n4bp1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	n4bp1
Entrez Gene ID	327327
Full Name	nedd4 binding protein 1
Synonyms	wu:fi03b05,zgc:66437
General protein information	Preferred Names nedd4 binding protein 1 Names NEDD4-binding protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199787.2	NP_956081.2	NEDD4-binding protein 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	N4bp1	XP_214648.5
Pan troglodytes (chimpanzee)	N4BP1	XP_520627.3
Canis lupus familiaris (dog)	N4BP1	XP_005617644.1
	N4BP1	XP_001109863.2
Gallus gallus (chicken)	N4BP1	NP_001025741.1
Danio rerio (zebrafish)	n4bp1	NP_956081.1
Mus musculus (house mouse)	N4bp1	NP_085040.2
Homo sapiens (human)	N4BP1	NP_694574.3
Bos taurus (cattle)	N4BP1	NP_001178362.1
Xenopus tropicalis (tropical clawed frog)	n4bp1	NP_001004870.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Smith LC, Clark JC, Bisesi JH Jr, Ferguson PL, Sabo-Attwood T
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Wang Y, Zhang S
Functional & integrative genomics11(1)111-8(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following n4bp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the n4bp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53244	NM_199787.2 Latest version!	Danio rerio nedd4 binding protein 1 (n4bp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

n4bp1 ( NM_199787.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956081.2 Danio rerio nedd4 binding protein 1 (n4bp1), mRNA.

Price & Availability↑

CloneID	ODa53244
Clone ID Related Accession (Same CDS sequence)	NM_199787.2
Accession Version	NM_199787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	NEDD4-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649644.5. On Jan 29, 2016 this sequence version replaced NM_199787.1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC059205.1, GDQH01006596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCAACAA CGCGGCCCCT CCTCGGGATG AAGCGAATCA CCGAGGTAAC CTGCACGGAG 
CCGCCGGGAG GCAGACAGTC ACCGACCGCC AGCAGAGCAC AACCCGATTC GCTCACCGTA 
GACGAGTTTA CAGTGCACGA AGACAAGCAG ACCGAGCTCA AATGCAGCAA ACCGAAGGTC 
GAACAGGTTT TCCAAGTGAC ATTCACGATT ATCGGGCTCC TGGATCATAC TGGAGCCCAC 
GGAAGTAAGG CTTCCAGACA GATCTGGCTC CAGCTTAAAG GGAAAAAGGA GGATGTGTAT 
AAAGCGAAGG AGTATGTGAA AGGCCTGTGT GACCCAGAGC TTCAGAAGGA AGAATGGTAC 
CCTGTGGACA TGCACTGCAT TTTCGCTGGT GCTCGAGGAC TCTTTTTGGA CCGGCTGCTT 
AGAGACACAA GTGCAGAGGT CCAAGTTCTA GAGCCAGGCC GCCTGAAGTT GAGCGGGTGT 
GCAGAGGCCG TTGTCATGGC TCAAAGTCGG GTTCAACAGT TTGTCGCTTT ATTTCAGGAG 
AAGCGAAGTT TGCCAGCTGA CAGGGAGCCA TCAGTTAAGC GCAAATTTAA GACTTTTGTT 
GAGGACCGGG CTGATAAATA TGCCATGGAG CTGCTGTTGC TGCCAAGTGC CCTCAAAGAG 
GAACTTTTGG GTTTGGCCCA AAGCCCCACA CAGCCTATAG TTATCATAGA CCTTGAACAA 
GACCGCTCTC AGACTAGCAC ACCTGTAACA GACCTCTCCA ACCGTATCCT AGACACAACT 
TTTGAGGACA AAACCTCTCC AATCACCCCT GAGGTCATGC CAGGTCTGAA TGGCCGGCCT 
TGCAACAAGC GGCGGTCCTC TGAAAGTGAG CAGAGGGACA CTAAAAGACA ATATTCGCTG 
GAGAGGCGAG AAGAGGAGCA GTGTGAAGAG CGTGAGCGAG AGCCAACTAA AACTTGGACT 
GTAAAGTCTG CCAAGGGGAC GTTGGCTGCT AGTGAAATGA CAAATGAAAG TGAGGCGGTA 
AGTCCTGAGA CTAACTTACG CTGTCTCGTT AACTTTTTTC GAACGATGGG CTACCAGCAG 
GATGTGGTGG AGCGGGTGGT GCGGGAGACA GGGCAGACGG AGGACACATT TTTACTGCTA 
GAACGAATTG TTGAAGAGAC TCAGAAAACC CAGAGCACAC AAGGAGCCCA GCGCACCTCT 
CGCACACCCG ATCCATCCCC TTGTGCAAAT GCCTCATCCA CCTCCACCAG CAACAGGCTC 
AAAGAGAAGG AGCGGGTGCA GATGCGCGCT CTCGCTGAGA TTAAGTGCAA AGAAAACATC 
AGACCTCCAA GCACTAATGG CATCGGTCAG AAGAACCAGA CAAGCAGTGT GCCACTGGCC 
TCGGCCACTC TTAAAAGAAA CAATGGTGCA CAGACTGACT TGTGTGAAGT GATCATCATC 
GATGATGAAG AGGACTTCAC AGAAACCGAG AGGAAGCCCA GACTGACCCC ACTTGATTTA 
AAACCAGAGT CCCGCTTTGA TTACCTGCCA CGTGGTAGCT CTCAAACAAT GGTTCCTGTG 
CGTATGGAGA CTGTTACCAA TCTTCGCAGC TCCTCTCAAG GTCCTCCTCT CCGGACCTCA 
GACACACGGC CGGGCTGCTC TTACCAGACA CTCCCTGGAA GGGCACCTTT ACCTCGCTCA 
GAGGCACAAT ACACCTCTAA AGCAGCCCCT CTCACCGGAA TGTCTCGTTT TCAGCAGTCC 
CTGAGAACCC CCTACCGACT AATCCTACAA AATGAACCAG GGTCCCCTAA TCTGCGGCAC 
ATCATCATCG ATGGCAGCAA TGTGGCAATG GCGCATGGAC TCCATCGGGT CTTTTCTTGT 
CGTGGAATTG CCATCGCTGT AGAGGCATTT TGGCGCAGAG GACATCGAGA AATCACTGTG 
TTTGTTCCTC AGTGGAGACA GAAGAAAGAC CCCAACATTA CTGAGCAACA CTTTCTGAAC 
CAGCTGGAAA ACCTGCGACT TCTTTCTTTC ACCCCATCTA GAGAAGTGTG TGGACATAGA 
ATTTCCTCCC ATGATGACAG GTTCCTGCTT CACCTGGCTG AGAAGACTGG AGGAGTGATT 
GTCACCAATG ACAACCTGAG GGACTTTGTG AGCCAGTCAG AAGCATGGAG AAGGATTATT 
CATGAGAGAC TCTTGCAGTT CACCTTTGTA GAAGATCACT TTATGATCCC CGATGACCCA 
CTTGGAAAAC ACGGGCCTCA CCTTGACGAG TTTCTACTTA AGGACAGCCG AGGTAGTCCT 
ATAATCCCTC CTCTGAGAAC AGACCTTCGG GCAACCCCGT CAGTTTACAG CCAGGCAGCA 
CAGTCCACAG CACACCCCTC TTCCCCTTCA CACTGGCCAC ACTCGGGCCC ACCAGACTGG 
CACCTTCCTA GACCGTCGCC ATCCCCACCC CCACAGCGGT CACCCTCAGA AACCACAGAG 
CTCAAGAGGA AGTTATACGA CATCTTTCCT GACCAAAAGC AGCGTATTGA CCGTATCCTC 
AGCGACAACC CTTATATGAG GGACCTAAAT GCATTATCGG GCCTTTTGCT AGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956081.2
CDS	201..2777
Translation	MSTTRPLLGMKRITEVTCTEPPGGRQSPTASRAQPDSLTVDEFTVHEDKQTELKCSKPKVEQVFQVTFTIIGLLDHTGAHGSKASRQIWLQLKGKKEDVYKAKEYVKGLCDPELQKEEWYPVDMHCIFAGARGLFLDRLLRDTSAEVQVLEPGRLKLSGCAEAVVMAQSRVQQFVALFQEKRSLPADREPSVKRKFKTFVEDRADKYAMELLLLPSALKEELLGLAQSPTQPIVIIDLEQDRSQTSTPVTDLSNRILDTTFEDKTSPITPEVMPGLNGRPCNKRRSSESEQRDTKRQYSLERREEEQCEEREREPTKTWTVKSAKGTLAASEMTNESEAVSPETNLRCLVNFFRTMGYQQDVVERVVRETGQTEDTFLLLERIVEETQKTQSTQGAQRTSRTPDPSPCANASSTSTSNRLKEKERVQMRALAEIKCKENIRPPSTNGIGQKNQTSSVPLASATLKRNNGAQTDLCEVIIIDDEEDFTETERKPRLTPLDLKPESRFDYLPRGSSQTMVPVRMETVTNLRSSSQGPPLRTSDTRPGCSYQTLPGRAPLPRSEAQYTSKAAPLTGMSRFQQSLRTPYRLILQNEPGSPNLRHIIIDGSNVAMAHGLHRVFSCRGIAIAVEAFWRRGHREITVFVPQWRQKKDPNITEQHFLNQLENLRLLSFTPSREVCGHRISSHDDRFLLHLAEKTGGVIVTNDNLRDFVSQSEAWRRIIHERLLQFTFVEDHFMIPDDPLGKHGPHLDEFLLKDSRGSPIIPPLRTDLRATPSVYSQAAQSTAHPSSPSHWPHSGPPDWHLPRPSPSPPPQRSPSETTELKRKLYDIFPDQKQRIDRILSDNPYMRDLNALSGLLLG

Target ORF information:

RefSeq Version	NM_199787.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nedd4 binding protein 1 (n4bp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199787.2

ORF Insert Sequence:

ATGTCAACAA CGCGGCCCCT CCTCGGGATG AAGCGAATCA CCGAGGTAAC CTGCACGGAG 
CCGCCGGGAG GCAGACAGTC ACCGACCGCC AGCAGAGCAC AACCCGATTC GCTCACCGTA 
GACGAGTTTA CAGTGCACGA AGACAAGCAG ACCGAGCTCA AATGCAGCAA ACCGAAGGTC 
GAACAGGTTT TCCAAGTGAC ATTCACGATT ATCGGGCTCC TGGATCATAC TGGAGCCCAC 
GGAAGTAAGG CTTCCAGACA GATCTGGCTC CAGCTTAAAG GGAAAAAGGA GGATGTGTAT 
AAAGCGAAGG AGTATGTGAA AGGCCTGTGT GACCCAGAGC TTCAGAAGGA AGAATGGTAC 
CCTGTGGACA TGCACTGCAT TTTCGCTGGT GCTCGAGGAC TCTTTTTGGA CCGGCTGCTT 
AGAGACACAA GTGCAGAGGT CCAAGTTCTA GAGCCAGGCC GCCTGAAGTT GAGCGGGTGT 
GCAGAGGCCG TTGTCATGGC TCAAAGTCGG GTTCAACAGT TTGTCGCTTT ATTTCAGGAG 
AAGCGAAGTT TGCCAGCTGA CAGGGAGCCA TCAGTTAAGC GCAAATTTAA GACTTTTGTT 
GAGGACCGGG CTGATAAATA TGCCATGGAG CTGCTGTTGC TGCCAAGTGC CCTCAAAGAG 
GAACTTTTGG GTTTGGCCCA AAGCCCCACA CAGCCTATAG TTATCATAGA CCTTGAACAA 
GACCGCTCTC AGACTAGCAC ACCTGTAACA GACCTCTCCA ACCGTATCCT AGACACAACT 
TTTGAGGACA AAACCTCTCC AATCACCCCT GAGGTCATGC CAGGTCTGAA TGGCCGGCCT 
TGCAACAAGC GGCGGTCCTC TGAAAGTGAG CAGAGGGACA CTAAAAGACA ATATTCGCTG 
GAGAGGCGAG AAGAGGAGCA GTGTGAAGAG CGTGAGCGAG AGCCAACTAA AACTTGGACT 
GTAAAGTCTG CCAAGGGGAC GTTGGCTGCT AGTGAAATGA CAAATGAAAG TGAGGCGGTA 
AGTCCTGAGA CTAACTTACG CTGTCTCGTT AACTTTTTTC GAACGATGGG CTACCAGCAG 
GATGTGGTGG AGCGGGTGGT GCGGGAGACA GGGCAGACGG AGGACACATT TTTACTGCTA 
GAACGAATTG TTGAAGAGAC TCAGAAAACC CAGAGCACAC AAGGAGCCCA GCGCACCTCT 
CGCACACCCG ATCCATCCCC TTGTGCAAAT GCCTCATCCA CCTCCACCAG CAACAGGCTC 
AAAGAGAAGG AGCGGGTGCA GATGCGCGCT CTCGCTGAGA TTAAGTGCAA AGAAAACATC 
AGACCTCCAA GCACTAATGG CATCGGTCAG AAGAACCAGA CAAGCAGTGT GCCACTGGCC 
TCGGCCACTC TTAAAAGAAA CAATGGTGCA CAGACTGACT TGTGTGAAGT GATCATCATC 
GATGATGAAG AGGACTTCAC AGAAACCGAG AGGAAGCCCA GACTGACCCC ACTTGATTTA 
AAACCAGAGT CCCGCTTTGA TTACCTGCCA CGTGGTAGCT CTCAAACAAT GGTTCCTGTG 
CGTATGGAGA CTGTTACCAA TCTTCGCAGC TCCTCTCAAG GTCCTCCTCT CCGGACCTCA 
GACACACGGC CGGGCTGCTC TTACCAGACA CTCCCTGGAA GGGCACCTTT ACCTCGCTCA 
GAGGCACAAT ACACCTCTAA AGCAGCCCCT CTCACCGGAA TGTCTCGTTT TCAGCAGTCC 
CTGAGAACCC CCTACCGACT AATCCTACAA AATGAACCAG GGTCCCCTAA TCTGCGGCAC 
ATCATCATCG ATGGCAGCAA TGTGGCAATG GCGCATGGAC TCCATCGGGT CTTTTCTTGT 
CGTGGAATTG CCATCGCTGT AGAGGCATTT TGGCGCAGAG GACATCGAGA AATCACTGTG 
TTTGTTCCTC AGTGGAGACA GAAGAAAGAC CCCAACATTA CTGAGCAACA CTTTCTGAAC 
CAGCTGGAAA ACCTGCGACT TCTTTCTTTC ACCCCATCTA GAGAAGTGTG TGGACATAGA 
ATTTCCTCCC ATGATGACAG GTTCCTGCTT CACCTGGCTG AGAAGACTGG AGGAGTGATT 
GTCACCAATG ACAACCTGAG GGACTTTGTG AGCCAGTCAG AAGCATGGAG AAGGATTATT 
CATGAGAGAC TCTTGCAGTT CACCTTTGTA GAAGATCACT TTATGATCCC CGATGACCCA 
CTTGGAAAAC ACGGGCCTCA CCTTGACGAG TTTCTACTTA AGGACAGCCG AGGTAGTCCT 
ATAATCCCTC CTCTGAGAAC AGACCTTCGG GCAACCCCGT CAGTTTACAG CCAGGCAGCA 
CAGTCCACAG CACACCCCTC TTCCCCTTCA CACTGGCCAC ACTCGGGCCC ACCAGACTGG 
CACCTTCCTA GACCGTCGCC ATCCCCACCC CCACAGCGGT CACCCTCAGA AACCACAGAG 
CTCAAGAGGA AGTTATACGA CATCTTTCCT GACCAAAAGC AGCGTATTGA CCGTATCCTC 
AGCGACAACC CTTATATGAG GGACCTAAAT GCATTATCGG GCCTTTTGCT AGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)
Smith LC,Clark JC,Bisesi JH Jr,Ferguson PL,Sabo-Attwood T

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Functional & integrative genomics11(1)111-8(2011 Mar)
Wang Y,Zhang S