flot2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	flot2b
Entrez Gene ID	393612
Full Name	flotillin 2b
Synonyms	zgc:64103
General protein information	Preferred Names flotillin 2b Names flotillin 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200639.1	NP_956933.1	flotillin 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FLOT2	XP_001141137.1
Mus musculus (house mouse)	Flot2	NP_001271156.1
Homo sapiens (human)	FLOT2	NP_004466.2
	FLOT2	XP_001107301.2
Danio rerio (zebrafish)	flot2a	NP_998240.2
Drosophila melanogaster (fruit fly)	Flo-2	NP_001259553.1
Canis lupus familiaris (dog)	FLOT2	XP_003639329.1
Rattus norvegicus (Norway rat)	Flot2	NP_114018.1
Danio rerio (zebrafish)	flot2b	NP_956933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003789	XP_310341.7
Bos taurus (cattle)	FLOT2	NP_001030543.2
Xenopus tropicalis (tropical clawed frog)	flot2	NP_001011034.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

The regulation of transcriptome responses in zebrafish embryo exposure to triadimefon.
Hsu LS, Chiou BH, Hsu TW, Wang CC, Chen SC
Environmental toxicology32(1)217-226(2017 Jan)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolution of the tbx6/16 subfamily genes in vertebrates: insights from zebrafish.
Ahn D, You KH, Kim CH
Molecular biology and evolution29(12)3959-83(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following flot2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the flot2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34590	NM_200639.1 Latest version!	Danio rerio flotillin 2b (flot2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

flot2b ( NM_200639.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956933.1 Danio rerio flotillin 2b (flot2b), mRNA.

Price & Availability↑

CloneID	ODa34590
Clone ID Related Accession (Same CDS sequence)	NM_200639.1
Accession Version	NM_200639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	flotillin 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057431.1. ##Evidence-Data-START## Transcript exon combination :: BC057431.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGTTGCT GCTTGACTGT TGGACCAAAT GAAGCGCTCG TTGTGTCAGG TGCCTGTTGT 
GGGTCAGATG CAAAGACGTA TGTGGTGGGC GGCTGGGCGT GGGCATGGTG GCTTATATCA 
GACACTCAGA GAATTACACT AGAGATTATG ACACTACAGC CAAAATGCGA AGATGTGGAG 
ACAGCGGAGG GAGTCGCCAT TACAGTCACG GGAGTTGCTC AAGTCAAGGT TATGACTGAC 
AAAGACTTGC TGGCTATTGC TTGTGAGCAG TTTCTGGGAA AGTCTGTCAT GGAAATCAAA 
GCTGTGGTTC TGCAAACCCT TGAGGGACAT TTGCGTTCAA TCTTAGGCAC ATTAACCGTG 
GAGCAGATCT ACCAGGACAG AGATCAGTTT GCTCGGCTGG TGAGAGAGGT GGCAGCTCCT 
GACGTGGGCC GCATGGGCAT CGAGATCCTC AGCTTCACTA TCAAAGATGT GTACGATAAG 
CTGGACTACT TGAGTTCTCT TGGAAAGACG CAGACAGCTG CTGTACAAAG AGACGCAGAC 
ATCGGAGTGG CCGAGGCAGA GAGGGATGCT GGGATTAGAG AAGCTGAATG CAAGAAAGAA 
ATGATGGATG TGAAGTTCTT GGCCGACACT AAAATGGCCG ACTCCAAACG AGAGCTTGAG 
CTGCAAAAAG CCGCTTTTAA CCAAGAAGTA AACACTAAGA AAGCCGAGTC TCAGCTGGCC 
TATGAGCTGC AAGCCGCTAA AGAGCAGCAG AAGATCCGAT TGGAGGAGAT CGAAATCGAG 
GTTGTGCAGA GGAAAAAACA AATCTCCATA GAGGAGAGGG AGATTGAGAG GACAGAGAAG 
GAGCTCATCG CTACGGTCAA GCGGCCGGCT GAAGCCGAGG CTTACAAGAT GGAGCAGCTC 
GCTGAAGGAT ACAAAATGCA GAAAGTGCTG ACGGCTCAGG CGGAGGCAGA GAAGATCCGC 
AAGATCGGAG AAGCGGAGGC CATCTCCATC TCATCTGTGG GAAAAGCAGA GGCCGAGAGA 
ATGAGGCTGA AGGCTGAGGC TTATCAGCAG TATGGGGAGG CCGCCAAAAC TGCACTCGTC 
TTGGACGCGC TGCCTAAGAT TGCTGGGAAG GTGTCTGCTC CTCTGGCCAG GACCAATGAG 
ATTGTCATTC TGAGTGGTGA TGGCAGTCGT GTGACCGGGG AAGTGAACCG GCTTCTCGCT 
GAGCTGCCCG TCTCTGTTAA TGCCCTCACC GGTGTGGATC TGTCCAAGAT GCCCCTGCTG 
CAGAAGTTGA CCGGTGCTCA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956933.1
CDS	134..1420
Translation	MGCCLTVGPNEALVVSGACCGSDAKTYVVGGWAWAWWLISDTQRITLEIMTLQPKCEDVETAEGVAITVTGVAQVKVMTDKDLLAIACEQFLGKSVMEIKAVVLQTLEGHLRSILGTLTVEQIYQDRDQFARLVREVAAPDVGRMGIEILSFTIKDVYDKLDYLSSLGKTQTAAVQRDADIGVAEAERDAGIREAECKKEMMDVKFLADTKMADSKRELELQKAAFNQEVNTKKAESQLAYELQAAKEQQKIRLEEIEIEVVQRKKQISIEEREIERTEKELIATVKRPAEAEAYKMEQLAEGYKMQKVLTAQAEAEKIRKIGEAEAISISSVGKAEAERMRLKAEAYQQYGEAAKTALVLDALPKIAGKVSAPLARTNEIVILSGDGSRVTGEVNRLLAELPVSVNALTGVDLSKMPLLQKLTGAQA

Target ORF information:

RefSeq Version	NM_200639.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio flotillin 2b (flot2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200639.1

ORF Insert Sequence:

ATGGGTTGCT GCTTGACTGT TGGACCAAAT GAAGCGCTCG TTGTGTCAGG TGCCTGTTGT 
GGGTCAGATG CAAAGACGTA TGTGGTGGGC GGCTGGGCGT GGGCATGGTG GCTTATATCA 
GACACTCAGA GAATTACACT AGAGATTATG ACACTACAGC CAAAATGCGA AGATGTGGAG 
ACAGCGGAGG GAGTCGCCAT TACAGTCACG GGAGTTGCTC AAGTCAAGGT TATGACTGAC 
AAAGACTTGC TGGCTATTGC TTGTGAGCAG TTTCTGGGAA AGTCTGTCAT GGAAATCAAA 
GCTGTGGTTC TGCAAACCCT TGAGGGACAT TTGCGTTCAA TCTTAGGCAC ATTAACCGTG 
GAGCAGATCT ACCAGGACAG AGATCAGTTT GCTCGGCTGG TGAGAGAGGT GGCAGCTCCT 
GACGTGGGCC GCATGGGCAT CGAGATCCTC AGCTTCACTA TCAAAGATGT GTACGATAAG 
CTGGACTACT TGAGTTCTCT TGGAAAGACG CAGACAGCTG CTGTACAAAG AGACGCAGAC 
ATCGGAGTGG CCGAGGCAGA GAGGGATGCT GGGATTAGAG AAGCTGAATG CAAGAAAGAA 
ATGATGGATG TGAAGTTCTT GGCCGACACT AAAATGGCCG ACTCCAAACG AGAGCTTGAG 
CTGCAAAAAG CCGCTTTTAA CCAAGAAGTA AACACTAAGA AAGCCGAGTC TCAGCTGGCC 
TATGAGCTGC AAGCCGCTAA AGAGCAGCAG AAGATCCGAT TGGAGGAGAT CGAAATCGAG 
GTTGTGCAGA GGAAAAAACA AATCTCCATA GAGGAGAGGG AGATTGAGAG GACAGAGAAG 
GAGCTCATCG CTACGGTCAA GCGGCCGGCT GAAGCCGAGG CTTACAAGAT GGAGCAGCTC 
GCTGAAGGAT ACAAAATGCA GAAAGTGCTG ACGGCTCAGG CGGAGGCAGA GAAGATCCGC 
AAGATCGGAG AAGCGGAGGC CATCTCCATC TCATCTGTGG GAAAAGCAGA GGCCGAGAGA 
ATGAGGCTGA AGGCTGAGGC TTATCAGCAG TATGGGGAGG CCGCCAAAAC TGCACTCGTC 
TTGGACGCGC TGCCTAAGAT TGCTGGGAAG GTGTCTGCTC CTCTGGCCAG GACCAATGAG 
ATTGTCATTC TGAGTGGTGA TGGCAGTCGT GTGACCGGGG AAGTGAACCG GCTTCTCGCT 
GAGCTGCCCG TCTCTGTTAA TGCCCTCACC GGTGTGGATC TGTCCAAGAT GCCCCTGCTG 
CAGAAGTTGA CCGGTGCTCA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

The regulation of transcriptome responses in zebrafish embryo exposure to triadimefon.
Environmental toxicology32(1)217-226(2017 Jan)
Hsu LS,Chiou BH,Hsu TW,Wang CC,Chen SC

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolution of the tbx6/16 subfamily genes in vertebrates: insights from zebrafish.
Molecular biology and evolution29(12)3959-83(2012 Dec)
Ahn D,You KH,Kim CH