esco2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	esco2
Entrez Gene ID	445395
Full Name	establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms	si:dkey-217m5.6,zgc:111795
General protein information	Preferred Names establishment of sister chromatid cohesion N-acetyltransferase 2 Names N-acetyltransferase ESCO2 ECO1 homolog 2 establishment of cohesion 1 homolog 2 un-named hi2865 unm hi2865
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160818.2	XP_005160875.1	N-acetyltransferase ESCO2 isoform X1
XM_005160818.3	XP_005160875.1	N-acetyltransferase ESCO2 isoform X1
NM_001003872.1	NP_001003872.1	N-acetyltransferase ESCO2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ESCO2	XP_528296.2
Canis lupus familiaris (dog)	ESCO2	XP_543224.2
Mus musculus (house mouse)	Esco2	NP_082315.3
Rattus norvegicus (Norway rat)	Esco2	XP_006252234.1
Oryza sativa (rice)	Os04g0498900	NP_001053216.2
Xenopus tropicalis (tropical clawed frog)	esco2	NP_001121462.1
Homo sapiens (human)	ESCO2	NP_001017420.1
Bos taurus (cattle)	ESCO2	NP_001094652.1
Oryza sativa (rice)	Os05g0376300	NP_001055381.1
Gallus gallus (chicken)	ESCO2	XP_420012.3
Danio rerio (zebrafish)	esco2	NP_001003872.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011742	XP_320770.4
Arabidopsis thaliana (thale cress)	CTF7	NP_194868.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome.
Xu B, Gogol M, Gaudenz K, Gerton JL
BMC genomics1725(2016 Jan)

Esco2 regulates cx43 expression during skeletal regeneration in the zebrafish fin.
Banerji R, Eble DM, Iovine MK, Skibbens RV
Developmental dynamics : an official publication of the American Association of Anatomists245(1)7-21(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reveals the dramatic rescue effects of L-leucine stimulation of mTORC1 in RBS cells and supports that normal gene expression and translation requires ESCO2 function.
Title: Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome.

esco2 is upregulated during fin regeneration. esco2 knockdown adversely affects tissue and bone growth in regenerating fins-consistent with role in skeletal morphogenesis. knockdown diminishes cx43 expression which is required for cell-cell communication
Title: Esco2 regulates cx43 expression during skeletal regeneration in the zebrafish fin.

although many cells required Esco2 to establish cohesion, 10-20% of cells had only weakened cohesion in the absence of Esco2, suggesting that compensatory cohesion mechanisms exist in these cells that undergo a normal mitotic division.
Title: Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome.

Esco2 depleted zebrafish embryos exhibit features that resemble Roberts Syndrome, including mitotic defects, craniofacial abnormalities and limb truncations.
Title: A zebrafish model of Roberts syndrome reveals that Esco2 depletion interferes with development by disrupting the cell cycle.

The following esco2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the esco2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03696	NM_001003872.1 Latest version!	Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa25127	XM_005160818.3 Latest version!	Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa25127	XM_005160818.2	Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

esco2 ( NM_001003872.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003872.1 Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), mRNA.

Price & Availability↑

CloneID	ODa03696
Clone ID Related Accession (Same CDS sequence)	NM_001003872.1
Accession Version	NM_001003872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Danio rerio(zebrafish)
Product	N-acetyltransferase ESCO2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY648804.1. ##Evidence-Data-START## Transcript exon combination :: AY648804.1, BC096847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTATCCC GAAAGAGAAA ACATGGCTCT CCTGATGCCG AGAGTAATCC ATCGAAGAAA 
CAAATAACAA GCCTGAGGAG TTCTCCAAGA AGGACCACTC GACAAAAAGA AAACATTCCC 
ATTTCTCTGA ATTCCCCCCA AAAGATTCCC AGCACACCTA AAAAGACGCA GAGGGCCTTT 
TTGCTAGAAT CTCCCCCAAA GCGAGTCTCG CCACGGAAAG CAGTGCTGGG AGCAGGGACC 
TTCTACAGCA AGCAGAAACC CCTGTATCTC ACACCTCTGG AGAGAAAAGT GCTGAAAGAA 
GCCAAATCTC CACCATCAGT CACTAACAAA GAGCCGTCAC GCCCTCCTTT GACTGCTGCG 
AATCAGGTTG TAAAACCAGC CAAGAAAGTT CAGAAGAAAC CCAGAGCTTC TGCTCCACAG 
TCCAACCTAA AAGGCTACTT CACGGCTAAG CCCAAAGCGA CAAAAAGTTC GTCAGATAAA 
CAAACGGATC AAGTGCTGAA ATCCACAATG GCTCCCATTT CATTCAGCAG TATGAAGTCC 
AAAGGTAAAC CCAAGCTGGT TGTCGGCGCT GCTTTCTTCA ACACTGGAAA AAAGCCTACT 
TCAATGTATA AGAAGTCTGC ACAGAATACA AAACCTAAAC CAACATACGA AAAACCCAGC 
ATTCGGAAAC CGGTGAGGGA GAAGGAATTG GTGACGGCAC CAGGACAGCG TTCCCCAGTT 
CGCCGTGCTG TTTTCTTGAA AAAACAGCCA GAAGTGGAAG TTAGCCATGA TAAAAGAGAG 
TCGACACAAG CGCCAATGTC TCCTCAGGTC CTTGCAGATG TGCATGGCAT CACTAAAGAG 
CTGAGGGTGG TTTTGAGGAG ATCTGTGAGC CCTGAGACTG GCAGCCAGGA CGCTCCTTCT 
GAAGCTGATT CGGTGTTTGA TGTGAGTGAT TTACTGCTTC CAGATCATGA TAGCTCTCAT 
GATGAAGAAG AGTCATCTGT GTATCCCATC TTTGGCACTA AAATATTTCA GAAAAAGGGG 
AAGTTGTCTC CACCGCTGAA CAGCAGCACT CCTTCAGCTC TGACCGCAAC TCCTGCTCTT 
AAAGCCAAAG AGAGGAGCAT GCTGAGGAGA GAGATGAAGA AGCAAACCGA CAACCAGCTC 
ATCATCGATG CTGGTCAGAA GCAGTTTGGA GCCACTACCT GTGCATCCTG TGGGATGCTG 
TACAGCACAG ACAGTCCAGA GGACAACTTT CAACACACAC AGTTCCATCA GCGCTTTCTG 
GACACCATTA AGTTTGTGGG CTGGAAAAAA GAGAGGGTTG TGGCAGAATT CTGGGATGGA 
AAGATCATTC TCGTTCTTCC AGATGATCCC AAGTACGCCA CGAGAAAGGC AGAGGACGTG 
AGGCGGATTG CAGACAGTGA GCTGGGTTTT CAGCAGATCA CCCTCAGCAG CCCGAGCTCT 
GCTAAAACAT ACCTCTTCAT CAACACTGAC AGGATGGTTG TAGGATGTCT GGTGGCTGAG 
AATATTAGAC AGGCCTATCG TGTGCTGGAG CAGCAAGAGA AACAAAAAGA CATGAGCAAG 
GAGGACTTCA TGGAGCACCA CAGGACCTGG TGCTGCTCTA CTGTCCCGGA GAAAGCCCTC 
TGTGGCGTCA GTCGCATCTG GGTCTTCAGT CTGATGAGGA GGAAGAGCGT TGCCACACGC 
CTTCTGGACA CGGCCAGGAA TACGTTCATG TACGGAAGTC ACCTGACCAA AGAGGAAATA 
GCCTTTTCTG ACCCGACGCC ACAAGGGAAG CTGTTTGCTA CAAAGTACTG CCAAACACCA 
ACATTTCTGG TGTACAACTT CATCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003872.1
CDS	147..1976
Translation	MLSRKRKHGSPDAESNPSKKQITSLRSSPRRTTRQKENIPISLNSPQKIPSTPKKTQRAFLLESPPKRVSPRKAVLGAGTFYSKQKPLYLTPLERKVLKEAKSPPSVTNKEPSRPPLTAANQVVKPAKKVQKKPRASAPQSNLKGYFTAKPKATKSSSDKQTDQVLKSTMAPISFSSMKSKGKPKLVVGAAFFNTGKKPTSMYKKSAQNTKPKPTYEKPSIRKPVREKELVTAPGQRSPVRRAVFLKKQPEVEVSHDKRESTQAPMSPQVLADVHGITKELRVVLRRSVSPETGSQDAPSEADSVFDVSDLLLPDHDSSHDEEESSVYPIFGTKIFQKKGKLSPPLNSSTPSALTATPALKAKERSMLRREMKKQTDNQLIIDAGQKQFGATTCASCGMLYSTDSPEDNFQHTQFHQRFLDTIKFVGWKKERVVAEFWDGKIILVLPDDPKYATRKAEDVRRIADSELGFQQITLSSPSSAKTYLFINTDRMVVGCLVAENIRQAYRVLEQQEKQKDMSKEDFMEHHRTWCCSTVPEKALCGVSRIWVFSLMRRKSVATRLLDTARNTFMYGSHLTKEEIAFSDPTPQGKLFATKYCQTPTFLVYNFIS

Target ORF information:

RefSeq Version	NM_001003872.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003872.1

ORF Insert Sequence:

ATGTTATCCC GAAAGAGAAA ACATGGCTCT CCTGATGCCG AGAGTAATCC ATCGAAGAAA 
CAAATAACAA GCCTGAGGAG TTCTCCAAGA AGGACCACTC GACAAAAAGA AAACATTCCC 
ATTTCTCTGA ATTCCCCCCA AAAGATTCCC AGCACACCTA AAAAGACGCA GAGGGCCTTT 
TTGCTAGAAT CTCCCCCAAA GCGAGTCTCG CCACGGAAAG CAGTGCTGGG AGCAGGGACC 
TTCTACAGCA AGCAGAAACC CCTGTATCTC ACACCTCTGG AGAGAAAAGT GCTGAAAGAA 
GCCAAATCTC CACCATCAGT CACTAACAAA GAGCCGTCAC GCCCTCCTTT GACTGCTGCG 
AATCAGGTTG TAAAACCAGC CAAGAAAGTT CAGAAGAAAC CCAGAGCTTC TGCTCCACAG 
TCCAACCTAA AAGGCTACTT CACGGCTAAG CCCAAAGCGA CAAAAAGTTC GTCAGATAAA 
CAAACGGATC AAGTGCTGAA ATCCACAATG GCTCCCATTT CATTCAGCAG TATGAAGTCC 
AAAGGTAAAC CCAAGCTGGT TGTCGGCGCT GCTTTCTTCA ACACTGGAAA AAAGCCTACT 
TCAATGTATA AGAAGTCTGC ACAGAATACA AAACCTAAAC CAACATACGA AAAACCCAGC 
ATTCGGAAAC CGGTGAGGGA GAAGGAATTG GTGACGGCAC CAGGACAGCG TTCCCCAGTT 
CGCCGTGCTG TTTTCTTGAA AAAACAGCCA GAAGTGGAAG TTAGCCATGA TAAAAGAGAG 
TCGACACAAG CGCCAATGTC TCCTCAGGTC CTTGCAGATG TGCATGGCAT CACTAAAGAG 
CTGAGGGTGG TTTTGAGGAG ATCTGTGAGC CCTGAGACTG GCAGCCAGGA CGCTCCTTCT 
GAAGCTGATT CGGTGTTTGA TGTGAGTGAT TTACTGCTTC CAGATCATGA TAGCTCTCAT 
GATGAAGAAG AGTCATCTGT GTATCCCATC TTTGGCACTA AAATATTTCA GAAAAAGGGG 
AAGTTGTCTC CACCGCTGAA CAGCAGCACT CCTTCAGCTC TGACCGCAAC TCCTGCTCTT 
AAAGCCAAAG AGAGGAGCAT GCTGAGGAGA GAGATGAAGA AGCAAACCGA CAACCAGCTC 
ATCATCGATG CTGGTCAGAA GCAGTTTGGA GCCACTACCT GTGCATCCTG TGGGATGCTG 
TACAGCACAG ACAGTCCAGA GGACAACTTT CAACACACAC AGTTCCATCA GCGCTTTCTG 
GACACCATTA AGTTTGTGGG CTGGAAAAAA GAGAGGGTTG TGGCAGAATT CTGGGATGGA 
AAGATCATTC TCGTTCTTCC AGATGATCCC AAGTACGCCA CGAGAAAGGC AGAGGACGTG 
AGGCGGATTG CAGACAGTGA GCTGGGTTTT CAGCAGATCA CCCTCAGCAG CCCGAGCTCT 
GCTAAAACAT ACCTCTTCAT CAACACTGAC AGGATGGTTG TAGGATGTCT GGTGGCTGAG 
AATATTAGAC AGGCCTATCG TGTGCTGGAG CAGCAAGAGA AACAAAAAGA CATGAGCAAG 
GAGGACTTCA TGGAGCACCA CAGGACCTGG TGCTGCTCTA CTGTCCCGGA GAAAGCCCTC 
TGTGGCGTCA GTCGCATCTG GGTCTTCAGT CTGATGAGGA GGAAGAGCGT TGCCACACGC 
CTTCTGGACA CGGCCAGGAA TACGTTCATG TACGGAAGTC ACCTGACCAA AGAGGAAATA 
GCCTTTTCTG ACCCGACGCC ACAAGGGAAG CTGTTTGCTA CAAAGTACTG CCAAACACCA 
ACATTTCTGG TGTACAACTT CATCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esco2 ( XM_005160818.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160875.1 Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25127
Clone ID Related Accession (Same CDS sequence)	XM_005160818.2 , XM_005160818.3
Accession Version	XM_005160818.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	N-acetyltransferase ESCO2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCCC GAAAGAGAAA ACATGGCTCT CCTGATGCCG AGAGTAATCC ATCGAAGAAA 
CAAATAACAA GCCTGAGGAG TTCTCCAAGA AGGACCACTC GACAAAAAGA AAACATTCCC 
ATTTCTCTGA ATTCCCCCCA AAAGATTCCC AGCACACCTA AAAAGACGCA GAGGGCCTTT 
TTGCTAGAAT CTCCCCCAAA GCGAGTCTCG CCACGGAAAG CAGTGCTGGG AGCAGGGACC 
TTCTACAGCA AGCAGAAACC CCTGTATCTC ACACCTCTGG AGAGAAAAGT GCTGAAAGAA 
GCCAAATCTC CACCATCAGT CACTAACAAA GAGCCGTCAC GCCCTCCTTT GACTGCTGCG 
AATCAGGTTG TAAAACCAGC CAAGAAAGTT CAGAAGAAAC CCAGAGCTTC TGCTCCACAG 
TCCAACCTAA AAGGCTACTT CACGGCTAAG CCCAAAGCGA CAAAAAGTTC GTCAGATAAA 
CAAACGGATC AAGTGCTGAA ATCCACAATG GCTCCCATTT CATTCAGCAG TATGAAGTCC 
AAAGGTAAAC CCAAGCTGGT TGTCGGCGCT GCTTTCTTCA ACACTGGAAA AAAGCCTACT 
TCAATGTATA AGAAGTCTGC ACAGAATACA AAACCTAAAC CAACATACGA AAAACCCAGC 
ATTCGGAAAC CGGTGAGGGA GAAGGAATTG GTGACGGCAC CAGGACAGCG TTCCCCAGTT 
CGCCGTGCTG TTTTCTTGAA AAAACAGCCA GAAGTGGAAG TTAGCCATGA TAAAAGAGAG 
TCGACACAAG CGCCAATGTC TCCTCAGGTC CTTGCAGATG TGCATGGCAT CACTAAAGAG 
CTGAGGGTGG TTTTGAGGAG ATCTGTGAGC CCTGAGACTG GCAGCCAGGA CGCTCCTTCT 
GAAGCTGATT CGGTGTTTGA TGTGAGTGAT TTACTGCTTC CAGATCATGA TAGCTCTCAT 
GATGAAGAGT CATCTGTGTA TCCCATCTTT GGCACTAAAA GACCTCAGAA AAAGGGGAAG 
TTGTCTCCAC CGCTGAACAG CAGCACTCCT TCAGCTCTGA CCGCAACTCC TGCTCTTAAA 
GCCAAAGAGA GGAGCATGCT GAGGAGAGAG ATGAAGAAGC AAACCGACAA CCAGCTCATC 
ATCGATGCTG GTCAGAAGCA GTTTGGAGCC ACTACCTGTG CATCCTGTGG GATGCTGTAC 
AGCACAGACA GTCCAGAGGA CAACTTTCAA CACACACAGT TCCATCAGCG CTTTCTGGAC 
ACCATTAAGT TTGTGGGCTG GAAAAAAGAG AGGGTTGTGG CAGAATTCTG GGATGGAAAG 
ATCATTCTCG TTCTTCCAGA TGATCCCAAG TACGCCACGA GAAAGGCAGA GGACGTGAGG 
CGGATTGCAG ACAGTGAGCT GGGTTTTCAG CAGATCACCC TCAGCAGCCC GAGCTCTGCT 
AAAACATACC TCTTCATCAA CACTGACAGG ATGGTTGTAG GATGTCTGGT GGCTGAGAAT 
ATTAGACAGG CCTATCGTGT GCTGGAGCAG CAAGAGAAAC AAAAAGACAT GAGCAAGGAG 
GACTTCATGG AGCACCACAG GACCTGGTGC TGCTCTACTG TCCCGGAGAA AGCCCTCTGT 
GGCGTCAGTC GCATCTGGGT CTTCAGTCTG ATGAGGAGGA AGAGCGTTGC CACACGCCTT 
CTGGACACGG CCAGGAATAC GTTCATGTAC GGAAGTCACC TGACCAAAGA GGAAATAGCC 
TTTTCTGACC CGACGCCACA AGGGAAGCTG TTTGCTACAA AGTACTGCCA AACACCAACA 
TTTCTGGTGT ACAACTTCAT CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160875.1
CDS	337..2163
Translation	MLSRKRKHGSPDAESNPSKKQITSLRSSPRRTTRQKENIPISLNSPQKIPSTPKKTQRAFLLESPPKRVSPRKAVLGAGTFYSKQKPLYLTPLERKVLKEAKSPPSVTNKEPSRPPLTAANQVVKPAKKVQKKPRASAPQSNLKGYFTAKPKATKSSSDKQTDQVLKSTMAPISFSSMKSKGKPKLVVGAAFFNTGKKPTSMYKKSAQNTKPKPTYEKPSIRKPVREKELVTAPGQRSPVRRAVFLKKQPEVEVSHDKRESTQAPMSPQVLADVHGITKELRVVLRRSVSPETGSQDAPSEADSVFDVSDLLLPDHDSSHDEESSVYPIFGTKRPQKKGKLSPPLNSSTPSALTATPALKAKERSMLRREMKKQTDNQLIIDAGQKQFGATTCASCGMLYSTDSPEDNFQHTQFHQRFLDTIKFVGWKKERVVAEFWDGKIILVLPDDPKYATRKAEDVRRIADSELGFQQITLSSPSSAKTYLFINTDRMVVGCLVAENIRQAYRVLEQQEKQKDMSKEDFMEHHRTWCCSTVPEKALCGVSRIWVFSLMRRKSVATRLLDTARNTFMYGSHLTKEEIAFSDPTPQGKLFATKYCQTPTFLVYNFIS

Target ORF information:

RefSeq Version	XM_005160818.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160818.2

ORF Insert Sequence:

ATGTTATCCC GAAAGAGAAA ACATGGCTCT CCTGATGCCG AGAGTAATCC ATCGAAGAAA 
CAAATAACAA GCCTGAGGAG TTCTCCAAGA AGGACCACTC GACAAAAAGA AAACATTCCC 
ATTTCTCTGA ATTCCCCCCA AAAGATTCCC AGCACACCTA AAAAGACGCA GAGGGCCTTT 
TTGCTAGAAT CTCCCCCAAA GCGAGTCTCG CCACGGAAAG CAGTGCTGGG AGCAGGGACC 
TTCTACAGCA AGCAGAAACC CCTGTATCTC ACACCTCTGG AGAGAAAAGT GCTGAAAGAA 
GCCAAATCTC CACCATCAGT CACTAACAAA GAGCCGTCAC GCCCTCCTTT GACTGCTGCG 
AATCAGGTTG TAAAACCAGC CAAGAAAGTT CAGAAGAAAC CCAGAGCTTC TGCTCCACAG 
TCCAACCTAA AAGGCTACTT CACGGCTAAG CCCAAAGCGA CAAAAAGTTC GTCAGATAAA 
CAAACGGATC AAGTGCTGAA ATCCACAATG GCTCCCATTT CATTCAGCAG TATGAAGTCC 
AAAGGTAAAC CCAAGCTGGT TGTCGGCGCT GCTTTCTTCA ACACTGGAAA AAAGCCTACT 
TCAATGTATA AGAAGTCTGC ACAGAATACA AAACCTAAAC CAACATACGA AAAACCCAGC 
ATTCGGAAAC CGGTGAGGGA GAAGGAATTG GTGACGGCAC CAGGACAGCG TTCCCCAGTT 
CGCCGTGCTG TTTTCTTGAA AAAACAGCCA GAAGTGGAAG TTAGCCATGA TAAAAGAGAG 
TCGACACAAG CGCCAATGTC TCCTCAGGTC CTTGCAGATG TGCATGGCAT CACTAAAGAG 
CTGAGGGTGG TTTTGAGGAG ATCTGTGAGC CCTGAGACTG GCAGCCAGGA CGCTCCTTCT 
GAAGCTGATT CGGTGTTTGA TGTGAGTGAT TTACTGCTTC CAGATCATGA TAGCTCTCAT 
GATGAAGAGT CATCTGTGTA TCCCATCTTT GGCACTAAAA GACCTCAGAA AAAGGGGAAG 
TTGTCTCCAC CGCTGAACAG CAGCACTCCT TCAGCTCTGA CCGCAACTCC TGCTCTTAAA 
GCCAAAGAGA GGAGCATGCT GAGGAGAGAG ATGAAGAAGC AAACCGACAA CCAGCTCATC 
ATCGATGCTG GTCAGAAGCA GTTTGGAGCC ACTACCTGTG CATCCTGTGG GATGCTGTAC 
AGCACAGACA GTCCAGAGGA CAACTTTCAA CACACACAGT TCCATCAGCG CTTTCTGGAC 
ACCATTAAGT TTGTGGGCTG GAAAAAAGAG AGGGTTGTGG CAGAATTCTG GGATGGAAAG 
ATCATTCTCG TTCTTCCAGA TGATCCCAAG TACGCCACGA GAAAGGCAGA GGACGTGAGG 
CGGATTGCAG ACAGTGAGCT GGGTTTTCAG CAGATCACCC TCAGCAGCCC GAGCTCTGCT 
AAAACATACC TCTTCATCAA CACTGACAGG ATGGTTGTAG GATGTCTGGT GGCTGAGAAT 
ATTAGACAGG CCTATCGTGT GCTGGAGCAG CAAGAGAAAC AAAAAGACAT GAGCAAGGAG 
GACTTCATGG AGCACCACAG GACCTGGTGC TGCTCTACTG TCCCGGAGAA AGCCCTCTGT 
GGCGTCAGTC GCATCTGGGT CTTCAGTCTG ATGAGGAGGA AGAGCGTTGC CACACGCCTT 
CTGGACACGG CCAGGAATAC GTTCATGTAC GGAAGTCACC TGACCAAAGA GGAAATAGCC 
TTTTCTGACC CGACGCCACA AGGGAAGCTG TTTGCTACAA AGTACTGCCA AACACCAACA 
TTTCTGGTGT ACAACTTCAT CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esco2 ( XM_005160818.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160875.1 Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25127
Clone ID Related Accession (Same CDS sequence)	XM_005160818.2 , XM_005160818.3
Accession Version	XM_005160818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	N-acetyltransferase ESCO2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCCC GAAAGAGAAA ACATGGCTCT CCTGATGCCG AGAGTAATCC ATCGAAGAAA 
CAAATAACAA GCCTGAGGAG TTCTCCAAGA AGGACCACTC GACAAAAAGA AAACATTCCC 
ATTTCTCTGA ATTCCCCCCA AAAGATTCCC AGCACACCTA AAAAGACGCA GAGGGCCTTT 
TTGCTAGAAT CTCCCCCAAA GCGAGTCTCG CCACGGAAAG CAGTGCTGGG AGCAGGGACC 
TTCTACAGCA AGCAGAAACC CCTGTATCTC ACACCTCTGG AGAGAAAAGT GCTGAAAGAA 
GCCAAATCTC CACCATCAGT CACTAACAAA GAGCCGTCAC GCCCTCCTTT GACTGCTGCG 
AATCAGGTTG TAAAACCAGC CAAGAAAGTT CAGAAGAAAC CCAGAGCTTC TGCTCCACAG 
TCCAACCTAA AAGGCTACTT CACGGCTAAG CCCAAAGCGA CAAAAAGTTC GTCAGATAAA 
CAAACGGATC AAGTGCTGAA ATCCACAATG GCTCCCATTT CATTCAGCAG TATGAAGTCC 
AAAGGTAAAC CCAAGCTGGT TGTCGGCGCT GCTTTCTTCA ACACTGGAAA AAAGCCTACT 
TCAATGTATA AGAAGTCTGC ACAGAATACA AAACCTAAAC CAACATACGA AAAACCCAGC 
ATTCGGAAAC CGGTGAGGGA GAAGGAATTG GTGACGGCAC CAGGACAGCG TTCCCCAGTT 
CGCCGTGCTG TTTTCTTGAA AAAACAGCCA GAAGTGGAAG TTAGCCATGA TAAAAGAGAG 
TCGACACAAG CGCCAATGTC TCCTCAGGTC CTTGCAGATG TGCATGGCAT CACTAAAGAG 
CTGAGGGTGG TTTTGAGGAG ATCTGTGAGC CCTGAGACTG GCAGCCAGGA CGCTCCTTCT 
GAAGCTGATT CGGTGTTTGA TGTGAGTGAT TTACTGCTTC CAGATCATGA TAGCTCTCAT 
GATGAAGAGT CATCTGTGTA TCCCATCTTT GGCACTAAAA GACCTCAGAA AAAGGGGAAG 
TTGTCTCCAC CGCTGAACAG CAGCACTCCT TCAGCTCTGA CCGCAACTCC TGCTCTTAAA 
GCCAAAGAGA GGAGCATGCT GAGGAGAGAG ATGAAGAAGC AAACCGACAA CCAGCTCATC 
ATCGATGCTG GTCAGAAGCA GTTTGGAGCC ACTACCTGTG CATCCTGTGG GATGCTGTAC 
AGCACAGACA GTCCAGAGGA CAACTTTCAA CACACACAGT TCCATCAGCG CTTTCTGGAC 
ACCATTAAGT TTGTGGGCTG GAAAAAAGAG AGGGTTGTGG CAGAATTCTG GGATGGAAAG 
ATCATTCTCG TTCTTCCAGA TGATCCCAAG TACGCCACGA GAAAGGCAGA GGACGTGAGG 
CGGATTGCAG ACAGTGAGCT GGGTTTTCAG CAGATCACCC TCAGCAGCCC GAGCTCTGCT 
AAAACATACC TCTTCATCAA CACTGACAGG ATGGTTGTAG GATGTCTGGT GGCTGAGAAT 
ATTAGACAGG CCTATCGTGT GCTGGAGCAG CAAGAGAAAC AAAAAGACAT GAGCAAGGAG 
GACTTCATGG AGCACCACAG GACCTGGTGC TGCTCTACTG TCCCGGAGAA AGCCCTCTGT 
GGCGTCAGTC GCATCTGGGT CTTCAGTCTG ATGAGGAGGA AGAGCGTTGC CACACGCCTT 
CTGGACACGG CCAGGAATAC GTTCATGTAC GGAAGTCACC TGACCAAAGA GGAAATAGCC 
TTTTCTGACC CGACGCCACA AGGGAAGCTG TTTGCTACAA AGTACTGCCA AACACCAACA 
TTTCTGGTGT ACAACTTCAT CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160875.1
CDS	161..1987
Translation	MLSRKRKHGSPDAESNPSKKQITSLRSSPRRTTRQKENIPISLNSPQKIPSTPKKTQRAFLLESPPKRVSPRKAVLGAGTFYSKQKPLYLTPLERKVLKEAKSPPSVTNKEPSRPPLTAANQVVKPAKKVQKKPRASAPQSNLKGYFTAKPKATKSSSDKQTDQVLKSTMAPISFSSMKSKGKPKLVVGAAFFNTGKKPTSMYKKSAQNTKPKPTYEKPSIRKPVREKELVTAPGQRSPVRRAVFLKKQPEVEVSHDKRESTQAPMSPQVLADVHGITKELRVVLRRSVSPETGSQDAPSEADSVFDVSDLLLPDHDSSHDEESSVYPIFGTKRPQKKGKLSPPLNSSTPSALTATPALKAKERSMLRREMKKQTDNQLIIDAGQKQFGATTCASCGMLYSTDSPEDNFQHTQFHQRFLDTIKFVGWKKERVVAEFWDGKIILVLPDDPKYATRKAEDVRRIADSELGFQQITLSSPSSAKTYLFINTDRMVVGCLVAENIRQAYRVLEQQEKQKDMSKEDFMEHHRTWCCSTVPEKALCGVSRIWVFSLMRRKSVATRLLDTARNTFMYGSHLTKEEIAFSDPTPQGKLFATKYCQTPTFLVYNFIS

Target ORF information:

RefSeq Version	XM_005160818.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160818.3

ORF Insert Sequence:

ATGTTATCCC GAAAGAGAAA ACATGGCTCT CCTGATGCCG AGAGTAATCC ATCGAAGAAA 
CAAATAACAA GCCTGAGGAG TTCTCCAAGA AGGACCACTC GACAAAAAGA AAACATTCCC 
ATTTCTCTGA ATTCCCCCCA AAAGATTCCC AGCACACCTA AAAAGACGCA GAGGGCCTTT 
TTGCTAGAAT CTCCCCCAAA GCGAGTCTCG CCACGGAAAG CAGTGCTGGG AGCAGGGACC 
TTCTACAGCA AGCAGAAACC CCTGTATCTC ACACCTCTGG AGAGAAAAGT GCTGAAAGAA 
GCCAAATCTC CACCATCAGT CACTAACAAA GAGCCGTCAC GCCCTCCTTT GACTGCTGCG 
AATCAGGTTG TAAAACCAGC CAAGAAAGTT CAGAAGAAAC CCAGAGCTTC TGCTCCACAG 
TCCAACCTAA AAGGCTACTT CACGGCTAAG CCCAAAGCGA CAAAAAGTTC GTCAGATAAA 
CAAACGGATC AAGTGCTGAA ATCCACAATG GCTCCCATTT CATTCAGCAG TATGAAGTCC 
AAAGGTAAAC CCAAGCTGGT TGTCGGCGCT GCTTTCTTCA ACACTGGAAA AAAGCCTACT 
TCAATGTATA AGAAGTCTGC ACAGAATACA AAACCTAAAC CAACATACGA AAAACCCAGC 
ATTCGGAAAC CGGTGAGGGA GAAGGAATTG GTGACGGCAC CAGGACAGCG TTCCCCAGTT 
CGCCGTGCTG TTTTCTTGAA AAAACAGCCA GAAGTGGAAG TTAGCCATGA TAAAAGAGAG 
TCGACACAAG CGCCAATGTC TCCTCAGGTC CTTGCAGATG TGCATGGCAT CACTAAAGAG 
CTGAGGGTGG TTTTGAGGAG ATCTGTGAGC CCTGAGACTG GCAGCCAGGA CGCTCCTTCT 
GAAGCTGATT CGGTGTTTGA TGTGAGTGAT TTACTGCTTC CAGATCATGA TAGCTCTCAT 
GATGAAGAGT CATCTGTGTA TCCCATCTTT GGCACTAAAA GACCTCAGAA AAAGGGGAAG 
TTGTCTCCAC CGCTGAACAG CAGCACTCCT TCAGCTCTGA CCGCAACTCC TGCTCTTAAA 
GCCAAAGAGA GGAGCATGCT GAGGAGAGAG ATGAAGAAGC AAACCGACAA CCAGCTCATC 
ATCGATGCTG GTCAGAAGCA GTTTGGAGCC ACTACCTGTG CATCCTGTGG GATGCTGTAC 
AGCACAGACA GTCCAGAGGA CAACTTTCAA CACACACAGT TCCATCAGCG CTTTCTGGAC 
ACCATTAAGT TTGTGGGCTG GAAAAAAGAG AGGGTTGTGG CAGAATTCTG GGATGGAAAG 
ATCATTCTCG TTCTTCCAGA TGATCCCAAG TACGCCACGA GAAAGGCAGA GGACGTGAGG 
CGGATTGCAG ACAGTGAGCT GGGTTTTCAG CAGATCACCC TCAGCAGCCC GAGCTCTGCT 
AAAACATACC TCTTCATCAA CACTGACAGG ATGGTTGTAG GATGTCTGGT GGCTGAGAAT 
ATTAGACAGG CCTATCGTGT GCTGGAGCAG CAAGAGAAAC AAAAAGACAT GAGCAAGGAG 
GACTTCATGG AGCACCACAG GACCTGGTGC TGCTCTACTG TCCCGGAGAA AGCCCTCTGT 
GGCGTCAGTC GCATCTGGGT CTTCAGTCTG ATGAGGAGGA AGAGCGTTGC CACACGCCTT 
CTGGACACGG CCAGGAATAC GTTCATGTAC GGAAGTCACC TGACCAAAGA GGAAATAGCC 
TTTTCTGACC CGACGCCACA AGGGAAGCTG TTTGCTACAA AGTACTGCCA AACACCAACA 
TTTCTGGTGT ACAACTTCAT CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome.
BMC genomics1725(2016 Jan)
Xu B,Gogol M,Gaudenz K,Gerton JL

Esco2 regulates cx43 expression during skeletal regeneration in the zebrafish fin.
Developmental dynamics : an official publication of the American Association of Anatomists245(1)7-21(2016 Jan)
Banerji R,Eble DM,Iovine MK,Skibbens RV

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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esco2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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