAAAGAAG TCAAGGAGAA AGCAAAACCA
    GCTCCTGTAA AGAAAGAAGC TGAAGTCATC AAACAAAAAG CCAAACCAGC TCCTGAGAAG
    AAAGAGGCCA AAGTCATCAA GGAGAAAGTT AAACCGGCTC CTGTAAAGAT AGAGGCTAAA
    CCTGTAAAGG AGATAACTAA ACCAGCCCCT GAGAAGAAAG AGGCTGAAAT AATCAAGGAG
    AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA GTTGAAGTCA AGGAGGAAGC AAAACCAGCT
    CCTGAAAAGA AAGAGGCTGA AGTCATCAAG GAGAAAGTTA AACCTGCTCC TGTGAGGAAA
    GAAGCTGAAG AAATAAAGGA AAAAGCTGAA GCAGTTTATG AGAAAAAAGA GGCTGAAGTA
    GCCAAGGAGA TAGTTAAACC AACTCCTTTA AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG
    GCTCCTGAAT CAAAACCCCA AAAAGAGGCT GAAATTATCA AAGAGAAAGT TAAACCTGCA
    CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA GTACCAAAAC CCAAAAAAGA GGCTGAACTC
    GCCAAAGAGA AAGTTAAACC TGCACCTGTA AAGAAAGAAG AAAAACCTAA GGCAGCACCA
    AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA CGTACACCAG CTCCTGAAAA GAAAGTGGTA
    GAAGTCATCA AGGAGAAAGT CAAACCAGCC CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA
    GTTAGACCTG CTCCTGAAAA GAAAGAGCCT GAAGTTGCTA AAGAAAAGGC TCCTGAACCA
    AAACCCCAAA AAGAGGTTGA AGTCATCAAA GAGAAAGCTA AACCAGCCCT TAAGAAGAAA
    GAGCCTAAAG TCAAGGAAAA GGTTCAACCA GCTCCTATAA AGAAAGAGCC AGAGCTTCCC
    AAAGAAAAGG TGCCTGAACC AACACCAGAA CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG
    GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT GAGATTACTA AAGAAAAACC AAAGCCAGCA
    CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA GCTACACCTG CTCCTGTGCA CAAAGAGGTT
    GAAGTGTTCA AGGAGAAAGT TAAACAAGCT CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA
    GAAAAGGCTC CTGAACCAAA ACCTAAAAAG ACTGAAGTCA TCAAAGCGAA AGTAAAACCA
    GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG GAGAAAATTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATAAAGGA GAGAATTAAA CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCC AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA
    GTGAAACCAG CGCCAGAGAA GAAAGCACCC GAAATAACCA AAGAAAAACC AAAGCCAGCA
    GCAAAGAAAG TAGCTGAAGT CATCAAAGAC AGAACTAAAC CAGTCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA GTTCCCGAGA AGAAAGAGGC TAAAGTCATT
    GAAAAGAAAG TTATACCAGC TCCCGTAAAG AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT
    CCTGAACTGA CAACAAAAAA AGCACCTGAG ATAACCAAAG AAAAACCGAA GCCAGTACCA
    AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA GCTAAACTAG CTCCTGAAAA GAAAGAGGTT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCGTTAAAGA AAGAGCCTGA GGTTCATAAG
    GAAAAAGCAC CTGAACCAAA ATCAGAACCT ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG
    GCAAAACCAG TTGCTAAAGA AAAGGAACCT GAAATAACCA AAGAAAAGCC AAAACCAGCA
    CCAAAGAAAG AAGCTGTAGT CATCAAAGAA AAAGCTGTAC CAGCCATCCT GAAGGAAGTG
    GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA
    GTTAAACCAG CTTCTATAAT GAAAGAGCCT GAAGTTGTGA AACAAAAGAC TCCTGAACCG
    AAACCCAAAA AAGAGCCTGA AGTTGTGAAA GAAAAGGCTC CTGAACCTAA ACCCAAAAAA
    GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA CCAGCACCTC AGAAGAAAGA AGTCATCAAA
    GAAAAAGTCA AACTTGCTCC TGTAAAGAAA GTGCCCGAAG TTGTCGAAGA AAAGGCACTT
    AAACCAGAAC CCAAAAAAGA TGAAGTTGGT CTTATTGGTC TGGACCTGGA GCTCCTAAAC
    TCCATCAACC AAAAACTGTC TCTCCTGGAC TTGCTAAGGA AGGACATTGG AGAAATGAAA
    AGCGATCTGG AGTCCACTCA GAATCAAATC ACACTTTTAC GGATGGACAA CAGAACGATC
    AAAGAGCCAG AGACTATTGG TGTGAAAGTA AAACCAGCAG TCAAGGAAAA AGCAAAAAGA
    GACCACGCTC TGATCAATGC TACTAGGACT GCACATATTA AAACAGAGCG TGAATCTCTA
    AAAAACATCA CGAAACCAGT GCCTGCAAAG AAAGCCAAAG AGGCTCCAAA GGTTAAGATT
    GCGCTGGAGC CTAAAAAGAA AGGAATCATC CCTGAGAAGG TCAAAGAAGT AAAGGAAACT
    TTACAGAAAG AACCTGAGTT GGTCAAAGAT AAAGTAGTTG AGCCAATAGA GAAGGAAATT
    GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG CCTGAAATCA CCAAAGATAT TCCAGAGGTG
    GAGGAAGAGG ACATTCCCTA CTTCCAGTGT TTCTTTGTGG ATGAGGATGA CACTCAGTAT
    CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323026.1
    CDS338..8350
    Translation

    Target ORF information:

    RefSeq Version XM_021467351.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGAAATGAGG
    GAGGAGAAGG CTGAAGGTGT GGAGGAAGAG ATCGTGTTAC AACAGATAAA CATAACAGTA
    GAAAAGGTCA AGAAAGAAGA AACTGTCAAA AAGGAGGAAA AGGCTAAAAG AAAAGAAAAG
    GCTCATAAAC CAAAGGATGC AAAGATTGCC AAAGACAAAC CTAAAGCAGT CCCAGTAAAG
    CAAGAGGATG AAGTAGCCAA GAAAGAAACC AAACCAGCAC CATCTAAGAA AGAGGCTAAA
    ATTGTGAAGG AGAAACCCAA AGCACATCCT GCAAAGAAAG AAAAAGCTAA AGATATTAAG
    GAAAAGGCAA AATCTGGTCA AAAAGAAAAA GTTGCTGAGG CTGTCAAGGA AAAATCGAAG
    CCAGTAAAGA AAGAAGCTGA AAAAATTGAT GCCAAAGTCA AACCAGCAGC AGAAAAGAAA
    GTGGATGAAG CCCCCAAAGA GAAACCTAAA GTGAAGAAAG AGGCTGAAGT AATCAAAAAG
    ATTAAACTAG AGCCTGAGAG GAAAGAAGCT AAAACTCCCA AGGCCAAAAT TGAACCAGCT
    GGTTTGAAGA AAGATGCTGA AGCTGCTAAG GAGAAAGCAA AACCTGCTCC AGTCAAGAAA
    GGGCCTGAGG TTTCCAGAGA AAAAGCCAAA CCAGAGCCCG CAAAAAAAGA GCTTGAAGCG
    GTCAAAGAGA AAGCAAAACC AGCACCAGCT AAAAAAGATG AAGTCAAAGA GAAAGAAAAA
    CCAGCTCCTG TAAAGAAAGC TGAAGAAATT AAAAAGGCAA AAGCAGCTCC TTCAAGGAAA
    GAAGCTGTAA AGGAAAAACC CAAACCTGTT TCCGAAAAGA AAGAACCTGC TGTTGCAGTT
    GAAAAAGCCA AGCCAAAATC TACAAAGAAA GAAGCTGAAG TCGAGGAAAA AGTGAAACCT
    GCTCCTGAGA AGAAAGAAGC TGAAGTCATC ATGGTGAAAA CTAAACCAGT TCTTAAAAAG
    AAAGAGGCTG AAGTAATCAA AGAGGTTAAA CCAGCCCTTG AGAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA AAGTTAAACC AGCTCCTGTA AAGAAAGAGC CTGAAGTTGT TAAAGAAAAG
    GAACCTGAAC CAAAACCCAA AAGAGAGCCA GAAATTATTA AGGAGAAATC AGCACCACCA
    AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT CCTGAAAAGA AAGCACCTGA AATAACCAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGCTG TAAAAGAGAA AGCTAAACCA
    GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG AAAACGAAAC CAGCCCCTGA GAAAGAGACT
    GAAATAATCA AGGATAAAAT TAAAGCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG
    GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA ACTGAAGTCA TCAAAGAAAA AGAAAAACCA
    GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA AAGGAGAAAA CGAAACCAAC CTCTGAGAAG
    AAAGAGGCTG AAATAATTAA GGAGAAAGTT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGGAGG AAGCAAAACC AGCTCCTGTA AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAAACAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    CCTGAGAAGA AAGAGGCTGA AGTCATCAAG GAGATAGTTA AACAAGCTCC TGTGAAGAAA
    GAGGCTGAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AAGTTAAAGC AGCACCTCTG AAGAAAGAAG TCAAGGAGAA AGCAAAACCA
    GCTCCTGTAA AGAAAGAAGC TGAAGTCATC AAACAAAAAG CCAAACCAGC TCCTGAGAAG
    AAAGAGGCCA AAGTCATCAA GGAGAAAGTT AAACCGGCTC CTGTAAAGAT AGAGGCTAAA
    CCTGTAAAGG AGATAACTAA ACCAGCCCCT GAGAAGAAAG AGGCTGAAAT AATCAAGGAG
    AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA GTTGAAGTCA AGGAGGAAGC AAAACCAGCT
    CCTGAAAAGA AAGAGGCTGA AGTCATCAAG GAGAAAGTTA AACCTGCTCC TGTGAGGAAA
    GAAGCTGAAG AAATAAAGGA AAAAGCTGAA GCAGTTTATG AGAAAAAAGA GGCTGAAGTA
    GCCAAGGAGA TAGTTAAACC AACTCCTTTA AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG
    GCTCCTGAAT CAAAACCCCA AAAAGAGGCT GAAATTATCA AAGAGAAAGT TAAACCTGCA
    CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA GTACCAAAAC CCAAAAAAGA GGCTGAACTC
    GCCAAAGAGA AAGTTAAACC TGCACCTGTA AAGAAAGAAG AAAAACCTAA GGCAGCACCA
    AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA CGTACACCAG CTCCTGAAAA GAAAGTGGTA
    GAAGTCATCA AGGAGAAAGT CAAACCAGCC CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA
    GTTAGACCTG CTCCTGAAAA GAAAGAGCCT GAAGTTGCTA AAGAAAAGGC TCCTGAACCA
    AAACCCCAAA AAGAGGTTGA AGTCATCAAA GAGAAAGCTA AACCAGCCCT TAAGAAGAAA
    GAGCCTAAAG TCAAGGAAAA GGTTCAACCA GCTCCTATAA AGAAAGAGCC AGAGCTTCCC
    AAAGAAAAGG TGCCTGAACC AACACCAGAA CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG
    GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT GAGATTACTA AAGAAAAACC AAAGCCAGCA
    CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA GCTACACCTG CTCCTGTGCA CAAAGAGGTT
    GAAGTGTTCA AGGAGAAAGT TAAACAAGCT CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA
    GAAAAGGCTC CTGAACCAAA ACCTAAAAAG ACTGAAGTCA TCAAAGCGAA AGTAAAACCA
    GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG GAGAAAATTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATAAAGGA GAGAATTAAA CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCC AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA
    GTGAAACCAG CGCCAGAGAA GAAAGCACCC GAAATAACCA AAGAAAAACC AAAGCCAGCA
    GCAAAGAAAG TAGCTGAAGT CATCAAAGAC AGAACTAAAC CAGTCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA GTTCCCGAGA AGAAAGAGGC TAAAGTCATT
    GAAAAGAAAG TTATACCAGC TCCCGTAAAG AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT
    CCTGAACTGA CAACAAAAAA AGCACCTGAG ATAACCAAAG AAAAACCGAA GCCAGTACCA
    AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA GCTAAACTAG CTCCTGAAAA GAAAGAGGTT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCGTTAAAGA AAGAGCCTGA GGTTCATAAG
    GAAAAAGCAC CTGAACCAAA ATCAGAACCT ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG
    GCAAAACCAG TTGCTAAAGA AAAGGAACCT GAAATAACCA AAGAAAAGCC AAAACCAGCA
    CCAAAGAAAG AAGCTGTAGT CATCAAAGAA AAAGCTGTAC CAGCCATCCT GAAGGAAGTG
    GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA
    GTTAAACCAG CTTCTATAAT GAAAGAGCCT GAAGTTGTGA AACAAAAGAC TCCTGAACCG
    AAACCCAAAA AAGAGCCTGA AGTTGTGAAA GAAAAGGCTC CTGAACCTAA ACCCAAAAAA
    GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA CCAGCACCTC AGAAGAAAGA AGTCATCAAA
    GAAAAAGTCA AACTTGCTCC TGTAAAGAAA GTGCCCGAAG TTGTCGAAGA AAAGGCACTT
    AAACCAGAAC CCAAAAAAGA TGAAGTTGGT CTTATTGGTC TGGACCTGGA GCTCCTAAAC
    TCCATCAACC AAAAACTGTC TCTCCTGGAC TTGCTAAGGA AGGACATTGG AGAAATGAAA
    AGCGATCTGG AGTCCACTCA GAATCAAATC ACACTTTTAC GGATGGACAA CAGAACGATC
    AAAGAGCCAG AGACTATTGG TGTGAAAGTA AAACCAGCAG TCAAGGAAAA AGCAAAAAGA
    GACCACGCTC TGATCAATGC TACTAGGACT GCACATATTA AAACAGAGCG TGAATCTCTA
    AAAAACATCA CGAAACCAGT GCCTGCAAAG AAAGCCAAAG AGGCTCCAAA GGTTAAGATT
    GCGCTGGAGC CTAAAAAGAA AGGAATCATC CCTGAGAAGG TCAAAGAAGT AAAGGAAACT
    TTACAGAAAG AACCTGAGTT GGTCAAAGAT AAAGTAGTTG AGCCAATAGA GAAGGAAATT
    GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG CCTGAAATCA CCAAAGATAT TCCAGAGGTG
    GAGGAAGAGG ACATTCCCTA CTTCCAGTGT TTCTTTGTGG ATGAGGATGA CACTCAGTAT
    CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149688
    Clone ID Related Accession (Same CDS sequence) XM_021467352.1
    Accession Version XM_021467352.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7998bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGAGGCTGA AGCTGTAAAA GAGAAAGCTA AACCAGAGAA GAAAGAGGCT
    GAAGCCGTAA AGGAGAAAAC GAAACCAGCC CCTGAGAAAG AGACTGAAAT AATCAAGGAT
    AAAATTAAAG CAGCTCCTGT GAAGAAAGAA GTTGAAGTCA AGAAGGAAGC AAAACCAGCT
    CCTGTAAAGA AAGAAACTGA AGTCATCAAA GAAAAAGAAA AACCAGCTCC TGAGAAGAAA
    GAGGCTGAAG CTGTAAAGGA GAAAACGAAA CCAACCTCTG AGAAGAAAGA GGCTGAAATA
    ATTAAGGAGA AAGTTAAAGC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGTAAAAAA AGAAACTGAA GTCATCAAAG AAAAAGAAAA AACAGCTCCT
    GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG AAAACGAAAC CAACCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAT AGTTAAACAA GCTCCTGTGA AGAAAGAGGC TGAACCTGTA
    AAGGAGATAA CTAAACCAGC CCCTGAGAAG AAAGAGGCTG AAATAATCAA GGAGAAAGTT
    AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG GAGAAAGCAA AACCAGCTCC TGTAAAGAAA
    GAAGCTGAAG TCATCAAACA AAAAGCCAAA CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC
    ATCAAGGAGA AAGTTAAACC GGCTCCTGTA AAGATAGAGG CTAAACCTGT AAAGGAGATA
    ACTAAACCAG CCCCTGAGAA GAAAGAGGCT GAAATAATCA AGGAGAAGGT CAAATCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCT GCTCCTGTGA GGAAAGAAGC TGAAGAAATA
    AAGGAAAAAG CTGAAGCAGT TTATGAGAAA AAAGAGGCTG AAGTAGCCAA GGAGATAGTT
    AAACCAACTC CTTTAAAGAA GGAGCCCGAA GTTGTGAAGG AAAAGGCTCC TGAATCAAAA
    CCCCAAAAAG AGGCTGAAAT TATCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAG
    CCTGAAGTTG CAAAAGTACC AAAACCCAAA AAAGAGGCTG AACTCGCCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAAGAAAAA CCTAAGGCAG CACCAAAGAA AGAGGCTGAA
    GCTATTAAGG AGAAACGTAC ACCAGCTCCT GAAAAGAAAG TGGTAGAAGT CATCAAGGAG
    AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT
    GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA AAGGCTCCTG AACCAAAACC CCAAAAAGAG
    GTTGAAGTCA TCAAAGAGAA AGCTAAACCA GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG
    GAAAAGGTTC AACCAGCTCC TATAAAGAAA GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT
    GAACCAACAC CAGAACCTGA GAGAGTTGAA GCTCCAAAAG AAAAGGCAAA AGTAATCCCT
    AGGGAAAAAG TAGCTGAGAT TACTAAAGAA AAACCAAAGC CAGCACCAAA GAAAGAGGCT
    GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT GTGCACAAAG AGGTTGAAGT GTTCAAGGAG
    AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA
    CCAAAACCTA AAAAGACTGA AGTCATCAAA GCGAAAGTAA AACCAGCCCC TGAGAAAAAA
    GAGGCTGAAG TGAAGGAGAA AATTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATA
    AAGGAGAGAA TTAAACCTGC TCCTGTAAAG AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT
    CCTGAACCAA AACCCAAAAA AGAACCTGAA GTTATCAAAG AGAAAGTGAA ACCAGCGCCA
    GAGAAGAAAG CACCCGAAAT AACCAAAGAA AAACCAAAGC CAGCAGCAAA GAAAGTAGCT
    GAAGTCATCA AAGACAGAAC TAAACCAGTC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAACCAA AAGCAGTTCC CGAGAAGAAA GAGGCTAAAG TCATTGAAAA GAAAGTTATA
    CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT GTTAAGGAAA AGGCTCCTGA ACTGACAACA
    AAAAAAGCAC CTGAGATAAC CAAAGAAAAA CCGAAGCCAG TACCAAAGAA AGAGGCTGAA
    GTCATTAAAG AGAAAGCTAA ACTAGCTCCT GAAAAGAAAG AGGTTGAAGT CATCAAGGAG
    AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG CCTGAGGTTC ATAAGGAAAA AGCACCTGAA
    CCAAAATCAG AACCTACAAA AAAAGTTGAG GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT
    AAAGAAAAGG AACCTGAAAT AACCAAAGAA AAGCCAAAAC CAGCACCAAA GAAAGAAGCT
    GTAGTCATCA AAGAAAAAGC TGTACCAGCC ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA
    GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT GAAATTAAGG AGAAAGTTAA ACCAGCTTCT
    ATAATGAAAG AGCCTGAAGT TGTGAAACAA AAGACTCCTG AACCGAAACC CAAAAAAGAG
    CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA CCTAAACCCA AAAAAGAGCC TGAAGTTGCT
    AAAGAGAAAG CCAAACCAGC ACCTCAGAAG AAAGAAGTCA TCAAAGAAAA AGTCAAACTT
    GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC GAAGAAAAGG CACTTAAACC AGAACCCAAA
    AAAGATGAAG TTGGTCTTAT TGGTCTGGAC CTGGAGCTCC TAAACTCCAT CAACCAAAAA
    CTGTCTCTCC TGGACTTGCT AAGGAAGGAC ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC
    ACTCAGAATC AAATCACACT TTTACGGATG GACAACAGAA CGATCAAAGA GCCAGAGACT
    ATTGGTGTGA AAGTAAAACC AGCAGTCAAG GAAAAAGCAA AAAGAGACCA CGCTCTGATC
    AATGCTACTA GGACTGCACA TATTAAAACA GAGCGTGAAT CTCTAAAAAA CATCACGAAA
    CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT CCAAAGGTTA AGATTGCGCT GGAGCCTAAA
    AAGAAAGGAA TCATCCCTGA GAAGGTCAAA GAAGTAAAGG AAACTTTACA GAAAGAACCT
    GAGTTGGTCA AAGATAAAGT AGTTGAGCCA ATAGAGAAGG AAATTGTTGT TGAGAAGGAA
    GTCAAAGGAG AAGAGCCTGA AATCACCAAA GATATTCCAG AGGTGGAGGA AGAGGACATT
    CCCTACTTCC AGTGTTTCTT TGTGGATGAG GATGACACTC AGTATCCGTT CTTCCCTTTC
    TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323027.1
    CDS338..8335
    Translation

    Target ORF information:

    RefSeq Version XM_021467352.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGAGGCTGA AGCTGTAAAA GAGAAAGCTA AACCAGAGAA GAAAGAGGCT
    GAAGCCGTAA AGGAGAAAAC GAAACCAGCC CCTGAGAAAG AGACTGAAAT AATCAAGGAT
    AAAATTAAAG CAGCTCCTGT GAAGAAAGAA GTTGAAGTCA AGAAGGAAGC AAAACCAGCT
    CCTGTAAAGA AAGAAACTGA AGTCATCAAA GAAAAAGAAA AACCAGCTCC TGAGAAGAAA
    GAGGCTGAAG CTGTAAAGGA GAAAACGAAA CCAACCTCTG AGAAGAAAGA GGCTGAAATA
    ATTAAGGAGA AAGTTAAAGC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGTAAAAAA AGAAACTGAA GTCATCAAAG AAAAAGAAAA AACAGCTCCT
    GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG AAAACGAAAC CAACCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAT AGTTAAACAA GCTCCTGTGA AGAAAGAGGC TGAACCTGTA
    AAGGAGATAA CTAAACCAGC CCCTGAGAAG AAAGAGGCTG AAATAATCAA GGAGAAAGTT
    AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG GAGAAAGCAA AACCAGCTCC TGTAAAGAAA
    GAAGCTGAAG TCATCAAACA AAAAGCCAAA CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC
    ATCAAGGAGA AAGTTAAACC GGCTCCTGTA AAGATAGAGG CTAAACCTGT AAAGGAGATA
    ACTAAACCAG CCCCTGAGAA GAAAGAGGCT GAAATAATCA AGGAGAAGGT CAAATCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCT GCTCCTGTGA GGAAAGAAGC TGAAGAAATA
    AAGGAAAAAG CTGAAGCAGT TTATGAGAAA AAAGAGGCTG AAGTAGCCAA GGAGATAGTT
    AAACCAACTC CTTTAAAGAA GGAGCCCGAA GTTGTGAAGG AAAAGGCTCC TGAATCAAAA
    CCCCAAAAAG AGGCTGAAAT TATCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAG
    CCTGAAGTTG CAAAAGTACC AAAACCCAAA AAAGAGGCTG AACTCGCCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAAGAAAAA CCTAAGGCAG CACCAAAGAA AGAGGCTGAA
    GCTATTAAGG AGAAACGTAC ACCAGCTCCT GAAAAGAAAG TGGTAGAAGT CATCAAGGAG
    AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT
    GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA AAGGCTCCTG AACCAAAACC CCAAAAAGAG
    GTTGAAGTCA TCAAAGAGAA AGCTAAACCA GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG
    GAAAAGGTTC AACCAGCTCC TATAAAGAAA GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT
    GAACCAACAC CAGAACCTGA GAGAGTTGAA GCTCCAAAAG AAAAGGCAAA AGTAATCCCT
    AGGGAAAAAG TAGCTGAGAT TACTAAAGAA AAACCAAAGC CAGCACCAAA GAAAGAGGCT
    GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT GTGCACAAAG AGGTTGAAGT GTTCAAGGAG
    AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA
    CCAAAACCTA AAAAGACTGA AGTCATCAAA GCGAAAGTAA AACCAGCCCC TGAGAAAAAA
    GAGGCTGAAG TGAAGGAGAA AATTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATA
    AAGGAGAGAA TTAAACCTGC TCCTGTAAAG AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT
    CCTGAACCAA AACCCAAAAA AGAACCTGAA GTTATCAAAG AGAAAGTGAA ACCAGCGCCA
    GAGAAGAAAG CACCCGAAAT AACCAAAGAA AAACCAAAGC CAGCAGCAAA GAAAGTAGCT
    GAAGTCATCA AAGACAGAAC TAAACCAGTC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAACCAA AAGCAGTTCC CGAGAAGAAA GAGGCTAAAG TCATTGAAAA GAAAGTTATA
    CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT GTTAAGGAAA AGGCTCCTGA ACTGACAACA
    AAAAAAGCAC CTGAGATAAC CAAAGAAAAA CCGAAGCCAG TACCAAAGAA AGAGGCTGAA
    GTCATTAAAG AGAAAGCTAA ACTAGCTCCT GAAAAGAAAG AGGTTGAAGT CATCAAGGAG
    AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG CCTGAGGTTC ATAAGGAAAA AGCACCTGAA
    CCAAAATCAG AACCTACAAA AAAAGTTGAG GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT
    AAAGAAAAGG AACCTGAAAT AACCAAAGAA AAGCCAAAAC CAGCACCAAA GAAAGAAGCT
    GTAGTCATCA AAGAAAAAGC TGTACCAGCC ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA
    GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT GAAATTAAGG AGAAAGTTAA ACCAGCTTCT
    ATAATGAAAG AGCCTGAAGT TGTGAAACAA AAGACTCCTG AACCGAAACC CAAAAAAGAG
    CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA CCTAAACCCA AAAAAGAGCC TGAAGTTGCT
    AAAGAGAAAG CCAAACCAGC ACCTCAGAAG AAAGAAGTCA TCAAAGAAAA AGTCAAACTT
    GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC GAAGAAAAGG CACTTAAACC AGAACCCAAA
    AAAGATGAAG TTGGTCTTAT TGGTCTGGAC CTGGAGCTCC TAAACTCCAT CAACCAAAAA
    CTGTCTCTCC TGGACTTGCT AAGGAAGGAC ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC
    ACTCAGAATC AAATCACACT TTTACGGATG GACAACAGAA CGATCAAAGA GCCAGAGACT
    ATTGGTGTGA AAGTAAAACC AGCAGTCAAG GAAAAAGCAA AAAGAGACCA CGCTCTGATC
    AATGCTACTA GGACTGCACA TATTAAAACA GAGCGTGAAT CTCTAAAAAA CATCACGAAA
    CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT CCAAAGGTTA AGATTGCGCT GGAGCCTAAA
    AAGAAAGGAA TCATCCCTGA GAAGGTCAAA GAAGTAAAGG AAACTTTACA GAAAGAACCT
    GAGTTGGTCA AAGATAAAGT AGTTGAGCCA ATAGAGAAGG AAATTGTTGT TGAGAAGGAA
    GTCAAAGGAG AAGAGCCTGA AATCACCAAA GATATTCCAG AGGTGGAGGA AGAGGACATT
    CCCTACTTCC AGTGTTTCTT TGTGGATGAG GATGACACTC AGTATCCGTT CTTCCCTTTC
    TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149689
    Clone ID Related Accession (Same CDS sequence) XM_021467353.1
    Accession Version XM_021467353.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA AGAAAAACCT AAGGCAGCAC CAAAGAAAGA GGCTGAAGCT
    ATTAAGGAGA AACGTACACC AGCTCCTGAA AAGAAAGTGG TAGAAGTCAT CAAGGAGAAA
    GTCAAACCAG CCCCTGAGAA AAAAGCGGTT CTCAAGGAGA AAGTTAGACC TGCTCCTGAA
    AAGAAAGAGC CTGAAGTTGC TAAAGAAAAG GCTCCTGAAC CAAAACCCCA AAAAGAGGTT
    GAAGTCATCA AAGAGAAAGC TAAACCAGCC CTTAAGAAGA AAGAGCCTAA AGTCAAGGAA
    AAGGTTCAAC CAGCTCCTAT AAAGAAAGAG CCAGAGCTTC CCAAAGAAAA GGTGCCTGAA
    CCAACACCAG AACCTGAGAG AGTTGAAGCT CCAAAAGAAA AGGCAAAAGT AATCCCTAGG
    GAAAAAGTAG CTGAGATTAC TAAAGAAAAA CCAAAGCCAG CACCAAAGAA AGAGGCTGAA
    GTAAAGGAGA AAGCTACACC TGCTCCTGTG CACAAAGAGG TTGAAGTGTT CAAGGAGAAA
    GTTAAACAAG CTCCTGTAAA GAAAGAGCCA GAAGTTCCTA AAGAAAAGGC TCCTGAACCA
    AAACCTAAAA AGACTGAAGT CATCAAAGCG AAAGTAAAAC CAGCCCCTGA GAAAAAAGAG
    GCTGAAGTGA AGGAGAAAAT TAAACCAGTC CCTGAGAAGA AAGAGGCTGA AGTCATAAAG
    GAGAGAATTA AACCTGCTCC TGTAAAGAAA GAGCCTGAAG TTCCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCAAAAAAGA ACCTGAAGTT ATCAAAGAGA AAGTGAAACC AGCGCCAGAG
    AAGAAAGCAC CCGAAATAAC CAAAGAAAAA CCAAAGCCAG CAGCAAAGAA AGTAGCTGAA
    GTCATCAAAG ACAGAACTAA ACCAGTCCCT GAGAAGAAAG AGGCTGAAGT CATCAAGGAG
    AAACCAAAAG CAGTTCCCGA GAAGAAAGAG GCTAAAGTCA TTGAAAAGAA AGTTATACCA
    GCTCCCGTAA AGAAAGAGCC TGAAGTTGTT AAGGAAAAGG CTCCTGAACT GACAACAAAA
    AAAGCACCTG AGATAACCAA AGAAAAACCG AAGCCAGTAC CAAAGAAAGA GGCTGAAGTC
    ATTAAAGAGA AAGCTAAACT AGCTCCTGAA AAGAAAGAGG TTGAAGTCAT CAAGGAGAAA
    GTTAAACCAG CTCCGTTAAA GAAAGAGCCT GAGGTTCATA AGGAAAAAGC ACCTGAACCA
    AAATCAGAAC CTACAAAAAA AGTTGAGGTT CCAAAGGAAA AGGCAAAACC AGTTGCTAAA
    GAAAAGGAAC CTGAAATAAC CAAAGAAAAG CCAAAACCAG CACCAAAGAA AGAAGCTGTA
    GTCATCAAAG AAAAAGCTGT ACCAGCCATC CTGAAGGAAG TGGCTGAAGT AAAGAAAGTT
    AAAGCAGCTC CTGAGAAGAA AGTGGCTGAA ATTAAGGAGA AAGTTAAACC AGCTTCTATA
    ATGAAAGAGC CTGAAGTTGT GAAACAAAAG ACTCCTGAAC CGAAACCCAA AAAAGAGCCT
    GAAGTTGTGA AAGAAAAGGC TCCTGAACCT AAACCCAAAA AAGAGCCTGA AGTTGCTAAA
    GAGAAAGCCA AACCAGCACC TCAGAAGAAA GAAGTCATCA AAGAAAAAGT CAAACTTGCT
    CCTGTAAAGA AAGTGCCCGA AGTTGTCGAA GAAAAGGCAC TTAAACCAGA ACCCAAAAAA
    GATGAAGTTG GTCTTATTGG TCTGGACCTG GAGCTCCTAA ACTCCATCAA CCAAAAACTG
    TCTCTCCTGG ACTTGCTAAG GAAGGACATT GGAGAAATGA AAAGCGATCT GGAGTCCACT
    CAGAATCAAA TCACACTTTT ACGGATGGAC AACAGAACGA TCAAAGAGCC AGAGACTATT
    GGTGTGAAAG TAAAACCAGC AGTCAAGGAA AAAGCAAAAA GAGACCACGC TCTGATCAAT
    GCTACTAGGA CTGCACATAT TAAAACAGAG CGTGAATCTC TAAAAAACAT CACGAAACCA
    GTGCCTGCAA AGAAAGCCAA AGAGGCTCCA AAGGTTAAGA TTGCGCTGGA GCCTAAAAAG
    AAAGGAATCA TCCCTGAGAA GGTCAAAGAA GTAAAGGAAA CTTTACAGAA AGAACCTGAG
    TTGGTCAAAG ATAAAGTAGT TGAGCCAATA GAGAAGGAAA TTGTTGTTGA GAAGGAAGTC
    AAAGGAGAAG AGCCTGAAAT CACCAAAGAT ATTCCAGAGG TGGAGGAAGA GGACATTCCC
    TACTTCCAGT GTTTCTTTGT GGATGAGGAT GACACTCAGT ATCCGTTCTT CCCTTTCTCT
    CCACCGCTTT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323028.1
    CDS338..8332
    Translation

    Target ORF information:

    RefSeq Version XM_021467353.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA AGAAAAACCT AAGGCAGCAC CAAAGAAAGA GGCTGAAGCT
    ATTAAGGAGA AACGTACACC AGCTCCTGAA AAGAAAGTGG TAGAAGTCAT CAAGGAGAAA
    GTCAAACCAG CCCCTGAGAA AAAAGCGGTT CTCAAGGAGA AAGTTAGACC TGCTCCTGAA
    AAGAAAGAGC CTGAAGTTGC TAAAGAAAAG GCTCCTGAAC CAAAACCCCA AAAAGAGGTT
    GAAGTCATCA AAGAGAAAGC TAAACCAGCC CTTAAGAAGA AAGAGCCTAA AGTCAAGGAA
    AAGGTTCAAC CAGCTCCTAT AAAGAAAGAG CCAGAGCTTC CCAAAGAAAA GGTGCCTGAA
    CCAACACCAG AACCTGAGAG AGTTGAAGCT CCAAAAGAAA AGGCAAAAGT AATCCCTAGG
    GAAAAAGTAG CTGAGATTAC TAAAGAAAAA CCAAAGCCAG CACCAAAGAA AGAGGCTGAA
    GTAAAGGAGA AAGCTACACC TGCTCCTGTG CACAAAGAGG TTGAAGTGTT CAAGGAGAAA
    GTTAAACAAG CTCCTGTAAA GAAAGAGCCA GAAGTTCCTA AAGAAAAGGC TCCTGAACCA
    AAACCTAAAA AGACTGAAGT CATCAAAGCG AAAGTAAAAC CAGCCCCTGA GAAAAAAGAG
    GCTGAAGTGA AGGAGAAAAT TAAACCAGTC CCTGAGAAGA AAGAGGCTGA AGTCATAAAG
    GAGAGAATTA AACCTGCTCC TGTAAAGAAA GAGCCTGAAG TTCCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCAAAAAAGA ACCTGAAGTT ATCAAAGAGA AAGTGAAACC AGCGCCAGAG
    AAGAAAGCAC CCGAAATAAC CAAAGAAAAA CCAAAGCCAG CAGCAAAGAA AGTAGCTGAA
    GTCATCAAAG ACAGAACTAA ACCAGTCCCT GAGAAGAAAG AGGCTGAAGT CATCAAGGAG
    AAACCAAAAG CAGTTCCCGA GAAGAAAGAG GCTAAAGTCA TTGAAAAGAA AGTTATACCA
    GCTCCCGTAA AGAAAGAGCC TGAAGTTGTT AAGGAAAAGG CTCCTGAACT GACAACAAAA
    AAAGCACCTG AGATAACCAA AGAAAAACCG AAGCCAGTAC CAAAGAAAGA GGCTGAAGTC
    ATTAAAGAGA AAGCTAAACT AGCTCCTGAA AAGAAAGAGG TTGAAGTCAT CAAGGAGAAA
    GTTAAACCAG CTCCGTTAAA GAAAGAGCCT GAGGTTCATA AGGAAAAAGC ACCTGAACCA
    AAATCAGAAC CTACAAAAAA AGTTGAGGTT CCAAAGGAAA AGGCAAAACC AGTTGCTAAA
    GAAAAGGAAC CTGAAATAAC CAAAGAAAAG CCAAAACCAG CACCAAAGAA AGAAGCTGTA
    GTCATCAAAG AAAAAGCTGT ACCAGCCATC CTGAAGGAAG TGGCTGAAGT AAAGAAAGTT
    AAAGCAGCTC CTGAGAAGAA AGTGGCTGAA ATTAAGGAGA AAGTTAAACC AGCTTCTATA
    ATGAAAGAGC CTGAAGTTGT GAAACAAAAG ACTCCTGAAC CGAAACCCAA AAAAGAGCCT
    GAAGTTGTGA AAGAAAAGGC TCCTGAACCT AAACCCAAAA AAGAGCCTGA AGTTGCTAAA
    GAGAAAGCCA AACCAGCACC TCAGAAGAAA GAAGTCATCA AAGAAAAAGT CAAACTTGCT
    CCTGTAAAGA AAGTGCCCGA AGTTGTCGAA GAAAAGGCAC TTAAACCAGA ACCCAAAAAA
    GATGAAGTTG GTCTTATTGG TCTGGACCTG GAGCTCCTAA ACTCCATCAA CCAAAAACTG
    TCTCTCCTGG ACTTGCTAAG GAAGGACATT GGAGAAATGA AAAGCGATCT GGAGTCCACT
    CAGAATCAAA TCACACTTTT ACGGATGGAC AACAGAACGA TCAAAGAGCC AGAGACTATT
    GGTGTGAAAG TAAAACCAGC AGTCAAGGAA AAAGCAAAAA GAGACCACGC TCTGATCAAT
    GCTACTAGGA CTGCACATAT TAAAACAGAG CGTGAATCTC TAAAAAACAT CACGAAACCA
    GTGCCTGCAA AGAAAGCCAA AGAGGCTCCA AAGGTTAAGA TTGCGCTGGA GCCTAAAAAG
    AAAGGAATCA TCCCTGAGAA GGTCAAAGAA GTAAAGGAAA CTTTACAGAA AGAACCTGAG
    TTGGTCAAAG ATAAAGTAGT TGAGCCAATA GAGAAGGAAA TTGTTGTTGA GAAGGAAGTC
    AAAGGAGAAG AGCCTGAAAT CACCAAAGAT ATTCCAGAGG TGGAGGAAGA GGACATTCCC
    TACTTCCAGT GTTTCTTTGT GGATGAGGAT GACACTCAGT ATCCGTTCTT CCCTTTCTCT
    CCACCGCTTT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149690
    Clone ID Related Accession (Same CDS sequence) XM_021467354.1
    Accession Version XM_021467354.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGTGGC TGAAATTAAG GAGAAAGTTA AACCAGCTTC TATAATGAAA
    GAGCCTGAAG TTGTGAAACA AAAGACTCCT GAACCGAAAC CCAAAAAAGA GCCTGAAGTT
    GTGAAAGAAA AGGCTCCTGA ACCTAAACCC AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA
    GCCAAACCAG CACCTCAGAA GAAAGAAGTC ATCAAAGAAA AAGTCAAACT TGCTCCTGTA
    AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG GCACTTAAAC CAGAACCCAA AAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323029.1
    CDS338..8326
    Translation

    Target ORF information:

    RefSeq Version XM_021467354.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGTGGC TGAAATTAAG GAGAAAGTTA AACCAGCTTC TATAATGAAA
    GAGCCTGAAG TTGTGAAACA AAAGACTCCT GAACCGAAAC CCAAAAAAGA GCCTGAAGTT
    GTGAAAGAAA AGGCTCCTGA ACCTAAACCC AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA
    GCCAAACCAG CACCTCAGAA GAAAGAAGTC ATCAAAGAAA AAGTCAAACT TGCTCCTGTA
    AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG GCACTTAAAC CAGAACCCAA AAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149691
    Clone ID Related Accession (Same CDS sequence) XM_021467355.1
    Accession Version XM_021467355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323030.1
    CDS338..8326
    Translation

    Target ORF information:

    RefSeq Version XM_021467355.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149692
    Clone ID Related Accession (Same CDS sequence) XM_021467356.1
    Accession Version XM_021467356.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGAGGC TGAAGTAAAG
    GAGAAAGCTA CACCTGCTCC TGTGCACAAA GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA
    CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCT
    AAAAAGACTG AAGTCATCAA AGCGAAAGTA AAACCAGCCC CTGAGAAAAA AGAGGCTGAA
    GTGAAGGAGA AAATTAAACC AGTCCCTGAG AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA
    ATTAAACCTG CTCCTGTAAA GAAAGAGCCT GAAGTTCCTA AAGAAAAGGC TCCTGAACCA
    AAACCCAAAA AAGAACCTGA AGTTATCAAA GAGAAAGTGA AACCAGCGCC AGAGAAGAAA
    GCACCCGAAA TAACCAAAGA AAAACCAAAG CCAGCAGCAA AGAAAGTAGC TGAAGTCATC
    AAAGACAGAA CTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGAAACCA
    AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA GTCATTGAAA AGAAAGTTAT ACCAGCTCCC
    GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA AAGGCTCCTG AACTGACAAC AAAAAAAGCA
    CCTGAGATAA CCAAAGAAAA ACCGAAGCCA GTACCAAAGA AAGAGGCTGA AGTCATTAAA
    GAGAAAGCTA AACTAGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAGGA GAAAGTTAAA
    CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT CATAAGGAAA AAGCACCTGA ACCAAAATCA
    GAACCTACAA AAAAAGTTGA GGTTCCAAAG GAAAAGGCAA AACCAGTTGC TAAAGAAAAG
    GAACCTGAAA TAACCAAAGA AAAGCCAAAA CCAGCACCAA AGAAAGAAGC TGTAGTCATC
    AAAGAAAAAG CTGTACCAGC CATCCTGAAG GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA
    GCTCCTGAGA AGAAAGTGGC TGAAATTAAG GAGAAAGTTA AACCAGCTTC TATAATGAAA
    GAGCCTGAAG TTGTGAAACA AAAGACTCCT GAACCGAAAC CCAAAAAAGA GCCTGAAGTT
    GTGAAAGAAA AGGCTCCTGA ACCTAAACCC AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA
    GCCAAACCAG CACCTCAGAA GAAAGAAGTC ATCAAAGAAA AAGTCAAACT TGCTCCTGTA
    AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG GCACTTAAAC CAGAACCCAA AAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323031.1
    CDS338..8326
    Translation

    Target ORF information:

    RefSeq Version XM_021467356.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGAGGC TGAAGTAAAG
    GAGAAAGCTA CACCTGCTCC TGTGCACAAA GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA
    CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCT
    AAAAAGACTG AAGTCATCAA AGCGAAAGTA AAACCAGCCC CTGAGAAAAA AGAGGCTGAA
    GTGAAGGAGA AAATTAAACC AGTCCCTGAG AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA
    ATTAAACCTG CTCCTGTAAA GAAAGAGCCT GAAGTTCCTA AAGAAAAGGC TCCTGAACCA
    AAACCCAAAA AAGAACCTGA AGTTATCAAA GAGAAAGTGA AACCAGCGCC AGAGAAGAAA
    GCACCCGAAA TAACCAAAGA AAAACCAAAG CCAGCAGCAA AGAAAGTAGC TGAAGTCATC
    AAAGACAGAA CTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGAAACCA
    AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA GTCATTGAAA AGAAAGTTAT ACCAGCTCCC
    GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA AAGGCTCCTG AACTGACAAC AAAAAAAGCA
    CCTGAGATAA CCAAAGAAAA ACCGAAGCCA GTACCAAAGA AAGAGGCTGA AGTCATTAAA
    GAGAAAGCTA AACTAGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAGGA GAAAGTTAAA
    CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT CATAAGGAAA AAGCACCTGA ACCAAAATCA
    GAACCTACAA AAAAAGTTGA GGTTCCAAAG GAAAAGGCAA AACCAGTTGC TAAAGAAAAG
    GAACCTGAAA TAACCAAAGA AAAGCCAAAA CCAGCACCAA AGAAAGAAGC TGTAGTCATC
    AAAGAAAAAG CTGTACCAGC CATCCTGAAG GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA
    GCTCCTGAGA AGAAAGTGGC TGAAATTAAG GAGAAAGTTA AACCAGCTTC TATAATGAAA
    GAGCCTGAAG TTGTGAAACA AAAGACTCCT GAACCGAAAC CCAAAAAAGA GCCTGAAGTT
    GTGAAAGAAA AGGCTCCTGA ACCTAAACCC AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA
    GCCAAACCAG CACCTCAGAA GAAAGAAGTC ATCAAAGAAA AAGTCAAACT TGCTCCTGTA
    AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG GCACTTAAAC CAGAACCCAA AAAAGATGAA
    GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC CTAAACTCCA TCAACCAAAA ACTGTCTCTC
    CTGGACTTGC TAAGGAAGGA CATTGGAGAA ATGAAAAGCG ATCTGGAGTC CACTCAGAAT
    CAAATCACAC TTTTACGGAT GGACAACAGA ACGATCAAAG AGCCAGAGAC TATTGGTGTG
    AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA AAAAGAGACC ACGCTCTGAT CAATGCTACT
    AGGACTGCAC ATATTAAAAC AGAGCGTGAA TCTCTAAAAA ACATCACGAA ACCAGTGCCT
    GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT AAGATTGCGC TGGAGCCTAA AAAGAAAGGA
    ATCATCCCTG AGAAGGTCAA AGAAGTAAAG GAAACTTTAC AGAAAGAACC TGAGTTGGTC
    AAAGATAAAG TAGTTGAGCC AATAGAGAAG GAAATTGTTG TTGAGAAGGA AGTCAAAGGA
    GAAGAGCCTG AAATCACCAA AGATATTCCA GAGGTGGAGG AAGAGGACAT TCCCTACTTC
    CAGTGTTTCT TTGTGGATGA GGATGACACT CAGTATCCGT TCTTCCCTTT CTCTCCACCG
    CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149693
    Clone ID Related Accession (Same CDS sequence) XM_021467357.1
    Accession Version XM_021467357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323032.1
    CDS338..8323
    Translation

    Target ORF information:

    RefSeq Version XM_021467357.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149694
    Clone ID Related Accession (Same CDS sequence) XM_021467358.1
    Accession Version XM_021467358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGATGC TGAAGCTGCT
    AAGGAGAAAG CAAAACCTGC TCCAGTCAAG AAAGGGCCTG AGGTTTCCAG AGAAAAAGCC
    AAACCAGAGC CCGCAAAAAA AGAGCTTGAA GCGGTCAAAG AGAAAGCAAA ACCAGCACCA
    GCTAAAAAAG ATGAAGTCAA AGAGAAAGAA AAACCAGCTC CTGTAAAGAA AGCTGAAGAA
    ATTAAAAAGG CAAAAGCAGC TCCTTCAAGG AAAGAAGCTG TAAAGGAAAA ACCCAAACCT
    GTTTCCGAAA AGAAAGAACC TGCTGTTGCA GTTGAAAAAG CCAAGCCAAA ATCTACAAAG
    AAAGAAGCTG AAGTCGAGGA AAAAGTGAAA CCTGCTCCTG AGAAGAAAGA AGCTGAAGTC
    ATCATGGTGA AAACTAAACC AGTTCTTAAA AAGAAAGAGG CTGAAGTAAT CAAAGAGGTT
    AAACCAGCCC TTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCAGCTCCT
    GTAAAGAAAG AGCCTGAAGT TGTTAAAGAA AAGGAACCTG AACCAAAACC CAAAAGAGAG
    CCAGAAATTA TTAAGGAGAA ATCAGCACCA CCAAAGAAAG TTGAGGTTCC AAAACAAAAG
    GCTCCTGAAA AGAAAGCACC TGAAATAACC AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA
    GAGGCTGAAG CTGTAAAAGA GAAAGCTAAA CCAGAGAAGA AAGAGGCTGA AGCCGTAAAG
    GAGAAAACGA AACCAGCCCC TGAGAAAGAG ACTGAAATAA TCAAGGATAA AATTAAAGCA
    GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG AAGGAAGCAA AACCAGCTCC TGTAAAGAAA
    GAAACTGAAG TCATCAAAGA AAAAGAAAAA CCAGCTCCTG AGAAGAAAGA GGCTGAAGCT
    GTAAAGGAGA AAACGAAACC AACCTCTGAG AAGAAAGAGG CTGAAATAAT TAAGGAGAAA
    GTTAAAGCAG CTCCTGTGAA GAAAGAAGTT GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT
    GTAAAAAAAG AAACTGAAGT CATCAAAGAA AAAGAAAAAA CAGCTCCTGA GAAGAAAGAG
    GCTGAAGCTG TAAAGGAGAA AACGAAACCA ACCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGATAG TTAAACAAGC TCCTGTGAAG AAAGAGGCTG AACCTGTAAA GGAGATAACT
    AAACCAGCCC CTGAGAAGAA AGAGGCTGAA ATAATCAAGG AGAAAGTTAA AGCAGCACCT
    CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC
    ATCAAACAAA AAGCCAAACC AGCTCCTGAG AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA
    GTTAAACCGG CTCCTGTAAA GATAGAGGCT AAACCTGTAA AGGAGATAAC TAAACCAGCC
    CCTGAGAAGA AAGAGGCTGA AATAATCAAG GAGAAGGTCA AATCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGAAA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAG TTAAACCTGC TCCTGTGAGG AAAGAAGCTG AAGAAATAAA GGAAAAAGCT
    GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA GTAGCCAAGG AGATAGTTAA ACCAACTCCT
    TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA AAGGCTCCTG AATCAAAACC CCAAAAAGAG
    GCTGAAATTA TCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAGCC TGAAGTTGCA
    AAAGTACCAA AACCCAAAAA AGAGGCTGAA CTCGCCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AAGAAAAACC TAAGGCAGCA CCAAAGAAAG AGGCTGAAGC TATTAAGGAG
    AAACGTACAC CAGCTCCTGA AAAGAAAGTG GTAGAAGTCA TCAAGGAGAA AGTCAAACCA
    GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG
    CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA CCAAAACCCC AAAAAGAGGT TGAAGTCATC
    AAAGAGAAAG CTAAACCAGC CCTTAAGAAG AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA
    CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT CCCAAAGAAA AGGTGCCTGA ACCAACACCA
    GAACCTGAGA GAGTTGAAGC TCCAAAAGAA AAGGCAAAAG TAATCCCTAG GGAAAAAGTA
    GCTGAGATTA CTAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGTAAAGGAG
    AAAGCTACAC CTGCTCCTGT GCACAAAGAG GTTGAAGTGT TCAAGGAGAA AGTTAAACAA
    GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCTAAA
    AAGACTGAAG TCATCAAAGC GAAAGTAAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323033.1
    CDS338..8323
    Translation

    Target ORF information:

    RefSeq Version XM_021467358.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGATGC TGAAGCTGCT
    AAGGAGAAAG CAAAACCTGC TCCAGTCAAG AAAGGGCCTG AGGTTTCCAG AGAAAAAGCC
    AAACCAGAGC CCGCAAAAAA AGAGCTTGAA GCGGTCAAAG AGAAAGCAAA ACCAGCACCA
    GCTAAAAAAG ATGAAGTCAA AGAGAAAGAA AAACCAGCTC CTGTAAAGAA AGCTGAAGAA
    ATTAAAAAGG CAAAAGCAGC TCCTTCAAGG AAAGAAGCTG TAAAGGAAAA ACCCAAACCT
    GTTTCCGAAA AGAAAGAACC TGCTGTTGCA GTTGAAAAAG CCAAGCCAAA ATCTACAAAG
    AAAGAAGCTG AAGTCGAGGA AAAAGTGAAA CCTGCTCCTG AGAAGAAAGA AGCTGAAGTC
    ATCATGGTGA AAACTAAACC AGTTCTTAAA AAGAAAGAGG CTGAAGTAAT CAAAGAGGTT
    AAACCAGCCC TTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCAGCTCCT
    GTAAAGAAAG AGCCTGAAGT TGTTAAAGAA AAGGAACCTG AACCAAAACC CAAAAGAGAG
    CCAGAAATTA TTAAGGAGAA ATCAGCACCA CCAAAGAAAG TTGAGGTTCC AAAACAAAAG
    GCTCCTGAAA AGAAAGCACC TGAAATAACC AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA
    GAGGCTGAAG CTGTAAAAGA GAAAGCTAAA CCAGAGAAGA AAGAGGCTGA AGCCGTAAAG
    GAGAAAACGA AACCAGCCCC TGAGAAAGAG ACTGAAATAA TCAAGGATAA AATTAAAGCA
    GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG AAGGAAGCAA AACCAGCTCC TGTAAAGAAA
    GAAACTGAAG TCATCAAAGA AAAAGAAAAA CCAGCTCCTG AGAAGAAAGA GGCTGAAGCT
    GTAAAGGAGA AAACGAAACC AACCTCTGAG AAGAAAGAGG CTGAAATAAT TAAGGAGAAA
    GTTAAAGCAG CTCCTGTGAA GAAAGAAGTT GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT
    GTAAAAAAAG AAACTGAAGT CATCAAAGAA AAAGAAAAAA CAGCTCCTGA GAAGAAAGAG
    GCTGAAGCTG TAAAGGAGAA AACGAAACCA ACCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGATAG TTAAACAAGC TCCTGTGAAG AAAGAGGCTG AACCTGTAAA GGAGATAACT
    AAACCAGCCC CTGAGAAGAA AGAGGCTGAA ATAATCAAGG AGAAAGTTAA AGCAGCACCT
    CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC
    ATCAAACAAA AAGCCAAACC AGCTCCTGAG AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA
    GTTAAACCGG CTCCTGTAAA GATAGAGGCT AAACCTGTAA AGGAGATAAC TAAACCAGCC
    CCTGAGAAGA AAGAGGCTGA AATAATCAAG GAGAAGGTCA AATCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGAAA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAG TTAAACCTGC TCCTGTGAGG AAAGAAGCTG AAGAAATAAA GGAAAAAGCT
    GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA GTAGCCAAGG AGATAGTTAA ACCAACTCCT
    TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA AAGGCTCCTG AATCAAAACC CCAAAAAGAG
    GCTGAAATTA TCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAGCC TGAAGTTGCA
    AAAGTACCAA AACCCAAAAA AGAGGCTGAA CTCGCCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AAGAAAAACC TAAGGCAGCA CCAAAGAAAG AGGCTGAAGC TATTAAGGAG
    AAACGTACAC CAGCTCCTGA AAAGAAAGTG GTAGAAGTCA TCAAGGAGAA AGTCAAACCA
    GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG
    CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA CCAAAACCCC AAAAAGAGGT TGAAGTCATC
    AAAGAGAAAG CTAAACCAGC CCTTAAGAAG AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA
    CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT CCCAAAGAAA AGGTGCCTGA ACCAACACCA
    GAACCTGAGA GAGTTGAAGC TCCAAAAGAA AAGGCAAAAG TAATCCCTAG GGAAAAAGTA
    GCTGAGATTA CTAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGTAAAGGAG
    AAAGCTACAC CTGCTCCTGT GCACAAAGAG GTTGAAGTGT TCAAGGAGAA AGTTAAACAA
    GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCTAAA
    AAGACTGAAG TCATCAAAGC GAAAGTAAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149695
    Clone ID Related Accession (Same CDS sequence) XM_021467359.1
    Accession Version XM_021467359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAG
    GCTGAAGCTG TAAAGGAGAA AACGAAACCA ACCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGATAG TTAAACAAGC TCCTGTGAAG AAAGAGGCTG AACCTGTAAA GGAGATAACT
    AAACCAGCCC CTGAGAAGAA AGAGGCTGAA ATAATCAAGG AGAAAGTTAA AGCAGCACCT
    CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC
    ATCAAACAAA AAGCCAAACC AGCTCCTGAG AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA
    GTTAAACCGG CTCCTGTAAA GATAGAGGCT AAACCTGTAA AGGAGATAAC TAAACCAGCC
    CCTGAGAAGA AAGAGGCTGA AATAATCAAG GAGAAGGTCA AATCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGAAA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAG TTAAACCTGC TCCTGTGAGG AAAGAAGCTG AAGAAATAAA GGAAAAAGCT
    GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA GTAGCCAAGG AGATAGTTAA ACCAACTCCT
    TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA AAGGCTCCTG AATCAAAACC CCAAAAAGAG
    GCTGAAATTA TCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAGCC TGAAGTTGCA
    AAAGTACCAA AACCCAAAAA AGAGGCTGAA CTCGCCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AAGAAAAACC TAAGGCAGCA CCAAAGAAAG AGGCTGAAGC TATTAAGGAG
    AAACGTACAC CAGCTCCTGA AAAGAAAGTG GTAGAAGTCA TCAAGGAGAA AGTCAAACCA
    GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG
    CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA CCAAAACCCC AAAAAGAGGT TGAAGTCATC
    AAAGAGAAAG CTAAACCAGC CCTTAAGAAG AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA
    CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT CCCAAAGAAA AGGTGCCTGA ACCAACACCA
    GAACCTGAGA GAGTTGAAGC TCCAAAAGAA AAGGCAAAAG TAATCCCTAG GGAAAAAGTA
    GCTGAGATTA CTAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGTAAAGGAG
    AAAGCTACAC CTGCTCCTGT GCACAAAGAG GTTGAAGTGT TCAAGGAGAA AGTTAAACAA
    GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCTAAA
    AAGACTGAAG TCATCAAAGC GAAAGTAAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323034.1
    CDS338..8323
    Translation

    Target ORF information:

    RefSeq Version XM_021467359.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAG
    GCTGAAGCTG TAAAGGAGAA AACGAAACCA ACCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGATAG TTAAACAAGC TCCTGTGAAG AAAGAGGCTG AACCTGTAAA GGAGATAACT
    AAACCAGCCC CTGAGAAGAA AGAGGCTGAA ATAATCAAGG AGAAAGTTAA AGCAGCACCT
    CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC
    ATCAAACAAA AAGCCAAACC AGCTCCTGAG AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA
    GTTAAACCGG CTCCTGTAAA GATAGAGGCT AAACCTGTAA AGGAGATAAC TAAACCAGCC
    CCTGAGAAGA AAGAGGCTGA AATAATCAAG GAGAAGGTCA AATCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGAAA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAG TTAAACCTGC TCCTGTGAGG AAAGAAGCTG AAGAAATAAA GGAAAAAGCT
    GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA GTAGCCAAGG AGATAGTTAA ACCAACTCCT
    TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA AAGGCTCCTG AATCAAAACC CCAAAAAGAG
    GCTGAAATTA TCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAGCC TGAAGTTGCA
    AAAGTACCAA AACCCAAAAA AGAGGCTGAA CTCGCCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AAGAAAAACC TAAGGCAGCA CCAAAGAAAG AGGCTGAAGC TATTAAGGAG
    AAACGTACAC CAGCTCCTGA AAAGAAAGTG GTAGAAGTCA TCAAGGAGAA AGTCAAACCA
    GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG
    CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA CCAAAACCCC AAAAAGAGGT TGAAGTCATC
    AAAGAGAAAG CTAAACCAGC CCTTAAGAAG AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA
    CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT CCCAAAGAAA AGGTGCCTGA ACCAACACCA
    GAACCTGAGA GAGTTGAAGC TCCAAAAGAA AAGGCAAAAG TAATCCCTAG GGAAAAAGTA
    GCTGAGATTA CTAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGTAAAGGAG
    AAAGCTACAC CTGCTCCTGT GCACAAAGAG GTTGAAGTGT TCAAGGAGAA AGTTAAACAA
    GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCTAAA
    AAGACTGAAG TCATCAAAGC GAAAGTAAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149696
    Clone ID Related Accession (Same CDS sequence) XM_021467360.1
    Accession Version XM_021467360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA GGCTGAAGCT
    GTAAAGGAGA AAACGAAACC AACCTCTGAG AAGAAAGAGG CTGAAATAAT TAAGGAGAAA
    GTTAAAGCAG CTCCTGTGAA GAAAGAAGTT GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT
    GTAAAAAAAG AAACTGAAGT CATCAAAGAA AAAGAAAAAA CAGCTCCTGA GAAGAAAGAG
    GCTGAAGCTG TAAAGGAGAA AACGAAACCA ACCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGATAG TTAAACAAGC TCCTGTGAAG AAAGAGGCTG AACCTGTAAA GGAGATAACT
    AAACCAGCCC CTGAGAAGAA AGAGGCTGAA ATAATCAAGG AGAAAGTTAA AGCAGCACCT
    CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC
    ATCAAACAAA AAGCCAAACC AGCTCCTGAG AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA
    GTTAAACCGG CTCCTGTAAA GATAGAGGCT AAACCTGTAA AGGAGATAAC TAAACCAGCC
    CCTGAGAAGA AAGAGGCTGA AATAATCAAG GAGAAGGTCA AATCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGAAA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAG TTAAACCTGC TCCTGTGAGG AAAGAAGCTG AAGAAATAAA GGAAAAAGCT
    GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA GTAGCCAAGG AGATAGTTAA ACCAACTCCT
    TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA AAGGCTCCTG AATCAAAACC CCAAAAAGAG
    GCTGAAATTA TCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAGCC TGAAGTTGCA
    AAAGTACCAA AACCCAAAAA AGAGGCTGAA CTCGCCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AAGAAAAACC TAAGGCAGCA CCAAAGAAAG AGGCTGAAGC TATTAAGGAG
    AAACGTACAC CAGCTCCTGA AAAGAAAGTG GTAGAAGTCA TCAAGGAGAA AGTCAAACCA
    GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG
    CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA CCAAAACCCC AAAAAGAGGT TGAAGTCATC
    AAAGAGAAAG CTAAACCAGC CCTTAAGAAG AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA
    CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT CCCAAAGAAA AGGTGCCTGA ACCAACACCA
    GAACCTGAGA GAGTTGAAGC TCCAAAAGAA AAGGCAAAAG TAATCCCTAG GGAAAAAGTA
    GCTGAGATTA CTAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGTAAAGGAG
    AAAGCTACAC CTGCTCCTGT GCACAAAGAG GTTGAAGTGT TCAAGGAGAA AGTTAAACAA
    GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCTAAA
    AAGACTGAAG TCATCAAAGC GAAAGTAAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323035.1
    CDS338..8323
    Translation

    Target ORF information:

    RefSeq Version XM_021467360.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA GGCTGAAGCT
    GTAAAGGAGA AAACGAAACC AACCTCTGAG AAGAAAGAGG CTGAAATAAT TAAGGAGAAA
    GTTAAAGCAG CTCCTGTGAA GAAAGAAGTT GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT
    GTAAAAAAAG AAACTGAAGT CATCAAAGAA AAAGAAAAAA CAGCTCCTGA GAAGAAAGAG
    GCTGAAGCTG TAAAGGAGAA AACGAAACCA ACCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGATAG TTAAACAAGC TCCTGTGAAG AAAGAGGCTG AACCTGTAAA GGAGATAACT
    AAACCAGCCC CTGAGAAGAA AGAGGCTGAA ATAATCAAGG AGAAAGTTAA AGCAGCACCT
    CTGAAGAAAG AAGTCAAGGA GAAAGCAAAA CCAGCTCCTG TAAAGAAAGA AGCTGAAGTC
    ATCAAACAAA AAGCCAAACC AGCTCCTGAG AAGAAAGAGG CCAAAGTCAT CAAGGAGAAA
    GTTAAACCGG CTCCTGTAAA GATAGAGGCT AAACCTGTAA AGGAGATAAC TAAACCAGCC
    CCTGAGAAGA AAGAGGCTGA AATAATCAAG GAGAAGGTCA AATCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGAAA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAG TTAAACCTGC TCCTGTGAGG AAAGAAGCTG AAGAAATAAA GGAAAAAGCT
    GAAGCAGTTT ATGAGAAAAA AGAGGCTGAA GTAGCCAAGG AGATAGTTAA ACCAACTCCT
    TTAAAGAAGG AGCCCGAAGT TGTGAAGGAA AAGGCTCCTG AATCAAAACC CCAAAAAGAG
    GCTGAAATTA TCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAGCC TGAAGTTGCA
    AAAGTACCAA AACCCAAAAA AGAGGCTGAA CTCGCCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AAGAAAAACC TAAGGCAGCA CCAAAGAAAG AGGCTGAAGC TATTAAGGAG
    AAACGTACAC CAGCTCCTGA AAAGAAAGTG GTAGAAGTCA TCAAGGAGAA AGTCAAACCA
    GCCCCTGAGA AAAAAGCGGT TCTCAAGGAG AAAGTTAGAC CTGCTCCTGA AAAGAAAGAG
    CCTGAAGTTG CTAAAGAAAA GGCTCCTGAA CCAAAACCCC AAAAAGAGGT TGAAGTCATC
    AAAGAGAAAG CTAAACCAGC CCTTAAGAAG AAAGAGCCTA AAGTCAAGGA AAAGGTTCAA
    CCAGCTCCTA TAAAGAAAGA GCCAGAGCTT CCCAAAGAAA AGGTGCCTGA ACCAACACCA
    GAACCTGAGA GAGTTGAAGC TCCAAAAGAA AAGGCAAAAG TAATCCCTAG GGAAAAAGTA
    GCTGAGATTA CTAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGTAAAGGAG
    AAAGCTACAC CTGCTCCTGT GCACAAAGAG GTTGAAGTGT TCAAGGAGAA AGTTAAACAA
    GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCTAAA
    AAGACTGAAG TCATCAAAGC GAAAGTAAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149697
    Clone ID Related Accession (Same CDS sequence) XM_021467361.1
    Accession Version XM_021467361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGCCA
    GAAATTATTA AGGAGAAATC AGCACCACCA AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT
    CCTGAAAAGA AAGCACCTGA AATAACCAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGCTG TAAAAGAGAA AGCTAAACCA GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG
    AAAACGAAAC CAGCCCCTGA GAAAGAGACT GAAATAATCA AGGATAAAAT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAACCA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CTCTGAGAAG AAAGAGGCTG AAATAATTAA GGAGAAAGTT
    AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA GTCAAGGAGG AAGCAAAACC AGCTCCTGTA
    AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA GAAAAAACAG CTCCTGAGAA GAAAGAGGCT
    GAAGCTGTAA AGGAGAAAAC GAAACCAACC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGATAGTTA AACAAGCTCC TGTGAAGAAA GAGGCTGAAC CTGTAAAGGA GATAACTAAA
    CCAGCCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323036.1
    CDS338..8320
    Translation

    Target ORF information:

    RefSeq Version XM_021467361.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGCCA
    GAAATTATTA AGGAGAAATC AGCACCACCA AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT
    CCTGAAAAGA AAGCACCTGA AATAACCAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGCTG TAAAAGAGAA AGCTAAACCA GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG
    AAAACGAAAC CAGCCCCTGA GAAAGAGACT GAAATAATCA AGGATAAAAT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAACCA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CTCTGAGAAG AAAGAGGCTG AAATAATTAA GGAGAAAGTT
    AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA GTCAAGGAGG AAGCAAAACC AGCTCCTGTA
    AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA GAAAAAACAG CTCCTGAGAA GAAAGAGGCT
    GAAGCTGTAA AGGAGAAAAC GAAACCAACC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGATAGTTA AACAAGCTCC TGTGAAGAAA GAGGCTGAAC CTGTAAAGGA GATAACTAAA
    CCAGCCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149698
    Clone ID Related Accession (Same CDS sequence) XM_021467362.1
    Accession Version XM_021467362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323037.1
    CDS338..8320
    Translation

    Target ORF information:

    RefSeq Version XM_021467362.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149699
    Clone ID Related Accession (Same CDS sequence) XM_021467363.1
    Accession Version XM_021467363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323038.1
    CDS338..8320
    Translation

    Target ORF information:

    RefSeq Version XM_021467363.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149700
    Clone ID Related Accession (Same CDS sequence) XM_021467364.1
    Accession Version XM_021467364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323039.1
    CDS338..8320
    Translation

    Target ORF information:

    RefSeq Version XM_021467364.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149701
    Clone ID Related Accession (Same CDS sequence) XM_021467365.1
    Accession Version XM_021467365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGGGGA TGCAGACATC
    ACTGAAACAG CCAAACTTGG AGGCACTGTA TTGGATACAG TTACTGACTG GATTCGATTG
    CCAGTGGGAT ACTTATTTTA TTTATTTGAA GCCATCCTGG AGGTAACCAT TATTTATCCA
    GCAGATATGG CAAGTGAAGC ATTTTTGCAA ATTTTAAATG TGATCAAGAA CACTTCATCA
    GCCATATTTG GGTTTCTTGA AGCTTGGTTC CCCAAAATGA GCACTCGTCC TACAGAGCTG
    GTTGAAAAGG TTGCAGAGGA GGCCACTGAT GTAAAGACCA CCGTCTCTAA TTATCTGTCC
    AGTCTGTTTG TGAGGGATGA AGGTGGTATT CCTGACGTCA CTTTTGATCC CATGAAAGTT
    GTCTCAGACA CCGTTGAGGA GTTTGCAGAC AGAAGAGATA TGTTATTAGC TTATCTCTCA
    AACATGCTCA TGGAAGACAA AGTTTCAGAT GAAACACCAC AGGTGATAAG GCGAAAAGGA
    GAATTTCTGC CCCCTATGGA AAAAGTTGCA GAAGTACTAG GAAAAACTCA GGACCCCATC
    TACGCTGCCT ACATCGCTGT GAGATCCACA GCTGAGACAG ACGGCGATGA ACTGGAAATA
    AAGGGGGCTG AGGTGGACTC TGCTGAAGAT ATCTTGGAAG AACAAAAAGT TGAAAAGACA
    GTGGACGGCT CTGAAAATGA TGCAGTTGCC TCTGTTGAGG AGGAAAAAGC AGTTGTTGAT
    ATTGTAGATG AGGAATCTTC TCATACGCCT GAAGTCACAG ATGAAGAAAT GGCAAAAGAA
    AAAGATGAAG ACACTTCTGA TTCAACTGAA ATGACTTCTG ATGTGCAAGA CATTGATGAT
    ACCATAGATT CTGAGACTGA AGAAATTCCT GAAATGGTTG AAAGTGTTTC AGAGGTTGGA
    GAAGAGGAAC CTTTTGTAAT TGAAAAGGTT GAAGACACCT CTGATTCGAA AGAAATCACT
    TCTAATGCCG CAGATGTGCA AGATGTTGAT GATAATTCTG ATGTTGATAC TGAAGAAATT
    CCTGAATTGG TTGGAGGTGT TTCAGATGTT GGTGAAGAGG AACCTTCTGA AGTCATAGAT
    GAAGAAATTG CAACTGAAAA GCTTGAAGAC GCCTCTGATT CAACTGGATT GATTCCCAGT
    GCTGAAAATG TGAAAGACAC TGACGATATC TTAGAAGTTG ACGCAGAGGT GAATGAAGTT
    GAAACAGTTG AAGACATTTC AGAAGTTACA AATGAGGAAA CTTCTGATGT GCCAGAAGTC
    ATAGATGAGG AAAAGGTTGA AAAGTCTGAT GATATCTTTC ATTTTGCAGA GGACAAACCA
    ACTGAATTGA TTTCTGATGC TCCAGATGTA ACAGAAGACA TTGAAATCTC AATCTCTGCA
    GTTTCAGAGG ATAATGAAAC AATTCAAGAA GCTGATATGA CAGATGTCAA AGATGAGGAA
    ATGGAAGCTG AAAAGGTTGA TGAAATCTCC TATTTTGTAG AGGATAAATC AACTGAAATT
    ACTTCTGATG CTACAGATGT GAAAGAAGAC ACTGATGGCT TTACAGTTAC AGAGGAGAAA
    GGAACAATTG AAAGCCTTCA AGAAGCAGCA ATGAAAATGT CGGGAAATGC AGATGATGAA
    ATCCCCAATG TCACAGAGAT GGTTGAAGAA CCTGATACTT CTGATGTTAT GGATGAGCTA
    GACGAACCTG AAAAAGATGA AGATGTAATC AGTGTCACCA ATGAAAGGGT TACAACTGCA
    CAAGATGTCA TATTTTCAAA GGAAAAAATG GGAGCAGCAG AACTGGATGA TCATGACAAA
    CTCAGGGCTA AGGATGATTC AGAAGTGGAT CACATGGAGA AGGAAATTGC AGATGCTGAA
    AATGACCTTC AGAATGAGAT AGATAAAGAT GAGTTTAAGG TTGAAGATGC ATCTCATCAA
    ACTATTTTGG GAAGCACTGC TGCTGAAGAA GACCAAGATG ATGATGATGA TGATGAAGAA
    CCTGTCCTTC TTACTGTCAA ACATGAGCTC ACAGACGGTG ATGATGAAAA TAATAATAAC
    AACGACAGAA AGAGAGATGC CAGAACTAAA GGTCTGATCA ATAGAAAAAC ACCAAAGAGC
    CATTCCACTG AAACTGATGA ACAAAAGGCG CACAAGGATC TTCATAAAGA GTCTGCTAAA
    GTTCTTGAGC AAGTGAAAGA AGCCAAAGAG AAAAAAGCTA GAGAGGAGAT ATACAAAATC
    ATTCAAGAAA TGAGGGAGGA GAAGGCTGAA GGTGTGGAGG AAGAGATCGT GTTACAACAG
    ATAAACATAA CAGTAGAAAA GGTCAAGAAA GAAGAAACTG TCAAAAAGGA GGAAAAGGCT
    AAAAGAAAAG AAAAGGCTCA TAAACCAAAG GATGCAAAGA TTGCCAAAGA CAAACCTAAA
    GCAGTCCCAG TAAAGCAAGA GGATGAAGTA GCCAAGAAAG AAACCAAACC AGCACCATCT
    AAGAAAGAGG CTAAAATTGT GAAGGAGAAA CCCAAAGCAC ATCCTGCAAA GAAAGAAAAA
    GCTAAAGATA TTAAGGAAAA GGCAAAATCT GGTCAAAAAG AAAAAGTTGC TGAGGCTGTC
    AAGGAAAAAT CGAAGCCAGT AAAGAAAGAA GCTGAAAAAA TTGATGCCAA AGTCAAACCA
    GCAGCAGAAA AGAAAGTGGA TGAAGCCCCC AAAGAGAAAC CTAAAGTGAA GAAAGAGGCT
    GAAGTAATCA AAAAGATTAA ACTAGAGCCT GAGAGGAAAG AAGCTAAAAC TCCCAAGGCC
    AAAATTGAAC CAGCTGGTTT GAAGAAAGAT GCTGAAGCTG CTAAGGAGAA AGCAAAACCT
    GCTCCAGTCA AGAAAGGGCC TGAGGTTTCC AGAGAAAAAG CCAAACCAGA GCCCGCAAAA
    AAAGAGCTTG AAGCGGTCAA AGAGAAAGCA AAACCAGCAC CAGCTAAAAA AGATGAAGTC
    AAAGAGAAAG AAAAACCAGC TCCTGTAAAG AAAGCTGAAG AAATTAAAAA GGCAAAAGCA
    GCTCCTTCAA GGAAAGAAGC TGTAAAGGAA AAACCCAAAC CTGTTTCCGA AAAGAAAGAA
    CCTGCTGTTG CAGTTGAAAA AGCCAAGCCA AAATCTACAA AGAAAGAAGC TGAAGTCGAG
    GAAAAAGTGA AACCTGCTCC TGAGAAGAAA GAAGCTGAAG TCATCATGGT GAAAACTAAA
    CCAGTTCTTA AAAAGAAAGA GGCTGAAGTA ATCAAAGAGG TTAAACCAGC CCTTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAAGTT AAACCAGCTC CTGTAAAGAA AGAGCCTGAA
    GTTGTTAAAG AAAAGGAACC TGAACCAAAA CCCAAAAGAG AGCCAGAAAT TATTAAGGAG
    AAATCAGCAC CACCAAAGAA AGTTGAGGTT CCAAAACAAA AGGCTCCTGA AAAGAAAGCA
    CCTGAAATAA CCAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGCTGTAAAA
    GAGAAAGCTA AACCAGAGAA GAAAGAGGCT GAAGCCGTAA AGGAGAAAAC GAAACCAGCC
    CCTGAGAAAG AGACTGAAAT AATCAAGGAT AAAATTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGAAGGAAGC AAAACCAGCT CCTGTAAAGA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AACCAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCTCTG AGAAGAAAGA GGCTGAAATA ATTAAGGAGA AAGTTAAAGC AGCTCCTGTG
    AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA AAACCAGCTC CTGTAAAAAA AGAAACTGAA
    GTCATCAAAG AAAAAGAAAA AACAGCTCCT GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG
    AAAACGAAAC CAACCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAT AGTTAAACAA
    GCTCCTGTGA AGAAAGAGGC TGAACCTGTA AAGGAGATAA CTAAACCAGC CCCTGAGAAG
    AAAGAGGCTG AAATAATCAA GGAGAAAGTT AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG
    GAGAAAGCAA AACCAGCTCC TGTAAAGAAA GAAGCTGAAG TCATCAAACA AAAAGCCAAA
    CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC ATCAAGGAGA AAGTTAAACC GGCTCCTGTA
    AAGATAGAGG CTAAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAGGT CAAATCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG
    GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG GCTGAAGTCA TCAAGGAGAA AGTTAAACCT
    GCTCCTGTGA GGAAAGAAGC TGAAGAAATA AAGGAAAAAG CTGAAGCAGT TTATGAGAAA
    AAAGAGGCTG AAGTAGCCAA GGAGATAGTT AAACCAACTC CTTTAAAGAA GGAGCCCGAA
    GTTGTGAAGG AAAAGGCTCC TGAATCAAAA CCCCAAAAAG AGGCTGAAAT TATCAAAGAG
    AAAGTTAAAC CTGCACCTGT AAAGAAAGAG CCTGAAGTTG CAAAAGTACC AAAACCCAAA
    AAAGAGGCTG AACTCGCCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAAGAAAAA
    CCTAAGGCAG CACCAAAGAA AGAGGCTGAA GCTATTAAGG AGAAACGTAC ACCAGCTCCT
    GAAAAGAAAG TGGTAGAAGT CATCAAGGAG AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG
    GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA
    AAGGCTCCTG AACCAAAACC CCAAAAAGAG GTTGAAGTCA TCAAAGAGAA AGCTAAACCA
    GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG GAAAAGGTTC AACCAGCTCC TATAAAGAAA
    GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT GAACCAACAC CAGAACCTGA GAGAGTTGAA
    GCTCCAAAAG AAAAGGCAAA AGTAATCCCT AGGGAAAAAG TAGCTGAGAT TACTAAAGAA
    AAACCAAAGC CAGCACCAAA GAAAGAGGCT GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT
    GTGCACAAAG AGGTTGAAGT GTTCAAGGAG AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG
    CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA CCAAAACCTA AAAAGACTGA AGTCATCAAA
    GCGAAAGTAA AACCAGCCCC TGAGAAAAAA GAGGCTGAAG TGAAGGAGAA AATTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATA AAGGAGAGAA TTAAACCTGC TCCTGTAAAG
    AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCCAAAAA AGAACCTGAA
    GTTATCAAAG AGAAAGTGAA ACCAGCGCCA GAGAAGAAAG CACCCGAAAT AACCAAAGAA
    AAACCAAAGC CAGCAGCAAA GAAAGTAGCT GAAGTCATCA AAGACAGAAC TAAACCAGTC
    CCTGAGAAGA AAGAGGCTGA AGTCATCAAG GAGAAACCAA AAGCAGTTCC CGAGAAGAAA
    GAGGCTAAAG TCATTGAAAA GAAAGTTATA CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT
    GTTAAGGAAA AGGCTCCTGA ACTGACAACA AAAAAAGCAC CTGAGATAAC CAAAGAAAAA
    CCGAAGCCAG TACCAAAGAA AGAGGCTGAA GTCATTAAAG AGAAAGCTAA ACTAGCTCCT
    GAAAAGAAAG AGGTTGAAGT CATCAAGGAG AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG
    CCTGAGGTTC ATAAGGAAAA AGCACCTGAA CCAAAATCAG AACCTACAAA AAAAGTTGAG
    GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT AAAGAAAAGG AACCTGAAAT AACCAAAGAA
    AAGCCAAAAC CAGCACCAAA GAAAGAAGCT GTAGTCATCA AAGAAAAAGC TGTACCAGCC
    ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT
    GAAATTAAGG AGAAAGTTAA ACCAGCTTCT ATAATGAAAG AGCCTGAAGT TGTGAAACAA
    AAGACTCCTG AACCGAAACC CAAAAAAGAG CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA
    CCTAAACCCA AAAAAGAGCC TGAAGTTGCT AAAGAGAAAG CCAAACCAGC ACCTCAGAAG
    AAAGAAGTCA TCAAAGAAAA AGTCAAACTT GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC
    GAAGAAAAGG CACTTAAACC AGAACCCAAA AAAGATGAAG TTGGTCTTAT TGGTCTGGAC
    CTGGAGCTCC TAAACTCCAT CAACCAAAAA CTGTCTCTCC TGGACTTGCT AAGGAAGGAC
    ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC ACTCAGAATC AAATCACACT TTTACGGATG
    GACAACAGAA CGATCAAAGA GCCAGAGACT ATTGGTGTGA AAGTAAAACC AGCAGTCAAG
    GAAAAAGCAA AAAGAGACCA CGCTCTGATC AATGCTACTA GGACTGCACA TATTAAAACA
    GAGCGTGAAT CTCTAAAAAA CATCACGAAA CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT
    CCAAAGGTTA AGATTGCGCT GGAGCCTAAA AAGAAAGGAA TCATCCCTGA GAAGGTCAAA
    GAAGTAAAGG AAACTTTACA GAAAGAACCT GAGTTGGTCA AAGATAAAGT AGTTGAGCCA
    ATAGAGAAGG AAATTGTTGT TGAGAAGGAA GTCAAAGGAG AAGAGCCTGA AATCACCAAA
    GATATTCCAG AGGTGGAGGA AGAGGACATT CCCTACTTCC AGTGTTTCTT TGTGGATGAG
    GATGACACTC AGTATCCGTT CTTCCCTTTC TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323040.1
    CDS268..8235
    Translation

    Target ORF information:

    RefSeq Version XM_021467365.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGGGGA TGCAGACATC
    ACTGAAACAG CCAAACTTGG AGGCACTGTA TTGGATACAG TTACTGACTG GATTCGATTG
    CCAGTGGGAT ACTTATTTTA TTTATTTGAA GCCATCCTGG AGGTAACCAT TATTTATCCA
    GCAGATATGG CAAGTGAAGC ATTTTTGCAA ATTTTAAATG TGATCAAGAA CACTTCATCA
    GCCATATTTG GGTTTCTTGA AGCTTGGTTC CCCAAAATGA GCACTCGTCC TACAGAGCTG
    GTTGAAAAGG TTGCAGAGGA GGCCACTGAT GTAAAGACCA CCGTCTCTAA TTATCTGTCC
    AGTCTGTTTG TGAGGGATGA AGGTGGTATT CCTGACGTCA CTTTTGATCC CATGAAAGTT
    GTCTCAGACA CCGTTGAGGA GTTTGCAGAC AGAAGAGATA TGTTATTAGC TTATCTCTCA
    AACATGCTCA TGGAAGACAA AGTTTCAGAT GAAACACCAC AGGTGATAAG GCGAAAAGGA
    GAATTTCTGC CCCCTATGGA AAAAGTTGCA GAAGTACTAG GAAAAACTCA GGACCCCATC
    TACGCTGCCT ACATCGCTGT GAGATCCACA GCTGAGACAG ACGGCGATGA ACTGGAAATA
    AAGGGGGCTG AGGTGGACTC TGCTGAAGAT ATCTTGGAAG AACAAAAAGT TGAAAAGACA
    GTGGACGGCT CTGAAAATGA TGCAGTTGCC TCTGTTGAGG AGGAAAAAGC AGTTGTTGAT
    ATTGTAGATG AGGAATCTTC TCATACGCCT GAAGTCACAG ATGAAGAAAT GGCAAAAGAA
    AAAGATGAAG ACACTTCTGA TTCAACTGAA ATGACTTCTG ATGTGCAAGA CATTGATGAT
    ACCATAGATT CTGAGACTGA AGAAATTCCT GAAATGGTTG AAAGTGTTTC AGAGGTTGGA
    GAAGAGGAAC CTTTTGTAAT TGAAAAGGTT GAAGACACCT CTGATTCGAA AGAAATCACT
    TCTAATGCCG CAGATGTGCA AGATGTTGAT GATAATTCTG ATGTTGATAC TGAAGAAATT
    CCTGAATTGG TTGGAGGTGT TTCAGATGTT GGTGAAGAGG AACCTTCTGA AGTCATAGAT
    GAAGAAATTG CAACTGAAAA GCTTGAAGAC GCCTCTGATT CAACTGGATT GATTCCCAGT
    GCTGAAAATG TGAAAGACAC TGACGATATC TTAGAAGTTG ACGCAGAGGT GAATGAAGTT
    GAAACAGTTG AAGACATTTC AGAAGTTACA AATGAGGAAA CTTCTGATGT GCCAGAAGTC
    ATAGATGAGG AAAAGGTTGA AAAGTCTGAT GATATCTTTC ATTTTGCAGA GGACAAACCA
    ACTGAATTGA TTTCTGATGC TCCAGATGTA ACAGAAGACA TTGAAATCTC AATCTCTGCA
    GTTTCAGAGG ATAATGAAAC AATTCAAGAA GCTGATATGA CAGATGTCAA AGATGAGGAA
    ATGGAAGCTG AAAAGGTTGA TGAAATCTCC TATTTTGTAG AGGATAAATC AACTGAAATT
    ACTTCTGATG CTACAGATGT GAAAGAAGAC ACTGATGGCT TTACAGTTAC AGAGGAGAAA
    GGAACAATTG AAAGCCTTCA AGAAGCAGCA ATGAAAATGT CGGGAAATGC AGATGATGAA
    ATCCCCAATG TCACAGAGAT GGTTGAAGAA CCTGATACTT CTGATGTTAT GGATGAGCTA
    GACGAACCTG AAAAAGATGA AGATGTAATC AGTGTCACCA ATGAAAGGGT TACAACTGCA
    CAAGATGTCA TATTTTCAAA GGAAAAAATG GGAGCAGCAG AACTGGATGA TCATGACAAA
    CTCAGGGCTA AGGATGATTC AGAAGTGGAT CACATGGAGA AGGAAATTGC AGATGCTGAA
    AATGACCTTC AGAATGAGAT AGATAAAGAT GAGTTTAAGG TTGAAGATGC ATCTCATCAA
    ACTATTTTGG GAAGCACTGC TGCTGAAGAA GACCAAGATG ATGATGATGA TGATGAAGAA
    CCTGTCCTTC TTACTGTCAA ACATGAGCTC ACAGACGGTG ATGATGAAAA TAATAATAAC
    AACGACAGAA AGAGAGATGC CAGAACTAAA GGTCTGATCA ATAGAAAAAC ACCAAAGAGC
    CATTCCACTG AAACTGATGA ACAAAAGGCG CACAAGGATC TTCATAAAGA GTCTGCTAAA
    GTTCTTGAGC AAGTGAAAGA AGCCAAAGAG AAAAAAGCTA GAGAGGAGAT ATACAAAATC
    ATTCAAGAAA TGAGGGAGGA GAAGGCTGAA GGTGTGGAGG AAGAGATCGT GTTACAACAG
    ATAAACATAA CAGTAGAAAA GGTCAAGAAA GAAGAAACTG TCAAAAAGGA GGAAAAGGCT
    AAAAGAAAAG AAAAGGCTCA TAAACCAAAG GATGCAAAGA TTGCCAAAGA CAAACCTAAA
    GCAGTCCCAG TAAAGCAAGA GGATGAAGTA GCCAAGAAAG AAACCAAACC AGCACCATCT
    AAGAAAGAGG CTAAAATTGT GAAGGAGAAA CCCAAAGCAC ATCCTGCAAA GAAAGAAAAA
    GCTAAAGATA TTAAGGAAAA GGCAAAATCT GGTCAAAAAG AAAAAGTTGC TGAGGCTGTC
    AAGGAAAAAT CGAAGCCAGT AAAGAAAGAA GCTGAAAAAA TTGATGCCAA AGTCAAACCA
    GCAGCAGAAA AGAAAGTGGA TGAAGCCCCC AAAGAGAAAC CTAAAGTGAA GAAAGAGGCT
    GAAGTAATCA AAAAGATTAA ACTAGAGCCT GAGAGGAAAG AAGCTAAAAC TCCCAAGGCC
    AAAATTGAAC CAGCTGGTTT GAAGAAAGAT GCTGAAGCTG CTAAGGAGAA AGCAAAACCT
    GCTCCAGTCA AGAAAGGGCC TGAGGTTTCC AGAGAAAAAG CCAAACCAGA GCCCGCAAAA
    AAAGAGCTTG AAGCGGTCAA AGAGAAAGCA AAACCAGCAC CAGCTAAAAA AGATGAAGTC
    AAAGAGAAAG AAAAACCAGC TCCTGTAAAG AAAGCTGAAG AAATTAAAAA GGCAAAAGCA
    GCTCCTTCAA GGAAAGAAGC TGTAAAGGAA AAACCCAAAC CTGTTTCCGA AAAGAAAGAA
    CCTGCTGTTG CAGTTGAAAA AGCCAAGCCA AAATCTACAA AGAAAGAAGC TGAAGTCGAG
    GAAAAAGTGA AACCTGCTCC TGAGAAGAAA GAAGCTGAAG TCATCATGGT GAAAACTAAA
    CCAGTTCTTA AAAAGAAAGA GGCTGAAGTA ATCAAAGAGG TTAAACCAGC CCTTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAAGTT AAACCAGCTC CTGTAAAGAA AGAGCCTGAA
    GTTGTTAAAG AAAAGGAACC TGAACCAAAA CCCAAAAGAG AGCCAGAAAT TATTAAGGAG
    AAATCAGCAC CACCAAAGAA AGTTGAGGTT CCAAAACAAA AGGCTCCTGA AAAGAAAGCA
    CCTGAAATAA CCAAAGAAAA ACCAAAGCCA GCACCAAAGA AAGAGGCTGA AGCTGTAAAA
    GAGAAAGCTA AACCAGAGAA GAAAGAGGCT GAAGCCGTAA AGGAGAAAAC GAAACCAGCC
    CCTGAGAAAG AGACTGAAAT AATCAAGGAT AAAATTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGAAGGAAGC AAAACCAGCT CCTGTAAAGA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AACCAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCTCTG AGAAGAAAGA GGCTGAAATA ATTAAGGAGA AAGTTAAAGC AGCTCCTGTG
    AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA AAACCAGCTC CTGTAAAAAA AGAAACTGAA
    GTCATCAAAG AAAAAGAAAA AACAGCTCCT GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG
    AAAACGAAAC CAACCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAT AGTTAAACAA
    GCTCCTGTGA AGAAAGAGGC TGAACCTGTA AAGGAGATAA CTAAACCAGC CCCTGAGAAG
    AAAGAGGCTG AAATAATCAA GGAGAAAGTT AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG
    GAGAAAGCAA AACCAGCTCC TGTAAAGAAA GAAGCTGAAG TCATCAAACA AAAAGCCAAA
    CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC ATCAAGGAGA AAGTTAAACC GGCTCCTGTA
    AAGATAGAGG CTAAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAGGT CAAATCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG
    GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG GCTGAAGTCA TCAAGGAGAA AGTTAAACCT
    GCTCCTGTGA GGAAAGAAGC TGAAGAAATA AAGGAAAAAG CTGAAGCAGT TTATGAGAAA
    AAAGAGGCTG AAGTAGCCAA GGAGATAGTT AAACCAACTC CTTTAAAGAA GGAGCCCGAA
    GTTGTGAAGG AAAAGGCTCC TGAATCAAAA CCCCAAAAAG AGGCTGAAAT TATCAAAGAG
    AAAGTTAAAC CTGCACCTGT AAAGAAAGAG CCTGAAGTTG CAAAAGTACC AAAACCCAAA
    AAAGAGGCTG AACTCGCCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAAGAAAAA
    CCTAAGGCAG CACCAAAGAA AGAGGCTGAA GCTATTAAGG AGAAACGTAC ACCAGCTCCT
    GAAAAGAAAG TGGTAGAAGT CATCAAGGAG AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG
    GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA
    AAGGCTCCTG AACCAAAACC CCAAAAAGAG GTTGAAGTCA TCAAAGAGAA AGCTAAACCA
    GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG GAAAAGGTTC AACCAGCTCC TATAAAGAAA
    GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT GAACCAACAC CAGAACCTGA GAGAGTTGAA
    GCTCCAAAAG AAAAGGCAAA AGTAATCCCT AGGGAAAAAG TAGCTGAGAT TACTAAAGAA
    AAACCAAAGC CAGCACCAAA GAAAGAGGCT GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT
    GTGCACAAAG AGGTTGAAGT GTTCAAGGAG AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG
    CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA CCAAAACCTA AAAAGACTGA AGTCATCAAA
    GCGAAAGTAA AACCAGCCCC TGAGAAAAAA GAGGCTGAAG TGAAGGAGAA AATTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATA AAGGAGAGAA TTAAACCTGC TCCTGTAAAG
    AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCCAAAAA AGAACCTGAA
    GTTATCAAAG AGAAAGTGAA ACCAGCGCCA GAGAAGAAAG CACCCGAAAT AACCAAAGAA
    AAACCAAAGC CAGCAGCAAA GAAAGTAGCT GAAGTCATCA AAGACAGAAC TAAACCAGTC
    CCTGAGAAGA AAGAGGCTGA AGTCATCAAG GAGAAACCAA AAGCAGTTCC CGAGAAGAAA
    GAGGCTAAAG TCATTGAAAA GAAAGTTATA CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT
    GTTAAGGAAA AGGCTCCTGA ACTGACAACA AAAAAAGCAC CTGAGATAAC CAAAGAAAAA
    CCGAAGCCAG TACCAAAGAA AGAGGCTGAA GTCATTAAAG AGAAAGCTAA ACTAGCTCCT
    GAAAAGAAAG AGGTTGAAGT CATCAAGGAG AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG
    CCTGAGGTTC ATAAGGAAAA AGCACCTGAA CCAAAATCAG AACCTACAAA AAAAGTTGAG
    GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT AAAGAAAAGG AACCTGAAAT AACCAAAGAA
    AAGCCAAAAC CAGCACCAAA GAAAGAAGCT GTAGTCATCA AAGAAAAAGC TGTACCAGCC
    ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT
    GAAATTAAGG AGAAAGTTAA ACCAGCTTCT ATAATGAAAG AGCCTGAAGT TGTGAAACAA
    AAGACTCCTG AACCGAAACC CAAAAAAGAG CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA
    CCTAAACCCA AAAAAGAGCC TGAAGTTGCT AAAGAGAAAG CCAAACCAGC ACCTCAGAAG
    AAAGAAGTCA TCAAAGAAAA AGTCAAACTT GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC
    GAAGAAAAGG CACTTAAACC AGAACCCAAA AAAGATGAAG TTGGTCTTAT TGGTCTGGAC
    CTGGAGCTCC TAAACTCCAT CAACCAAAAA CTGTCTCTCC TGGACTTGCT AAGGAAGGAC
    ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC ACTCAGAATC AAATCACACT TTTACGGATG
    GACAACAGAA CGATCAAAGA GCCAGAGACT ATTGGTGTGA AAGTAAAACC AGCAGTCAAG
    GAAAAAGCAA AAAGAGACCA CGCTCTGATC AATGCTACTA GGACTGCACA TATTAAAACA
    GAGCGTGAAT CTCTAAAAAA CATCACGAAA CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT
    CCAAAGGTTA AGATTGCGCT GGAGCCTAAA AAGAAAGGAA TCATCCCTGA GAAGGTCAAA
    GAAGTAAAGG AAACTTTACA GAAAGAACCT GAGTTGGTCA AAGATAAAGT AGTTGAGCCA
    ATAGAGAAGG AAATTGTTGT TGAGAAGGAA GTCAAAGGAG AAGAGCCTGA AATCACCAAA
    GATATTCCAG AGGTGGAGGA AGAGGACATT CCCTACTTCC AGTGTTTCTT TGTGGATGAG
    GATGACACTC AGTATCCGTT CTTCCCTTTC TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149702
    Clone ID Related Accession (Same CDS sequence) XM_021467366.1
    Accession Version XM_021467366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAAGAAAAAC CTAAGGCAGC ACCAAAGAAA
    GAGGCTGAAG CTATTAAGGA GAAACGTACA CCAGCTCCTG AAAAGAAAGT GGTAGAAGTC
    ATCAAGGAGA AAGTCAAACC AGCCCCTGAG AAAAAAGCGG TTCTCAAGGA GAAAGTTAGA
    CCTGCTCCTG AAAAGAAAGA GCCTGAAGTT GCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    CAAAAAGAGG TTGAAGTCAT CAAAGAGAAA GCTAAACCAG CCCTTAAGAA GAAAGAGCCT
    AAAGTCAAGG AAAAGGTTCA ACCAGCTCCT ATAAAGAAAG AGCCAGAGCT TCCCAAAGAA
    AAGGTGCCTG AACCAACACC AGAACCTGAG AGAGTTGAAG CTCCAAAAGA AAAGGCAAAA
    GTAATCCCTA GGGAAAAAGT AGCTGAGATT ACTAAAGAAA AACCAAAGCC AGCACCAAAG
    AAAGAGGCTG AAGTAAAGGA GAAAGCTACA CCTGCTCCTG TGCACAAAGA GGTTGAAGTG
    TTCAAGGAGA AAGTTAAACA AGCTCCTGTA AAGAAAGAGC CAGAAGTTCC TAAAGAAAAG
    GCTCCTGAAC CAAAACCTAA AAAGACTGAA GTCATCAAAG CGAAAGTAAA ACCAGCCCCT
    GAGAAAAAAG AGGCTGAAGT GAAGGAGAAA ATTAAACCAG TCCCTGAGAA GAAAGAGGCT
    GAAGTCATAA AGGAGAGAAT TAAACCTGCT CCTGTAAAGA AAGAGCCTGA AGTTCCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCAAAAAA GAACCTGAAG TTATCAAAGA GAAAGTGAAA
    CCAGCGCCAG AGAAGAAAGC ACCCGAAATA ACCAAAGAAA AACCAAAGCC AGCAGCAAAG
    AAAGTAGCTG AAGTCATCAA AGACAGAACT AAACCAGTCC CTGAGAAGAA AGAGGCTGAA
    GTCATCAAGG AGAAACCAAA AGCAGTTCCC GAGAAGAAAG AGGCTAAAGT CATTGAAAAG
    AAAGTTATAC CAGCTCCCGT AAAGAAAGAG CCTGAAGTTG TTAAGGAAAA GGCTCCTGAA
    CTGACAACAA AAAAAGCACC TGAGATAACC AAAGAAAAAC CGAAGCCAGT ACCAAAGAAA
    GAGGCTGAAG TCATTAAAGA GAAAGCTAAA CTAGCTCCTG AAAAGAAAGA GGTTGAAGTC
    ATCAAGGAGA AAGTTAAACC AGCTCCGTTA AAGAAAGAGC CTGAGGTTCA TAAGGAAAAA
    GCACCTGAAC CAAAATCAGA ACCTACAAAA AAAGTTGAGG TTCCAAAGGA AAAGGCAAAA
    CCAGTTGCTA AAGAAAAGGA ACCTGAAATA ACCAAAGAAA AGCCAAAACC AGCACCAAAG
    AAAGAAGCTG TAGTCATCAA AGAAAAAGCT GTACCAGCCA TCCTGAAGGA AGTGGCTGAA
    GTAAAGAAAG TTAAAGCAGC TCCTGAGAAG AAAGTGGCTG AAATTAAGGA GAAAGTTAAA
    CCAGCTTCTA TAATGAAAGA GCCTGAAGTT GTGAAACAAA AGACTCCTGA ACCGAAACCC
    AAAAAAGAGC CTGAAGTTGT GAAAGAAAAG GCTCCTGAAC CTAAACCCAA AAAAGAGCCT
    GAAGTTGCTA AAGAGAAAGC CAAACCAGCA CCTCAGAAGA AAGAAGTCAT CAAAGAAAAA
    GTCAAACTTG CTCCTGTAAA GAAAGTGCCC GAAGTTGTCG AAGAAAAGGC ACTTAAACCA
    GAACCCAAAA AAGATGAAGT TGGTCTTATT GGTCTGGACC TGGAGCTCCT AAACTCCATC
    AACCAAAAAC TGTCTCTCCT GGACTTGCTA AGGAAGGACA TTGGAGAAAT GAAAAGCGAT
    CTGGAGTCCA CTCAGAATCA AATCACACTT TTACGGATGG ACAACAGAAC GATCAAAGAG
    CCAGAGACTA TTGGTGTGAA AGTAAAACCA GCAGTCAAGG AAAAAGCAAA AAGAGACCAC
    GCTCTGATCA ATGCTACTAG GACTGCACAT ATTAAAACAG AGCGTGAATC TCTAAAAAAC
    ATCACGAAAC CAGTGCCTGC AAAGAAAGCC AAAGAGGCTC CAAAGGTTAA GATTGCGCTG
    GAGCCTAAAA AGAAAGGAAT CATCCCTGAG AAGGTCAAAG AAGTAAAGGA AACTTTACAG
    AAAGAACCTG AGTTGGTCAA AGATAAAGTA GTTGAGCCAA TAGAGAAGGA AATTGTTGTT
    GAGAAGGAAG TCAAAGGAGA AGAGCCTGAA ATCACCAAAG ATATTCCAGA GGTGGAGGAA
    GAGGACATTC CCTACTTCCA GTGTTTCTTT GTGGATGAGG ATGACACTCA GTATCCGTTC
    TTCCCTTTCT CTCCACCGCT TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323041.1
    CDS338..8284
    Translation

    Target ORF information:

    RefSeq Version XM_021467366.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAAGAAAAAC CTAAGGCAGC ACCAAAGAAA
    GAGGCTGAAG CTATTAAGGA GAAACGTACA CCAGCTCCTG AAAAGAAAGT GGTAGAAGTC
    ATCAAGGAGA AAGTCAAACC AGCCCCTGAG AAAAAAGCGG TTCTCAAGGA GAAAGTTAGA
    CCTGCTCCTG AAAAGAAAGA GCCTGAAGTT GCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    CAAAAAGAGG TTGAAGTCAT CAAAGAGAAA GCTAAACCAG CCCTTAAGAA GAAAGAGCCT
    AAAGTCAAGG AAAAGGTTCA ACCAGCTCCT ATAAAGAAAG AGCCAGAGCT TCCCAAAGAA
    AAGGTGCCTG AACCAACACC AGAACCTGAG AGAGTTGAAG CTCCAAAAGA AAAGGCAAAA
    GTAATCCCTA GGGAAAAAGT AGCTGAGATT ACTAAAGAAA AACCAAAGCC AGCACCAAAG
    AAAGAGGCTG AAGTAAAGGA GAAAGCTACA CCTGCTCCTG TGCACAAAGA GGTTGAAGTG
    TTCAAGGAGA AAGTTAAACA AGCTCCTGTA AAGAAAGAGC CAGAAGTTCC TAAAGAAAAG
    GCTCCTGAAC CAAAACCTAA AAAGACTGAA GTCATCAAAG CGAAAGTAAA ACCAGCCCCT
    GAGAAAAAAG AGGCTGAAGT GAAGGAGAAA ATTAAACCAG TCCCTGAGAA GAAAGAGGCT
    GAAGTCATAA AGGAGAGAAT TAAACCTGCT CCTGTAAAGA AAGAGCCTGA AGTTCCTAAA
    GAAAAGGCTC CTGAACCAAA ACCCAAAAAA GAACCTGAAG TTATCAAAGA GAAAGTGAAA
    CCAGCGCCAG AGAAGAAAGC ACCCGAAATA ACCAAAGAAA AACCAAAGCC AGCAGCAAAG
    AAAGTAGCTG AAGTCATCAA AGACAGAACT AAACCAGTCC CTGAGAAGAA AGAGGCTGAA
    GTCATCAAGG AGAAACCAAA AGCAGTTCCC GAGAAGAAAG AGGCTAAAGT CATTGAAAAG
    AAAGTTATAC CAGCTCCCGT AAAGAAAGAG CCTGAAGTTG TTAAGGAAAA GGCTCCTGAA
    CTGACAACAA AAAAAGCACC TGAGATAACC AAAGAAAAAC CGAAGCCAGT ACCAAAGAAA
    GAGGCTGAAG TCATTAAAGA GAAAGCTAAA CTAGCTCCTG AAAAGAAAGA GGTTGAAGTC
    ATCAAGGAGA AAGTTAAACC AGCTCCGTTA AAGAAAGAGC CTGAGGTTCA TAAGGAAAAA
    GCACCTGAAC CAAAATCAGA ACCTACAAAA AAAGTTGAGG TTCCAAAGGA AAAGGCAAAA
    CCAGTTGCTA AAGAAAAGGA ACCTGAAATA ACCAAAGAAA AGCCAAAACC AGCACCAAAG
    AAAGAAGCTG TAGTCATCAA AGAAAAAGCT GTACCAGCCA TCCTGAAGGA AGTGGCTGAA
    GTAAAGAAAG TTAAAGCAGC TCCTGAGAAG AAAGTGGCTG AAATTAAGGA GAAAGTTAAA
    CCAGCTTCTA TAATGAAAGA GCCTGAAGTT GTGAAACAAA AGACTCCTGA ACCGAAACCC
    AAAAAAGAGC CTGAAGTTGT GAAAGAAAAG GCTCCTGAAC CTAAACCCAA AAAAGAGCCT
    GAAGTTGCTA AAGAGAAAGC CAAACCAGCA CCTCAGAAGA AAGAAGTCAT CAAAGAAAAA
    GTCAAACTTG CTCCTGTAAA GAAAGTGCCC GAAGTTGTCG AAGAAAAGGC ACTTAAACCA
    GAACCCAAAA AAGATGAAGT TGGTCTTATT GGTCTGGACC TGGAGCTCCT AAACTCCATC
    AACCAAAAAC TGTCTCTCCT GGACTTGCTA AGGAAGGACA TTGGAGAAAT GAAAAGCGAT
    CTGGAGTCCA CTCAGAATCA AATCACACTT TTACGGATGG ACAACAGAAC GATCAAAGAG
    CCAGAGACTA TTGGTGTGAA AGTAAAACCA GCAGTCAAGG AAAAAGCAAA AAGAGACCAC
    GCTCTGATCA ATGCTACTAG GACTGCACAT ATTAAAACAG AGCGTGAATC TCTAAAAAAC
    ATCACGAAAC CAGTGCCTGC AAAGAAAGCC AAAGAGGCTC CAAAGGTTAA GATTGCGCTG
    GAGCCTAAAA AGAAAGGAAT CATCCCTGAG AAGGTCAAAG AAGTAAAGGA AACTTTACAG
    AAAGAACCTG AGTTGGTCAA AGATAAAGTA GTTGAGCCAA TAGAGAAGGA AATTGTTGTT
    GAGAAGGAAG TCAAAGGAGA AGAGCCTGAA ATCACCAAAG ATATTCCAGA GGTGGAGGAA
    GAGGACATTC CCTACTTCCA GTGTTTCTTT GTGGATGAGG ATGACACTCA GTATCCGTTC
    TTCCCTTTCT CTCCACCGCT TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149703
    Clone ID Related Accession (Same CDS sequence) XM_021467367.1
    Accession Version XM_021467367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAAGA GAAAGCTAAA CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC
    AAGGAAAAGG TTCAACCAGC TCCTATAAAG AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG
    CCTGAACCAA CACCAGAACC TGAGAGAGTT GAAGCTCCAA AAGAAAAGGC AAAAGTAATC
    CCTAGGGAAA AAGTAGCTGA GATTACTAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGTAA AGGAGAAAGC TACACCTGCT CCTGTGCACA AAGAGGTTGA AGTGTTCAAG
    GAGAAAGTTA AACAAGCTCC TGTAAAGAAA GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT
    GAACCAAAAC CTAAAAAGAC TGAAGTCATC AAAGCGAAAG TAAAACCAGC CCCTGAGAAA
    AAAGAGGCTG AAGTGAAGGA GAAAATTAAA CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC
    ATAAAGGAGA GAATTAAACC TGCTCCTGTA AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG
    GCTCCTGAAC CAAAACCCAA AAAAGAACCT GAAGTTATCA AAGAGAAAGT GAAACCAGCG
    CCAGAGAAGA AAGCACCCGA AATAACCAAA GAAAAACCAA AGCCAGCAGC AAAGAAAGTA
    GCTGAAGTCA TCAAAGACAG AACTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG AAAGAGGCTA AAGTCATTGA AAAGAAAGTT
    ATACCAGCTC CCGTAAAGAA AGAGCCTGAA GTTGTTAAGG AAAAGGCTCC TGAACTGACA
    ACAAAAAAAG CACCTGAGAT AACCAAAGAA AAACCGAAGC CAGTACCAAA GAAAGAGGCT
    GAAGTCATTA AAGAGAAAGC TAAACTAGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAG
    GAGAAAGTTA AACCAGCTCC GTTAAAGAAA GAGCCTGAGG TTCATAAGGA AAAAGCACCT
    GAACCAAAAT CAGAACCTAC AAAAAAAGTT GAGGTTCCAA AGGAAAAGGC AAAACCAGTT
    GCTAAAGAAA AGGAACCTGA AATAACCAAA GAAAAGCCAA AACCAGCACC AAAGAAAGAA
    GCTGTAGTCA TCAAAGAAAA AGCTGTACCA GCCATCCTGA AGGAAGTGGC TGAAGTAAAG
    AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG GCTGAAATTA AGGAGAAAGT TAAACCAGCT
    TCTATAATGA AAGAGCCTGA AGTTGTGAAA CAAAAGACTC CTGAACCGAA ACCCAAAAAA
    GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT GAACCTAAAC CCAAAAAAGA GCCTGAAGTT
    GCTAAAGAGA AAGCCAAACC AGCACCTCAG AAGAAAGAAG TCATCAAAGA AAAAGTCAAA
    CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT GTCGAAGAAA AGGCACTTAA ACCAGAACCC
    AAAAAAGATG AAGTTGGTCT TATTGGTCTG GACCTGGAGC TCCTAAACTC CATCAACCAA
    AAACTGTCTC TCCTGGACTT GCTAAGGAAG GACATTGGAG AAATGAAAAG CGATCTGGAG
    TCCACTCAGA ATCAAATCAC ACTTTTACGG ATGGACAACA GAACGATCAA AGAGCCAGAG
    ACTATTGGTG TGAAAGTAAA ACCAGCAGTC AAGGAAAAAG CAAAAAGAGA CCACGCTCTG
    ATCAATGCTA CTAGGACTGC ACATATTAAA ACAGAGCGTG AATCTCTAAA AAACATCACG
    AAACCAGTGC CTGCAAAGAA AGCCAAAGAG GCTCCAAAGG TTAAGATTGC GCTGGAGCCT
    AAAAAGAAAG GAATCATCCC TGAGAAGGTC AAAGAAGTAA AGGAAACTTT ACAGAAAGAA
    CCTGAGTTGG TCAAAGATAA AGTAGTTGAG CCAATAGAGA AGGAAATTGT TGTTGAGAAG
    GAAGTCAAAG GAGAAGAGCC TGAAATCACC AAAGATATTC CAGAGGTGGA GGAAGAGGAC
    ATTCCCTACT TCCAGTGTTT CTTTGTGGAT GAGGATGACA CTCAGTATCC GTTCTTCCCT
    TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323042.1
    CDS338..8278
    Translation

    Target ORF information:

    RefSeq Version XM_021467367.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAAGA GAAAGCTAAA CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC
    AAGGAAAAGG TTCAACCAGC TCCTATAAAG AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG
    CCTGAACCAA CACCAGAACC TGAGAGAGTT GAAGCTCCAA AAGAAAAGGC AAAAGTAATC
    CCTAGGGAAA AAGTAGCTGA GATTACTAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGTAA AGGAGAAAGC TACACCTGCT CCTGTGCACA AAGAGGTTGA AGTGTTCAAG
    GAGAAAGTTA AACAAGCTCC TGTAAAGAAA GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT
    GAACCAAAAC CTAAAAAGAC TGAAGTCATC AAAGCGAAAG TAAAACCAGC CCCTGAGAAA
    AAAGAGGCTG AAGTGAAGGA GAAAATTAAA CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC
    ATAAAGGAGA GAATTAAACC TGCTCCTGTA AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG
    GCTCCTGAAC CAAAACCCAA AAAAGAACCT GAAGTTATCA AAGAGAAAGT GAAACCAGCG
    CCAGAGAAGA AAGCACCCGA AATAACCAAA GAAAAACCAA AGCCAGCAGC AAAGAAAGTA
    GCTGAAGTCA TCAAAGACAG AACTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG AAAGAGGCTA AAGTCATTGA AAAGAAAGTT
    ATACCAGCTC CCGTAAAGAA AGAGCCTGAA GTTGTTAAGG AAAAGGCTCC TGAACTGACA
    ACAAAAAAAG CACCTGAGAT AACCAAAGAA AAACCGAAGC CAGTACCAAA GAAAGAGGCT
    GAAGTCATTA AAGAGAAAGC TAAACTAGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAG
    GAGAAAGTTA AACCAGCTCC GTTAAAGAAA GAGCCTGAGG TTCATAAGGA AAAAGCACCT
    GAACCAAAAT CAGAACCTAC AAAAAAAGTT GAGGTTCCAA AGGAAAAGGC AAAACCAGTT
    GCTAAAGAAA AGGAACCTGA AATAACCAAA GAAAAGCCAA AACCAGCACC AAAGAAAGAA
    GCTGTAGTCA TCAAAGAAAA AGCTGTACCA GCCATCCTGA AGGAAGTGGC TGAAGTAAAG
    AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG GCTGAAATTA AGGAGAAAGT TAAACCAGCT
    TCTATAATGA AAGAGCCTGA AGTTGTGAAA CAAAAGACTC CTGAACCGAA ACCCAAAAAA
    GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT GAACCTAAAC CCAAAAAAGA GCCTGAAGTT
    GCTAAAGAGA AAGCCAAACC AGCACCTCAG AAGAAAGAAG TCATCAAAGA AAAAGTCAAA
    CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT GTCGAAGAAA AGGCACTTAA ACCAGAACCC
    AAAAAAGATG AAGTTGGTCT TATTGGTCTG GACCTGGAGC TCCTAAACTC CATCAACCAA
    AAACTGTCTC TCCTGGACTT GCTAAGGAAG GACATTGGAG AAATGAAAAG CGATCTGGAG
    TCCACTCAGA ATCAAATCAC ACTTTTACGG ATGGACAACA GAACGATCAA AGAGCCAGAG
    ACTATTGGTG TGAAAGTAAA ACCAGCAGTC AAGGAAAAAG CAAAAAGAGA CCACGCTCTG
    ATCAATGCTA CTAGGACTGC ACATATTAAA ACAGAGCGTG AATCTCTAAA AAACATCACG
    AAACCAGTGC CTGCAAAGAA AGCCAAAGAG GCTCCAAAGG TTAAGATTGC GCTGGAGCCT
    AAAAAGAAAG GAATCATCCC TGAGAAGGTC AAAGAAGTAA AGGAAACTTT ACAGAAAGAA
    CCTGAGTTGG TCAAAGATAA AGTAGTTGAG CCAATAGAGA AGGAAATTGT TGTTGAGAAG
    GAAGTCAAAG GAGAAGAGCC TGAAATCACC AAAGATATTC CAGAGGTGGA GGAAGAGGAC
    ATTCCCTACT TCCAGTGTTT CTTTGTGGAT GAGGATGACA CTCAGTATCC GTTCTTCCCT
    TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149704
    Clone ID Related Accession (Same CDS sequence) XM_021467368.1
    Accession Version XM_021467368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGATG AAGTTGGTCT TATTGGTCTG GACCTGGAGC TCCTAAACTC CATCAACCAA
    AAACTGTCTC TCCTGGACTT GCTAAGGAAG GACATTGGAG AAATGAAAAG CGATCTGGAG
    TCCACTCAGA ATCAAATCAC ACTTTTACGG ATGGACAACA GAACGATCAA AGAGCCAGAG
    ACTATTGGTG TGAAAGTAAA ACCAGCAGTC AAGGAAAAAG CAAAAAGAGA CCACGCTCTG
    ATCAATGCTA CTAGGACTGC ACATATTAAA ACAGAGCGTG AATCTCTAAA AAACATCACG
    AAACCAGTGC CTGCAAAGAA AGCCAAAGAG GCTCCAAAGG TTAAGATTGC GCTGGAGCCT
    AAAAAGAAAG GAATCATCCC TGAGAAGGTC AAAGAAGTAA AGGAAACTTT ACAGAAAGAA
    CCTGAGTTGG TCAAAGATAA AGTAGTTGAG CCAATAGAGA AGGAAATTGT TGTTGAGAAG
    GAAGTCAAAG GAGAAGAGCC TGAAATCACC AAAGATATTC CAGAGGTGGA GGAAGAGGAC
    ATTCCCTACT TCCAGTGTTT CTTTGTGGAT GAGGATGACA CTCAGTATCC GTTCTTCCCT
    TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323043.1
    CDS338..8278
    Translation

    Target ORF information:

    RefSeq Version XM_021467368.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGATG AAGTTGGTCT TATTGGTCTG GACCTGGAGC TCCTAAACTC CATCAACCAA
    AAACTGTCTC TCCTGGACTT GCTAAGGAAG GACATTGGAG AAATGAAAAG CGATCTGGAG
    TCCACTCAGA ATCAAATCAC ACTTTTACGG ATGGACAACA GAACGATCAA AGAGCCAGAG
    ACTATTGGTG TGAAAGTAAA ACCAGCAGTC AAGGAAAAAG CAAAAAGAGA CCACGCTCTG
    ATCAATGCTA CTAGGACTGC ACATATTAAA ACAGAGCGTG AATCTCTAAA AAACATCACG
    AAACCAGTGC CTGCAAAGAA AGCCAAAGAG GCTCCAAAGG TTAAGATTGC GCTGGAGCCT
    AAAAAGAAAG GAATCATCCC TGAGAAGGTC AAAGAAGTAA AGGAAACTTT ACAGAAAGAA
    CCTGAGTTGG TCAAAGATAA AGTAGTTGAG CCAATAGAGA AGGAAATTGT TGTTGAGAAG
    GAAGTCAAAG GAGAAGAGCC TGAAATCACC AAAGATATTC CAGAGGTGGA GGAAGAGGAC
    ATTCCCTACT TCCAGTGTTT CTTTGTGGAT GAGGATGACA CTCAGTATCC GTTCTTCCCT
    TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149705
    Clone ID Related Accession (Same CDS sequence) XM_021467369.1
    Accession Version XM_021467369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG AAAACGAAAC CAACCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAT AGTTAAACAA GCTCCTGTGA AGAAAGAGGC TGAACCTGTA
    AAGGAGATAA CTAAACCAGC CCCTGAGAAG AAAGAGGCTG AAATAATCAA GGAGAAAGTT
    AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG GAGAAAGCAA AACCAGCTCC TGTAAAGAAA
    GAAGCTGAAG TCATCAAACA AAAAGCCAAA CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC
    ATCAAGGAGA AAGTTAAACC GGCTCCTGTA AAGATAGAGG CTAAACCTGT AAAGGAGATA
    ACTAAACCAG CCCCTGAGAA GAAAGAGGCT GAAATAATCA AGGAGAAGGT CAAATCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCT GCTCCTGTGA GGAAAGAAGC TGAAGAAATA
    AAGGAAAAAG CTGAAGCAGT TTATGAGAAA AAAGAGGCTG AAGTAGCCAA GGAGATAGTT
    AAACCAACTC CTTTAAAGAA GGAGCCCGAA GTTGTGAAGG AAAAGGCTCC TGAATCAAAA
    CCCCAAAAAG AGGCTGAAAT TATCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAG
    CCTGAAGTTG CAAAAGTACC AAAACCCAAA AAAGAGGCTG AACTCGCCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAAGAAAAA CCTAAGGCAG CACCAAAGAA AGAGGCTGAA
    GCTATTAAGG AGAAACGTAC ACCAGCTCCT GAAAAGAAAG TGGTAGAAGT CATCAAGGAG
    AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT
    GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA AAGGCTCCTG AACCAAAACC CCAAAAAGAG
    GTTGAAGTCA TCAAAGAGAA AGCTAAACCA GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG
    GAAAAGGTTC AACCAGCTCC TATAAAGAAA GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT
    GAACCAACAC CAGAACCTGA GAGAGTTGAA GCTCCAAAAG AAAAGGCAAA AGTAATCCCT
    AGGGAAAAAG TAGCTGAGAT TACTAAAGAA AAACCAAAGC CAGCACCAAA GAAAGAGGCT
    GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT GTGCACAAAG AGGTTGAAGT GTTCAAGGAG
    AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA
    CCAAAACCTA AAAAGACTGA AGTCATCAAA GCGAAAGTAA AACCAGCCCC TGAGAAAAAA
    GAGGCTGAAG TGAAGGAGAA AATTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATA
    AAGGAGAGAA TTAAACCTGC TCCTGTAAAG AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT
    CCTGAACCAA AACCCAAAAA AGAACCTGAA GTTATCAAAG AGAAAGTGAA ACCAGCGCCA
    GAGAAGAAAG CACCCGAAAT AACCAAAGAA AAACCAAAGC CAGCAGCAAA GAAAGTAGCT
    GAAGTCATCA AAGACAGAAC TAAACCAGTC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAACCAA AAGCAGTTCC CGAGAAGAAA GAGGCTAAAG TCATTGAAAA GAAAGTTATA
    CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT GTTAAGGAAA AGGCTCCTGA ACTGACAACA
    AAAAAAGCAC CTGAGATAAC CAAAGAAAAA CCGAAGCCAG TACCAAAGAA AGAGGCTGAA
    GTCATTAAAG AGAAAGCTAA ACTAGCTCCT GAAAAGAAAG AGGTTGAAGT CATCAAGGAG
    AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG CCTGAGGTTC ATAAGGAAAA AGCACCTGAA
    CCAAAATCAG AACCTACAAA AAAAGTTGAG GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT
    AAAGAAAAGG AACCTGAAAT AACCAAAGAA AAGCCAAAAC CAGCACCAAA GAAAGAAGCT
    GTAGTCATCA AAGAAAAAGC TGTACCAGCC ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA
    GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT GAAATTAAGG AGAAAGTTAA ACCAGCTTCT
    ATAATGAAAG AGCCTGAAGT TGTGAAACAA AAGACTCCTG AACCGAAACC CAAAAAAGAG
    CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA CCTAAACCCA AAAAAGAGCC TGAAGTTGCT
    AAAGAGAAAG CCAAACCAGC ACCTCAGAAG AAAGAAGTCA TCAAAGAAAA AGTCAAACTT
    GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC GAAGAAAAGG CACTTAAACC AGAACCCAAA
    AAAGATGAAG TTGGTCTTAT TGGTCTGGAC CTGGAGCTCC TAAACTCCAT CAACCAAAAA
    CTGTCTCTCC TGGACTTGCT AAGGAAGGAC ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC
    ACTCAGAATC AAATCACACT TTTACGGATG GACAACAGAA CGATCAAAGA GCCAGAGACT
    ATTGGTGTGA AAGTAAAACC AGCAGTCAAG GAAAAAGCAA AAAGAGACCA CGCTCTGATC
    AATGCTACTA GGACTGCACA TATTAAAACA GAGCGTGAAT CTCTAAAAAA CATCACGAAA
    CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT CCAAAGGTTA AGATTGCGCT GGAGCCTAAA
    AAGAAAGGAA TCATCCCTGA GAAGGTCAAA GAAGTAAAGG AAACTTTACA GAAAGAACCT
    GAGTTGGTCA AAGATAAAGT AGTTGAGCCA ATAGAGAAGG AAATTGTTGT TGAGAAGGAA
    GTCAAAGGAG AAGAGCCTGA AATCACCAAA GATATTCCAG AGGTGGAGGA AGAGGACATT
    CCCTACTTCC AGTGTTTCTT TGTGGATGAG GATGACACTC AGTATCCGTT CTTCCCTTTC
    TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323044.1
    CDS338..8275
    Translation

    Target ORF information:

    RefSeq Version XM_021467369.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG AAAACGAAAC CAACCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAT AGTTAAACAA GCTCCTGTGA AGAAAGAGGC TGAACCTGTA
    AAGGAGATAA CTAAACCAGC CCCTGAGAAG AAAGAGGCTG AAATAATCAA GGAGAAAGTT
    AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG GAGAAAGCAA AACCAGCTCC TGTAAAGAAA
    GAAGCTGAAG TCATCAAACA AAAAGCCAAA CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC
    ATCAAGGAGA AAGTTAAACC GGCTCCTGTA AAGATAGAGG CTAAACCTGT AAAGGAGATA
    ACTAAACCAG CCCCTGAGAA GAAAGAGGCT GAAATAATCA AGGAGAAGGT CAAATCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCT GCTCCTGTGA GGAAAGAAGC TGAAGAAATA
    AAGGAAAAAG CTGAAGCAGT TTATGAGAAA AAAGAGGCTG AAGTAGCCAA GGAGATAGTT
    AAACCAACTC CTTTAAAGAA GGAGCCCGAA GTTGTGAAGG AAAAGGCTCC TGAATCAAAA
    CCCCAAAAAG AGGCTGAAAT TATCAAAGAG AAAGTTAAAC CTGCACCTGT AAAGAAAGAG
    CCTGAAGTTG CAAAAGTACC AAAACCCAAA AAAGAGGCTG AACTCGCCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAAGAAAAA CCTAAGGCAG CACCAAAGAA AGAGGCTGAA
    GCTATTAAGG AGAAACGTAC ACCAGCTCCT GAAAAGAAAG TGGTAGAAGT CATCAAGGAG
    AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT
    GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA AAGGCTCCTG AACCAAAACC CCAAAAAGAG
    GTTGAAGTCA TCAAAGAGAA AGCTAAACCA GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG
    GAAAAGGTTC AACCAGCTCC TATAAAGAAA GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT
    GAACCAACAC CAGAACCTGA GAGAGTTGAA GCTCCAAAAG AAAAGGCAAA AGTAATCCCT
    AGGGAAAAAG TAGCTGAGAT TACTAAAGAA AAACCAAAGC CAGCACCAAA GAAAGAGGCT
    GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT GTGCACAAAG AGGTTGAAGT GTTCAAGGAG
    AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA
    CCAAAACCTA AAAAGACTGA AGTCATCAAA GCGAAAGTAA AACCAGCCCC TGAGAAAAAA
    GAGGCTGAAG TGAAGGAGAA AATTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATA
    AAGGAGAGAA TTAAACCTGC TCCTGTAAAG AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT
    CCTGAACCAA AACCCAAAAA AGAACCTGAA GTTATCAAAG AGAAAGTGAA ACCAGCGCCA
    GAGAAGAAAG CACCCGAAAT AACCAAAGAA AAACCAAAGC CAGCAGCAAA GAAAGTAGCT
    GAAGTCATCA AAGACAGAAC TAAACCAGTC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAACCAA AAGCAGTTCC CGAGAAGAAA GAGGCTAAAG TCATTGAAAA GAAAGTTATA
    CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT GTTAAGGAAA AGGCTCCTGA ACTGACAACA
    AAAAAAGCAC CTGAGATAAC CAAAGAAAAA CCGAAGCCAG TACCAAAGAA AGAGGCTGAA
    GTCATTAAAG AGAAAGCTAA ACTAGCTCCT GAAAAGAAAG AGGTTGAAGT CATCAAGGAG
    AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG CCTGAGGTTC ATAAGGAAAA AGCACCTGAA
    CCAAAATCAG AACCTACAAA AAAAGTTGAG GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT
    AAAGAAAAGG AACCTGAAAT AACCAAAGAA AAGCCAAAAC CAGCACCAAA GAAAGAAGCT
    GTAGTCATCA AAGAAAAAGC TGTACCAGCC ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA
    GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT GAAATTAAGG AGAAAGTTAA ACCAGCTTCT
    ATAATGAAAG AGCCTGAAGT TGTGAAACAA AAGACTCCTG AACCGAAACC CAAAAAAGAG
    CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA CCTAAACCCA AAAAAGAGCC TGAAGTTGCT
    AAAGAGAAAG CCAAACCAGC ACCTCAGAAG AAAGAAGTCA TCAAAGAAAA AGTCAAACTT
    GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC GAAGAAAAGG CACTTAAACC AGAACCCAAA
    AAAGATGAAG TTGGTCTTAT TGGTCTGGAC CTGGAGCTCC TAAACTCCAT CAACCAAAAA
    CTGTCTCTCC TGGACTTGCT AAGGAAGGAC ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC
    ACTCAGAATC AAATCACACT TTTACGGATG GACAACAGAA CGATCAAAGA GCCAGAGACT
    ATTGGTGTGA AAGTAAAACC AGCAGTCAAG GAAAAAGCAA AAAGAGACCA CGCTCTGATC
    AATGCTACTA GGACTGCACA TATTAAAACA GAGCGTGAAT CTCTAAAAAA CATCACGAAA
    CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT CCAAAGGTTA AGATTGCGCT GGAGCCTAAA
    AAGAAAGGAA TCATCCCTGA GAAGGTCAAA GAAGTAAAGG AAACTTTACA GAAAGAACCT
    GAGTTGGTCA AAGATAAAGT AGTTGAGCCA ATAGAGAAGG AAATTGTTGT TGAGAAGGAA
    GTCAAAGGAG AAGAGCCTGA AATCACCAAA GATATTCCAG AGGTGGAGGA AGAGGACATT
    CCCTACTTCC AGTGTTTCTT TGTGGATGAG GATGACACTC AGTATCCGTT CTTCCCTTTC
    TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149706
    Clone ID Related Accession (Same CDS sequence) XM_021467370.1
    Accession Version XM_021467370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X39
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCTGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCC AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA
    GTGAAACCAG CGCCAGAGAA GAAAGCACCC GAAATAACCA AAGAAAAACC AAAGCCAGCA
    GCAAAGAAAG TAGCTGAAGT CATCAAAGAC AGAACTAAAC CAGTCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA GTTCCCGAGA AGAAAGAGGC TAAAGTCATT
    GAAAAGAAAG TTATACCAGC TCCCGTAAAG AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT
    CCTGAACTGA CAACAAAAAA AGCACCTGAG ATAACCAAAG AAAAACCGAA GCCAGTACCA
    AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA GCTAAACTAG CTCCTGAAAA GAAAGAGGTT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCGTTAAAGA AAGAGCCTGA GGTTCATAAG
    GAAAAAGCAC CTGAACCAAA ATCAGAACCT ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG
    GCAAAACCAG TTGCTAAAGA AAAGGAACCT GAAATAACCA AAGAAAAGCC AAAACCAGCA
    CCAAAGAAAG AAGCTGTAGT CATCAAAGAA AAAGCTGTAC CAGCCATCCT GAAGGAAGTG
    GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA
    GTTAAACCAG CTTCTATAAT GAAAGAGCCT GAAGTTGTGA AACAAAAGAC TCCTGAACCG
    AAACCCAAAA AAGAGCCTGA AGTTGTGAAA GAAAAGGCTC CTGAACCTAA ACCCAAAAAA
    GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA CCAGCACCTC AGAAGAAAGA AGTCATCAAA
    GAAAAAGTCA AACTTGCTCC TGTAAAGAAA GTGCCCGAAG TTGTCGAAGA AAAGGCACTT
    AAACCAGAAC CCAAAAAAGA TGAAGTTGGT CTTATTGGTC TGGACCTGGA GCTCCTAAAC
    TCCATCAACC AAAAACTGTC TCTCCTGGAC TTGCTAAGGA AGGACATTGG AGAAATGAAA
    AGCGATCTGG AGTCCACTCA GAATCAAATC ACACTTTTAC GGATGGACAA CAGAACGATC
    AAAGAGCCAG AGACTATTGG TGTGAAAGTA AAACCAGCAG TCAAGGAAAA AGCAAAAAGA
    GACCACGCTC TGATCAATGC TACTAGGACT GCACATATTA AAACAGAGCG TGAATCTCTA
    AAAAACATCA CGAAACCAGT GCCTGCAAAG AAAGCCAAAG AGGCTCCAAA GGTTAAGATT
    GCGCTGGAGC CTAAAAAGAA AGGAATCATC CCTGAGAAGG TCAAAGAAGT AAAGGAAACT
    TTACAGAAAG AACCTGAGTT GGTCAAAGAT AAAGTAGTTG AGCCAATAGA GAAGGAAATT
    GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG CCTGAAATCA CCAAAGATAT TCCAGAGGTG
    GAGGAAGAGG ACATTCCCTA CTTCCAGTGT TTCTTTGTGG ATGAGGATGA CACTCAGTAT
    CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323045.1
    CDS338..8230
    Translation

    Target ORF information:

    RefSeq Version XM_021467370.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X39, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCTGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCC AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA
    GTGAAACCAG CGCCAGAGAA GAAAGCACCC GAAATAACCA AAGAAAAACC AAAGCCAGCA
    GCAAAGAAAG TAGCTGAAGT CATCAAAGAC AGAACTAAAC CAGTCCCTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA GTTCCCGAGA AGAAAGAGGC TAAAGTCATT
    GAAAAGAAAG TTATACCAGC TCCCGTAAAG AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT
    CCTGAACTGA CAACAAAAAA AGCACCTGAG ATAACCAAAG AAAAACCGAA GCCAGTACCA
    AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA GCTAAACTAG CTCCTGAAAA GAAAGAGGTT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCGTTAAAGA AAGAGCCTGA GGTTCATAAG
    GAAAAAGCAC CTGAACCAAA ATCAGAACCT ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG
    GCAAAACCAG TTGCTAAAGA AAAGGAACCT GAAATAACCA AAGAAAAGCC AAAACCAGCA
    CCAAAGAAAG AAGCTGTAGT CATCAAAGAA AAAGCTGTAC CAGCCATCCT GAAGGAAGTG
    GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA
    GTTAAACCAG CTTCTATAAT GAAAGAGCCT GAAGTTGTGA AACAAAAGAC TCCTGAACCG
    AAACCCAAAA AAGAGCCTGA AGTTGTGAAA GAAAAGGCTC CTGAACCTAA ACCCAAAAAA
    GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA CCAGCACCTC AGAAGAAAGA AGTCATCAAA
    GAAAAAGTCA AACTTGCTCC TGTAAAGAAA GTGCCCGAAG TTGTCGAAGA AAAGGCACTT
    AAACCAGAAC CCAAAAAAGA TGAAGTTGGT CTTATTGGTC TGGACCTGGA GCTCCTAAAC
    TCCATCAACC AAAAACTGTC TCTCCTGGAC TTGCTAAGGA AGGACATTGG AGAAATGAAA
    AGCGATCTGG AGTCCACTCA GAATCAAATC ACACTTTTAC GGATGGACAA CAGAACGATC
    AAAGAGCCAG AGACTATTGG TGTGAAAGTA AAACCAGCAG TCAAGGAAAA AGCAAAAAGA
    GACCACGCTC TGATCAATGC TACTAGGACT GCACATATTA AAACAGAGCG TGAATCTCTA
    AAAAACATCA CGAAACCAGT GCCTGCAAAG AAAGCCAAAG AGGCTCCAAA GGTTAAGATT
    GCGCTGGAGC CTAAAAAGAA AGGAATCATC CCTGAGAAGG TCAAAGAAGT AAAGGAAACT
    TTACAGAAAG AACCTGAGTT GGTCAAAGAT AAAGTAGTTG AGCCAATAGA GAAGGAAATT
    GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG CCTGAAATCA CCAAAGATAT TCCAGAGGTG
    GAGGAAGAGG ACATTCCCTA CTTCCAGTGT TTCTTTGTGG ATGAGGATGA CACTCAGTAT
    CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149707
    Clone ID Related Accession (Same CDS sequence) XM_021467371.1
    Accession Version XM_021467371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X40
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT
    GAACTGACAA CAAAAAAAGC ACCTGAGATA ACCAAAGAAA AACCGAAGCC AGTACCAAAG
    AAAGAGGCTG AAGTCATTAA AGAGAAAGCT AAACTAGCTC CTGAAAAGAA AGAGGTTGAA
    GTCATCAAGG AGAAAGTTAA ACCAGCTCCG TTAAAGAAAG AGCCTGAGGT TCATAAGGAA
    AAAGCACCTG AACCAAAATC AGAACCTACA AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA
    AAACCAGTTG CTAAAGAAAA GGAACCTGAA ATAACCAAAG AAAAGCCAAA ACCAGCACCA
    AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA GCTGTACCAG CCATCCTGAA GGAAGTGGCT
    GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG AAGAAAGTGG CTGAAATTAA GGAGAAAGTT
    AAACCAGCTT CTATAATGAA AGAGCCTGAA GTTGTGAAAC AAAAGACTCC TGAACCGAAA
    CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA AAGGCTCCTG AACCTAAACC CAAAAAAGAG
    CCTGAAGTTG CTAAAGAGAA AGCCAAACCA GCACCTCAGA AGAAAGAAGT CATCAAAGAA
    AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG CCCGAAGTTG TCGAAGAAAA GGCACTTAAA
    CCAGAACCCA AAAAAGATGA AGTTGGTCTT ATTGGTCTGG ACCTGGAGCT CCTAAACTCC
    ATCAACCAAA AACTGTCTCT CCTGGACTTG CTAAGGAAGG ACATTGGAGA AATGAAAAGC
    GATCTGGAGT CCACTCAGAA TCAAATCACA CTTTTACGGA TGGACAACAG AACGATCAAA
    GAGCCAGAGA CTATTGGTGT GAAAGTAAAA CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC
    CACGCTCTGA TCAATGCTAC TAGGACTGCA CATATTAAAA CAGAGCGTGA ATCTCTAAAA
    AACATCACGA AACCAGTGCC TGCAAAGAAA GCCAAAGAGG CTCCAAAGGT TAAGATTGCG
    CTGGAGCCTA AAAAGAAAGG AATCATCCCT GAGAAGGTCA AAGAAGTAAA GGAAACTTTA
    CAGAAAGAAC CTGAGTTGGT CAAAGATAAA GTAGTTGAGC CAATAGAGAA GGAAATTGTT
    GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT GAAATCACCA AAGATATTCC AGAGGTGGAG
    GAAGAGGACA TTCCCTACTT CCAGTGTTTC TTTGTGGATG AGGATGACAC TCAGTATCCG
    TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323046.1
    CDS338..8227
    Translation

    Target ORF information:

    RefSeq Version XM_021467371.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT
    GAACTGACAA CAAAAAAAGC ACCTGAGATA ACCAAAGAAA AACCGAAGCC AGTACCAAAG
    AAAGAGGCTG AAGTCATTAA AGAGAAAGCT AAACTAGCTC CTGAAAAGAA AGAGGTTGAA
    GTCATCAAGG AGAAAGTTAA ACCAGCTCCG TTAAAGAAAG AGCCTGAGGT TCATAAGGAA
    AAAGCACCTG AACCAAAATC AGAACCTACA AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA
    AAACCAGTTG CTAAAGAAAA GGAACCTGAA ATAACCAAAG AAAAGCCAAA ACCAGCACCA
    AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA GCTGTACCAG CCATCCTGAA GGAAGTGGCT
    GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG AAGAAAGTGG CTGAAATTAA GGAGAAAGTT
    AAACCAGCTT CTATAATGAA AGAGCCTGAA GTTGTGAAAC AAAAGACTCC TGAACCGAAA
    CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA AAGGCTCCTG AACCTAAACC CAAAAAAGAG
    CCTGAAGTTG CTAAAGAGAA AGCCAAACCA GCACCTCAGA AGAAAGAAGT CATCAAAGAA
    AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG CCCGAAGTTG TCGAAGAAAA GGCACTTAAA
    CCAGAACCCA AAAAAGATGA AGTTGGTCTT ATTGGTCTGG ACCTGGAGCT CCTAAACTCC
    ATCAACCAAA AACTGTCTCT CCTGGACTTG CTAAGGAAGG ACATTGGAGA AATGAAAAGC
    GATCTGGAGT CCACTCAGAA TCAAATCACA CTTTTACGGA TGGACAACAG AACGATCAAA
    GAGCCAGAGA CTATTGGTGT GAAAGTAAAA CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC
    CACGCTCTGA TCAATGCTAC TAGGACTGCA CATATTAAAA CAGAGCGTGA ATCTCTAAAA
    AACATCACGA AACCAGTGCC TGCAAAGAAA GCCAAAGAGG CTCCAAAGGT TAAGATTGCG
    CTGGAGCCTA AAAAGAAAGG AATCATCCCT GAGAAGGTCA AAGAAGTAAA GGAAACTTTA
    CAGAAAGAAC CTGAGTTGGT CAAAGATAAA GTAGTTGAGC CAATAGAGAA GGAAATTGTT
    GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT GAAATCACCA AAGATATTCC AGAGGTGGAG
    GAAGAGGACA TTCCCTACTT CCAGTGTTTC TTTGTGGATG AGGATGACAC TCAGTATCCG
    TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149708
    Clone ID Related Accession (Same CDS sequence) XM_021467372.1
    Accession Version XM_021467372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X41
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGGAGGAAG CAAAACCAGC TCCTGTAAAA AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAAACAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCCCT
    GAGAAGAAAG AGGCTGAAGT CATCAAGGAG ATAGTTAAAC AAGCTCCTGT GAAGAAAGAG
    GCTGAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAAG TTAAAGCAGC ACCTCTGAAG AAAGAAGTCA AGGAGAAAGC AAAACCAGCT
    CCTGTAAAGA AAGAAGCTGA AGTCATCAAA CAAAAAGCCA AACCAGCTCC TGAGAAGAAA
    GAGGCCAAAG TCATCAAGGA GAAAGTTAAA CCGGCTCCTG TAAAGATAGA GGCTAAACCT
    GTAAAGGAGA TAACTAAACC AGCCCCTGAG AAGAAAGAGG CTGAAATAAT CAAGGAGAAG
    GTCAAATCAG CTCCTGTGAA GAAAGAAGTT GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT
    GAAAAGAAAG AGGCTGAAGT CATCAAGGAG AAAGTTAAAC CTGCTCCTGT GAGGAAAGAA
    GCTGAAGAAA TAAAGGAAAA AGCTGAAGCA GTTTATGAGA AAAAAGAGGC TGAAGTAGCC
    AAGGAGATAG TTAAACCAAC TCCTTTAAAG AAGGAGCCCG AAGTTGTGAA GGAAAAGGCT
    CCTGAATCAA AACCCCAAAA AGAGGCTGAA ATTATCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AGCCTGAAGT TGCAAAAGTA CCAAAACCCA AAAAAGAGGC TGAACTCGCC
    AAAGAGAAAG TTAAACCTGC ACCTGTAAAG AAAGAAGAAA AACCTAAGGC AGCACCAAAG
    AAAGAGGCTG AAGCTATTAA GGAGAAACGT ACACCAGCTC CTGAAAAGAA AGTGGTAGAA
    GTCATCAAGG AGAAAGTCAA ACCAGCCCCT GAGAAAAAAG CGGTTCTCAA GGAGAAAGTT
    AGACCTGCTC CTGAAAAGAA AGAGCCTGAA GTTGCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCCAAAAAG AGGTTGAAGT CATCAAAGAG AAAGCTAAAC CAGCCCTTAA GAAGAAAGAG
    CCTAAAGTCA AGGAAAAGGT TCAACCAGCT CCTATAAAGA AAGAGCCAGA GCTTCCCAAA
    GAAAAGGTGC CTGAACCAAC ACCAGAACCT GAGAGAGTTG AAGCTCCAAA AGAAAAGGCA
    AAAGTAATCC CTAGGGAAAA AGTAGCTGAG ATTACTAAAG AAAAACCAAA GCCAGCACCA
    AAGAAAGAGG CTGAAGTAAA GGAGAAAGCT ACACCTGCTC CTGTGCACAA AGAGGTTGAA
    GTGTTCAAGG AGAAAGTTAA ACAAGCTCCT GTAAAGAAAG AGCCAGAAGT TCCTAAAGAA
    AAGGCTCCTG AACCAAAACC TAAAAAGACT GAAGTCATCA AAGCGAAAGT AAAACCAGCC
    CCTGAGAAAA AAGAGGCTGA AGTGAAGGAG AAAATTAAAC CAGTCCCTGA GAAGAAAGAG
    GCTGAAGTCA TAAAGGAGAG AATTAAACCT GCTCCTGTAA AGAAAGAGCC TGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCCAAA AAAGAACCTG AAGTTATCAA AGAGAAAGTG
    AAACCAGCGC CAGAGAAGAA AGCACCCGAA ATAACCAAAG AAAAACCAAA GCCAGCAGCA
    AAGAAAGTAG CTGAAGTCAT CAAAGACAGA ACTAAACCAG TCCCTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAACC AAAAGCAGTT CCCGAGAAGA AAGAGGCTAA AGTCATTGAA
    AAGAAAGTTA TACCAGCTCC CGTAAAGAAA GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT
    GAACTGACAA CAAAAAAAGC ACCTGAGATA ACCAAAGAAA AACCGAAGCC AGTACCAAAG
    AAAGAGGCTG AAGTCATTAA AGAGAAAGCT AAACTAGCTC CTGAAAAGAA AGAGGTTGAA
    GTCATCAAGG AGAAAGTTAA ACCAGCTCCG TTAAAGAAAG AGCCTGAGGT TCATAAGGAA
    AAAGCACCTG AACCAAAATC AGAACCTACA AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA
    AAACCAGTTG CTAAAGAAAA GGAACCTGAA ATAACCAAAG AAAAGCCAAA ACCAGCACCA
    AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA GCTGTACCAG CCATCCTGAA GGAAGTGGCT
    GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG AAGAAAGTGG CTGAAATTAA GGAGAAAGTT
    AAACCAGCTT CTATAATGAA AGAGCCTGAA GTTGTGAAAC AAAAGACTCC TGAACCGAAA
    CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA AAGGCTCCTG AACCTAAACC CAAAAAAGAG
    CCTGAAGTTG CTAAAGAGAA AGCCAAACCA GCACCTCAGA AGAAAGAAGT CATCAAAGAA
    AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG CCCGAAGTTG TCGAAGAAAA GGCACTTAAA
    CCAGAACCCA AAAAAGATGA AGTTGGTCTT ATTGGTCTGG ACCTGGAGCT CCTAAACTCC
    ATCAACCAAA AACTGTCTCT CCTGGACTTG CTAAGGAAGG ACATTGGAGA AATGAAAAGC
    GATCTGGAGT CCACTCAGAA TCAAATCACA CTTTTACGGA TGGACAACAG AACGATCAAA
    GAGCCAGAGA CTATTGGTGT GAAAGTAAAA CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC
    CACGCTCTGA TCAATGCTAC TAGGACTGCA CATATTAAAA CAGAGCGTGA ATCTCTAAAA
    AACATCACGA AACCAGTGCC TGCAAAGAAA GCCAAAGAGG CTCCAAAGGT TAAGATTGCG
    CTGGAGCCTA AAAAGAAAGG AATCATCCCT GAGAAGGTCA AAGAAGTAAA GGAAACTTTA
    CAGAAAGAAC CTGAGTTGGT CAAAGATAAA GTAGTTGAGC CAATAGAGAA GGAAATTGTT
    GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT GAAATCACCA AAGATATTCC AGAGGTGGAG
    GAAGAGGACA TTCCCTACTT CCAGTGTTTC TTTGTGGATG AGGATGACAC TCAGTATCCG
    TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323047.1
    CDS338..8227
    Translation

    Target ORF information:

    RefSeq Version XM_021467372.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGGAGGAAG CAAAACCAGC TCCTGTAAAA AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAAACAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCCCT
    GAGAAGAAAG AGGCTGAAGT CATCAAGGAG ATAGTTAAAC AAGCTCCTGT GAAGAAAGAG
    GCTGAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAAG TTAAAGCAGC ACCTCTGAAG AAAGAAGTCA AGGAGAAAGC AAAACCAGCT
    CCTGTAAAGA AAGAAGCTGA AGTCATCAAA CAAAAAGCCA AACCAGCTCC TGAGAAGAAA
    GAGGCCAAAG TCATCAAGGA GAAAGTTAAA CCGGCTCCTG TAAAGATAGA GGCTAAACCT
    GTAAAGGAGA TAACTAAACC AGCCCCTGAG AAGAAAGAGG CTGAAATAAT CAAGGAGAAG
    GTCAAATCAG CTCCTGTGAA GAAAGAAGTT GAAGTCAAGG AGGAAGCAAA ACCAGCTCCT
    GAAAAGAAAG AGGCTGAAGT CATCAAGGAG AAAGTTAAAC CTGCTCCTGT GAGGAAAGAA
    GCTGAAGAAA TAAAGGAAAA AGCTGAAGCA GTTTATGAGA AAAAAGAGGC TGAAGTAGCC
    AAGGAGATAG TTAAACCAAC TCCTTTAAAG AAGGAGCCCG AAGTTGTGAA GGAAAAGGCT
    CCTGAATCAA AACCCCAAAA AGAGGCTGAA ATTATCAAAG AGAAAGTTAA ACCTGCACCT
    GTAAAGAAAG AGCCTGAAGT TGCAAAAGTA CCAAAACCCA AAAAAGAGGC TGAACTCGCC
    AAAGAGAAAG TTAAACCTGC ACCTGTAAAG AAAGAAGAAA AACCTAAGGC AGCACCAAAG
    AAAGAGGCTG AAGCTATTAA GGAGAAACGT ACACCAGCTC CTGAAAAGAA AGTGGTAGAA
    GTCATCAAGG AGAAAGTCAA ACCAGCCCCT GAGAAAAAAG CGGTTCTCAA GGAGAAAGTT
    AGACCTGCTC CTGAAAAGAA AGAGCCTGAA GTTGCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCCAAAAAG AGGTTGAAGT CATCAAAGAG AAAGCTAAAC CAGCCCTTAA GAAGAAAGAG
    CCTAAAGTCA AGGAAAAGGT TCAACCAGCT CCTATAAAGA AAGAGCCAGA GCTTCCCAAA
    GAAAAGGTGC CTGAACCAAC ACCAGAACCT GAGAGAGTTG AAGCTCCAAA AGAAAAGGCA
    AAAGTAATCC CTAGGGAAAA AGTAGCTGAG ATTACTAAAG AAAAACCAAA GCCAGCACCA
    AAGAAAGAGG CTGAAGTAAA GGAGAAAGCT ACACCTGCTC CTGTGCACAA AGAGGTTGAA
    GTGTTCAAGG AGAAAGTTAA ACAAGCTCCT GTAAAGAAAG AGCCAGAAGT TCCTAAAGAA
    AAGGCTCCTG AACCAAAACC TAAAAAGACT GAAGTCATCA AAGCGAAAGT AAAACCAGCC
    CCTGAGAAAA AAGAGGCTGA AGTGAAGGAG AAAATTAAAC CAGTCCCTGA GAAGAAAGAG
    GCTGAAGTCA TAAAGGAGAG AATTAAACCT GCTCCTGTAA AGAAAGAGCC TGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCCAAA AAAGAACCTG AAGTTATCAA AGAGAAAGTG
    AAACCAGCGC CAGAGAAGAA AGCACCCGAA ATAACCAAAG AAAAACCAAA GCCAGCAGCA
    AAGAAAGTAG CTGAAGTCAT CAAAGACAGA ACTAAACCAG TCCCTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAACC AAAAGCAGTT CCCGAGAAGA AAGAGGCTAA AGTCATTGAA
    AAGAAAGTTA TACCAGCTCC CGTAAAGAAA GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT
    GAACTGACAA CAAAAAAAGC ACCTGAGATA ACCAAAGAAA AACCGAAGCC AGTACCAAAG
    AAAGAGGCTG AAGTCATTAA AGAGAAAGCT AAACTAGCTC CTGAAAAGAA AGAGGTTGAA
    GTCATCAAGG AGAAAGTTAA ACCAGCTCCG TTAAAGAAAG AGCCTGAGGT TCATAAGGAA
    AAAGCACCTG AACCAAAATC AGAACCTACA AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA
    AAACCAGTTG CTAAAGAAAA GGAACCTGAA ATAACCAAAG AAAAGCCAAA ACCAGCACCA
    AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA GCTGTACCAG CCATCCTGAA GGAAGTGGCT
    GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG AAGAAAGTGG CTGAAATTAA GGAGAAAGTT
    AAACCAGCTT CTATAATGAA AGAGCCTGAA GTTGTGAAAC AAAAGACTCC TGAACCGAAA
    CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA AAGGCTCCTG AACCTAAACC CAAAAAAGAG
    CCTGAAGTTG CTAAAGAGAA AGCCAAACCA GCACCTCAGA AGAAAGAAGT CATCAAAGAA
    AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG CCCGAAGTTG TCGAAGAAAA GGCACTTAAA
    CCAGAACCCA AAAAAGATGA AGTTGGTCTT ATTGGTCTGG ACCTGGAGCT CCTAAACTCC
    ATCAACCAAA AACTGTCTCT CCTGGACTTG CTAAGGAAGG ACATTGGAGA AATGAAAAGC
    GATCTGGAGT CCACTCAGAA TCAAATCACA CTTTTACGGA TGGACAACAG AACGATCAAA
    GAGCCAGAGA CTATTGGTGT GAAAGTAAAA CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC
    CACGCTCTGA TCAATGCTAC TAGGACTGCA CATATTAAAA CAGAGCGTGA ATCTCTAAAA
    AACATCACGA AACCAGTGCC TGCAAAGAAA GCCAAAGAGG CTCCAAAGGT TAAGATTGCG
    CTGGAGCCTA AAAAGAAAGG AATCATCCCT GAGAAGGTCA AAGAAGTAAA GGAAACTTTA
    CAGAAAGAAC CTGAGTTGGT CAAAGATAAA GTAGTTGAGC CAATAGAGAA GGAAATTGTT
    GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT GAAATCACCA AAGATATTCC AGAGGTGGAG
    GAAGAGGACA TTCCCTACTT CCAGTGTTTC TTTGTGGATG AGGATGACAC TCAGTATCCG
    TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149709
    Clone ID Related Accession (Same CDS sequence) XM_021467373.1
    Accession Version XM_021467373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT CCTGGAGGTA
    ACCATTATTT ATCCAGCAGA TATGGCAAGT GAAGCATTTT TGCAAATTTT AAATGTGATC
    AAGAACACTT CATCAGCCAT ATTTGGGTTT CTTGAAGCTT GGTTCCCCAA AATGAGCACT
    CGTCCTACAG AGCTGGTTGA AAAGGTTGCA GAGGAGGCCA CTGATGTAAA GACCACCGTC
    TCTAATTATC TGTCCAGTCT GTTTGTGAGG GATGAAGGTG GTATTCCTGA CGTCACTTTT
    GATCCCATGA AAGTTGTCTC AGACACCGTT GAGGAGTTTG CAGACAGAAG AGATATGTTA
    TTAGCTTATC TCTCAAACAT GCTCATGGAA GACAAAGTTT CAGATGAAAC ACCACAGGTG
    ATAAGGCGAA AAGGAGAATT TCTGCCCCCT ATGGAAAAAG TTGCAGAAGT ACTAGGAAAA
    ACTCAGGACC CCATCTACGC TGCCTACATC GCTGTGAGAT CCACAGCTGA GACAGACGGC
    GATGAACTGG AAATAAAGGG GGCTGAGGTG GACTCTGCTG AAGATATCTT GGAAGAACAA
    AAAGTTGAAA AGACAGTGGA CGGCTCTGAA AATGATGCAG TTGCCTCTGT TGAGGAGGAA
    AAAGCAGTTG TTGATATTGT AGATGAGGAA TCTTCTCATA CGCCTGAAGT CACAGATGAA
    GAAATGGCAA AAGAAAAAGA TGAAGACACT TCTGATTCAA CTGAAATGAC TTCTGATGTG
    CAAGACATTG ATGATACCAT AGATTCTGAG ACTGAAGAAA TTCCTGAAAT GGTTGAAAGT
    GTTTCAGAGG TTGGAGAAGA GGAACCTTTT GTAATTGAAA AGGTTGAAGA CACCTCTGAT
    TCGAAAGAAA TCACTTCTAA TGCCGCAGAT GTGCAAGATG TTGATGATAA TTCTGATGTT
    GATACTGAAG AAATTCCTGA ATTGGTTGGA GGTGTTTCAG ATGTTGGTGA AGAGGAACCT
    TCTGAAGTCA TAGATGAAGA AATTGCAACT GAAAAGCTTG AAGACGCCTC TGATTCAACT
    GGATTGATTC CCAGTGCTGA AAATGTGAAA GACACTGACG ATATCTTAGA AGTTGACGCA
    GAGGTGAATG AAGTTGAAAC AGTTGAAGAC ATTTCAGAAG TTACAAATGA GGAAACTTCT
    GATGTGCCAG AAGTCATAGA TGAGGAAAAG GTTGAAAAGT CTGATGATAT CTTTCATTTT
    GCAGAGGACA AACCAACTGA ATTGATTTCT GATGCTCCAG ATGTAACAGA AGACATTGAA
    ATCTCAATCT CTGCAGTTTC AGAGGATAAT GAAACAATTC AAGAAGCTGA TATGACAGAT
    GTCAAAGATG AGGAAATGGA AGCTGAAAAG GTTGATGAAA TCTCCTATTT TGTAGAGGAT
    AAATCAACTG AAATTACTTC TGATGCTACA GATGTGAAAG AAGACACTGA TGGCTTTACA
    GTTACAGAGG AGAAAGGAAC AATTGAAAGC CTTCAAGAAG CAGCAATGAA AATGTCGGGA
    AATGCAGATG ATGAAATCCC CAATGTCACA GAGATGGTTG AAGAACCTGA TACTTCTGAT
    GTTATGGATG AGCTAGACGA ACCTGAAAAA GATGAAGATG TAATCAGTGT CACCAATGAA
    AGGGTTACAA CTGCACAAGA TGTCATATTT TCAAAGGAAA AAATGGGAGC AGCAGAACTG
    GATGATCATG ACAAACTCAG GGCTAAGGAT GATTCAGAAG TGGATCACAT GGAGAAGGAA
    ATTGCAGATG CTGAAAATGA CCTTCAGAAT GAGATAGATA AAGATGAGTT TAAGGTTGAA
    GATGCATCTC ATCAAACTAT TTTGGGAAGC ACTGCTGCTG AAGAAGACCA AGATGATGAT
    GATGATGATG AAGAACCTGT CCTTCTTACT GTCAAACATG AGCTCACAGA CGGTGATGAT
    GAAAATAATA ATAACAACGA CAGAAAGAGA GATGCCAGAA CTAAAGGTCT GATCAATAGA
    AAAACACCAA AGAGCCATTC CACTGAAACT GATGAACAAA AGGCGCACAA GGATCTTCAT
    AAAGAGTCTG CTAAAGTTCT TGAGCAAGTG AAAGAAGCCA AAGAGAAAAA AGCTAGAGAG
    GAGATATACA AAATCATTCA AGAAATGAGG GAGGAGAAGG CTGAAGGTGT GGAGGAAGAG
    ATCGTGTTAC AACAGATAAA CATAACAGTA GAAAAGGTCA AGAAAGAAGA AACTGTCAAA
    AAGGAGGAAA AGGCTAAAAG AAAAGAAAAG GCTCATAAAC CAAAGGATGC AAAGATTGCC
    AAAGACAAAC CTAAAGCAGT CCCAGTAAAG CAAGAGGATG AAGTAGCCAA GAAAGAAACC
    AAACCAGCAC CATCTAAGAA AGAGGCTAAA ATTGTGAAGG AGAAACCCAA AGCACATCCT
    GCAAAGAAAG AAAAAGCTAA AGATATTAAG GAAAAGGCAA AATCTGGTCA AAAAGAAAAA
    GTTGCTGAGG CTGTCAAGGA AAAATCGAAG CCAGTAAAGA AAGAAGCTGA AAAAATTGAT
    GCCAAAGTCA AACCAGCAGC AGAAAAGAAA GTGGATGAAG CCCCCAAAGA GAAACCTAAA
    GTGAAGAAAG AGGCTGAAGT AATCAAAAAG ATTAAACTAG AGCCTGAGAG GAAAGAAGCT
    AAAACTCCCA AGGCCAAAAT TGAACCAGCT GGTTTGAAGA AAGATGCTGA AGCTGCTAAG
    GAGAAAGCAA AACCTGCTCC AGTCAAGAAA GGGCCTGAGG TTTCCAGAGA AAAAGCCAAA
    CCAGAGCCCG CAAAAAAAGA GCTTGAAGCG GTCAAAGAGA AAGCAAAACC AGCACCAGCT
    AAAAAAGATG AAGTCAAAGA GAAAGAAAAA CCAGCTCCTG TAAAGAAAGC TGAAGAAATT
    AAAAAGGCAA AAGCAGCTCC TTCAAGGAAA GAAGCTGTAA AGGAAAAACC CAAACCTGTT
    TCCGAAAAGA AAGAACCTGC TGTTGCAGTT GAAAAAGCCA AGCCAAAATC TACAAAGAAA
    GAAGCTGAAG TCGAGGAAAA AGTGAAACCT GCTCCTGAGA AGAAAGAAGC TGAAGTCATC
    ATGGTGAAAA CTAAACCAGT TCTTAAAAAG AAAGAGGCTG AAGTAATCAA AGAGGTTAAA
    CCAGCCCTTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC AGCTCCTGTA
    AAGAAAGAGC CTGAAGTTGT TAAAGAAAAG GAACCTGAAC CAAAACCCAA AAGAGAGCCA
    GAAATTATTA AGGAGAAATC AGCACCACCA AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT
    CCTGAAAAGA AAGCACCTGA AATAACCAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGCTG TAAAAGAGAA AGCTAAACCA GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG
    AAAACGAAAC CAGCCCCTGA GAAAGAGACT GAAATAATCA AGGATAAAAT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAACCA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CTCTGAGAAG AAAGAGGCTG AAATAATTAA GGAGAAAGTT
    AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA GTCAAGGAGG AAGCAAAACC AGCTCCTGTA
    AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA GAAAAAACAG CTCCTGAGAA GAAAGAGGCT
    GAAGCTGTAA AGGAGAAAAC GAAACCAACC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGATAGTTA AACAAGCTCC TGTGAAGAAA GAGGCTGAAC CTGTAAAGGA GATAACTAAA
    CCAGCCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323048.1
    CDS338..8200
    Translation

    Target ORF information:

    RefSeq Version XM_021467373.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT CCTGGAGGTA
    ACCATTATTT ATCCAGCAGA TATGGCAAGT GAAGCATTTT TGCAAATTTT AAATGTGATC
    AAGAACACTT CATCAGCCAT ATTTGGGTTT CTTGAAGCTT GGTTCCCCAA AATGAGCACT
    CGTCCTACAG AGCTGGTTGA AAAGGTTGCA GAGGAGGCCA CTGATGTAAA GACCACCGTC
    TCTAATTATC TGTCCAGTCT GTTTGTGAGG GATGAAGGTG GTATTCCTGA CGTCACTTTT
    GATCCCATGA AAGTTGTCTC AGACACCGTT GAGGAGTTTG CAGACAGAAG AGATATGTTA
    TTAGCTTATC TCTCAAACAT GCTCATGGAA GACAAAGTTT CAGATGAAAC ACCACAGGTG
    ATAAGGCGAA AAGGAGAATT TCTGCCCCCT ATGGAAAAAG TTGCAGAAGT ACTAGGAAAA
    ACTCAGGACC CCATCTACGC TGCCTACATC GCTGTGAGAT CCACAGCTGA GACAGACGGC
    GATGAACTGG AAATAAAGGG GGCTGAGGTG GACTCTGCTG AAGATATCTT GGAAGAACAA
    AAAGTTGAAA AGACAGTGGA CGGCTCTGAA AATGATGCAG TTGCCTCTGT TGAGGAGGAA
    AAAGCAGTTG TTGATATTGT AGATGAGGAA TCTTCTCATA CGCCTGAAGT CACAGATGAA
    GAAATGGCAA AAGAAAAAGA TGAAGACACT TCTGATTCAA CTGAAATGAC TTCTGATGTG
    CAAGACATTG ATGATACCAT AGATTCTGAG ACTGAAGAAA TTCCTGAAAT GGTTGAAAGT
    GTTTCAGAGG TTGGAGAAGA GGAACCTTTT GTAATTGAAA AGGTTGAAGA CACCTCTGAT
    TCGAAAGAAA TCACTTCTAA TGCCGCAGAT GTGCAAGATG TTGATGATAA TTCTGATGTT
    GATACTGAAG AAATTCCTGA ATTGGTTGGA GGTGTTTCAG ATGTTGGTGA AGAGGAACCT
    TCTGAAGTCA TAGATGAAGA AATTGCAACT GAAAAGCTTG AAGACGCCTC TGATTCAACT
    GGATTGATTC CCAGTGCTGA AAATGTGAAA GACACTGACG ATATCTTAGA AGTTGACGCA
    GAGGTGAATG AAGTTGAAAC AGTTGAAGAC ATTTCAGAAG TTACAAATGA GGAAACTTCT
    GATGTGCCAG AAGTCATAGA TGAGGAAAAG GTTGAAAAGT CTGATGATAT CTTTCATTTT
    GCAGAGGACA AACCAACTGA ATTGATTTCT GATGCTCCAG ATGTAACAGA AGACATTGAA
    ATCTCAATCT CTGCAGTTTC AGAGGATAAT GAAACAATTC AAGAAGCTGA TATGACAGAT
    GTCAAAGATG AGGAAATGGA AGCTGAAAAG GTTGATGAAA TCTCCTATTT TGTAGAGGAT
    AAATCAACTG AAATTACTTC TGATGCTACA GATGTGAAAG AAGACACTGA TGGCTTTACA
    GTTACAGAGG AGAAAGGAAC AATTGAAAGC CTTCAAGAAG CAGCAATGAA AATGTCGGGA
    AATGCAGATG ATGAAATCCC CAATGTCACA GAGATGGTTG AAGAACCTGA TACTTCTGAT
    GTTATGGATG AGCTAGACGA ACCTGAAAAA GATGAAGATG TAATCAGTGT CACCAATGAA
    AGGGTTACAA CTGCACAAGA TGTCATATTT TCAAAGGAAA AAATGGGAGC AGCAGAACTG
    GATGATCATG ACAAACTCAG GGCTAAGGAT GATTCAGAAG TGGATCACAT GGAGAAGGAA
    ATTGCAGATG CTGAAAATGA CCTTCAGAAT GAGATAGATA AAGATGAGTT TAAGGTTGAA
    GATGCATCTC ATCAAACTAT TTTGGGAAGC ACTGCTGCTG AAGAAGACCA AGATGATGAT
    GATGATGATG AAGAACCTGT CCTTCTTACT GTCAAACATG AGCTCACAGA CGGTGATGAT
    GAAAATAATA ATAACAACGA CAGAAAGAGA GATGCCAGAA CTAAAGGTCT GATCAATAGA
    AAAACACCAA AGAGCCATTC CACTGAAACT GATGAACAAA AGGCGCACAA GGATCTTCAT
    AAAGAGTCTG CTAAAGTTCT TGAGCAAGTG AAAGAAGCCA AAGAGAAAAA AGCTAGAGAG
    GAGATATACA AAATCATTCA AGAAATGAGG GAGGAGAAGG CTGAAGGTGT GGAGGAAGAG
    ATCGTGTTAC AACAGATAAA CATAACAGTA GAAAAGGTCA AGAAAGAAGA AACTGTCAAA
    AAGGAGGAAA AGGCTAAAAG AAAAGAAAAG GCTCATAAAC CAAAGGATGC AAAGATTGCC
    AAAGACAAAC CTAAAGCAGT CCCAGTAAAG CAAGAGGATG AAGTAGCCAA GAAAGAAACC
    AAACCAGCAC CATCTAAGAA AGAGGCTAAA ATTGTGAAGG AGAAACCCAA AGCACATCCT
    GCAAAGAAAG AAAAAGCTAA AGATATTAAG GAAAAGGCAA AATCTGGTCA AAAAGAAAAA
    GTTGCTGAGG CTGTCAAGGA AAAATCGAAG CCAGTAAAGA AAGAAGCTGA AAAAATTGAT
    GCCAAAGTCA AACCAGCAGC AGAAAAGAAA GTGGATGAAG CCCCCAAAGA GAAACCTAAA
    GTGAAGAAAG AGGCTGAAGT AATCAAAAAG ATTAAACTAG AGCCTGAGAG GAAAGAAGCT
    AAAACTCCCA AGGCCAAAAT TGAACCAGCT GGTTTGAAGA AAGATGCTGA AGCTGCTAAG
    GAGAAAGCAA AACCTGCTCC AGTCAAGAAA GGGCCTGAGG TTTCCAGAGA AAAAGCCAAA
    CCAGAGCCCG CAAAAAAAGA GCTTGAAGCG GTCAAAGAGA AAGCAAAACC AGCACCAGCT
    AAAAAAGATG AAGTCAAAGA GAAAGAAAAA CCAGCTCCTG TAAAGAAAGC TGAAGAAATT
    AAAAAGGCAA AAGCAGCTCC TTCAAGGAAA GAAGCTGTAA AGGAAAAACC CAAACCTGTT
    TCCGAAAAGA AAGAACCTGC TGTTGCAGTT GAAAAAGCCA AGCCAAAATC TACAAAGAAA
    GAAGCTGAAG TCGAGGAAAA AGTGAAACCT GCTCCTGAGA AGAAAGAAGC TGAAGTCATC
    ATGGTGAAAA CTAAACCAGT TCTTAAAAAG AAAGAGGCTG AAGTAATCAA AGAGGTTAAA
    CCAGCCCTTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC AGCTCCTGTA
    AAGAAAGAGC CTGAAGTTGT TAAAGAAAAG GAACCTGAAC CAAAACCCAA AAGAGAGCCA
    GAAATTATTA AGGAGAAATC AGCACCACCA AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT
    CCTGAAAAGA AAGCACCTGA AATAACCAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGCTG TAAAAGAGAA AGCTAAACCA GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG
    AAAACGAAAC CAGCCCCTGA GAAAGAGACT GAAATAATCA AGGATAAAAT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAACCA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CTCTGAGAAG AAAGAGGCTG AAATAATTAA GGAGAAAGTT
    AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA GTCAAGGAGG AAGCAAAACC AGCTCCTGTA
    AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA GAAAAAACAG CTCCTGAGAA GAAAGAGGCT
    GAAGCTGTAA AGGAGAAAAC GAAACCAACC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGATAGTTA AACAAGCTCC TGTGAAGAAA GAGGCTGAAC CTGTAAAGGA GATAACTAAA
    CCAGCCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149710
    Clone ID Related Accession (Same CDS sequence) XM_021467374.1
    Accession Version XM_021467374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X43
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAGGT CAAATCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG
    GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG GCTGAAGTCA TCAAGGAGAA AGTTAAACCT
    GCTCCTGTGA GGAAAGAAGC TGAAGAAATA AAGGAAAAAG CTGAAGCAGT TTATGAGAAA
    AAAGAGGCTG AAGTAGCCAA GGAGATAGTT AAACCAACTC CTTTAAAGAA GGAGCCCGAA
    GTTGTGAAGG AAAAGGCTCC TGAATCAAAA CCCCAAAAAG AGGCTGAAAT TATCAAAGAG
    AAAGTTAAAC CTGCACCTGT AAAGAAAGAG CCTGAAGTTG CAAAAGTACC AAAACCCAAA
    AAAGAGGCTG AACTCGCCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAAGAAAAA
    CCTAAGGCAG CACCAAAGAA AGAGGCTGAA GCTATTAAGG AGAAACGTAC ACCAGCTCCT
    GAAAAGAAAG TGGTAGAAGT CATCAAGGAG AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG
    GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA
    AAGGCTCCTG AACCAAAACC CCAAAAAGAG GTTGAAGTCA TCAAAGAGAA AGCTAAACCA
    GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG GAAAAGGTTC AACCAGCTCC TATAAAGAAA
    GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT GAACCAACAC CAGAACCTGA GAGAGTTGAA
    GCTCCAAAAG AAAAGGCAAA AGTAATCCCT AGGGAAAAAG TAGCTGAGAT TACTAAAGAA
    AAACCAAAGC CAGCACCAAA GAAAGAGGCT GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT
    GTGCACAAAG AGGTTGAAGT GTTCAAGGAG AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG
    CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA CCAAAACCTA AAAAGACTGA AGTCATCAAA
    GCGAAAGTAA AACCAGCCCC TGAGAAAAAA GAGGCTGAAG TGAAGGAGAA AATTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATA AAGGAGAGAA TTAAACCTGC TCCTGTAAAG
    AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCCAAAAA AGAACCTGAA
    GTTATCAAAG AGAAAGTGAA ACCAGCGCCA GAGAAGAAAG CACCCGAAAT AACCAAAGAA
    AAACCAAAGC CAGCAGCAAA GAAAGTAGCT GAAGTCATCA AAGACAGAAC TAAACCAGTC
    CCTGAGAAGA AAGAGGCTGA AGTCATCAAG GAGAAACCAA AAGCAGTTCC CGAGAAGAAA
    GAGGCTAAAG TCATTGAAAA GAAAGTTATA CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT
    GTTAAGGAAA AGGCTCCTGA ACTGACAACA AAAAAAGCAC CTGAGATAAC CAAAGAAAAA
    CCGAAGCCAG TACCAAAGAA AGAGGCTGAA GTCATTAAAG AGAAAGCTAA ACTAGCTCCT
    GAAAAGAAAG AGGTTGAAGT CATCAAGGAG AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG
    CCTGAGGTTC ATAAGGAAAA AGCACCTGAA CCAAAATCAG AACCTACAAA AAAAGTTGAG
    GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT AAAGAAAAGG AACCTGAAAT AACCAAAGAA
    AAGCCAAAAC CAGCACCAAA GAAAGAAGCT GTAGTCATCA AAGAAAAAGC TGTACCAGCC
    ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT
    GAAATTAAGG AGAAAGTTAA ACCAGCTTCT ATAATGAAAG AGCCTGAAGT TGTGAAACAA
    AAGACTCCTG AACCGAAACC CAAAAAAGAG CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA
    CCTAAACCCA AAAAAGAGCC TGAAGTTGCT AAAGAGAAAG CCAAACCAGC ACCTCAGAAG
    AAAGAAGTCA TCAAAGAAAA AGTCAAACTT GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC
    GAAGAAAAGG CACTTAAACC AGAACCCAAA AAAGATGAAG TTGGTCTTAT TGGTCTGGAC
    CTGGAGCTCC TAAACTCCAT CAACCAAAAA CTGTCTCTCC TGGACTTGCT AAGGAAGGAC
    ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC ACTCAGAATC AAATCACACT TTTACGGATG
    GACAACAGAA CGATCAAAGA GCCAGAGACT ATTGGTGTGA AAGTAAAACC AGCAGTCAAG
    GAAAAAGCAA AAAGAGACCA CGCTCTGATC AATGCTACTA GGACTGCACA TATTAAAACA
    GAGCGTGAAT CTCTAAAAAA CATCACGAAA CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT
    CCAAAGGTTA AGATTGCGCT GGAGCCTAAA AAGAAAGGAA TCATCCCTGA GAAGGTCAAA
    GAAGTAAAGG AAACTTTACA GAAAGAACCT GAGTTGGTCA AAGATAAAGT AGTTGAGCCA
    ATAGAGAAGG AAATTGTTGT TGAGAAGGAA GTCAAAGGAG AAGAGCCTGA AATCACCAAA
    GATATTCCAG AGGTGGAGGA AGAGGACATT CCCTACTTCC AGTGTTTCTT TGTGGATGAG
    GATGACACTC AGTATCCGTT CTTCCCTTTC TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323049.1
    CDS338..8185
    Translation

    Target ORF information:

    RefSeq Version XM_021467374.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAGGT CAAATCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG
    GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG GCTGAAGTCA TCAAGGAGAA AGTTAAACCT
    GCTCCTGTGA GGAAAGAAGC TGAAGAAATA AAGGAAAAAG CTGAAGCAGT TTATGAGAAA
    AAAGAGGCTG AAGTAGCCAA GGAGATAGTT AAACCAACTC CTTTAAAGAA GGAGCCCGAA
    GTTGTGAAGG AAAAGGCTCC TGAATCAAAA CCCCAAAAAG AGGCTGAAAT TATCAAAGAG
    AAAGTTAAAC CTGCACCTGT AAAGAAAGAG CCTGAAGTTG CAAAAGTACC AAAACCCAAA
    AAAGAGGCTG AACTCGCCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAAGAAAAA
    CCTAAGGCAG CACCAAAGAA AGAGGCTGAA GCTATTAAGG AGAAACGTAC ACCAGCTCCT
    GAAAAGAAAG TGGTAGAAGT CATCAAGGAG AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG
    GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT GAAAAGAAAG AGCCTGAAGT TGCTAAAGAA
    AAGGCTCCTG AACCAAAACC CCAAAAAGAG GTTGAAGTCA TCAAAGAGAA AGCTAAACCA
    GCCCTTAAGA AGAAAGAGCC TAAAGTCAAG GAAAAGGTTC AACCAGCTCC TATAAAGAAA
    GAGCCAGAGC TTCCCAAAGA AAAGGTGCCT GAACCAACAC CAGAACCTGA GAGAGTTGAA
    GCTCCAAAAG AAAAGGCAAA AGTAATCCCT AGGGAAAAAG TAGCTGAGAT TACTAAAGAA
    AAACCAAAGC CAGCACCAAA GAAAGAGGCT GAAGTAAAGG AGAAAGCTAC ACCTGCTCCT
    GTGCACAAAG AGGTTGAAGT GTTCAAGGAG AAAGTTAAAC AAGCTCCTGT AAAGAAAGAG
    CCAGAAGTTC CTAAAGAAAA GGCTCCTGAA CCAAAACCTA AAAAGACTGA AGTCATCAAA
    GCGAAAGTAA AACCAGCCCC TGAGAAAAAA GAGGCTGAAG TGAAGGAGAA AATTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATA AAGGAGAGAA TTAAACCTGC TCCTGTAAAG
    AAAGAGCCTG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCCAAAAA AGAACCTGAA
    GTTATCAAAG AGAAAGTGAA ACCAGCGCCA GAGAAGAAAG CACCCGAAAT AACCAAAGAA
    AAACCAAAGC CAGCAGCAAA GAAAGTAGCT GAAGTCATCA AAGACAGAAC TAAACCAGTC
    CCTGAGAAGA AAGAGGCTGA AGTCATCAAG GAGAAACCAA AAGCAGTTCC CGAGAAGAAA
    GAGGCTAAAG TCATTGAAAA GAAAGTTATA CCAGCTCCCG TAAAGAAAGA GCCTGAAGTT
    GTTAAGGAAA AGGCTCCTGA ACTGACAACA AAAAAAGCAC CTGAGATAAC CAAAGAAAAA
    CCGAAGCCAG TACCAAAGAA AGAGGCTGAA GTCATTAAAG AGAAAGCTAA ACTAGCTCCT
    GAAAAGAAAG AGGTTGAAGT CATCAAGGAG AAAGTTAAAC CAGCTCCGTT AAAGAAAGAG
    CCTGAGGTTC ATAAGGAAAA AGCACCTGAA CCAAAATCAG AACCTACAAA AAAAGTTGAG
    GTTCCAAAGG AAAAGGCAAA ACCAGTTGCT AAAGAAAAGG AACCTGAAAT AACCAAAGAA
    AAGCCAAAAC CAGCACCAAA GAAAGAAGCT GTAGTCATCA AAGAAAAAGC TGTACCAGCC
    ATCCTGAAGG AAGTGGCTGA AGTAAAGAAA GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT
    GAAATTAAGG AGAAAGTTAA ACCAGCTTCT ATAATGAAAG AGCCTGAAGT TGTGAAACAA
    AAGACTCCTG AACCGAAACC CAAAAAAGAG CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA
    CCTAAACCCA AAAAAGAGCC TGAAGTTGCT AAAGAGAAAG CCAAACCAGC ACCTCAGAAG
    AAAGAAGTCA TCAAAGAAAA AGTCAAACTT GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC
    GAAGAAAAGG CACTTAAACC AGAACCCAAA AAAGATGAAG TTGGTCTTAT TGGTCTGGAC
    CTGGAGCTCC TAAACTCCAT CAACCAAAAA CTGTCTCTCC TGGACTTGCT AAGGAAGGAC
    ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC ACTCAGAATC AAATCACACT TTTACGGATG
    GACAACAGAA CGATCAAAGA GCCAGAGACT ATTGGTGTGA AAGTAAAACC AGCAGTCAAG
    GAAAAAGCAA AAAGAGACCA CGCTCTGATC AATGCTACTA GGACTGCACA TATTAAAACA
    GAGCGTGAAT CTCTAAAAAA CATCACGAAA CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT
    CCAAAGGTTA AGATTGCGCT GGAGCCTAAA AAGAAAGGAA TCATCCCTGA GAAGGTCAAA
    GAAGTAAAGG AAACTTTACA GAAAGAACCT GAGTTGGTCA AAGATAAAGT AGTTGAGCCA
    ATAGAGAAGG AAATTGTTGT TGAGAAGGAA GTCAAAGGAG AAGAGCCTGA AATCACCAAA
    GATATTCCAG AGGTGGAGGA AGAGGACATT CCCTACTTCC AGTGTTTCTT TGTGGATGAG
    GATGACACTC AGTATCCGTT CTTCCCTTTC TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149711
    Clone ID Related Accession (Same CDS sequence) XM_021467375.1
    Accession Version XM_021467375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X44
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT CAAGGAGAAA CCAAAAGCAG TTCCCGAGAA GAAAGAGGCT
    AAAGTCATTG AAAAGAAAGT TATACCAGCT CCCGTAAAGA AAGAGCCTGA AGTTGTTAAG
    GAAAAGGCTC CTGAACTGAC AACAAAAAAA GCACCTGAGA TAACCAAAGA AAAACCGAAG
    CCAGTACCAA AGAAAGAGGC TGAAGTCATT AAAGAGAAAG CTAAACTAGC TCCTGAAAAG
    AAAGAGGTTG AAGTCATCAA GGAGAAAGTT AAACCAGCTC CGTTAAAGAA AGAGCCTGAG
    GTTCATAAGG AAAAAGCACC TGAACCAAAA TCAGAACCTA CAAAAAAAGT TGAGGTTCCA
    AAGGAAAAGG CAAAACCAGT TGCTAAAGAA AAGGAACCTG AAATAACCAA AGAAAAGCCA
    AAACCAGCAC CAAAGAAAGA AGCTGTAGTC ATCAAAGAAA AAGCTGTACC AGCCATCCTG
    AAGGAAGTGG CTGAAGTAAA GAAAGTTAAA GCAGCTCCTG AGAAGAAAGT GGCTGAAATT
    AAGGAGAAAG TTAAACCAGC TTCTATAATG AAAGAGCCTG AAGTTGTGAA ACAAAAGACT
    CCTGAACCGA AACCCAAAAA AGAGCCTGAA GTTGTGAAAG AAAAGGCTCC TGAACCTAAA
    CCCAAAAAAG AGCCTGAAGT TGCTAAAGAG AAAGCCAAAC CAGCACCTCA GAAGAAAGAA
    GTCATCAAAG AAAAAGTCAA ACTTGCTCCT GTAAAGAAAG TGCCCGAAGT TGTCGAAGAA
    AAGGCACTTA AACCAGAACC CAAAAAAGAT GAAGTTGGTC TTATTGGTCT GGACCTGGAG
    CTCCTAAACT CCATCAACCA AAAACTGTCT CTCCTGGACT TGCTAAGGAA GGACATTGGA
    GAAATGAAAA GCGATCTGGA GTCCACTCAG AATCAAATCA CACTTTTACG GATGGACAAC
    AGAACGATCA AAGAGCCAGA GACTATTGGT GTGAAAGTAA AACCAGCAGT CAAGGAAAAA
    GCAAAAAGAG ACCACGCTCT GATCAATGCT ACTAGGACTG CACATATTAA AACAGAGCGT
    GAATCTCTAA AAAACATCAC GAAACCAGTG CCTGCAAAGA AAGCCAAAGA GGCTCCAAAG
    GTTAAGATTG CGCTGGAGCC TAAAAAGAAA GGAATCATCC CTGAGAAGGT CAAAGAAGTA
    AAGGAAACTT TACAGAAAGA ACCTGAGTTG GTCAAAGATA AAGTAGTTGA GCCAATAGAG
    AAGGAAATTG TTGTTGAGAA GGAAGTCAAA GGAGAAGAGC CTGAAATCAC CAAAGATATT
    CCAGAGGTGG AGGAAGAGGA CATTCCCTAC TTCCAGTGTT TCTTTGTGGA TGAGGATGAC
    ACTCAGTATC CGTTCTTCCC TTTCTCTCCA CCGCTTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323050.1
    CDS338..8179
    Translation

    Target ORF information:

    RefSeq Version XM_021467375.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X44, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT CAAGGAGAAA CCAAAAGCAG TTCCCGAGAA GAAAGAGGCT
    AAAGTCATTG AAAAGAAAGT TATACCAGCT CCCGTAAAGA AAGAGCCTGA AGTTGTTAAG
    GAAAAGGCTC CTGAACTGAC AACAAAAAAA GCACCTGAGA TAACCAAAGA AAAACCGAAG
    CCAGTACCAA AGAAAGAGGC TGAAGTCATT AAAGAGAAAG CTAAACTAGC TCCTGAAAAG
    AAAGAGGTTG AAGTCATCAA GGAGAAAGTT AAACCAGCTC CGTTAAAGAA AGAGCCTGAG
    GTTCATAAGG AAAAAGCACC TGAACCAAAA TCAGAACCTA CAAAAAAAGT TGAGGTTCCA
    AAGGAAAAGG CAAAACCAGT TGCTAAAGAA AAGGAACCTG AAATAACCAA AGAAAAGCCA
    AAACCAGCAC CAAAGAAAGA AGCTGTAGTC ATCAAAGAAA AAGCTGTACC AGCCATCCTG
    AAGGAAGTGG CTGAAGTAAA GAAAGTTAAA GCAGCTCCTG AGAAGAAAGT GGCTGAAATT
    AAGGAGAAAG TTAAACCAGC TTCTATAATG AAAGAGCCTG AAGTTGTGAA ACAAAAGACT
    CCTGAACCGA AACCCAAAAA AGAGCCTGAA GTTGTGAAAG AAAAGGCTCC TGAACCTAAA
    CCCAAAAAAG AGCCTGAAGT TGCTAAAGAG AAAGCCAAAC CAGCACCTCA GAAGAAAGAA
    GTCATCAAAG AAAAAGTCAA ACTTGCTCCT GTAAAGAAAG TGCCCGAAGT TGTCGAAGAA
    AAGGCACTTA AACCAGAACC CAAAAAAGAT GAAGTTGGTC TTATTGGTCT GGACCTGGAG
    CTCCTAAACT CCATCAACCA AAAACTGTCT CTCCTGGACT TGCTAAGGAA GGACATTGGA
    GAAATGAAAA GCGATCTGGA GTCCACTCAG AATCAAATCA CACTTTTACG GATGGACAAC
    AGAACGATCA AAGAGCCAGA GACTATTGGT GTGAAAGTAA AACCAGCAGT CAAGGAAAAA
    GCAAAAAGAG ACCACGCTCT GATCAATGCT ACTAGGACTG CACATATTAA AACAGAGCGT
    GAATCTCTAA AAAACATCAC GAAACCAGTG CCTGCAAAGA AAGCCAAAGA GGCTCCAAAG
    GTTAAGATTG CGCTGGAGCC TAAAAAGAAA GGAATCATCC CTGAGAAGGT CAAAGAAGTA
    AAGGAAACTT TACAGAAAGA ACCTGAGTTG GTCAAAGATA AAGTAGTTGA GCCAATAGAG
    AAGGAAATTG TTGTTGAGAA GGAAGTCAAA GGAGAAGAGC CTGAAATCAC CAAAGATATT
    CCAGAGGTGG AGGAAGAGGA CATTCCCTAC TTCCAGTGTT TCTTTGTGGA TGAGGATGAC
    ACTCAGTATC CGTTCTTCCC TTTCTCTCCA CCGCTTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149712
    Clone ID Related Accession (Same CDS sequence) XM_021467376.1
    Accession Version XM_021467376.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X45
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323051.1
    CDS338..8068
    Translation

    Target ORF information:

    RefSeq Version XM_021467376.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X45, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGTTGAAG TCATCAAAGA GAAAGCTAAA
    CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC AAGGAAAAGG TTCAACCAGC TCCTATAAAG
    AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG CCTGAACCAA CACCAGAACC TGAGAGAGTT
    GAAGCTCCAA AAGAAAAGGC AAAAGTAATC CCTAGGGAAA AAGTAGCTGA GATTACTAAA
    GAAAAACCAA AGCCAGCACC AAAGAAAGAG GCTGAAGTAA AGGAGAAAGC TACACCTGCT
    CCTGTGCACA AAGAGGTTGA AGTGTTCAAG GAGAAAGTTA AACAAGCTCC TGTAAAGAAA
    GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT GAACCAAAAC CTAAAAAGAC TGAAGTCATC
    AAAGCGAAAG TAAAACCAGC CCCTGAGAAA AAAGAGGCTG AAGTGAAGGA GAAAATTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATAAAGGAGA GAATTAAACC TGCTCCTGTA
    AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG GCTCCTGAAC CAAAACCCAA AAAAGAACCT
    GAAGTTATCA AAGAGAAAGT GAAACCAGCG CCAGAGAAGA AAGCACCCGA AATAACCAAA
    GAAAAACCAA AGCCAGCAGC AAAGAAAGTA GCTGAAGTCA TCAAAGACAG AACTAAACCA
    GTCCCTGAGA AGAAAGAGGC TGAAGTCATC AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG
    AAAGAGGCTA AAGTCATTGA AAAGAAAGTT ATACCAGCTC CCGTAAAGAA AGAGCCTGAA
    GTTGTTAAGG AAAAGGCTCC TGAACTGACA ACAAAAAAAG CACCTGAGAT AACCAAAGAA
    AAACCGAAGC CAGTACCAAA GAAAGAGGCT GAAGTCATTA AAGAGAAAGC TAAACTAGCT
    CCTGAAAAGA AAGAGGTTGA AGTCATCAAG GAGAAAGTTA AACCAGCTCC GTTAAAGAAA
    GAGCCTGAGG TTCATAAGGA AAAAGCACCT GAACCAAAAT CAGAACCTAC AAAAAAAGTT
    GAGGTTCCAA AGGAAAAGGC AAAACCAGTT GCTAAAGAAA AGGAACCTGA AATAACCAAA
    GAAAAGCCAA AACCAGCACC AAAGAAAGAA GCTGTAGTCA TCAAAGAAAA AGCTGTACCA
    GCCATCCTGA AGGAAGTGGC TGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149713
    Clone ID Related Accession (Same CDS sequence) XM_021467377.1
    Accession Version XM_021467377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X46
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA GGCTGAACCT
    GTAAAGGAGA TAACTAAACC AGCCCCTGAG AAGAAAGAGG CTGAAATAAT CAAGGAGAAA
    GTTAAAGCAG CACCTCTGAA GAAAGAAGTC AAGGAGAAAG CAAAACCAGC TCCTGTAAAG
    AAAGAAGCTG AAGTCATCAA ACAAAAAGCC AAACCAGCTC CTGAGAAGAA AGAGGCCAAA
    GTCATCAAGG AGAAAGTTAA ACCGGCTCCT GTAAAGATAG AGGCTAAACC TGTAAAGGAG
    ATAACTAAAC CAGCCCCTGA GAAGAAAGAG GCTGAAATAA TCAAGGAGAA GGTCAAATCA
    GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG GAGGAAGCAA AACCAGCTCC TGAAAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAAGTTAAA CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA
    ATAAAGGAAA AAGCTGAAGC AGTTTATGAG AAAAAAGAGG CTGAAGTAGC CAAGGAGATA
    GTTAAACCAA CTCCTTTAAA GAAGGAGCCC GAAGTTGTGA AGGAAAAGGC TCCTGAATCA
    AAACCCCAAA AAGAGGCTGA AATTATCAAA GAGAAAGTTA AACCTGCACC TGTAAAGAAA
    GAGCCTGAAG TTGCAAAAGT ACCAAAACCC AAAAAAGAGG CTGAACTCGC CAAAGAGAAA
    GTTAAACCTG CACCTGTAAA GAAAGAAGAA AAACCTAAGG CAGCACCAAA GAAAGAGGCT
    GAAGCTATTA AGGAGAAACG TACACCAGCT CCTGAAAAGA AAGTGGTAGA AGTCATCAAG
    GAGAAAGTCA AACCAGCCCC TGAGAAAAAA GCGGTTCTCA AGGAGAAAGT TAGACCTGCT
    CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA GAAAAGGCTC CTGAACCAAA ACCCCAAAAA
    GAGGTTGAAG TCATCAAAGA GAAAGCTAAA CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC
    AAGGAAAAGG TTCAACCAGC TCCTATAAAG AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG
    CCTGAACCAA CACCAGAACC TGAGAGAGTT GAAGCTCCAA AAGAAAAGGC AAAAGTAATC
    CCTAGGGAAA AAGTAGCTGA GATTACTAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGTAA AGGAGAAAGC TACACCTGCT CCTGTGCACA AAGAGGTTGA AGTGTTCAAG
    GAGAAAGTTA AACAAGCTCC TGTAAAGAAA GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT
    GAACCAAAAC CTAAAAAGAC TGAAGTCATC AAAGCGAAAG TAAAACCAGC CCCTGAGAAA
    AAAGAGGCTG AAGTGAAGGA GAAAATTAAA CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC
    ATAAAGGAGA GAATTAAACC TGCTCCTGTA AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG
    GCTCCTGAAC CAAAACCCAA AAAAGAACCT GAAGTTATCA AAGAGAAAGT GAAACCAGCG
    CCAGAGAAGA AAGCACCCGA AATAACCAAA GAAAAACCAA AGCCAGCAGC AAAGAAAGTA
    GCTGAAGTCA TCAAAGACAG AACTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG AAAGAGGCTA AAGTCATTGA AAAGAAAGTT
    ATACCAGCTC CCGTAAAGAA AGAGCCTGAA GTTGTTAAGG AAAAGGCTCC TGAACTGACA
    ACAAAAAAAG CACCTGAGAT AACCAAAGAA AAACCGAAGC CAGTACCAAA GAAAGAGGCT
    GAAGTCATTA AAGAGAAAGC TAAACTAGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAG
    GAGAAAGTTA AACCAGCTCC GTTAAAGAAA GAGCCTGAGG TTCATAAGGA AAAAGCACCT
    GAACCAAAAT CAGAACCTAC AAAAAAAGTT GAGGTTCCAA AGGAAAAGGC AAAACCAGTT
    GCTAAAGAAA AGGAACCTGA AATAACCAAA GAAAAGCCAA AACCAGCACC AAAGAAAGAA
    GCTGTAGTCA TCAAAGAAAA AGCTGTACCA GCCATCCTGA AGGAAGTGGC TGAAGTAAAG
    AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG GCTGAAATTA AGGAGAAAGT TAAACCAGCT
    TCTATAATGA AAGAGCCTGA AGTTGTGAAA CAAAAGACTC CTGAACCGAA ACCCAAAAAA
    GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT GAACCTAAAC CCAAAAAAGA GCCTGAAGTT
    GCTAAAGAGA AAGCCAAACC AGCACCTCAG AAGAAAGAAG TCATCAAAGA AAAAGTCAAA
    CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT GTCGAAGAAA AGGCACTTAA ACCAGAACCC
    AAAAAAGATG AAGTTGGTCT TATTGGTCTG GACCTGGAGC TCCTAAACTC CATCAACCAA
    AAACTGTCTC TCCTGGACTT GCTAAGGAAG GACATTGGAG AAATGAAAAG CGATCTGGAG
    TCCACTCAGA ATCAAATCAC ACTTTTACGG ATGGACAACA GAACGATCAA AGAGCCAGAG
    ACTATTGGTG TGAAAGTAAA ACCAGCAGTC AAGGAAAAAG CAAAAAGAGA CCACGCTCTG
    ATCAATGCTA CTAGGACTGC ACATATTAAA ACAGAGCGTG AATCTCTAAA AAACATCACG
    AAACCAGTGC CTGCAAAGAA AGCCAAAGAG GCTCCAAAGG TTAAGATTGC GCTGGAGCCT
    AAAAAGAAAG GAATCATCCC TGAGAAGGTC AAAGAAGTAA AGGAAACTTT ACAGAAAGAA
    CCTGAGTTGG TCAAAGATAA AGTAGTTGAG CCAATAGAGA AGGAAATTGT TGTTGAGAAG
    GAAGTCAAAG GAGAAGAGCC TGAAATCACC AAAGATATTC CAGAGGTGGA GGAAGAGGAC
    ATTCCCTACT TCCAGTGTTT CTTTGTGGAT GAGGATGACA CTCAGTATCC GTTCTTCCCT
    TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323052.1
    CDS338..8038
    Translation

    Target ORF information:

    RefSeq Version XM_021467377.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X46, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA GGCTGAACCT
    GTAAAGGAGA TAACTAAACC AGCCCCTGAG AAGAAAGAGG CTGAAATAAT CAAGGAGAAA
    GTTAAAGCAG CACCTCTGAA GAAAGAAGTC AAGGAGAAAG CAAAACCAGC TCCTGTAAAG
    AAAGAAGCTG AAGTCATCAA ACAAAAAGCC AAACCAGCTC CTGAGAAGAA AGAGGCCAAA
    GTCATCAAGG AGAAAGTTAA ACCGGCTCCT GTAAAGATAG AGGCTAAACC TGTAAAGGAG
    ATAACTAAAC CAGCCCCTGA GAAGAAAGAG GCTGAAATAA TCAAGGAGAA GGTCAAATCA
    GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG GAGGAAGCAA AACCAGCTCC TGAAAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAAGTTAAA CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA
    ATAAAGGAAA AAGCTGAAGC AGTTTATGAG AAAAAAGAGG CTGAAGTAGC CAAGGAGATA
    GTTAAACCAA CTCCTTTAAA GAAGGAGCCC GAAGTTGTGA AGGAAAAGGC TCCTGAATCA
    AAACCCCAAA AAGAGGCTGA AATTATCAAA GAGAAAGTTA AACCTGCACC TGTAAAGAAA
    GAGCCTGAAG TTGCAAAAGT ACCAAAACCC AAAAAAGAGG CTGAACTCGC CAAAGAGAAA
    GTTAAACCTG CACCTGTAAA GAAAGAAGAA AAACCTAAGG CAGCACCAAA GAAAGAGGCT
    GAAGCTATTA AGGAGAAACG TACACCAGCT CCTGAAAAGA AAGTGGTAGA AGTCATCAAG
    GAGAAAGTCA AACCAGCCCC TGAGAAAAAA GCGGTTCTCA AGGAGAAAGT TAGACCTGCT
    CCTGAAAAGA AAGAGCCTGA AGTTGCTAAA GAAAAGGCTC CTGAACCAAA ACCCCAAAAA
    GAGGTTGAAG TCATCAAAGA GAAAGCTAAA CCAGCCCTTA AGAAGAAAGA GCCTAAAGTC
    AAGGAAAAGG TTCAACCAGC TCCTATAAAG AAAGAGCCAG AGCTTCCCAA AGAAAAGGTG
    CCTGAACCAA CACCAGAACC TGAGAGAGTT GAAGCTCCAA AAGAAAAGGC AAAAGTAATC
    CCTAGGGAAA AAGTAGCTGA GATTACTAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGTAA AGGAGAAAGC TACACCTGCT CCTGTGCACA AAGAGGTTGA AGTGTTCAAG
    GAGAAAGTTA AACAAGCTCC TGTAAAGAAA GAGCCAGAAG TTCCTAAAGA AAAGGCTCCT
    GAACCAAAAC CTAAAAAGAC TGAAGTCATC AAAGCGAAAG TAAAACCAGC CCCTGAGAAA
    AAAGAGGCTG AAGTGAAGGA GAAAATTAAA CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC
    ATAAAGGAGA GAATTAAACC TGCTCCTGTA AAGAAAGAGC CTGAAGTTCC TAAAGAAAAG
    GCTCCTGAAC CAAAACCCAA AAAAGAACCT GAAGTTATCA AAGAGAAAGT GAAACCAGCG
    CCAGAGAAGA AAGCACCCGA AATAACCAAA GAAAAACCAA AGCCAGCAGC AAAGAAAGTA
    GCTGAAGTCA TCAAAGACAG AACTAAACCA GTCCCTGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG AAAGAGGCTA AAGTCATTGA AAAGAAAGTT
    ATACCAGCTC CCGTAAAGAA AGAGCCTGAA GTTGTTAAGG AAAAGGCTCC TGAACTGACA
    ACAAAAAAAG CACCTGAGAT AACCAAAGAA AAACCGAAGC CAGTACCAAA GAAAGAGGCT
    GAAGTCATTA AAGAGAAAGC TAAACTAGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAG
    GAGAAAGTTA AACCAGCTCC GTTAAAGAAA GAGCCTGAGG TTCATAAGGA AAAAGCACCT
    GAACCAAAAT CAGAACCTAC AAAAAAAGTT GAGGTTCCAA AGGAAAAGGC AAAACCAGTT
    GCTAAAGAAA AGGAACCTGA AATAACCAAA GAAAAGCCAA AACCAGCACC AAAGAAAGAA
    GCTGTAGTCA TCAAAGAAAA AGCTGTACCA GCCATCCTGA AGGAAGTGGC TGAAGTAAAG
    AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG GCTGAAATTA AGGAGAAAGT TAAACCAGCT
    TCTATAATGA AAGAGCCTGA AGTTGTGAAA CAAAAGACTC CTGAACCGAA ACCCAAAAAA
    GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT GAACCTAAAC CCAAAAAAGA GCCTGAAGTT
    GCTAAAGAGA AAGCCAAACC AGCACCTCAG AAGAAAGAAG TCATCAAAGA AAAAGTCAAA
    CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT GTCGAAGAAA AGGCACTTAA ACCAGAACCC
    AAAAAAGATG AAGTTGGTCT TATTGGTCTG GACCTGGAGC TCCTAAACTC CATCAACCAA
    AAACTGTCTC TCCTGGACTT GCTAAGGAAG GACATTGGAG AAATGAAAAG CGATCTGGAG
    TCCACTCAGA ATCAAATCAC ACTTTTACGG ATGGACAACA GAACGATCAA AGAGCCAGAG
    ACTATTGGTG TGAAAGTAAA ACCAGCAGTC AAGGAAAAAG CAAAAAGAGA CCACGCTCTG
    ATCAATGCTA CTAGGACTGC ACATATTAAA ACAGAGCGTG AATCTCTAAA AAACATCACG
    AAACCAGTGC CTGCAAAGAA AGCCAAAGAG GCTCCAAAGG TTAAGATTGC GCTGGAGCCT
    AAAAAGAAAG GAATCATCCC TGAGAAGGTC AAAGAAGTAA AGGAAACTTT ACAGAAAGAA
    CCTGAGTTGG TCAAAGATAA AGTAGTTGAG CCAATAGAGA AGGAAATTGT TGTTGAGAAG
    GAAGTCAAAG GAGAAGAGCC TGAAATCACC AAAGATATTC CAGAGGTGGA GGAAGAGGAC
    ATTCCCTACT TCCAGTGTTT CTTTGTGGAT GAGGATGACA CTCAGTATCC GTTCTTCCCT
    TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149714
    Clone ID Related Accession (Same CDS sequence) XM_021467378.1
    Accession Version XM_021467378.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7659bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X47
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGAAGTC
    ATCAAAGAAA AAGTCAAACT TGCTCCTGTA AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG
    GCACTTAAAC CAGAACCCAA AAAAGATGAA GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC
    CTAAACTCCA TCAACCAAAA ACTGTCTCTC CTGGACTTGC TAAGGAAGGA CATTGGAGAA
    ATGAAAAGCG ATCTGGAGTC CACTCAGAAT CAAATCACAC TTTTACGGAT GGACAACAGA
    ACGATCAAAG AGCCAGAGAC TATTGGTGTG AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA
    AAAAGAGACC ACGCTCTGAT CAATGCTACT AGGACTGCAC ATATTAAAAC AGAGCGTGAA
    TCTCTAAAAA ACATCACGAA ACCAGTGCCT GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT
    AAGATTGCGC TGGAGCCTAA AAAGAAAGGA ATCATCCCTG AGAAGGTCAA AGAAGTAAAG
    GAAACTTTAC AGAAAGAACC TGAGTTGGTC AAAGATAAAG TAGTTGAGCC AATAGAGAAG
    GAAATTGTTG TTGAGAAGGA AGTCAAAGGA GAAGAGCCTG AAATCACCAA AGATATTCCA
    GAGGTGGAGG AAGAGGACAT TCCCTACTTC CAGTGTTTCT TTGTGGATGA GGATGACACT
    CAGTATCCGT TCTTCCCTTT CTCTCCACCG CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323053.1
    CDS338..7996
    Translation

    Target ORF information:

    RefSeq Version XM_021467378.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X47, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467378.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGC GGTTCTCAAG
    GAGAAAGTTA GACCTGCTCC TGAAAAGAAA GAGCCTGAAG TTGCTAAAGA AAAGGCTCCT
    GAACCAAAAC CCCAAAAAGA GGTTGAAGTC ATCAAAGAGA AAGCTAAACC AGCCCTTAAG
    AAGAAAGAGC CTAAAGTCAA GGAAAAGGTT CAACCAGCTC CTATAAAGAA AGAGCCAGAG
    CTTCCCAAAG AAAAGGTGCC TGAACCAACA CCAGAACCTG AGAGAGTTGA AGCTCCAAAA
    GAAAAGGCAA AAGTAATCCC TAGGGAAAAA GTAGCTGAGA TTACTAAAGA AAAACCAAAG
    CCAGCACCAA AGAAAGAGGC TGAAGTAAAG GAGAAAGCTA CACCTGCTCC TGTGCACAAA
    GAGGTTGAAG TGTTCAAGGA GAAAGTTAAA CAAGCTCCTG TAAAGAAAGA GCCAGAAGTT
    CCTAAAGAAA AGGCTCCTGA ACCAAAACCT AAAAAGACTG AAGTCATCAA AGCGAAAGTA
    AAACCAGCCC CTGAGAAAAA AGAGGCTGAA GTGAAGGAGA AAATTAAACC AGTCCCTGAG
    AAGAAAGAGG CTGAAGTCAT AAAGGAGAGA ATTAAACCTG CTCCTGTAAA GAAAGAGCCT
    GAAGTTCCTA AAGAAAAGGC TCCTGAACCA AAACCCAAAA AAGAACCTGA AGTTATCAAA
    GAGAAAGTGA AACCAGCGCC AGAGAAGAAA GCACCCGAAA TAACCAAAGA AAAACCAAAG
    CCAGCAGCAA AGAAAGTAGC TGAAGTCATC AAAGACAGAA CTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATCAA GGAGAAACCA AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA
    GTCATTGAAA AGAAAGTTAT ACCAGCTCCC GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA
    AAGGCTCCTG AACTGACAAC AAAAAAAGCA CCTGAGATAA CCAAAGAAAA ACCGAAGCCA
    GTACCAAAGA AAGAGGCTGA AGTCATTAAA GAGAAAGCTA AACTAGCTCC TGAAAAGAAA
    GAGGTTGAAG TCATCAAGGA GAAAGTTAAA CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT
    CATAAGGAAA AAGCACCTGA ACCAAAATCA GAACCTACAA AAAAAGTTGA GGTTCCAAAG
    GAAAAGGCAA AACCAGTTGC TAAAGAAAAG GAACCTGAAA TAACCAAAGA AAAGCCAAAA
    CCAGCACCAA AGAAAGAAGC TGTAGTCATC AAAGAAAAAG CTGTACCAGC CATCCTGAAG
    GAAGTGGCTG AAGTAAAGAA AGTTAAAGCA GCTCCTGAGA AGAAAGTGGC TGAAATTAAG
    GAGAAAGTTA AACCAGCTTC TATAATGAAA GAGCCTGAAG TTGTGAAACA AAAGACTCCT
    GAACCGAAAC CCAAAAAAGA GCCTGAAGTT GTGAAAGAAA AGGCTCCTGA ACCTAAACCC
    AAAAAAGAGC CTGAAGTTGC TAAAGAGAAA GCCAAACCAG CACCTCAGAA GAAAGAAGTC
    ATCAAAGAAA AAGTCAAACT TGCTCCTGTA AAGAAAGTGC CCGAAGTTGT CGAAGAAAAG
    GCACTTAAAC CAGAACCCAA AAAAGATGAA GTTGGTCTTA TTGGTCTGGA CCTGGAGCTC
    CTAAACTCCA TCAACCAAAA ACTGTCTCTC CTGGACTTGC TAAGGAAGGA CATTGGAGAA
    ATGAAAAGCG ATCTGGAGTC CACTCAGAAT CAAATCACAC TTTTACGGAT GGACAACAGA
    ACGATCAAAG AGCCAGAGAC TATTGGTGTG AAAGTAAAAC CAGCAGTCAA GGAAAAAGCA
    AAAAGAGACC ACGCTCTGAT CAATGCTACT AGGACTGCAC ATATTAAAAC AGAGCGTGAA
    TCTCTAAAAA ACATCACGAA ACCAGTGCCT GCAAAGAAAG CCAAAGAGGC TCCAAAGGTT
    AAGATTGCGC TGGAGCCTAA AAAGAAAGGA ATCATCCCTG AGAAGGTCAA AGAAGTAAAG
    GAAACTTTAC AGAAAGAACC TGAGTTGGTC AAAGATAAAG TAGTTGAGCC AATAGAGAAG
    GAAATTGTTG TTGAGAAGGA AGTCAAAGGA GAAGAGCCTG AAATCACCAA AGATATTCCA
    GAGGTGGAGG AAGAGGACAT TCCCTACTTC CAGTGTTTCT TTGTGGATGA GGATGACACT
    CAGTATCCGT TCTTCCCTTT CTCTCCACCG CTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149715
    Clone ID Related Accession (Same CDS sequence) XM_021467379.1
    Accession Version XM_021467379.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X48
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGTAAAGGAG AAAGCTACAC CTGCTCCTGT GCACAAAGAG
    GTTGAAGTGT TCAAGGAGAA AGTTAAACAA GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCTAAA AAGACTGAAG TCATCAAAGC GAAAGTAAAA
    CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG AAGGAGAAAA TTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATAAA GGAGAGAATT AAACCTGCTC CTGTAAAGAA AGAGCCTGAA
    GTTCCTAAAG AAAAGGCTCC TGAACCAAAA CCCAAAAAAG AACCTGAAGT TATCAAAGAG
    AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA CCCGAAATAA CCAAAGAAAA ACCAAAGCCA
    GCAGCAAAGA AAGTAGCTGA AGTCATCAAA GACAGAACTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAACCAAAA GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC
    ATTGAAAAGA AAGTTATACC AGCTCCCGTA AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG
    GCTCCTGAAC TGACAACAAA AAAAGCACCT GAGATAACCA AAGAAAAACC GAAGCCAGTA
    CCAAAGAAAG AGGCTGAAGT CATTAAAGAG AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG
    GTTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT
    AAGGAAAAAG CACCTGAACC AAAATCAGAA CCTACAAAAA AAGTTGAGGT TCCAAAGGAA
    AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA CCTGAAATAA CCAAAGAAAA GCCAAAACCA
    GCACCAAAGA AAGAAGCTGT AGTCATCAAA GAAAAAGCTG TACCAGCCAT CCTGAAGGAA
    GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT CCTGAGAAGA AAGTGGCTGA AATTAAGGAG
    AAAGTTAAAC CAGCTTCTAT AATGAAAGAG CCTGAAGTTG TGAAACAAAA GACTCCTGAA
    CCGAAACCCA AAAAAGAGCC TGAAGTTGTG AAAGAAAAGG CTCCTGAACC TAAACCCAAA
    AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC AAACCAGCAC CTCAGAAGAA AGAAGTCATC
    AAAGAAAAAG TCAAACTTGC TCCTGTAAAG AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA
    CTTAAACCAG AACCCAAAAA AGATGAAGTT GGTCTTATTG GTCTGGACCT GGAGCTCCTA
    AACTCCATCA ACCAAAAACT GTCTCTCCTG GACTTGCTAA GGAAGGACAT TGGAGAAATG
    AAAAGCGATC TGGAGTCCAC TCAGAATCAA ATCACACTTT TACGGATGGA CAACAGAACG
    ATCAAAGAGC CAGAGACTAT TGGTGTGAAA GTAAAACCAG CAGTCAAGGA AAAAGCAAAA
    AGAGACCACG CTCTGATCAA TGCTACTAGG ACTGCACATA TTAAAACAGA GCGTGAATCT
    CTAAAAAACA TCACGAAACC AGTGCCTGCA AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG
    ATTGCGCTGG AGCCTAAAAA GAAAGGAATC ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA
    ACTTTACAGA AAGAACCTGA GTTGGTCAAA GATAAAGTAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323054.1
    CDS338..7993
    Translation

    Target ORF information:

    RefSeq Version XM_021467379.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X48, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGTAAAGGAG AAAGCTACAC CTGCTCCTGT GCACAAAGAG
    GTTGAAGTGT TCAAGGAGAA AGTTAAACAA GCTCCTGTAA AGAAAGAGCC AGAAGTTCCT
    AAAGAAAAGG CTCCTGAACC AAAACCTAAA AAGACTGAAG TCATCAAAGC GAAAGTAAAA
    CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG AAGGAGAAAA TTAAACCAGT CCCTGAGAAG
    AAAGAGGCTG AAGTCATAAA GGAGAGAATT AAACCTGCTC CTGTAAAGAA AGAGCCTGAA
    GTTCCTAAAG AAAAGGCTCC TGAACCAAAA CCCAAAAAAG AACCTGAAGT TATCAAAGAG
    AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA CCCGAAATAA CCAAAGAAAA ACCAAAGCCA
    GCAGCAAAGA AAGTAGCTGA AGTCATCAAA GACAGAACTA AACCAGTCCC TGAGAAGAAA
    GAGGCTGAAG TCATCAAGGA GAAACCAAAA GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC
    ATTGAAAAGA AAGTTATACC AGCTCCCGTA AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG
    GCTCCTGAAC TGACAACAAA AAAAGCACCT GAGATAACCA AAGAAAAACC GAAGCCAGTA
    CCAAAGAAAG AGGCTGAAGT CATTAAAGAG AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG
    GTTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT
    AAGGAAAAAG CACCTGAACC AAAATCAGAA CCTACAAAAA AAGTTGAGGT TCCAAAGGAA
    AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA CCTGAAATAA CCAAAGAAAA GCCAAAACCA
    GCACCAAAGA AAGAAGCTGT AGTCATCAAA GAAAAAGCTG TACCAGCCAT CCTGAAGGAA
    GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT CCTGAGAAGA AAGTGGCTGA AATTAAGGAG
    AAAGTTAAAC CAGCTTCTAT AATGAAAGAG CCTGAAGTTG TGAAACAAAA GACTCCTGAA
    CCGAAACCCA AAAAAGAGCC TGAAGTTGTG AAAGAAAAGG CTCCTGAACC TAAACCCAAA
    AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC AAACCAGCAC CTCAGAAGAA AGAAGTCATC
    AAAGAAAAAG TCAAACTTGC TCCTGTAAAG AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA
    CTTAAACCAG AACCCAAAAA AGATGAAGTT GGTCTTATTG GTCTGGACCT GGAGCTCCTA
    AACTCCATCA ACCAAAAACT GTCTCTCCTG GACTTGCTAA GGAAGGACAT TGGAGAAATG
    AAAAGCGATC TGGAGTCCAC TCAGAATCAA ATCACACTTT TACGGATGGA CAACAGAACG
    ATCAAAGAGC CAGAGACTAT TGGTGTGAAA GTAAAACCAG CAGTCAAGGA AAAAGCAAAA
    AGAGACCACG CTCTGATCAA TGCTACTAGG ACTGCACATA TTAAAACAGA GCGTGAATCT
    CTAAAAAACA TCACGAAACC AGTGCCTGCA AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG
    ATTGCGCTGG AGCCTAAAAA GAAAGGAATC ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA
    ACTTTACAGA AAGAACCTGA GTTGGTCAAA GATAAAGTAG TTGAGCCAAT AGAGAAGGAA
    ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA GAGCCTGAAA TCACCAAAGA TATTCCAGAG
    GTGGAGGAAG AGGACATTCC CTACTTCCAG TGTTTCTTTG TGGATGAGGA TGACACTCAG
    TATCCGTTCT TCCCTTTCTC TCCACCGCTT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149716
    Clone ID Related Accession (Same CDS sequence) XM_021467380.1
    Accession Version XM_021467380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X49
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323055.1
    CDS338..7903
    Translation

    Target ORF information:

    RefSeq Version XM_021467380.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X49, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGAG
    GCTAAAATTG TGAAGGAGAA ACCCAAAGCA CATCCTGCAA AGAAAGAAAA AGCTAAAGAT
    ATTAAGGAAA AGGCAAAATC TGGTCAAAAA GAAAAAGTTG CTGAGGCTGT CAAGGAAAAA
    TCGAAGCCAG TAAAGAAAGA AGCTGAAAAA ATTGATGCCA AAGTCAAACC AGCAGCAGAA
    AAGAAAGTGG ATGAAGCCCC CAAAGAGAAA CCTAAAGTGA AGAAAGAGGC TGAAGTAATC
    AAAAAGATTA AACTAGAGCC TGAGAGGAAA GAAGCTAAAA CTCCCAAGGC CAAAATTGAA
    CCAGCTGGTT TGAAGAAAGA TGCTGAAGCT GCTAAGGAGA AAGCAAAACC TGCTCCAGTC
    AAGAAAGGGC CTGAGGTTTC CAGAGAAAAA GCCAAACCAG AGCCCGCAAA AAAAGAGCTT
    GAAGCGGTCA AAGAGAAAGC AAAACCAGCA CCAGCTAAAA AAGATGAAGT CAAAGAGAAA
    GAAAAACCAG CTCCTGTAAA GAAAGCTGAA GAAATTAAAA AGGCAAAAGC AGCTCCTTCA
    AGGAAAGAAG CTGTAAAGGA AAAACCCAAA CCTGTTTCCG AAAAGAAAGA ACCTGCTGTT
    GCAGTTGAAA AAGCCAAGCC AAAATCTACA AAGAAAGAAG CTGAAGTCGA GGAAAAAGTG
    AAACCTGCTC CTGAGAAGAA AGAAGCTGAA GTCATCATGG TGAAAACTAA ACCAGTTCTT
    AAAAAGAAAG AGGCTGAAGT AATCAAAGAG GTTAAACCAG CCCTTGAGAA GAAAGAGGCT
    GAAGTCATCA AGGAGAAAGT TAAACCAGCT CCTGTAAAGA AAGAGCCTGA AGTTGTTAAA
    GAAAAGGAAC CTGAACCAAA ACCCAAAAGA GAGCCAGAAA TTATTAAGGA GAAATCAGCA
    CCACCAAAGA AAGTTGAGGT TCCAAAACAA AAGGCTCCTG AAAAGAAAGC ACCTGAAATA
    ACCAAAGAAA AACCAAAGCC AGCACCAAAG AAAGAGGCTG AAGCTGTAAA AGAGAAAGCT
    AAACCAGAGA AGAAAGAGGC TGAAGCCGTA AAGGAGAAAA CGAAACCAGC CCCTGAGAAA
    GAGACTGAAA TAATCAAGGA TAAAATTAAA GCAGCTCCTG TGAAGAAAGA AGTTGAAGTC
    AAGAAGGAAG CAAAACCAGC TCCTGTAAAG AAAGAAACTG AAGTCATCAA AGAAAAAGAA
    AAACCAGCTC CTGAGAAGAA AGAGGCTGAA GCTGTAAAGG AGAAAACGAA ACCAACCTCT
    GAGAAGAAAG AGGCTGAAAT AATTAAGGAG AAAGTTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGTAAAAA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AAACAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA TAGTTAAACA AGCTCCTGTG
    AAGAAAGAGG CTGAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAAGT TAAAGCAGCA CCTCTGAAGA AAGAAGTCAA GGAGAAAGCA
    AAACCAGCTC CTGTAAAGAA AGAAGCTGAA GTCATCAAAC AAAAAGCCAA ACCAGCTCCT
    GAGAAGAAAG AGGCCAAAGT CATCAAGGAG AAAGTTAAAC CGGCTCCTGT AAAGATAGAG
    GCTAAACCTG TAAAGGAGAT AACTAAACCA GCCCCTGAGA AGAAAGAGGC TGAAATAATC
    AAGGAGAAGG TCAAATCAGC TCCTGTGAAG AAAGAAGTTG AAGTCAAGGA GGAAGCAAAA
    CCAGCTCCTG AAAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC TGCTCCTGTG
    AGGAAAGAAG CTGAAGAAAT AAAGGAAAAA GCTGAAGCAG TTTATGAGAA AAAAGAGGCT
    GAAGTAGCCA AGGAGATAGT TAAACCAACT CCTTTAAAGA AGGAGCCCGA AGTTGTGAAG
    GAAAAGGCTC CTGAATCAAA ACCCCAAAAA GAGGCTGAAA TTATCAAAGA GAAAGTTAAA
    CCTGCACCTG TAAAGAAAGA GCCTGAAGTT GCAAAAGTAC CAAAACCCAA AAAAGAGGCT
    GAACTCGCCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAAGAAAA ACCTAAGGCA
    GCACCAAAGA AAGAGGCTGA AGCTATTAAG GAGAAACGTA CACCAGCTCC TGAAAAGAAA
    GTGGTAGAAG TCATCAAGGA GAAAGTCAAA CCAGCCCCTG AGAAAAAAGA GGCTGAAGTG
    AAGGAGAAAA TTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATAAA GGAGAGAATT
    AAACCTGCTC CTGTAAAGAA AGAGCCTGAA GTTCCTAAAG AAAAGGCTCC TGAACCAAAA
    CCCAAAAAAG AACCTGAAGT TATCAAAGAG AAAGTGAAAC CAGCGCCAGA GAAGAAAGCA
    CCCGAAATAA CCAAAGAAAA ACCAAAGCCA GCAGCAAAGA AAGTAGCTGA AGTCATCAAA
    GACAGAACTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GAAACCAAAA
    GCAGTTCCCG AGAAGAAAGA GGCTAAAGTC ATTGAAAAGA AAGTTATACC AGCTCCCGTA
    AAGAAAGAGC CTGAAGTTGT TAAGGAAAAG GCTCCTGAAC TGACAACAAA AAAAGCACCT
    GAGATAACCA AAGAAAAACC GAAGCCAGTA CCAAAGAAAG AGGCTGAAGT CATTAAAGAG
    AAAGCTAAAC TAGCTCCTGA AAAGAAAGAG GTTGAAGTCA TCAAGGAGAA AGTTAAACCA
    GCTCCGTTAA AGAAAGAGCC TGAGGTTCAT AAGGAAAAAG CACCTGAACC AAAATCAGAA
    CCTACAAAAA AAGTTGAGGT TCCAAAGGAA AAGGCAAAAC CAGTTGCTAA AGAAAAGGAA
    CCTGAAATAA CCAAAGAAAA GCCAAAACCA GCACCAAAGA AAGAAGCTGT AGTCATCAAA
    GAAAAAGCTG TACCAGCCAT CCTGAAGGAA GTGGCTGAAG TAAAGAAAGT TAAAGCAGCT
    CCTGAGAAGA AAGTGGCTGA AATTAAGGAG AAAGTTAAAC CAGCTTCTAT AATGAAAGAG
    CCTGAAGTTG TGAAACAAAA GACTCCTGAA CCGAAACCCA AAAAAGAGCC TGAAGTTGTG
    AAAGAAAAGG CTCCTGAACC TAAACCCAAA AAAGAGCCTG AAGTTGCTAA AGAGAAAGCC
    AAACCAGCAC CTCAGAAGAA AGAAGTCATC AAAGAAAAAG TCAAACTTGC TCCTGTAAAG
    AAAGTGCCCG AAGTTGTCGA AGAAAAGGCA CTTAAACCAG AACCCAAAAA AGATGAAGTT
    GGTCTTATTG GTCTGGACCT GGAGCTCCTA AACTCCATCA ACCAAAAACT GTCTCTCCTG
    GACTTGCTAA GGAAGGACAT TGGAGAAATG AAAAGCGATC TGGAGTCCAC TCAGAATCAA
    ATCACACTTT TACGGATGGA CAACAGAACG ATCAAAGAGC CAGAGACTAT TGGTGTGAAA
    GTAAAACCAG CAGTCAAGGA AAAAGCAAAA AGAGACCACG CTCTGATCAA TGCTACTAGG
    ACTGCACATA TTAAAACAGA GCGTGAATCT CTAAAAAACA TCACGAAACC AGTGCCTGCA
    AAGAAAGCCA AAGAGGCTCC AAAGGTTAAG ATTGCGCTGG AGCCTAAAAA GAAAGGAATC
    ATCCCTGAGA AGGTCAAAGA AGTAAAGGAA ACTTTACAGA AAGAACCTGA GTTGGTCAAA
    GATAAAGTAG TTGAGCCAAT AGAGAAGGAA ATTGTTGTTG AGAAGGAAGT CAAAGGAGAA
    GAGCCTGAAA TCACCAAAGA TATTCCAGAG GTGGAGGAAG AGGACATTCC CTACTTCCAG
    TGTTTCTTTG TGGATGAGGA TGACACTCAG TATCCGTTCT TCCCTTTCTC TCCACCGCTT
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149717
    Clone ID Related Accession (Same CDS sequence) XM_021467381.1
    Accession Version XM_021467381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product titin isoform X50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTCT TGAGCAAGTG AAAGAAGCCA AAGAGAAAAA AGCTAGAGAG
    GAGATATACA AAATCATTCA AGAAATGAGG GAGGAGAAGG CTGAAGGTGT GGAGGAAGAG
    ATCGTGTTAC AACAGATAAA CATAACAGTA GAAAAGGTCA AGAAAGAAGA AACTGTCAAA
    AAGGAGGAAA AGGCTAAAAG AAAAGAAAAG GCTCATAAAC CAAAGGATGC AAAGATTGCC
    AAAGACAAAC CTAAAGCAGT CCCAGTAAAG CAAGAGGATG AAGTAGCCAA GAAAGAAACC
    AAACCAGCAC CATCTAAGAA AGAGGCTAAA ATTGTGAAGG AGAAACCCAA AGCACATCCT
    GCAAAGAAAG AAAAAGCTAA AGATATTAAG GAAAAGGCAA AATCTGGTCA AAAAGAAAAA
    GTTGCTGAGG CTGTCAAGGA AAAATCGAAG CCAGTAAAGA AAGAAGCTGA AAAAATTGAT
    GCCAAAGTCA AACCAGCAGC AGAAAAGAAA GTGGATGAAG CCCCCAAAGA GAAACCTAAA
    GTGAAGAAAG AGGCTGAAGT AATCAAAAAG ATTAAACTAG AGCCTGAGAG GAAAGAAGCT
    AAAACTCCCA AGGCCAAAAT TGAACCAGCT GGTTTGAAGA AAGATGCTGA AGCTGCTAAG
    GAGAAAGCAA AACCTGCTCC AGTCAAGAAA GGGCCTGAGG TTTCCAGAGA AAAAGCCAAA
    CCAGAGCCCG CAAAAAAAGA GCTTGAAGCG GTCAAAGAGA AAGCAAAACC AGCACCAGCT
    AAAAAAGATG AAGTCAAAGA GAAAGAAAAA CCAGCTCCTG TAAAGAAAGC TGAAGAAATT
    AAAAAGGCAA AAGCAGCTCC TTCAAGGAAA GAAGCTGTAA AGGAAAAACC CAAACCTGTT
    TCCGAAAAGA AAGAACCTGC TGTTGCAGTT GAAAAAGCCA AGCCAAAATC TACAAAGAAA
    GAAGCTGAAG TCGAGGAAAA AGTGAAACCT GCTCCTGAGA AGAAAGAAGC TGAAGTCATC
    ATGGTGAAAA CTAAACCAGT TCTTAAAAAG AAAGAGGCTG AAGTAATCAA AGAGGTTAAA
    CCAGCCCTTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC AGCTCCTGTA
    AAGAAAGAGC CTGAAGTTGT TAAAGAAAAG GAACCTGAAC CAAAACCCAA AAGAGAGCCA
    GAAATTATTA AGGAGAAATC AGCACCACCA AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT
    CCTGAAAAGA AAGCACCTGA AATAACCAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGCTG TAAAAGAGAA AGCTAAACCA GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG
    AAAACGAAAC CAGCCCCTGA GAAAGAGACT GAAATAATCA AGGATAAAAT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAACCA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CTCTGAGAAG AAAGAGGCTG AAATAATTAA GGAGAAAGTT
    AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA GTCAAGGAGG AAGCAAAACC AGCTCCTGTA
    AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA GAAAAAACAG CTCCTGAGAA GAAAGAGGCT
    GAAGCTGTAA AGGAGAAAAC GAAACCAACC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGATAGTTA AACAAGCTCC TGTGAAGAAA GAGGCTGAAC CTGTAAAGGA GATAACTAAA
    CCAGCCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323056.1
    CDS337..6759
    Translation

    Target ORF information:

    RefSeq Version XM_021467381.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X50, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTCT TGAGCAAGTG AAAGAAGCCA AAGAGAAAAA AGCTAGAGAG
    GAGATATACA AAATCATTCA AGAAATGAGG GAGGAGAAGG CTGAAGGTGT GGAGGAAGAG
    ATCGTGTTAC AACAGATAAA CATAACAGTA GAAAAGGTCA AGAAAGAAGA AACTGTCAAA
    AAGGAGGAAA AGGCTAAAAG AAAAGAAAAG GCTCATAAAC CAAAGGATGC AAAGATTGCC
    AAAGACAAAC CTAAAGCAGT CCCAGTAAAG CAAGAGGATG AAGTAGCCAA GAAAGAAACC
    AAACCAGCAC CATCTAAGAA AGAGGCTAAA ATTGTGAAGG AGAAACCCAA AGCACATCCT
    GCAAAGAAAG AAAAAGCTAA AGATATTAAG GAAAAGGCAA AATCTGGTCA AAAAGAAAAA
    GTTGCTGAGG CTGTCAAGGA AAAATCGAAG CCAGTAAAGA AAGAAGCTGA AAAAATTGAT
    GCCAAAGTCA AACCAGCAGC AGAAAAGAAA GTGGATGAAG CCCCCAAAGA GAAACCTAAA
    GTGAAGAAAG AGGCTGAAGT AATCAAAAAG ATTAAACTAG AGCCTGAGAG GAAAGAAGCT
    AAAACTCCCA AGGCCAAAAT TGAACCAGCT GGTTTGAAGA AAGATGCTGA AGCTGCTAAG
    GAGAAAGCAA AACCTGCTCC AGTCAAGAAA GGGCCTGAGG TTTCCAGAGA AAAAGCCAAA
    CCAGAGCCCG CAAAAAAAGA GCTTGAAGCG GTCAAAGAGA AAGCAAAACC AGCACCAGCT
    AAAAAAGATG AAGTCAAAGA GAAAGAAAAA CCAGCTCCTG TAAAGAAAGC TGAAGAAATT
    AAAAAGGCAA AAGCAGCTCC TTCAAGGAAA GAAGCTGTAA AGGAAAAACC CAAACCTGTT
    TCCGAAAAGA AAGAACCTGC TGTTGCAGTT GAAAAAGCCA AGCCAAAATC TACAAAGAAA
    GAAGCTGAAG TCGAGGAAAA AGTGAAACCT GCTCCTGAGA AGAAAGAAGC TGAAGTCATC
    ATGGTGAAAA CTAAACCAGT TCTTAAAAAG AAAGAGGCTG AAGTAATCAA AGAGGTTAAA
    CCAGCCCTTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AAGTTAAACC AGCTCCTGTA
    AAGAAAGAGC CTGAAGTTGT TAAAGAAAAG GAACCTGAAC CAAAACCCAA AAGAGAGCCA
    GAAATTATTA AGGAGAAATC AGCACCACCA AAGAAAGTTG AGGTTCCAAA ACAAAAGGCT
    CCTGAAAAGA AAGCACCTGA AATAACCAAA GAAAAACCAA AGCCAGCACC AAAGAAAGAG
    GCTGAAGCTG TAAAAGAGAA AGCTAAACCA GAGAAGAAAG AGGCTGAAGC CGTAAAGGAG
    AAAACGAAAC CAGCCCCTGA GAAAGAGACT GAAATAATCA AGGATAAAAT TAAAGCAGCT
    CCTGTGAAGA AAGAAGTTGA AGTCAAGAAG GAAGCAAAAC CAGCTCCTGT AAAGAAAGAA
    ACTGAAGTCA TCAAAGAAAA AGAAAAACCA GCTCCTGAGA AGAAAGAGGC TGAAGCTGTA
    AAGGAGAAAA CGAAACCAAC CTCTGAGAAG AAAGAGGCTG AAATAATTAA GGAGAAAGTT
    AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA GTCAAGGAGG AAGCAAAACC AGCTCCTGTA
    AAAAAAGAAA CTGAAGTCAT CAAAGAAAAA GAAAAAACAG CTCCTGAGAA GAAAGAGGCT
    GAAGCTGTAA AGGAGAAAAC GAAACCAACC CCTGAGAAGA AAGAGGCTGA AGTCATCAAG
    GAGATAGTTA AACAAGCTCC TGTGAAGAAA GAGGCTGAAC CTGTAAAGGA GATAACTAAA
    CCAGCCCCTG AGAAGAAAGA GGCTGAAATA ATCAAGGAGA AAGTTAAAGC AGCACCTCTG
    AAGAAAGAAG TCAAGGAGAA AGCAAAACCA GCTCCTGTAA AGAAAGAAGC TGAAGTCATC
    AAACAAAAAG CCAAACCAGC TCCTGAGAAG AAAGAGGCCA AAGTCATCAA GGAGAAAGTT
    AAACCGGCTC CTGTAAAGAT AGAGGCTAAA CCTGTAAAGG AGATAACTAA ACCAGCCCCT
    GAGAAGAAAG AGGCTGAAAT AATCAAGGAG AAGGTCAAAT CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGGAGGAAGC AAAACCAGCT CCTGAAAAGA AAGAGGCTGA AGTCATCAAG
    GAGAAAGTTA AACCTGCTCC TGTGAGGAAA GAAGCTGAAG AAATAAAGGA AAAAGCTGAA
    GCAGTTTATG AGAAAAAAGA GGCTGAAGTA GCCAAGGAGA TAGTTAAACC AACTCCTTTA
    AAGAAGGAGC CCGAAGTTGT GAAGGAAAAG GCTCCTGAAT CAAAACCCCA AAAAGAGGCT
    GAAATTATCA AAGAGAAAGT TAAACCTGCA CCTGTAAAGA AAGAGCCTGA AGTTGCAAAA
    GTACCAAAAC CCAAAAAAGA GGCTGAACTC GCCAAAGAGA AAGTTAAACC TGCACCTGTA
    AAGAAAGAAG AAAAACCTAA GGCAGCACCA AAGAAAGAGG CTGAAGCTAT TAAGGAGAAA
    CGTACACCAG CTCCTGAAAA GAAAGTGGTA GAAGTCATCA AGGAGAAAGT CAAACCAGCC
    CCTGAGAAAA AAGCGGTTCT CAAGGAGAAA GTTAGACCTG CTCCTGAAAA GAAAGAGCCT
    GAAGTTGCTA AAGAAAAGGC TCCTGAACCA AAACCCCAAA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTG GCTGAAGTAA AGAAAGTTAA AGCAGCTCCT
    GAGAAGAAAG TGGCTGAAAT TAAGGAGAAA GTTAAACCAG CTTCTATAAT GAAAGAGCCT
    GAAGTTGTGA AACAAAAGAC TCCTGAACCG AAACCCAAAA AAGAGCCTGA AGTTGTGAAA
    GAAAAGGCTC CTGAACCTAA ACCCAAAAAA GAGCCTGAAG TTGCTAAAGA GAAAGCCAAA
    CCAGCACCTC AGAAGAAAGA AGTCATCAAA GAAAAAGTCA AACTTGCTCC TGTAAAGAAA
    GTGCCCGAAG TTGTCGAAGA AAAGGCACTT AAACCAGAAC CCAAAAAAGA TGAAGTTGGT
    CTTATTGGTC TGGACCTGGA GCTCCTAAAC TCCATCAACC AAAAACTGTC TCTCCTGGAC
    TTGCTAAGGA AGGACATTGG AGAAATGAAA AGCGATCTGG AGTCCACTCA GAATCAAATC
    ACACTTTTAC GGATGGACAA CAGAACGATC AAAGAGCCAG AGACTATTGG TGTGAAAGTA
    AAACCAGCAG TCAAGGAAAA AGCAAAAAGA GACCACGCTC TGATCAATGC TACTAGGACT
    GCACATATTA AAACAGAGCG TGAATCTCTA AAAAACATCA CGAAACCAGT GCCTGCAAAG
    AAAGCCAAAG AGGCTCCAAA GGTTAAGATT GCGCTGGAGC CTAAAAAGAA AGGAATCATC
    CCTGAGAAGG TCAAAGAAGT AAAGGAAACT TTACAGAAAG AACCTGAGTT GGTCAAAGAT
    AAAGTAGTTG AGCCAATAGA GAAGGAAATT GTTGTTGAGA AGGAAGTCAA AGGAGAAGAG
    CCTGAAATCA CCAAAGATAT TCCAGAGGTG GAGGAAGAGG ACATTCCCTA CTTCCAGTGT
    TTCTTTGTGG ATGAGGATGA CACTCAGTAT CCGTTCTTCC CTTTCTCTCC ACCGCTTTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55696
    Clone ID Related Accession (Same CDS sequence) XM_017351778.1
    Accession Version XM_017351778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAATT AAGGAGAAAG TTAAACCAGC TTCTATAATG
    AAAGAGCCTG AAGTTGTGAA ACAAAAGACT CCTGAACCGA AACCCAAAAA AGAGCCTGAA
    GTTGTGAAAG AAAAGGCTCC TGAACCTAAA CCCAAAAAAG AGCCTGAAGT TGCTAAAGAG
    AAAGCCAAAC CAGCACCTCA GAAGAAAGAA GTCATCAAAG AAAAAGTCAA ACTTGCTCCT
    GTAAAGAAAG TGCCCGAAGT TGTCGAAGAA AAGGCACTTA AACCAGAACC CAAAAAAGAT
    GAAGTTGGTC TTATTGGTCT GGACCTGGAG CTCCTAAACT CCATCAACCA AAAACTGTCT
    CTCCTGGACT TGCTAAGGAA GGACATTGGA GAAATGAAAA GCGATCTGGA GTCCACTCAG
    AATCAAATCA CACTTTTACG GATGGACAAC AGAACGATCA AAGAGCCAGA GACTATTGGT
    GTGAAAGTAA AACCAGCAGT CAAGGAAAAA GCAAAAAGAG ACCACGCTCT GATCAATGCT
    ACTAGGACTG CACATATTAA AACAGAGCGT GAATCTCTAA AAAACATCAC GAAACCAGTG
    CCTGCAAAGA AAGCCAAAGA GGCTCCAAAG GTTAAGATTG CGCTGGAGCC TAAAAAGAAA
    GGAATCATCC CTGAGAAGGT CAAAGAAGTA AAGGAAACTT TACAGAAAGA ACCTGAGTTG
    GTCAAAGATA AAGTAGTTGA GCCAATAGAG AAGGAAATTG TTGTTGAGAA GGAAGTCAAA
    GGAGAAGAGC CTGAAATCAC CAAAGATATT CCAGAGGTGG AGGAAGAGGA CATTCCCTAC
    TTCCAGTGTT TCTTTGTGGA TGAGGATGAC ACTCAGTATC CGTTCTTCCC TTTCTCTCCA
    CCGCTTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207267.1
    CDS342..8333
    Translation

    Target ORF information:

    RefSeq Version XM_017351778.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GAAGCTGTAG TCATCAAAGA AAAAGCTGTA
    CCAGCCATCC TGAAGGAAGT GGCTGAAATT AAGGAGAAAG TTAAACCAGC TTCTATAATG
    AAAGAGCCTG AAGTTGTGAA ACAAAAGACT CCTGAACCGA AACCCAAAAA AGAGCCTGAA
    GTTGTGAAAG AAAAGGCTCC TGAACCTAAA CCCAAAAAAG AGCCTGAAGT TGCTAAAGAG
    AAAGCCAAAC CAGCACCTCA GAAGAAAGAA GTCATCAAAG AAAAAGTCAA ACTTGCTCCT
    GTAAAGAAAG TGCCCGAAGT TGTCGAAGAA AAGGCACTTA AACCAGAACC CAAAAAAGAT
    GAAGTTGGTC TTATTGGTCT GGACCTGGAG CTCCTAAACT CCATCAACCA AAAACTGTCT
    CTCCTGGACT TGCTAAGGAA GGACATTGGA GAAATGAAAA GCGATCTGGA GTCCACTCAG
    AATCAAATCA CACTTTTACG GATGGACAAC AGAACGATCA AAGAGCCAGA GACTATTGGT
    GTGAAAGTAA AACCAGCAGT CAAGGAAAAA GCAAAAAGAG ACCACGCTCT GATCAATGCT
    ACTAGGACTG CACATATTAA AACAGAGCGT GAATCTCTAA AAAACATCAC GAAACCAGTG
    CCTGCAAAGA AAGCCAAAGA GGCTCCAAAG GTTAAGATTG CGCTGGAGCC TAAAAAGAAA
    GGAATCATCC CTGAGAAGGT CAAAGAAGTA AAGGAAACTT TACAGAAAGA ACCTGAGTTG
    GTCAAAGATA AAGTAGTTGA GCCAATAGAG AAGGAAATTG TTGTTGAGAA GGAAGTCAAA
    GGAGAAGAGC CTGAAATCAC CAAAGATATT CCAGAGGTGG AGGAAGAGGA CATTCCCTAC
    TTCCAGTGTT TCTTTGTGGA TGAGGATGAC ACTCAGTATC CGTTCTTCCC TTTCTCTCCA
    CCGCTTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55697
    Clone ID Related Accession (Same CDS sequence) XM_017351779.1
    Accession Version XM_017351779.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGTAGC TGAAGTCATC
    AAAGACAGAA CTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGAAACCA
    AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA GTCATTGAAA AGAAAGTTAT ACCAGCTCCC
    GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA AAGGCTCCTG AACTGACAAC AAAAAAAGCA
    CCTGAGATAA CCAAAGAAAA ACCGAAGCCA GTACCAAAGA AAGAGGCTGA AGTCATTAAA
    GAGAAAGCTA AACTAGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAGGA GAAAGTTAAA
    CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT CATAAGGAAA AAGCACCTGA ACCAAAATCA
    GAACCTACAA AAAAAGTTGA GGTTCCAAAG GAAAAGGCAA AACCAGTTGC TAAAGAAAAG
    GAACCTGAAA TAACCAAAGA AAAGCCAAAA CCAGCACCAA AGAAAGAAGC TGTAGTCATC
    AAAGAAAAAG CTGTACCAGC CATCCTGAAG GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG
    AAGAAAGTGG CTGAAATTAA GGAGAAAGTT AAACCAGCTT CTATAATGAA AGAGCCTGAA
    GTTGTGAAAC AAAAGACTCC TGAACCGAAA CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA
    AAGGCTCCTG AACCTAAACC CAAAAAAGAG CCTGAAGTTG CTAAAGAGAA AGCCAAACCA
    GCACCTCAGA AGAAAGAAGT CATCAAAGAA AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG
    CCCGAAGTTG TCGAAGAAAA GGCACTTAAA CCAGAACCCA AAAAAGATGA AGTTGGTCTT
    ATTGGTCTGG ACCTGGAGCT CCTAAACTCC ATCAACCAAA AACTGTCTCT CCTGGACTTG
    CTAAGGAAGG ACATTGGAGA AATGAAAAGC GATCTGGAGT CCACTCAGAA TCAAATCACA
    CTTTTACGGA TGGACAACAG AACGATCAAA GAGCCAGAGA CTATTGGTGT GAAAGTAAAA
    CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC CACGCTCTGA TCAATGCTAC TAGGACTGCA
    CATATTAAAA CAGAGCGTGA ATCTCTAAAA AACATCACGA AACCAGTGCC TGCAAAGAAA
    GCCAAAGAGG CTCCAAAGGT TAAGATTGCG CTGGAGCCTA AAAAGAAAGG AATCATCCCT
    GAGAAGGTCA AAGAAGTAAA GGAAACTTTA CAGAAAGAAC CTGAGTTGGT CAAAGATAAA
    GTAGTTGAGC CAATAGAGAA GGAAATTGTT GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT
    GAAATCACCA AAGATATTCC AGAGGTGGAG GAAGAGGACA TTCCCTACTT CCAGTGTTTC
    TTTGTGGATG AGGATGACAC TCAGTATCCG TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207268.1
    CDS343..8322
    Translation

    Target ORF information:

    RefSeq Version XM_017351779.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGTAGC TGAAGTCATC
    AAAGACAGAA CTAAACCAGT CCCTGAGAAG AAAGAGGCTG AAGTCATCAA GGAGAAACCA
    AAAGCAGTTC CCGAGAAGAA AGAGGCTAAA GTCATTGAAA AGAAAGTTAT ACCAGCTCCC
    GTAAAGAAAG AGCCTGAAGT TGTTAAGGAA AAGGCTCCTG AACTGACAAC AAAAAAAGCA
    CCTGAGATAA CCAAAGAAAA ACCGAAGCCA GTACCAAAGA AAGAGGCTGA AGTCATTAAA
    GAGAAAGCTA AACTAGCTCC TGAAAAGAAA GAGGTTGAAG TCATCAAGGA GAAAGTTAAA
    CCAGCTCCGT TAAAGAAAGA GCCTGAGGTT CATAAGGAAA AAGCACCTGA ACCAAAATCA
    GAACCTACAA AAAAAGTTGA GGTTCCAAAG GAAAAGGCAA AACCAGTTGC TAAAGAAAAG
    GAACCTGAAA TAACCAAAGA AAAGCCAAAA CCAGCACCAA AGAAAGAAGC TGTAGTCATC
    AAAGAAAAAG CTGTACCAGC CATCCTGAAG GAAGTAAAGA AAGTTAAAGC AGCTCCTGAG
    AAGAAAGTGG CTGAAATTAA GGAGAAAGTT AAACCAGCTT CTATAATGAA AGAGCCTGAA
    GTTGTGAAAC AAAAGACTCC TGAACCGAAA CCCAAAAAAG AGCCTGAAGT TGTGAAAGAA
    AAGGCTCCTG AACCTAAACC CAAAAAAGAG CCTGAAGTTG CTAAAGAGAA AGCCAAACCA
    GCACCTCAGA AGAAAGAAGT CATCAAAGAA AAAGTCAAAC TTGCTCCTGT AAAGAAAGTG
    CCCGAAGTTG TCGAAGAAAA GGCACTTAAA CCAGAACCCA AAAAAGATGA AGTTGGTCTT
    ATTGGTCTGG ACCTGGAGCT CCTAAACTCC ATCAACCAAA AACTGTCTCT CCTGGACTTG
    CTAAGGAAGG ACATTGGAGA AATGAAAAGC GATCTGGAGT CCACTCAGAA TCAAATCACA
    CTTTTACGGA TGGACAACAG AACGATCAAA GAGCCAGAGA CTATTGGTGT GAAAGTAAAA
    CCAGCAGTCA AGGAAAAAGC AAAAAGAGAC CACGCTCTGA TCAATGCTAC TAGGACTGCA
    CATATTAAAA CAGAGCGTGA ATCTCTAAAA AACATCACGA AACCAGTGCC TGCAAAGAAA
    GCCAAAGAGG CTCCAAAGGT TAAGATTGCG CTGGAGCCTA AAAAGAAAGG AATCATCCCT
    GAGAAGGTCA AAGAAGTAAA GGAAACTTTA CAGAAAGAAC CTGAGTTGGT CAAAGATAAA
    GTAGTTGAGC CAATAGAGAA GGAAATTGTT GTTGAGAAGG AAGTCAAAGG AGAAGAGCCT
    GAAATCACCA AAGATATTCC AGAGGTGGAG GAAGAGGACA TTCCCTACTT CCAGTGTTTC
    TTTGTGGATG AGGATGACAC TCAGTATCCG TTCTTCCCTT TCTCTCCACC GCTTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55698
    Clone ID Related Accession (Same CDS sequence) XM_017351780.1
    Accession Version XM_017351780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GTGGCTGAAA TTAAGGAGAA AGTTAAACCA
    GCTTCTATAA TGAAAGAGCC TGAAGTTGTG AAACAAAAGA CTCCTGAACC GAAACCCAAA
    AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT CCTGAACCTA AACCCAAAAA AGAGCCTGAA
    GTTGCTAAAG AGAAAGCCAA ACCAGCACCT CAGAAGAAAG AAGTCATCAA AGAAAAAGTC
    AAACTTGCTC CTGTAAAGAA AGTGCCCGAA GTTGTCGAAG AAAAGGCACT TAAACCAGAA
    CCCAAAAAAG ATGAAGTTGG TCTTATTGGT CTGGACCTGG AGCTCCTAAA CTCCATCAAC
    CAAAAACTGT CTCTCCTGGA CTTGCTAAGG AAGGACATTG GAGAAATGAA AAGCGATCTG
    GAGTCCACTC AGAATCAAAT CACACTTTTA CGGATGGACA ACAGAACGAT CAAAGAGCCA
    GAGACTATTG GTGTGAAAGT AAAACCAGCA GTCAAGGAAA AAGCAAAAAG AGACCACGCT
    CTGATCAATG CTACTAGGAC TGCACATATT AAAACAGAGC GTGAATCTCT AAAAAACATC
    ACGAAACCAG TGCCTGCAAA GAAAGCCAAA GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG
    CCTAAAAAGA AAGGAATCAT CCCTGAGAAG GTCAAAGAAG TAAAGGAAAC TTTACAGAAA
    GAACCTGAGT TGGTCAAAGA TAAAGTAGTT GAGCCAATAG AGAAGGAAAT TGTTGTTGAG
    AAGGAAGTCA AAGGAGAAGA GCCTGAAATC ACCAAAGATA TTCCAGAGGT GGAGGAAGAG
    GACATTCCCT ACTTCCAGTG TTTCTTTGTG GATGAGGATG ACACTCAGTA TCCGTTCTTC
    CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207269.1
    CDS342..8285
    Translation

    Target ORF information:

    RefSeq Version XM_017351780.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGCACC CGAAATAACC
    AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA GTAGCTGAAG TCATCAAAGA CAGAACTAAA
    CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC ATCAAGGAGA AACCAAAAGC AGTTCCCGAG
    AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA GTTATACCAG CTCCCGTAAA GAAAGAGCCT
    GAAGTTGTTA AGGAAAAGGC TCCTGAACTG ACAACAAAAA AAGCACCTGA GATAACCAAA
    GAAAAACCGA AGCCAGTACC AAAGAAAGAG GCTGAAGTCA TTAAAGAGAA AGCTAAACTA
    GCTCCTGAAA AGAAAGAGGT TGAAGTCATC AAGGAGAAAG TTAAACCAGC TCCGTTAAAG
    AAAGAGCCTG AGGTTCATAA GGAAAAAGCA CCTGAACCAA AATCAGAACC TACAAAAAAA
    GTTGAGGTTC CAAAGGAAAA GGCAAAACCA GTTGCTAAAG AAAAGGAACC TGAAATAACC
    AAAGAAAAGC CAAAACCAGC ACCAAAGAAA GTGGCTGAAA TTAAGGAGAA AGTTAAACCA
    GCTTCTATAA TGAAAGAGCC TGAAGTTGTG AAACAAAAGA CTCCTGAACC GAAACCCAAA
    AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT CCTGAACCTA AACCCAAAAA AGAGCCTGAA
    GTTGCTAAAG AGAAAGCCAA ACCAGCACCT CAGAAGAAAG AAGTCATCAA AGAAAAAGTC
    AAACTTGCTC CTGTAAAGAA AGTGCCCGAA GTTGTCGAAG AAAAGGCACT TAAACCAGAA
    CCCAAAAAAG ATGAAGTTGG TCTTATTGGT CTGGACCTGG AGCTCCTAAA CTCCATCAAC
    CAAAAACTGT CTCTCCTGGA CTTGCTAAGG AAGGACATTG GAGAAATGAA AAGCGATCTG
    GAGTCCACTC AGAATCAAAT CACACTTTTA CGGATGGACA ACAGAACGAT CAAAGAGCCA
    GAGACTATTG GTGTGAAAGT AAAACCAGCA GTCAAGGAAA AAGCAAAAAG AGACCACGCT
    CTGATCAATG CTACTAGGAC TGCACATATT AAAACAGAGC GTGAATCTCT AAAAAACATC
    ACGAAACCAG TGCCTGCAAA GAAAGCCAAA GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG
    CCTAAAAAGA AAGGAATCAT CCCTGAGAAG GTCAAAGAAG TAAAGGAAAC TTTACAGAAA
    GAACCTGAGT TGGTCAAAGA TAAAGTAGTT GAGCCAATAG AGAAGGAAAT TGTTGTTGAG
    AAGGAAGTCA AAGGAGAAGA GCCTGAAATC ACCAAAGATA TTCCAGAGGT GGAGGAAGAG
    GACATTCCCT ACTTCCAGTG TTTCTTTGTG GATGAGGATG ACACTCAGTA TCCGTTCTTC
    CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55699
    Clone ID Related Accession (Same CDS sequence) XM_017351781.1
    Accession Version XM_017351781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG AAAGAGGCTA AAGTCATTGA AAAGAAAGTT
    ATACCAGCTC CCGTAAAGAA AGAGCCTGAA GTTGTTAAGG AAAAGGCTCC TGAACTGACA
    ACAAAAAAAG CACCTGAGAT AACCAAAGAA AAACCGAAGC CAGTACCAAA GAAAGAGGCT
    GAAGTCATTA AAGAGAAAGC TAAACTAGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAG
    GAGAAAGTTA AACCAGCTCC GTTAAAGAAA GAGCCTGAGG TTCATAAGGA AAAAGCACCT
    GAACCAAAAT CAGAACCTAC AAAAAAAGTT GAGGTTCCAA AGGAAAAGGC AAAACCAGTT
    GCTAAAGAAA AGGAACCTGA AATAACCAAA GAAAAGCCAA AACCAGCACC AAAGAAAGAA
    GCTGTAGTCA TCAAAGAAAA AGCTGTACCA GCCATCCTGA AGGAAGTAAA GAAAGTTAAA
    GCAGCTCCTG AGAAGAAAGT GGCTGAAATT AAGGAGAAAG TTAAACCAGC TTCTATAATG
    AAAGAGCCTG AAGTTGTGAA ACAAAAGACT CCTGAACCGA AACCCAAAAA AGAGCCTGAA
    GTTGTGAAAG AAAAGGCTCC TGAACCTAAA CCCAAAAAAG AGCCTGAAGT TGCTAAAGAG
    AAAGCCAAAC CAGCACCTCA GAAGAAAGAA GTCATCAAAG AAAAAGTCAA ACTTGCTCCT
    GTAAAGAAAG TGCCCGAAGT TGTCGAAGAA AAGGCACTTA AACCAGAACC CAAAAAAGAT
    GAAGTTGGTC TTATTGGTCT GGACCTGGAG CTCCTAAACT CCATCAACCA AAAACTGTCT
    CTCCTGGACT TGCTAAGGAA GGACATTGGA GAAATGAAAA GCGATCTGGA GTCCACTCAG
    AATCAAATCA CACTTTTACG GATGGACAAC AGAACGATCA AAGAGCCAGA GACTATTGGT
    GTGAAAGTAA AACCAGCAGT CAAGGAAAAA GCAAAAAGAG ACCACGCTCT GATCAATGCT
    ACTAGGACTG CACATATTAA AACAGAGCGT GAATCTCTAA AAAACATCAC GAAACCAGTG
    CCTGCAAAGA AAGCCAAAGA GGCTCCAAAG GTTAAGATTG CGCTGGAGCC TAAAAAGAAA
    GGAATCATCC CTGAGAAGGT CAAAGAAGTA AAGGAAACTT TACAGAAAGA ACCTGAGTTG
    GTCAAAGATA AAGTAGTTGA GCCAATAGAG AAGGAAATTG TTGTTGAGAA GGAAGTCAAA
    GGAGAAGAGC CTGAAATCAC CAAAGATATT CCAGAGGTGG AGGAAGAGGA CATTCCCTAC
    TTCCAGTGTT TCTTTGTGGA TGAGGATGAC ACTCAGTATC CGTTCTTCCC TTTCTCTCCA
    CCGCTTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207270.1
    CDS343..8274
    Translation

    Target ORF information:

    RefSeq Version XM_017351781.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATAAAGG AGAGAATTAA ACCTGCTCCT
    GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC CAAAAAAGAA
    CCTGAAGTTA TCAAAGAGAA AGTGAAACCA GCGCCAGAGA AGAAAGAGGC TGAAGTCATC
    AAGGAGAAAC CAAAAGCAGT TCCCGAGAAG AAAGAGGCTA AAGTCATTGA AAAGAAAGTT
    ATACCAGCTC CCGTAAAGAA AGAGCCTGAA GTTGTTAAGG AAAAGGCTCC TGAACTGACA
    ACAAAAAAAG CACCTGAGAT AACCAAAGAA AAACCGAAGC CAGTACCAAA GAAAGAGGCT
    GAAGTCATTA AAGAGAAAGC TAAACTAGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAG
    GAGAAAGTTA AACCAGCTCC GTTAAAGAAA GAGCCTGAGG TTCATAAGGA AAAAGCACCT
    GAACCAAAAT CAGAACCTAC AAAAAAAGTT GAGGTTCCAA AGGAAAAGGC AAAACCAGTT
    GCTAAAGAAA AGGAACCTGA AATAACCAAA GAAAAGCCAA AACCAGCACC AAAGAAAGAA
    GCTGTAGTCA TCAAAGAAAA AGCTGTACCA GCCATCCTGA AGGAAGTAAA GAAAGTTAAA
    GCAGCTCCTG AGAAGAAAGT GGCTGAAATT AAGGAGAAAG TTAAACCAGC TTCTATAATG
    AAAGAGCCTG AAGTTGTGAA ACAAAAGACT CCTGAACCGA AACCCAAAAA AGAGCCTGAA
    GTTGTGAAAG AAAAGGCTCC TGAACCTAAA CCCAAAAAAG AGCCTGAAGT TGCTAAAGAG
    AAAGCCAAAC CAGCACCTCA GAAGAAAGAA GTCATCAAAG AAAAAGTCAA ACTTGCTCCT
    GTAAAGAAAG TGCCCGAAGT TGTCGAAGAA AAGGCACTTA AACCAGAACC CAAAAAAGAT
    GAAGTTGGTC TTATTGGTCT GGACCTGGAG CTCCTAAACT CCATCAACCA AAAACTGTCT
    CTCCTGGACT TGCTAAGGAA GGACATTGGA GAAATGAAAA GCGATCTGGA GTCCACTCAG
    AATCAAATCA CACTTTTACG GATGGACAAC AGAACGATCA AAGAGCCAGA GACTATTGGT
    GTGAAAGTAA AACCAGCAGT CAAGGAAAAA GCAAAAAGAG ACCACGCTCT GATCAATGCT
    ACTAGGACTG CACATATTAA AACAGAGCGT GAATCTCTAA AAAACATCAC GAAACCAGTG
    CCTGCAAAGA AAGCCAAAGA GGCTCCAAAG GTTAAGATTG CGCTGGAGCC TAAAAAGAAA
    GGAATCATCC CTGAGAAGGT CAAAGAAGTA AAGGAAACTT TACAGAAAGA ACCTGAGTTG
    GTCAAAGATA AAGTAGTTGA GCCAATAGAG AAGGAAATTG TTGTTGAGAA GGAAGTCAAA
    GGAGAAGAGC CTGAAATCAC CAAAGATATT CCAGAGGTGG AGGAAGAGGA CATTCCCTAC
    TTCCAGTGTT TCTTTGTGGA TGAGGATGAC ACTCAGTATC CGTTCTTCCC TTTCTCTCCA
    CCGCTTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55700
    Clone ID Related Accession (Same CDS sequence) XM_017351782.1
    Accession Version XM_017351782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGGGGA TGCAGACATC
    ACTGAAACAG CCAAACTTGG AGGCACTGTA TTGGATACAG TTACTGACTG GATTCGATTG
    CCAGTGGGAT ACTTATTTTA TTTATTTGAA GCCATCCTGG AGGTAACCAT TATTTATCCA
    GCAGATATGG CAAGTGAAGC ATTTTTGCAA ATTTTAAATG TGATCAAGAA CACTTCATCA
    GCCATATTTG GGTTTCTTGA AGCTTGGTTC CCCAAAATGA GCACTCGTCC TACAGAGCTG
    GTTGAAAAGG TTGCAGAGGA GGCCACTGAT GTAAAGACCA CCGTCTCTAA TTATCTGTCC
    AGTCTGTTTG TGAGGGATGA AGGTGGTATT CCTGACGTCA CTTTTGATCC CATGAAAGTT
    GTCTCAGACA CCGTTGAGGA GTTTGCAGAC AGAAGAGATA TGTTATTAGC TTATCTCTCA
    AACATGCTCA TGGAAGACAA AGTTTCAGAT GAAACACCAC AGGTGATAAG GCGAAAAGGA
    GAATTTCTGC CCCCTATGGA AAAAGTTGCA GAAGTACTAG GAAAAACTCA GGACCCCATC
    TACGCTGCCT ACATCGCTGT GAGATCCACA GCTGAGACAG ACGGCGATGA ACTGGAAATA
    AAGGGGGCTG AGGTGGACTC TGCTGAAGAT ATCTTGGAAG AACAAAAAGT TGAAAAGACA
    GTGGACGGCT CTGAAAATGA TGCAGTTGCC TCTGTTGAGG AGGAAAAAGC AGTTGTTGAT
    ATTGTAGATG AGGAATCTTC TCATACGCCT GAAGTCACAG ATGAAGAAAT GGCAAAAGAA
    AAAGATGAAG ACACTTCTGA TTCAACTGAA ATGACTTCTG ATGTGCAAGA CATTGATGAT
    ACCATAGATT CTGAGACTGA AGAAATTCCT GAAATGGTTG AAAGTGTTTC AGAGGTTGGA
    GAAGAGGAAC CTTTTGTAAT TGAAAAGGTT GAAGACACCT CTGATTCGAA AGAAATCACT
    TCTAATGCCG CAGATGTGCA AGATGTTGAT GATAATTCTG ATGTTGATAC TGAAGAAATT
    CCTGAATTGG TTGGAGGTGT TTCAGATGTT GGTGAAGAGG AACCTTCTGA AGTCATAGAT
    GAAGAAATTG CAACTGAAAA GCTTGAAGAC GCCTCTGATT CAACTGGATT GATTCCCAGT
    GCTGAAAATG TGAAAGACAC TGACGATATC TTAGAAGTTG ACGCAGAGGT GAATGAAGTT
    GAAACAGTTG AAGACATTTC AGAAGTTACA AATGAGGAAA CTTCTGATGT GCCAGAAGTC
    ATAGATGAGG AAAAGGTTGA AAAGTCTGAT GATATCTTTC ATTTTGCAGA GGACAAACCA
    ACTGAATTGA TTTCTGATGC TCCAGATGTA ACAGAAGACA TTGAAATCTC AATCTCTGCA
    GTTTCAGAGG ATAATGAAAC AATTCAAGAA GCTGATATGA CAGATGTCAA AGATGAGGAA
    ATGGAAGCTG AAAAGGTTGA TGAAATCTCC TATTTTGTAG AGGATAAATC AACTGAAATT
    ACTTCTGATG CTACAGATGT GAAAGAAGAC ACTGATGGCT TTACAGTTAC AGAGGAGAAA
    GGAACAATTG AAAGCCTTCA AGAAGCAGCA ATGAAAATGT CGGGAAATGC AGATGATGAA
    ATCCCCAATG TCACAGAGAT GGTTGAAGAA CCTGATACTT CTGATGTTAT GGATGAGCTA
    GACGAACCTG AAAAAGATGA AGATGTAATC AGTGTCACCA ATGAAAGGGT TACAACTGCA
    CAAGATGTCA TATTTTCAAA GGAAAAAATG GGAGCAGCAG AACTGGATGA TCATGACAAA
    CTCAGGGCTA AGGATGATTC AGAAGTGGAT CACATGGAGA AGGAAATTGC AGATGCTGAA
    AATGACCTTC AGAATGAGAT AGATAAAGAT GAGTTTAAGG TTGAAGATGC ATCTCATCAA
    ACTATTTTGG GAAGCACTGC TGCTGAAGAA GACCAAGATG ATGATGATGA TGATGAAGAA
    CCTGTCCTTC TTACTGTCAA ACATGAGCTC ACAGACGGTG ATGATGAAAA TAATAATAAC
    AACGACAGAA AGAGAGATGC CAGAACTAAA GGTCTGATCA ATAGAAAAAC ACCAAAGAGC
    CATTCCACTG AAACTGATGA ACAAAAGGCG CACAAGGATC TTCATAAAGA GTCTGCTAAA
    GTTCTTGAGC AAGTGAAAGA AGCCAAAGAG AAAAAAGCTA GAGAGGAGAT ATACAAAATC
    ATTCAAGAAA TGAGGGAGGA GAAGGCTGAA GGTGTGGAGG AAGAGATCGT GTTACAACAG
    ATAAACATAA CAGTAGAAAA GGTCAAGAAA GAAGAAACTG TCAAAAAGGA GGAAAAGGCT
    AAAAGAAAAG AAAAGGCTCA TAAACCAAAG GATGCAAAGA TTGCCAAAGA CAAACCTAAA
    GCAGTCCCAG TAAAGCAAGA GGATGAAGTA GCCAAGAAAG AAACCAAACC AGCACCATCT
    AAGAAAGCAG AGGCTAAAAT TGTGAAGGAG AAACCCAAAG CACATCCTGC AAAGAAAGAA
    AAAGCTAAAG ATATTAAGGA AAAGGCAAAA TCTGGTCAAA AAGAAAAAGT TGCTGAGGCT
    GTCAAGGAAA AATCGAAGCC AGTAAAGAAA GAAGCTGAAA AAATTGATGC CAAAGTCAAA
    CCAGCAGCAG AAAAGAAAGT GGATGAAGCC CCCAAAGAGA AACCTAAAGT GAAGAAAGAG
    GCTGAAGTAA TCAAAAAGAT TAAACTAGAG CCTGAGAGGA AAGAAGCTAA AACTCCCAAG
    GCCAAAATTG AACCAGCTGG TTTGAAGAAA GATGCTGAAG CTGCTAAGGA GAAAGCAAAA
    CCTGCTCCAG TCAAGAAAGG GCCTGAGGTT TCCAGAGAAA AAGCCAAACC AGAGCCCGCA
    AAAAAAGAGC TTGAAGCGGT CAAAGAGAAA GCAAAACCAG CACCAGCTAA AAAAGATGAA
    GTCAAAGAGA AAGAAAAACC AGCTCCTGTA AAGAAAGCTG AAGAAATTAA AAAGGCAAAA
    GCAGCTCCTT CAAGGAAAGA AGCTGTAAAG GAAAAACCCA AACCTGTTTC CGAAAAGAAA
    GAACCTGCTG TTGCAGTTGA AAAAGCCAAG CCAAAATCTA CAAAGAAAGA AGCTGAAGTC
    GAGGAAAAAG TGAAACCTGC TCCTGAGAAG AAAGAAGCTG AAGTCATCAT GGTGAAAACT
    AAACCAGTTC TTAAAAAGAA AGAGGCTGAA GTAATCAAAG AGGTTAAACC AGCCCTTGAG
    AAGAAAGAGG CTGAAGTCAT CAAGGAGAAA GTTAAACCAG CTCCTGTAAA GAAAGAGCCT
    GAAGTTGTTA AAGAAAAGGA ACCTGAACCA AAACCCAAAA GAGAGCCAGA AATTATTAAG
    GAGAAATCAG CACCACCAAA GAAAGTTGAG GTTCCAAAAC AAAAGGCTCC TGAAAAGAAA
    GCACCTGAAA TAACCAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGCTGTA
    AAAGAGAAAG CTAAACCAGA GAAGAAAGAG GCTGAAGCCG TAAAGGAGAA AACGAAACCA
    GCCCCTGAGA AAGAGACTGA AATAATCAAG GATAAAATTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGAAGGA AGCAAAACCA GCTCCTGTAA AGAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAACCAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCT CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207271.1
    CDS342..8255
    Translation

    Target ORF information:

    RefSeq Version XM_017351782.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGGGGA TGCAGACATC
    ACTGAAACAG CCAAACTTGG AGGCACTGTA TTGGATACAG TTACTGACTG GATTCGATTG
    CCAGTGGGAT ACTTATTTTA TTTATTTGAA GCCATCCTGG AGGTAACCAT TATTTATCCA
    GCAGATATGG CAAGTGAAGC ATTTTTGCAA ATTTTAAATG TGATCAAGAA CACTTCATCA
    GCCATATTTG GGTTTCTTGA AGCTTGGTTC CCCAAAATGA GCACTCGTCC TACAGAGCTG
    GTTGAAAAGG TTGCAGAGGA GGCCACTGAT GTAAAGACCA CCGTCTCTAA TTATCTGTCC
    AGTCTGTTTG TGAGGGATGA AGGTGGTATT CCTGACGTCA CTTTTGATCC CATGAAAGTT
    GTCTCAGACA CCGTTGAGGA GTTTGCAGAC AGAAGAGATA TGTTATTAGC TTATCTCTCA
    AACATGCTCA TGGAAGACAA AGTTTCAGAT GAAACACCAC AGGTGATAAG GCGAAAAGGA
    GAATTTCTGC CCCCTATGGA AAAAGTTGCA GAAGTACTAG GAAAAACTCA GGACCCCATC
    TACGCTGCCT ACATCGCTGT GAGATCCACA GCTGAGACAG ACGGCGATGA ACTGGAAATA
    AAGGGGGCTG AGGTGGACTC TGCTGAAGAT ATCTTGGAAG AACAAAAAGT TGAAAAGACA
    GTGGACGGCT CTGAAAATGA TGCAGTTGCC TCTGTTGAGG AGGAAAAAGC AGTTGTTGAT
    ATTGTAGATG AGGAATCTTC TCATACGCCT GAAGTCACAG ATGAAGAAAT GGCAAAAGAA
    AAAGATGAAG ACACTTCTGA TTCAACTGAA ATGACTTCTG ATGTGCAAGA CATTGATGAT
    ACCATAGATT CTGAGACTGA AGAAATTCCT GAAATGGTTG AAAGTGTTTC AGAGGTTGGA
    GAAGAGGAAC CTTTTGTAAT TGAAAAGGTT GAAGACACCT CTGATTCGAA AGAAATCACT
    TCTAATGCCG CAGATGTGCA AGATGTTGAT GATAATTCTG ATGTTGATAC TGAAGAAATT
    CCTGAATTGG TTGGAGGTGT TTCAGATGTT GGTGAAGAGG AACCTTCTGA AGTCATAGAT
    GAAGAAATTG CAACTGAAAA GCTTGAAGAC GCCTCTGATT CAACTGGATT GATTCCCAGT
    GCTGAAAATG TGAAAGACAC TGACGATATC TTAGAAGTTG ACGCAGAGGT GAATGAAGTT
    GAAACAGTTG AAGACATTTC AGAAGTTACA AATGAGGAAA CTTCTGATGT GCCAGAAGTC
    ATAGATGAGG AAAAGGTTGA AAAGTCTGAT GATATCTTTC ATTTTGCAGA GGACAAACCA
    ACTGAATTGA TTTCTGATGC TCCAGATGTA ACAGAAGACA TTGAAATCTC AATCTCTGCA
    GTTTCAGAGG ATAATGAAAC AATTCAAGAA GCTGATATGA CAGATGTCAA AGATGAGGAA
    ATGGAAGCTG AAAAGGTTGA TGAAATCTCC TATTTTGTAG AGGATAAATC AACTGAAATT
    ACTTCTGATG CTACAGATGT GAAAGAAGAC ACTGATGGCT TTACAGTTAC AGAGGAGAAA
    GGAACAATTG AAAGCCTTCA AGAAGCAGCA ATGAAAATGT CGGGAAATGC AGATGATGAA
    ATCCCCAATG TCACAGAGAT GGTTGAAGAA CCTGATACTT CTGATGTTAT GGATGAGCTA
    GACGAACCTG AAAAAGATGA AGATGTAATC AGTGTCACCA ATGAAAGGGT TACAACTGCA
    CAAGATGTCA TATTTTCAAA GGAAAAAATG GGAGCAGCAG AACTGGATGA TCATGACAAA
    CTCAGGGCTA AGGATGATTC AGAAGTGGAT CACATGGAGA AGGAAATTGC AGATGCTGAA
    AATGACCTTC AGAATGAGAT AGATAAAGAT GAGTTTAAGG TTGAAGATGC ATCTCATCAA
    ACTATTTTGG GAAGCACTGC TGCTGAAGAA GACCAAGATG ATGATGATGA TGATGAAGAA
    CCTGTCCTTC TTACTGTCAA ACATGAGCTC ACAGACGGTG ATGATGAAAA TAATAATAAC
    AACGACAGAA AGAGAGATGC CAGAACTAAA GGTCTGATCA ATAGAAAAAC ACCAAAGAGC
    CATTCCACTG AAACTGATGA ACAAAAGGCG CACAAGGATC TTCATAAAGA GTCTGCTAAA
    GTTCTTGAGC AAGTGAAAGA AGCCAAAGAG AAAAAAGCTA GAGAGGAGAT ATACAAAATC
    ATTCAAGAAA TGAGGGAGGA GAAGGCTGAA GGTGTGGAGG AAGAGATCGT GTTACAACAG
    ATAAACATAA CAGTAGAAAA GGTCAAGAAA GAAGAAACTG TCAAAAAGGA GGAAAAGGCT
    AAAAGAAAAG AAAAGGCTCA TAAACCAAAG GATGCAAAGA TTGCCAAAGA CAAACCTAAA
    GCAGTCCCAG TAAAGCAAGA GGATGAAGTA GCCAAGAAAG AAACCAAACC AGCACCATCT
    AAGAAAGCAG AGGCTAAAAT TGTGAAGGAG AAACCCAAAG CACATCCTGC AAAGAAAGAA
    AAAGCTAAAG ATATTAAGGA AAAGGCAAAA TCTGGTCAAA AAGAAAAAGT TGCTGAGGCT
    GTCAAGGAAA AATCGAAGCC AGTAAAGAAA GAAGCTGAAA AAATTGATGC CAAAGTCAAA
    CCAGCAGCAG AAAAGAAAGT GGATGAAGCC CCCAAAGAGA AACCTAAAGT GAAGAAAGAG
    GCTGAAGTAA TCAAAAAGAT TAAACTAGAG CCTGAGAGGA AAGAAGCTAA AACTCCCAAG
    GCCAAAATTG AACCAGCTGG TTTGAAGAAA GATGCTGAAG CTGCTAAGGA GAAAGCAAAA
    CCTGCTCCAG TCAAGAAAGG GCCTGAGGTT TCCAGAGAAA AAGCCAAACC AGAGCCCGCA
    AAAAAAGAGC TTGAAGCGGT CAAAGAGAAA GCAAAACCAG CACCAGCTAA AAAAGATGAA
    GTCAAAGAGA AAGAAAAACC AGCTCCTGTA AAGAAAGCTG AAGAAATTAA AAAGGCAAAA
    GCAGCTCCTT CAAGGAAAGA AGCTGTAAAG GAAAAACCCA AACCTGTTTC CGAAAAGAAA
    GAACCTGCTG TTGCAGTTGA AAAAGCCAAG CCAAAATCTA CAAAGAAAGA AGCTGAAGTC
    GAGGAAAAAG TGAAACCTGC TCCTGAGAAG AAAGAAGCTG AAGTCATCAT GGTGAAAACT
    AAACCAGTTC TTAAAAAGAA AGAGGCTGAA GTAATCAAAG AGGTTAAACC AGCCCTTGAG
    AAGAAAGAGG CTGAAGTCAT CAAGGAGAAA GTTAAACCAG CTCCTGTAAA GAAAGAGCCT
    GAAGTTGTTA AAGAAAAGGA ACCTGAACCA AAACCCAAAA GAGAGCCAGA AATTATTAAG
    GAGAAATCAG CACCACCAAA GAAAGTTGAG GTTCCAAAAC AAAAGGCTCC TGAAAAGAAA
    GCACCTGAAA TAACCAAAGA AAAACCAAAG CCAGCACCAA AGAAAGAGGC TGAAGCTGTA
    AAAGAGAAAG CTAAACCAGA GAAGAAAGAG GCTGAAGCCG TAAAGGAGAA AACGAAACCA
    GCCCCTGAGA AAGAGACTGA AATAATCAAG GATAAAATTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGAAGGA AGCAAAACCA GCTCCTGTAA AGAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAACCAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCT CTGAGAAGAA AGAGGCTGAA ATAATTAAGG AGAAAGTTAA AGCAGCTCCT
    GTGAAGAAAG AAGTTGAAGT CAAGGAGGAA GCAAAACCAG CTCCTGTAAA AAAAGAAACT
    GAAGTCATCA AAGAAAAAGA AAAAACAGCT CCTGAGAAGA AAGAGGCTGA AGCTGTAAAG
    GAGAAAACGA AACCAACCCC TGAGAAGAAA GAGGCTGAAG TCATCAAGGA GATAGTTAAA
    CAAGCTCCTG TGAAGAAAGA GGCTGAACCT GTAAAGGAGA TAACTAAACC AGCCCCTGAG
    AAGAAAGAGG CTGAAATAAT CAAGGAGAAA GTTAAAGCAG CACCTCTGAA GAAAGAAGTC
    AAGGAGAAAG CAAAACCAGC TCCTGTAAAG AAAGAAGCTG AAGTCATCAA ACAAAAAGCC
    AAACCAGCTC CTGAGAAGAA AGAGGCCAAA GTCATCAAGG AGAAAGTTAA ACCGGCTCCT
    GTAAAGATAG AGGCTAAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA GGTCAAATCA GCTCCTGTGA AGAAAGAAGT TGAAGTCAAG
    GAGGAAGCAA AACCAGCTCC TGAAAAGAAA GAGGCTGAAG TCATCAAGGA GAAAGTTAAA
    CCTGCTCCTG TGAGGAAAGA AGCTGAAGAA ATAAAGGAAA AAGCTGAAGC AGTTTATGAG
    AAAAAAGAGG CTGAAGTAGC CAAGGAGATA GTTAAACCAA CTCCTTTAAA GAAGGAGCCC
    GAAGTTGTGA AGGAAAAGGC TCCTGAATCA AAACCCCAAA AAGAGGCTGA AATTATCAAA
    GAGAAAGTTA AACCTGCACC TGTAAAGAAA GAGCCTGAAG TTGCAAAAGT ACCAAAACCC
    AAAAAAGAGG CTGAACTCGC CAAAGAGAAA GTTAAACCTG CACCTGTAAA GAAAGAAGAA
    AAACCTAAGG CAGCACCAAA GAAAGAGGCT GAAGCTATTA AGGAGAAACG TACACCAGCT
    CCTGAAAAGA AAGTGGTAGA AGTCATCAAG GAGAAAGTCA AACCAGCCCC TGAGAAAAAA
    GCGGTTCTCA AGGAGAAAGT TAGACCTGCT CCTGAAAAGA AAGAGGTTGA AGTCATCAAA
    GAGAAAGCTA AACCAGCCCT TAAGAAGAAA GAGCCTAAAG TCAAGGAAAA GGTTCAACCA
    GCTCCTATAA AGAAAGAGCC AGAGCTTCCC AAAGAAAAGG TGCCTGAACC AACACCAGAA
    CCTGAGAGAG TTGAAGCTCC AAAAGAAAAG GCAAAAGTAA TCCCTAGGGA AAAAGTAGCT
    GAGATTACTA AAGAAAAACC AAAGCCAGCA CCAAAGAAAG AGGCTGAAGT AAAGGAGAAA
    GCTACACCTG CTCCTGTGCA CAAAGAGGTT GAAGTGTTCA AGGAGAAAGT TAAACAAGCT
    CCTGTAAAGA AAGAGCCAGA AGTTCCTAAA GAAAAGGCTC CTGAACCAAA ACCTAAAAAG
    ACTGAAGTCA TCAAAGCGAA AGTAAAACCA GCCCCTGAGA AAAAAGAGGC TGAAGTGAAG
    GAGAAAATTA AACCAGTCCC TGAGAAGAAA GAGGCTGAAG TCATAAAGGA GAGAATTAAA
    CCTGCTCCTG TAAAGAAAGA GCCTGAAGTT CCTAAAGAAA AGGCTCCTGA ACCAAAACCC
    AAAAAAGAAC CTGAAGTTAT CAAAGAGAAA GTGAAACCAG CGCCAGAGAA GAAAGCACCC
    GAAATAACCA AAGAAAAACC AAAGCCAGCA GCAAAGAAAG TAGCTGAAGT CATCAAAGAC
    AGAACTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAA ACCAAAAGCA
    GTTCCCGAGA AGAAAGAGGC TAAAGTCATT GAAAAGAAAG TTATACCAGC TCCCGTAAAG
    AAAGAGCCTG AAGTTGTTAA GGAAAAGGCT CCTGAACTGA CAACAAAAAA AGCACCTGAG
    ATAACCAAAG AAAAACCGAA GCCAGTACCA AAGAAAGAGG CTGAAGTCAT TAAAGAGAAA
    GCTAAACTAG CTCCTGAAAA GAAAGAGGTT GAAGTCATCA AGGAGAAAGT TAAACCAGCT
    CCGTTAAAGA AAGAGCCTGA GGTTCATAAG GAAAAAGCAC CTGAACCAAA ATCAGAACCT
    ACAAAAAAAG TTGAGGTTCC AAAGGAAAAG GCAAAACCAG TTGCTAAAGA AAAGGAACCT
    GAAATAACCA AAGAAAAGCC AAAACCAGCA CCAAAGAAAG AAGCTGTAGT CATCAAAGAA
    AAAGCTGTAC CAGCCATCCT GAAGGAAGTA AAGAAAGTTA AAGCAGCTCC TGAGAAGAAA
    GTGGCTGAAA TTAAGGAGAA AGTTAAACCA GCTTCTATAA TGAAAGAGCC TGAAGTTGTG
    AAACAAAAGA CTCCTGAACC GAAACCCAAA AAAGAGCCTG AAGTTGTGAA AGAAAAGGCT
    CCTGAACCTA AACCCAAAAA AGAGCCTGAA GTTGCTAAAG AGAAAGCCAA ACCAGCACCT
    CAGAAGAAAG AAGTCATCAA AGAAAAAGTC AAACTTGCTC CTGTAAAGAA AGTGCCCGAA
    GTTGTCGAAG AAAAGGCACT TAAACCAGAA CCCAAAAAAG ATGAAGTTGG TCTTATTGGT
    CTGGACCTGG AGCTCCTAAA CTCCATCAAC CAAAAACTGT CTCTCCTGGA CTTGCTAAGG
    AAGGACATTG GAGAAATGAA AAGCGATCTG GAGTCCACTC AGAATCAAAT CACACTTTTA
    CGGATGGACA ACAGAACGAT CAAAGAGCCA GAGACTATTG GTGTGAAAGT AAAACCAGCA
    GTCAAGGAAA AAGCAAAAAG AGACCACGCT CTGATCAATG CTACTAGGAC TGCACATATT
    AAAACAGAGC GTGAATCTCT AAAAAACATC ACGAAACCAG TGCCTGCAAA GAAAGCCAAA
    GAGGCTCCAA AGGTTAAGAT TGCGCTGGAG CCTAAAAAGA AAGGAATCAT CCCTGAGAAG
    GTCAAAGAAG TAAAGGAAAC TTTACAGAAA GAACCTGAGT TGGTCAAAGA TAAAGTAGTT
    GAGCCAATAG AGAAGGAAAT TGTTGTTGAG AAGGAAGTCA AAGGAGAAGA GCCTGAAATC
    ACCAAAGATA TTCCAGAGGT GGAGGAAGAG GACATTCCCT ACTTCCAGTG TTTCTTTGTG
    GATGAGGATG ACACTCAGTA TCCGTTCTTC CCTTTCTCTC CACCGCTTTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55701
    Clone ID Related Accession (Same CDS sequence) XM_017351783.1
    Accession Version XM_017351783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGAAACCAAA AGCAGTTCCC
    GAGAAGAAAG AGGCTAAAGT CATTGAAAAG AAAGTTATAC CAGCTCCCGT AAAGAAAGAG
    CCTGAAGTTG TTAAGGAAAA GGCTCCTGAA CTGACAACAA AAAAAGCACC TGAGATAACC
    AAAGAAAAAC CGAAGCCAGT ACCAAAGAAA GAGGCTGAAG TCATTAAAGA GAAAGCTAAA
    CTAGCTCCTG AAAAGAAAGA GGTTGAAGTC ATCAAGGAGA AAGTTAAACC AGCTCCGTTA
    AAGAAAGAGC CTGAGGTTCA TAAGGAAAAA GCACCTGAAC CAAAATCAGA ACCTACAAAA
    AAAGTTGAGG TTCCAAAGGA AAAGGCAAAA CCAGTTGCTA AAGAAAAGGA ACCTGAAATA
    ACCAAAGAAA AGCCAAAACC AGCACCAAAG AAAGAAGCTG TAGTCATCAA AGAAAAAGCT
    GTACCAGCCA TCCTGAAGGA AGTAAAGAAA GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT
    GAAATTAAGG AGAAAGTTAA ACCAGCTTCT ATAATGAAAG AGCCTGAAGT TGTGAAACAA
    AAGACTCCTG AACCGAAACC CAAAAAAGAG CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA
    CCTAAACCCA AAAAAGAGCC TGAAGTTGCT AAAGAGAAAG CCAAACCAGC ACCTCAGAAG
    AAAGAAGTCA TCAAAGAAAA AGTCAAACTT GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC
    GAAGAAAAGG CACTTAAACC AGAACCCAAA AAAGATGAAG TTGGTCTTAT TGGTCTGGAC
    CTGGAGCTCC TAAACTCCAT CAACCAAAAA CTGTCTCTCC TGGACTTGCT AAGGAAGGAC
    ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC ACTCAGAATC AAATCACACT TTTACGGATG
    GACAACAGAA CGATCAAAGA GCCAGAGACT ATTGGTGTGA AAGTAAAACC AGCAGTCAAG
    GAAAAAGCAA AAAGAGACCA CGCTCTGATC AATGCTACTA GGACTGCACA TATTAAAACA
    GAGCGTGAAT CTCTAAAAAA CATCACGAAA CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT
    CCAAAGGTTA AGATTGCGCT GGAGCCTAAA AAGAAAGGAA TCATCCCTGA GAAGGTCAAA
    GAAGTAAAGG AAACTTTACA GAAAGAACCT GAGTTGGTCA AAGATAAAGT AGTTGAGCCA
    ATAGAGAAGG AAATTGTTGT TGAGAAGGAA GTCAAAGGAG AAGAGCCTGA AATCACCAAA
    GATATTCCAG AGGTGGAGGA AGAGGACATT CCCTACTTCC AGTGTTTCTT TGTGGATGAG
    GATGACACTC AGTATCCGTT CTTCCCTTTC TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207272.1
    CDS343..8130
    Translation

    Target ORF information:

    RefSeq Version XM_017351783.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAAGAGGCT GAAGCTGTAA AGGAGAAAAC GAAACCAACC
    TCTGAGAAGA AAGAGGCTGA AATAATTAAG GAGAAAGTTA AAGCAGCTCC TGTGAAGAAA
    GAAGTTGAAG TCAAGGAGGA AGCAAAACCA GCTCCTGTAA AAAAAGAAAC TGAAGTCATC
    AAAGAAAAAG AAAAAACAGC TCCTGAGAAG AAAGAGGCTG AAGCTGTAAA GGAGAAAACG
    AAACCAACCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGATAGTTAA ACAAGCTCCT
    GTGAAGAAAG AGGCTGAACC TGTAAAGGAG ATAACTAAAC CAGCCCCTGA GAAGAAAGAG
    GCTGAAATAA TCAAGGAGAA AGTTAAAGCA GCACCTCTGA AGAAAGAAGT CAAGGAGAAA
    GCAAAACCAG CTCCTGTAAA GAAAGAAGCT GAAGTCATCA AACAAAAAGC CAAACCAGCT
    CCTGAGAAGA AAGAGGCCAA AGTCATCAAG GAGAAAGTTA AACCGGCTCC TGTAAAGATA
    GAGGCTAAAC CTGTAAAGGA GATAACTAAA CCAGCCCCTG AGAAGAAAGA GGCTGAAATA
    ATCAAGGAGA AGGTCAAATC AGCTCCTGTG AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA
    AAACCAGCTC CTGAAAAGAA AGAGGCTGAA GTCATCAAGG AGAAAGTTAA ACCTGCTCCT
    GTGAGGAAAG AAGCTGAAGA AATAAAGGAA AAAGCTGAAG CAGTTTATGA GAAAAAAGAG
    GCTGAAGTAG CCAAGGAGAT AGTTAAACCA ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG
    AAGGAAAAGG CTCCTGAATC AAAACCCCAA AAAGAGGCTG AAATTATCAA AGAGAAAGTT
    AAACCTGCAC CTGTAAAGAA AGAGCCTGAA GTTGCAAAAG TACCAAAACC CAAAAAAGAG
    GCTGAACTCG CCAAAGAGAA AGTTAAACCT GCACCTGTAA AGAAAGAAGA AAAACCTAAG
    GCAGCACCAA AGAAAGAGGC TGAAGCTATT AAGGAGAAAC GTACACCAGC TCCTGAAAAG
    AAAGTGGTAG AAGTCATCAA GGAGAAAGTC AAACCAGCCC CTGAGAAAAA AGCGGTTCTC
    AAGGAGAAAG TTAGACCTGC TCCTGAAAAG AAAGAGGTTG AAGTCATCAA AGAGAAAGCT
    AAACCAGCCC TTAAGAAGAA AGAGCCTAAA GTCAAGGAAA AGGTTCAACC AGCTCCTATA
    AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG GTGCCTGAAC CAACACCAGA ACCTGAGAGA
    GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA ATCCCTAGGG AAAAAGTAGC TGAGATTACT
    AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA GAGGCTGAAG TAAAGGAGAA AGCTACACCT
    GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC AAGGAGAAAG TTAAACAAGC TCCTGTAAAG
    AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT CCTGAACCAA AACCTAAAAA GACTGAAGTC
    ATCAAAGCGA AAGTAAAACC AGCCCCTGAG AAAAAAGAGG CTGAAGTGAA GGAGAAAATT
    AAACCAGTCC CTGAGAAGAA AGAGGCTGAA GTCATCAAGG AGAAACCAAA AGCAGTTCCC
    GAGAAGAAAG AGGCTAAAGT CATTGAAAAG AAAGTTATAC CAGCTCCCGT AAAGAAAGAG
    CCTGAAGTTG TTAAGGAAAA GGCTCCTGAA CTGACAACAA AAAAAGCACC TGAGATAACC
    AAAGAAAAAC CGAAGCCAGT ACCAAAGAAA GAGGCTGAAG TCATTAAAGA GAAAGCTAAA
    CTAGCTCCTG AAAAGAAAGA GGTTGAAGTC ATCAAGGAGA AAGTTAAACC AGCTCCGTTA
    AAGAAAGAGC CTGAGGTTCA TAAGGAAAAA GCACCTGAAC CAAAATCAGA ACCTACAAAA
    AAAGTTGAGG TTCCAAAGGA AAAGGCAAAA CCAGTTGCTA AAGAAAAGGA ACCTGAAATA
    ACCAAAGAAA AGCCAAAACC AGCACCAAAG AAAGAAGCTG TAGTCATCAA AGAAAAAGCT
    GTACCAGCCA TCCTGAAGGA AGTAAAGAAA GTTAAAGCAG CTCCTGAGAA GAAAGTGGCT
    GAAATTAAGG AGAAAGTTAA ACCAGCTTCT ATAATGAAAG AGCCTGAAGT TGTGAAACAA
    AAGACTCCTG AACCGAAACC CAAAAAAGAG CCTGAAGTTG TGAAAGAAAA GGCTCCTGAA
    CCTAAACCCA AAAAAGAGCC TGAAGTTGCT AAAGAGAAAG CCAAACCAGC ACCTCAGAAG
    AAAGAAGTCA TCAAAGAAAA AGTCAAACTT GCTCCTGTAA AGAAAGTGCC CGAAGTTGTC
    GAAGAAAAGG CACTTAAACC AGAACCCAAA AAAGATGAAG TTGGTCTTAT TGGTCTGGAC
    CTGGAGCTCC TAAACTCCAT CAACCAAAAA CTGTCTCTCC TGGACTTGCT AAGGAAGGAC
    ATTGGAGAAA TGAAAAGCGA TCTGGAGTCC ACTCAGAATC AAATCACACT TTTACGGATG
    GACAACAGAA CGATCAAAGA GCCAGAGACT ATTGGTGTGA AAGTAAAACC AGCAGTCAAG
    GAAAAAGCAA AAAGAGACCA CGCTCTGATC AATGCTACTA GGACTGCACA TATTAAAACA
    GAGCGTGAAT CTCTAAAAAA CATCACGAAA CCAGTGCCTG CAAAGAAAGC CAAAGAGGCT
    CCAAAGGTTA AGATTGCGCT GGAGCCTAAA AAGAAAGGAA TCATCCCTGA GAAGGTCAAA
    GAAGTAAAGG AAACTTTACA GAAAGAACCT GAGTTGGTCA AAGATAAAGT AGTTGAGCCA
    ATAGAGAAGG AAATTGTTGT TGAGAAGGAA GTCAAAGGAG AAGAGCCTGA AATCACCAAA
    GATATTCCAG AGGTGGAGGA AGAGGACATT CCCTACTTCC AGTGTTTCTT TGTGGATGAG
    GATGACACTC AGTATCCGTT CTTCCCTTTC TCTCCACCGC TTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55702
    Clone ID Related Accession (Same CDS sequence) XM_017351784.1
    Accession Version XM_017351784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAGGCT GAAGCCGTAA AGGAGAAAAC GAAACCAGCC
    CCTGAGAAAG AGACTGAAAT AATCAAGGAT AAAATTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGAAGGAAGC AAAACCAGCT CCTGTAAAGA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AACCAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCTCTG AGAAGAAAGA GGCTGAAATA ATTAAGGAGA AAGTTAAAGC AGCTCCTGTG
    AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA AAACCAGCTC CTGTAAAAAA AGAAACTGAA
    GTCATCAAAG AAAAAGAAAA AACAGCTCCT GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG
    AAAACGAAAC CAACCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAT AGTTAAACAA
    GCTCCTGTGA AGAAAGAGGC TGAACCTGTA AAGGAGATAA CTAAACCAGC CCCTGAGAAG
    AAAGAGGCTG AAATAATCAA GGAGAAAGTT AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG
    GAGAAAGCAA AACCAGCTCC TGTAAAGAAA GAAGCTGAAG TCATCAAACA AAAAGCCAAA
    CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC ATCAAGGAGA AAGTTAAACC GGCTCCTGTA
    AAGATAGAGG CTAAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAGGT CAAATCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG
    GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG GCTGAAGTCA TCAAGGAGAA AGTTAAACCT
    GCTCCTGTGA GGAAAGAAGC TGAAGAAATA AAGGAAAAAG CTGAAGCAGT TTATGAGAAA
    AAAGAGGCTG AAGTAGCCAA GGAGATAGTT AAACCAACTC CTTTAAAGAA GGAGCCCGAA
    GTTGTGAAGG AAAAGGCTCC TGAATCAAAA CCCCAAAAAG AGGCTGAAAT TATCAAAGAG
    AAAGTTAAAC CTGCACCTGT AAAGAAAGAG CCTGAAGTTG CAAAAGTACC AAAACCCAAA
    AAAGAGGCTG AACTCGCCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAAGAAAAA
    CCTAAGGCAG CACCAAAGAA AGAGGCTGAA GCTATTAAGG AGAAACGTAC ACCAGCTCCT
    GAAAAGAAAG TGGTAGAAGT CATCAAGGAG AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG
    GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT GAAAAGAAAG AGGTTGAAGT CATCAAAGAG
    AAAGCTAAAC CAGCCCTTAA GAAGAAAGAG CCTAAAGTCA AGGAAAAGGT TCAACCAGCT
    CCTATAAAGA AAGAGCCAGA GCTTCCCAAA GAAAAGGTGC CTGAACCAAC ACCAGAACCT
    GAGAGAGTTG AAGCTCCAAA AGAAAAGGCA AAAGTAATCC CTAGGGAAAA AGTAGCTGAG
    ATTACTAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGTAAA GGAGAAAGCT
    ACACCTGCTC CTGTGCACAA AGAGGTTGAA GTGTTCAAGG AGAAAGTTAA ACAAGCTCCT
    GTAAAGAAAG AGCCAGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC TAAAAAGACT
    GAAGTCATCA AAGCGAAAGT AAAACCAGCC CCTGAGAAAA AAGAGGCTGA AGTGAAGGAG
    AAAATTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TAAAGGAGAG AATTAAACCT
    GCTCCTGTAA AGAAAGAGCC TGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCCAAA
    AAAGAACCTG AAGTTATCAA AGAGAAAGTG AAACCAGCGC CAGAGAAGAA AGCACCCGAA
    ATAACCAAAG AAAAACCAAA GCCAGCAGCA AAGAAAGTAG CTGAAGTCAT CAAAGACAGA
    ACTAAACCAG TCCCTGAGAA GAAAGAGGCT GAAGTCATCA AGGAGAAACC AAAAGCAGTT
    CCCGAGAAGA AAGAGGCTAA AGTCATTGAA AAGAAAGTTA TACCAGCTCC CGTAAAGAAA
    GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT GAACTGACAA CAAAAAAAGC ACCTGAGATA
    ACCAAAGAAA AACCGAAGCC AGTACCAAAG AAAGAGGCTG AAGTCATTAA AGAGAAAGCT
    AAACTAGCTC CTGAAAAGAA AGAGGTTGAA GTCATCAAGG AGAAAGTTAA ACCAGCTCCG
    TTAAAGAAAG AGCCTGAGGT TCATAAGGAA AAAGCACCTG AACCAAAATC AGAACCTACA
    AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA AAACCAGTTG CTAAAGAAAA GGAACCTGAA
    ATAACCAAAG AAAAGCCAAA ACCAGCACCA AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA
    GCTGTACCAG CCATCCTGAA GGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207273.1
    CDS342..8012
    Translation

    Target ORF information:

    RefSeq Version XM_017351784.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAGGCT GAAGCCGTAA AGGAGAAAAC GAAACCAGCC
    CCTGAGAAAG AGACTGAAAT AATCAAGGAT AAAATTAAAG CAGCTCCTGT GAAGAAAGAA
    GTTGAAGTCA AGAAGGAAGC AAAACCAGCT CCTGTAAAGA AAGAAACTGA AGTCATCAAA
    GAAAAAGAAA AACCAGCTCC TGAGAAGAAA GAGGCTGAAG CTGTAAAGGA GAAAACGAAA
    CCAACCTCTG AGAAGAAAGA GGCTGAAATA ATTAAGGAGA AAGTTAAAGC AGCTCCTGTG
    AAGAAAGAAG TTGAAGTCAA GGAGGAAGCA AAACCAGCTC CTGTAAAAAA AGAAACTGAA
    GTCATCAAAG AAAAAGAAAA AACAGCTCCT GAGAAGAAAG AGGCTGAAGC TGTAAAGGAG
    AAAACGAAAC CAACCCCTGA GAAGAAAGAG GCTGAAGTCA TCAAGGAGAT AGTTAAACAA
    GCTCCTGTGA AGAAAGAGGC TGAACCTGTA AAGGAGATAA CTAAACCAGC CCCTGAGAAG
    AAAGAGGCTG AAATAATCAA GGAGAAAGTT AAAGCAGCAC CTCTGAAGAA AGAAGTCAAG
    GAGAAAGCAA AACCAGCTCC TGTAAAGAAA GAAGCTGAAG TCATCAAACA AAAAGCCAAA
    CCAGCTCCTG AGAAGAAAGA GGCCAAAGTC ATCAAGGAGA AAGTTAAACC GGCTCCTGTA
    AAGATAGAGG CTAAACCTGT AAAGGAGATA ACTAAACCAG CCCCTGAGAA GAAAGAGGCT
    GAAATAATCA AGGAGAAGGT CAAATCAGCT CCTGTGAAGA AAGAAGTTGA AGTCAAGGAG
    GAAGCAAAAC CAGCTCCTGA AAAGAAAGAG GCTGAAGTCA TCAAGGAGAA AGTTAAACCT
    GCTCCTGTGA GGAAAGAAGC TGAAGAAATA AAGGAAAAAG CTGAAGCAGT TTATGAGAAA
    AAAGAGGCTG AAGTAGCCAA GGAGATAGTT AAACCAACTC CTTTAAAGAA GGAGCCCGAA
    GTTGTGAAGG AAAAGGCTCC TGAATCAAAA CCCCAAAAAG AGGCTGAAAT TATCAAAGAG
    AAAGTTAAAC CTGCACCTGT AAAGAAAGAG CCTGAAGTTG CAAAAGTACC AAAACCCAAA
    AAAGAGGCTG AACTCGCCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAAGAAAAA
    CCTAAGGCAG CACCAAAGAA AGAGGCTGAA GCTATTAAGG AGAAACGTAC ACCAGCTCCT
    GAAAAGAAAG TGGTAGAAGT CATCAAGGAG AAAGTCAAAC CAGCCCCTGA GAAAAAAGCG
    GTTCTCAAGG AGAAAGTTAG ACCTGCTCCT GAAAAGAAAG AGGTTGAAGT CATCAAAGAG
    AAAGCTAAAC CAGCCCTTAA GAAGAAAGAG CCTAAAGTCA AGGAAAAGGT TCAACCAGCT
    CCTATAAAGA AAGAGCCAGA GCTTCCCAAA GAAAAGGTGC CTGAACCAAC ACCAGAACCT
    GAGAGAGTTG AAGCTCCAAA AGAAAAGGCA AAAGTAATCC CTAGGGAAAA AGTAGCTGAG
    ATTACTAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGTAAA GGAGAAAGCT
    ACACCTGCTC CTGTGCACAA AGAGGTTGAA GTGTTCAAGG AGAAAGTTAA ACAAGCTCCT
    GTAAAGAAAG AGCCAGAAGT TCCTAAAGAA AAGGCTCCTG AACCAAAACC TAAAAAGACT
    GAAGTCATCA AAGCGAAAGT AAAACCAGCC CCTGAGAAAA AAGAGGCTGA AGTGAAGGAG
    AAAATTAAAC CAGTCCCTGA GAAGAAAGAG GCTGAAGTCA TAAAGGAGAG AATTAAACCT
    GCTCCTGTAA AGAAAGAGCC TGAAGTTCCT AAAGAAAAGG CTCCTGAACC AAAACCCAAA
    AAAGAACCTG AAGTTATCAA AGAGAAAGTG AAACCAGCGC CAGAGAAGAA AGCACCCGAA
    ATAACCAAAG AAAAACCAAA GCCAGCAGCA AAGAAAGTAG CTGAAGTCAT CAAAGACAGA
    ACTAAACCAG TCCCTGAGAA GAAAGAGGCT GAAGTCATCA AGGAGAAACC AAAAGCAGTT
    CCCGAGAAGA AAGAGGCTAA AGTCATTGAA AAGAAAGTTA TACCAGCTCC CGTAAAGAAA
    GAGCCTGAAG TTGTTAAGGA AAAGGCTCCT GAACTGACAA CAAAAAAAGC ACCTGAGATA
    ACCAAAGAAA AACCGAAGCC AGTACCAAAG AAAGAGGCTG AAGTCATTAA AGAGAAAGCT
    AAACTAGCTC CTGAAAAGAA AGAGGTTGAA GTCATCAAGG AGAAAGTTAA ACCAGCTCCG
    TTAAAGAAAG AGCCTGAGGT TCATAAGGAA AAAGCACCTG AACCAAAATC AGAACCTACA
    AAAAAAGTTG AGGTTCCAAA GGAAAAGGCA AAACCAGTTG CTAAAGAAAA GGAACCTGAA
    ATAACCAAAG AAAAGCCAAA ACCAGCACCA AAGAAAGAAG CTGTAGTCAT CAAAGAAAAA
    GCTGTACCAG CCATCCTGAA GGAAGTAAAG AAAGTTAAAG CAGCTCCTGA GAAGAAAGTG
    GCTGAAATTA AGGAGAAAGT TAAACCAGCT TCTATAATGA AAGAGCCTGA AGTTGTGAAA
    CAAAAGACTC CTGAACCGAA ACCCAAAAAA GAGCCTGAAG TTGTGAAAGA AAAGGCTCCT
    GAACCTAAAC CCAAAAAAGA GCCTGAAGTT GCTAAAGAGA AAGCCAAACC AGCACCTCAG
    AAGAAAGAAG TCATCAAAGA AAAAGTCAAA CTTGCTCCTG TAAAGAAAGT GCCCGAAGTT
    GTCGAAGAAA AGGCACTTAA ACCAGAACCC AAAAAAGATG AAGTTGGTCT TATTGGTCTG
    GACCTGGAGC TCCTAAACTC CATCAACCAA AAACTGTCTC TCCTGGACTT GCTAAGGAAG
    GACATTGGAG AAATGAAAAG CGATCTGGAG TCCACTCAGA ATCAAATCAC ACTTTTACGG
    ATGGACAACA GAACGATCAA AGAGCCAGAG ACTATTGGTG TGAAAGTAAA ACCAGCAGTC
    AAGGAAAAAG CAAAAAGAGA CCACGCTCTG ATCAATGCTA CTAGGACTGC ACATATTAAA
    ACAGAGCGTG AATCTCTAAA AAACATCACG AAACCAGTGC CTGCAAAGAA AGCCAAAGAG
    GCTCCAAAGG TTAAGATTGC GCTGGAGCCT AAAAAGAAAG GAATCATCCC TGAGAAGGTC
    AAAGAAGTAA AGGAAACTTT ACAGAAAGAA CCTGAGTTGG TCAAAGATAA AGTAGTTGAG
    CCAATAGAGA AGGAAATTGT TGTTGAGAAG GAAGTCAAAG GAGAAGAGCC TGAAATCACC
    AAAGATATTC CAGAGGTGGA GGAAGAGGAC ATTCCCTACT TCCAGTGTTT CTTTGTGGAT
    GAGGATGACA CTCAGTATCC GTTCTTCCCT TTCTCTCCAC CGCTTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55703
    Clone ID Related Accession (Same CDS sequence) XM_017351785.1
    Accession Version XM_017351785.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGAGGCTGAA
    GTCATCAAGG AGAAAGTTAA ACCTGCTCCT GTGAGGAAAG AAGCTGAAGA AATAAAGGAA
    AAAGCTGAAG CAGTTTATGA GAAAAAAGAG GCTGAAGTAG CCAAGGAGAT AGTTAAACCA
    ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG AAGGAAAAGG CTCCTGAATC AAAACCCCAA
    AAAGAGGCTG AAATTATCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAGCCTGAA
    GTTGCAAAAG TACCAAAACC CAAAAAAGAG GCTGAACTCG CCAAAGAGAA AGTTAAACCT
    GCACCTGTAA AGAAAGAAGA AAAACCTAAG GCAGCACCAA AGAAAGAGGC TGAAGCTATT
    AAGGAGAAAC GTACACCAGC TCCTGAAAAG AAAGTGGTAG AAGTCATCAA GGAGAAAGTC
    AAACCAGCCC CTGAGAAAAA AGCGGTTCTC AAGGAGAAAG TTAGACCTGC TCCTGAAAAG
    AAAGAGGTTG AAGTCATCAA AGAGAAAGCT AAACCAGCCC TTAAGAAGAA AGAGCCTAAA
    GTCAAGGAAA AGGTTCAACC AGCTCCTATA AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG
    GTGCCTGAAC CAACACCAGA ACCTGAGAGA GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA
    ATCCCTAGGG AAAAAGTAGC TGAGATTACT AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA
    GAGGCTGAAG TAAAGGAGAA AGCTACACCT GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC
    AAGGAGAAAG TTAAACAAGC TCCTGTAAAG AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT
    CCTGAACCAA AACCTAAAAA GACTGAAGTC ATCAAAGCGA AAGTAAAACC AGCCCCTGAG
    AAAAAAGAGG CTGAAGTGAA GGAGAAAATT AAACCAGTCC CTGAGAAGAA AGAGGCTGAA
    GTCATAAAGG AGAGAATTAA ACCTGCTCCT GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA
    AAGGCTCCTG AACCAAAACC CAAAAAAGAA CCTGAAGTTA TCAAAGAGAA AGTGAAACCA
    GCGCCAGAGA AGAAAGCACC CGAAATAACC AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA
    GTAGCTGAAG TCATCAAAGA CAGAACTAAA CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA AACCAAAAGC AGTTCCCGAG AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA
    GTTATACCAG CTCCCGTAAA GAAAGAGCCT GAAGTTGTTA AGGAAAAGGC TCCTGAACTG
    ACAACAAAAA AAGCACCTGA GATAACCAAA GAAAAACCGA AGCCAGTACC AAAGAAAGAG
    GCTGAAGTCA TTAAAGAGAA AGCTAAACTA GCTCCTGAAA AGAAAGAGGT TGAAGTCATC
    AAGGAGAAAG TTAAACCAGC TCCGTTAAAG AAAGAGCCTG AGGTTCATAA GGAAAAAGCA
    CCTGAACCAA AATCAGAACC TACAAAAAAA GTTGAGGTTC CAAAGGAAAA GGCAAAACCA
    GTTGCTAAAG AAAAGGAACC TGAAATAACC AAAGAAAAGC CAAAACCAGC ACCAAAGAAA
    GAAGCTGTAG TCATCAAAGA AAAAGCTGTA CCAGCCATCC TGAAGGAAGT AAAGAAAGTT
    AAAGCAGCTC CTGAGAAGAA AGTGGCTGAA ATTAAGGAGA AAGTTAAACC AGCTTCTATA
    ATGAAAGAGC CTGAAGTTGT GAAACAAAAG ACTCCTGAAC CGAAACCCAA AAAAGAGCCT
    GAAGTTGTGA AAGAAAAGGC TCCTGAACCT AAACCCAAAA AAGAGCCTGA AGTTGCTAAA
    GAGAAAGCCA AACCAGCACC TCAGAAGAAA GAAGTCATCA AAGAAAAAGT CAAACTTGCT
    CCTGTAAAGA AAGTGCCCGA AGTTGTCGAA GAAAAGGCAC TTAAACCAGA ACCCAAAAAA
    GATGAAGTTG GTCTTATTGG TCTGGACCTG GAGCTCCTAA ACTCCATCAA CCAAAAACTG
    TCTCTCCTGG ACTTGCTAAG GAAGGACATT GGAGAAATGA AAAGCGATCT GGAGTCCACT
    CAGAATCAAA TCACACTTTT ACGGATGGAC AACAGAACGA TCAAAGAGCC AGAGACTATT
    GGTGTGAAAG TAAAACCAGC AGTCAAGGAA AAAGCAAAAA GAGACCACGC TCTGATCAAT
    GCTACTAGGA CTGCACATAT TAAAACAGAG CGTGAATCTC TAAAAAACAT CACGAAACCA
    GTGCCTGCAA AGAAAGCCAA AGAGGCTCCA AAGGTTAAGA TTGCGCTGGA GCCTAAAAAG
    AAAGGAATCA TCCCTGAGAA GGTCAAAGAA GTAAAGGAAA CTTTACAGAA AGAACCTGAG
    TTGGTCAAAG ATAAAGTAGT TGAGCCAATA GAGAAGGAAA TTGTTGTTGA GAAGGAAGTC
    AAAGGAGAAG AGCCTGAAAT CACCAAAGAT ATTCCAGAGG TGGAGGAAGA GGACATTCCC
    TACTTCCAGT GTTTCTTTGT GGATGAGGAT GACACTCAGT ATCCGTTCTT CCCTTTCTCT
    CCACCGCTTT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207274.1
    CDS342..7436
    Translation

    Target ORF information:

    RefSeq Version XM_017351785.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGAGGCTGAA
    GTCATCAAGG AGAAAGTTAA ACCTGCTCCT GTGAGGAAAG AAGCTGAAGA AATAAAGGAA
    AAAGCTGAAG CAGTTTATGA GAAAAAAGAG GCTGAAGTAG CCAAGGAGAT AGTTAAACCA
    ACTCCTTTAA AGAAGGAGCC CGAAGTTGTG AAGGAAAAGG CTCCTGAATC AAAACCCCAA
    AAAGAGGCTG AAATTATCAA AGAGAAAGTT AAACCTGCAC CTGTAAAGAA AGAGCCTGAA
    GTTGCAAAAG TACCAAAACC CAAAAAAGAG GCTGAACTCG CCAAAGAGAA AGTTAAACCT
    GCACCTGTAA AGAAAGAAGA AAAACCTAAG GCAGCACCAA AGAAAGAGGC TGAAGCTATT
    AAGGAGAAAC GTACACCAGC TCCTGAAAAG AAAGTGGTAG AAGTCATCAA GGAGAAAGTC
    AAACCAGCCC CTGAGAAAAA AGCGGTTCTC AAGGAGAAAG TTAGACCTGC TCCTGAAAAG
    AAAGAGGTTG AAGTCATCAA AGAGAAAGCT AAACCAGCCC TTAAGAAGAA AGAGCCTAAA
    GTCAAGGAAA AGGTTCAACC AGCTCCTATA AAGAAAGAGC CAGAGCTTCC CAAAGAAAAG
    GTGCCTGAAC CAACACCAGA ACCTGAGAGA GTTGAAGCTC CAAAAGAAAA GGCAAAAGTA
    ATCCCTAGGG AAAAAGTAGC TGAGATTACT AAAGAAAAAC CAAAGCCAGC ACCAAAGAAA
    GAGGCTGAAG TAAAGGAGAA AGCTACACCT GCTCCTGTGC ACAAAGAGGT TGAAGTGTTC
    AAGGAGAAAG TTAAACAAGC TCCTGTAAAG AAAGAGCCAG AAGTTCCTAA AGAAAAGGCT
    CCTGAACCAA AACCTAAAAA GACTGAAGTC ATCAAAGCGA AAGTAAAACC AGCCCCTGAG
    AAAAAAGAGG CTGAAGTGAA GGAGAAAATT AAACCAGTCC CTGAGAAGAA AGAGGCTGAA
    GTCATAAAGG AGAGAATTAA ACCTGCTCCT GTAAAGAAAG AGCCTGAAGT TCCTAAAGAA
    AAGGCTCCTG AACCAAAACC CAAAAAAGAA CCTGAAGTTA TCAAAGAGAA AGTGAAACCA
    GCGCCAGAGA AGAAAGCACC CGAAATAACC AAAGAAAAAC CAAAGCCAGC AGCAAAGAAA
    GTAGCTGAAG TCATCAAAGA CAGAACTAAA CCAGTCCCTG AGAAGAAAGA GGCTGAAGTC
    ATCAAGGAGA AACCAAAAGC AGTTCCCGAG AAGAAAGAGG CTAAAGTCAT TGAAAAGAAA
    GTTATACCAG CTCCCGTAAA GAAAGAGCCT GAAGTTGTTA AGGAAAAGGC TCCTGAACTG
    ACAACAAAAA AAGCACCTGA GATAACCAAA GAAAAACCGA AGCCAGTACC AAAGAAAGAG
    GCTGAAGTCA TTAAAGAGAA AGCTAAACTA GCTCCTGAAA AGAAAGAGGT TGAAGTCATC
    AAGGAGAAAG TTAAACCAGC TCCGTTAAAG AAAGAGCCTG AGGTTCATAA GGAAAAAGCA
    CCTGAACCAA AATCAGAACC TACAAAAAAA GTTGAGGTTC CAAAGGAAAA GGCAAAACCA
    GTTGCTAAAG AAAAGGAACC TGAAATAACC AAAGAAAAGC CAAAACCAGC ACCAAAGAAA
    GAAGCTGTAG TCATCAAAGA AAAAGCTGTA CCAGCCATCC TGAAGGAAGT AAAGAAAGTT
    AAAGCAGCTC CTGAGAAGAA AGTGGCTGAA ATTAAGGAGA AAGTTAAACC AGCTTCTATA
    ATGAAAGAGC CTGAAGTTGT GAAACAAAAG ACTCCTGAAC CGAAACCCAA AAAAGAGCCT
    GAAGTTGTGA AAGAAAAGGC TCCTGAACCT AAACCCAAAA AAGAGCCTGA AGTTGCTAAA
    GAGAAAGCCA AACCAGCACC TCAGAAGAAA GAAGTCATCA AAGAAAAAGT CAAACTTGCT
    CCTGTAAAGA AAGTGCCCGA AGTTGTCGAA GAAAAGGCAC TTAAACCAGA ACCCAAAAAA
    GATGAAGTTG GTCTTATTGG TCTGGACCTG GAGCTCCTAA ACTCCATCAA CCAAAAACTG
    TCTCTCCTGG ACTTGCTAAG GAAGGACATT GGAGAAATGA AAAGCGATCT GGAGTCCACT
    CAGAATCAAA TCACACTTTT ACGGATGGAC AACAGAACGA TCAAAGAGCC AGAGACTATT
    GGTGTGAAAG TAAAACCAGC AGTCAAGGAA AAAGCAAAAA GAGACCACGC TCTGATCAAT
    GCTACTAGGA CTGCACATAT TAAAACAGAG CGTGAATCTC TAAAAAACAT CACGAAACCA
    GTGCCTGCAA AGAAAGCCAA AGAGGCTCCA AAGGTTAAGA TTGCGCTGGA GCCTAAAAAG
    AAAGGAATCA TCCCTGAGAA GGTCAAAGAA GTAAAGGAAA CTTTACAGAA AGAACCTGAG
    TTGGTCAAAG ATAAAGTAGT TGAGCCAATA GAGAAGGAAA TTGTTGTTGA GAAGGAAGTC
    AAAGGAGAAG AGCCTGAAAT CACCAAAGAT ATTCCAGAGG TGGAGGAAGA GGACATTCCC
    TACTTCCAGT GTTTCTTTGT GGATGAGGAT GACACTCAGT ATCCGTTCTT CCCTTTCTCT
    CCACCGCTTT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55704
    Clone ID Related Accession (Same CDS sequence) XM_017351786.1
    Accession Version XM_017351786.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207275.1
    CDS339..5105
    Translation

    Target ORF information:

    RefSeq Version XM_017351786.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAGTTGAA
    GTCAAGAAGG AAGCAAAACC AGCTCCTGTA AAGAAAGAAA CTGAAGTCAT CAAAGAAAAA
    GAAAAACCAG CTCCTGAGAA GAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55705
    Clone ID Related Accession (Same CDS sequence) XM_017351787.1
    Accession Version XM_017351787.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product titin isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAACTGAA
    GTCATCAAAG AAAAAGAAAA ACCAGCTCCT GAGAAGAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207276.1
    CDS339..5060
    Translation

    Target ORF information:

    RefSeq Version XM_017351787.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-266g18.10 (si:ch211-266g18.10), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTCAGG CAACCAAAGT AGTGAAATCC ATCCCTCCCA CCGCTGTAGA TGCTCCGGCT 
    AGAGCAGTGC ATCCAGAATC CTTTACAGCC AGGAGTCATG GAATTTTACT AACCATGAGT
    CGTCCAGCAC AATGGGTGGT CATCATCACA CTTCTGATCT CTTGGTCAAT AGCTGGTGTT
    GTCATGTTTG ACTTTGTCAG TGATGATCAG ATTGCAAGCA TCCAGGATTT AGGTTCAGAT
    CCAGCGCTAG CAGTGAACAT GGCCATTGAA GGAATCGAAA ACAGGATAAA CCACATGAAT
    AATATGTTTA ATGATGCTAA TGAATACATT GGTGAATTAT TATATAATCC GAAGGAAGCT
    CTTTATTCAG CAGCTGACTC ATCCGTGTCT TATGTGACAG AAATGCTCAC ACCTGATGAA
    TTAATCATCG AAACTGTGAA ATATGGTGGA ACTGCATTGG ATGAGGTTAC AGAATGGGTC
    AGATTTCCAG TCTGCTGCTT ATTCCACTTA TACGAAGACG TCCTGGATAT AGTCTTTACG
    CCAGTGGATT TGCTGAGTGA AGCTGTTGTG CAAATTTTAA GTGGAATCAA GACTATTGTA
    CAGTATCTTT CAAACATATT GGAAGGCGCT AGAGACATCA CTGATATTGC CAAACTTGGA
    GGCACTGTAT TGGATAAGGT TACTGACTGT ATTCGATTAC CAGTGGGTTA CTTATTTTAC
    TTATTTGAAG CCATCCTGGA GGTAACCATT ATTTATCCAG CGGATTTGAC AAGCGAAGTA
    TTTCTGCAAA TTTTAAATGT GATCAAGAAC ACTTTATCAG CCATATTTGG GTTCTTCAAA
    GGGGATGCAG ACATCACTGA AACAGCCAAA CTTGGAGGCA CTGTATTGGA TACAGTTACT
    GACTGGATTC GATTGCCAGT GGGTTACTTA TTTTACTTGT TTGAAGCCAT TCTGGAGGTA
    ACCATTATTT ATCCAGCGGA TTTGGCAAGT GAAGCATTCC TACAAATTTT AAATGTGATC
    AAGAACACTT TATCAGCCAT ATTTGGGTTC CTCAAAGGGG ATGCAGACAT CACTGAAACA
    GCCAAACTTG GAGGCACTGT ATTGGATACA GTTACTGACT GGATTCGATT GCCAGTGGGA
    TACTTATTTT ATTTATTTGA AGCCATCCTG GAGGTAACCA TTATTTATCC AGCAGATATG
    GCAAGTGAAG CATTTTTGCA AATTTTAAAT GTGATCAAGA ACACTTCATC AGCCATATTT
    GGGTTTCTTG AAGCTTGGTT CCCCAAAATG AGCACTCGTC CTACAGAGCT GGTTGAAAAG
    GTTGCAGAGG AGGCCACTGA TGTAAAGACC ACCGTCTCTA ATTATCTGTC CAGTCTGTTT
    GTGAGGGATG AAGGTGGTAT TCCTGACGTC ACTTTTGATC CCATGAAAGT TGTCTCAGAC
    ACCGTTGAGG AGTTTGCAGA CAGAAGAGAT ATGTTATTAG CTTATCTCTC AAACATGCTC
    ATGGAAGACA AAGTTTCAGA TGAAACACCA CAGGTGATAA GGCGAAAAGG AGAATTTCTG
    CCCCCTATGG AAAAAGTTGC AGAAGTACTA GGAAAAACTC AGGACCCCAT CTACGCTGCC
    TACATCGCTG TGAGATCCAC AGCTGAGACA GACGGCGATG AACTGGAAAT AAAGGGGGCT
    GAGGTGGACT CTGCTGAAGA TATCTTGGAA GAACAAAAAG TTGAAAAGAC AGTGGACGGC
    TCTGAAAATG ATGCAGTTGC CTCTGTTGAG GAGGAAAAAG CAGTTGTTGA TATTGTAGAT
    GAGGAATCTT CTCATACGCC TGAAGTCACA GATGAAGAAA TGGCAAAAGA AAAAGATGAA
    GACACTTCTG ATTCAACTGA AATGACTTCT GATGTGCAAG ACATTGATGA TACCATAGAT
    TCTGAGACTG AAGAAATTCC TGAAATGGTT GAAAGTGTTT CAGAGGTTGG AGAAGAGGAA
    CCTTTTGTAA TTGAAAAGGT TGAAGACACC TCTGATTCGA AAGAAATCAC TTCTAATGCC
    GCAGATGTGC AAGATGTTGA TGATAATTCT GATGTTGATA CTGAAGAAAT TCCTGAATTG
    GTTGGAGGTG TTTCAGATGT TGGTGAAGAG GAACCTTCTG AAGTCATAGA TGAAGAAATT
    GCAACTGAAA AGCTTGAAGA CGCCTCTGAT TCAACTGGAT TGATTCCCAG TGCTGAAAAT
    GTGAAAGACA CTGACGATAT CTTAGAAGTT GACGCAGAGG TGAATGAAGT TGAAACAGTT
    GAAGACATTT CAGAAGTTAC AAATGAGGAA ACTTCTGATG TGCCAGAAGT CATAGATGAG
    GAAAAGGTTG AAAAGTCTGA TGATATCTTT CATTTTGCAG AGGACAAACC AACTGAATTG
    ATTTCTGATG CTCCAGATGT AACAGAAGAC ATTGAAATCT CAATCTCTGC AGTTTCAGAG
    GATAATGAAA CAATTCAAGA AGCTGATATG ACAGATGTCA AAGATGAGGA AATGGAAGCT
    GAAAAGGTTG ATGAAATCTC CTATTTTGTA GAGGATAAAT CAACTGAAAT TACTTCTGAT
    GCTACAGATG TGAAAGAAGA CACTGATGGC TTTACAGTTA CAGAGGAGAA AGGAACAATT
    GAAAGCCTTC AAGAAGCAGC AATGAAAATG TCGGGAAATG CAGATGATGA AATCCCCAAT
    GTCACAGAGA TGGTTGAAGA ACCTGATACT TCTGATGTTA TGGATGAGCT AGACGAACCT
    GAAAAAGATG AAGATGTAAT CAGTGTCACC AATGAAAGGG TTACAACTGC ACAAGATGTC
    ATATTTTCAA AGGAAAAAAT GGGAGCAGCA GAACTGGATG ATCATGACAA ACTCAGGGCT
    AAGGATGATT CAGAAGTGGA TCACATGGAG AAGGAAATTG CAGATGCTGA AAATGACCTT
    CAGAATGAGA TAGATAAAGA TGAGTTTAAG GTTGAAGATG CATCTCATCA AACTATTTTG
    GGAAGCACTG CTGCTGAAGA AGACCAAGAT GATGATGATG ATGATGAAGA ACCTGTCCTT
    CTTACTGTCA AACATGAGCT CACAGACGGT GATGATGAAA ATAATAATAA CAACGACAGA
    AAGAGAGATG CCAGAACTAA AGGTCTGATC AATAGAAAAA CACCAAAGAG CCATTCCACT
    GAAACTGATG AACAAAAGGC GCACAAGGAT CTTCATAAAG AGTCTGCTAA AGTTCTTGAG
    CAAGTGAAAG AAGCCAAAGA GAAAAAAGCT AGAGAGGAGA TATACAAAAT CATTCAAGAA
    ATGAGGGAGG AGAAGGCTGA AGGTGTGGAG GAAGAGATCG TGTTACAACA GATAAACATA
    ACAGTAGAAA AGGTCAAGAA AGAAGAAACT GTCAAAAAGG AGGAAAAGGC TAAAAGAAAA
    GAAAAGGCTC ATAAACCAAA GGATGCAAAG ATTGCCAAAG ACAAACCTAA AGCAGTCCCA
    GTAAAGCAAG AGGATGAAGT AGCCAAGAAA GAAACCAAAC CAGCACCATC TAAGAAAGCA
    GAGGCTAAAA TTGTGAAGGA GAAACCCAAA GCACATCCTG CAAAGAAAGA AAAAGCTAAA
    GATATTAAGG AAAAGGCAAA ATCTGGTCAA AAAGAAAAAG TTGCTGAGGC TGTCAAGGAA
    AAATCGAAGC CAGTAAAGAA AGAAGCTGAA AAAATTGATG CCAAAGTCAA ACCAGCAGCA
    GAAAAGAAAG TGGATGAAGC CCCCAAAGAG AAACCTAAAG TGAAGAAAGA GGCTGAAGTA
    ATCAAAAAGA TTAAACTAGA GCCTGAGAGG AAAGAAGCTA AAACTCCCAA GGCCAAAATT
    GAACCAGCTG GTTTGAAGAA AGATGCTGAA GCTGCTAAGG AGAAAGCAAA ACCTGCTCCA
    GTCAAGAAAG GGCCTGAGGT TTCCAGAGAA AAAGCCAAAC CAGAGCCCGC AAAAAAAGAG
    CTTGAAGCGG TCAAAGAGAA AGCAAAACCA GCACCAGCTA AAAAAGATGA AGTCAAAGAG
    AAAGAAAAAC CAGCTCCTGT AAAGAAAGCT GAAGAAATTA AAAAGGCAAA AGCAGCTCCT
    TCAAGGAAAG AAGCTGTAAA GGAAAAACCC AAACCTGTTT CCGAAAAGAA AGAACCTGCT
    GTTGCAGTTG AAAAAGCCAA GCCAAAATCT ACAAAGAAAG AAGCTGAAGT CGAGGAAAAA
    GTGAAACCTG CTCCTGAGAA GAAAGAAGCT GAAGTCATCA TGGTGAAAAC TAAACCAGTT
    CTTAAAAAGA AAGAGGCTGA AGTAATCAAA GAGGTTAAAC CAGCCCTTGA GAAGAAAGAG
    GCTGAAGTCA TCAAGGAGAA AGTTAAACCA GCTCCTGTAA AGAAAGAGCC TGAAGTTGTT
    AAAGAAAAGG AACCTGAACC AAAACCCAAA AGAGAGCCAG AAATTATTAA GGAGAAATCA
    GCACCACCAA AGAAAGTTGA GGTTCCAAAA CAAAAGGCTC CTGAAAAGAA AGCACCTGAA
    ATAACCAAAG AAAAACCAAA GCCAGCACCA AAGAAAGAGG CTGAAGCTGT AAAAGAGAAA
    GCTAAACCAG AGAAGAAAGA GGCTGAAGCC GTAAAGGAGA AAACGAAACC AGCCCCTGAG
    AAAGAGACTG AAATAATCAA GGATAAAATT AAAGCAGCTC CTGTGAAGAA AGAAACTGAA
    GTCATCAAAG AAAAAGAAAA ACCAGCTCCT GAGAAGAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.