prrc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prrc2a
Entrez Gene ID	394248
Full Name	proline-rich coiled-coil 2A
Synonyms	bat2,wu:fb96a05,wu:fb96a11
General protein information	Preferred Names proline-rich coiled-coil 2A Names protein PRRC2A HLA-B-associated transcript 2 fb96a05
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170017.4	XP_005170074.1	protein PRRC2A isoform X1
XM_005170017.3	XP_005170074.1	protein PRRC2A isoform X1
XM_005170015.4	XP_005170072.1	protein PRRC2A isoform X2
XM_009294206.2	XP_009292481.1	protein PRRC2A isoform X1
XM_005170015.3	XP_005170072.1	protein PRRC2A isoform X2
XM_009294206.3	XP_009292481.1	protein PRRC2A isoform X1
NM_201347.3	NP_958504.2	protein PRRC2A isoform 1
NM_001365423.1	NP_001352352.1	protein PRRC2A isoform 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Presence of two tumor necrosis factor (tnf)-? homologs on different chromosomes of zebrafish (Danio rerio) and medaka (Oryzias latipes).
Kinoshita S, Biswas G, Kono T, Hikima J, Sakai M
Marine genomics131-9(2014 Feb)

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish.
Sambrook JG, Figueroa F, Beck S
BMC genomics6152(2005 Nov)

Conservation of Mhc class III region synteny between zebrafish and human as determined by radiation hybrid mapping.
S?ltmann H, Sato A, Murray BW, Takezaki N, Geisler R, Rauch GJ, Klein J
Journal of immunology (Baltimore, Md. : 1950)165(12)6984-93(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prrc2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prrc2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24123	NM_001365423.1 Latest version!	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa36388	NM_201347.3 Latest version!	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa24122	XM_005170015.4 Latest version!	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa24123	XM_009294206.3 Latest version!	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa24123	XM_005170017.4 Latest version!	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa24123	XM_005170017.3	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa24122	XM_005170015.3	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa24123	XM_009294206.2	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prrc2a ( NM_001365423.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352352.1 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa24123
Clone ID Related Accession (Same CDS sequence)	XM_005170017.4 , XM_005170017.3 , XM_009294206.2 , XM_009294206.3 , NM_001365423.1
Accession Version	NM_001365423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	protein PRRC2A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP085398.5 and FP017214.1. On Aug 24, 2018 this sequence version replaced XM_005170017.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01008916.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352352.1
CDS	259..5937
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFVSMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	NM_001365423.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365423.1

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2a ( NM_201347.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_958504.2 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant 1, mRNA.

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CloneID	ODa36388
Clone ID Related Accession (Same CDS sequence)	NM_201347.3
Accession Version	NM_201347.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	protein PRRC2A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP085398.5 and FP017214.1. On Mar 7, 2013 this sequence version replaced NM_201347.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CATGTATCCA CCATACCTGC CCTTTCCACC CCCTTATGGT 
CCACAGGGAC CTTACAGGTA CCCACCGCCA AATGAGGCTC CTAGGTTTTC TCGGAACCAG 
GGTGGAATAG GGCCAGATGG GCGACCCCCT GGAGGCCCCC GGGGTGAAGT GGTAAAACGC 
CCATCCATCC TCAAACAGGA TGACCTAAAA GAGCTGGATG AGCTGGACCA TGATGGAGAT 
GAGGGCTGGG CTGGAGCTCA AGAGGAAATT GACTACTCCG CGAAGTTGAA ATTCAGTGAC 
GATGAAGGAG AGGATGATGG GGAGGAGGAA CGGGGTGAAA ATAAAAATGG AACCCGGGAG 
TCAAGGGAAC AGCAGAGATC CCAAGATGGA CCTGTACCTG TTTCACGTTC CAGGGCATCC 
GACAGTGGAG GGGATTCGCG ACGCACCCCT CCTTCCAGTA CTGAAGATGG CTCTGTGTCC 
CCCTCCAGCA AGCCAGGCTG GGCCGAGGAG GGAGGTAGCA GCTGGAACAG TCAAGCAAAT 
GCCGGATCTT ATCAGGGGCC TAGGCCTGGA TTAGGTGGCA CTAGGGAGCA GCCCTCTCCC 
CCACCAGGTC CACTTCTTGG ACAAGGGCCC CACTCCTACT ACCGCCAGGA CCGGCCTTCA 
AACCAGTCGC CAATACCTGG CCCAATCCTG GCCCCCCAGA AGCCCAGTAG TGCTCATGCA 
CAGCAGCAGG CAGGGACAGT GCCTGGTGGG CCAACTCCTC CTCCCTCCAA CACCCTGCAC 
CCTCAGCAAC AGGGTGAGGA TGAAGATGAG ACATGGCGAC AAAGGAGAAA GCAGTCATCA 
ACCGAGATAT CTGCAGCAGT GGAGCGTGCC CGTCGACGCC GTGAAGAGGA GGAACGGAGG 
ATGCAGGAGG AGCGCCGTGC TGCCTGTGCA GAGAAGCTGA AAAGACTTGA TGAAAAACAG 
CAGCAACAGA CAATAAAACC CAGCAGCCCC AGTGACGCTC CAGCCAACAG CCCTAGTCCT 
TCTTTGTCTG CACATGCCTC ATCTCCCAAT GTCAGTCAGC CATCATCACC CTGCGTGGAC 
ACTGAAGAAC CACCTCTGTT ATCCGCTCAA GGAAGTGGTG CAACTCTAGG GCTTACTAAT 
AATAACAGAC AGAGAGCTGG TAGCAACAGC AGCCACGACT CCAACAATGA GTCCCAGCAG 
GTTCTGCAAC CTCAAGCCAC CCAGCATCCA CAATCCCAGG ATGTCCCAGG GCCTGGAGAT 
ATCAAGGAGG AGCATGTGGT GGGCGGTCCA CACATCCGCA GTACTAGAGT TGGAGATGAT 
TCAGTGAAGG TGGTTGAATG TGTTGGTGTA ACAGGACGGC AGGGAAGTGG TCCTTCTGGG 
CAAGGCTTTT CCAAGTATCA AAAATCGCTT CCTCCTCGCT TTCAGAGGCA ACAACAGGAG 
CAACTTCTCA AACAGCAACA GCAGTGGCAG CAGCAACAAC AGCAACATAA CCAGGTTGCT 
GCCCAAAATC AGTTGCAGTC ACAGCCTCAG AACCAGCAGG GCCCCGCTCC AGGTACCGCA 
CCACAGTCTG GGCCCAAACA GCCAACTCTT TATCCTCCTG GCTCAATGGG ACGTCCTCCA 
CCTCTTCCTA TGAACTTTGA TCCCCGCTGG ATGATGATGC CCTATATGGA CCCCCGAATG 
ATGCAGGGAC GCCCCCCTCC TATGGACTAC TACCCTCCAA GCATGCATCC TTCAGGGATG 
ATGAATCGAG AGCGTTCTGA TTCAGGTGGC TCTGGGTCAG ATCCCTTTGA TAGGCAGCAG 
CATGCTGGAC CCCCTCATTC CCATCGTGGT ACACCCCCCA TGGATCCCAA ACTGGCTTGG 
GGACCAGATG TTTTTCCAGG AGGAACAGAG GGACGAGGTC TAAGCTCCCC TTTACGTCAG 
AAAGCAGTTG AGGAAGAGGA GGCTGGAAAA GGTCAAAGAA GTGACACTCC TCCCGTTCGT 
ATGCGAGAAG GAGGCACAGC GTCTGTTCAG CAGCCTGGTG TGGCTCCCAG CTCTCCATCT 
GGCTCATCCA ACCAAACCCC ACCACCTCTG GGGATTCAAG TTGGGGGTGC AGGCCACCAT 
GCCCATCATC AACCCTTCTT GGATGGGCGA GGAGGTTACA GCAGCTACCC TGACAATGGA 
TCAAGAATGG GCTCCCACCT GCAGCAAAGG GCTAACAGTG GAGGGACACT GCATGGTTTT 
GGCCATCAGG AAGGTAGTCA GCCGAACCAA ATCTGGGGGA CTCCTCATCC TCATTATGAC 
CGTTCTGATC ACTCAGCCTT GGAGAGCAAC TCTCATCACC AACATCATGG TCCTCCCTCT 
CACGCCCCTC ATTTTTCTCT TCATCCTCAT AAGCAAGAGA ATGGCAGGGA GCGAGAAAAA 
ATGGTGGAGC CAAAGAAGAA TGACCCTTCA CCTCCACTCC ATCAGCCGTC CCTCTCCTCT 
TCCTGTTCAT CATCATCCTC GTCTTCCTCT GCCAGAGAAG ATGGCAGTGG AAAACAGTCT 
TTACATAATC AAGTTCCACC ACTGCATGAG CATGATACTG GATCCACTCA TGCTTCAGGA 
AGGAAGCAGG ACAAGACAGG AAATGCGCAT AATCAGCCCC CACAACATTC TGGGCAGCAG 
CACCACCCTA GAGCCAACCC TCGCAATCGG GAACACAAAA CTGAGACGCA CTGGGGCCCG 
AGGCCTGGTA GCAGTAGTGT GAGTGGAAGC TCTACTCACA ACAGAAAGAC AGGCTCTGGA 
ATTATTGCAA TTACAGAACC ATCTGGAAAC CAGGGTGCTG AGAACAAGTC CTTTAACCAA 
TCAGATGGCA TCACAGTCAA GCGGGCAGGT CCTATTAAGA GACCAGTTCT TAAAGAGATT 
AAAAGGGAGG GTGGTGAAGG AGGAGGAGAA AAGACTACTG GAGGCTCTGG TAAAGATAAA 
GAACAAGATG CAAGTCAAAC CACAACCAAA CAAGAACCTG CCACTGGGAA TCAAGTCATA 
TTAGCACCCA GTGGAAAAGA TGATGCCGTC TCATCAAATA AACCCAGAAC TGGTGGAAAG 
GAGCGAGGTA ACACTGGGGG TTCAGGCAAA GGATCCAAAA ATGGGGAAAG CTCCATGCAG 
AATTCTGGTT ATTCAGCCTC TCAGTCCAGG AGGGAAAGAG AACATTCACC TGAAAGAGGG 
AGCACAACCT ACCATTCGTC CTCATCCAGA GGTAGCCGAT CCAATCGTGG CAGAGGAGAG 
TTCTATGGTC GAGGCAGAGG CTATAGGGGC ACTTACGTGG GTAGTGCAGG TGGTGGCAAC 
CGTGGAAAGG CTGGTGGCAG GAACAGCAGG GATTATAGGC CAGCTGCCAA TAGTGGTTAC 
CACCATCCAT CTTCTAATCA AGATTACAAA AGAGAGGCAT CATCTGGGAG TGGCAGACAT 
GGCACATCTC AACCCAATCC TGGACGTGCT AGGAACCGTA GTGAGACTCG AAGCGAAGGC 
TCTGAATATG AAGAGGTGCC AAAGAGAAGG AGACAACGAG GATCTGAAAC AGGCAGTGAG 
AGTGCTGGAA GTGACCTTGC TCACTCTGAC AAGGAAGATC GCAAGCTCAA CACCAAGGCT 
GGAACAGTAG GAGAGAACCC TACAACTGGC CCTTCATCTT CTGCTTCAGT CAGAAACACT 
CAGACTAGAG TATTCACACC AAGGGGAGTA CCATCAAGAC GTGGTAGAGG TGGAGGTGGT 
GCTGGAGGGG GTCTCCATAG AAGCGGAGGT GGTGCTGGAG TGTCTGGTGG ACAAAGACCT 
GGACCTGGCT CTTCTTCCCA TGGCTGGTCG GCAAAGTCTG CAAATGTGCG AAAGCCACAG 
ACATCCTCAC AGCCACCCCC TGCTAAAGAT TTAAGCCGTG GAGTGAATGT TGACAAGAAG 
AAACCTGCAG AACAGAATCA GCCTCAAAGT CAAAGTGCAA ATCCCACCAC AACTGTTGCC 
TCTGTGCCTA CAACTATCCA ACAGGGGTCC ACTGAGAATG GAAATGTTGG AGCACCAATG 
TCTTCAACTA ATGCTCCATC AAATCCCGCT GGTGCACCTA CACAGTCTCT TCCTCTGCCT 
GCCCCAGATG GGCGGAACTT CCCTAACAGG GGATTTGAGC GCCCTCCTCG TCGTAGGCGC 
CATGGCCGAT CCCAGCATCA GCAGGACAAG CCTCCAAGAT TCCGCAGACT TAAACAAGAG 
CGAGAGAATG CAGCCCGTAT AAATGGAAGC AGTGGATTAA TTGAAGTCAT AGCTGCACAA 
CAGCAACGCA CTGCTCCACT GTTGCAAAAT TCCCTGCAAC AAGCCAACAG CACTCCCAAT 
GTACCCGATG CAGTAGTTGC AAATGCAAAT CACAGTGTGT CCACAACTCC TAACAGTAAT 
AATAATAGCA GCAACCTTGC AGGTGGAAAC CAAAATTTCA ATAGTCACCA CCACCATCAC 
TATCAGGGTA ACTCCCAGTC ACACCCTCAG CACCATCACA ACCATAACCC GGCCGGGGGC 
GCCAAATCTC CAGATGTCTC CAACCAGAAC TCTGACCAGG CAAATGAAGA ATGGGAAACT 
GCCTCAGAAA GCAGTGATTT CACAGAGTTT CGAGAGAGAG AGGGTGGAGG AGGTGGTAAA 
TCCTATTCCT CGCATCACCA TCATCACCAC CACCACCACC ATCCACCGGG AAGAGGCGGT 
GGAGGGGGCG GTGGTGGCGA CAGAGAGATG ACTGCTAAAG AATTAGCCTC TGGGAAGAGG 
AGCTTCTCCA GTCAGCGTCC AGGAATGGAG CGGCAGAACA GAAGGGTGAA TGCTGGTGGT 
GGTGGTGGAG GAAAAGGCCA GAGGGGTCCG CCCGGCACTG GTGGGCCAGT GGGTGGAGCT 
GGCAATGGCG GGGGTAATCG TGGTGAGAGG CGTGGCAACT GGCCTTCTCC CAAGAACAGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958504.2
CDS	259..6024
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQGPRPGLGGTREQPSPPPGPLLGQGPHSYYRQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	NM_201347.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201347.3

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CATGTATCCA CCATACCTGC CCTTTCCACC CCCTTATGGT 
CCACAGGGAC CTTACAGGTA CCCACCGCCA AATGAGGCTC CTAGGTTTTC TCGGAACCAG 
GGTGGAATAG GGCCAGATGG GCGACCCCCT GGAGGCCCCC GGGGTGAAGT GGTAAAACGC 
CCATCCATCC TCAAACAGGA TGACCTAAAA GAGCTGGATG AGCTGGACCA TGATGGAGAT 
GAGGGCTGGG CTGGAGCTCA AGAGGAAATT GACTACTCCG CGAAGTTGAA ATTCAGTGAC 
GATGAAGGAG AGGATGATGG GGAGGAGGAA CGGGGTGAAA ATAAAAATGG AACCCGGGAG 
TCAAGGGAAC AGCAGAGATC CCAAGATGGA CCTGTACCTG TTTCACGTTC CAGGGCATCC 
GACAGTGGAG GGGATTCGCG ACGCACCCCT CCTTCCAGTA CTGAAGATGG CTCTGTGTCC 
CCCTCCAGCA AGCCAGGCTG GGCCGAGGAG GGAGGTAGCA GCTGGAACAG TCAAGCAAAT 
GCCGGATCTT ATCAGGGGCC TAGGCCTGGA TTAGGTGGCA CTAGGGAGCA GCCCTCTCCC 
CCACCAGGTC CACTTCTTGG ACAAGGGCCC CACTCCTACT ACCGCCAGGA CCGGCCTTCA 
AACCAGTCGC CAATACCTGG CCCAATCCTG GCCCCCCAGA AGCCCAGTAG TGCTCATGCA 
CAGCAGCAGG CAGGGACAGT GCCTGGTGGG CCAACTCCTC CTCCCTCCAA CACCCTGCAC 
CCTCAGCAAC AGGGTGAGGA TGAAGATGAG ACATGGCGAC AAAGGAGAAA GCAGTCATCA 
ACCGAGATAT CTGCAGCAGT GGAGCGTGCC CGTCGACGCC GTGAAGAGGA GGAACGGAGG 
ATGCAGGAGG AGCGCCGTGC TGCCTGTGCA GAGAAGCTGA AAAGACTTGA TGAAAAACAG 
CAGCAACAGA CAATAAAACC CAGCAGCCCC AGTGACGCTC CAGCCAACAG CCCTAGTCCT 
TCTTTGTCTG CACATGCCTC ATCTCCCAAT GTCAGTCAGC CATCATCACC CTGCGTGGAC 
ACTGAAGAAC CACCTCTGTT ATCCGCTCAA GGAAGTGGTG CAACTCTAGG GCTTACTAAT 
AATAACAGAC AGAGAGCTGG TAGCAACAGC AGCCACGACT CCAACAATGA GTCCCAGCAG 
GTTCTGCAAC CTCAAGCCAC CCAGCATCCA CAATCCCAGG ATGTCCCAGG GCCTGGAGAT 
ATCAAGGAGG AGCATGTGGT GGGCGGTCCA CACATCCGCA GTACTAGAGT TGGAGATGAT 
TCAGTGAAGG TGGTTGAATG TGTTGGTGTA ACAGGACGGC AGGGAAGTGG TCCTTCTGGG 
CAAGGCTTTT CCAAGTATCA AAAATCGCTT CCTCCTCGCT TTCAGAGGCA ACAACAGGAG 
CAACTTCTCA AACAGCAACA GCAGTGGCAG CAGCAACAAC AGCAACATAA CCAGGTTGCT 
GCCCAAAATC AGTTGCAGTC ACAGCCTCAG AACCAGCAGG GCCCCGCTCC AGGTACCGCA 
CCACAGTCTG GGCCCAAACA GCCAACTCTT TATCCTCCTG GCTCAATGGG ACGTCCTCCA 
CCTCTTCCTA TGAACTTTGA TCCCCGCTGG ATGATGATGC CCTATATGGA CCCCCGAATG 
ATGCAGGGAC GCCCCCCTCC TATGGACTAC TACCCTCCAA GCATGCATCC TTCAGGGATG 
ATGAATCGAG AGCGTTCTGA TTCAGGTGGC TCTGGGTCAG ATCCCTTTGA TAGGCAGCAG 
CATGCTGGAC CCCCTCATTC CCATCGTGGT ACACCCCCCA TGGATCCCAA ACTGGCTTGG 
GGACCAGATG TTTTTCCAGG AGGAACAGAG GGACGAGGTC TAAGCTCCCC TTTACGTCAG 
AAAGCAGTTG AGGAAGAGGA GGCTGGAAAA GGTCAAAGAA GTGACACTCC TCCCGTTCGT 
ATGCGAGAAG GAGGCACAGC GTCTGTTCAG CAGCCTGGTG TGGCTCCCAG CTCTCCATCT 
GGCTCATCCA ACCAAACCCC ACCACCTCTG GGGATTCAAG TTGGGGGTGC AGGCCACCAT 
GCCCATCATC AACCCTTCTT GGATGGGCGA GGAGGTTACA GCAGCTACCC TGACAATGGA 
TCAAGAATGG GCTCCCACCT GCAGCAAAGG GCTAACAGTG GAGGGACACT GCATGGTTTT 
GGCCATCAGG AAGGTAGTCA GCCGAACCAA ATCTGGGGGA CTCCTCATCC TCATTATGAC 
CGTTCTGATC ACTCAGCCTT GGAGAGCAAC TCTCATCACC AACATCATGG TCCTCCCTCT 
CACGCCCCTC ATTTTTCTCT TCATCCTCAT AAGCAAGAGA ATGGCAGGGA GCGAGAAAAA 
ATGGTGGAGC CAAAGAAGAA TGACCCTTCA CCTCCACTCC ATCAGCCGTC CCTCTCCTCT 
TCCTGTTCAT CATCATCCTC GTCTTCCTCT GCCAGAGAAG ATGGCAGTGG AAAACAGTCT 
TTACATAATC AAGTTCCACC ACTGCATGAG CATGATACTG GATCCACTCA TGCTTCAGGA 
AGGAAGCAGG ACAAGACAGG AAATGCGCAT AATCAGCCCC CACAACATTC TGGGCAGCAG 
CACCACCCTA GAGCCAACCC TCGCAATCGG GAACACAAAA CTGAGACGCA CTGGGGCCCG 
AGGCCTGGTA GCAGTAGTGT GAGTGGAAGC TCTACTCACA ACAGAAAGAC AGGCTCTGGA 
ATTATTGCAA TTACAGAACC ATCTGGAAAC CAGGGTGCTG AGAACAAGTC CTTTAACCAA 
TCAGATGGCA TCACAGTCAA GCGGGCAGGT CCTATTAAGA GACCAGTTCT TAAAGAGATT 
AAAAGGGAGG GTGGTGAAGG AGGAGGAGAA AAGACTACTG GAGGCTCTGG TAAAGATAAA 
GAACAAGATG CAAGTCAAAC CACAACCAAA CAAGAACCTG CCACTGGGAA TCAAGTCATA 
TTAGCACCCA GTGGAAAAGA TGATGCCGTC TCATCAAATA AACCCAGAAC TGGTGGAAAG 
GAGCGAGGTA ACACTGGGGG TTCAGGCAAA GGATCCAAAA ATGGGGAAAG CTCCATGCAG 
AATTCTGGTT ATTCAGCCTC TCAGTCCAGG AGGGAAAGAG AACATTCACC TGAAAGAGGG 
AGCACAACCT ACCATTCGTC CTCATCCAGA GGTAGCCGAT CCAATCGTGG CAGAGGAGAG 
TTCTATGGTC GAGGCAGAGG CTATAGGGGC ACTTACGTGG GTAGTGCAGG TGGTGGCAAC 
CGTGGAAAGG CTGGTGGCAG GAACAGCAGG GATTATAGGC CAGCTGCCAA TAGTGGTTAC 
CACCATCCAT CTTCTAATCA AGATTACAAA AGAGAGGCAT CATCTGGGAG TGGCAGACAT 
GGCACATCTC AACCCAATCC TGGACGTGCT AGGAACCGTA GTGAGACTCG AAGCGAAGGC 
TCTGAATATG AAGAGGTGCC AAAGAGAAGG AGACAACGAG GATCTGAAAC AGGCAGTGAG 
AGTGCTGGAA GTGACCTTGC TCACTCTGAC AAGGAAGATC GCAAGCTCAA CACCAAGGCT 
GGAACAGTAG GAGAGAACCC TACAACTGGC CCTTCATCTT CTGCTTCAGT CAGAAACACT 
CAGACTAGAG TATTCACACC AAGGGGAGTA CCATCAAGAC GTGGTAGAGG TGGAGGTGGT 
GCTGGAGGGG GTCTCCATAG AAGCGGAGGT GGTGCTGGAG TGTCTGGTGG ACAAAGACCT 
GGACCTGGCT CTTCTTCCCA TGGCTGGTCG GCAAAGTCTG CAAATGTGCG AAAGCCACAG 
ACATCCTCAC AGCCACCCCC TGCTAAAGAT TTAAGCCGTG GAGTGAATGT TGACAAGAAG 
AAACCTGCAG AACAGAATCA GCCTCAAAGT CAAAGTGCAA ATCCCACCAC AACTGTTGCC 
TCTGTGCCTA CAACTATCCA ACAGGGGTCC ACTGAGAATG GAAATGTTGG AGCACCAATG 
TCTTCAACTA ATGCTCCATC AAATCCCGCT GGTGCACCTA CACAGTCTCT TCCTCTGCCT 
GCCCCAGATG GGCGGAACTT CCCTAACAGG GGATTTGAGC GCCCTCCTCG TCGTAGGCGC 
CATGGCCGAT CCCAGCATCA GCAGGACAAG CCTCCAAGAT TCCGCAGACT TAAACAAGAG 
CGAGAGAATG CAGCCCGTAT AAATGGAAGC AGTGGATTAA TTGAAGTCAT AGCTGCACAA 
CAGCAACGCA CTGCTCCACT GTTGCAAAAT TCCCTGCAAC AAGCCAACAG CACTCCCAAT 
GTACCCGATG CAGTAGTTGC AAATGCAAAT CACAGTGTGT CCACAACTCC TAACAGTAAT 
AATAATAGCA GCAACCTTGC AGGTGGAAAC CAAAATTTCA ATAGTCACCA CCACCATCAC 
TATCAGGGTA ACTCCCAGTC ACACCCTCAG CACCATCACA ACCATAACCC GGCCGGGGGC 
GCCAAATCTC CAGATGTCTC CAACCAGAAC TCTGACCAGG CAAATGAAGA ATGGGAAACT 
GCCTCAGAAA GCAGTGATTT CACAGAGTTT CGAGAGAGAG AGGGTGGAGG AGGTGGTAAA 
TCCTATTCCT CGCATCACCA TCATCACCAC CACCACCACC ATCCACCGGG AAGAGGCGGT 
GGAGGGGGCG GTGGTGGCGA CAGAGAGATG ACTGCTAAAG AATTAGCCTC TGGGAAGAGG 
AGCTTCTCCA GTCAGCGTCC AGGAATGGAG CGGCAGAACA GAAGGGTGAA TGCTGGTGGT 
GGTGGTGGAG GAAAAGGCCA GAGGGGTCCG CCCGGCACTG GTGGGCCAGT GGGTGGAGCT 
GGCAATGGCG GGGGTAATCG TGGTGAGAGG CGTGGCAACT GGCCTTCTCC CAAGAACAGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2a ( XM_005170015.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170072.1 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X3, mRNA.

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CloneID	ODa24122
Clone ID Related Accession (Same CDS sequence)	XM_005170015.4 , XM_005170015.3
Accession Version	XM_005170015.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein PRRC2A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170015.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGGGCCTAGG CCTGGATTAG GTGGCACTAG GGAGCAGCCC 
TCTCCCCCAC CAGGTCCACT TCTTGGACAA GGGCCCCACT CCTACTACCG CCAGGACCGG 
CCTTCAAACC AGTCGCCAAT ACCTGGCCCA ATCCTGGCCC CCCAGAAGCC CAGTAGTGCT 
CATGCACAGC AGCAGGCAGG GACAGTGCCT GGTGGGCCAA CTCCTCCTCC CTCCAACACC 
CTGCACCCTC AGCAACAGGG TGAGGATGAA GATGAGACAT GGCGACAAAG GAGAAAGCAG 
TCATCAACCG AGATATCTGC AGCAGTGGAG CGTGCCCGTC GACGCCGTGA AGAGGAGGAA 
CGGAGGATGC AGGAGGAGCG CCGTGCTGCC TGTGCAGAGA AGCTGAAAAG ACTTGATGAA 
AAACAGCAGC AACAGACAAT AAAACCCAGC AGCCCCAGTG ACGCTCCAGC CAACAGCCCT 
AGTCCTTCTT TGTCTGCACA TGCCTCATCT CCCAATGTCA GTCAGCCATC ATCACCCTGC 
GTGGACACTG AAGAACCACC TCTGTTATCC GCTCAAGGAA GTGGTGCAAC TCTAGGGCTT 
ACTAATAATA ACAGACAGAG AGCTGGTAGC AACAGCAGCC ACGACTCCAA CAATGAGTCC 
CAGCAGGTTC TGCAACCTCA AGCCACCCAG CATCCACAAT CCCAGGATGT CCCAGGGCCT 
GGAGATATCA AGGAGGAGCA TGTGGTGGGC GGTCCACACA TCCGCAGTAC TAGAGTTGGA 
GATGATTCAG TGAAGGTGGT TGAATGTGTT GGTGTAACAG GACGGCAGGG AAGTGGTCCT 
TCTGGGCAAG GCTTTTCCAA GTATCAAAAA TCGCTTCCTC CTCGCTTTCA GAGGCAACAA 
CAGGAGCAAC TTCTCAAACA GCAACAGCAG TGGCAGCAGC AACAACAGCA ACATAACCAG 
GTTGCTGCCC AAAATCAGTT GCAGTCACAG CCTCAGAACC AGCAGGGCCC CGCTCCAGGT 
ACCGCACCAC AGTCTGGGCC CAAACAGCCA ACTCTTTATC CTCCTGGCTC AATGGGACGT 
CCTCCACCTC TTCCTATGAA CTTTGATCCC CGCTGGATGA TGATGCCCTA TATGGACCCC 
CGAATGATGC AGGGACGCCC CCCTCCTATG GACTACTACC CTCCAAGCAT GCATCCTTCA 
GGGATGATGA ATCGAGAGCG TTCTGATTCA GGTGGCTCTG GGTCAGATCC CTTTGATAGG 
CAGCAGCATG CTGGACCCCC TCATTCCCAT CGTGGTACAC CCCCCATGGA TCCCAAACTG 
GCTTGGGGAC CAGATGTTTT TCCAGGAGGA ACAGAGGGAC GAGGTCTAAG CTCCCCTTTA 
CGTCAGAAAG CAGTTGAGGA AGAGGAGGCT GGAAAAGGTC AAAGAAGTGA CACTCCTCCC 
GTTCGTATGC GAGAAGGAGG CACAGCGTCT GTTCAGCAGC CTGGTGTGGC TCCCAGCTCT 
CCATCTGGCT CATCCAACCA AACCCCACCA CCTCTGGGGA TTCAAGTTGG GGGTGCAGGC 
CACCATGCCC ATCATCAACC CTTCTTGGAT GGGCGAGGAG GTTACAGCAG CTACCCTGAC 
AATGGATCAA GAATGGGCTC CCACCTGCAG CAAAGGGCTA ACAGTGGAGG GACACTGCAT 
GGTTTTGGCC ATCAGGAAGG TAGTCAGCCG AACCAAATCT GGGGGACTCC TCATCCTCAT 
TATGACCGTT CTGATCACTC AGCCTTGGAG AGCAACTCTC ATCACCAACA TCATGGTCCT 
CCCTCTCACG CCCCTCATTT TTCTCTTCAT CCTCATAAGC AAGAGAATGG CAGGGAGCGA 
GAAAAAATGG TGGAGCCAAA GAAGAATGAC CCTTCACCTC CACTCCATCA GCCGTCCCTC 
TCCTCTTCCT GTTCATCATC ATCCTCGTCT TCCTCTGCCA GAGAAGATGG CAGTGGAAAA 
CAGTCTTTAC ATAATCAAGT TCCACCACTG CATGAGCATG ATACTGGATC CACTCATGCT 
TCAGGAAGGA AGCAGGACAA GACAGGAAAT GCGCATAATC AGCCCCCACA ACATTCTGGG 
CAGCAGCACC ACCCTAGAGC CAACCCTCGC AATCGGGAAC ACAAAACTGA GACGCACTGG 
GGCCCGAGGC CTGGTAGCAG TAGTGTGAGT GGAAGCTCTA CTCACAACAG AAAGACAGGC 
TCTGGAATTA TTGCAATTAC AGAACCATCT GGAAACCAGG GTGCTGAGAA CAAGTCCTTT 
AACCAATCAG ATGGCATCAC AGTCAAGCGG GCAGGTCCTA TTAAGAGACC AGTTCTTAAA 
GAGATTAAAA GGGAGGGTGG TGAAGGAGGA GGAGAAAAGA CTACTGGAGG CTCTGGTAAA 
GATAAAGAAC AAGATGCAAG TCAAACCACA ACCAAACAAG AACCTGCCAC TGGGAATCAA 
GTCATATTAG CACCCAGTGG AAAAGATGAT GCCGTCTCAT CAAATAAACC CAGAACTGGT 
GGAAAGGAGC GAGGTAACAC TGGGGGTTCA GGCAAAGGAT CCAAAAATGG GGAAAGCTCC 
ATGCAGAATT CTGGTTATTC AGCCTCTCAG TCCAGGAGGG AAAGAGAACA TTCACCTGAA 
AGAGGGAGCA CAACCTACCA TTCGTCCTCA TCCAGAGGTA GCCGATCCAA TCGTGGCAGA 
GGAGAGTTCT ATGGTCGAGG CAGAGGCTAT AGGGGCACTT ACGTGGGTAG TGCAGGTGGT 
GGCAACCGTG GAAAGGCTGG TGGCAGGAAC AGCAGGGATT ATAGGCCAGC TGCCAATAGT 
GGTTACCACC ATCCATCTTC TAATCAAGAT TACAAAAGAG AGGCATCATC TGGGAGTGGC 
AGACATGGCA CATCTCAACC CAATCCTGGA CGTGCTAGGA ACCGTAGTGA GACTCGAAGC 
GAAGGCTCTG AATATGAAGA GGTGCCAAAG AGAAGGAGAC AACGAGGATC TGAAACAGGC 
AGTGAGAGTG CTGGAAGTGA CCTTGCTCAC TCTGACAAGG AAGATCGCAA GCTCAACACC 
AAGGCTGGAA CAGTAGGAGA GAACCCTACA ACTGGCCCTT CATCTTCTGC TTCAGTCAGA 
AACACTCAGA CTAGAGTATT CACACCAAGG GGAGTACCAT CAAGACGTGG TAGAGGTGGA 
GGTGGTGCTG GAGGGGGTCT CCATAGAAGC GGAGGTGGTG CTGGAGTGTC TGGTGGACAA 
AGACCTGGAC CTGGCTCTTC TTCCCATGGC TGGTCGGCAA AGTCTGCAAA TGTGCGAAAG 
CCACAGACAT CCTCACAGCC ACCCCCTGCT AAAGATTTAA GCCGTGGAGT GAATGTTGAC 
AAGAAGAAAC CTGCAGAACA GAATCAGCCT CAAAGTCAAA GTGCAAATCC CACCACAACT 
GTTGCCTCTG TGCCTACAAC TATCCAACAG GGGTCCACTG AGAATGGAAA TGTTGGAGCA 
CCAATGTCTT CAACTAATGC TCCATCAAAT CCCGCTGGTG CACCTACACA GTCTCTTCCT 
CTGCCTGCCC CAGATGGGCG GAACTTCCCT AACAGGGGAT TTGAGCGCCC TCCTCGTCGT 
AGGCGCCATG GCCGATCCCA GCATCAGCAG GACAAGCCTC CAAGATTCCG CAGACTTAAA 
CAAGAGCGAG AGAATGCAGC CCGTATAAAT GGAAGCAGTG GATTAATTGA AGTCATAGCT 
GCACAACAGC AACGCACTGC TCCACTGTTG CAAAATTCCC TGCAACAAGC CAACAGCACT 
CCCAATGTAC CCGATGCAGT AGTTGCAAAT GCAAATCACA GTGTGTCCAC AACTCCTAAC 
AGTAATAATA ATAGCAGCAA CCTTGCAGGT GGAAACCAAA ATTTCAATAG TCACCACCAC 
CATCACTATC AGGGTAACTC CCAGTCACAC CCTCAGCACC ATCACAACCA TAACCCGGCC 
GGGGGCGCCA AATCTCCAGA TGTCTCCAAC CAGAACTCTG ACCAGGCAAA TGAAGAATGG 
GAAACTGCCT CAGAAAGCAG TGATTTCACA GAGTTTCGAG AGAGAGAGGG TGGAGGAGGT 
GGTAAATCCT ATTCCTCGCA TCACCATCAT CACCACCACC ACCACCATCC ACCGGGAAGA 
GGCGGTGGAG GGGGCGGTGG TGGCGACAGA GAGATGACTG CTAAAGAATT AGCCTCTGGG 
AAGAGGAGCT TCTCCAGTCA GCGTCCAGGA ATGGAGCGGC AGAACAGAAG GGTGAATGCT 
GGTGGTGGTG GTGGAGGAAA AGGCCAGAGG GGTCCGCCCG GCACTGGTGG GCCAGTGGGT 
GGAGCTGGCA ATGGCGGGGG TAATCGTGGT GAGAGGCGTG GCAACTGGCC TTCTCCCAAG 
AACAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170072.1
CDS	331..6102
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFVSMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQGPRPGLGGTREQPSPPPGPLLGQGPHSYYRQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	XM_005170015.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170015.4

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGGGCCTAGG CCTGGATTAG GTGGCACTAG GGAGCAGCCC 
TCTCCCCCAC CAGGTCCACT TCTTGGACAA GGGCCCCACT CCTACTACCG CCAGGACCGG 
CCTTCAAACC AGTCGCCAAT ACCTGGCCCA ATCCTGGCCC CCCAGAAGCC CAGTAGTGCT 
CATGCACAGC AGCAGGCAGG GACAGTGCCT GGTGGGCCAA CTCCTCCTCC CTCCAACACC 
CTGCACCCTC AGCAACAGGG TGAGGATGAA GATGAGACAT GGCGACAAAG GAGAAAGCAG 
TCATCAACCG AGATATCTGC AGCAGTGGAG CGTGCCCGTC GACGCCGTGA AGAGGAGGAA 
CGGAGGATGC AGGAGGAGCG CCGTGCTGCC TGTGCAGAGA AGCTGAAAAG ACTTGATGAA 
AAACAGCAGC AACAGACAAT AAAACCCAGC AGCCCCAGTG ACGCTCCAGC CAACAGCCCT 
AGTCCTTCTT TGTCTGCACA TGCCTCATCT CCCAATGTCA GTCAGCCATC ATCACCCTGC 
GTGGACACTG AAGAACCACC TCTGTTATCC GCTCAAGGAA GTGGTGCAAC TCTAGGGCTT 
ACTAATAATA ACAGACAGAG AGCTGGTAGC AACAGCAGCC ACGACTCCAA CAATGAGTCC 
CAGCAGGTTC TGCAACCTCA AGCCACCCAG CATCCACAAT CCCAGGATGT CCCAGGGCCT 
GGAGATATCA AGGAGGAGCA TGTGGTGGGC GGTCCACACA TCCGCAGTAC TAGAGTTGGA 
GATGATTCAG TGAAGGTGGT TGAATGTGTT GGTGTAACAG GACGGCAGGG AAGTGGTCCT 
TCTGGGCAAG GCTTTTCCAA GTATCAAAAA TCGCTTCCTC CTCGCTTTCA GAGGCAACAA 
CAGGAGCAAC TTCTCAAACA GCAACAGCAG TGGCAGCAGC AACAACAGCA ACATAACCAG 
GTTGCTGCCC AAAATCAGTT GCAGTCACAG CCTCAGAACC AGCAGGGCCC CGCTCCAGGT 
ACCGCACCAC AGTCTGGGCC CAAACAGCCA ACTCTTTATC CTCCTGGCTC AATGGGACGT 
CCTCCACCTC TTCCTATGAA CTTTGATCCC CGCTGGATGA TGATGCCCTA TATGGACCCC 
CGAATGATGC AGGGACGCCC CCCTCCTATG GACTACTACC CTCCAAGCAT GCATCCTTCA 
GGGATGATGA ATCGAGAGCG TTCTGATTCA GGTGGCTCTG GGTCAGATCC CTTTGATAGG 
CAGCAGCATG CTGGACCCCC TCATTCCCAT CGTGGTACAC CCCCCATGGA TCCCAAACTG 
GCTTGGGGAC CAGATGTTTT TCCAGGAGGA ACAGAGGGAC GAGGTCTAAG CTCCCCTTTA 
CGTCAGAAAG CAGTTGAGGA AGAGGAGGCT GGAAAAGGTC AAAGAAGTGA CACTCCTCCC 
GTTCGTATGC GAGAAGGAGG CACAGCGTCT GTTCAGCAGC CTGGTGTGGC TCCCAGCTCT 
CCATCTGGCT CATCCAACCA AACCCCACCA CCTCTGGGGA TTCAAGTTGG GGGTGCAGGC 
CACCATGCCC ATCATCAACC CTTCTTGGAT GGGCGAGGAG GTTACAGCAG CTACCCTGAC 
AATGGATCAA GAATGGGCTC CCACCTGCAG CAAAGGGCTA ACAGTGGAGG GACACTGCAT 
GGTTTTGGCC ATCAGGAAGG TAGTCAGCCG AACCAAATCT GGGGGACTCC TCATCCTCAT 
TATGACCGTT CTGATCACTC AGCCTTGGAG AGCAACTCTC ATCACCAACA TCATGGTCCT 
CCCTCTCACG CCCCTCATTT TTCTCTTCAT CCTCATAAGC AAGAGAATGG CAGGGAGCGA 
GAAAAAATGG TGGAGCCAAA GAAGAATGAC CCTTCACCTC CACTCCATCA GCCGTCCCTC 
TCCTCTTCCT GTTCATCATC ATCCTCGTCT TCCTCTGCCA GAGAAGATGG CAGTGGAAAA 
CAGTCTTTAC ATAATCAAGT TCCACCACTG CATGAGCATG ATACTGGATC CACTCATGCT 
TCAGGAAGGA AGCAGGACAA GACAGGAAAT GCGCATAATC AGCCCCCACA ACATTCTGGG 
CAGCAGCACC ACCCTAGAGC CAACCCTCGC AATCGGGAAC ACAAAACTGA GACGCACTGG 
GGCCCGAGGC CTGGTAGCAG TAGTGTGAGT GGAAGCTCTA CTCACAACAG AAAGACAGGC 
TCTGGAATTA TTGCAATTAC AGAACCATCT GGAAACCAGG GTGCTGAGAA CAAGTCCTTT 
AACCAATCAG ATGGCATCAC AGTCAAGCGG GCAGGTCCTA TTAAGAGACC AGTTCTTAAA 
GAGATTAAAA GGGAGGGTGG TGAAGGAGGA GGAGAAAAGA CTACTGGAGG CTCTGGTAAA 
GATAAAGAAC AAGATGCAAG TCAAACCACA ACCAAACAAG AACCTGCCAC TGGGAATCAA 
GTCATATTAG CACCCAGTGG AAAAGATGAT GCCGTCTCAT CAAATAAACC CAGAACTGGT 
GGAAAGGAGC GAGGTAACAC TGGGGGTTCA GGCAAAGGAT CCAAAAATGG GGAAAGCTCC 
ATGCAGAATT CTGGTTATTC AGCCTCTCAG TCCAGGAGGG AAAGAGAACA TTCACCTGAA 
AGAGGGAGCA CAACCTACCA TTCGTCCTCA TCCAGAGGTA GCCGATCCAA TCGTGGCAGA 
GGAGAGTTCT ATGGTCGAGG CAGAGGCTAT AGGGGCACTT ACGTGGGTAG TGCAGGTGGT 
GGCAACCGTG GAAAGGCTGG TGGCAGGAAC AGCAGGGATT ATAGGCCAGC TGCCAATAGT 
GGTTACCACC ATCCATCTTC TAATCAAGAT TACAAAAGAG AGGCATCATC TGGGAGTGGC 
AGACATGGCA CATCTCAACC CAATCCTGGA CGTGCTAGGA ACCGTAGTGA GACTCGAAGC 
GAAGGCTCTG AATATGAAGA GGTGCCAAAG AGAAGGAGAC AACGAGGATC TGAAACAGGC 
AGTGAGAGTG CTGGAAGTGA CCTTGCTCAC TCTGACAAGG AAGATCGCAA GCTCAACACC 
AAGGCTGGAA CAGTAGGAGA GAACCCTACA ACTGGCCCTT CATCTTCTGC TTCAGTCAGA 
AACACTCAGA CTAGAGTATT CACACCAAGG GGAGTACCAT CAAGACGTGG TAGAGGTGGA 
GGTGGTGCTG GAGGGGGTCT CCATAGAAGC GGAGGTGGTG CTGGAGTGTC TGGTGGACAA 
AGACCTGGAC CTGGCTCTTC TTCCCATGGC TGGTCGGCAA AGTCTGCAAA TGTGCGAAAG 
CCACAGACAT CCTCACAGCC ACCCCCTGCT AAAGATTTAA GCCGTGGAGT GAATGTTGAC 
AAGAAGAAAC CTGCAGAACA GAATCAGCCT CAAAGTCAAA GTGCAAATCC CACCACAACT 
GTTGCCTCTG TGCCTACAAC TATCCAACAG GGGTCCACTG AGAATGGAAA TGTTGGAGCA 
CCAATGTCTT CAACTAATGC TCCATCAAAT CCCGCTGGTG CACCTACACA GTCTCTTCCT 
CTGCCTGCCC CAGATGGGCG GAACTTCCCT AACAGGGGAT TTGAGCGCCC TCCTCGTCGT 
AGGCGCCATG GCCGATCCCA GCATCAGCAG GACAAGCCTC CAAGATTCCG CAGACTTAAA 
CAAGAGCGAG AGAATGCAGC CCGTATAAAT GGAAGCAGTG GATTAATTGA AGTCATAGCT 
GCACAACAGC AACGCACTGC TCCACTGTTG CAAAATTCCC TGCAACAAGC CAACAGCACT 
CCCAATGTAC CCGATGCAGT AGTTGCAAAT GCAAATCACA GTGTGTCCAC AACTCCTAAC 
AGTAATAATA ATAGCAGCAA CCTTGCAGGT GGAAACCAAA ATTTCAATAG TCACCACCAC 
CATCACTATC AGGGTAACTC CCAGTCACAC CCTCAGCACC ATCACAACCA TAACCCGGCC 
GGGGGCGCCA AATCTCCAGA TGTCTCCAAC CAGAACTCTG ACCAGGCAAA TGAAGAATGG 
GAAACTGCCT CAGAAAGCAG TGATTTCACA GAGTTTCGAG AGAGAGAGGG TGGAGGAGGT 
GGTAAATCCT ATTCCTCGCA TCACCATCAT CACCACCACC ACCACCATCC ACCGGGAAGA 
GGCGGTGGAG GGGGCGGTGG TGGCGACAGA GAGATGACTG CTAAAGAATT AGCCTCTGGG 
AAGAGGAGCT TCTCCAGTCA GCGTCCAGGA ATGGAGCGGC AGAACAGAAG GGTGAATGCT 
GGTGGTGGTG GTGGAGGAAA AGGCCAGAGG GGTCCGCCCG GCACTGGTGG GCCAGTGGGT 
GGAGCTGGCA ATGGCGGGGG TAATCGTGGT GAGAGGCGTG GCAACTGGCC TTCTCCCAAG 
AACAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2a ( XM_005170015.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170072.1 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24122
Clone ID Related Accession (Same CDS sequence)	XM_005170015.4 , XM_005170015.3
Accession Version	XM_005170015.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170015.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGGGCCTAGG CCTGGATTAG GTGGCACTAG GGAGCAGCCC 
TCTCCCCCAC CAGGTCCACT TCTTGGACAA GGGCCCCACT CCTACTACCG CCAGGACCGG 
CCTTCAAACC AGTCGCCAAT ACCTGGCCCA ATCCTGGCCC CCCAGAAGCC CAGTAGTGCT 
CATGCACAGC AGCAGGCAGG GACAGTGCCT GGTGGGCCAA CTCCTCCTCC CTCCAACACC 
CTGCACCCTC AGCAACAGGG TGAGGATGAA GATGAGACAT GGCGACAAAG GAGAAAGCAG 
TCATCAACCG AGATATCTGC AGCAGTGGAG CGTGCCCGTC GACGCCGTGA AGAGGAGGAA 
CGGAGGATGC AGGAGGAGCG CCGTGCTGCC TGTGCAGAGA AGCTGAAAAG ACTTGATGAA 
AAACAGCAGC AACAGACAAT AAAACCCAGC AGCCCCAGTG ACGCTCCAGC CAACAGCCCT 
AGTCCTTCTT TGTCTGCACA TGCCTCATCT CCCAATGTCA GTCAGCCATC ATCACCCTGC 
GTGGACACTG AAGAACCACC TCTGTTATCC GCTCAAGGAA GTGGTGCAAC TCTAGGGCTT 
ACTAATAATA ACAGACAGAG AGCTGGTAGC AACAGCAGCC ACGACTCCAA CAATGAGTCC 
CAGCAGGTTC TGCAACCTCA AGCCACCCAG CATCCACAAT CCCAGGATGT CCCAGGGCCT 
GGAGATATCA AGGAGGAGCA TGTGGTGGGC GGTCCACACA TCCGCAGTAC TAGAGTTGGA 
GATGATTCAG TGAAGGTGGT TGAATGTGTT GGTGTAACAG GACGGCAGGG AAGTGGTCCT 
TCTGGGCAAG GCTTTTCCAA GTATCAAAAA TCGCTTCCTC CTCGCTTTCA GAGGCAACAA 
CAGGAGCAAC TTCTCAAACA GCAACAGCAG TGGCAGCAGC AACAACAGCA ACATAACCAG 
GTTGCTGCCC AAAATCAGTT GCAGTCACAG CCTCAGAACC AGCAGGGCCC CGCTCCAGGT 
ACCGCACCAC AGTCTGGGCC CAAACAGCCA ACTCTTTATC CTCCTGGCTC AATGGGACGT 
CCTCCACCTC TTCCTATGAA CTTTGATCCC CGCTGGATGA TGATGCCCTA TATGGACCCC 
CGAATGATGC AGGGACGCCC CCCTCCTATG GACTACTACC CTCCAAGCAT GCATCCTTCA 
GGGATGATGA ATCGAGAGCG TTCTGATTCA GGTGGCTCTG GGTCAGATCC CTTTGATAGG 
CAGCAGCATG CTGGACCCCC TCATTCCCAT CGTGGTACAC CCCCCATGGA TCCCAAACTG 
GCTTGGGGAC CAGATGTTTT TCCAGGAGGA ACAGAGGGAC GAGGTCTAAG CTCCCCTTTA 
CGTCAGAAAG CAGTTGAGGA AGAGGAGGCT GGAAAAGGTC AAAGAAGTGA CACTCCTCCC 
GTTCGTATGC GAGAAGGAGG CACAGCGTCT GTTCAGCAGC CTGGTGTGGC TCCCAGCTCT 
CCATCTGGCT CATCCAACCA AACCCCACCA CCTCTGGGGA TTCAAGTTGG GGGTGCAGGC 
CACCATGCCC ATCATCAACC CTTCTTGGAT GGGCGAGGAG GTTACAGCAG CTACCCTGAC 
AATGGATCAA GAATGGGCTC CCACCTGCAG CAAAGGGCTA ACAGTGGAGG GACACTGCAT 
GGTTTTGGCC ATCAGGAAGG TAGTCAGCCG AACCAAATCT GGGGGACTCC TCATCCTCAT 
TATGACCGTT CTGATCACTC AGCCTTGGAG AGCAACTCTC ATCACCAACA TCATGGTCCT 
CCCTCTCACG CCCCTCATTT TTCTCTTCAT CCTCATAAGC AAGAGAATGG CAGGGAGCGA 
GAAAAAATGG TGGAGCCAAA GAAGAATGAC CCTTCACCTC CACTCCATCA GCCGTCCCTC 
TCCTCTTCCT GTTCATCATC ATCCTCGTCT TCCTCTGCCA GAGAAGATGG CAGTGGAAAA 
CAGTCTTTAC ATAATCAAGT TCCACCACTG CATGAGCATG ATACTGGATC CACTCATGCT 
TCAGGAAGGA AGCAGGACAA GACAGGAAAT GCGCATAATC AGCCCCCACA ACATTCTGGG 
CAGCAGCACC ACCCTAGAGC CAACCCTCGC AATCGGGAAC ACAAAACTGA GACGCACTGG 
GGCCCGAGGC CTGGTAGCAG TAGTGTGAGT GGAAGCTCTA CTCACAACAG AAAGACAGGC 
TCTGGAATTA TTGCAATTAC AGAACCATCT GGAAACCAGG GTGCTGAGAA CAAGTCCTTT 
AACCAATCAG ATGGCATCAC AGTCAAGCGG GCAGGTCCTA TTAAGAGACC AGTTCTTAAA 
GAGATTAAAA GGGAGGGTGG TGAAGGAGGA GGAGAAAAGA CTACTGGAGG CTCTGGTAAA 
GATAAAGAAC AAGATGCAAG TCAAACCACA ACCAAACAAG AACCTGCCAC TGGGAATCAA 
GTCATATTAG CACCCAGTGG AAAAGATGAT GCCGTCTCAT CAAATAAACC CAGAACTGGT 
GGAAAGGAGC GAGGTAACAC TGGGGGTTCA GGCAAAGGAT CCAAAAATGG GGAAAGCTCC 
ATGCAGAATT CTGGTTATTC AGCCTCTCAG TCCAGGAGGG AAAGAGAACA TTCACCTGAA 
AGAGGGAGCA CAACCTACCA TTCGTCCTCA TCCAGAGGTA GCCGATCCAA TCGTGGCAGA 
GGAGAGTTCT ATGGTCGAGG CAGAGGCTAT AGGGGCACTT ACGTGGGTAG TGCAGGTGGT 
GGCAACCGTG GAAAGGCTGG TGGCAGGAAC AGCAGGGATT ATAGGCCAGC TGCCAATAGT 
GGTTACCACC ATCCATCTTC TAATCAAGAT TACAAAAGAG AGGCATCATC TGGGAGTGGC 
AGACATGGCA CATCTCAACC CAATCCTGGA CGTGCTAGGA ACCGTAGTGA GACTCGAAGC 
GAAGGCTCTG AATATGAAGA GGTGCCAAAG AGAAGGAGAC AACGAGGATC TGAAACAGGC 
AGTGAGAGTG CTGGAAGTGA CCTTGCTCAC TCTGACAAGG AAGATCGCAA GCTCAACACC 
AAGGCTGGAA CAGTAGGAGA GAACCCTACA ACTGGCCCTT CATCTTCTGC TTCAGTCAGA 
AACACTCAGA CTAGAGTATT CACACCAAGG GGAGTACCAT CAAGACGTGG TAGAGGTGGA 
GGTGGTGCTG GAGGGGGTCT CCATAGAAGC GGAGGTGGTG CTGGAGTGTC TGGTGGACAA 
AGACCTGGAC CTGGCTCTTC TTCCCATGGC TGGTCGGCAA AGTCTGCAAA TGTGCGAAAG 
CCACAGACAT CCTCACAGCC ACCCCCTGCT AAAGATTTAA GCCGTGGAGT GAATGTTGAC 
AAGAAGAAAC CTGCAGAACA GAATCAGCCT CAAAGTCAAA GTGCAAATCC CACCACAACT 
GTTGCCTCTG TGCCTACAAC TATCCAACAG GGGTCCACTG AGAATGGAAA TGTTGGAGCA 
CCAATGTCTT CAACTAATGC TCCATCAAAT CCCGCTGGTG CACCTACACA GTCTCTTCCT 
CTGCCTGCCC CAGATGGGCG GAACTTCCCT AACAGGGGAT TTGAGCGCCC TCCTCGTCGT 
AGGCGCCATG GCCGATCCCA GCATCAGCAG GACAAGCCTC CAAGATTCCG CAGACTTAAA 
CAAGAGCGAG AGAATGCAGC CCGTATAAAT GGAAGCAGTG GATTAATTGA AGTCATAGCT 
GCACAACAGC AACGCACTGC TCCACTGTTG CAAAATTCCC TGCAACAAGC CAACAGCACT 
CCCAATGTAC CCGATGCAGT AGTTGCAAAT GCAAATCACA GTGTGTCCAC AACTCCTAAC 
AGTAATAATA ATAGCAGCAA CCTTGCAGGT GGAAACCAAA ATTTCAATAG TCACCACCAC 
CATCACTATC AGGGTAACTC CCAGTCACAC CCTCAGCACC ATCACAACCA TAACCCGGCC 
GGGGGCGCCA AATCTCCAGA TGTCTCCAAC CAGAACTCTG ACCAGGCAAA TGAAGAATGG 
GAAACTGCCT CAGAAAGCAG TGATTTCACA GAGTTTCGAG AGAGAGAGGG TGGAGGAGGT 
GGTAAATCCT ATTCCTCGCA TCACCATCAT CACCACCACC ACCACCATCC ACCGGGAAGA 
GGCGGTGGAG GGGGCGGTGG TGGCGACAGA GAGATGACTG CTAAAGAATT AGCCTCTGGG 
AAGAGGAGCT TCTCCAGTCA GCGTCCAGGA ATGGAGCGGC AGAACAGAAG GGTGAATGCT 
GGTGGTGGTG GTGGAGGAAA AGGCCAGAGG GGTCCGCCCG GCACTGGTGG GCCAGTGGGT 
GGAGCTGGCA ATGGCGGGGG TAATCGTGGT GAGAGGCGTG GCAACTGGCC TTCTCCCAAG 
AACAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170072.1
CDS	321..6092
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFVSMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQGPRPGLGGTREQPSPPPGPLLGQGPHSYYRQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	XM_005170015.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170015.3

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGGGCCTAGG CCTGGATTAG GTGGCACTAG GGAGCAGCCC 
TCTCCCCCAC CAGGTCCACT TCTTGGACAA GGGCCCCACT CCTACTACCG CCAGGACCGG 
CCTTCAAACC AGTCGCCAAT ACCTGGCCCA ATCCTGGCCC CCCAGAAGCC CAGTAGTGCT 
CATGCACAGC AGCAGGCAGG GACAGTGCCT GGTGGGCCAA CTCCTCCTCC CTCCAACACC 
CTGCACCCTC AGCAACAGGG TGAGGATGAA GATGAGACAT GGCGACAAAG GAGAAAGCAG 
TCATCAACCG AGATATCTGC AGCAGTGGAG CGTGCCCGTC GACGCCGTGA AGAGGAGGAA 
CGGAGGATGC AGGAGGAGCG CCGTGCTGCC TGTGCAGAGA AGCTGAAAAG ACTTGATGAA 
AAACAGCAGC AACAGACAAT AAAACCCAGC AGCCCCAGTG ACGCTCCAGC CAACAGCCCT 
AGTCCTTCTT TGTCTGCACA TGCCTCATCT CCCAATGTCA GTCAGCCATC ATCACCCTGC 
GTGGACACTG AAGAACCACC TCTGTTATCC GCTCAAGGAA GTGGTGCAAC TCTAGGGCTT 
ACTAATAATA ACAGACAGAG AGCTGGTAGC AACAGCAGCC ACGACTCCAA CAATGAGTCC 
CAGCAGGTTC TGCAACCTCA AGCCACCCAG CATCCACAAT CCCAGGATGT CCCAGGGCCT 
GGAGATATCA AGGAGGAGCA TGTGGTGGGC GGTCCACACA TCCGCAGTAC TAGAGTTGGA 
GATGATTCAG TGAAGGTGGT TGAATGTGTT GGTGTAACAG GACGGCAGGG AAGTGGTCCT 
TCTGGGCAAG GCTTTTCCAA GTATCAAAAA TCGCTTCCTC CTCGCTTTCA GAGGCAACAA 
CAGGAGCAAC TTCTCAAACA GCAACAGCAG TGGCAGCAGC AACAACAGCA ACATAACCAG 
GTTGCTGCCC AAAATCAGTT GCAGTCACAG CCTCAGAACC AGCAGGGCCC CGCTCCAGGT 
ACCGCACCAC AGTCTGGGCC CAAACAGCCA ACTCTTTATC CTCCTGGCTC AATGGGACGT 
CCTCCACCTC TTCCTATGAA CTTTGATCCC CGCTGGATGA TGATGCCCTA TATGGACCCC 
CGAATGATGC AGGGACGCCC CCCTCCTATG GACTACTACC CTCCAAGCAT GCATCCTTCA 
GGGATGATGA ATCGAGAGCG TTCTGATTCA GGTGGCTCTG GGTCAGATCC CTTTGATAGG 
CAGCAGCATG CTGGACCCCC TCATTCCCAT CGTGGTACAC CCCCCATGGA TCCCAAACTG 
GCTTGGGGAC CAGATGTTTT TCCAGGAGGA ACAGAGGGAC GAGGTCTAAG CTCCCCTTTA 
CGTCAGAAAG CAGTTGAGGA AGAGGAGGCT GGAAAAGGTC AAAGAAGTGA CACTCCTCCC 
GTTCGTATGC GAGAAGGAGG CACAGCGTCT GTTCAGCAGC CTGGTGTGGC TCCCAGCTCT 
CCATCTGGCT CATCCAACCA AACCCCACCA CCTCTGGGGA TTCAAGTTGG GGGTGCAGGC 
CACCATGCCC ATCATCAACC CTTCTTGGAT GGGCGAGGAG GTTACAGCAG CTACCCTGAC 
AATGGATCAA GAATGGGCTC CCACCTGCAG CAAAGGGCTA ACAGTGGAGG GACACTGCAT 
GGTTTTGGCC ATCAGGAAGG TAGTCAGCCG AACCAAATCT GGGGGACTCC TCATCCTCAT 
TATGACCGTT CTGATCACTC AGCCTTGGAG AGCAACTCTC ATCACCAACA TCATGGTCCT 
CCCTCTCACG CCCCTCATTT TTCTCTTCAT CCTCATAAGC AAGAGAATGG CAGGGAGCGA 
GAAAAAATGG TGGAGCCAAA GAAGAATGAC CCTTCACCTC CACTCCATCA GCCGTCCCTC 
TCCTCTTCCT GTTCATCATC ATCCTCGTCT TCCTCTGCCA GAGAAGATGG CAGTGGAAAA 
CAGTCTTTAC ATAATCAAGT TCCACCACTG CATGAGCATG ATACTGGATC CACTCATGCT 
TCAGGAAGGA AGCAGGACAA GACAGGAAAT GCGCATAATC AGCCCCCACA ACATTCTGGG 
CAGCAGCACC ACCCTAGAGC CAACCCTCGC AATCGGGAAC ACAAAACTGA GACGCACTGG 
GGCCCGAGGC CTGGTAGCAG TAGTGTGAGT GGAAGCTCTA CTCACAACAG AAAGACAGGC 
TCTGGAATTA TTGCAATTAC AGAACCATCT GGAAACCAGG GTGCTGAGAA CAAGTCCTTT 
AACCAATCAG ATGGCATCAC AGTCAAGCGG GCAGGTCCTA TTAAGAGACC AGTTCTTAAA 
GAGATTAAAA GGGAGGGTGG TGAAGGAGGA GGAGAAAAGA CTACTGGAGG CTCTGGTAAA 
GATAAAGAAC AAGATGCAAG TCAAACCACA ACCAAACAAG AACCTGCCAC TGGGAATCAA 
GTCATATTAG CACCCAGTGG AAAAGATGAT GCCGTCTCAT CAAATAAACC CAGAACTGGT 
GGAAAGGAGC GAGGTAACAC TGGGGGTTCA GGCAAAGGAT CCAAAAATGG GGAAAGCTCC 
ATGCAGAATT CTGGTTATTC AGCCTCTCAG TCCAGGAGGG AAAGAGAACA TTCACCTGAA 
AGAGGGAGCA CAACCTACCA TTCGTCCTCA TCCAGAGGTA GCCGATCCAA TCGTGGCAGA 
GGAGAGTTCT ATGGTCGAGG CAGAGGCTAT AGGGGCACTT ACGTGGGTAG TGCAGGTGGT 
GGCAACCGTG GAAAGGCTGG TGGCAGGAAC AGCAGGGATT ATAGGCCAGC TGCCAATAGT 
GGTTACCACC ATCCATCTTC TAATCAAGAT TACAAAAGAG AGGCATCATC TGGGAGTGGC 
AGACATGGCA CATCTCAACC CAATCCTGGA CGTGCTAGGA ACCGTAGTGA GACTCGAAGC 
GAAGGCTCTG AATATGAAGA GGTGCCAAAG AGAAGGAGAC AACGAGGATC TGAAACAGGC 
AGTGAGAGTG CTGGAAGTGA CCTTGCTCAC TCTGACAAGG AAGATCGCAA GCTCAACACC 
AAGGCTGGAA CAGTAGGAGA GAACCCTACA ACTGGCCCTT CATCTTCTGC TTCAGTCAGA 
AACACTCAGA CTAGAGTATT CACACCAAGG GGAGTACCAT CAAGACGTGG TAGAGGTGGA 
GGTGGTGCTG GAGGGGGTCT CCATAGAAGC GGAGGTGGTG CTGGAGTGTC TGGTGGACAA 
AGACCTGGAC CTGGCTCTTC TTCCCATGGC TGGTCGGCAA AGTCTGCAAA TGTGCGAAAG 
CCACAGACAT CCTCACAGCC ACCCCCTGCT AAAGATTTAA GCCGTGGAGT GAATGTTGAC 
AAGAAGAAAC CTGCAGAACA GAATCAGCCT CAAAGTCAAA GTGCAAATCC CACCACAACT 
GTTGCCTCTG TGCCTACAAC TATCCAACAG GGGTCCACTG AGAATGGAAA TGTTGGAGCA 
CCAATGTCTT CAACTAATGC TCCATCAAAT CCCGCTGGTG CACCTACACA GTCTCTTCCT 
CTGCCTGCCC CAGATGGGCG GAACTTCCCT AACAGGGGAT TTGAGCGCCC TCCTCGTCGT 
AGGCGCCATG GCCGATCCCA GCATCAGCAG GACAAGCCTC CAAGATTCCG CAGACTTAAA 
CAAGAGCGAG AGAATGCAGC CCGTATAAAT GGAAGCAGTG GATTAATTGA AGTCATAGCT 
GCACAACAGC AACGCACTGC TCCACTGTTG CAAAATTCCC TGCAACAAGC CAACAGCACT 
CCCAATGTAC CCGATGCAGT AGTTGCAAAT GCAAATCACA GTGTGTCCAC AACTCCTAAC 
AGTAATAATA ATAGCAGCAA CCTTGCAGGT GGAAACCAAA ATTTCAATAG TCACCACCAC 
CATCACTATC AGGGTAACTC CCAGTCACAC CCTCAGCACC ATCACAACCA TAACCCGGCC 
GGGGGCGCCA AATCTCCAGA TGTCTCCAAC CAGAACTCTG ACCAGGCAAA TGAAGAATGG 
GAAACTGCCT CAGAAAGCAG TGATTTCACA GAGTTTCGAG AGAGAGAGGG TGGAGGAGGT 
GGTAAATCCT ATTCCTCGCA TCACCATCAT CACCACCACC ACCACCATCC ACCGGGAAGA 
GGCGGTGGAG GGGGCGGTGG TGGCGACAGA GAGATGACTG CTAAAGAATT AGCCTCTGGG 
AAGAGGAGCT TCTCCAGTCA GCGTCCAGGA ATGGAGCGGC AGAACAGAAG GGTGAATGCT 
GGTGGTGGTG GTGGAGGAAA AGGCCAGAGG GGTCCGCCCG GCACTGGTGG GCCAGTGGGT 
GGAGCTGGCA ATGGCGGGGG TAATCGTGGT GAGAGGCGTG GCAACTGGCC TTCTCCCAAG 
AACAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2a ( XM_005170017.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170074.1 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X1, mRNA.

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CloneID	ODa24123
Clone ID Related Accession (Same CDS sequence)	XM_005170017.4 , XM_005170017.3 , XM_009294206.2 , XM_009294206.3 , NM_001365423.1
Accession Version	XM_005170017.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein PRRC2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170017.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170074.1
CDS	331..6009
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFVSMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	XM_005170017.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170017.4

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2a ( XM_005170017.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170074.1 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X1, mRNA.

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CloneID	ODa24123
Clone ID Related Accession (Same CDS sequence)	XM_005170017.4 , XM_005170017.3 , XM_009294206.2 , XM_009294206.3 , NM_001365423.1
Accession Version	XM_005170017.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170074.1
CDS	321..5999
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFVSMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	XM_005170017.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170017.3

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2a ( XM_009294206.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292481.1 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X2, mRNA.

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CloneID	ODa24123
Clone ID Related Accession (Same CDS sequence)	XM_005170017.4 , XM_005170017.3 , XM_009294206.2 , XM_009294206.3 , NM_001365423.1
Accession Version	XM_009294206.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292481.1
CDS	157..5835
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFVSMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	XM_009294206.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294206.2

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2a ( XM_009294206.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292481.1 Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X2, mRNA.

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CloneID	ODa24123
Clone ID Related Accession (Same CDS sequence)	XM_005170017.4 , XM_005170017.3 , XM_009294206.2 , XM_009294206.3 , NM_001365423.1
Accession Version	XM_009294206.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein PRRC2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292481.1
CDS	174..5852
Translation	MSERSGQTAKGKDGKTKYASLNLFDTYKGKSLEAQKPVVAPRHGLQSLGKVASTRRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEQADPKSTDVLSAAQPESQQPVASQTSAPSIPRTPPAQEPPTPALAVGPRSWAPASVTHGAQGDGGKGSNQPSPFSREEFPTLQAAGDQDRAGKEQATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLAGEAVAEGGAGGVILMEGAAGAPPIQQQNASSHGPPRTPPTSNPALPLPQPTIGPQFQAYRGIMPPFVSMYPPYLPFPPPYGPQGPYRYPPPNEAPRFSRNQGGIGPDGRPPGGPRGEVVKRPSILKQDDLKELDELDHDGDEGWAGAQEEIDYSAKLKFSDDEGEDDGEEERGENKNGTRESREQQRSQDGPVPVSRSRASDSGGDSRRTPPSSTEDGSVSPSSKPGWAEEGGSSWNSQANAGSYQDRPSNQSPIPGPILAPQKPSSAHAQQQAGTVPGGPTPPPSNTLHPQQQGEDEDETWRQRRKQSSTEISAAVERARRRREEEERRMQEERRAACAEKLKRLDEKQQQQTIKPSSPSDAPANSPSPSLSAHASSPNVSQPSSPCVDTEEPPLLSAQGSGATLGLTNNNRQRAGSNSSHDSNNESQQVLQPQATQHPQSQDVPGPGDIKEEHVVGGPHIRSTRVGDDSVKVVECVGVTGRQGSGPSGQGFSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQQQHNQVAAQNQLQSQPQNQQGPAPGTAPQSGPKQPTLYPPGSMGRPPPLPMNFDPRWMMMPYMDPRMMQGRPPPMDYYPPSMHPSGMMNRERSDSGGSGSDPFDRQQHAGPPHSHRGTPPMDPKLAWGPDVFPGGTEGRGLSSPLRQKAVEEEEAGKGQRSDTPPVRMREGGTASVQQPGVAPSSPSGSSNQTPPPLGIQVGGAGHHAHHQPFLDGRGGYSSYPDNGSRMGSHLQQRANSGGTLHGFGHQEGSQPNQIWGTPHPHYDRSDHSALESNSHHQHHGPPSHAPHFSLHPHKQENGREREKMVEPKKNDPSPPLHQPSLSSSCSSSSSSSSAREDGSGKQSLHNQVPPLHEHDTGSTHASGRKQDKTGNAHNQPPQHSGQQHHPRANPRNREHKTETHWGPRPGSSSVSGSSTHNRKTGSGIIAITEPSGNQGAENKSFNQSDGITVKRAGPIKRPVLKEIKREGGEGGGEKTTGGSGKDKEQDASQTTTKQEPATGNQVILAPSGKDDAVSSNKPRTGGKERGNTGGSGKGSKNGESSMQNSGYSASQSRREREHSPERGSTTYHSSSSRGSRSNRGRGEFYGRGRGYRGTYVGSAGGGNRGKAGGRNSRDYRPAANSGYHHPSSNQDYKREASSGSGRHGTSQPNPGRARNRSETRSEGSEYEEVPKRRRQRGSETGSESAGSDLAHSDKEDRKLNTKAGTVGENPTTGPSSSASVRNTQTRVFTPRGVPSRRGRGGGGAGGGLHRSGGGAGVSGGQRPGPGSSSHGWSAKSANVRKPQTSSQPPPAKDLSRGVNVDKKKPAEQNQPQSQSANPTTTVASVPTTIQQGSTENGNVGAPMSSTNAPSNPAGAPTQSLPLPAPDGRNFPNRGFERPPRRRRHGRSQHQQDKPPRFRRLKQERENAARINGSSGLIEVIAAQQQRTAPLLQNSLQQANSTPNVPDAVVANANHSVSTTPNSNNNSSNLAGGNQNFNSHHHHHYQGNSQSHPQHHHNHNPAGGAKSPDVSNQNSDQANEEWETASESSDFTEFREREGGGGGKSYSSHHHHHHHHHHPPGRGGGGGGGGDREMTAKELASGKRSFSSQRPGMERQNRRVNAGGGGGGKGQRGPPGTGGPVGGAGNGGGNRGERRGNWPSPKNRK

Target ORF information:

RefSeq Version	XM_009294206.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2A (prrc2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294206.3

ORF Insert Sequence:

ATGTCAGAGC GCTCTGGGCA AACGGCAAAG GGGAAGGATG GCAAAACCAA GTATGCGTCT 
CTCAACCTGT TTGATACCTA CAAAGGAAAG AGCCTTGAAG CACAGAAGCC AGTTGTTGCC 
CCTCGTCATG GCTTACAGTC TCTTGGTAAA GTTGCCTCTA CACGGCGCAT GCCCCCCCCT 
GCCAACTTGC CTAGTTTGAA GGCAGAGAAC AAAGGCAACG ACCCCAACGT CTCGCTCGTT 
CCCAAAGACG GCACAGGATG GGCAAGCAAG CAGGAACAAG CAGACCCAAA GAGTACCGAT 
GTATTGTCAG CAGCGCAGCC GGAGTCGCAG CAGCCTGTGG CCTCACAGAC GTCTGCGCCG 
AGCATACCGA GAACACCCCC AGCTCAAGAG CCTCCGACTC CAGCTCTAGC CGTAGGGCCA 
AGGTCTTGGG CTCCGGCCAG TGTTACACAT GGAGCACAAG GAGATGGTGG AAAGGGATCA 
AACCAACCAT CGCCATTCTC TCGCGAGGAA TTTCCCACCC TGCAGGCGGC TGGAGACCAG 
GACAGAGCTG GCAAAGAACA GGCCACTGCA GATCAGTGGT ATGGGCCAGG ACCAAGCCTC 
CGCCCCCAGA ACGTAACAAG TTGGCGGGAT GGTGGGGGCC GGGCATTGGC ACCCACCCTG 
GCTGGGGAGG CAGTTGCGGA GGGCGGTGCT GGGGGAGTCA TCCTGATGGA GGGTGCTGCT 
GGGGCCCCTC CCATCCAACA GCAGAATGCA TCTTCTCATG GGCCGCCTAG AACTCCCCCA 
ACCAGCAACC CCGCGCTTCC CCTCCCCCAG CCAACTATTG GCCCGCAGTT TCAGGCTTAC 
AGAGGGATCA TGCCTCCCTT CGTAAGTATG TATCCACCAT ACCTGCCCTT TCCACCCCCT 
TATGGTCCAC AGGGACCTTA CAGGTACCCA CCGCCAAATG AGGCTCCTAG GTTTTCTCGG 
AACCAGGGTG GAATAGGGCC AGATGGGCGA CCCCCTGGAG GCCCCCGGGG TGAAGTGGTA 
AAACGCCCAT CCATCCTCAA ACAGGATGAC CTAAAAGAGC TGGATGAGCT GGACCATGAT 
GGAGATGAGG GCTGGGCTGG AGCTCAAGAG GAAATTGACT ACTCCGCGAA GTTGAAATTC 
AGTGACGATG AAGGAGAGGA TGATGGGGAG GAGGAACGGG GTGAAAATAA AAATGGAACC 
CGGGAGTCAA GGGAACAGCA GAGATCCCAA GATGGACCTG TACCTGTTTC ACGTTCCAGG 
GCATCCGACA GTGGAGGGGA TTCGCGACGC ACCCCTCCTT CCAGTACTGA AGATGGCTCT 
GTGTCCCCCT CCAGCAAGCC AGGCTGGGCC GAGGAGGGAG GTAGCAGCTG GAACAGTCAA 
GCAAATGCCG GATCTTATCA GGACCGGCCT TCAAACCAGT CGCCAATACC TGGCCCAATC 
CTGGCCCCCC AGAAGCCCAG TAGTGCTCAT GCACAGCAGC AGGCAGGGAC AGTGCCTGGT 
GGGCCAACTC CTCCTCCCTC CAACACCCTG CACCCTCAGC AACAGGGTGA GGATGAAGAT 
GAGACATGGC GACAAAGGAG AAAGCAGTCA TCAACCGAGA TATCTGCAGC AGTGGAGCGT 
GCCCGTCGAC GCCGTGAAGA GGAGGAACGG AGGATGCAGG AGGAGCGCCG TGCTGCCTGT 
GCAGAGAAGC TGAAAAGACT TGATGAAAAA CAGCAGCAAC AGACAATAAA ACCCAGCAGC 
CCCAGTGACG CTCCAGCCAA CAGCCCTAGT CCTTCTTTGT CTGCACATGC CTCATCTCCC 
AATGTCAGTC AGCCATCATC ACCCTGCGTG GACACTGAAG AACCACCTCT GTTATCCGCT 
CAAGGAAGTG GTGCAACTCT AGGGCTTACT AATAATAACA GACAGAGAGC TGGTAGCAAC 
AGCAGCCACG ACTCCAACAA TGAGTCCCAG CAGGTTCTGC AACCTCAAGC CACCCAGCAT 
CCACAATCCC AGGATGTCCC AGGGCCTGGA GATATCAAGG AGGAGCATGT GGTGGGCGGT 
CCACACATCC GCAGTACTAG AGTTGGAGAT GATTCAGTGA AGGTGGTTGA ATGTGTTGGT 
GTAACAGGAC GGCAGGGAAG TGGTCCTTCT GGGCAAGGCT TTTCCAAGTA TCAAAAATCG 
CTTCCTCCTC GCTTTCAGAG GCAACAACAG GAGCAACTTC TCAAACAGCA ACAGCAGTGG 
CAGCAGCAAC AACAGCAACA TAACCAGGTT GCTGCCCAAA ATCAGTTGCA GTCACAGCCT 
CAGAACCAGC AGGGCCCCGC TCCAGGTACC GCACCACAGT CTGGGCCCAA ACAGCCAACT 
CTTTATCCTC CTGGCTCAAT GGGACGTCCT CCACCTCTTC CTATGAACTT TGATCCCCGC 
TGGATGATGA TGCCCTATAT GGACCCCCGA ATGATGCAGG GACGCCCCCC TCCTATGGAC 
TACTACCCTC CAAGCATGCA TCCTTCAGGG ATGATGAATC GAGAGCGTTC TGATTCAGGT 
GGCTCTGGGT CAGATCCCTT TGATAGGCAG CAGCATGCTG GACCCCCTCA TTCCCATCGT 
GGTACACCCC CCATGGATCC CAAACTGGCT TGGGGACCAG ATGTTTTTCC AGGAGGAACA 
GAGGGACGAG GTCTAAGCTC CCCTTTACGT CAGAAAGCAG TTGAGGAAGA GGAGGCTGGA 
AAAGGTCAAA GAAGTGACAC TCCTCCCGTT CGTATGCGAG AAGGAGGCAC AGCGTCTGTT 
CAGCAGCCTG GTGTGGCTCC CAGCTCTCCA TCTGGCTCAT CCAACCAAAC CCCACCACCT 
CTGGGGATTC AAGTTGGGGG TGCAGGCCAC CATGCCCATC ATCAACCCTT CTTGGATGGG 
CGAGGAGGTT ACAGCAGCTA CCCTGACAAT GGATCAAGAA TGGGCTCCCA CCTGCAGCAA 
AGGGCTAACA GTGGAGGGAC ACTGCATGGT TTTGGCCATC AGGAAGGTAG TCAGCCGAAC 
CAAATCTGGG GGACTCCTCA TCCTCATTAT GACCGTTCTG ATCACTCAGC CTTGGAGAGC 
AACTCTCATC ACCAACATCA TGGTCCTCCC TCTCACGCCC CTCATTTTTC TCTTCATCCT 
CATAAGCAAG AGAATGGCAG GGAGCGAGAA AAAATGGTGG AGCCAAAGAA GAATGACCCT 
TCACCTCCAC TCCATCAGCC GTCCCTCTCC TCTTCCTGTT CATCATCATC CTCGTCTTCC 
TCTGCCAGAG AAGATGGCAG TGGAAAACAG TCTTTACATA ATCAAGTTCC ACCACTGCAT 
GAGCATGATA CTGGATCCAC TCATGCTTCA GGAAGGAAGC AGGACAAGAC AGGAAATGCG 
CATAATCAGC CCCCACAACA TTCTGGGCAG CAGCACCACC CTAGAGCCAA CCCTCGCAAT 
CGGGAACACA AAACTGAGAC GCACTGGGGC CCGAGGCCTG GTAGCAGTAG TGTGAGTGGA 
AGCTCTACTC ACAACAGAAA GACAGGCTCT GGAATTATTG CAATTACAGA ACCATCTGGA 
AACCAGGGTG CTGAGAACAA GTCCTTTAAC CAATCAGATG GCATCACAGT CAAGCGGGCA 
GGTCCTATTA AGAGACCAGT TCTTAAAGAG ATTAAAAGGG AGGGTGGTGA AGGAGGAGGA 
GAAAAGACTA CTGGAGGCTC TGGTAAAGAT AAAGAACAAG ATGCAAGTCA AACCACAACC 
AAACAAGAAC CTGCCACTGG GAATCAAGTC ATATTAGCAC CCAGTGGAAA AGATGATGCC 
GTCTCATCAA ATAAACCCAG AACTGGTGGA AAGGAGCGAG GTAACACTGG GGGTTCAGGC 
AAAGGATCCA AAAATGGGGA AAGCTCCATG CAGAATTCTG GTTATTCAGC CTCTCAGTCC 
AGGAGGGAAA GAGAACATTC ACCTGAAAGA GGGAGCACAA CCTACCATTC GTCCTCATCC 
AGAGGTAGCC GATCCAATCG TGGCAGAGGA GAGTTCTATG GTCGAGGCAG AGGCTATAGG 
GGCACTTACG TGGGTAGTGC AGGTGGTGGC AACCGTGGAA AGGCTGGTGG CAGGAACAGC 
AGGGATTATA GGCCAGCTGC CAATAGTGGT TACCACCATC CATCTTCTAA TCAAGATTAC 
AAAAGAGAGG CATCATCTGG GAGTGGCAGA CATGGCACAT CTCAACCCAA TCCTGGACGT 
GCTAGGAACC GTAGTGAGAC TCGAAGCGAA GGCTCTGAAT ATGAAGAGGT GCCAAAGAGA 
AGGAGACAAC GAGGATCTGA AACAGGCAGT GAGAGTGCTG GAAGTGACCT TGCTCACTCT 
GACAAGGAAG ATCGCAAGCT CAACACCAAG GCTGGAACAG TAGGAGAGAA CCCTACAACT 
GGCCCTTCAT CTTCTGCTTC AGTCAGAAAC ACTCAGACTA GAGTATTCAC ACCAAGGGGA 
GTACCATCAA GACGTGGTAG AGGTGGAGGT GGTGCTGGAG GGGGTCTCCA TAGAAGCGGA 
GGTGGTGCTG GAGTGTCTGG TGGACAAAGA CCTGGACCTG GCTCTTCTTC CCATGGCTGG 
TCGGCAAAGT CTGCAAATGT GCGAAAGCCA CAGACATCCT CACAGCCACC CCCTGCTAAA 
GATTTAAGCC GTGGAGTGAA TGTTGACAAG AAGAAACCTG CAGAACAGAA TCAGCCTCAA 
AGTCAAAGTG CAAATCCCAC CACAACTGTT GCCTCTGTGC CTACAACTAT CCAACAGGGG 
TCCACTGAGA ATGGAAATGT TGGAGCACCA ATGTCTTCAA CTAATGCTCC ATCAAATCCC 
GCTGGTGCAC CTACACAGTC TCTTCCTCTG CCTGCCCCAG ATGGGCGGAA CTTCCCTAAC 
AGGGGATTTG AGCGCCCTCC TCGTCGTAGG CGCCATGGCC GATCCCAGCA TCAGCAGGAC 
AAGCCTCCAA GATTCCGCAG ACTTAAACAA GAGCGAGAGA ATGCAGCCCG TATAAATGGA 
AGCAGTGGAT TAATTGAAGT CATAGCTGCA CAACAGCAAC GCACTGCTCC ACTGTTGCAA 
AATTCCCTGC AACAAGCCAA CAGCACTCCC AATGTACCCG ATGCAGTAGT TGCAAATGCA 
AATCACAGTG TGTCCACAAC TCCTAACAGT AATAATAATA GCAGCAACCT TGCAGGTGGA 
AACCAAAATT TCAATAGTCA CCACCACCAT CACTATCAGG GTAACTCCCA GTCACACCCT 
CAGCACCATC ACAACCATAA CCCGGCCGGG GGCGCCAAAT CTCCAGATGT CTCCAACCAG 
AACTCTGACC AGGCAAATGA AGAATGGGAA ACTGCCTCAG AAAGCAGTGA TTTCACAGAG 
TTTCGAGAGA GAGAGGGTGG AGGAGGTGGT AAATCCTATT CCTCGCATCA CCATCATCAC 
CACCACCACC ACCATCCACC GGGAAGAGGC GGTGGAGGGG GCGGTGGTGG CGACAGAGAG 
ATGACTGCTA AAGAATTAGC CTCTGGGAAG AGGAGCTTCT CCAGTCAGCG TCCAGGAATG 
GAGCGGCAGA ACAGAAGGGT GAATGCTGGT GGTGGTGGTG GAGGAAAAGG CCAGAGGGGT 
CCGCCCGGCA CTGGTGGGCC AGTGGGTGGA GCTGGCAATG GCGGGGGTAA TCGTGGTGAG 
AGGCGTGGCA ACTGGCCTTC TCCCAAGAAC AGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Presence of two tumor necrosis factor (tnf)-? homologs on different chromosomes of zebrafish (Danio rerio) and medaka (Oryzias latipes).
Marine genomics131-9(2014 Feb)
Kinoshita S,Biswas G,Kono T,Hikima J,Sakai M

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish.
BMC genomics6152(2005 Nov)
Sambrook JG,Figueroa F,Beck S

Conservation of Mhc class III region synteny between zebrafish and human as determined by radiation hybrid mapping.
Journal of immunology (Baltimore, Md. : 1950)165(12)6984-93(2000 Dec)
S?ltmann H,Sato A,Murray BW,Takezaki N,Geisler R,Rauch GJ,Klein J

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prrc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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