srsf5b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	srsf5b
Entrez Gene ID	436883
Full Name	serine/arginine-rich splicing factor 5b
Synonyms	sfrs5b,wu:fe15e05,zgc:113907,zgc:92278
General protein information	Preferred Names serine/arginine-rich splicing factor 5b Names splicing factor, arginine/serine-rich 5 splicing factor arginine/serine-rich 5 splicing factor, arginine/serine-rich 5b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160668.3	XP_005160725.1	splicing factor, arginine/serine-rich 5 isoform X2
NM_001002610.1	NP_001002610.1	splicing factor, arginine/serine-rich 5
XM_005160668.4	XP_005160725.1	splicing factor, arginine/serine-rich 5 isoform X2
XM_009294706.2	XP_009292981.1	splicing factor, arginine/serine-rich 5 isoform X1
XM_009294706.3	XP_009292981.1	splicing factor, arginine/serine-rich 5 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Joris M, Schloesser M, Baurain D, Hanikenne M, Muller M, Motte P
Nucleic acids research45(16)9547-9557(2017 Sep)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following srsf5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srsf5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24956	XM_005160668.4 Latest version!	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa37150	NM_001002610.1 Latest version!	Danio rerio serine and arginine rich splicing factor 5b (srsf5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
ODa41082	XM_009294706.3 Latest version!	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00
View Old Accession Versions >>
ODa24956	XM_005160668.3	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa41082	XM_009294706.2	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

srsf5b ( XM_005160668.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160725.1 Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24956
Clone ID Related Accession (Same CDS sequence)	XM_005160668.3 , XM_005160668.4
Accession Version	XM_005160668.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	splicing factor, arginine/serine-rich 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT GTCGTATTTT CATCGGTCGG TTAAACCCCT CTGCGCGGGA GAAGGACGTC 
GAGCGCTTCT TCAAGGGCTA CGGGAGAATC AGAGACATCG ACCTCAAACG AGGCTTCGGT 
TTTGTGGAAT TTGATGATCC AAGAGATGCT GAAGATGCTG TTTATGAGCT TGATGGAAAA 
GAGCTCTGCA ATGAGAGAGT GACCATTGAA CATGCACGCG TTCGTCTGCG TGGAGGCCGA 
GGTCGTGGGG GTGGAGGACG TTTCCCTGCT CGGTATGGTC GAGGCTCTCA GGACAGCCGA 
AGGAACCCTC CGCCAATGCG CACAGAGAAT CGCCTCATCG TGGAGAATTT ATCATCAAGA 
GTGAGCTGGC AGCCTGACTG TTGTGTCGTA TGGAAGAGCT CGCTAATGGT GGAGTTAAGC 
CCTACAGGGT CCCTCTCTCT GCTTAATCCA CTCCCATTGA GTCCCACGAC TAGTAGACTT 
CCGCCGATCG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160725.1
CDS	114..608
Translation	MSGCRIFIGRLNPSAREKDVERFFKGYGRIRDIDLKRGFGFVEFDDPRDAEDAVYELDGKELCNERVTIEHARVRLRGGRGRGGGGRFPARYGRGSQDSRRNPPPMRTENRLIVENLSSRVSWQPDCCVVWKSSLMVELSPTGSLSLLNPLPLSPTTSRLPPIA

Target ORF information:

RefSeq Version	XM_005160668.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160668.3

ORF Insert Sequence:

ATGAGCGGTT GTCGTATTTT CATCGGTCGG TTAAACCCCT CTGCGCGGGA GAAGGACGTC 
GAGCGCTTCT TCAAGGGCTA CGGGAGAATC AGAGACATCG ACCTCAAACG AGGCTTCGGT 
TTTGTGGAAT TTGATGATCC AAGAGATGCT GAAGATGCTG TTTATGAGCT TGATGGAAAA 
GAGCTCTGCA ATGAGAGAGT GACCATTGAA CATGCACGCG TTCGTCTGCG TGGAGGCCGA 
GGTCGTGGGG GTGGAGGACG TTTCCCTGCT CGGTATGGTC GAGGCTCTCA GGACAGCCGA 
AGGAACCCTC CGCCAATGCG CACAGAGAAT CGCCTCATCG TGGAGAATTT ATCATCAAGA 
GTGAGCTGGC AGCCTGACTG TTGTGTCGTA TGGAAGAGCT CGCTAATGGT GGAGTTAAGC 
CCTACAGGGT CCCTCTCTCT GCTTAATCCA CTCCCATTGA GTCCCACGAC TAGTAGACTT 
CCGCCGATCG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srsf5b ( XM_005160668.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160725.1 Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24956
Clone ID Related Accession (Same CDS sequence)	XM_005160668.3 , XM_005160668.4
Accession Version	XM_005160668.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	splicing factor, arginine/serine-rich 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160668.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT GTCGTATTTT CATCGGTCGG TTAAACCCCT CTGCGCGGGA GAAGGACGTC 
GAGCGCTTCT TCAAGGGCTA CGGGAGAATC AGAGACATCG ACCTCAAACG AGGCTTCGGT 
TTTGTGGAAT TTGATGATCC AAGAGATGCT GAAGATGCTG TTTATGAGCT TGATGGAAAA 
GAGCTCTGCA ATGAGAGAGT GACCATTGAA CATGCACGCG TTCGTCTGCG TGGAGGCCGA 
GGTCGTGGGG GTGGAGGACG TTTCCCTGCT CGGTATGGTC GAGGCTCTCA GGACAGCCGA 
AGGAACCCTC CGCCAATGCG CACAGAGAAT CGCCTCATCG TGGAGAATTT ATCATCAAGA 
GTGAGCTGGC AGCCTGACTG TTGTGTCGTA TGGAAGAGCT CGCTAATGGT GGAGTTAAGC 
CCTACAGGGT CCCTCTCTCT GCTTAATCCA CTCCCATTGA GTCCCACGAC TAGTAGACTT 
CCGCCGATCG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160725.1
CDS	162..656
Translation	MSGCRIFIGRLNPSAREKDVERFFKGYGRIRDIDLKRGFGFVEFDDPRDAEDAVYELDGKELCNERVTIEHARVRLRGGRGRGGGGRFPARYGRGSQDSRRNPPPMRTENRLIVENLSSRVSWQPDCCVVWKSSLMVELSPTGSLSLLNPLPLSPTTSRLPPIA

Target ORF information:

RefSeq Version	XM_005160668.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160668.4

ORF Insert Sequence:

ATGAGCGGTT GTCGTATTTT CATCGGTCGG TTAAACCCCT CTGCGCGGGA GAAGGACGTC 
GAGCGCTTCT TCAAGGGCTA CGGGAGAATC AGAGACATCG ACCTCAAACG AGGCTTCGGT 
TTTGTGGAAT TTGATGATCC AAGAGATGCT GAAGATGCTG TTTATGAGCT TGATGGAAAA 
GAGCTCTGCA ATGAGAGAGT GACCATTGAA CATGCACGCG TTCGTCTGCG TGGAGGCCGA 
GGTCGTGGGG GTGGAGGACG TTTCCCTGCT CGGTATGGTC GAGGCTCTCA GGACAGCCGA 
AGGAACCCTC CGCCAATGCG CACAGAGAAT CGCCTCATCG TGGAGAATTT ATCATCAAGA 
GTGAGCTGGC AGCCTGACTG TTGTGTCGTA TGGAAGAGCT CGCTAATGGT GGAGTTAAGC 
CCTACAGGGT CCCTCTCTCT GCTTAATCCA CTCCCATTGA GTCCCACGAC TAGTAGACTT 
CCGCCGATCG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srsf5b ( NM_001002610.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002610.1 Danio rerio serine and arginine rich splicing factor 5b (srsf5b), mRNA.

Price & Availability↑

CloneID	ODa37150
Clone ID Related Accession (Same CDS sequence)	NM_001002610.1
Accession Version	NM_001002610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	splicing factor, arginine/serine-rich 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC075982.1. On or before Jan 5, 2007 this sequence version replaced XM_703894.1, XM_682819.1, XM_703895.1, XM_703896.1, XM_703897.1, XM_703898.1, XM_703899.1. ##Evidence-Data-START## Transcript exon combination :: BC075982.1, CN318580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476770 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGGTT GTCGTATTTT CATCGGTCGG TTAAACCCCT CTGCGCGGGA GAAGGACGTC 
GAGCGCTTCT TCAAGGGCTA CGGGAGAATC AGAGACATCG ACCTCAAACG AGGCTTCGGT 
TTTGTGGAAT TTGATGATCC AAGAGATGCT GAAGATGCTG TTTATGAGCT TGATGGAAAA 
GAGCTCTGCA ATGAGAGAGT GACCATTGAA CATGCACGCG TTCGTCTGCG TGGAGGCCGA 
GGTCGTGGGG GTGGAGGACG TTTCCCTGCT CGGTATGGTC GAGGCTCTCA GGACAGCCGA 
AGGAACCCTC CGCCAATGCG CACAGAGAAT CGCCTCATCG TGGAGAATTT ATCATCAAGA 
GTGAGCTGGC AGGATTTAAA GGATTTCATG AGACAAGCTG GAGAGGTTAC TTTTGCAGAT 
GCCCATCGGC CAAATCTGAA TGAAGGCGTG GTTGAATTTG CTTCTCATAG TGATCTGAAA 
AATGCCCTGG AGAAGCTGTC TGGGAAGGAG ATCAATGGAA GGAAAATCAA ACTTGTTGAG 
GCGTCCAGGA AAAAATCCAG ATCTCGCTCT CGCTCGAACA GCTCATCCCG TTCTCGTTCC 
CGAGGCCGCT CTGCTTCCCG CTCCCGAAGC CGTTCTCGTT CTCACAGCCC TAAATCCCAC 
AACCGCTCCA GAAGTCGCTC CCGTTCAGCC AGCGCTTCTC CTGTGCGGCC CAAAGAAGCT 
CCTCGTCCTG AATCAGCATC TCCTCCAGCC TCAGCACAGC ATCCACCTCG TTCTCGCTCA 
CGATCCCGCT CCCGCTCTCG TTCCCGTTCT CGCTCCCGAT CCCGCTCCCG CTCCCCATCC 
ACTGACAGCC GCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002610.1
CDS	57..914
Translation	MSGCRIFIGRLNPSAREKDVERFFKGYGRIRDIDLKRGFGFVEFDDPRDAEDAVYELDGKELCNERVTIEHARVRLRGGRGRGGGGRFPARYGRGSQDSRRNPPPMRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPNLNEGVVEFASHSDLKNALEKLSGKEINGRKIKLVEASRKKSRSRSRSNSSSRSRSRGRSASRSRSRSRSHSPKSHNRSRSRSRSASASPVRPKEAPRPESASPPASAQHPPRSRSRSRSRSRSRSRSRSRSRSPSTDSRH

Target ORF information:

RefSeq Version	NM_001002610.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serine and arginine rich splicing factor 5b (srsf5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002610.1

ORF Insert Sequence:

ATGAGCGGTT GTCGTATTTT CATCGGTCGG TTAAACCCCT CTGCGCGGGA GAAGGACGTC 
GAGCGCTTCT TCAAGGGCTA CGGGAGAATC AGAGACATCG ACCTCAAACG AGGCTTCGGT 
TTTGTGGAAT TTGATGATCC AAGAGATGCT GAAGATGCTG TTTATGAGCT TGATGGAAAA 
GAGCTCTGCA ATGAGAGAGT GACCATTGAA CATGCACGCG TTCGTCTGCG TGGAGGCCGA 
GGTCGTGGGG GTGGAGGACG TTTCCCTGCT CGGTATGGTC GAGGCTCTCA GGACAGCCGA 
AGGAACCCTC CGCCAATGCG CACAGAGAAT CGCCTCATCG TGGAGAATTT ATCATCAAGA 
GTGAGCTGGC AGGATTTAAA GGATTTCATG AGACAAGCTG GAGAGGTTAC TTTTGCAGAT 
GCCCATCGGC CAAATCTGAA TGAAGGCGTG GTTGAATTTG CTTCTCATAG TGATCTGAAA 
AATGCCCTGG AGAAGCTGTC TGGGAAGGAG ATCAATGGAA GGAAAATCAA ACTTGTTGAG 
GCGTCCAGGA AAAAATCCAG ATCTCGCTCT CGCTCGAACA GCTCATCCCG TTCTCGTTCC 
CGAGGCCGCT CTGCTTCCCG CTCCCGAAGC CGTTCTCGTT CTCACAGCCC TAAATCCCAC 
AACCGCTCCA GAAGTCGCTC CCGTTCAGCC AGCGCTTCTC CTGTGCGGCC CAAAGAAGCT 
CCTCGTCCTG AATCAGCATC TCCTCCAGCC TCAGCACAGC ATCCACCTCG TTCTCGCTCA 
CGATCCCGCT CCCGCTCTCG TTCCCGTTCT CGCTCCCGAT CCCGCTCCCG CTCCCCATCC 
ACTGACAGCC GCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srsf5b ( XM_009294706.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292981.1 Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41082
Clone ID Related Accession (Same CDS sequence)	XM_009294706.2 , XM_009294706.3
Accession Version	XM_009294706.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	splicing factor, arginine/serine-rich 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAA AGCCTGTGCT CTGCCACACT GTAATCACTT GTGTTTTGGA TTTAAAGGAT 
TTCATGAGAC AAGCTGGAGA GGTTACTTTT GCAGATGCCC ATCGGCCAAA TCTGAATGAA 
GGCGTGGTTG AATTTGCTTC TCATAGTGAT CTGAAAAATG CCCTGGAGAA GCTGTCTGGG 
AAGGAGATCA ATGGAAGGAA AATCAAACTT GTTGAGGCGT CCAGGAAAAA ATCCAGATCT 
CGCTCTCGCT CGAACAGCTC ATCCCGTTCT CGTTCCCGAG GCCGCTCTGC TTCCCGCTCC 
CGAAGCCGTT CTCGTTCTCA CAGCCCTAAA TCCCACAACC GCTCCAGAAG TCGCTCCCGT 
TCAGCCAGCG CTTCTCCTGT GCGGCCCAAA GAAGCTCCTC GTCCTGAATC AGCATCTCCT 
CCAGCCTCAG CACAGCATCC ACCTCGTTCT CGCTCACGAT CCCGCTCCCG CTCTCGTTCC 
CGTTCTCGCT CCCGATCCCG CTCCCGCTCC CCATCCACTG ACAGCCGCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292981.1
CDS	365..898
Translation	MEGKPVLCHTVITCVLDLKDFMRQAGEVTFADAHRPNLNEGVVEFASHSDLKNALEKLSGKEINGRKIKLVEASRKKSRSRSRSNSSSRSRSRGRSASRSRSRSRSHSPKSHNRSRSRSRSASASPVRPKEAPRPESASPPASAQHPPRSRSRSRSRSRSRSRSRSRSRSPSTDSRH

Target ORF information:

RefSeq Version	XM_009294706.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294706.2

ORF Insert Sequence:

ATGGAAGGAA AGCCTGTGCT CTGCCACACT GTAATCACTT GTGTTTTGGA TTTAAAGGAT 
TTCATGAGAC AAGCTGGAGA GGTTACTTTT GCAGATGCCC ATCGGCCAAA TCTGAATGAA 
GGCGTGGTTG AATTTGCTTC TCATAGTGAT CTGAAAAATG CCCTGGAGAA GCTGTCTGGG 
AAGGAGATCA ATGGAAGGAA AATCAAACTT GTTGAGGCGT CCAGGAAAAA ATCCAGATCT 
CGCTCTCGCT CGAACAGCTC ATCCCGTTCT CGTTCCCGAG GCCGCTCTGC TTCCCGCTCC 
CGAAGCCGTT CTCGTTCTCA CAGCCCTAAA TCCCACAACC GCTCCAGAAG TCGCTCCCGT 
TCAGCCAGCG CTTCTCCTGT GCGGCCCAAA GAAGCTCCTC GTCCTGAATC AGCATCTCCT 
CCAGCCTCAG CACAGCATCC ACCTCGTTCT CGCTCACGAT CCCGCTCCCG CTCTCGTTCC 
CGTTCTCGCT CCCGATCCCG CTCCCGCTCC CCATCCACTG ACAGCCGCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srsf5b ( XM_009294706.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292981.1 Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41082
Clone ID Related Accession (Same CDS sequence)	XM_009294706.2 , XM_009294706.3
Accession Version	XM_009294706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	splicing factor, arginine/serine-rich 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAA AGCCTGTGCT CTGCCACACT GTAATCACTT GTGTTTTGGA TTTAAAGGAT 
TTCATGAGAC AAGCTGGAGA GGTTACTTTT GCAGATGCCC ATCGGCCAAA TCTGAATGAA 
GGCGTGGTTG AATTTGCTTC TCATAGTGAT CTGAAAAATG CCCTGGAGAA GCTGTCTGGG 
AAGGAGATCA ATGGAAGGAA AATCAAACTT GTTGAGGCGT CCAGGAAAAA ATCCAGATCT 
CGCTCTCGCT CGAACAGCTC ATCCCGTTCT CGTTCCCGAG GCCGCTCTGC TTCCCGCTCC 
CGAAGCCGTT CTCGTTCTCA CAGCCCTAAA TCCCACAACC GCTCCAGAAG TCGCTCCCGT 
TCAGCCAGCG CTTCTCCTGT GCGGCCCAAA GAAGCTCCTC GTCCTGAATC AGCATCTCCT 
CCAGCCTCAG CACAGCATCC ACCTCGTTCT CGCTCACGAT CCCGCTCCCG CTCTCGTTCC 
CGTTCTCGCT CCCGATCCCG CTCCCGCTCC CCATCCACTG ACAGCCGCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292981.1
CDS	371..904
Translation	MEGKPVLCHTVITCVLDLKDFMRQAGEVTFADAHRPNLNEGVVEFASHSDLKNALEKLSGKEINGRKIKLVEASRKKSRSRSRSNSSSRSRSRGRSASRSRSRSRSHSPKSHNRSRSRSRSASASPVRPKEAPRPESASPPASAQHPPRSRSRSRSRSRSRSRSRSRSRSPSTDSRH

Target ORF information:

RefSeq Version	XM_009294706.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serine/arginine-rich splicing factor 5b (srsf5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294706.3

ORF Insert Sequence:

ATGGAAGGAA AGCCTGTGCT CTGCCACACT GTAATCACTT GTGTTTTGGA TTTAAAGGAT 
TTCATGAGAC AAGCTGGAGA GGTTACTTTT GCAGATGCCC ATCGGCCAAA TCTGAATGAA 
GGCGTGGTTG AATTTGCTTC TCATAGTGAT CTGAAAAATG CCCTGGAGAA GCTGTCTGGG 
AAGGAGATCA ATGGAAGGAA AATCAAACTT GTTGAGGCGT CCAGGAAAAA ATCCAGATCT 
CGCTCTCGCT CGAACAGCTC ATCCCGTTCT CGTTCCCGAG GCCGCTCTGC TTCCCGCTCC 
CGAAGCCGTT CTCGTTCTCA CAGCCCTAAA TCCCACAACC GCTCCAGAAG TCGCTCCCGT 
TCAGCCAGCG CTTCTCCTGT GCGGCCCAAA GAAGCTCCTC GTCCTGAATC AGCATCTCCT 
CCAGCCTCAG CACAGCATCC ACCTCGTTCT CGCTCACGAT CCCGCTCCCG CTCTCGTTCC 
CGTTCTCGCT CCCGATCCCG CTCCCGCTCC CCATCCACTG ACAGCCGCCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Nucleic acids research45(16)9547-9557(2017 Sep)
Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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srsf5b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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