get1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	get1
Entrez Gene ID	335756
Full Name	tryptophan rich basic protein
Synonyms	pwi,wu:ft06h05,zgc:101856
General protein information	Preferred Names tryptophan rich basic protein Names tail-anchored protein insertion receptor WRB pinball wizard tryptophan-rich protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003413.1	NP_001003413.1	tail-anchored protein insertion receptor WRB precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wrb	NP_997184.1
Gallus gallus (chicken)	WRB	NP_001072965.1
Pan troglodytes (chimpanzee)	WRB	NP_001238848.1
Rattus norvegicus (Norway rat)	Wrb	NP_955405.1
Homo sapiens (human)	WRB	NP_004618.2
Bos taurus (cattle)	WRB	NP_001029531.1
Danio rerio (zebrafish)	wrb	NP_001003413.1
Xenopus tropicalis (tropical clawed frog)	wrb	NP_001006892.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing.
Vogl C, Panou I, Yamanbaeva G, Wichmann C, Mangosing SJ, Vilardi F, Indzhykulian AA, Pangr?i? T, Santarelli R, Rodriguez-Ballesteros M, Weber T, Jung S, Cardenas E, Wu X, Wojcik SM, Kwan KY, Del Castillo I, Schwappach B, Strenzke N, Corey DP, Lin SY, Moser T
The EMBO journal35(23)2536-2552(2016 12)

Mutation of wrb, a Component of the Guided Entry of Tail-Anchored Protein Pathway, Disrupts Photoreceptor Synapse Structure and Function.
Daniele LL, Emran F, Lobo GP, Gaivin RJ, Perkins BD
Investigative ophthalmology & visual science57(7)2942-54(2016 06)

The zebrafish pinball wizard gene encodes WRB, a tail-anchored-protein receptor essential for inner-ear hair cells and retinal photoreceptors.
Lin SY, Vollrath MA, Mangosing S, Shen J, Cardenas E, Corey DP
The Journal of physiology594(4)895-914(2016 Feb)

Drug-sensitized zebrafish screen identifies multiple genes, including GINS3, as regulators of myocardial repolarization.
Milan DJ, Kim AM, Winterfield JR, Jones IL, Pfeufer A, Sanna S, Arking DE, Amsterdam AH, Sabeh KM, Mably JD, Rosenbaum DS, Peterson RT, Chakravarti A, K??b S, Roden DM, MacRae CA
Circulation120(7)553-9(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wrb may have tissue-specific functions in the visual system and heart, or can maintain dual functions even in the same tissue. The selectivity of the defects at sensory neuron ribbon synapses suggests unique biosynthetic demands for cells bearing ribbon synapses, but may also point to a novel role for Wrb acting through the GET pathway in synaptic function.
Title: Mutation of wrb, a Component of the Guided Entry of Tail-Anchored Protein Pathway, Disrupts Photoreceptor Synapse Structure and Function.

WRB plays a critical role in synaptic functions in inner-ear hair cells and retinal photoreceptors.
Title: The zebrafish pinball wizard gene encodes WRB, a tail-anchored-protein receptor essential for inner-ear hair cells and retinal photoreceptors.

The following get1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the get1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37518	NM_001003413.1 Latest version!	Danio rerio guided entry of tail-anchored proteins factor 1 (get1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

get1 ( NM_001003413.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003413.1 Danio rerio guided entry of tail-anchored proteins factor 1 (get1), mRNA.

Price & Availability↑

CloneID	ODa37518
Clone ID Related Accession (Same CDS sequence)	NM_001003413.1
Accession Version	NM_001003413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Danio rerio(zebrafish)
Product	tail-anchored protein insertion receptor WRB precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY648739.1. ##Evidence-Data-START## Transcript exon combination :: AY648739.1, BC085456.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCG GGTTTAACTG GTTTCTTGTA CTGAGCTCTG TGTTTCTGTG CAACCTCGTC 
AAAACATTCC TGCCATCCAT TTCTTCATTT TTGTCAAAGA TCTTCCATAA AGATGCAGAT 
CAGGAGATGG AGATGAGGAC TGAGATCCAG AACATGAAGA TGGAACTGTC AACCATCAGC 
ATGATGGACG AGTTTGCCAG ATATGCTAGA CTGGAACGCA AGATCAACAA GATGACTGAT 
CAACTCAAAA CTCTTGTTAA ATCCAGAACA GCACAGCAAG CAAAAATGAA GTGGATTGTA 
AATATAGCAT TTTACATTCT TCAGGCAGCC CTCATGATCT CGCTCATACT GAAGTATTAT 
GCAGACCCTG TCACAGTAGT GCCCAGTAAA TGGATCGCTC CATTAGAGAG ACTGGTCGCC 
TTTCCTTCTG GAGTTGCAGG TGGTGTTGGA ATCACATGCT GGCTTGTTGT ATGTAACAAA 
GTGGTGGCCC TCATTCTACA AGCTGTCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003413.1
CDS	13..525
Translation	MAAGFNWFLVLSSVFLCNLVKTFLPSISSFLSKIFHKDADQEMEMRTEIQNMKMELSTISMMDEFARYARLERKINKMTDQLKTLVKSRTAQQAKMKWIVNIAFYILQAALMISLILKYYADPVTVVPSKWIAPLERLVAFPSGVAGGVGITCWLVVCNKVVALILQAVS

Target ORF information:

RefSeq Version	NM_001003413.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guided entry of tail-anchored proteins factor 1 (get1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003413.1

ORF Insert Sequence:

ATGGCTGCCG GGTTTAACTG GTTTCTTGTA CTGAGCTCTG TGTTTCTGTG CAACCTCGTC 
AAAACATTCC TGCCATCCAT TTCTTCATTT TTGTCAAAGA TCTTCCATAA AGATGCAGAT 
CAGGAGATGG AGATGAGGAC TGAGATCCAG AACATGAAGA TGGAACTGTC AACCATCAGC 
ATGATGGACG AGTTTGCCAG ATATGCTAGA CTGGAACGCA AGATCAACAA GATGACTGAT 
CAACTCAAAA CTCTTGTTAA ATCCAGAACA GCACAGCAAG CAAAAATGAA GTGGATTGTA 
AATATAGCAT TTTACATTCT TCAGGCAGCC CTCATGATCT CGCTCATACT GAAGTATTAT 
GCAGACCCTG TCACAGTAGT GCCCAGTAAA TGGATCGCTC CATTAGAGAG ACTGGTCGCC 
TTTCCTTCTG GAGTTGCAGG TGGTGTTGGA ATCACATGCT GGCTTGTTGT ATGTAACAAA 
GTGGTGGCCC TCATTCTACA AGCTGTCAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing.
The EMBO journal35(23)2536-2552(2016 12)
Vogl C,Panou I,Yamanbaeva G,Wichmann C,Mangosing SJ,Vilardi F,Indzhykulian AA,Pangr?i? T,Santarelli R,Rodriguez-Ballesteros M,Weber T,Jung S,Cardenas E,Wu X,Wojcik SM,Kwan KY,Del Castillo I,Schwappach B,Strenzke N,Corey DP,Lin SY,Moser T

Mutation of wrb, a Component of the Guided Entry of Tail-Anchored Protein Pathway, Disrupts Photoreceptor Synapse Structure and Function.
Investigative ophthalmology & visual science57(7)2942-54(2016 06)
Daniele LL,Emran F,Lobo GP,Gaivin RJ,Perkins BD

The zebrafish pinball wizard gene encodes WRB, a tail-anchored-protein receptor essential for inner-ear hair cells and retinal photoreceptors.
The Journal of physiology594(4)895-914(2016 Feb)
Lin SY,Vollrath MA,Mangosing S,Shen J,Cardenas E,Corey DP

Drug-sensitized zebrafish screen identifies multiple genes, including GINS3, as regulators of myocardial repolarization.
Circulation120(7)553-9(2009 Aug)
Milan DJ,Kim AM,Winterfield JR,Jones IL,Pfeufer A,Sanna S,Arking DE,Amsterdam AH,Sabeh KM,Mably JD,Rosenbaum DS,Peterson RT,Chakravarti A,K??b S,Roden DM,MacRae CA

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get1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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