tnni2a.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnni2a.1
Entrez Gene ID	492493
Full Name	troponin I type 2a (skeletal, fast), tandem duplicate 1
Synonyms	zgc:103650
General protein information	Preferred Names troponin I type 2a (skeletal, fast), tandem duplicate 1 Names troponin I type 2a (skeletal, fast), tandem duplicate 1 tropI-1a troponin I, skeletal, fast 2a, tandem duplicate 1 troponin I, skeletal, fast 2a.1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007365.1	NP_001007366.1	troponin I type 2a (skeletal, fast), tandem duplicate 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnni2	NP_033431.1
Rattus norvegicus (Norway rat)	Tnni2	NP_058881.1
Danio rerio (zebrafish)	tnni2a.1	NP_001007366.1
Danio rerio (zebrafish)	tnni2a.4	NP_001009901.2
Pan troglodytes (chimpanzee)	TNNI2	XP_001153267.1
Bos taurus (cattle)	TNNI2	NP_001179023.1
Xenopus tropicalis (tropical clawed frog)	tnni2	NP_001011110.1
	LOC721041	XP_001117040.1
Canis lupus familiaris (dog)	TNNI2	XP_851068.1
Danio rerio (zebrafish)	tnni2a.3	NP_991138.2
Danio rerio (zebrafish)	tnni2b.1	NP_001129964.1
Danio rerio (zebrafish)	tnni2a.2	NP_957387.1
Homo sapiens (human)	TNNI2	NP_001139301.1
Gallus gallus (chicken)	TNNI2	NP_990748.1
Danio rerio (zebrafish)	tnni2b.2	NP_001003423.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional Divergence in Teleost Cardiac Troponin Paralogs Guides Variation in the Interaction of TnI Switch Region with TnC.
Genge CE, Stevens CM, Davidson WS, Singh G, Peter Tieleman D, Tibbits GF
Genome biology and evolution8(4)994-1011(2016 Apr)

"Young at heart": Regenerative potential linked to immature cardiac phenotypes.
Gomes RS, Skroblin P, Munster AB, Tomlins H, Langley SR, Zampetaki A, Yin X, Wardle FC, Mayr M
Journal of molecular and cellular cardiology92105-8(2016 Mar)

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Shih YH, Zhang Y, Ding Y, Ross CA, Li H, Olson TM, Xu X
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)

Microarray analysis of prothrombin knockdown in zebrafish.
Day KR, Jagadeeswaran P
Blood cells, molecules & diseases43(2)202-10(2009 Sep-Oct)

An update on MyoD evolution in teleosts and a proposed consensus nomenclature to accommodate the tetraploidization of different vertebrate genomes.
Macqueen DJ, Johnston IA
PloS one3(2)e1567(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tnni2a.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnni2a.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02553	NM_001007365.1 Latest version!	Danio rerio troponin I type 2a (skeletal, fast), tandem duplicate 1 (tnni2a.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnni2a.1 ( NM_001007365.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007366.1 Danio rerio troponin I type 2a (skeletal, fast), tandem duplicate 1 (tnni2a.1), mRNA.

Price & Availability↑

CloneID	ODa02553
Clone ID Related Accession (Same CDS sequence)	NM_001007365.1
Accession Version	NM_001007365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Danio rerio(zebrafish)
Product	troponin I type 2a (skeletal, fast), tandem duplicate 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085574.1. ##Evidence-Data-START## Transcript exon combination :: BC085574.1, EH455416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGAGA AAAAGATGTC ATCGAGTCGC AAGCATCATC TAAAGAGCTT GATGCTCTCC 
ATCGCTAAGG GTTTACTGGA GCAGGAGGTA AATGACAGAG AGGTGGAGAG GGAGAGATAC 
ATGGCAGAAA CCTGTCAGCC TCTTTCCCTA CCTGGGTCCA TGCAGGCATT ACAGGAACTG 
TGCAGAGATC TTCATCGAAA AATTGATGTG ATTGATGAGG AAAGATATGA TCTGGAAATG 
AAAGTCAACA AATGTGCAAA GGAGATCGAA GACCTGAATA TCAAAGTTAT TGACCTGAAG 
GGCAAGTTCA AGAAGCCAAC GCTGAGGAAA GTGCGCATGT CTGCCGATCA GATGCTTCAG 
GCTCTGCTGG GCTCCAAACA CAAGGTCTCT CTGGACTTGA GAGCAAACCT GAAACAAGTC 
AAGAAGGAGG TCAAAGAGGA GGATAAAGAA CTGCGTGATG TTGGTGACTG GCGTAAAAAT 
GTTGAAGACA AGGCTGGCAT GGACGGCAGG AAGAAGATGT TTGAAGCTGA GGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007366.1
CDS	76..612
Translation	MSEKKMSSSRKHHLKSLMLSIAKGLLEQEVNDREVERERYMAETCQPLSLPGSMQALQELCRDLHRKIDVIDEERYDLEMKVNKCAKEIEDLNIKVIDLKGKFKKPTLRKVRMSADQMLQALLGSKHKVSLDLRANLKQVKKEVKEEDKELRDVGDWRKNVEDKAGMDGRKKMFEAEA

Target ORF information:

RefSeq Version	NM_001007365.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio troponin I type 2a (skeletal, fast), tandem duplicate 1 (tnni2a.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007365.1

ORF Insert Sequence:

ATGTCGGAGA AAAAGATGTC ATCGAGTCGC AAGCATCATC TAAAGAGCTT GATGCTCTCC 
ATCGCTAAGG GTTTACTGGA GCAGGAGGTA AATGACAGAG AGGTGGAGAG GGAGAGATAC 
ATGGCAGAAA CCTGTCAGCC TCTTTCCCTA CCTGGGTCCA TGCAGGCATT ACAGGAACTG 
TGCAGAGATC TTCATCGAAA AATTGATGTG ATTGATGAGG AAAGATATGA TCTGGAAATG 
AAAGTCAACA AATGTGCAAA GGAGATCGAA GACCTGAATA TCAAAGTTAT TGACCTGAAG 
GGCAAGTTCA AGAAGCCAAC GCTGAGGAAA GTGCGCATGT CTGCCGATCA GATGCTTCAG 
GCTCTGCTGG GCTCCAAACA CAAGGTCTCT CTGGACTTGA GAGCAAACCT GAAACAAGTC 
AAGAAGGAGG TCAAAGAGGA GGATAAAGAA CTGCGTGATG TTGGTGACTG GCGTAAAAAT 
GTTGAAGACA AGGCTGGCAT GGACGGCAGG AAGAAGATGT TTGAAGCTGA GGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional Divergence in Teleost Cardiac Troponin Paralogs Guides Variation in the Interaction of TnI Switch Region with TnC.
Genome biology and evolution8(4)994-1011(2016 Apr)
Genge CE,Stevens CM,Davidson WS,Singh G,Peter Tieleman D,Tibbits GF

"Young at heart": Regenerative potential linked to immature cardiac phenotypes.
Journal of molecular and cellular cardiology92105-8(2016 Mar)
Gomes RS,Skroblin P,Munster AB,Tomlins H,Langley SR,Zampetaki A,Yin X,Wardle FC,Mayr M

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)
Shih YH,Zhang Y,Ding Y,Ross CA,Li H,Olson TM,Xu X

Microarray analysis of prothrombin knockdown in zebrafish.
Blood cells, molecules & diseases43(2)202-10(2009 Sep-Oct)
Day KR,Jagadeeswaran P

An update on MyoD evolution in teleosts and a proposed consensus nomenclature to accommodate the tetraploidization of different vertebrate genomes.
PloS one3(2)e1567(2008 Feb)
Macqueen DJ,Johnston IA