efemp2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	efemp2a
Entrez Gene ID	494044
Full Name	EGF containing fibulin extracellular matrix protein 2a
Synonyms	efemp2,wu:fb22f10,wu:fc49d09,zgc:103659
General protein information	Preferred Names EGF containing fibulin extracellular matrix protein 2a Names EGF-containing fibulin-like extracellular matrix protein 2 EGF containing fibulin-like extracellular matrix protein 2a fbln4 fibulin 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008587.1	NP_001008587.1	EGF-containing fibulin-like extracellular matrix protein 2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	efemp2a	NP_001008587.1
Danio rerio (zebrafish)	efemp2b	NP_001153830.1
Danio rerio (zebrafish)	LOC100537581	XP_003200899.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins.
Yu F, Wang H, Liu W, Lu L
Fish & shellfish immunology48169-74(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genetic compensation induced by deleterious mutations but not gene knockdowns.
Rossi A, Kontarakis Z, Gerri C, Nolte H, H?lper S, Kr?ger M, Stainier DY
Nature524(7564)230-3(2015 Aug)

Stat3-Efemp2a modulates the fibrillar matrix for cohesive movement of prechordal plate progenitors.
Zhang T, Yin C, Qiao L, Jing L, Li H, Xiao C, Luo N, Lei S, Meng W, Zhu H, Liu J, Xu H, Mo X
Development (Cambridge, England)141(22)4332-42(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Efemp2a acts as a downstream effector of Stat3 to promote extracellular matrix configuration for coherent collective cell migrations in vivo.
Title: Stat3-Efemp2a modulates the fibrillar matrix for cohesive movement of prechordal plate progenitors.

The following efemp2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efemp2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02789	NM_001008587.1 Latest version!	Danio rerio EGF containing fibulin extracellular matrix protein 2a (efemp2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

efemp2a ( NM_001008587.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001008587.1 Danio rerio EGF containing fibulin extracellular matrix protein 2a (efemp2a), mRNA.

Price & Availability↑

CloneID	ODa02789
Clone ID Related Accession (Same CDS sequence)	NM_001008587.1
Accession Version	NM_001008587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	EGF-containing fibulin-like extracellular matrix protein 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086840.1. ##Evidence-Data-START## Transcript exon combination :: BC086840.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGGCCCG GGTGTGTTTT GCTCTTTTGT ATGTGCATCT CTGTGTTTCT CCACCGCGCT 
ATTTCACAGC CACCCACAGA AACTGACACC TACACGGAAT GTACAGATGG ATATCACTGG 
GGTTCACAGA CTCAGCACTG CAAAGATATA AATGAATGTG AGACAATCAC TGAAGCCTGT 
AAGGGAGAAA TGAAGTGCTT CAACCACTAC GGTGGATACC TGTGCCTCCC TCGCTCAGCC 
TCGGTCATCC CTGCCGCTGA GCCACCCAAC CAGATCGACC CGAGCCTGGA GAACAGAGTC 
AGCGAGCCTT TCAACTCGTG CCCTCCAGGT TTCGAGCCCC AGCAGGACAG CTGCGTGGAT 
GTGGATGAGT GTGAACGGGA CAGCCATGAC TGCCAGCCCA GTCAACAGTG TTTCAACACA 
GAAGGCTCCT TCTCCTGCCA GTGTCCAGAT GGTTATCGCA AAGTGGGCAC AGAATGCATT 
GACATTGACG AGTGCCGGTA CCGTTACTGT CAGCATCGCT GTGTTAATGT CCCTGGCTCT 
TTCTCCTGCC AGTGTGAGCC TGGTTTCCAG CTGGCTGGCA ACAATCGCTC CTGTATTGAT 
GTGGATGAGT GTGGTATGGG GGCCCCCTGC AGTCAGTGGT GTTATAATAC ATACGGGACG 
TTCCTGTGTC GATGTGAGCA GGGTTATGAG CTCGGGCCAG ATGGCTTCGC GTGTAACGAC 
ATAGACGAGT GCAGCTACTC CAGCTACCTG TGCCAATATC AGTGTGTGAA TGAGCATGGC 
AAATTCTCTT GCGTTTGTCC TGAAGGCTAT CAGCTCCTGG GCACTCGCCT CTGCCAAGAC 
ATAAATGAGT GTGAGACAGG AGCTCACCAG TGCTCCGACG GACAGACTTG TGTGAACATT 
CATGGAGGTC ACAAATGTGT TGACAACAAA AGCTGTCAGG ATCCCTACAT GCAAGTATCT 
GAAAATCGCT GCATGTGTCC AGTCTCCAAG CCTGCCTGTC GAGACATGCC GTACTCTATT 
GTCCATCGCT ACATGAGCAT CACATCAGAG CGCTCCGTCC CTTCAGACAT CTTTCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACACCTTCC GCATCCGCTC TGGAGATGAC 
AATGGAGACT TCTATATCAG ACAAATCAAC AACATCAGCG CCATGCTTGT TCTGGCCCGT 
GCCGTCAACG GCCCTCAAGA GTATGTGCTG GATCTCGAGA TGGTTTCTAT CAACCCACTC 
ATGAGCTACC ACAGCAGCTC TGGCCTGCGC TTAACTATCT ACGTTGGGCC TTACGCCTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008587.1
CDS	232..1554
Translation	MRPGCVLLFCMCISVFLHRAISQPPTETDTYTECTDGYHWGSQTQHCKDINECETITEACKGEMKCFNHYGGYLCLPRSASVIPAAEPPNQIDPSLENRVSEPFNSCPPGFEPQQDSCVDVDECERDSHDCQPSQQCFNTEGSFSCQCPDGYRKVGTECIDIDECRYRYCQHRCVNVPGSFSCQCEPGFQLAGNNRSCIDVDECGMGAPCSQWCYNTYGTFLCRCEQGYELGPDGFACNDIDECSYSSYLCQYQCVNEHGKFSCVCPEGYQLLGTRLCQDINECETGAHQCSDGQTCVNIHGGHKCVDNKSCQDPYMQVSENRCMCPVSKPACRDMPYSIVHRYMSITSERSVPSDIFQIQATSVYPGAYNTFRIRSGDDNGDFYIRQINNISAMLVLARAVNGPQEYVLDLEMVSINPLMSYHSSSGLRLTIYVGPYAF

Target ORF information:

RefSeq Version	NM_001008587.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio EGF containing fibulin extracellular matrix protein 2a (efemp2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008587.1

ORF Insert Sequence:

ATGCGGCCCG GGTGTGTTTT GCTCTTTTGT ATGTGCATCT CTGTGTTTCT CCACCGCGCT 
ATTTCACAGC CACCCACAGA AACTGACACC TACACGGAAT GTACAGATGG ATATCACTGG 
GGTTCACAGA CTCAGCACTG CAAAGATATA AATGAATGTG AGACAATCAC TGAAGCCTGT 
AAGGGAGAAA TGAAGTGCTT CAACCACTAC GGTGGATACC TGTGCCTCCC TCGCTCAGCC 
TCGGTCATCC CTGCCGCTGA GCCACCCAAC CAGATCGACC CGAGCCTGGA GAACAGAGTC 
AGCGAGCCTT TCAACTCGTG CCCTCCAGGT TTCGAGCCCC AGCAGGACAG CTGCGTGGAT 
GTGGATGAGT GTGAACGGGA CAGCCATGAC TGCCAGCCCA GTCAACAGTG TTTCAACACA 
GAAGGCTCCT TCTCCTGCCA GTGTCCAGAT GGTTATCGCA AAGTGGGCAC AGAATGCATT 
GACATTGACG AGTGCCGGTA CCGTTACTGT CAGCATCGCT GTGTTAATGT CCCTGGCTCT 
TTCTCCTGCC AGTGTGAGCC TGGTTTCCAG CTGGCTGGCA ACAATCGCTC CTGTATTGAT 
GTGGATGAGT GTGGTATGGG GGCCCCCTGC AGTCAGTGGT GTTATAATAC ATACGGGACG 
TTCCTGTGTC GATGTGAGCA GGGTTATGAG CTCGGGCCAG ATGGCTTCGC GTGTAACGAC 
ATAGACGAGT GCAGCTACTC CAGCTACCTG TGCCAATATC AGTGTGTGAA TGAGCATGGC 
AAATTCTCTT GCGTTTGTCC TGAAGGCTAT CAGCTCCTGG GCACTCGCCT CTGCCAAGAC 
ATAAATGAGT GTGAGACAGG AGCTCACCAG TGCTCCGACG GACAGACTTG TGTGAACATT 
CATGGAGGTC ACAAATGTGT TGACAACAAA AGCTGTCAGG ATCCCTACAT GCAAGTATCT 
GAAAATCGCT GCATGTGTCC AGTCTCCAAG CCTGCCTGTC GAGACATGCC GTACTCTATT 
GTCCATCGCT ACATGAGCAT CACATCAGAG CGCTCCGTCC CTTCAGACAT CTTTCAGATT 
CAGGCCACCA GTGTCTATCC TGGAGCCTAC AACACCTTCC GCATCCGCTC TGGAGATGAC 
AATGGAGACT TCTATATCAG ACAAATCAAC AACATCAGCG CCATGCTTGT TCTGGCCCGT 
GCCGTCAACG GCCCTCAAGA GTATGTGCTG GATCTCGAGA TGGTTTCTAT CAACCCACTC 
ATGAGCTACC ACAGCAGCTC TGGCCTGCGC TTAACTATCT ACGTTGGGCC TTACGCCTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins.
Fish & shellfish immunology48169-74(2016 Jan)
Yu F,Wang H,Liu W,Lu L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genetic compensation induced by deleterious mutations but not gene knockdowns.
Nature524(7564)230-3(2015 Aug)
Rossi A,Kontarakis Z,Gerri C,Nolte H,H?lper S,Kr?ger M,Stainier DY

Stat3-Efemp2a modulates the fibrillar matrix for cohesive movement of prechordal plate progenitors.
Development (Cambridge, England)141(22)4332-42(2014 Nov)
Zhang T,Yin C,Qiao L,Jing L,Li H,Xiao C,Luo N,Lei S,Meng W,Zhu H,Liu J,Xu H,Mo X

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efemp2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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