panx1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	panx1a
Entrez Gene ID	393890
Full Name	pannexin 1a
Synonyms	panx1,zgc:55631
General protein information	Preferred Names pannexin 1a Names pannexin-1 pannexin 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200916.1	NP_957210.1	pannexin-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PANX1	XP_849329.1
Rattus norvegicus (Norway rat)	Panx1	NP_955429.1
Danio rerio (zebrafish)	panx1a	NP_957210.1
Pan troglodytes (chimpanzee)	PANX1	XP_522150.2
Mus musculus (house mouse)	Panx1	NP_062355.2
Bos taurus (cattle)	PANX1	NP_001232854.1
Xenopus tropicalis (tropical clawed frog)	panx1	NP_001123728.1
Homo sapiens (human)	PANX1	NP_056183.2
	PANX1	XP_001088479.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Extracellular ATP hydrolysis inhibits synaptic transmission by increasing ph buffering in the synaptic cleft.
Vroman R, Klaassen LJ, Howlett MH, Cenedese V, Klooster J, Sjoerdsma T, Kamermans M
PLoS biology12(5)e1001864(2014 May)

Pannexin1 channel proteins in the zebrafish retina have shared and unique properties.
Kurtenbach S, Prochnow N, Kurtenbach S, Klooster J, Zoidl C, Dermietzel R, Kamermans M, Zoidl G
PloS one8(10)e77722(2013)

Reciprocal regulation between resting microglial dynamics and neuronal activity in vivo.
Li Y, Du XF, Liu CS, Wen ZL, Du JL
Developmental cell23(6)1189-202(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Extracellular ATP hydrolysis via NTPDase1 action inhibits synaptic transmission by pannexin 1-mediated increase in pH buffering of the synaptic cleft.
Title: Extracellular ATP hydrolysis inhibits synaptic transmission by increasing ph buffering in the synaptic cleft.

The existence of two Panx1 proteins in zebrafish displaying distinct tissue distribution, protein modification and electrophysiological properties, suggests that both proteins fulfill different functions in vivo.
Title: Pannexin1 channel proteins in the zebrafish retina have shared and unique properties.

The function of panx1 channel is significantly reduced following mutation of a single cysteine residue (C282W) in the fourth transmembrane region of panx1.
Title: Replacement of a single cysteine in the fourth transmembrane region of zebrafish pannexin 1 alters hemichannel gating behavior.

zfPanx1 displays properties similar to its mammalian homologues and can potentially play an important role in functions of the outer retina.
Title: Pannexin1 in the outer retina of the zebrafish, Danio rerio.

The following panx1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the panx1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35073	NM_200916.1 Latest version!	Danio rerio pannexin 1a (panx1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

panx1a ( NM_200916.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957210.1 Danio rerio pannexin 1a (panx1a), mRNA.

Price & Availability↑

CloneID	ODa35073
Clone ID Related Accession (Same CDS sequence)	NM_200916.1
Accession Version	NM_200916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	pannexin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048058.1. On Jul 6, 2005 this sequence version replaced XM_697309.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC048058.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTATAG CGCACGCGGC CACGGAGTAT GTGTTCGCGG ATTTTGTGCT GAAGGACCCG 
GCGTCAGAAA ACAGGTATAA AGGCATTCGC TTAGATTTAG CCCTGGATAA AATAGTCACC 
TGTGTCGCCG TGGGTCTACC TTTGCTTCTC ATTTCTCTAG CCTTCGCTCA GGAAGTTTCT 
GTCGGTACAC AGATCAGTTG TTTCGCCCCC ACAGCGTTCT CATGGTGTCA GGCAGCTTAC 
GTGGATTCAT ACTGTTGGGC AGCAGTGCAG AAACAGGACA CTGGAGGCCT TCCTTTATGG 
CTGCATAAGT TTTTCCCTTA TATCCTGCTG CTGGTGGCAG TGTCAGTTTA CATGCCAGCG 
TTGTTTTGGC GGTTTACAGG AGCCCCGGTG CTTTCTTCTG ACCTCACATT TATAATGGAA 
GAACTGGACC GTTCCTACAA CAGAGCCATC AAATTGGCTA AATGCCTTCA CACTGCAGGA 
AAAGTAGAAA CACCGCCAGC TCCAGTGGAT ACAAACAGCT CTGTGATAGA CCTCACCGAG 
AGCTATTTTA AATATCCTCT GGTGGAGCAG TACTTGAGGA CCAAGCGCTA CTCTAAAGGA 
TTACTGATCC GCTACCTTCT TTGCCGCACC ATCACTGTCT TGGCCCTCAC TCTAGCCTGC 
ATCTACCTTT GCTATTACAT CAGCCTCTCC ACCACCGATG AGTTTTCGTG TGACATCCGC 
TCTGGTGTGG TTGTCAATGA CTCCTCCGTG CCTCCAGCCC TGCAGTGCAA GCTAGTTGCT 
GTGGGCATCT TCCAGCTCCT GAGCTACATC AACCTTTCAG TGTATTTGCT GCTGTTGCCA 
TTGTGCATGT TTGCCATGTT GGTTCCATTC AGAAGGACCA AGGGCTTCCT GAAGCCTTTC 
GAGATGTTGC CCACAATAGG GGTGATGCAG TTTGGGCAGG CGACCTGGGA TGATCTTGCT 
CTGTATCTGC TGTTTCTGGA GGAGAACCTC AGTGAGCTGA AGAGCTATAA GTGCCTTAAG 
GTATTAGAGT TGTTAAAAGA GCAGGGAGAT GAGTCTTTTG ACCCCATGTT GCTGTTGCGG 
ACACTTGGAC AGGTTAAAAC AGATGTGGTA GACGGCAGAT TACCGCAAAA GGACCAGAAA 
ATCTCCAGCA CTAATAATGG GGACACAGAG CTGAAAGAGT TTGCTCCTCT GCTGCCGGAG 
GACTGCTTGC GAAAGCATGA AGACGAGAAG GCTGTCCGCC AGAGGGTCAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957210.1
CDS	254..1507
Translation	MAIAHAATEYVFADFVLKDPASENRYKGIRLDLALDKIVTCVAVGLPLLLISLAFAQEVSVGTQISCFAPTAFSWCQAAYVDSYCWAAVQKQDTGGLPLWLHKFFPYILLLVAVSVYMPALFWRFTGAPVLSSDLTFIMEELDRSYNRAIKLAKCLHTAGKVETPPAPVDTNSSVIDLTESYFKYPLVEQYLRTKRYSKGLLIRYLLCRTITVLALTLACIYLCYYISLSTTDEFSCDIRSGVVVNDSSVPPALQCKLVAVGIFQLLSYINLSVYLLLLPLCMFAMLVPFRRTKGFLKPFEMLPTIGVMQFGQATWDDLALYLLFLEENLSELKSYKCLKVLELLKEQGDESFDPMLLLRTLGQVKTDVVDGRLPQKDQKISSTNNGDTELKEFAPLLPEDCLRKHEDEKAVRQRVI

Target ORF information:

RefSeq Version	NM_200916.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pannexin 1a (panx1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200916.1

ORF Insert Sequence:

ATGGCTATAG CGCACGCGGC CACGGAGTAT GTGTTCGCGG ATTTTGTGCT GAAGGACCCG 
GCGTCAGAAA ACAGGTATAA AGGCATTCGC TTAGATTTAG CCCTGGATAA AATAGTCACC 
TGTGTCGCCG TGGGTCTACC TTTGCTTCTC ATTTCTCTAG CCTTCGCTCA GGAAGTTTCT 
GTCGGTACAC AGATCAGTTG TTTCGCCCCC ACAGCGTTCT CATGGTGTCA GGCAGCTTAC 
GTGGATTCAT ACTGTTGGGC AGCAGTGCAG AAACAGGACA CTGGAGGCCT TCCTTTATGG 
CTGCATAAGT TTTTCCCTTA TATCCTGCTG CTGGTGGCAG TGTCAGTTTA CATGCCAGCG 
TTGTTTTGGC GGTTTACAGG AGCCCCGGTG CTTTCTTCTG ACCTCACATT TATAATGGAA 
GAACTGGACC GTTCCTACAA CAGAGCCATC AAATTGGCTA AATGCCTTCA CACTGCAGGA 
AAAGTAGAAA CACCGCCAGC TCCAGTGGAT ACAAACAGCT CTGTGATAGA CCTCACCGAG 
AGCTATTTTA AATATCCTCT GGTGGAGCAG TACTTGAGGA CCAAGCGCTA CTCTAAAGGA 
TTACTGATCC GCTACCTTCT TTGCCGCACC ATCACTGTCT TGGCCCTCAC TCTAGCCTGC 
ATCTACCTTT GCTATTACAT CAGCCTCTCC ACCACCGATG AGTTTTCGTG TGACATCCGC 
TCTGGTGTGG TTGTCAATGA CTCCTCCGTG CCTCCAGCCC TGCAGTGCAA GCTAGTTGCT 
GTGGGCATCT TCCAGCTCCT GAGCTACATC AACCTTTCAG TGTATTTGCT GCTGTTGCCA 
TTGTGCATGT TTGCCATGTT GGTTCCATTC AGAAGGACCA AGGGCTTCCT GAAGCCTTTC 
GAGATGTTGC CCACAATAGG GGTGATGCAG TTTGGGCAGG CGACCTGGGA TGATCTTGCT 
CTGTATCTGC TGTTTCTGGA GGAGAACCTC AGTGAGCTGA AGAGCTATAA GTGCCTTAAG 
GTATTAGAGT TGTTAAAAGA GCAGGGAGAT GAGTCTTTTG ACCCCATGTT GCTGTTGCGG 
ACACTTGGAC AGGTTAAAAC AGATGTGGTA GACGGCAGAT TACCGCAAAA GGACCAGAAA 
ATCTCCAGCA CTAATAATGG GGACACAGAG CTGAAAGAGT TTGCTCCTCT GCTGCCGGAG 
GACTGCTTGC GAAAGCATGA AGACGAGAAG GCTGTCCGCC AGAGGGTCAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Extracellular ATP hydrolysis inhibits synaptic transmission by increasing ph buffering in the synaptic cleft.
PLoS biology12(5)e1001864(2014 May)
Vroman R,Klaassen LJ,Howlett MH,Cenedese V,Klooster J,Sjoerdsma T,Kamermans M

Pannexin1 channel proteins in the zebrafish retina have shared and unique properties.
PloS one8(10)e77722(2013)
Kurtenbach S,Prochnow N,Kurtenbach S,Klooster J,Zoidl C,Dermietzel R,Kamermans M,Zoidl G

Reciprocal regulation between resting microglial dynamics and neuronal activity in vivo.
Developmental cell23(6)1189-202(2012 Dec)
Li Y,Du XF,Liu CS,Wen ZL,Du JL