irf10 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	irf10
Entrez Gene ID	405815
Full Name	interferon regulatory factor 10
Synonyms	zgc:76891
General protein information	Preferred Names interferon regulatory factor 10 Names interferon regulatory factor 10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161894.2	XP_005161951.1	interferon regulatory factor 10 isoform X1
NM_212879.1	NP_998044.1	interferon regulatory factor 10
XM_005161894.3	XP_005161951.1	interferon regulatory factor 10 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	irf10	NP_998044.1
Bos taurus (cattle)	LOC618409	XP_002692330.2
Canis lupus familiaris (dog)	LOC609817	XP_005635402.1
Gallus gallus (chicken)	IRF10	NP_989889.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost.
Ai K, Luo K, Li Y, Hu W, Gao W, Fang L, Tian G, Ruan G, Xu Q
Fish & shellfish immunology6059-64(2017 Jan)

Expression profile of carp IFN correlate with the up-regulation of interferon regulatory factor-1 (IRF-1) in vivo and in vitro: the pivotal molecules in antiviral defense.
Shan S, Qi C, Zhu Y, Li H, An L, Yang G
Fish & shellfish immunology5294-102(2016 May)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Feng H, Zhang YB, Zhang QM, Li Z, Zhang QY, Gui JF
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IRF10 inhibits the expression of interferon (IFN)1 and IFN3 to avoid an excessive immune response, a unique regulation mechanism of the IFN responses in lower vertebrates.
Title: IFN regulatory factor 10 is a negative regulator of the IFN responses in fish.

The following irf10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irf10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31354	NM_212879.1 Latest version!	Danio rerio interferon regulatory factor 10 (irf10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa27302	XM_005161894.3 Latest version!	Danio rerio interferon regulatory factor 10 (irf10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa27302	XM_005161894.2	Danio rerio interferon regulatory factor 10 (irf10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

irf10 ( NM_212879.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998044.1 Danio rerio interferon regulatory factor 10 (irf10), mRNA.

Price & Availability↑

CloneID	ODa31354
Clone ID Related Accession (Same CDS sequence)	NM_212879.1
Accession Version	NM_212879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	interferon regulatory factor 10
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066724.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066724.1, BC153458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGACA GGTCCAGACA CATGCGCCTC AGAGAGTGGC TGATAGCGCA GATAGACAGC 
GCAGAATACC CGGGGCTGAG CTGGGAGAAC GCGGAGAAAA GCATGTTCAG GATCCCGTGG 
AAACACGCCG CCAAACAGGA CTACAGACAG AACCAGGACG CGGCTCTGTT TAAGGCTTGG 
GCCATGTACA AGGGTAAATT TCAGGAGGGC AGGGATAAAG CAGACCCGTC CACATGGAAA 
ACTCGCCTAC GCTGCGCCCT GAACAAGAGC ACGGACTTCC AGGAGGTTTC AGAGCGCAGT 
CAGCTGGACA TCTCTGAGCC CTACAAGGTG TACCGGATCC TGGAGGACAG CGCACGGGGG 
ACAGACTCTG CTGTAAATCC AGTAATATCG CAGGACAGCG ACTGCAGTAA AGCGCTCAGA 
GAGACACACC TGTCCATGCA GGAGGACGAG TCCGACAGCG GTGCTGGATG GACAGTGAAT 
GGGACGGCAT CTGTGTGTTC TGGAACAGGA CCCAAACCCT GCGCTAATCT TCAACCTGCT 
GCTTTATATC CGCCTCAGGT CCACTTATCA GTGGACTGTC GTCTGGAGCT GCGTGTGTTC 
TACTATGGGC GTGTGGTGGA GAGTGTGCTC AGCTGTTCTC CAGACGGGTG CTTCCTCCTA 
CAGGGCTGTG CTCCGGTGGG CAGCGAGCGC ATCTACGGCC CCTGCGCAGC CACGCAGCTC 
TTCTTCCCGC CACCGAACGC CGCTATGCTG CCCACCGGGA TCTGCGAGGC CATGACGCGC 
CTCCTGCCGC ACCTGGAGAA AGGTGTGCTG CTGTGGGTCG CCCCCGATGG CCTGTTCATC 
AAGCGCTTCT GCCAGGGCCG CGTGTACTGG GACGGCCCGC TGGCGGAGCA CAGACACAAA 
CCCAACAAAC TGGAGAGAGA GAGAACCTGC AAACTGCTGG ACATGAAGAT CTTCAGCCAG 
GAGTTACTGA ACTACCGTCA GGGCATCGGG CCCGAGCCGC AGTACATTGT GGAGCTGTGC 
TTCGGAGAGG AGTTTCCAGA CCCCACACAG CCCAAAAACA AGAAGCTCAT TAGGGCGCAA 
GTGACTCCTA TGTTTGCTGT GGACGCTCTG CGCAAGCTTA AAGCTGATAA TAATGTGGAG 
ATGAAGCCCC CACACCCCCT CGCACAGGAA AACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998044.1
CDS	325..1503
Translation	MEDRSRHMRLREWLIAQIDSAEYPGLSWENAEKSMFRIPWKHAAKQDYRQNQDAALFKAWAMYKGKFQEGRDKADPSTWKTRLRCALNKSTDFQEVSERSQLDISEPYKVYRILEDSARGTDSAVNPVISQDSDCSKALRETHLSMQEDESDSGAGWTVNGTASVCSGTGPKPCANLQPAALYPPQVHLSVDCRLELRVFYYGRVVESVLSCSPDGCFLLQGCAPVGSERIYGPCAATQLFFPPPNAAMLPTGICEAMTRLLPHLEKGVLLWVAPDGLFIKRFCQGRVYWDGPLAEHRHKPNKLERERTCKLLDMKIFSQELLNYRQGIGPEPQYIVELCFGEEFPDPTQPKNKKLIRAQVTPMFAVDALRKLKADNNVEMKPPHPLAQENQ

Target ORF information:

RefSeq Version	NM_212879.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interferon regulatory factor 10 (irf10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212879.1

ORF Insert Sequence:

ATGGAGGACA GGTCCAGACA CATGCGCCTC AGAGAGTGGC TGATAGCGCA GATAGACAGC 
GCAGAATACC CGGGGCTGAG CTGGGAGAAC GCGGAGAAAA GCATGTTCAG GATCCCGTGG 
AAACACGCCG CCAAACAGGA CTACAGACAG AACCAGGACG CGGCTCTGTT TAAGGCTTGG 
GCCATGTACA AGGGTAAATT TCAGGAGGGC AGGGATAAAG CAGACCCGTC CACATGGAAA 
ACTCGCCTAC GCTGCGCCCT GAACAAGAGC ACGGACTTCC AGGAGGTTTC AGAGCGCAGT 
CAGCTGGACA TCTCTGAGCC CTACAAGGTG TACCGGATCC TGGAGGACAG CGCACGGGGG 
ACAGACTCTG CTGTAAATCC AGTAATATCG CAGGACAGCG ACTGCAGTAA AGCGCTCAGA 
GAGACACACC TGTCCATGCA GGAGGACGAG TCCGACAGCG GTGCTGGATG GACAGTGAAT 
GGGACGGCAT CTGTGTGTTC TGGAACAGGA CCCAAACCCT GCGCTAATCT TCAACCTGCT 
GCTTTATATC CGCCTCAGGT CCACTTATCA GTGGACTGTC GTCTGGAGCT GCGTGTGTTC 
TACTATGGGC GTGTGGTGGA GAGTGTGCTC AGCTGTTCTC CAGACGGGTG CTTCCTCCTA 
CAGGGCTGTG CTCCGGTGGG CAGCGAGCGC ATCTACGGCC CCTGCGCAGC CACGCAGCTC 
TTCTTCCCGC CACCGAACGC CGCTATGCTG CCCACCGGGA TCTGCGAGGC CATGACGCGC 
CTCCTGCCGC ACCTGGAGAA AGGTGTGCTG CTGTGGGTCG CCCCCGATGG CCTGTTCATC 
AAGCGCTTCT GCCAGGGCCG CGTGTACTGG GACGGCCCGC TGGCGGAGCA CAGACACAAA 
CCCAACAAAC TGGAGAGAGA GAGAACCTGC AAACTGCTGG ACATGAAGAT CTTCAGCCAG 
GAGTTACTGA ACTACCGTCA GGGCATCGGG CCCGAGCCGC AGTACATTGT GGAGCTGTGC 
TTCGGAGAGG AGTTTCCAGA CCCCACACAG CCCAAAAACA AGAAGCTCAT TAGGGCGCAA 
GTGACTCCTA TGTTTGCTGT GGACGCTCTG CGCAAGCTTA AAGCTGATAA TAATGTGGAG 
ATGAAGCCCC CACACCCCCT CGCACAGGAA AACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irf10 ( XM_005161894.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161951.1 Danio rerio interferon regulatory factor 10 (irf10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27302
Clone ID Related Accession (Same CDS sequence)	XM_005161894.2 , XM_005161894.3
Accession Version	XM_005161894.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	interferon regulatory factor 10 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GGTCCAGACA CATGCGCCTC AGAGAGTGGC TGATAGCGCA GATAGACAGC 
GCAGAATACC CGGGGCTGAG CTGGGAGAAC GCGGAGAAAA GCATGTTCAG GATCCCGTGG 
AAACACGCCG CCAAACAGGA CTACAGACAG AACCAGGACG CGGCTCTGTT TAAGGCTTGG 
GCCATGTACA AGGGTAAATT TCAGGAGGGC AGGGATAAAG CAGACCCGTC CACATGGAAA 
ACTCGTCTAC GCTGCGCCCT GAACAAGAGC ACGGACTTCC AGGAGGTTTC AGAGCGCAGT 
CAGCTGGACA TCTCTGAGCC CTACAAGGTG TACCGGATCC TGGAGGACAG CGCACGGGGG 
ACAGACTCTG CTGTAAATCC AGTAATATCG CAGGACAGTG ACTGCAGTAA AGCACTCAGA 
GAGACACACC TGTCCATGCA GGAGGACGAG TCTGACAGCG GTGCTGGATG GACAGTGAAT 
GGGACGGCAT CTGTGTGTTC TGGAACAGGA GCCAAACCCT GTGTTAATCT CCAACCTGCT 
GCTTTATATC CGCCTCAGGT CCACTTATCA GTGGACTGTC GTCTGGAGCT GCGTGTGTTC 
TACTATGGGC GTGTGGTGGA GAGTGTGCTC AGCTGTTCTC CAGACGGGTG CTTCCTCCTA 
CAGGGCTGTG CTCCGGTGGG CAGCGAGCGC ATCTACGGCC CCTGCGCAGC CACACAGCTC 
TTCTTCCCGC CACCGAACGC CGCTATGCTG CCCACCGGGA TCTGCGAGGC CATGACGCGC 
CTCCTGCCGC ACCTGGAGAA AGGTGTGCTG CTGTGGGTCG CCCCCGATGG CCTGTTCATC 
AAGCGCTTCT GCCAGGGCCG CGTGTACTGG GACGGCCCGC TGGCGGAGCA CAGACACAAA 
CCCAACAAAC TGGAGAGAGA GAGAACCTGC AAACTGCTGG ACATGAAGAT CTTCAGCCAG 
GAGTTACTGA ACTACCGTCA GGGCATCGGG CCCGAGCCGC AGTACATTGT GGAGCTGTGC 
TTCGGAGAGG AGTTTCCAGA CCCCACACAG CCCAAAAACA AGAAGCTCAT CAGGGCGCAA 
GTGACTCCTA TGTTTGCTGT GGACGCTCTG CGCAAGCTTA AAGCTGATAA TAATGTGGAG 
ATGAAGCCCC CACACCCCCT CGCACAGGAA AACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161951.1
CDS	346..1524
Translation	MEDRSRHMRLREWLIAQIDSAEYPGLSWENAEKSMFRIPWKHAAKQDYRQNQDAALFKAWAMYKGKFQEGRDKADPSTWKTRLRCALNKSTDFQEVSERSQLDISEPYKVYRILEDSARGTDSAVNPVISQDSDCSKALRETHLSMQEDESDSGAGWTVNGTASVCSGTGAKPCVNLQPAALYPPQVHLSVDCRLELRVFYYGRVVESVLSCSPDGCFLLQGCAPVGSERIYGPCAATQLFFPPPNAAMLPTGICEAMTRLLPHLEKGVLLWVAPDGLFIKRFCQGRVYWDGPLAEHRHKPNKLERERTCKLLDMKIFSQELLNYRQGIGPEPQYIVELCFGEEFPDPTQPKNKKLIRAQVTPMFAVDALRKLKADNNVEMKPPHPLAQENQ

Target ORF information:

RefSeq Version	XM_005161894.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio interferon regulatory factor 10 (irf10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161894.2

ORF Insert Sequence:

ATGGAGGACA GGTCCAGACA CATGCGCCTC AGAGAGTGGC TGATAGCGCA GATAGACAGC 
GCAGAATACC CGGGGCTGAG CTGGGAGAAC GCGGAGAAAA GCATGTTCAG GATCCCGTGG 
AAACACGCCG CCAAACAGGA CTACAGACAG AACCAGGACG CGGCTCTGTT TAAGGCTTGG 
GCCATGTACA AGGGTAAATT TCAGGAGGGC AGGGATAAAG CAGACCCGTC CACATGGAAA 
ACTCGTCTAC GCTGCGCCCT GAACAAGAGC ACGGACTTCC AGGAGGTTTC AGAGCGCAGT 
CAGCTGGACA TCTCTGAGCC CTACAAGGTG TACCGGATCC TGGAGGACAG CGCACGGGGG 
ACAGACTCTG CTGTAAATCC AGTAATATCG CAGGACAGTG ACTGCAGTAA AGCACTCAGA 
GAGACACACC TGTCCATGCA GGAGGACGAG TCTGACAGCG GTGCTGGATG GACAGTGAAT 
GGGACGGCAT CTGTGTGTTC TGGAACAGGA GCCAAACCCT GTGTTAATCT CCAACCTGCT 
GCTTTATATC CGCCTCAGGT CCACTTATCA GTGGACTGTC GTCTGGAGCT GCGTGTGTTC 
TACTATGGGC GTGTGGTGGA GAGTGTGCTC AGCTGTTCTC CAGACGGGTG CTTCCTCCTA 
CAGGGCTGTG CTCCGGTGGG CAGCGAGCGC ATCTACGGCC CCTGCGCAGC CACACAGCTC 
TTCTTCCCGC CACCGAACGC CGCTATGCTG CCCACCGGGA TCTGCGAGGC CATGACGCGC 
CTCCTGCCGC ACCTGGAGAA AGGTGTGCTG CTGTGGGTCG CCCCCGATGG CCTGTTCATC 
AAGCGCTTCT GCCAGGGCCG CGTGTACTGG GACGGCCCGC TGGCGGAGCA CAGACACAAA 
CCCAACAAAC TGGAGAGAGA GAGAACCTGC AAACTGCTGG ACATGAAGAT CTTCAGCCAG 
GAGTTACTGA ACTACCGTCA GGGCATCGGG CCCGAGCCGC AGTACATTGT GGAGCTGTGC 
TTCGGAGAGG AGTTTCCAGA CCCCACACAG CCCAAAAACA AGAAGCTCAT CAGGGCGCAA 
GTGACTCCTA TGTTTGCTGT GGACGCTCTG CGCAAGCTTA AAGCTGATAA TAATGTGGAG 
ATGAAGCCCC CACACCCCCT CGCACAGGAA AACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irf10 ( XM_005161894.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161951.1 Danio rerio interferon regulatory factor 10 (irf10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27302
Clone ID Related Accession (Same CDS sequence)	XM_005161894.2 , XM_005161894.3
Accession Version	XM_005161894.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	interferon regulatory factor 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GGTCCAGACA CATGCGCCTC AGAGAGTGGC TGATAGCGCA GATAGACAGC 
GCAGAATACC CGGGGCTGAG CTGGGAGAAC GCGGAGAAAA GCATGTTCAG GATCCCGTGG 
AAACACGCCG CCAAACAGGA CTACAGACAG AACCAGGACG CGGCTCTGTT TAAGGCTTGG 
GCCATGTACA AGGGTAAATT TCAGGAGGGC AGGGATAAAG CAGACCCGTC CACATGGAAA 
ACTCGTCTAC GCTGCGCCCT GAACAAGAGC ACGGACTTCC AGGAGGTTTC AGAGCGCAGT 
CAGCTGGACA TCTCTGAGCC CTACAAGGTG TACCGGATCC TGGAGGACAG CGCACGGGGG 
ACAGACTCTG CTGTAAATCC AGTAATATCG CAGGACAGTG ACTGCAGTAA AGCACTCAGA 
GAGACACACC TGTCCATGCA GGAGGACGAG TCTGACAGCG GTGCTGGATG GACAGTGAAT 
GGGACGGCAT CTGTGTGTTC TGGAACAGGA GCCAAACCCT GTGTTAATCT CCAACCTGCT 
GCTTTATATC CGCCTCAGGT CCACTTATCA GTGGACTGTC GTCTGGAGCT GCGTGTGTTC 
TACTATGGGC GTGTGGTGGA GAGTGTGCTC AGCTGTTCTC CAGACGGGTG CTTCCTCCTA 
CAGGGCTGTG CTCCGGTGGG CAGCGAGCGC ATCTACGGCC CCTGCGCAGC CACACAGCTC 
TTCTTCCCGC CACCGAACGC CGCTATGCTG CCCACCGGGA TCTGCGAGGC CATGACGCGC 
CTCCTGCCGC ACCTGGAGAA AGGTGTGCTG CTGTGGGTCG CCCCCGATGG CCTGTTCATC 
AAGCGCTTCT GCCAGGGCCG CGTGTACTGG GACGGCCCGC TGGCGGAGCA CAGACACAAA 
CCCAACAAAC TGGAGAGAGA GAGAACCTGC AAACTGCTGG ACATGAAGAT CTTCAGCCAG 
GAGTTACTGA ACTACCGTCA GGGCATCGGG CCCGAGCCGC AGTACATTGT GGAGCTGTGC 
TTCGGAGAGG AGTTTCCAGA CCCCACACAG CCCAAAAACA AGAAGCTCAT CAGGGCGCAA 
GTGACTCCTA TGTTTGCTGT GGACGCTCTG CGCAAGCTTA AAGCTGATAA TAATGTGGAG 
ATGAAGCCCC CACACCCCCT CGCACAGGAA AACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161951.1
CDS	354..1532
Translation	MEDRSRHMRLREWLIAQIDSAEYPGLSWENAEKSMFRIPWKHAAKQDYRQNQDAALFKAWAMYKGKFQEGRDKADPSTWKTRLRCALNKSTDFQEVSERSQLDISEPYKVYRILEDSARGTDSAVNPVISQDSDCSKALRETHLSMQEDESDSGAGWTVNGTASVCSGTGAKPCVNLQPAALYPPQVHLSVDCRLELRVFYYGRVVESVLSCSPDGCFLLQGCAPVGSERIYGPCAATQLFFPPPNAAMLPTGICEAMTRLLPHLEKGVLLWVAPDGLFIKRFCQGRVYWDGPLAEHRHKPNKLERERTCKLLDMKIFSQELLNYRQGIGPEPQYIVELCFGEEFPDPTQPKNKKLIRAQVTPMFAVDALRKLKADNNVEMKPPHPLAQENQ

Target ORF information:

RefSeq Version	XM_005161894.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interferon regulatory factor 10 (irf10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161894.3

ORF Insert Sequence:

ATGGAGGACA GGTCCAGACA CATGCGCCTC AGAGAGTGGC TGATAGCGCA GATAGACAGC 
GCAGAATACC CGGGGCTGAG CTGGGAGAAC GCGGAGAAAA GCATGTTCAG GATCCCGTGG 
AAACACGCCG CCAAACAGGA CTACAGACAG AACCAGGACG CGGCTCTGTT TAAGGCTTGG 
GCCATGTACA AGGGTAAATT TCAGGAGGGC AGGGATAAAG CAGACCCGTC CACATGGAAA 
ACTCGTCTAC GCTGCGCCCT GAACAAGAGC ACGGACTTCC AGGAGGTTTC AGAGCGCAGT 
CAGCTGGACA TCTCTGAGCC CTACAAGGTG TACCGGATCC TGGAGGACAG CGCACGGGGG 
ACAGACTCTG CTGTAAATCC AGTAATATCG CAGGACAGTG ACTGCAGTAA AGCACTCAGA 
GAGACACACC TGTCCATGCA GGAGGACGAG TCTGACAGCG GTGCTGGATG GACAGTGAAT 
GGGACGGCAT CTGTGTGTTC TGGAACAGGA GCCAAACCCT GTGTTAATCT CCAACCTGCT 
GCTTTATATC CGCCTCAGGT CCACTTATCA GTGGACTGTC GTCTGGAGCT GCGTGTGTTC 
TACTATGGGC GTGTGGTGGA GAGTGTGCTC AGCTGTTCTC CAGACGGGTG CTTCCTCCTA 
CAGGGCTGTG CTCCGGTGGG CAGCGAGCGC ATCTACGGCC CCTGCGCAGC CACACAGCTC 
TTCTTCCCGC CACCGAACGC CGCTATGCTG CCCACCGGGA TCTGCGAGGC CATGACGCGC 
CTCCTGCCGC ACCTGGAGAA AGGTGTGCTG CTGTGGGTCG CCCCCGATGG CCTGTTCATC 
AAGCGCTTCT GCCAGGGCCG CGTGTACTGG GACGGCCCGC TGGCGGAGCA CAGACACAAA 
CCCAACAAAC TGGAGAGAGA GAGAACCTGC AAACTGCTGG ACATGAAGAT CTTCAGCCAG 
GAGTTACTGA ACTACCGTCA GGGCATCGGG CCCGAGCCGC AGTACATTGT GGAGCTGTGC 
TTCGGAGAGG AGTTTCCAGA CCCCACACAG CCCAAAAACA AGAAGCTCAT CAGGGCGCAA 
GTGACTCCTA TGTTTGCTGT GGACGCTCTG CGCAAGCTTA AAGCTGATAA TAATGTGGAG 
ATGAAGCCCC CACACCCCCT CGCACAGGAA AACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost.
Fish & shellfish immunology6059-64(2017 Jan)
Ai K,Luo K,Li Y,Hu W,Gao W,Fang L,Tian G,Ruan G,Xu Q

Expression profile of carp IFN correlate with the up-regulation of interferon regulatory factor-1 (IRF-1) in vivo and in vitro: the pivotal molecules in antiviral defense.
Fish & shellfish immunology5294-102(2016 May)
Shan S,Qi C,Zhu Y,Li H,An L,Yang G

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)
Feng H,Zhang YB,Zhang QM,Li Z,Zhang QY,Gui JF

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irf10 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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