tlr21 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tlr21
Entrez Gene ID	402884
Full Name	toll-like receptor 21
Synonyms	TLR21.1
General protein information	Preferred Names toll-like receptor 21 Names toll-like receptor 21 TIR21.1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199335.1	NP_001186264.1	toll-like receptor 21 precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497009	XP_002936443.2
Danio rerio (zebrafish)	tlr21	NP_001186264.1
Gallus gallus (chicken)	TLR21	NP_001025729.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio).
Yoon S, Mitra S, Wyse C, Alnabulsi A, Zou J, Weerdenburg EM, van der Sar AM, Wang D, Secombes CJ, Bird S
PloS one10(6)e0126378(2015)

Identification and functional characterization of nonmammalian Toll-like receptor 20.
Pietretti D, Scheer M, Fink IR, Taverne N, Savelkoul HF, Spaink HP, Forlenza M, Wiegertjes GF
Immunogenetics66(2)123-41(2014 Feb)

Toll-like receptor 9 and 21 have different ligand recognition profiles and cooperatively mediate activity of CpG-oligodeoxynucleotides in zebrafish.
Yeh DW, Liu YL, Lo YC, Yuh CH, Yu GY, Lo JF, Luo Y, Xiang R, Chuang TH
Proceedings of the National Academy of Sciences of the United States of America110(51)20711-6(2013 Dec)

Molecular characterization and expression analysis of Toll-like receptor 21 cDNA from Paralichthys olivaceus.
Gao H, Wu L, Sun JS, Geng XY, Pan BP
Fish & shellfish immunology35(4)1138-45(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that zebTLR9 and zebTLR21 cooperatively mediate the antimicrobial activities of CpG-ODN. Overall, this study provides a molecular basis for the activities of CpG-ODN in fish.
Title: Toll-like receptor 9 and 21 have different ligand recognition profiles and cooperatively mediate activity of CpG-oligodeoxynucleotides in zebrafish.

The following tlr21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tlr21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00694	NM_001199335.1 Latest version!	Danio rerio toll-like receptor 21 (tlr21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tlr21 ( NM_001199335.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001186264.1 Danio rerio toll-like receptor 21 (tlr21), mRNA.

Price & Availability↑

CloneID	ODa00694
Clone ID Related Accession (Same CDS sequence)	NM_001199335.1
Accession Version	NM_001199335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	toll-like receptor 21 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX571826.20. On Dec 5, 2010 this sequence version replaced XM_001923192.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCACACT CTGCATGCCA CAAACTGATA CTAAAGGCCA CATTCATCTG TCTCATAAAA 
CTTGCCTGCA GCTACAGTTT CAGAAGTTGC ATAGAGATCC CAGATTCTAA TCATACAATC 
TTTACATGTG TTAAAAGTTA TGAACGAGAC ATAACTGCGA TTGTGAGTGA TGTATTTCCC 
ACTGCATTAA ATCTTACAAT CTCTCACAGC AACATTGAAT ATGTGCAAAG TCAAAGCTTC 
GATCATCTGC CCAATCTGAC TGCTCTGATA CTGGACGCTA ACCATATTAC TAACATCGAC 
AAGGATGCTT TTAATAATCT ACACCATCTT CAGACTTTAA ATTTGTCCTG CAACAACATA 
TCATTTCTCC ACAGGGATGT CTTCAGTGAC CTCCATAACC TCACACAGCT GCTCCTGGGA 
AGCAATAAAC TGACTAATCC TGATAAGTAT CTGTTTTCTA GTCTAACAAA CCTCAAGATT 
CTTGACTTAC GCAGAAACCA CTTGTATAAC TTTTCGTCTG TGGTTGAAAG CATAACAAAC 
CTGACAAGCT TGATAAAACT GGATCTTTCT TTTAACAAGC TGACCACCCT GCAACACTCC 
ACCCATCTGC CAGAGTCACT TGCCTTGCTC TACCTCGGTG ATAACAAACT AAAGACACTG 
GGATGTGACA AGTCTTTTCT TATGAATGTC AAAAAGCTGG ATCTCTCAGA CAACAAAAAC 
CTGGCATCCA AATCTTTTCT TGGCCTGAAA CTGGAGAATA TAACGTACCT GCGGTTGCGC 
TTTACCAATA TCTCCATTTT CAAACTTCTT ACTTATCCCA ATGTGCATCC GTGGCACATT 
GACTTTTCTG GCCTGAAGCT GAAGAACTCC CATGCACTGA GTTCCCTGTG TGTCCTTATG 
CGAAATCACA GCCATAAGCA TTTACAAATA CCTAAAATGT TGCTCCAGAG CAATTCTATA 
GGAGCTCTAA AAAATAATAC ATTTCGTTAT TGCCCCAGTA TCACACGGGA AATGGACCTC 
TCGCGGAATG AGCTGAAAAG CGCTGGCTGC TTGCAATTTC TCCACAATCA GACTCAACTG 
AAAAGTCTCA AGATGGAGCA TAATCATCTG ACCAAGCTAA CGTCCTGCAA CAAAGCAGAA 
ATACATAATA GCCTGACAAA CCTGAGCTAT CGATACAACC GTATTCTACA GATCAGTGGT 
TATGCCTTCA GCAATACACC TAAATTAGAA ATACTGGAGC TGAACATCAA CATAATTGCT 
TATATGGACC ATAAAGCCTT AAGTGGGCTC AAAGATCTCA AAACTCTTCG TCTCGACAAC 
AACCTTTTGA GTGATATCTA CAATGATAGC TTTGAAGATC TCACAAACCT GAAGACTCTG 
AACCTGCGCA ACAATGAAAT AGCTGTCATT TTCAACTACA CCTTTCAGAA TCTGTCTAAA 
CTGGATATTT TAGATTTAGG CGGGAACAAG ATCTCACAGC TGAAACCGCA TGCTTTTGAT 
GGGCTCTATA GCTTGACCAA TCTGTATCTA GACAGGAATC ATCTTAAAAT GATTGATGTC 
GTACTCTTTG GGAAACTCTC TGGCACCCTG CAGGTGCTGG ATTTGCAGGG TAACATGATT 
ATGTATTTTA CAGAACATAC ACACTCGCCG TTTAAAAATA TGTCAAGACT TTACGACCTG 
AAACTTAATG GACAGCAACC TAACGGCATT AACTTGTTGC CTCGTGCATT TTTCCGTGGT 
CTAACTTCTT TGAAAAGTCT GTATCTCACC AACAATCATA TTATTGGATT TGGTGATGAA 
ACGTTCGACG ACCTGAAAAA CTTGGATTAT CTAACACTGG ACAACTCGTG TGCTGGGGTT 
GCCCAGCTCC AATCTGGAAT TTTCAAAAGC CTTCAAAAAC TAAGGACGTT ATCTCTTGAG 
AACATGGGTA TTAAATCGTT TTCAAAAGAT GTTTTCGGAA ATCTCACAGG ATTGAAGACG 
CTACATTTGA ATCGCAATCC CATGATGTCT TTGGACATTA GTTTACTGGA CAATCTGACT 
AATCTAATAT ACATCGACAT GCGCAGTTGT CCTCTGAGTT GTGGCTGCCA TAACAGTGAT 
CTACAGAACT GGACCATAAT GAATAAGAGG CTCCAATTTC CACATCTTTT CAATATCACA 
TGCCCAGACC ACCCAGGCTC ATATTTTCAC AACTTTGACA CTAAAGTGTG TTATTTGGAC 
ATAGAGCTTT ACTTTTTTTC CTCAACCTCC ACATTGACAA TTTTATTAAC CTTAATACCA 
CTTCTCTATG TCAAACTTTA CTGGAAATTC AAGTATGGCT ACTATGTGTT TCGATCGTGG 
TTTGGAGAAC AGTGGCGTCG CTTACGGGAC CAGGAGGAAA AATACAATTA CGATGCCTTT 
GTTTCTTACA ATTCAGCAGA TGAAGACTGG GTAATGGAAC AGTTACTGCC GAACCTTGAG 
GGTTCTTCAT TTCGCCTGTG CCTCCACCAC AGAGATTTTG AGCTAGGCCG TGATATTGTC 
GACAACATTG TAGCTGCCGT CTATGGCAGC CGTAAAACAA TCTGTGTGGT CAGTCAGAGC 
TTCCTTCGCA GTGAATGGTG CTCACTAGAG ATTCAATTGG CTAGCTATCG CCTCTTCCAG 
GAAATGCAAG ATGTGCTTTT GCTTGTCTTT CTGGAGCCTA TCCCTGAACG TCAACTGTCC 
GCTTATCATC GCATGAGGAA AGTCATGCTC AAAAAGACCT ATCTTCAGTG GCCTGGGTCA 
AACTGTTCTG ATCCAAACAG TGCAAAAGAA CTGTTCTGGA ATCAGTTAAA GAGAGCTCTA 
AGAAGCAGCA ACTCTGGAAG CCAAGATGAG CAGAAGATGG ATGACAATGA ACTGAGGAGA 
AAAGAGAAAG TGTGTGCGGA CAAGGAGGAA AGAGAATATT TTGTAAATCA GACACCAACA 
GAAGATGAAA TATATTACCT GATGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186264.1
CDS	86..3055
Translation	MAHSACHKLILKATFICLIKLACSYSFRSCIEIPDSNHTIFTCVKSYERDITAIVSDVFPTALNLTISHSNIEYVQSQSFDHLPNLTALILDANHITNIDKDAFNNLHHLQTLNLSCNNISFLHRDVFSDLHNLTQLLLGSNKLTNPDKYLFSSLTNLKILDLRRNHLYNFSSVVESITNLTSLIKLDLSFNKLTTLQHSTHLPESLALLYLGDNKLKTLGCDKSFLMNVKKLDLSDNKNLASKSFLGLKLENITYLRLRFTNISIFKLLTYPNVHPWHIDFSGLKLKNSHALSSLCVLMRNHSHKHLQIPKMLLQSNSIGALKNNTFRYCPSITREMDLSRNELKSAGCLQFLHNQTQLKSLKMEHNHLTKLTSCNKAEIHNSLTNLSYRYNRILQISGYAFSNTPKLEILELNINIIAYMDHKALSGLKDLKTLRLDNNLLSDIYNDSFEDLTNLKTLNLRNNEIAVIFNYTFQNLSKLDILDLGGNKISQLKPHAFDGLYSLTNLYLDRNHLKMIDVVLFGKLSGTLQVLDLQGNMIMYFTEHTHSPFKNMSRLYDLKLNGQQPNGINLLPRAFFRGLTSLKSLYLTNNHIIGFGDETFDDLKNLDYLTLDNSCAGVAQLQSGIFKSLQKLRTLSLENMGIKSFSKDVFGNLTGLKTLHLNRNPMMSLDISLLDNLTNLIYIDMRSCPLSCGCHNSDLQNWTIMNKRLQFPHLFNITCPDHPGSYFHNFDTKVCYLDIELYFFSSTSTLTILLTLIPLLYVKLYWKFKYGYYVFRSWFGEQWRRLRDQEEKYNYDAFVSYNSADEDWVMEQLLPNLEGSSFRLCLHHRDFELGRDIVDNIVAAVYGSRKTICVVSQSFLRSEWCSLEIQLASYRLFQEMQDVLLLVFLEPIPERQLSAYHRMRKVMLKKTYLQWPGSNCSDPNSAKELFWNQLKRALRSSNSGSQDEQKMDDNELRRKEKVCADKEEREYFVNQTPTEDEIYYLMP

Target ORF information:

RefSeq Version	NM_001199335.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio toll-like receptor 21 (tlr21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199335.1

ORF Insert Sequence:

ATGGCACACT CTGCATGCCA CAAACTGATA CTAAAGGCCA CATTCATCTG TCTCATAAAA 
CTTGCCTGCA GCTACAGTTT CAGAAGTTGC ATAGAGATCC CAGATTCTAA TCATACAATC 
TTTACATGTG TTAAAAGTTA TGAACGAGAC ATAACTGCGA TTGTGAGTGA TGTATTTCCC 
ACTGCATTAA ATCTTACAAT CTCTCACAGC AACATTGAAT ATGTGCAAAG TCAAAGCTTC 
GATCATCTGC CCAATCTGAC TGCTCTGATA CTGGACGCTA ACCATATTAC TAACATCGAC 
AAGGATGCTT TTAATAATCT ACACCATCTT CAGACTTTAA ATTTGTCCTG CAACAACATA 
TCATTTCTCC ACAGGGATGT CTTCAGTGAC CTCCATAACC TCACACAGCT GCTCCTGGGA 
AGCAATAAAC TGACTAATCC TGATAAGTAT CTGTTTTCTA GTCTAACAAA CCTCAAGATT 
CTTGACTTAC GCAGAAACCA CTTGTATAAC TTTTCGTCTG TGGTTGAAAG CATAACAAAC 
CTGACAAGCT TGATAAAACT GGATCTTTCT TTTAACAAGC TGACCACCCT GCAACACTCC 
ACCCATCTGC CAGAGTCACT TGCCTTGCTC TACCTCGGTG ATAACAAACT AAAGACACTG 
GGATGTGACA AGTCTTTTCT TATGAATGTC AAAAAGCTGG ATCTCTCAGA CAACAAAAAC 
CTGGCATCCA AATCTTTTCT TGGCCTGAAA CTGGAGAATA TAACGTACCT GCGGTTGCGC 
TTTACCAATA TCTCCATTTT CAAACTTCTT ACTTATCCCA ATGTGCATCC GTGGCACATT 
GACTTTTCTG GCCTGAAGCT GAAGAACTCC CATGCACTGA GTTCCCTGTG TGTCCTTATG 
CGAAATCACA GCCATAAGCA TTTACAAATA CCTAAAATGT TGCTCCAGAG CAATTCTATA 
GGAGCTCTAA AAAATAATAC ATTTCGTTAT TGCCCCAGTA TCACACGGGA AATGGACCTC 
TCGCGGAATG AGCTGAAAAG CGCTGGCTGC TTGCAATTTC TCCACAATCA GACTCAACTG 
AAAAGTCTCA AGATGGAGCA TAATCATCTG ACCAAGCTAA CGTCCTGCAA CAAAGCAGAA 
ATACATAATA GCCTGACAAA CCTGAGCTAT CGATACAACC GTATTCTACA GATCAGTGGT 
TATGCCTTCA GCAATACACC TAAATTAGAA ATACTGGAGC TGAACATCAA CATAATTGCT 
TATATGGACC ATAAAGCCTT AAGTGGGCTC AAAGATCTCA AAACTCTTCG TCTCGACAAC 
AACCTTTTGA GTGATATCTA CAATGATAGC TTTGAAGATC TCACAAACCT GAAGACTCTG 
AACCTGCGCA ACAATGAAAT AGCTGTCATT TTCAACTACA CCTTTCAGAA TCTGTCTAAA 
CTGGATATTT TAGATTTAGG CGGGAACAAG ATCTCACAGC TGAAACCGCA TGCTTTTGAT 
GGGCTCTATA GCTTGACCAA TCTGTATCTA GACAGGAATC ATCTTAAAAT GATTGATGTC 
GTACTCTTTG GGAAACTCTC TGGCACCCTG CAGGTGCTGG ATTTGCAGGG TAACATGATT 
ATGTATTTTA CAGAACATAC ACACTCGCCG TTTAAAAATA TGTCAAGACT TTACGACCTG 
AAACTTAATG GACAGCAACC TAACGGCATT AACTTGTTGC CTCGTGCATT TTTCCGTGGT 
CTAACTTCTT TGAAAAGTCT GTATCTCACC AACAATCATA TTATTGGATT TGGTGATGAA 
ACGTTCGACG ACCTGAAAAA CTTGGATTAT CTAACACTGG ACAACTCGTG TGCTGGGGTT 
GCCCAGCTCC AATCTGGAAT TTTCAAAAGC CTTCAAAAAC TAAGGACGTT ATCTCTTGAG 
AACATGGGTA TTAAATCGTT TTCAAAAGAT GTTTTCGGAA ATCTCACAGG ATTGAAGACG 
CTACATTTGA ATCGCAATCC CATGATGTCT TTGGACATTA GTTTACTGGA CAATCTGACT 
AATCTAATAT ACATCGACAT GCGCAGTTGT CCTCTGAGTT GTGGCTGCCA TAACAGTGAT 
CTACAGAACT GGACCATAAT GAATAAGAGG CTCCAATTTC CACATCTTTT CAATATCACA 
TGCCCAGACC ACCCAGGCTC ATATTTTCAC AACTTTGACA CTAAAGTGTG TTATTTGGAC 
ATAGAGCTTT ACTTTTTTTC CTCAACCTCC ACATTGACAA TTTTATTAAC CTTAATACCA 
CTTCTCTATG TCAAACTTTA CTGGAAATTC AAGTATGGCT ACTATGTGTT TCGATCGTGG 
TTTGGAGAAC AGTGGCGTCG CTTACGGGAC CAGGAGGAAA AATACAATTA CGATGCCTTT 
GTTTCTTACA ATTCAGCAGA TGAAGACTGG GTAATGGAAC AGTTACTGCC GAACCTTGAG 
GGTTCTTCAT TTCGCCTGTG CCTCCACCAC AGAGATTTTG AGCTAGGCCG TGATATTGTC 
GACAACATTG TAGCTGCCGT CTATGGCAGC CGTAAAACAA TCTGTGTGGT CAGTCAGAGC 
TTCCTTCGCA GTGAATGGTG CTCACTAGAG ATTCAATTGG CTAGCTATCG CCTCTTCCAG 
GAAATGCAAG ATGTGCTTTT GCTTGTCTTT CTGGAGCCTA TCCCTGAACG TCAACTGTCC 
GCTTATCATC GCATGAGGAA AGTCATGCTC AAAAAGACCT ATCTTCAGTG GCCTGGGTCA 
AACTGTTCTG ATCCAAACAG TGCAAAAGAA CTGTTCTGGA ATCAGTTAAA GAGAGCTCTA 
AGAAGCAGCA ACTCTGGAAG CCAAGATGAG CAGAAGATGG ATGACAATGA ACTGAGGAGA 
AAAGAGAAAG TGTGTGCGGA CAAGGAGGAA AGAGAATATT TTGTAAATCA GACACCAACA 
GAAGATGAAA TATATTACCT GATGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio).
PloS one10(6)e0126378(2015)
Yoon S,Mitra S,Wyse C,Alnabulsi A,Zou J,Weerdenburg EM,van der Sar AM,Wang D,Secombes CJ,Bird S

Identification and functional characterization of nonmammalian Toll-like receptor 20.
Immunogenetics66(2)123-41(2014 Feb)
Pietretti D,Scheer M,Fink IR,Taverne N,Savelkoul HF,Spaink HP,Forlenza M,Wiegertjes GF

Toll-like receptor 9 and 21 have different ligand recognition profiles and cooperatively mediate activity of CpG-oligodeoxynucleotides in zebrafish.
Proceedings of the National Academy of Sciences of the United States of America110(51)20711-6(2013 Dec)
Yeh DW,Liu YL,Lo YC,Yuh CH,Yu GY,Lo JF,Luo Y,Xiang R,Chuang TH

Molecular characterization and expression analysis of Toll-like receptor 21 cDNA from Paralichthys olivaceus.
Fish & shellfish immunology35(4)1138-45(2013 Oct)
Gao H,Wu L,Sun JS,Geng XY,Pan BP

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

tlr21 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報