GET1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GET1
Entrez Gene ID	7485
Full Name	tryptophan rich basic protein
Synonyms	CHD5,GET1
General protein information	Preferred Names tryptophan rich basic protein Names tail-anchored protein insertion receptor WRB congenital heart disease 5 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.2
Summary	This gene is located in the candidate region for congenital heart disease (CHD) in Down syndrome (DS). It encodes a basic protein that functions as a receptor that promotes insertion of tail-anchored proteins in the endoplasmic reticulum membrane. This gene is located at a maternally-methylated differentially methylated region (DMR); however, its transcription may be biallelic, not imprinted. Alternative splicing results in different transcript variants. A pseudogene has been defined on chromosome 4. [provided by RefSeq, Apr 2017].
Disorder MIM:	602915

mRNA and Protein(s)

mRNA	Protein	Name
NM_004627.5	NP_004618.2	tail-anchored protein insertion receptor WRB isoform 1
NM_004627.6	NP_004618.2	tail-anchored protein insertion receptor WRB isoform 1
NM_001350294.1	NP_001337223.1	tail-anchored protein insertion receptor WRB isoform 2
NM_001350296.1	NP_001337225.1	tail-anchored protein insertion receptor WRB isoform 2
NM_001350295.1	NP_001337224.1	tail-anchored protein insertion receptor WRB isoform 2
NM_001350293.1	NP_001337222.1	tail-anchored protein insertion receptor WRB isoform 3
NM_001146218.2	NP_001139690.1	tail-anchored protein insertion receptor WRB isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wrb	NP_997184.1
Gallus gallus (chicken)	WRB	NP_001072965.1
Pan troglodytes (chimpanzee)	WRB	NP_001238848.1
Rattus norvegicus (Norway rat)	Wrb	NP_955405.1
Homo sapiens (human)	WRB	NP_004618.2
Bos taurus (cattle)	WRB	NP_001029531.1
Danio rerio (zebrafish)	wrb	NP_001003413.1
Xenopus tropicalis (tropical clawed frog)	wrb	NP_001006892.1

Gene Ontology
Bibliography

Related articles in PubMed

Trisomy 21 Alters DNA Methylation in Parent-of-Origin-Dependent and -Independent Manners.
Alves da Silva AF, Machado FB, Pavarino ?C, Biselli-P?rico JM, Zampieri BL, da Silva Francisco Junior R, Mozer Rodrigues PT, Terra Machado D, Santos-Rebou?as CB, Gomes Fernandes M, Chuva de Sousa Lopes SM, Lopes Rios ?F, Medina-Acosta E
PloS one11(4)e0154108(2016)

Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.
Yamamoto Y, Sakisaka T
Molecular cell48(3)387-97(2012 Nov)

WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.
Vilardi F, Lorenz H, Dobberstein B
Journal of cell science124(Pt 8)1301-7(2011 Apr)

Identification of a transcriptionally active peroxisome proliferator-activated receptor alpha -interacting cofactor complex in rat liver and characterization of PRIC285 as a coactivator.
Surapureddi S, Yu S, Bu H, Hashimoto T, Yeldandi AV, Kashireddy P, Cherkaoui-Malki M, Qi C, Zhu YJ, Rao MS, Reddy JK
Proceedings of the National Academy of Sciences of the United States of America99(18)11836-41(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WRB gene contains a maternally imprinted differentially methylated region (DMR). The maternally inherited 5mCpG imprints at the WRB DMR are uncoupled from the parental allele expression of WRB and ten neighboring genes in twelve biosamples (brain, blood, fallopian tube, fetal large and small intestine, hESCs, large airway epithelial cells, thyroid, muscle, epidermal keratinocytes, ovary, skin, and testis).
Title: Trisomy 21 Alters DNA Methylation in Parent-of-Origin-Dependent and -Independent Manners.

Results indicate calcium-modulating cyclophilin ligand (CAML) and WRB as components of the TRC40 receptor complex and a crucial mechanism for driving ER membrane insertion of TA proteins in mammalian cells.
Title: Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.

The coiled-coil domain of WRB is the binding site for TRC40/Asna1.
Title: WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.

Observational study of gene-disease association. (HuGE Navigator)
Title: Replication of previous genome-wide association studies of bone mineral density in premenopausal American women.

The following GET1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GET1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01973	NM_004627.6 Latest version!	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01966	NM_001350294.1 Latest version!	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu01966	NM_001350296.1 Latest version!	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu01966	NM_001146218.2 Latest version!	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu01966	NM_001350295.1 Latest version!	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu101318	NM_001350293.1 Latest version!	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu01973	NM_004627.5	Homo sapiens tryptophan rich basic protein (WRB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GET1 ( NM_004627.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004618.2 Homo sapiens tryptophan rich basic protein (WRB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01973
Clone ID Related Accession (Same CDS sequence)	NM_004627.5 , NM_004627.6
Accession Version	NM_004627.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	tail-anchored protein insertion receptor WRB isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF064861.5 and AW001614.1. On Apr 13, 2017 this sequence version replaced NM_004627.4. Transcript Variant: This variant (1) encodes the longest isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: ERR279840.8817.1, SRR1803616.216906.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCAG CCGCGGCCGA CCACTGGGCG TGGTTGCTGG TGCTCAGCTT CGTGTTTGGA 
TGCAATGTTC TTAGGATCCT CCTCCCGTCC TTCTCATCCT TCATGTCCAG GGTGCTGCAG 
AAGGACGCGG AGCAGGAGTC ACAGATGAGA GCGGAGATCC AGGACATGAA GCAGGAGCTC 
TCCACAGTCA ACATGATGGA CGAGTTTGCC AGATATGCCA GGCTGGAAAG AAAGATCAAC 
AAGATGACGG ATAAGCTCAA AACCCATGTG AAAGCTCGGA CAGCTCAATT AGCCAAGATA 
AAATGGGTGA TAAGTGTCGC TTTCTACGTA TTGCAGGCTG CCCTGATGAT CTCACTCATT 
TGGAAGTATT ATTCTGTCCC TGTGGCTGTC GTGCCGAGTA AATGGATAAC CCCTCTAGAC 
CGCCTGGTAG CCTTTCCTAC TAGAGTAGCA GGTGGTGTTG GAATTACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTCGC TATTGTGCTT CATCCGTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004618.2
CDS	142..666
Translation	MSSAAADHWAWLLVLSFVFGCNVLRILLPSFSSFMSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGVGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_004627.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tryptophan rich basic protein (WRB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004627.5

ORF Insert Sequence:

ATGAGCTCAG CCGCGGCCGA CCACTGGGCG TGGTTGCTGG TGCTCAGCTT CGTGTTTGGA 
TGCAATGTTC TTAGGATCCT CCTCCCGTCC TTCTCATCCT TCATGTCCAG GGTGCTGCAG 
AAGGACGCGG AGCAGGAGTC ACAGATGAGA GCGGAGATCC AGGACATGAA GCAGGAGCTC 
TCCACAGTCA ACATGATGGA CGAGTTTGCC AGATATGCCA GGCTGGAAAG AAAGATCAAC 
AAGATGACGG ATAAGCTCAA AACCCATGTG AAAGCTCGGA CAGCTCAATT AGCCAAGATA 
AAATGGGTGA TAAGTGTCGC TTTCTACGTA TTGCAGGCTG CCCTGATGAT CTCACTCATT 
TGGAAGTATT ATTCTGTCCC TGTGGCTGTC GTGCCGAGTA AATGGATAAC CCCTCTAGAC 
CGCCTGGTAG CCTTTCCTAC TAGAGTAGCA GGTGGTGTTG GAATTACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTCGC TATTGTGCTT CATCCGTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GET1 ( NM_004627.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_004618.2 Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01973
Clone ID Related Accession (Same CDS sequence)	NM_004627.5 , NM_004627.6
Accession Version	NM_004627.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-25
Organism	Homo sapiens(human)
Product	tail-anchored protein insertion receptor WRB isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF064861.5 and AW001614.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004627.5. Transcript Variant: This variant (1) encodes the longest isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.257273.1, SRR5189658.143098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000649170.1/ ENSP00000496813.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCAG CCGCGGCCGA CCACTGGGCG TGGTTGCTGG TGCTCAGCTT CGTGTTTGGA 
TGCAATGTTC TTAGGATCCT CCTCCCGTCC TTCTCATCCT TCATGTCCAG GGTGCTGCAG 
AAGGACGCGG AGCAGGAGTC ACAGATGAGA GCGGAGATCC AGGACATGAA GCAGGAGCTC 
TCCACAGTCA ACATGATGGA CGAGTTTGCC AGATATGCCA GGCTGGAAAG AAAGATCAAC 
AAGATGACGG ATAAGCTCAA AACCCATGTG AAAGCTCGGA CAGCTCAATT AGCCAAGATA 
AAATGGGTGA TAAGTGTCGC TTTCTACGTA TTGCAGGCTG CCCTGATGAT CTCACTCATT 
TGGAAGTATT ATTCTGTCCC TGTGGCTGTC GTGCCGAGTA AATGGATAAC CCCTCTAGAC 
CGCCTGGTAG CCTTTCCTAC TAGAGTAGCA GGTGGTGTTG GAATTACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTCGC TATTGTGCTT CATCCGTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004618.2
CDS	60..584
Translation	MSSAAADHWAWLLVLSFVFGCNVLRILLPSFSSFMSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGVGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_004627.6
Organism	Homo sapiens(human)
Definition	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004627.6

ORF Insert Sequence:

ATGAGCTCAG CCGCGGCCGA CCACTGGGCG TGGTTGCTGG TGCTCAGCTT CGTGTTTGGA 
TGCAATGTTC TTAGGATCCT CCTCCCGTCC TTCTCATCCT TCATGTCCAG GGTGCTGCAG 
AAGGACGCGG AGCAGGAGTC ACAGATGAGA GCGGAGATCC AGGACATGAA GCAGGAGCTC 
TCCACAGTCA ACATGATGGA CGAGTTTGCC AGATATGCCA GGCTGGAAAG AAAGATCAAC 
AAGATGACGG ATAAGCTCAA AACCCATGTG AAAGCTCGGA CAGCTCAATT AGCCAAGATA 
AAATGGGTGA TAAGTGTCGC TTTCTACGTA TTGCAGGCTG CCCTGATGAT CTCACTCATT 
TGGAAGTATT ATTCTGTCCC TGTGGCTGTC GTGCCGAGTA AATGGATAAC CCCTCTAGAC 
CGCCTGGTAG CCTTTCCTAC TAGAGTAGCA GGTGGTGTTG GAATTACCTG TTGGATTTTA 
GTCTGTAACA AAGTTGTCGC TATTGTGCTT CATCCGTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GET1 ( NM_001350294.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337223.1 Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu01966
Clone ID Related Accession (Same CDS sequence)	NM_001350294.1 , NM_001350296.1 , NM_001350295.1 , NM_001146218.2
Accession Version	NM_001350294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	tail-anchored protein insertion receptor WRB isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF064861.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: ERR279833.5096.1, SRR1660807.411822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337223.1
CDS	100..522
Translation	MSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGVGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_001350294.1
Organism	Homo sapiens(human)
Definition	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350294.1

ORF Insert Sequence:

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GET1 ( NM_001350296.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337225.1 Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu01966
Clone ID Related Accession (Same CDS sequence)	NM_001350294.1 , NM_001350296.1 , NM_001350295.1 , NM_001146218.2
Accession Version	NM_001350296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	tail-anchored protein insertion receptor WRB isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF064861.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.36887.1, SRR1803612.113865.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337225.1
CDS	465..887
Translation	MSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGVGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_001350296.1
Organism	Homo sapiens(human)
Definition	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350296.1

ORF Insert Sequence:

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GET1 ( NM_001350295.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337224.1 Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu01966
Clone ID Related Accession (Same CDS sequence)	NM_001350294.1 , NM_001350296.1 , NM_001350295.1 , NM_001146218.2
Accession Version	NM_001350295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	tail-anchored protein insertion receptor WRB isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF064861.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.201556.1, SRR1660805.229622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337224.1
CDS	190..612
Translation	MSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGVGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_001350295.1
Organism	Homo sapiens(human)
Definition	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350295.1

ORF Insert Sequence:

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GET1 ( NM_001146218.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139690.1 Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01966
Clone ID Related Accession (Same CDS sequence)	NM_001350294.1 , NM_001350296.1 , NM_001350295.1 , NM_001146218.2
Accession Version	NM_001146218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	tail-anchored protein insertion receptor WRB isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF064861.5, DB498697.1, AK299144.1 and AW001614.1. On Apr 13, 2017 this sequence version replaced NM_001146218.1. Transcript Variant: This variant (2) represents use of an alternate promoter compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.304557.1, BC111393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139690.1
CDS	94..516
Translation	MSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGVGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_001146218.2
Organism	Homo sapiens(human)
Definition	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146218.2

ORF Insert Sequence:

ATGTCCAGGG TGCTGCAGAA GGACGCGGAG CAGGAGTCAC AGATGAGAGC GGAGATCCAG 
GACATGAAGC AGGAGCTCTC CACAGTCAAC ATGATGGACG AGTTTGCCAG ATATGCCAGG 
CTGGAAAGAA AGATCAACAA GATGACGGAT AAGCTCAAAA CCCATGTGAA AGCTCGGACA 
GCTCAATTAG CCAAGATAAA ATGGGTGATA AGTGTCGCTT TCTACGTATT GCAGGCTGCC 
CTGATGATCT CACTCATTTG GAAGTATTAT TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA 
TGGATAACCC CTCTAGACCG CCTGGTAGCC TTTCCTACTA GAGTAGCAGG TGGTGTTGGA 
ATTACCTGTT GGATTTTAGT CTGTAACAAA GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GET1 ( NM_001350293.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337222.1 Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101318
Clone ID Related Accession (Same CDS sequence)	NM_001350293.1
Accession Version	NM_001350293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	tail-anchored protein insertion receptor WRB isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF064861.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.269545.1, SRR1163655.99042.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCCC AGTCCCCCGG CGGGTCTGGC ATGTCCAGGG TGCTGCAGAA GGACGCGGAG 
CAGGAGTCAC AGATGAGAGC GGAGATCCAG GACATGAAGC AGGAGCTCTC CACAGTCAAC 
ATGATGGACG AGTTTGCCAG ATATGCCAGG CTGGAAAGAA AGATCAACAA GATGACGGAT 
AAGCTCAAAA CCCATGTGAA AGCTCGGACA GCTCAATTAG CCAAGATAAA ATGGGTGATA 
AGTGTCGCTT TCTACGTATT GCAGGCTGCC CTGATGATCT CACTCATTTG GAAGTATTAT 
TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA TGGATAACCC CTCTAGACCG CCTGGTAGCC 
TTTCCTACTA GAGTAGCAGG TGGTGTTGGA ATTACCTGTT GGATTTTAGT CTGTAACAAA 
GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337222.1
CDS	66..518
Translation	MPPQSPGGSGMSRVLQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIWKYYSVPVAVVPSKWITPLDRLVAFPTRVAGGVGITCWILVCNKVVAIVLHPFS

Target ORF information:

RefSeq Version	NM_001350293.1
Organism	Homo sapiens(human)
Definition	Homo sapiens guided entry of tail-anchored proteins factor 1 (GET1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350293.1

ORF Insert Sequence:

ATGCCGCCCC AGTCCCCCGG CGGGTCTGGC ATGTCCAGGG TGCTGCAGAA GGACGCGGAG 
CAGGAGTCAC AGATGAGAGC GGAGATCCAG GACATGAAGC AGGAGCTCTC CACAGTCAAC 
ATGATGGACG AGTTTGCCAG ATATGCCAGG CTGGAAAGAA AGATCAACAA GATGACGGAT 
AAGCTCAAAA CCCATGTGAA AGCTCGGACA GCTCAATTAG CCAAGATAAA ATGGGTGATA 
AGTGTCGCTT TCTACGTATT GCAGGCTGCC CTGATGATCT CACTCATTTG GAAGTATTAT 
TCTGTCCCTG TGGCTGTCGT GCCGAGTAAA TGGATAACCC CTCTAGACCG CCTGGTAGCC 
TTTCCTACTA GAGTAGCAGG TGGTGTTGGA ATTACCTGTT GGATTTTAGT CTGTAACAAA 
GTTGTCGCTA TTGTGCTTCA TCCGTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Trisomy 21 Alters DNA Methylation in Parent-of-Origin-Dependent and -Independent Manners.
PloS one11(4)e0154108(2016)
Alves da Silva AF,Machado FB,Pavarino ?C,Biselli-P?rico JM,Zampieri BL,da Silva Francisco Junior R,Mozer Rodrigues PT,Terra Machado D,Santos-Rebou?as CB,Gomes Fernandes M,Chuva de Sousa Lopes SM,Lopes Rios ?F,Medina-Acosta E

Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.
Molecular cell48(3)387-97(2012 Nov)
Yamamoto Y,Sakisaka T

WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.
Journal of cell science124(Pt 8)1301-7(2011 Apr)
Vilardi F,Lorenz H,Dobberstein B

Identification of a transcriptionally active peroxisome proliferator-activated receptor alpha -interacting cofactor complex in rat liver and characterization of PRIC285 as a coactivator.
Proceedings of the National Academy of Sciences of the United States of America99(18)11836-41(2002 Sep)
Surapureddi S,Yu S,Bu H,Hashimoto T,Yeldandi AV,Kashireddy P,Cherkaoui-Malki M,Qi C,Zhu YJ,Rao MS,Reddy JK

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

GET1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報