pcyt1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pcyt1aa
Entrez Gene ID	550327
Full Name	phosphate cytidylyltransferase 1, choline, alpha a
Synonyms	zgc:110237
General protein information	Preferred Names phosphate cytidylyltransferase 1, choline, alpha a Names choline-phosphate cytidylyltransferase A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021480218.1	XP_021335893.1	choline-phosphate cytidylyltransferase A isoform X1
NM_001017634.1	NP_001017634.1	choline-phosphate cytidylyltransferase A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PCYT1A	NP_001098522.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002263	XP_003436073.1
Xenopus tropicalis (tropical clawed frog)	pcyt1a	XP_002934091.2
Homo sapiens (human)	PCYT1A	NP_005008.2
Mus musculus (house mouse)	Pcyt1a	NP_001156631.1
Drosophila melanogaster (fruit fly)	Cct2	NP_647622.1
Drosophila melanogaster (fruit fly)	Cct1	NP_728628.1
Canis lupus familiaris (dog)	PCYT1A	XP_535776.1
Danio rerio (zebrafish)	pcyt1aa	NP_001017634.1
Caenorhabditis elegans (roundworm)	pcyt-1	NP_001033539.1
Pan troglodytes (chimpanzee)	PCYT1A	XP_526433.2
Rattus norvegicus (Norway rat)	Pcyt1a	NP_511177.2
Gallus gallus (chicken)	PCYT1A	XP_422725.3
Caenorhabditis elegans (roundworm)	Y18H1A.11	NP_871893.1

Gene Ontology
Bibliography

Related articles in PubMed

Development of the Swimbladder Surfactant System and Biogenesis of Lysosome-Related Organelles Is Regulated by BLOS1 in Zebrafish.
Chen T, Song G, Yang H, Mao L, Cui Z, Huang K
Genetics(2018 Jan)

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Ma Z, Zhu P, Pang M, Guo L, Chang N, Zheng J, Zhu X, Gao C, Huang H, Cui Z, Xiong JW, Peng J, Chen J
Scientific reports7(1)10381(2017 Sep)

Identification of p53-target genes in Danio rerio.
Mandriani B, Castellana S, Rinaldi C, Manzoni M, Venuto S, Rodriguez-Aznar E, Galceran J, Nieto MA, Borsani G, Monti E, Mazza T, Merla G, Micale L
Scientific reports632474(2016 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pcyt1aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pcyt1aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33739	NM_001017634.1 Latest version!	Danio rerio phosphate cytidylyltransferase 1, choline, alpha a (pcyt1aa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa150317	XM_021480218.1 Latest version!	Danio rerio phosphate cytidylyltransferase 1, choline, alpha a (pcyt1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pcyt1aa ( NM_001017634.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017634.1 Danio rerio phosphate cytidylyltransferase 1, choline, alpha a (pcyt1aa), mRNA.

Price & Availability↑

CloneID	ODa33739
Clone ID Related Accession (Same CDS sequence)	NM_001017634.1
Accession Version	NM_001017634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	choline-phosphate cytidylyltransferase A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092816.1. ##Evidence-Data-START## Transcript exon combination :: BC092816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC ACGGTTCAGC GCGACTGTCA AGGAAGAGAA AGAGAGATGG TTCAAACGGA 
GAGCCAGAGG AAGGAGAAAA ACCACTGAAG TTCAAAAGAT GCACGGTGGG TCTGAAATAT 
CCAGCTCCTT ATGCAGATCA GCTCGAGTCA ATGGAAGATA AGCAGTATCA GCGTGTCACC 
ATAGAGGAAG CTCGCAGAGG CACGCCACCC GACAGACCTG TGCGGGTGTA CGCTGATGGG 
ATCTTCGACA TGTTCCACTC AGGACATGCT CGCGCTCTCA TGCAGGCTAA ATGCCTCTTT 
CCAAACACTC ACCTTATTGT AGGTGTTTGC AGTGATGACC TCACGCACAA GCTAAAGGGC 
TTTACTGTCA TGAACGAAGA CGAGCGCTAT GATGCTGTTA GTCACTGTCG CTATGTGGAT 
GAGGTGGTCC GGAATGCCCC ATGGACTCTT ACACCAGAGT TCCTCACCAA ACATCGGATT 
GACTTTGTGG CCCATGATGA TATCCCATAC TCCTCAGCTG ACAGTGATGA TGTTTACAAG 
CACATCAAAG ATGCAGGAAT GTTTGCACCA ACCCAAAGGA CAGAGGGCAT CTCCACCTCT 
GACATCATCA CGCGCATCGT GCGAGATTAT GATGTGTACG TCAGACGGAA TCTTCAGCGT 
GGCTACACTG CAAAAGAGCT GAACGTCAGC TTCATCAATG AAAAGAAGTA CAACCTTCAG 
GAGCGTGTGG ACAAGGTGAA GAAGAAGGTG AAGGACGTGG AGGAGAAAAG TAAAGAGTTT 
GTGCAGAAGG TGGAGGAGAA GAGCATCGAC CTGATCCAGA AATGGGAAGA GAAGTCCCGG 
GAATTCATTG GCAACTTCCT GCAGATGTTT GGACCGGAGG GAGCACTGAA GCACATGCTG 
AAGGAGGGTA AAGGTCGAAT GCTTCAGGCC ATCAGTCCAA GGCAGAGTCC CAGCAGCAGT 
CCCACACGAG AGCGCTCGCC TTCCCCAACC TTCCGTCTGC CCTTCTTCAA CAAAACCTCC 
ACGCCGTCCT CCCCGTCCTC ACACCACAGC AGCGCCCTGT CCGGCTACAC CTACGCCGCC 
CGTGGACAGG CCATCAGCGA GGACGAGGAG GACGACGAAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017634.1
CDS	100..1224
Translation	MEAHGSARLSRKRKRDGSNGEPEEGEKPLKFKRCTVGLKYPAPYADQLESMEDKQYQRVTIEEARRGTPPDRPVRVYADGIFDMFHSGHARALMQAKCLFPNTHLIVGVCSDDLTHKLKGFTVMNEDERYDAVSHCRYVDEVVRNAPWTLTPEFLTKHRIDFVAHDDIPYSSADSDDVYKHIKDAGMFAPTQRTEGISTSDIITRIVRDYDVYVRRNLQRGYTAKELNVSFINEKKYNLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEEKSREFIGNFLQMFGPEGALKHMLKEGKGRMLQAISPRQSPSSSPTRERSPSPTFRLPFFNKTSTPSSPSSHHSSALSGYTYAARGQAISEDEEDDED

Target ORF information:

RefSeq Version	NM_001017634.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphate cytidylyltransferase 1, choline, alpha a (pcyt1aa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017634.1

ORF Insert Sequence:

ATGGAGGCCC ACGGTTCAGC GCGACTGTCA AGGAAGAGAA AGAGAGATGG TTCAAACGGA 
GAGCCAGAGG AAGGAGAAAA ACCACTGAAG TTCAAAAGAT GCACGGTGGG TCTGAAATAT 
CCAGCTCCTT ATGCAGATCA GCTCGAGTCA ATGGAAGATA AGCAGTATCA GCGTGTCACC 
ATAGAGGAAG CTCGCAGAGG CACGCCACCC GACAGACCTG TGCGGGTGTA CGCTGATGGG 
ATCTTCGACA TGTTCCACTC AGGACATGCT CGCGCTCTCA TGCAGGCTAA ATGCCTCTTT 
CCAAACACTC ACCTTATTGT AGGTGTTTGC AGTGATGACC TCACGCACAA GCTAAAGGGC 
TTTACTGTCA TGAACGAAGA CGAGCGCTAT GATGCTGTTA GTCACTGTCG CTATGTGGAT 
GAGGTGGTCC GGAATGCCCC ATGGACTCTT ACACCAGAGT TCCTCACCAA ACATCGGATT 
GACTTTGTGG CCCATGATGA TATCCCATAC TCCTCAGCTG ACAGTGATGA TGTTTACAAG 
CACATCAAAG ATGCAGGAAT GTTTGCACCA ACCCAAAGGA CAGAGGGCAT CTCCACCTCT 
GACATCATCA CGCGCATCGT GCGAGATTAT GATGTGTACG TCAGACGGAA TCTTCAGCGT 
GGCTACACTG CAAAAGAGCT GAACGTCAGC TTCATCAATG AAAAGAAGTA CAACCTTCAG 
GAGCGTGTGG ACAAGGTGAA GAAGAAGGTG AAGGACGTGG AGGAGAAAAG TAAAGAGTTT 
GTGCAGAAGG TGGAGGAGAA GAGCATCGAC CTGATCCAGA AATGGGAAGA GAAGTCCCGG 
GAATTCATTG GCAACTTCCT GCAGATGTTT GGACCGGAGG GAGCACTGAA GCACATGCTG 
AAGGAGGGTA AAGGTCGAAT GCTTCAGGCC ATCAGTCCAA GGCAGAGTCC CAGCAGCAGT 
CCCACACGAG AGCGCTCGCC TTCCCCAACC TTCCGTCTGC CCTTCTTCAA CAAAACCTCC 
ACGCCGTCCT CCCCGTCCTC ACACCACAGC AGCGCCCTGT CCGGCTACAC CTACGCCGCC 
CGTGGACAGG CCATCAGCGA GGACGAGGAG GACGACGAAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pcyt1aa ( XM_021480218.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335893.1 Danio rerio phosphate cytidylyltransferase 1, choline, alpha a (pcyt1aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150317
Clone ID Related Accession (Same CDS sequence)	XM_021480218.1
Accession Version	XM_021480218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	choline-phosphate cytidylyltransferase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC ACGGTTCAGC GCGACTGTCA AGGAAGAGAA AGAGAGATGG TTCAAACGGA 
GAGCCAGAGG AAGGAGAAAA ACCACTGAAG TTCAAAAGAT GCACGGTGGG TCTGAAATAT 
CCAGCTCCTT ATGCAGATCA GCTCGAGTCA ATGGAAGATA AGCAGTATCA GCGTGTCACC 
ATAGAGGAAG CTCGCAGAGG CACGCCACCC GACAGACCTG TGCGGGTGTA CGCTGATGGG 
ATCTTCGACA TGTTCCACTC AGGACATGCT CGCGCTCTCA TGCAGGCTAA ATGCCTCTTT 
CCAAACACTC ACCTTATTGT AGGTGTTTGC AGTGATGACC TCACGCACAA GCTAAAGGGC 
TTCACCGTCA TGAACGAGGA CGAGCGCTAT GATGCTGTTA GTCACTGTCG CTATGTGGAT 
GAGGTGGTCC GGAATGCCCC ATGGACTCTT ACACCAGAGT TCCTCACCAA ACATCGGATT 
GACTTTGTGG CCCATGATGA TATCCCATAC TCCTCAGCTG ACAGTGATGA TGTTTACAAG 
CACATCAAAG ATGCAGGAAT GTTTGCACCA ACCCAAAGGA CAGAGGGCAT CTCCACCTCT 
GACATCATCA CGCGCATCGT GCGAGATTAT GATGTGTACG TCAGACGGAA TCTTCAGCGT 
GGCTACACTG CAAAAGAGCT GAACGTCAGC TTCATCAATG AAAAGAAGTA CAACCTTCAG 
GAGCGTGTGG ACAAGGTGAA GAAGAAGGTG AAGGACGTGG AGGAGAAAAG TAAAGAGTTT 
GTGCAGAAGG TGGAGGAGAA GAGCATCGAC CTGATCCAGA AATGGGAAGA GAAGTCCCGG 
GAATTCATTG GCAACTTCCT GCAGATGTTT GGACCGGAGG GAGCACTGAA GCACATGCTG 
AAGGAGGGTA AAGGTCGAAT GCTTCAGGCC ATCAGTCCAA GGCAGAGTCC CAGCAGCAGT 
CCCACACGAG AGCGCTCGCC TTCCCCAACC TTCCGTCTGC CCTTCTTCAA CAAAACCTCC 
CCGCCGTCCT CCCCATCCTC ACACCACAGC AGTGCCCTGT CCGGCTACAC CTACGCCGCC 
CGTGGACAGG CCATCAGCGA GGACGAGGAG GACGACGAAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335893.1
CDS	320..1444
Translation	MEAHGSARLSRKRKRDGSNGEPEEGEKPLKFKRCTVGLKYPAPYADQLESMEDKQYQRVTIEEARRGTPPDRPVRVYADGIFDMFHSGHARALMQAKCLFPNTHLIVGVCSDDLTHKLKGFTVMNEDERYDAVSHCRYVDEVVRNAPWTLTPEFLTKHRIDFVAHDDIPYSSADSDDVYKHIKDAGMFAPTQRTEGISTSDIITRIVRDYDVYVRRNLQRGYTAKELNVSFINEKKYNLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEEKSREFIGNFLQMFGPEGALKHMLKEGKGRMLQAISPRQSPSSSPTRERSPSPTFRLPFFNKTSPPSSPSSHHSSALSGYTYAARGQAISEDEEDDED

Target ORF information:

RefSeq Version	XM_021480218.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphate cytidylyltransferase 1, choline, alpha a (pcyt1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480218.1

ORF Insert Sequence:

ATGGAGGCCC ACGGTTCAGC GCGACTGTCA AGGAAGAGAA AGAGAGATGG TTCAAACGGA 
GAGCCAGAGG AAGGAGAAAA ACCACTGAAG TTCAAAAGAT GCACGGTGGG TCTGAAATAT 
CCAGCTCCTT ATGCAGATCA GCTCGAGTCA ATGGAAGATA AGCAGTATCA GCGTGTCACC 
ATAGAGGAAG CTCGCAGAGG CACGCCACCC GACAGACCTG TGCGGGTGTA CGCTGATGGG 
ATCTTCGACA TGTTCCACTC AGGACATGCT CGCGCTCTCA TGCAGGCTAA ATGCCTCTTT 
CCAAACACTC ACCTTATTGT AGGTGTTTGC AGTGATGACC TCACGCACAA GCTAAAGGGC 
TTCACCGTCA TGAACGAGGA CGAGCGCTAT GATGCTGTTA GTCACTGTCG CTATGTGGAT 
GAGGTGGTCC GGAATGCCCC ATGGACTCTT ACACCAGAGT TCCTCACCAA ACATCGGATT 
GACTTTGTGG CCCATGATGA TATCCCATAC TCCTCAGCTG ACAGTGATGA TGTTTACAAG 
CACATCAAAG ATGCAGGAAT GTTTGCACCA ACCCAAAGGA CAGAGGGCAT CTCCACCTCT 
GACATCATCA CGCGCATCGT GCGAGATTAT GATGTGTACG TCAGACGGAA TCTTCAGCGT 
GGCTACACTG CAAAAGAGCT GAACGTCAGC TTCATCAATG AAAAGAAGTA CAACCTTCAG 
GAGCGTGTGG ACAAGGTGAA GAAGAAGGTG AAGGACGTGG AGGAGAAAAG TAAAGAGTTT 
GTGCAGAAGG TGGAGGAGAA GAGCATCGAC CTGATCCAGA AATGGGAAGA GAAGTCCCGG 
GAATTCATTG GCAACTTCCT GCAGATGTTT GGACCGGAGG GAGCACTGAA GCACATGCTG 
AAGGAGGGTA AAGGTCGAAT GCTTCAGGCC ATCAGTCCAA GGCAGAGTCC CAGCAGCAGT 
CCCACACGAG AGCGCTCGCC TTCCCCAACC TTCCGTCTGC CCTTCTTCAA CAAAACCTCC 
CCGCCGTCCT CCCCATCCTC ACACCACAGC AGTGCCCTGT CCGGCTACAC CTACGCCGCC 
CGTGGACAGG CCATCAGCGA GGACGAGGAG GACGACGAAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development of the Swimbladder Surfactant System and Biogenesis of Lysosome-Related Organelles Is Regulated by BLOS1 in Zebrafish.
Genetics(2018 Jan)
Chen T,Song G,Yang H,Mao L,Cui Z,Huang K

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Scientific reports7(1)10381(2017 Sep)
Ma Z,Zhu P,Pang M,Guo L,Chang N,Zheng J,Zhu X,Gao C,Huang H,Cui Z,Xiong JW,Peng J,Chen J

Identification of p53-target genes in Danio rerio.
Scientific reports632474(2016 Sep)
Mandriani B,Castellana S,Rinaldi C,Manzoni M,Venuto S,Rodriguez-Aznar E,Galceran J,Nieto MA,Borsani G,Monti E,Mazza T,Merla G,Micale L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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pcyt1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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