esco2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	esco2
Entrez Gene ID	100158558
Full Name	establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms	efo2,rbs
General protein information	Preferred Names establishment of sister chromatid cohesion N-acetyltransferase 2 Names N-acetyltransferase ESCO2 establishment of cohesion 1 homolog 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964016.3	XP_012819470.1	N-acetyltransferase ESCO2 isoform X1
NM_001127990.1	NP_001121462.1	N-acetyltransferase ESCO2
XM_012964017.3	XP_012819471.1	N-acetyltransferase ESCO2 isoform X2
XM_012964016.2	XP_012819470.1	N-acetyltransferase ESCO2 isoform X1
XM_012964017.2	XP_012819471.1	N-acetyltransferase ESCO2 isoform X2
XM_031901549.1	XP_031757409.1	N-acetyltransferase ESCO2 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ESCO2	XP_528296.2
Canis lupus familiaris (dog)	ESCO2	XP_543224.2
Mus musculus (house mouse)	Esco2	NP_082315.3
Rattus norvegicus (Norway rat)	Esco2	XP_006252234.1
Oryza sativa (rice)	Os04g0498900	NP_001053216.2
Xenopus tropicalis (tropical clawed frog)	esco2	NP_001121462.1
Homo sapiens (human)	ESCO2	NP_001017420.1
Bos taurus (cattle)	ESCO2	NP_001094652.1
Oryza sativa (rice)	Os05g0376300	NP_001055381.1
Gallus gallus (chicken)	ESCO2	XP_420012.3
Danio rerio (zebrafish)	esco2	NP_001003872.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011742	XP_320770.4
Arabidopsis thaliana (thale cress)	CTF7	NP_194868.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following esco2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the esco2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa57912	XM_012964016.3 Latest version!	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa57913	XM_012964017.3 Latest version!	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa57913	XM_031901549.1 Latest version!	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa24555	NM_001127990.1 Latest version!	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OXa32184	XM_012964016.2 Latest version!	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OXa32185	XM_012964017.2 Latest version!	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

esco2 ( XM_012964016.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819470.1 Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa57912
Clone ID Related Accession (Same CDS sequence)	XM_012964016.3
Accession Version	XM_012964016.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	N-acetyltransferase ESCO2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964016.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
TCCACCCCAT CTCCCTGCAG TGACAAGGGG GCAGGGGTAA ATACTCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCATCTCCCC GCAGTGACAA GGGGGCAGGG GTAAATACCC TTCCTTCTAA GCCCACCCCA 
TCTCCCCGCA GTGACAAGGG GGCAGGGGTA AATACCCTTC CTTCTAAGCC CACCCCAGAA 
GTAGAGGAAA GCGCCGGTGT CCCCAGCATG AAGACCACCT TTCTGGGGCT CAAGATGAAG 
CCGAGACCAA AACTCACGGT CGGAGCAGCA TTTTTTGCAA CGGGCAAGAG ACCCCACTCT 
GCTCCCAAGA GACCGTCCCC CAATATCAAG TTTCCCCCAT TGGCAAAGCC GCCGAAGCAG 
AGCACTGTAC CCATCACCAA AGCATTACCG CCGCACAGTG CTCTTAAAGG GGTTGTTTGT 
GTGGCTGAAG GGCAGAAGCA GGGGCAAGAA GGGAGGAAGG CGTGCAACGT ACCCTCTGAA 
TCTGTGGCCA AAAAGCCTGA AAGCAACAAG GAAGTCAAAC TGACGAAGGA CCATGCTGGC 
CAAGAGAGCC TACCTACCTC TCCAGCACAA GCAGGGACTG GCATGCAGCG AGCGGCGCCG 
GACTCTCCTG TGATAGACGA GGGTGAGGAC AGCTCTGTAC CAAAACCGTC CAATAAGAAA 
GGTCCAACTG TCTTTCCAAT CTTCAGTACC CCTGCGGGCA ATAAGAGACC TTTGGACCTG 
AGAGGAGACC TGGCCTCCCC TGTGTTCTCC AGCACCCCGA CTAATGCCCC CGCTGCCCTC 
AGGTTCCCCA AACAGGGCAA GAAGAAGAGA GAAGGAAGGA AGGTGGCAGA GGACCAGCTC 
GTCATTGATG CAGGACAGAA GCACTTTGGG CCAATAGCGT GCAACACGTG TGGGATGGTC 
TACGCTGCGG CCAATCTGGA GGATGAAGCT CAGCACGTCC AGTATCACCA GAGGCTTCTA 
GAGGGAATCC GATATGTGGG CTGGAAGAAA GAGCGAGTTA TTACCGAATT CTGGGATGGA 
AAAATCATCA TGGTTTCACC GGACGACCCT AAATACGCCC TGAAAAAGGC AGAGGAAGTT 
CGGGAGCTGG TGGATTCGGA GCTGGGATTT CAGCAGGTCT CCCTACGGTG CCCAAGCCAG 
ACCAGAACCT ACATGTTTGT GTCCAATGAG AAGAAGATTG TTGGCTGTTT GATTGCAGAG 
CCAATCAGAG AGGCATACAG AGTCCTCGCA GAACCCCCAT CTCCTCACTC GGTACATCGG 
GAACCTCTAG AACGCCATCG AGCTTGGAGG TGCTCAACCG AACCGGAACC TGCTATATGT 
GGTATCAGCA GGATTTGGGT GTTTGCCCTG ATGCGCAGAA AGGCCATCGC CAGCCGATTG 
GTGGATTCAG TCAGAAGTTC CTTTATGTAC GGCTCGGTCC TTACCACGGA AGAAATCGCC 
TTCTCCGACC CAACCCCCGA CGGCAAGCTG TTTGCTAGCA CCTACTGCAA GGTGCCGGAT 
TTCCTGGTCT ATAATTTTGT CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819470.1
CDS	221..2587
Translation	MAALTPRKRKRSSVSSDGRQAFYGTPVKKLIVEYDDCPSPLTTRNSSRRNIRKSPPVTTYTNMADPTEKENVGASPLKSNAARKLVVSPIQTAPAPKAAYGNSPLTASPRAASPFKSSVSTRSFYKRDKMYVTPLDRKLINESKSSLSSGNESSVSSPTSPVATIAPRAKRNKKQPGKRKSGPKGKKTPPGSGKKAINVPARKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPEVEESAGVPSMKTTFLGLKMKPRPKLTVGAAFFATGKRPHSAPKRPSPNIKFPPLAKPPKQSTVPITKALPPHSALKGVVCVAEGQKQGQEGRKACNVPSESVAKKPESNKEVKLTKDHAGQESLPTSPAQAGTGMQRAAPDSPVIDEGEDSSVPKPSNKKGPTVFPIFSTPAGNKRPLDLRGDLASPVFSSTPTNAPAALRFPKQGKKKREGRKVAEDQLVIDAGQKHFGPIACNTCGMVYAAANLEDEAQHVQYHQRLLEGIRYVGWKKERVITEFWDGKIIMVSPDDPKYALKKAEEVRELVDSELGFQQVSLRCPSQTRTYMFVSNEKKIVGCLIAEPIREAYRVLAEPPSPHSVHREPLERHRAWRCSTEPEPAICGISRIWVFALMRRKAIASRLVDSVRSSFMYGSVLTTEEIAFSDPTPDGKLFASTYCKVPDFLVYNFVS

Target ORF information:

RefSeq Version	XM_012964016.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964016.3

ORF Insert Sequence:

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
TCCACCCCAT CTCCCTGCAG TGACAAGGGG GCAGGGGTAA ATACTCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCATCTCCCC GCAGTGACAA GGGGGCAGGG GTAAATACCC TTCCTTCTAA GCCCACCCCA 
TCTCCCCGCA GTGACAAGGG GGCAGGGGTA AATACCCTTC CTTCTAAGCC CACCCCAGAA 
GTAGAGGAAA GCGCCGGTGT CCCCAGCATG AAGACCACCT TTCTGGGGCT CAAGATGAAG 
CCGAGACCAA AACTCACGGT CGGAGCAGCA TTTTTTGCAA CGGGCAAGAG ACCCCACTCT 
GCTCCCAAGA GACCGTCCCC CAATATCAAG TTTCCCCCAT TGGCAAAGCC GCCGAAGCAG 
AGCACTGTAC CCATCACCAA AGCATTACCG CCGCACAGTG CTCTTAAAGG GGTTGTTTGT 
GTGGCTGAAG GGCAGAAGCA GGGGCAAGAA GGGAGGAAGG CGTGCAACGT ACCCTCTGAA 
TCTGTGGCCA AAAAGCCTGA AAGCAACAAG GAAGTCAAAC TGACGAAGGA CCATGCTGGC 
CAAGAGAGCC TACCTACCTC TCCAGCACAA GCAGGGACTG GCATGCAGCG AGCGGCGCCG 
GACTCTCCTG TGATAGACGA GGGTGAGGAC AGCTCTGTAC CAAAACCGTC CAATAAGAAA 
GGTCCAACTG TCTTTCCAAT CTTCAGTACC CCTGCGGGCA ATAAGAGACC TTTGGACCTG 
AGAGGAGACC TGGCCTCCCC TGTGTTCTCC AGCACCCCGA CTAATGCCCC CGCTGCCCTC 
AGGTTCCCCA AACAGGGCAA GAAGAAGAGA GAAGGAAGGA AGGTGGCAGA GGACCAGCTC 
GTCATTGATG CAGGACAGAA GCACTTTGGG CCAATAGCGT GCAACACGTG TGGGATGGTC 
TACGCTGCGG CCAATCTGGA GGATGAAGCT CAGCACGTCC AGTATCACCA GAGGCTTCTA 
GAGGGAATCC GATATGTGGG CTGGAAGAAA GAGCGAGTTA TTACCGAATT CTGGGATGGA 
AAAATCATCA TGGTTTCACC GGACGACCCT AAATACGCCC TGAAAAAGGC AGAGGAAGTT 
CGGGAGCTGG TGGATTCGGA GCTGGGATTT CAGCAGGTCT CCCTACGGTG CCCAAGCCAG 
ACCAGAACCT ACATGTTTGT GTCCAATGAG AAGAAGATTG TTGGCTGTTT GATTGCAGAG 
CCAATCAGAG AGGCATACAG AGTCCTCGCA GAACCCCCAT CTCCTCACTC GGTACATCGG 
GAACCTCTAG AACGCCATCG AGCTTGGAGG TGCTCAACCG AACCGGAACC TGCTATATGT 
GGTATCAGCA GGATTTGGGT GTTTGCCCTG ATGCGCAGAA AGGCCATCGC CAGCCGATTG 
GTGGATTCAG TCAGAAGTTC CTTTATGTAC GGCTCGGTCC TTACCACGGA AGAAATCGCC 
TTCTCCGACC CAACCCCCGA CGGCAAGCTG TTTGCTAGCA CCTACTGCAA GGTGCCGGAT 
TTCCTGGTCT ATAATTTTGT CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esco2 ( XM_012964017.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819471.1 Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa57913
Clone ID Related Accession (Same CDS sequence)	XM_012964017.3 , XM_031901549.1
Accession Version	XM_012964017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	N-acetyltransferase ESCO2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
CCCACCCCAT CTCCCCGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTAG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCC 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCA TTACCGCCGC ACAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAAGGACCAT 
GCTGGCCAAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT CTCCTGTGAT AGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGTACCCCTG CGGGCAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCCGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAA CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCACCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CGGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819471.1
CDS	221..2473
Translation	MAALTPRKRKRSSVSSDGRQAFYGTPVKKLIVEYDDCPSPLTTRNSSRRNIRKSPPVTTYTNMADPTEKENVGASPLKSNAARKLVVSPIQTAPAPKAAYGNSPLTASPRAASPFKSSVSTRSFYKRDKMYVTPLDRKLINESKSSLSSGNESSVSSPTSPVATIAPRAKRNKKQPGKRKSGPKGKKTPPGSGKKAINVPARKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPEVEESAGVPSMKTTFLGLKMKPRPKLTVGAAFFATGKRPHSAPKRPSPNIKFPPLAKPPKQSTVPITKALPPHSALKGVVCVAEGQKQGQEGRKACNVPSESVAKKPESNKEVKLTKDHAGQESLPTSPAQAGTGMQRAAPDSPVIDEGEDSSVPKPSNKKGPTVFPIFSTPAGNKRPLDLRGDLASPVFSSTPTNAPAALRFPKQGKKKREGRKVAEDQLVIDAGQKHFGPIACNTCGMVYAAANLEDEAQHVQYHQRLLEGIRYVGWKKERVITEFWDGKIIMVSPDDPKYALKKAEEVRELVDSELGFQQVSLRCPSQTRTYMFVSNEKKIVGCLIAEPIREAYRVLAEPPSPHSVHREPLERHRAWRCSTEPEPAICGISRIWVFALMRRKAIASRLVDSVRSSFMYGSVLTTEEIAFSDPTPDGKLFASTYCKVPDFLVYNFVS

Target ORF information:

RefSeq Version	XM_012964017.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964017.3

ORF Insert Sequence:

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
CCCACCCCAT CTCCCCGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTAG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCC 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCA TTACCGCCGC ACAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAAGGACCAT 
GCTGGCCAAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT CTCCTGTGAT AGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGTACCCCTG CGGGCAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCCGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAA CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCACCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CGGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esco2 ( XM_031901549.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757409.1 Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X3, mRNA.

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CloneID	OXa57913
Clone ID Related Accession (Same CDS sequence)	XM_012964017.3 , XM_031901549.1
Accession Version	XM_031901549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	N-acetyltransferase ESCO2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
CCCACCCCAT CTCCCCGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTAG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCC 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCA TTACCGCCGC ACAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAAGGACCAT 
GCTGGCCAAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT CTCCTGTGAT AGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGTACCCCTG CGGGCAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCCGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAA CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCACCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CGGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757409.1
CDS	221..2473
Translation	MAALTPRKRKRSSVSSDGRQAFYGTPVKKLIVEYDDCPSPLTTRNSSRRNIRKSPPVTTYTNMADPTEKENVGASPLKSNAARKLVVSPIQTAPAPKAAYGNSPLTASPRAASPFKSSVSTRSFYKRDKMYVTPLDRKLINESKSSLSSGNESSVSSPTSPVATIAPRAKRNKKQPGKRKSGPKGKKTPPGSGKKAINVPARKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPEVEESAGVPSMKTTFLGLKMKPRPKLTVGAAFFATGKRPHSAPKRPSPNIKFPPLAKPPKQSTVPITKALPPHSALKGVVCVAEGQKQGQEGRKACNVPSESVAKKPESNKEVKLTKDHAGQESLPTSPAQAGTGMQRAAPDSPVIDEGEDSSVPKPSNKKGPTVFPIFSTPAGNKRPLDLRGDLASPVFSSTPTNAPAALRFPKQGKKKREGRKVAEDQLVIDAGQKHFGPIACNTCGMVYAAANLEDEAQHVQYHQRLLEGIRYVGWKKERVITEFWDGKIIMVSPDDPKYALKKAEEVRELVDSELGFQQVSLRCPSQTRTYMFVSNEKKIVGCLIAEPIREAYRVLAEPPSPHSVHREPLERHRAWRCSTEPEPAICGISRIWVFALMRRKAIASRLVDSVRSSFMYGSVLTTEEIAFSDPTPDGKLFASTYCKVPDFLVYNFVS

Target ORF information:

RefSeq Version	XM_031901549.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901549.1

ORF Insert Sequence:

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
CCCACCCCAT CTCCCCGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTAG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCC 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCA TTACCGCCGC ACAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAAGGACCAT 
GCTGGCCAAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT CTCCTGTGAT AGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGTACCCCTG CGGGCAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCCGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAA CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCACCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CGGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esco2 ( NM_001127990.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001121462.1 Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), mRNA.

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CloneID	OXa24555
Clone ID Related Accession (Same CDS sequence)	NM_001127990.1
Accession Version	NM_001127990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	N-acetyltransferase ESCO2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166227.1. ##Evidence-Data-START## Transcript exon combination :: BC166227.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCGTAT 
GGAAACTCTC CCCTTTTGGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGTTGATC 
AATGAGAGTA AATCCTCCTT ATCGAGTAGC AACGAAAGCT CAGTGTTTTC TCCTCCTACC 
AGCCCAGTGG CCACAATTGC CCCCAGGGCT AAACGGAACA AGAAGCAGCC TGGCAAGCGC 
AAGTCGGGCC CTAAAGGCAA GAAGACCCCA CCGGGCAGTG GAAAGAAGGC AATTAATGTG 
CCAGCCCGTA AGCCCACCCC ATCTCCCCGC AGTGACAAGG GGGCAGGAGT AAATACCCTT 
CCTTCTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGCCCA CCCCATCTCC CCGCAGTGAC AAGGGGGCAG GAGTAAATAC CCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
TCCACCCCAT CTCCCTGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTGG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCT 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCG TTACCGCCGC ATAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAGGGACCAT 
GCTGGCCGAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT TTCCTGTGAT GGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGCACCCCGG CGGGTAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCTGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAG CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCGCCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CAGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121462.1
CDS	36..2348
Translation	MAALTPRKRKRSSVSSDGRQAFYGTPVKKLIVEYDDCPSPLTTRNSSRRNIRKSPPVTTYTNMADPTEKENVGASPLKSNAARKLVVSPIQTAPAPKAAYGNSPLLASPRAASPFKSSVSTRSFYKRDKMYVTPLDRKLINESKSSLSSSNESSVFSPPTSPVATIAPRAKRNKKQPGKRKSGPKGKKTPPGSGKKAINVPARKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPEVEESAGVPSMKTTFLGLKMKPRPKLTVGAAFFATGKRPHSAPKRPSPNIKFPPLAKPPKQSTVPITKALPPHSALKGVVCVAEGQKQGQEGRKACNVPSESVAKKPESNKEVKLTRDHAGRESLPTSPAQAGTGMQRAAPDFPVMDEGEDSSVPKPSNKKGPTVFPIFSTPAGNKRPLDLRGDLASPVFSSTPTNAPAALRFPKQGKKKREGRKVAEDQLVIDAGQKHFGPIACSTCGMVYAAANLEDEAQHVQYHQRLLEGIRYVGWKKERVITEFWDGKIIMVSPDDPKYALKKAEEVRELVDSELGFQQVSLRCPSQTRTYMFVSNEKKIVGCLIAEPIREAYRVLAEPPSPHSVHREPLERHRAWRCSTEPEPAICGISRIWVFALMRRKAIASRLVDSVRSSFMYGSVLTTEEIAFSDPTPDGKLFASTYCKVPDFLVYNFVS

Target ORF information:

RefSeq Version	NM_001127990.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127990.1

ORF Insert Sequence:

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCGTAT 
GGAAACTCTC CCCTTTTGGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGTTGATC 
AATGAGAGTA AATCCTCCTT ATCGAGTAGC AACGAAAGCT CAGTGTTTTC TCCTCCTACC 
AGCCCAGTGG CCACAATTGC CCCCAGGGCT AAACGGAACA AGAAGCAGCC TGGCAAGCGC 
AAGTCGGGCC CTAAAGGCAA GAAGACCCCA CCGGGCAGTG GAAAGAAGGC AATTAATGTG 
CCAGCCCGTA AGCCCACCCC ATCTCCCCGC AGTGACAAGG GGGCAGGAGT AAATACCCTT 
CCTTCTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGCCCA CCCCATCTCC CCGCAGTGAC AAGGGGGCAG GAGTAAATAC CCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
TCCACCCCAT CTCCCTGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTGG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCT 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCG TTACCGCCGC ATAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAGGGACCAT 
GCTGGCCGAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT TTCCTGTGAT GGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGCACCCCGG CGGGTAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCTGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAG CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCGCCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CAGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esco2 ( XM_012964016.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819470.1 Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

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CloneID	OXa32184
Clone ID Related Accession (Same CDS sequence)	XM_012964016.2
Accession Version	XM_012964016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	N-acetyltransferase ESCO2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
TCCACCCCAT CTCCCTGCAG TGACAAGGGG GCAGGGGTAA ATACTCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCATCTCCCC GCAGTGACAA GGGGGCAGGG GTAAATACCC TTCCTTCTAA GCCCACCCCA 
TCTCCCCGCA GTGACAAGGG GGCAGGGGTA AATACCCTTC CTTCTAAGCC CACCCCAGAA 
GTAGAGGAAA GCGCCGGTGT CCCCAGCATG AAGACCACCT TTCTGGGGCT CAAGATGAAG 
CCGAGACCAA AACTCACGGT CGGAGCAGCA TTTTTTGCAA CGGGCAAGAG ACCCCACTCT 
GCTCCCAAGA GACCGTCCCC CAATATCAAG TTTCCCCCAT TGGCAAAGCC GCCGAAGCAG 
AGCACTGTAC CCATCACCAA AGCATTACCG CCGCACAGTG CTCTTAAAGG GGTTGTTTGT 
GTGGCTGAAG GGCAGAAGCA GGGGCAAGAA GGGAGGAAGG CGTGCAACGT ACCCTCTGAA 
TCTGTGGCCA AAAAGCCTGA AAGCAACAAG GAAGTCAAAC TGACGAAGGA CCATGCTGGC 
CAAGAGAGCC TACCTACCTC TCCAGCACAA GCAGGGACTG GCATGCAGCG AGCGGCGCCG 
GACTCTCCTG TGATAGACGA GGGTGAGGAC AGCTCTGTAC CAAAACCGTC CAATAAGAAA 
GGTCCAACTG TCTTTCCAAT CTTCAGTACC CCTGCGGGCA ATAAGAGACC TTTGGACCTG 
AGAGGAGACC TGGCCTCCCC TGTGTTCTCC AGCACCCCGA CTAATGCCCC CGCTGCCCTC 
AGGTTCCCCA AACAGGGCAA GAAGAAGAGA GAAGGAAGGA AGGTGGCAGA GGACCAGCTC 
GTCATTGATG CAGGACAGAA GCACTTTGGG CCAATAGCGT GCAACACGTG TGGGATGGTC 
TACGCTGCGG CCAATCTGGA GGATGAAGCT CAGCACGTCC AGTATCACCA GAGGCTTCTA 
GAGGGAATCC GATATGTGGG CTGGAAGAAA GAGCGAGTTA TTACCGAATT CTGGGATGGA 
AAAATCATCA TGGTTTCACC GGACGACCCT AAATACGCCC TGAAAAAGGC AGAGGAAGTT 
CGGGAGCTGG TGGATTCGGA GCTGGGATTT CAGCAGGTCT CCCTACGGTG CCCAAGCCAG 
ACCAGAACCT ACATGTTTGT GTCCAATGAG AAGAAGATTG TTGGCTGTTT GATTGCAGAG 
CCAATCAGAG AGGCATACAG AGTCCTCGCA GAACCCCCAT CTCCTCACTC GGTACATCGG 
GAACCTCTAG AACGCCATCG AGCTTGGAGG TGCTCAACCG AACCGGAACC TGCTATATGT 
GGTATCAGCA GGATTTGGGT GTTTGCCCTG ATGCGCAGAA AGGCCATCGC CAGCCGATTG 
GTGGATTCAG TCAGAAGTTC CTTTATGTAC GGCTCAGTCC TTACCACGGA AGAAATCGCC 
TTCTCCGACC CAACCCCCGA CGGCAAGCTG TTTGCTAGCA CCTACTGCAA GGTGCCGGAT 
TTCCTGGTCT ATAATTTTGT CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819470.1
CDS	228..2594
Translation	MAALTPRKRKRSSVSSDGRQAFYGTPVKKLIVEYDDCPSPLTTRNSSRRNIRKSPPVTTYTNMADPTEKENVGASPLKSNAARKLVVSPIQTAPAPKAAYGNSPLTASPRAASPFKSSVSTRSFYKRDKMYVTPLDRKLINESKSSLSSGNESSVSSPTSPVATIAPRAKRNKKQPGKRKSGPKGKKTPPGSGKKAINVPARKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPEVEESAGVPSMKTTFLGLKMKPRPKLTVGAAFFATGKRPHSAPKRPSPNIKFPPLAKPPKQSTVPITKALPPHSALKGVVCVAEGQKQGQEGRKACNVPSESVAKKPESNKEVKLTKDHAGQESLPTSPAQAGTGMQRAAPDSPVIDEGEDSSVPKPSNKKGPTVFPIFSTPAGNKRPLDLRGDLASPVFSSTPTNAPAALRFPKQGKKKREGRKVAEDQLVIDAGQKHFGPIACNTCGMVYAAANLEDEAQHVQYHQRLLEGIRYVGWKKERVITEFWDGKIIMVSPDDPKYALKKAEEVRELVDSELGFQQVSLRCPSQTRTYMFVSNEKKIVGCLIAEPIREAYRVLAEPPSPHSVHREPLERHRAWRCSTEPEPAICGISRIWVFALMRRKAIASRLVDSVRSSFMYGSVLTTEEIAFSDPTPDGKLFASTYCKVPDFLVYNFVS

Target ORF information:

RefSeq Version	XM_012964016.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964016.2

ORF Insert Sequence:

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
TCCACCCCAT CTCCCTGCAG TGACAAGGGG GCAGGGGTAA ATACTCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCATCTCCCC GCAGTGACAA GGGGGCAGGG GTAAATACCC TTCCTTCTAA GCCCACCCCA 
TCTCCCCGCA GTGACAAGGG GGCAGGGGTA AATACCCTTC CTTCTAAGCC CACCCCAGAA 
GTAGAGGAAA GCGCCGGTGT CCCCAGCATG AAGACCACCT TTCTGGGGCT CAAGATGAAG 
CCGAGACCAA AACTCACGGT CGGAGCAGCA TTTTTTGCAA CGGGCAAGAG ACCCCACTCT 
GCTCCCAAGA GACCGTCCCC CAATATCAAG TTTCCCCCAT TGGCAAAGCC GCCGAAGCAG 
AGCACTGTAC CCATCACCAA AGCATTACCG CCGCACAGTG CTCTTAAAGG GGTTGTTTGT 
GTGGCTGAAG GGCAGAAGCA GGGGCAAGAA GGGAGGAAGG CGTGCAACGT ACCCTCTGAA 
TCTGTGGCCA AAAAGCCTGA AAGCAACAAG GAAGTCAAAC TGACGAAGGA CCATGCTGGC 
CAAGAGAGCC TACCTACCTC TCCAGCACAA GCAGGGACTG GCATGCAGCG AGCGGCGCCG 
GACTCTCCTG TGATAGACGA GGGTGAGGAC AGCTCTGTAC CAAAACCGTC CAATAAGAAA 
GGTCCAACTG TCTTTCCAAT CTTCAGTACC CCTGCGGGCA ATAAGAGACC TTTGGACCTG 
AGAGGAGACC TGGCCTCCCC TGTGTTCTCC AGCACCCCGA CTAATGCCCC CGCTGCCCTC 
AGGTTCCCCA AACAGGGCAA GAAGAAGAGA GAAGGAAGGA AGGTGGCAGA GGACCAGCTC 
GTCATTGATG CAGGACAGAA GCACTTTGGG CCAATAGCGT GCAACACGTG TGGGATGGTC 
TACGCTGCGG CCAATCTGGA GGATGAAGCT CAGCACGTCC AGTATCACCA GAGGCTTCTA 
GAGGGAATCC GATATGTGGG CTGGAAGAAA GAGCGAGTTA TTACCGAATT CTGGGATGGA 
AAAATCATCA TGGTTTCACC GGACGACCCT AAATACGCCC TGAAAAAGGC AGAGGAAGTT 
CGGGAGCTGG TGGATTCGGA GCTGGGATTT CAGCAGGTCT CCCTACGGTG CCCAAGCCAG 
ACCAGAACCT ACATGTTTGT GTCCAATGAG AAGAAGATTG TTGGCTGTTT GATTGCAGAG 
CCAATCAGAG AGGCATACAG AGTCCTCGCA GAACCCCCAT CTCCTCACTC GGTACATCGG 
GAACCTCTAG AACGCCATCG AGCTTGGAGG TGCTCAACCG AACCGGAACC TGCTATATGT 
GGTATCAGCA GGATTTGGGT GTTTGCCCTG ATGCGCAGAA AGGCCATCGC CAGCCGATTG 
GTGGATTCAG TCAGAAGTTC CTTTATGTAC GGCTCAGTCC TTACCACGGA AGAAATCGCC 
TTCTCCGACC CAACCCCCGA CGGCAAGCTG TTTGCTAGCA CCTACTGCAA GGTGCCGGAT 
TTCCTGGTCT ATAATTTTGT CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esco2 ( XM_012964017.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819471.1 Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X2, mRNA.

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CloneID	OXa32185
Clone ID Related Accession (Same CDS sequence)	XM_012964017.2
Accession Version	XM_012964017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	N-acetyltransferase ESCO2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
CCCACCCCAT CTCCCCGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTAG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCC 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCA TTACCGCCGC ACAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAAGGACCAT 
GCTGGCCAAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT CTCCTGTGAT AGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGTACCCCTG CGGGCAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCCGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAA CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCACCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CAGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819471.1
CDS	230..2482
Translation	MAALTPRKRKRSSVSSDGRQAFYGTPVKKLIVEYDDCPSPLTTRNSSRRNIRKSPPVTTYTNMADPTEKENVGASPLKSNAARKLVVSPIQTAPAPKAAYGNSPLTASPRAASPFKSSVSTRSFYKRDKMYVTPLDRKLINESKSSLSSGNESSVSSPTSPVATIAPRAKRNKKQPGKRKSGPKGKKTPPGSGKKAINVPARKPTPSPRSDKGAGVNTLPSKSTPSPCSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPSPRSDKGAGVNTLPSKPTPEVEESAGVPSMKTTFLGLKMKPRPKLTVGAAFFATGKRPHSAPKRPSPNIKFPPLAKPPKQSTVPITKALPPHSALKGVVCVAEGQKQGQEGRKACNVPSESVAKKPESNKEVKLTKDHAGQESLPTSPAQAGTGMQRAAPDSPVIDEGEDSSVPKPSNKKGPTVFPIFSTPAGNKRPLDLRGDLASPVFSSTPTNAPAALRFPKQGKKKREGRKVAEDQLVIDAGQKHFGPIACNTCGMVYAAANLEDEAQHVQYHQRLLEGIRYVGWKKERVITEFWDGKIIMVSPDDPKYALKKAEEVRELVDSELGFQQVSLRCPSQTRTYMFVSNEKKIVGCLIAEPIREAYRVLAEPPSPHSVHREPLERHRAWRCSTEPEPAICGISRIWVFALMRRKAIASRLVDSVRSSFMYGSVLTTEEIAFSDPTPDGKLFASTYCKVPDFLVYNFVS

Target ORF information:

RefSeq Version	XM_012964017.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis establishment of sister chromatid cohesion N-acetyltransferase 2 (esco2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964017.2

ORF Insert Sequence:

ATGGCGGCCC TCACTCCCCG CAAGAGAAAA CGCAGCTCTG TCAGCTCGGA CGGGCGCCAG 
GCTTTCTATG GGACGCCAGT GAAGAAGCTG ATTGTTGAGT ACGACGACTG CCCGTCGCCC 
CTGACAACAC GGAACTCCAG TCGGCGTAAT ATAAGGAAGA GCCCCCCTGT GACCACATAC 
ACAAACATGG CCGACCCTAC AGAGAAGGAG AACGTGGGGG CTTCTCCATT AAAGTCCAAC 
GCCGCCCGGA AGCTGGTGGT CTCGCCCATA CAAACTGCGC CAGCGCCAAA AGCTGCATAC 
GGAAACTCTC CCCTTACCGC TTCACCGCGG GCTGCGTCCC CATTCAAGTC TTCCGTTTCT 
ACGCGCTCCT TCTACAAGCG CGACAAGATG TACGTCACCC CTCTGGACAG GAAGCTGATC 
AATGAGAGTA AATCCTCCTT ATCAAGCGGC AACGAAAGCT CCGTATCGTC ACCTACCAGC 
CCAGTGGCCA CAATTGCCCC CAGGGCTAAA CGGAACAAGA AGCAGCCTGG CAAGCGCAAG 
TCGGGCCCTA AAGGCAAGAA GACCCCACCG GGCAGTGGGA AGAAGGCAAT TAATGTGCCA 
GCCCGTAAGC CCACCCCATC TCCCCGCAGT GACAAGGGGG CAGGGGTAAA TACCCTTCCT 
TCTAAGTCCA CCCCATCTCC CTGCAGTGAC AAGGGGGCAG GGGTAAATAC TCTTCCTTCT 
AAGCCCACCC CATCTCCCCG CAGTGACAAG GGGGCAGGGG TAAATACCCT TCCTTCTAAG 
CCCACCCCAT CTCCCCGCAG TGACAAGGGG GCAGGGGTAA ATACCCTTCC TTCTAAGCCC 
ACCCCATCTC CCCGCAGTGA CAAGGGGGCA GGGGTAAATA CCCTTCCTTC TAAGCCCACC 
CCAGAAGTAG AGGAAAGCGC CGGTGTCCCC AGCATGAAGA CCACCTTTCT GGGGCTCAAG 
ATGAAGCCGA GACCAAAACT CACGGTCGGA GCAGCATTTT TTGCAACGGG CAAGAGACCC 
CACTCTGCTC CCAAGAGACC GTCCCCCAAT ATCAAGTTTC CCCCATTGGC AAAGCCGCCG 
AAGCAGAGCA CTGTACCCAT CACCAAAGCA TTACCGCCGC ACAGTGCTCT TAAAGGGGTT 
GTTTGTGTGG CTGAAGGGCA GAAGCAGGGG CAAGAAGGGA GGAAGGCGTG CAACGTACCC 
TCTGAATCTG TGGCCAAAAA GCCTGAAAGC AACAAGGAAG TCAAACTGAC GAAGGACCAT 
GCTGGCCAAG AGAGCCTACC TACCTCTCCA GCACAAGCAG GGACTGGCAT GCAGCGAGCG 
GCGCCGGACT CTCCTGTGAT AGACGAGGGT GAGGACAGCT CTGTACCAAA ACCGTCCAAT 
AAGAAAGGTC CAACTGTCTT TCCAATCTTC AGTACCCCTG CGGGCAATAA GAGACCTTTG 
GACCTGAGAG GAGACCTGGC CTCCCCTGTG TTCTCCAGCA CCCCGACTAA TGCCCCCGCT 
GCCCTCAGGT TCCCCAAACA GGGCAAGAAG AAGAGAGAAG GAAGGAAGGT GGCAGAGGAC 
CAGCTCGTCA TTGATGCAGG ACAGAAGCAC TTTGGGCCAA TAGCGTGCAA CACGTGTGGG 
ATGGTCTACG CTGCGGCCAA TCTGGAGGAT GAAGCTCAGC ACGTCCAGTA TCACCAGAGG 
CTTCTAGAGG GAATCCGATA TGTGGGCTGG AAGAAAGAGC GAGTTATTAC CGAATTCTGG 
GATGGAAAAA TCATCATGGT TTCACCGGAC GACCCTAAAT ACGCCCTGAA AAAGGCAGAG 
GAAGTTCGGG AGCTGGTGGA TTCGGAGCTG GGATTTCAGC AGGTCTCCCT ACGGTGCCCA 
AGCCAGACCA GAACCTACAT GTTTGTGTCC AATGAGAAGA AGATTGTTGG CTGTTTGATT 
GCAGAGCCAA TCAGAGAGGC ATACAGAGTC CTCGCAGAAC CCCCATCTCC TCACTCGGTA 
CATCGGGAAC CTCTAGAACG CCATCGAGCT TGGAGGTGCT CAACCGAACC GGAACCTGCT 
ATATGTGGTA TCAGCAGGAT TTGGGTGTTT GCCCTGATGC GCAGAAAGGC CATCGCCAGC 
CGATTGGTGG ATTCAGTCAG AAGTTCCTTT ATGTACGGCT CAGTCCTTAC CACGGAAGAA 
ATCGCCTTCT CCGACCCAAC CCCCGACGGC AAGCTGTTTG CTAGCACCTA CTGCAAGGTG 
CCGGATTTCC TGGTCTATAA TTTTGTCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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esco2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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