fam53b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fam53b
Entrez Gene ID	406586
Full Name	family with sequence similarity 53, member B
Synonyms	wu:fo86c04,zgc:55329,zgc:77156
General protein information	Preferred Names family with sequence similarity 53, member B Names protein FAM53B protein simplet simplet smp
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156440.3	XP_005156497.1	protein FAM53B isoform X1
XM_005156440.4	XP_005156497.1	protein FAM53B isoform X1
NM_001007187.1	NP_001007188.1	protein FAM53B

Pathways from BioSystems

Homologies

	FAM53B	XP_001083541.1
Gallus gallus (chicken)	FAM53B	XP_001231634.3
Homo sapiens (human)	FAM53B	NP_055476.3
Canis lupus familiaris (dog)	FAM53B	XP_005637889.1
Bos taurus (cattle)	FAM53B	XP_005225919.1
Rattus norvegicus (Norway rat)	Fam53b	NP_001101026.1
Pan troglodytes (chimpanzee)	FAM53B	XP_001135534.2
Mus musculus (house mouse)	Fam53b	NP_780477.2
Danio rerio (zebrafish)	fam53b	NP_001007188.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Simplet/Fam53b is required for Wnt signal transduction by regulating ?-catenin nuclear localization.
Kizil C, K?chler B, Yan JJ, ?zhan G, Moro E, Argenton F, Brand M, Weidinger G, Antos CL
Development (Cambridge, England)141(18)3529-39(2014 Sep)

Identification of possible downstream genes required for the extension of peripheral axons in primary sensory neurons.
Aoki M, Segawa H, Naito M, Okamoto H
Biochemical and biophysical research communications445(2)357-62(2014 Mar)

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Aanes H, Winata CL, Lin CH, Chen JP, Srinivasan KG, Lee SG, Lim AY, Hajan HS, Collas P, Bourque G, Gong Z, Korzh V, Alestr?m P, Mathavan S
Genome research21(8)1328-38(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

smp is required in fin regeneration through control of cell proliferation, the regulation of specific genes and proper bone patterning.
Title: Simplet controls cell proliferation and gene transcription during zebrafish caudal fin regeneration.

The following fam53b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fam53b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34878	NM_001007187.1 Latest version!	Danio rerio family with sequence similarity 53 member B (fam53b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
ODa18271	XM_005156440.4 Latest version!	Danio rerio family with sequence similarity 53, member B (fam53b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
ODa18271	XM_005156440.3	Danio rerio family with sequence similarity 53, member B (fam53b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fam53b ( NM_001007187.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007188.1 Danio rerio family with sequence similarity 53 member B (fam53b), mRNA.

Price & Availability↑

CloneID	ODa34878
Clone ID Related Accession (Same CDS sequence)	NM_001007187.1
Accession Version	NM_001007187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	protein FAM53B
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC066405.1. ##Evidence-Data-START## Transcript exon combination :: BC066405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGTTG CCATGGTAAT TATTCACACT AAAACACTGG AAAAGAAGGC AGTTGACGAT 
GTAACATCCG AAGCGTCTCT GCAGCCGCAG GAGCCGCTGA CCATGAGCCA GGGGACAGCT 
CTGTTCTCCT GTGGAATCAT GGACTCTGGG CGCTGGGCAG ATGTTGGCAG TGTGTGTGGC 
GTCCAGCAGC GGCCTGTGGG CTCCAGTCTG GAGAGCCTGT GGGACAGCAT GCGTGAGGCG 
GGCGCCACTG GCGGCATCAG TGGGTTACTG CGGGATCTGA GTCTGAGCGA AGCGTCTCCT 
GCCAGTGCTG CGCCGCCCAG CAAACGCCAG TGCCGCTCGC TGTCCTGCTC GGATGAGCTG 
GGCTGCCGCT CATCCTGGCG TCCGCAGGGT TCGCGCGTCT GGACAGCGGT GGAAAAGCGG 
CGCTGCCACA GCGGAGGGAG CGTCCAGAGA GGAGTCTTCA ATCCATCAGG CTTTCCTGCA 
ATGCAGCGCA GCTCCAGCTT CAGTCTGCCG GCGCACAGCA GTCATCTGGA GCCCTTCACT 
CACGGTTTCC CATTCCAGGC ATTCTCCGAG TGTCCGCAGA CGCCGCAGAC GCTGTACCGT 
TCACATGAGC AGATCTGCCC GGCGGAGGCC AGCTCCCCTG AATCCACCCC AGAGCTCCAG 
CGCCGCAGCG GGCAGACTGG ACTCGCACGC AGCCGATCAC AGCCCTGCGT ACACAACCAT 
CAGAAGATCG GCGTCAAGCG CAGGAGACCG GCCGACTCGC ACAAACAGAG GCCCTCGCTG 
GACCTGCTCA AGATGACACA GAAGCTGCAG GATTTTCACA GTCTCAGCTG TCCAGGATTC 
AGCGGCGACG ACAAAACTCT GCCGTCCAGC AGCCCGGCAC TCCTCAACGA CACATGCGAG 
CGTGCTCAGA ATGACAGCTC AGCGGATGCC CCCATCCACC AATCAGAGAG CAGCTCAGAG 
GACGCCCTCA TCCACCAATC AGATAGCAGC TCAGCGGACG CCCTCATCCA CCAATCAGAA 
AGCAGCAGGC CCGCAGGAAA GGAGCGCGAA TGCTTGTGGG CGGGGCTTTG TAGCAGAAGG 
GGCCGGGACT TATTCCAGCT GGGCGGAGAA CTGGATATCG AGCAGATTGA GAGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007188.1
CDS	184..1323
Translation	MCVAMVIIHTKTLEKKAVDDVTSEASLQPQEPLTMSQGTALFSCGIMDSGRWADVGSVCGVQQRPVGSSLESLWDSMREAGATGGISGLLRDLSLSEASPASAAPPSKRQCRSLSCSDELGCRSSWRPQGSRVWTAVEKRRCHSGGSVQRGVFNPSGFPAMQRSSSFSLPAHSSHLEPFTHGFPFQAFSECPQTPQTLYRSHEQICPAEASSPESTPELQRRSGQTGLARSRSQPCVHNHQKIGVKRRRPADSHKQRPSLDLLKMTQKLQDFHSLSCPGFSGDDKTLPSSSPALLNDTCERAQNDSSADAPIHQSESSSEDALIHQSDSSSADALIHQSESSRPAGKERECLWAGLCSRRGRDLFQLGGELDIEQIERN

Target ORF information:

RefSeq Version	NM_001007187.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 53 member B (fam53b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007187.1

ORF Insert Sequence:

ATGTGTGTTG CCATGGTAAT TATTCACACT AAAACACTGG AAAAGAAGGC AGTTGACGAT 
GTAACATCCG AAGCGTCTCT GCAGCCGCAG GAGCCGCTGA CCATGAGCCA GGGGACAGCT 
CTGTTCTCCT GTGGAATCAT GGACTCTGGG CGCTGGGCAG ATGTTGGCAG TGTGTGTGGC 
GTCCAGCAGC GGCCTGTGGG CTCCAGTCTG GAGAGCCTGT GGGACAGCAT GCGTGAGGCG 
GGCGCCACTG GCGGCATCAG TGGGTTACTG CGGGATCTGA GTCTGAGCGA AGCGTCTCCT 
GCCAGTGCTG CGCCGCCCAG CAAACGCCAG TGCCGCTCGC TGTCCTGCTC GGATGAGCTG 
GGCTGCCGCT CATCCTGGCG TCCGCAGGGT TCGCGCGTCT GGACAGCGGT GGAAAAGCGG 
CGCTGCCACA GCGGAGGGAG CGTCCAGAGA GGAGTCTTCA ATCCATCAGG CTTTCCTGCA 
ATGCAGCGCA GCTCCAGCTT CAGTCTGCCG GCGCACAGCA GTCATCTGGA GCCCTTCACT 
CACGGTTTCC CATTCCAGGC ATTCTCCGAG TGTCCGCAGA CGCCGCAGAC GCTGTACCGT 
TCACATGAGC AGATCTGCCC GGCGGAGGCC AGCTCCCCTG AATCCACCCC AGAGCTCCAG 
CGCCGCAGCG GGCAGACTGG ACTCGCACGC AGCCGATCAC AGCCCTGCGT ACACAACCAT 
CAGAAGATCG GCGTCAAGCG CAGGAGACCG GCCGACTCGC ACAAACAGAG GCCCTCGCTG 
GACCTGCTCA AGATGACACA GAAGCTGCAG GATTTTCACA GTCTCAGCTG TCCAGGATTC 
AGCGGCGACG ACAAAACTCT GCCGTCCAGC AGCCCGGCAC TCCTCAACGA CACATGCGAG 
CGTGCTCAGA ATGACAGCTC AGCGGATGCC CCCATCCACC AATCAGAGAG CAGCTCAGAG 
GACGCCCTCA TCCACCAATC AGATAGCAGC TCAGCGGACG CCCTCATCCA CCAATCAGAA 
AGCAGCAGGC CCGCAGGAAA GGAGCGCGAA TGCTTGTGGG CGGGGCTTTG TAGCAGAAGG 
GGCCGGGACT TATTCCAGCT GGGCGGAGAA CTGGATATCG AGCAGATTGA GAGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam53b ( XM_005156440.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156497.1 Danio rerio family with sequence similarity 53, member B (fam53b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18271
Clone ID Related Accession (Same CDS sequence)	XM_005156440.3 , XM_005156440.4
Accession Version	XM_005156440.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM53B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTG CCATGGTAAT TATTCACACT AAAACACTGG AAAAGAAGGC GGTTGACGAT 
GTAACATCCG AAGCGTCTCT GCAGCCGGAG CCGCTGACCA TGAGCCAGGG GACAGCTCTG 
TTCTCCTGTG GAATCATGGA CTCTGGGCGC TGGGCAGATG TTGGGAGTGT GTGTGGCGTC 
CAGCAGCGGC CTGTGGGCTC CAGTCTGGAG AGCCTGTGGG ACAGCATGCG TGAGGCGGGC 
GCCACTGGCG GCATCAGCGG GTTACTGCGG GATCTGAGTC TGAGCGAAGC GTCTCCTGCC 
AGTGCTGCGC CGCCCAGCAA ACGCCAGTGC CGCTCGCTGT CCTGCTCGGA TGAGCTGGGC 
TGCCGCTCAT CCTGGCGTCC GCAGGGTTCG CGCGTCTGGA CAACGGTGGA AAAGCGGCGC 
TGCCACAGTG GAGGGAGCGT CCAGAGAGGA GTCTTCAATC CATCAGGCTT TCCTGCAATG 
CAGCGCAGCT CCAGCTTCAG TCTGCCGGCG CACAGCAGTC ATCTGGAGCC CTTCACTCAC 
GGTTTCCCAT TCCAGGCATT CTCCGAGTGT CCGCAGACGC CGCAGACGCT GTACCGTTCA 
CATGAGCAGA TCTGCCCGGC GGAGGCCAGC TCCCCTGAAT CCACCCCAGA GCTCCAGCGC 
CGCAGCGGGC AGAGCGGACT CGCACGCAGC CGATCACAGC CCTGCGTACA CAACCATCAG 
AAGATCGGCG TCAAGCGCAG GAGACCGGCC GACTCGCACA AACAGAGGCC CTCGCTGGAC 
CTGCTCAAGA TGACACAGAA GCTGCAGGAT TTTCACAGTC TCAGCTGTCC AGGATTCAGC 
GGCGACGACA AAACTCTGCC GTCCAGCAGC CCGGCACTCC TCAACGACAC ATGTGAGCGT 
GCTCAGAATG ACAGCTCAGC GGATGCCCCC ATCCACCAAT CAGAGAGCAG CTCAGAGGAC 
GCCCTCATCC ACCAATCAGA TAGCAGCTCA GCGGACGCCC TCATCCACCA ATCAGAAAGC 
AGCAGGCCCG CAGGAAAGGA GCGCGAATGC TTGTGGGCGG GGCTTTGTAG CAGAAGGGGC 
CGGGACTTAT TCCAGCTGGG CGGAGAACTG GATATCGAGC AGATTGAGAG GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156497.1
CDS	191..1327
Translation	MCVAMVIIHTKTLEKKAVDDVTSEASLQPEPLTMSQGTALFSCGIMDSGRWADVGSVCGVQQRPVGSSLESLWDSMREAGATGGISGLLRDLSLSEASPASAAPPSKRQCRSLSCSDELGCRSSWRPQGSRVWTTVEKRRCHSGGSVQRGVFNPSGFPAMQRSSSFSLPAHSSHLEPFTHGFPFQAFSECPQTPQTLYRSHEQICPAEASSPESTPELQRRSGQSGLARSRSQPCVHNHQKIGVKRRRPADSHKQRPSLDLLKMTQKLQDFHSLSCPGFSGDDKTLPSSSPALLNDTCERAQNDSSADAPIHQSESSSEDALIHQSDSSSADALIHQSESSRPAGKERECLWAGLCSRRGRDLFQLGGELDIEQIERN

Target ORF information:

RefSeq Version	XM_005156440.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 53, member B (fam53b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156440.3

ORF Insert Sequence:

ATGTGTGTTG CCATGGTAAT TATTCACACT AAAACACTGG AAAAGAAGGC GGTTGACGAT 
GTAACATCCG AAGCGTCTCT GCAGCCGGAG CCGCTGACCA TGAGCCAGGG GACAGCTCTG 
TTCTCCTGTG GAATCATGGA CTCTGGGCGC TGGGCAGATG TTGGGAGTGT GTGTGGCGTC 
CAGCAGCGGC CTGTGGGCTC CAGTCTGGAG AGCCTGTGGG ACAGCATGCG TGAGGCGGGC 
GCCACTGGCG GCATCAGCGG GTTACTGCGG GATCTGAGTC TGAGCGAAGC GTCTCCTGCC 
AGTGCTGCGC CGCCCAGCAA ACGCCAGTGC CGCTCGCTGT CCTGCTCGGA TGAGCTGGGC 
TGCCGCTCAT CCTGGCGTCC GCAGGGTTCG CGCGTCTGGA CAACGGTGGA AAAGCGGCGC 
TGCCACAGTG GAGGGAGCGT CCAGAGAGGA GTCTTCAATC CATCAGGCTT TCCTGCAATG 
CAGCGCAGCT CCAGCTTCAG TCTGCCGGCG CACAGCAGTC ATCTGGAGCC CTTCACTCAC 
GGTTTCCCAT TCCAGGCATT CTCCGAGTGT CCGCAGACGC CGCAGACGCT GTACCGTTCA 
CATGAGCAGA TCTGCCCGGC GGAGGCCAGC TCCCCTGAAT CCACCCCAGA GCTCCAGCGC 
CGCAGCGGGC AGAGCGGACT CGCACGCAGC CGATCACAGC CCTGCGTACA CAACCATCAG 
AAGATCGGCG TCAAGCGCAG GAGACCGGCC GACTCGCACA AACAGAGGCC CTCGCTGGAC 
CTGCTCAAGA TGACACAGAA GCTGCAGGAT TTTCACAGTC TCAGCTGTCC AGGATTCAGC 
GGCGACGACA AAACTCTGCC GTCCAGCAGC CCGGCACTCC TCAACGACAC ATGTGAGCGT 
GCTCAGAATG ACAGCTCAGC GGATGCCCCC ATCCACCAAT CAGAGAGCAG CTCAGAGGAC 
GCCCTCATCC ACCAATCAGA TAGCAGCTCA GCGGACGCCC TCATCCACCA ATCAGAAAGC 
AGCAGGCCCG CAGGAAAGGA GCGCGAATGC TTGTGGGCGG GGCTTTGTAG CAGAAGGGGC 
CGGGACTTAT TCCAGCTGGG CGGAGAACTG GATATCGAGC AGATTGAGAG GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam53b ( XM_005156440.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156497.1 Danio rerio family with sequence similarity 53, member B (fam53b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18271
Clone ID Related Accession (Same CDS sequence)	XM_005156440.3 , XM_005156440.4
Accession Version	XM_005156440.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM53B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156440.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTG CCATGGTAAT TATTCACACT AAAACACTGG AAAAGAAGGC GGTTGACGAT 
GTAACATCCG AAGCGTCTCT GCAGCCGGAG CCGCTGACCA TGAGCCAGGG GACAGCTCTG 
TTCTCCTGTG GAATCATGGA CTCTGGGCGC TGGGCAGATG TTGGGAGTGT GTGTGGCGTC 
CAGCAGCGGC CTGTGGGCTC CAGTCTGGAG AGCCTGTGGG ACAGCATGCG TGAGGCGGGC 
GCCACTGGCG GCATCAGCGG GTTACTGCGG GATCTGAGTC TGAGCGAAGC GTCTCCTGCC 
AGTGCTGCGC CGCCCAGCAA ACGCCAGTGC CGCTCGCTGT CCTGCTCGGA TGAGCTGGGC 
TGCCGCTCAT CCTGGCGTCC GCAGGGTTCG CGCGTCTGGA CAACGGTGGA AAAGCGGCGC 
TGCCACAGTG GAGGGAGCGT CCAGAGAGGA GTCTTCAATC CATCAGGCTT TCCTGCAATG 
CAGCGCAGCT CCAGCTTCAG TCTGCCGGCG CACAGCAGTC ATCTGGAGCC CTTCACTCAC 
GGTTTCCCAT TCCAGGCATT CTCCGAGTGT CCGCAGACGC CGCAGACGCT GTACCGTTCA 
CATGAGCAGA TCTGCCCGGC GGAGGCCAGC TCCCCTGAAT CCACCCCAGA GCTCCAGCGC 
CGCAGCGGGC AGAGCGGACT CGCACGCAGC CGATCACAGC CCTGCGTACA CAACCATCAG 
AAGATCGGCG TCAAGCGCAG GAGACCGGCC GACTCGCACA AACAGAGGCC CTCGCTGGAC 
CTGCTCAAGA TGACACAGAA GCTGCAGGAT TTTCACAGTC TCAGCTGTCC AGGATTCAGC 
GGCGACGACA AAACTCTGCC GTCCAGCAGC CCGGCACTCC TCAACGACAC ATGTGAGCGT 
GCTCAGAATG ACAGCTCAGC GGATGCCCCC ATCCACCAAT CAGAGAGCAG CTCAGAGGAC 
GCCCTCATCC ACCAATCAGA TAGCAGCTCA GCGGACGCCC TCATCCACCA ATCAGAAAGC 
AGCAGGCCCG CAGGAAAGGA GCGCGAATGC TTGTGGGCGG GGCTTTGTAG CAGAAGGGGC 
CGGGACTTAT TCCAGCTGGG CGGAGAACTG GATATCGAGC AGATTGAGAG GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156497.1
CDS	208..1344
Translation	MCVAMVIIHTKTLEKKAVDDVTSEASLQPEPLTMSQGTALFSCGIMDSGRWADVGSVCGVQQRPVGSSLESLWDSMREAGATGGISGLLRDLSLSEASPASAAPPSKRQCRSLSCSDELGCRSSWRPQGSRVWTTVEKRRCHSGGSVQRGVFNPSGFPAMQRSSSFSLPAHSSHLEPFTHGFPFQAFSECPQTPQTLYRSHEQICPAEASSPESTPELQRRSGQSGLARSRSQPCVHNHQKIGVKRRRPADSHKQRPSLDLLKMTQKLQDFHSLSCPGFSGDDKTLPSSSPALLNDTCERAQNDSSADAPIHQSESSSEDALIHQSDSSSADALIHQSESSRPAGKERECLWAGLCSRRGRDLFQLGGELDIEQIERN

Target ORF information:

RefSeq Version	XM_005156440.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 53, member B (fam53b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156440.4

ORF Insert Sequence:

ATGTGTGTTG CCATGGTAAT TATTCACACT AAAACACTGG AAAAGAAGGC GGTTGACGAT 
GTAACATCCG AAGCGTCTCT GCAGCCGGAG CCGCTGACCA TGAGCCAGGG GACAGCTCTG 
TTCTCCTGTG GAATCATGGA CTCTGGGCGC TGGGCAGATG TTGGGAGTGT GTGTGGCGTC 
CAGCAGCGGC CTGTGGGCTC CAGTCTGGAG AGCCTGTGGG ACAGCATGCG TGAGGCGGGC 
GCCACTGGCG GCATCAGCGG GTTACTGCGG GATCTGAGTC TGAGCGAAGC GTCTCCTGCC 
AGTGCTGCGC CGCCCAGCAA ACGCCAGTGC CGCTCGCTGT CCTGCTCGGA TGAGCTGGGC 
TGCCGCTCAT CCTGGCGTCC GCAGGGTTCG CGCGTCTGGA CAACGGTGGA AAAGCGGCGC 
TGCCACAGTG GAGGGAGCGT CCAGAGAGGA GTCTTCAATC CATCAGGCTT TCCTGCAATG 
CAGCGCAGCT CCAGCTTCAG TCTGCCGGCG CACAGCAGTC ATCTGGAGCC CTTCACTCAC 
GGTTTCCCAT TCCAGGCATT CTCCGAGTGT CCGCAGACGC CGCAGACGCT GTACCGTTCA 
CATGAGCAGA TCTGCCCGGC GGAGGCCAGC TCCCCTGAAT CCACCCCAGA GCTCCAGCGC 
CGCAGCGGGC AGAGCGGACT CGCACGCAGC CGATCACAGC CCTGCGTACA CAACCATCAG 
AAGATCGGCG TCAAGCGCAG GAGACCGGCC GACTCGCACA AACAGAGGCC CTCGCTGGAC 
CTGCTCAAGA TGACACAGAA GCTGCAGGAT TTTCACAGTC TCAGCTGTCC AGGATTCAGC 
GGCGACGACA AAACTCTGCC GTCCAGCAGC CCGGCACTCC TCAACGACAC ATGTGAGCGT 
GCTCAGAATG ACAGCTCAGC GGATGCCCCC ATCCACCAAT CAGAGAGCAG CTCAGAGGAC 
GCCCTCATCC ACCAATCAGA TAGCAGCTCA GCGGACGCCC TCATCCACCA ATCAGAAAGC 
AGCAGGCCCG CAGGAAAGGA GCGCGAATGC TTGTGGGCGG GGCTTTGTAG CAGAAGGGGC 
CGGGACTTAT TCCAGCTGGG CGGAGAACTG GATATCGAGC AGATTGAGAG GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Simplet/Fam53b is required for Wnt signal transduction by regulating ?-catenin nuclear localization.
Development (Cambridge, England)141(18)3529-39(2014 Sep)
Kizil C,K?chler B,Yan JJ,?zhan G,Moro E,Argenton F,Brand M,Weidinger G,Antos CL

Identification of possible downstream genes required for the extension of peripheral axons in primary sensory neurons.
Biochemical and biophysical research communications445(2)357-62(2014 Mar)
Aoki M,Segawa H,Naito M,Okamoto H

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Genome research21(8)1328-38(2011 Aug)
Aanes H,Winata CL,Lin CH,Chen JP,Srinivasan KG,Lee SG,Lim AY,Hajan HS,Collas P,Bourque G,Gong Z,Korzh V,Alestr?m P,Mathavan S

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fam53b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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