Get1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Get1
Entrez Gene ID	71446
Full Name	tryptophan rich basic protein
Synonyms	5530402J05Rik,C030018G21Rik,Chd5
General protein information	Preferred Names tryptophan rich basic protein Names tail-anchored protein insertion receptor WRB tryptophan-rich protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_207301.2	NP_997184.1	tail-anchored protein insertion receptor WRB
XM_006523090.3	XP_006523153.1	tail-anchored protein insertion receptor WRB isoform X1
XM_006523091.3	XP_006523154.1	tail-anchored protein insertion receptor WRB isoform X2
NM_207301.3	NP_997184.1	tail-anchored protein insertion receptor WRB

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wrb	NP_997184.1
Gallus gallus (chicken)	WRB	NP_001072965.1
Pan troglodytes (chimpanzee)	WRB	NP_001238848.1
Rattus norvegicus (Norway rat)	Wrb	NP_955405.1
Homo sapiens (human)	WRB	NP_004618.2
Bos taurus (cattle)	WRB	NP_001029531.1
Danio rerio (zebrafish)	wrb	NP_001003413.1
Xenopus tropicalis (tropical clawed frog)	wrb	NP_001006892.1

Gene Ontology
Bibliography

Related articles in PubMed

Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing.
Vogl C, Panou I, Yamanbaeva G, Wichmann C, Mangosing SJ, Vilardi F, Indzhykulian AA, Pangr?i? T, Santarelli R, Rodriguez-Ballesteros M, Weber T, Jung S, Cardenas E, Wu X, Wojcik SM, Kwan KY, Del Castillo I, Schwappach B, Strenzke N, Corey DP, Lin SY, Moser T
The EMBO journal35(23)2536-2552(2016 12)

Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo.
Rivera-Monroy J, Musiol L, Unthan-Fechner K, Farkas ?, Clancy A, Coy-Vergara J, Weill U, Gockel S, Lin SY, Corey DP, Kohl T, Str?bel P, Schuldiner M, Schwappach B, Vilardi F
Scientific reports639464(2016 Dec)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

A gene expression map of human chromosome 21 orthologues in the mouse.
Gitton Y, Dahmane N, Baik S, Ruiz i Altaba A, Neidhardt L, Scholze M, Herrmann BG, Kahlem P, Benkahla A, Schrinner S, Yildirimman R, Herwig R, Lehrach H, Yaspo ML,
Nature420(6915)586-90(2002 Dec)

Human chromosome 21 gene expression atlas in the mouse.
Reymond A, Marigo V, Yaylaoglu MB, Leoni A, Ucla C, Scamuffa N, Caccioppoli C, Dermitzakis ET, Lyle R, Banfi S, Eichele G, Antonarakis SE, Ballabio A
Nature420(6915)582-6(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Get1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Get1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08106	NM_207301.3 Latest version!	Mus musculus guided entry of tail-anchored proteins factor 1 (Get1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu52798	XM_006523090.3 Latest version!	Mus musculus tryptophan rich basic protein (Wrb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu52799	XM_006523091.3 Latest version!	Mus musculus tryptophan rich basic protein (Wrb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu08106	NM_207301.2	Mus musculus tryptophan rich basic protein (Wrb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Get1 ( NM_207301.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997184.1 Mus musculus tryptophan rich basic protein (Wrb), mRNA.

Price & Availability↑

CloneID	OMu08106
Clone ID Related Accession (Same CDS sequence)	NM_207301.2 , NM_207301.3
Accession Version	NM_207301.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-27
Organism	Mus musculus(house mouse)
Product	tail-anchored protein insertion receptor WRB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC164647.3. On Nov 17, 2006 this sequence version replaced NM_207301.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC031769.1, AK172079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAGCAG CGCTGATGAT CTCGCTCATT 
TGGAAGTATT ACTCCGTCCC TGTGGCTGTG GTGCCCAGCA AGTGGATCAC CCCACTAGAC 
CGCCTGGTAG CCTTTCCTAC TCGAGTAGCA GGTGGAATTG GAATCACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTGGC TATCGTTCTC CACCCTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997184.1
CDS	59..583
Translation	MSASETDRWAWLLVLSFVFGCNLLRILLPSLSSFISRVLQKDAEQESQMRAEIQGMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWFISVAFYILQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGIGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_207301.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophan rich basic protein (Wrb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207301.2

ORF Insert Sequence:

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAGCAG CGCTGATGAT CTCGCTCATT 
TGGAAGTATT ACTCCGTCCC TGTGGCTGTG GTGCCCAGCA AGTGGATCAC CCCACTAGAC 
CGCCTGGTAG CCTTTCCTAC TCGAGTAGCA GGTGGAATTG GAATCACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTGGC TATCGTTCTC CACCCTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Get1 ( NM_207301.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997184.1 Mus musculus guided entry of tail-anchored proteins factor 1 (Get1), mRNA.

Price & Availability↑

CloneID	OMu08106
Clone ID Related Accession (Same CDS sequence)	NM_207301.2 , NM_207301.3
Accession Version	NM_207301.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	tail-anchored protein insertion receptor WRB
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164647.3. On Apr 20, 2018 this sequence version replaced NM_207301.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC031769.1, AK172079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAGCAG CGCTGATGAT CTCGCTCATT 
TGGAAGTATT ACTCCGTCCC TGTGGCTGTG GTGCCCAGCA AGTGGATCAC CCCACTAGAC 
CGCCTGGTAG CCTTTCCTAC TCGAGTAGCA GGTGGAATTG GAATCACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTGGC TATCGTTCTC CACCCTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997184.1
CDS	78..602
Translation	MSASETDRWAWLLVLSFVFGCNLLRILLPSLSSFISRVLQKDAEQESQMRAEIQGMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWFISVAFYILQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGIGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_207301.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus guided entry of tail-anchored proteins factor 1 (Get1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207301.3

ORF Insert Sequence:

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAGCAG CGCTGATGAT CTCGCTCATT 
TGGAAGTATT ACTCCGTCCC TGTGGCTGTG GTGCCCAGCA AGTGGATCAC CCCACTAGAC 
CGCCTGGTAG CCTTTCCTAC TCGAGTAGCA GGTGGAATTG GAATCACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTGGC TATCGTTCTC CACCCTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Get1 ( XM_006523090.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523153.1 Mus musculus tryptophan rich basic protein (Wrb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52798
Clone ID Related Accession (Same CDS sequence)	XM_006523090.3
Accession Version	XM_006523090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tail-anchored protein insertion receptor WRB isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAAGAC TCATCCACAA AGCCTCGGGC 
ATCCATCCTG CCCTCCTCAG TCCGAGCGTT TCAGCACAGC CTTCAGGACC ACAGAAGGCA 
GCGCTGATGA TCTCGCTCAT TTGGAAGTAT TACTCCGTCC CTGTGGCTGT GGTGCCCAGC 
AAGTGGATCA CCCCACTAGA CCGCCTGGTA GCCTTTCCTA CTCGAGTAGC AGGTGGAATT 
GGAATCACCT GTTGGATTTT AGTCTGTAAC AAAGTTGTGG CTATCGTTCT CCACCCTTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523153.1
CDS	123..728
Translation	MSASETDRWAWLLVLSFVFGCNLLRILLPSLSSFISRVLQKDAEQESQMRAEIQGMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWFISVAFYILQRLIHKASGIHPALLSPSVSAQPSGPQKAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGIGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	XM_006523090.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophan rich basic protein (Wrb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523090.3

ORF Insert Sequence:

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAAGAC TCATCCACAA AGCCTCGGGC 
ATCCATCCTG CCCTCCTCAG TCCGAGCGTT TCAGCACAGC CTTCAGGACC ACAGAAGGCA 
GCGCTGATGA TCTCGCTCAT TTGGAAGTAT TACTCCGTCC CTGTGGCTGT GGTGCCCAGC 
AAGTGGATCA CCCCACTAGA CCGCCTGGTA GCCTTTCCTA CTCGAGTAGC AGGTGGAATT 
GGAATCACCT GTTGGATTTT AGTCTGTAAC AAAGTTGTGG CTATCGTTCT CCACCCTTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Get1 ( XM_006523091.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523154.1 Mus musculus tryptophan rich basic protein (Wrb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu52799
Clone ID Related Accession (Same CDS sequence)	XM_006523091.3
Accession Version	XM_006523091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tail-anchored protein insertion receptor WRB isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAAGAC TCATCCACAA AGCCTCGGGC 
ATCCATCCTG CCCTCCTCAG TCCGAGCGTT TCAGCACAGC CTTCAGGACC ACAGAAGGTG 
CTTCTGGGAA AGGAGAAGAG GCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523154.1
CDS	120..569
Translation	MSASETDRWAWLLVLSFVFGCNLLRILLPSLSSFISRVLQKDAEQESQMRAEIQGMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWFISVAFYILQRLIHKASGIHPALLSPSVSAQPSGPQKVLLGKEKRQR

Target ORF information:

RefSeq Version	XM_006523091.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophan rich basic protein (Wrb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523091.3

ORF Insert Sequence:

ATGAGCGCGT CCGAGACGGA CCGCTGGGCG TGGCTGCTGG TGTTGAGCTT CGTGTTCGGG 
TGTAACTTGC TGCGCATCCT CCTGCCGTCC CTGTCCTCGT TTATTTCCAG GGTGCTGCAG 
AAGGATGCAG AGCAGGAGTC ACAGATGAGG GCAGAGATCC AGGGCATGAA GCAGGAGCTG 
TCCACCGTCA ACATGATGGA CGAGTTTGCC AGATACGCCA GGCTGGAAAG GAAGATCAAC 
AAGATGACGG ACAAGCTCAA AACGCACGTG AAGGCACGGA CAGCTCAGTT GGCCAAGATC 
AAGTGGTTTA TAAGTGTTGC TTTCTACATA CTACAAAGAC TCATCCACAA AGCCTCGGGC 
ATCCATCCTG CCCTCCTCAG TCCGAGCGTT TCAGCACAGC CTTCAGGACC ACAGAAGGTG 
CTTCTGGGAA AGGAGAAGAG GCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing.
The EMBO journal35(23)2536-2552(2016 12)
Vogl C,Panou I,Yamanbaeva G,Wichmann C,Mangosing SJ,Vilardi F,Indzhykulian AA,Pangr?i? T,Santarelli R,Rodriguez-Ballesteros M,Weber T,Jung S,Cardenas E,Wu X,Wojcik SM,Kwan KY,Del Castillo I,Schwappach B,Strenzke N,Corey DP,Lin SY,Moser T

Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo.
Scientific reports639464(2016 Dec)
Rivera-Monroy J,Musiol L,Unthan-Fechner K,Farkas ?,Clancy A,Coy-Vergara J,Weill U,Gockel S,Lin SY,Corey DP,Kohl T,Str?bel P,Schuldiner M,Schwappach B,Vilardi F

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

A gene expression map of human chromosome 21 orthologues in the mouse.
Nature420(6915)586-90(2002 Dec)
Gitton Y,Dahmane N,Baik S,Ruiz i Altaba A,Neidhardt L,Scholze M,Herrmann BG,Kahlem P,Benkahla A,Schrinner S,Yildirimman R,Herwig R,Lehrach H,Yaspo ML,

Human chromosome 21 gene expression atlas in the mouse.
Nature420(6915)582-6(2002 Dec)
Reymond A,Marigo V,Yaylaoglu MB,Leoni A,Ucla C,Scamuffa N,Caccioppoli C,Dermitzakis ET,Lyle R,Banfi S,Eichele G,Antonarakis SE,Ballabio A

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Get1 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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