OTUD4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	OTUD4
Entrez Gene ID	327127
Full Name	OTU deubiquitinase 4
Synonyms	wu:fd14a11,wu:fe11h08,zgc:165536
General protein information	Preferred Names OTU deubiquitinase 4 Names OTU domain-containing protein 4 OTU domain containing 4 protein parp11
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001922973.6	XP_001923008.4	OTU domain-containing protein 4 isoform X2
XM_005174151.4	XP_005174208.2	OTU domain-containing protein 4 isoform X1
XM_021469751.1	XP_021325426.1	OTU domain-containing protein 4 isoform X3
XM_017353521.2	XP_017209010.2	OTU domain-containing protein 4 isoform X4
NM_001312659.3	NP_001299588.3	OTU domain-containing protein 4
NM_001312659.1	NP_001299588.1	OTU domain-containing protein 4
XM_005174151.3	XP_005174208.1	OTU domain-containing protein 4 isoform X1
XM_017353521.1	XP_017209010.1	OTU domain-containing protein 4 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish transforming growth factor-?-stimulated clone 22 domain 3 (TSC22D3) plays critical roles in Bmp-dependent dorsoventral patterning via two deubiquitylating enzymes Usp15 and Otud4.
Tse WK, Jiang YJ, Wong CK
Biochimica et biophysica acta1830(10)4584-93(2013 Oct)

Ataxia, dementia, and hypogonadotropism caused by disordered ubiquitination.
Margolin DH, Kousi M, Chan YM, Lim ET, Schmahmann JD, Hadjivassiliou M, Hall JE, Adam I, Dwyer A, Plummer L, Aldrin SV, O'Rourke J, Kirby A, Lage K, Milunsky A, Milunsky JM, Chan J, Hedley-Whyte ET, Daly MJ, Katsanis N, Seminara SB
The New England journal of medicine368(21)1992-2003(2013 May)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interruption of Bmp4 signaling by double knockdown of usp15 and otud4 reduced the ventralized effects in tsc22d3-overexpressing embryos
Title: Zebrafish transforming growth factor-?-stimulated clone 22 domain 3 (TSC22D3) plays critical roles in Bmp-dependent dorsoventral patterning via two deubiquitylating enzymes Usp15 and Otud4.

The following OTUD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTUD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151443	XM_017353521.2 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa57342	XM_017353521.1 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa141189	NM_001312659.3 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa28117	NM_001312659.1 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa151441	XM_005174151.4 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa151442	XM_021469751.1 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa28118	XM_005174151.3 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa28119	XM_001922973.6 Latest version!	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OTUD4 ( XM_017353521.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209010.2 Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa151443
Clone ID Related Accession (Same CDS sequence)	XM_017353521.2
Accession Version	XM_017353521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	OTU domain-containing protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGCA GACGGTCTGA TCAGCGCAGC 
AGTCCTGTGT GTATTCTGCC AGAAGAGGAA GAGGAAAGCG TTGTTTTGGA GCTTCTGCAC 
AAAGATGAGC ACAACTTCCC CTCACTTGGA CCCAGCCCCC AAACGGCCAC TCCTGGTGAG 
GTCATAAAGA AAGGAGGGGA AAAGAAAAGC CATCGGAAAA AGGAACAGAA GGAGAACTTC 
ATGGAGACAG AAGCATCTCA GAAGCCTTTA CAGAGGCAGA AGAGTGGGAC TGAAGAAAAA 
CATGGGGTGC AGCGCCTGTT GTTGACCACC CATCCCAACC CCAACACCTG CCCTTTCCTC 
AAGTCCAGTG GTCTCAGCTC CTACAGGACC CAGTTTATCC AGGATTTCCA CAGAATGAGA 
AGGGACAGTT GGAACCACCT CCACCGTTTT CCTACTCCCA GAAAGGAGAG GACCTACCGA 
AAGATATTAG TGTCCTCAAG TTCTTCTTCA ACCTTGGGGT CAAGGCTTAC ACCCAGCCAA 
TGTTTCCTCC TATTGTATAT CTGGCACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209010.2
CDS	425..2197
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLNPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNGAQIPSEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFSRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGVQRLLLTTHPNPNTCPFLKSSGLSSYRTQFIQDFHRMRRDSWNHLHRFPTPRKERTYRKILVSSSSSSTLGSRLTPSQCFLLLYIWHH

Target ORF information:

RefSeq Version	XM_017353521.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353521.2

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGCA GACGGTCTGA TCAGCGCAGC 
AGTCCTGTGT GTATTCTGCC AGAAGAGGAA GAGGAAAGCG TTGTTTTGGA GCTTCTGCAC 
AAAGATGAGC ACAACTTCCC CTCACTTGGA CCCAGCCCCC AAACGGCCAC TCCTGGTGAG 
GTCATAAAGA AAGGAGGGGA AAAGAAAAGC CATCGGAAAA AGGAACAGAA GGAGAACTTC 
ATGGAGACAG AAGCATCTCA GAAGCCTTTA CAGAGGCAGA AGAGTGGGAC TGAAGAAAAA 
CATGGGGTGC AGCGCCTGTT GTTGACCACC CATCCCAACC CCAACACCTG CCCTTTCCTC 
AAGTCCAGTG GTCTCAGCTC CTACAGGACC CAGTTTATCC AGGATTTCCA CAGAATGAGA 
AGGGACAGTT GGAACCACCT CCACCGTTTT CCTACTCCCA GAAAGGAGAG GACCTACCGA 
AAGATATTAG TGTCCTCAAG TTCTTCTTCA ACCTTGGGGT CAAGGCTTAC ACCCAGCCAA 
TGTTTCCTCC TATTGTATAT CTGGCACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTUD4 ( XM_017353521.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209010.1 Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57342
Clone ID Related Accession (Same CDS sequence)	XM_017353521.1
Accession Version	XM_017353521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	OTU domain-containing protein 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGCA GACGGTCTGA TCAGCGCAGC 
AGTCCTGTGT GTATTCTGCC AGAAGAGGAA GAGGAAAGCG TTGTTTTGGA GCTTCTGCAC 
AAAGATGAGC ACAACTTCCC CTCACTTGGA CCCAGCCCCC AAACGGCCAC TCCTGGTGAG 
GTCATAAAGA AAGGAGGGGA AAAGAAAAGC CATCGGAAAA AGGAACAGAA GGAGAACTTC 
ATGGAGACAG AAGCATCTCA GAAGCCTTTA CAGAGGCAGA AGAGTGGGAC TGAAGAAAAA 
CATGGGGTGC AGCGCCTGTT GTTGACCACC CATCCCAACC CCAACACCTG CCCTTTCCTC 
AAGTCCAGTG GTCTCAGCTC CTACAGGACC CAGTTTATCC AGGATTTCCA CAGAATGAGA 
AGGGACAGTT GGAACCACCT CCACCGTTTT CCTACTCCCA GAAAGGAGAG GACCTACCGA 
AAGATATTAG TGTCCTCAAG TTCTTCTTCA ACCTTGGGGT CAAGGCTTAC ACCCAGCCAA 
TGTTTCCTCC TATTGTATAT CTGGCACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209010.1
CDS	294..2066
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLSPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNGAQIPSEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFSRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGVQRLLLTTHPNPNTCPFLKSSGLSSYRTQFIQDFHRMRRDSWNHLHRFPTPRKERTYRKILVSSSSSSTLGSRLTPSQCFLLLYIWHH

Target ORF information:

RefSeq Version	XM_017353521.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353521.1

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGCA GACGGTCTGA TCAGCGCAGC 
AGTCCTGTGT GTATTCTGCC AGAAGAGGAA GAGGAAAGCG TTGTTTTGGA GCTTCTGCAC 
AAAGATGAGC ACAACTTCCC CTCACTTGGA CCCAGCCCCC AAACGGCCAC TCCTGGTGAG 
GTCATAAAGA AAGGAGGGGA AAAGAAAAGC CATCGGAAAA AGGAACAGAA GGAGAACTTC 
ATGGAGACAG AAGCATCTCA GAAGCCTTTA CAGAGGCAGA AGAGTGGGAC TGAAGAAAAA 
CATGGGGTGC AGCGCCTGTT GTTGACCACC CATCCCAACC CCAACACCTG CCCTTTCCTC 
AAGTCCAGTG GTCTCAGCTC CTACAGGACC CAGTTTATCC AGGATTTCCA CAGAATGAGA 
AGGGACAGTT GGAACCACCT CCACCGTTTT CCTACTCCCA GAAAGGAGAG GACCTACCGA 
AAGATATTAG TGTCCTCAAG TTCTTCTTCA ACCTTGGGGT CAAGGCTTAC ACCCAGCCAA 
TGTTTCCTCC TATTGTATAT CTGGCACCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTUD4 ( NM_001312659.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001299588.3 Danio rerio OTU deubiquitinase 4 (otud4), mRNA.

Price & Availability↑

CloneID	ODa141189
Clone ID Related Accession (Same CDS sequence)	NM_001312659.3
Accession Version	NM_001312659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	OTU domain-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01058306.1, FO704781.1 and CU681840.13. On or before Jul 27, 2017 this sequence version replaced XM_001922973.7, NM_001312659.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGAATGGGA AAGCAGAGGA GGTCGGAGGC CAAACAGAAT GCAGTCTCAG 
TCGGCACCCA GCGTTCGTGT ACATAAGCAA CACTCCTGGC CTCCGCAGGC CACAACTGAT 
GATGCAAGAA ACAAGTTCAG CAGACGGTCT GATCAGCGCA GCAGTCCTGT GTGTATTCTG 
CCAGAAGAGG AAGAGGAAAG CGTTGTTTTG GAGCTTCTGC ACAAAGATGA GCACAACTTC 
CCCTCACTTG GACCCAGCCC CCAAACGGCC ACTCCTGGTG AGGTCATAAA GAAAGGAGGG 
GAAAAGAAAA GCCATCGGAA AAAGGAACAG AAGGAGAACT TCATGGAGAC AGAAGCATCT 
CAGAAGCCTT TACAGAGGCA GAAGAGTGGG ACTGAAGAAA AACATGGGAT AAAGTTTTCT 
GAGGAACCAA GGCAAAGGTC TTCTTCAACA ATAAACGAGA AATTACAAGA GAAACCTATC 
CCTGTCGCTG CTTCTCCACC TTCACCTGCT ATCTTCTTAT CTGGTCCCAA AACACCCATT 
TCTTCATCTA ATTCTGCCAC TTCCCCTGAT CCTAACCGGT CTGCTCCTGA GGCAGTTAAA 
AAGGCAACCA TCCAACCTAA GTCTAGCCCT GCTCAAAACC CTGACAACAG TCATTTGGAA 
CCAGTGTCAA CTTTAGCCTC TTTACAGCAC ATCTCTAGGG TAGCACCAGT CTCAGAAACC 
ACTCCTGCCT CGCAGTTTAT CTCTGGTGCA GCACCAGTGT CAGTTGTCCC ACCTGCCTCA 
CAGTCTATCT CTGCCACCAC TCCTGCCCCA CAGTCTATCT CTGCTGCAGC ACCAGTTTCT 
GTTTCCACCC CTGCCTCAGA CTCTATACTT GTGGCAGCAC CAGACTCAGC TACCACCCCT 
GCCTCACAGT ATATCCCTGG GTCAAGGGCA GTCTCAGTTA GCACCCCTGC CCCACAGTCT 
ATCTCTGGGG CAACAGCAGT CTCATCTAAC ACCCCTACCC CACACTCCAT CTCTAGGGCA 
ACAGCAGTCT CAGTTACTGC TCCTGCTCCA CACTTCACCT TTGTGGCAAC AGCAGAATCC 
ATTTCCACCC CTTCCCCACA CTCTATCTCT GGAGCAACAG CAGTTGCCAC CCCTGCTCCT 
ATTCAATCTG CACCTGCACT AAATTCTACC AAACCCTCAT TCCAAATGGC CCAATTGCCT 
ACAGTGGAGC TCCTGCCCAG AACTGAAGCA CCATCTCAGT TCACCTCGGT TGAATTGGTT 
GCATCTCAGA TCACCCTAGT CCCTTTACCT ACTTCTACCC CAACCCAAAC TACTCTGGCC 
CCTACTTGCC CAATCCCAGC TGCCCCAGTT GAATCCAGCT TGTGCATTCA AACTTTGCCA 
CCAGCCAATA CTTCTACTCT ATCCCCAACA CCATCATCCA CAATACCGAT TTCTGCACAG 
GCTCAGGTTG CTACCCCTGT ATTAGATTCC CCAGGCTTGT CTTCTACCCC ACATCCTATT 
CATGCAAACA ATGCTCCACC AATTCCCAAA AAGACTGTTG ATCCTCAAAC CACTATGATG 
CCAGTCCCTC TTCCCCATGC TGCTCCCACC TCTATCTCCC CATCTATTGG TTCTGCTAAT 
GCTTCACCTC CTGACTTACC TCTAGTCTCT CCAAAAGAAT CAGTCCCATC TGCTTGTGAA 
ACATCAACCA TACCTGCCTC TGGTGCAGCG CCTGTTGTTG ACCACCCATC CCAACCCCAA 
CACCTGCCCT TTCCTCAAGT CCAGTGGTCT CAGCTCCTAC AGGACCCAGT TTATCCAGGA 
TTTCCACAGA ATGAGAAGGG ACAGTTGGAA CCACCTCCAC CGTTTTCCTA CTCCCAGAAA 
GGAGAGGACC TACCGAAAGA TATTAGTGTC CTCAAGTTCT TCTTCAACCT TGGGGTCAAG 
GCTTACACCC AGCCAATGTT TCCTCCTATT GTATATCTGG CACCACTGAA CCAAGCCTAC 
CTTAGAGGTC CTTCTCCTTC CTCTGACTCT CCTGCTAATC CCACCATAAC TTCGTGGCAG 
CCAGAAAACC CTTCTTCTCA GCCTCAAAGT TTGAGCTCCA TGGTTGACAC CCCATTGGAC 
AGCCATGCTC CAATTGCACC TGTAGCAGCT GGTAGCAGTC CTGTTGATCC CATAGAGGTA 
AGAGGATACA ACGATCCTCC ATCGATTCCA ATGCATGTGC CACCAAGACC ACCAATGCCT 
TGGTCAGCTC CTTCTGTTTC AAGTGCCTAT CCTGGGGGTT ACCCAACTAC TCCTCCAGTG 
CAGTTTTCCA CACCTCCATA TCCACCCCCT GGGAATCAGA TGTATCCACC ATCATCTGTT 
GGATACCATC GTATGCCATT TCCACCAATG CCTTTAGAAG CAATAAACCA TGGGCCTCAT 
GGTCCGATTG ATGTCTTGCC TTTTGCTCGC CCCACACTTG AAAGAGGTCA AGAGAATGGT 
CAGTTGTCAA TAAACCCCCA ATTTAGGTCT GTACAGAACT CTGCTGGCTT TCCTGGAGGC 
CAACCTCATT TTTTGAGTGG TGTAGATTTC AAGTCAGTAG ATACTCCTGT TTCTCTGGGT 
GGAATGGAAC CTCCTCCTTA TGGTGGACAC TGTTCGGTAC CATCTTTTCA GATTGGCCCA 
TTGCAAGGAG ACATGTCAGG ATTGATCCCT TTACCAAATG GGAGCTTAGG AAGACAAGGG 
GGGGCCTTCC CGAAGAACCT ACATGCTGAA GACTCTTCCC GCCTCGTCCA TACATTTTCT 
CCTGAAGAAA AGTGGCCAGA GGAAATGGAG TCCACCAATG TAGACTTGAG AGTTCCTCAG 
TTATACTACA GCCATTCCTA CAGGGGTGGA GGAAGGCGTG GCCAAGAGGA CAGAGGAGGA 
TATCGAGGGC GGAGCCAACG TGGACGGAAG GATTATATAG GCCGAGGCAG ACAAGACGAC 
CAGTCCTACA GACATTACGG GAGACGAGGT GCAGGGCCAC AGTTGCCATA TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299588.3
CDS	247..4323
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLNPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFSRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGIKFSEEPRQRSSSTINEKLQEKPIPVAASPPSPAIFLSGPKTPISSSNSATSPDPNRSAPEAVKKATIQPKSSPAQNPDNSHLEPVSTLASLQHISRVAPVSETTPASQFISGAAPVSVVPPASQSISATTPAPQSISAAAPVSVSTPASDSILVAAPDSATTPASQYIPGSRAVSVSTPAPQSISGATAVSSNTPTPHSISRATAVSVTAPAPHFTFVATAESISTPSPHSISGATAVATPAPIQSAPALNSTKPSFQMAQLPTVELLPRTEAPSQFTSVELVASQITLVPLPTSTPTQTTLAPTCPIPAAPVESSLCIQTLPPANTSTLSPTPSSTIPISAQAQVATPVLDSPGLSSTPHPIHANNAPPIPKKTVDPQTTMMPVPLPHAAPTSISPSIGSANASPPDLPLVSPKESVPSACETSTIPASGAAPVVDHPSQPQHLPFPQVQWSQLLQDPVYPGFPQNEKGQLEPPPPFSYSQKGEDLPKDISVLKFFFNLGVKAYTQPMFPPIVYLAPLNQAYLRGPSPSSDSPANPTITSWQPENPSSQPQSLSSMVDTPLDSHAPIAPVAAGSSPVDPIEVRGYNDPPSIPMHVPPRPPMPWSAPSVSSAYPGGYPTTPPVQFSTPPYPPPGNQMYPPSSVGYHRMPFPPMPLEAINHGPHGPIDVLPFARPTLERGQENGQLSINPQFRSVQNSAGFPGGQPHFLSGVDFKSVDTPVSLGGMEPPPYGGHCSVPSFQIGPLQGDMSGLIPLPNGSLGRQGGAFPKNLHAEDSSRLVHTFSPEEKWPEEMESTNVDLRVPQLYYSHSYRGGGRRGQEDRGGYRGRSQRGRKDYIGRGRQDDQSYRHYGRRGAGPQLPYQ

Target ORF information:

RefSeq Version	NM_001312659.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312659.3

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGAATGGGA AAGCAGAGGA GGTCGGAGGC CAAACAGAAT GCAGTCTCAG 
TCGGCACCCA GCGTTCGTGT ACATAAGCAA CACTCCTGGC CTCCGCAGGC CACAACTGAT 
GATGCAAGAA ACAAGTTCAG CAGACGGTCT GATCAGCGCA GCAGTCCTGT GTGTATTCTG 
CCAGAAGAGG AAGAGGAAAG CGTTGTTTTG GAGCTTCTGC ACAAAGATGA GCACAACTTC 
CCCTCACTTG GACCCAGCCC CCAAACGGCC ACTCCTGGTG AGGTCATAAA GAAAGGAGGG 
GAAAAGAAAA GCCATCGGAA AAAGGAACAG AAGGAGAACT TCATGGAGAC AGAAGCATCT 
CAGAAGCCTT TACAGAGGCA GAAGAGTGGG ACTGAAGAAA AACATGGGAT AAAGTTTTCT 
GAGGAACCAA GGCAAAGGTC TTCTTCAACA ATAAACGAGA AATTACAAGA GAAACCTATC 
CCTGTCGCTG CTTCTCCACC TTCACCTGCT ATCTTCTTAT CTGGTCCCAA AACACCCATT 
TCTTCATCTA ATTCTGCCAC TTCCCCTGAT CCTAACCGGT CTGCTCCTGA GGCAGTTAAA 
AAGGCAACCA TCCAACCTAA GTCTAGCCCT GCTCAAAACC CTGACAACAG TCATTTGGAA 
CCAGTGTCAA CTTTAGCCTC TTTACAGCAC ATCTCTAGGG TAGCACCAGT CTCAGAAACC 
ACTCCTGCCT CGCAGTTTAT CTCTGGTGCA GCACCAGTGT CAGTTGTCCC ACCTGCCTCA 
CAGTCTATCT CTGCCACCAC TCCTGCCCCA CAGTCTATCT CTGCTGCAGC ACCAGTTTCT 
GTTTCCACCC CTGCCTCAGA CTCTATACTT GTGGCAGCAC CAGACTCAGC TACCACCCCT 
GCCTCACAGT ATATCCCTGG GTCAAGGGCA GTCTCAGTTA GCACCCCTGC CCCACAGTCT 
ATCTCTGGGG CAACAGCAGT CTCATCTAAC ACCCCTACCC CACACTCCAT CTCTAGGGCA 
ACAGCAGTCT CAGTTACTGC TCCTGCTCCA CACTTCACCT TTGTGGCAAC AGCAGAATCC 
ATTTCCACCC CTTCCCCACA CTCTATCTCT GGAGCAACAG CAGTTGCCAC CCCTGCTCCT 
ATTCAATCTG CACCTGCACT AAATTCTACC AAACCCTCAT TCCAAATGGC CCAATTGCCT 
ACAGTGGAGC TCCTGCCCAG AACTGAAGCA CCATCTCAGT TCACCTCGGT TGAATTGGTT 
GCATCTCAGA TCACCCTAGT CCCTTTACCT ACTTCTACCC CAACCCAAAC TACTCTGGCC 
CCTACTTGCC CAATCCCAGC TGCCCCAGTT GAATCCAGCT TGTGCATTCA AACTTTGCCA 
CCAGCCAATA CTTCTACTCT ATCCCCAACA CCATCATCCA CAATACCGAT TTCTGCACAG 
GCTCAGGTTG CTACCCCTGT ATTAGATTCC CCAGGCTTGT CTTCTACCCC ACATCCTATT 
CATGCAAACA ATGCTCCACC AATTCCCAAA AAGACTGTTG ATCCTCAAAC CACTATGATG 
CCAGTCCCTC TTCCCCATGC TGCTCCCACC TCTATCTCCC CATCTATTGG TTCTGCTAAT 
GCTTCACCTC CTGACTTACC TCTAGTCTCT CCAAAAGAAT CAGTCCCATC TGCTTGTGAA 
ACATCAACCA TACCTGCCTC TGGTGCAGCG CCTGTTGTTG ACCACCCATC CCAACCCCAA 
CACCTGCCCT TTCCTCAAGT CCAGTGGTCT CAGCTCCTAC AGGACCCAGT TTATCCAGGA 
TTTCCACAGA ATGAGAAGGG ACAGTTGGAA CCACCTCCAC CGTTTTCCTA CTCCCAGAAA 
GGAGAGGACC TACCGAAAGA TATTAGTGTC CTCAAGTTCT TCTTCAACCT TGGGGTCAAG 
GCTTACACCC AGCCAATGTT TCCTCCTATT GTATATCTGG CACCACTGAA CCAAGCCTAC 
CTTAGAGGTC CTTCTCCTTC CTCTGACTCT CCTGCTAATC CCACCATAAC TTCGTGGCAG 
CCAGAAAACC CTTCTTCTCA GCCTCAAAGT TTGAGCTCCA TGGTTGACAC CCCATTGGAC 
AGCCATGCTC CAATTGCACC TGTAGCAGCT GGTAGCAGTC CTGTTGATCC CATAGAGGTA 
AGAGGATACA ACGATCCTCC ATCGATTCCA ATGCATGTGC CACCAAGACC ACCAATGCCT 
TGGTCAGCTC CTTCTGTTTC AAGTGCCTAT CCTGGGGGTT ACCCAACTAC TCCTCCAGTG 
CAGTTTTCCA CACCTCCATA TCCACCCCCT GGGAATCAGA TGTATCCACC ATCATCTGTT 
GGATACCATC GTATGCCATT TCCACCAATG CCTTTAGAAG CAATAAACCA TGGGCCTCAT 
GGTCCGATTG ATGTCTTGCC TTTTGCTCGC CCCACACTTG AAAGAGGTCA AGAGAATGGT 
CAGTTGTCAA TAAACCCCCA ATTTAGGTCT GTACAGAACT CTGCTGGCTT TCCTGGAGGC 
CAACCTCATT TTTTGAGTGG TGTAGATTTC AAGTCAGTAG ATACTCCTGT TTCTCTGGGT 
GGAATGGAAC CTCCTCCTTA TGGTGGACAC TGTTCGGTAC CATCTTTTCA GATTGGCCCA 
TTGCAAGGAG ACATGTCAGG ATTGATCCCT TTACCAAATG GGAGCTTAGG AAGACAAGGG 
GGGGCCTTCC CGAAGAACCT ACATGCTGAA GACTCTTCCC GCCTCGTCCA TACATTTTCT 
CCTGAAGAAA AGTGGCCAGA GGAAATGGAG TCCACCAATG TAGACTTGAG AGTTCCTCAG 
TTATACTACA GCCATTCCTA CAGGGGTGGA GGAAGGCGTG GCCAAGAGGA CAGAGGAGGA 
TATCGAGGGC GGAGCCAACG TGGACGGAAG GATTATATAG GCCGAGGCAG ACAAGACGAC 
CAGTCCTACA GACATTACGG GAGACGAGGT GCAGGGCCAC AGTTGCCATA TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTUD4 ( NM_001312659.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001299588.1 Danio rerio OTU deubiquitinase 4 (otud4), mRNA.

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CloneID	ODa28117
Clone ID Related Accession (Same CDS sequence)	NM_001312659.1
Accession Version	NM_001312659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-26
Organism	Danio rerio(Zebrafish)
Product	OTU domain-containing protein 4
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01058306.1 and CU681840.13. On Aug 14, 2015 this sequence version replaced XM_005174150.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGCA GACGGTCTGA TCAGCGCAGC 
AGTCCTGTGT GTATTCTGCC AGAAGAGGAA GAGGAAAGCG TTGTTTTGGA GCTTCTGCAC 
AAAGATGAGC ACAACTTCCC CTCACTTGGA CCCAGCCCCC AAACGGCCAC TCCTGGTGAG 
GTCATAAAGA AAGGAGGGGA AAAGAAAAGC CATCGGAAAA AGGAACAGAA GGAGAACTTC 
ATGGAGACAG AAGCATCTCA GAAGCCTTTA CAGAGGCAGA AGAGTGGGAC TGAAGAAAAA 
CATGGGATAA AGTTTTCTGA GGAACCAAGG CAAAGGTCTT CTTCAACAAT AAACGAGAAA 
TTACAAGAGA AACCTATCCC TGTCGCTGCT TCTCCACCTT CACCTGCTAT CTTCTTATCT 
GGTCCCAAAA CACCCATTTC TTCATCTAAT TCTGCCACTT CCCCTGATCC TAACCGGTCT 
GCTCCTGAGG CAGTTAAAAA GGCAACCATC CAACCTAAGT CTAGCCCTGC TCAAAACCCT 
GACAACAGTC ATTTGGAACC AGTGTCAACT TTAGCCTCTT TACAGCACAT CTCTAGGGTA 
GCACCAGTCT CAGAAACCAC TCCTGCCTCG CAGTTTATCT CTGGTGCAGC ACCAGTGTCA 
GTTGTCCCAC CTGCCTCACA GTCTATCTCT GCCACCACTC CTGCCCCACA GTCTATCTCT 
GCTGCAGCAC CAGTTTCTGT TTCCACCCCT GCCTCAGACT CTATACTTGT GGCAGCACCA 
GACTCAGCTA CCACCCCTGC CTCACAGTAT ATCCCTGGGT CAAGGGCAGT CTCAGTTAGC 
ACCCCTGCCC CACAGTCTAT CTCTGGGGCA ACAGCAGTCT CATCTAACAC CCCTACCCCA 
CACTCCATCT CTAGGGCAAC AGCAGTCTCA GTTACTGCTC CTGCTCCACA CTTCACCTTT 
GTGGCAACAG CAGAATCCAT TTCCACCCCT TCCCCACACT CTATCTCTGG AGCAACAGCA 
GTTGCCACCC CTGCTCCTAT TCAATCTGCA CCTGCACTAA ATTCTACCAA ACCCTCATTC 
CAAATGGCCC AATTGCCTAC AGTGGAGCTC CTGCCCAGAA CTGAAGCACC ATCTCAGTTC 
ACCTCGGTTG AATTGGTTGC ATCTCAGATC ACCCTAGTCC CTTTACCTAC TTCTACCCCA 
ACCCAAACTA CTCTGGCCCC TACTTGCCCA ATCCCAGCTG CCCCAGTTGA ATCCAGCTTG 
TGCATTCAAA CTTTGCCACC AGCCAATACT TCTACTCTAT CCCCAACACC ATCATCCACA 
ATACCGATTT CTGCACAGGC TCAGGTTGCT ACCCCTGTAT TAGATTCCCC AGGCTTGTCT 
TCTACCCCAC ATCCTATTCA TGCAAACAAT GCTCCACCAA TTCCCAAAAA GACTGTTGAT 
CCTCAAACCA CTATGATGCC AGTCCCTCTT CCCCATGCTG CTCCCACCTC TATCTCCCCA 
TCTATTGGTT CTGCTAATGC TTCACCTCCT GACTTACCTC TAGTCTCTCC AAAAGAATCA 
GTCCCATCTG CTTGTGAAAC ATCAACCATA CCTGCCTCTG GTGCAGCGCC TGTTGTTGAC 
CACCCATCCC AACCCCAACA CCTGCCCTTT CCTCAAGTCC AGTGGTCTCA GCTCCTACAG 
GACCCAGTTT ATCCAGGATT TCCACAGAAT GAGAAGGGAC AGTTGGAACC ACCTCCACCG 
TTTTCCTACT CCCAGAAAGG AGAGGACCTA CCGAAAGATA TTAGTGTCCT CAAGTTCTTC 
TTCAACCTTG GGGTCAAGGC TTACACCCAG CCAATGTTTC CTCCTATTGT ATATCTGGCA 
CCACTGAACC AAGCCTACCT TAGAGGTCCT TCTCCTTCCT CTGACTCTCC TGCTAATCCC 
ACCATAACTT CGTGGCAGCC AGAAAACCCT TCTTCTCAGC CTCAAAGTTT GAGCTCCATG 
GTTGACACCC CATTGGACAG CCATGCTCCA ATTGCACCTG TAGCAGCTGG TAGCAGTCCT 
GTTGATCCCA TAGAGGTAAG AGGATACAAC GATCCTCCAT CGATTCCAAT GCATGTGCCA 
CCAAGACCAC CAATGCCTTG GTCAGCTCCT TCTGTTTCAA GTGCCTATCC TGGGGGTTAC 
CCAACTACTC CTCCAGTGCA GTTTTCCACA CCTCCATATC CACCCCCTGG GAATCAGATG 
TATCCACCAT CATCTGTTGG ATACCATCGT ATGCCATTTC CACCAATGCC TTTAGAAGCA 
ATAAACCATG GGCCTCATGG TCCGATTGAT GTCTTGCCTT TTGCTCGCCC CACACTTGAA 
AGAGGTCAAG AGAATGGTCA GTTGTCAATA AACCCCCAAT TTAGGTCTGT ACAGAACTCT 
GCTGGCTTTC CTGGAGGCCA ACCTCATTTT TTGAGTGGTG TAGATTTCAA GTCAGTAGAT 
ACTCCTGTTT CTCTGGGTGG AATGGAACCT CCTCCTTATG GTGGACACTG TTCGGTACCA 
TCTTTTCAGA TTGGCCCATT GCAAGGAGAC ATGTCAGGAT TGATCCCTTT ACCAAATGGG 
AGCTTAGGAA GACAAGGGGG GGCCTTCCCG AAGAACCTAC ATGCTGAAGA CTCTTCCCGC 
CTCGTCCATA CATTTTCTCC TGAAGAAAAG TGGCCAGAGG AAATGGAGTC CACCAATGTA 
GACTTGAGAG TTCCTCAGTT ATACTACAGC CATTCCTACA GGGGTGGAGG AAGGCGTGGC 
CAAGAGGACA GAGGAGGATA TCGAGGGCGG AGCCAACGTG GACGGAAGGA TTATATAGGC 
CGAGGCAGAC AAGACGACCA GTCCTACAGA CATTACGGGA GACGAGGTGC AGGGCCACAG 
TTGCCATATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299588.1
CDS	363..4457
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLSPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNGAQIPSEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFSRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGIKFSEEPRQRSSSTINEKLQEKPIPVAASPPSPAIFLSGPKTPISSSNSATSPDPNRSAPEAVKKATIQPKSSPAQNPDNSHLEPVSTLASLQHISRVAPVSETTPASQFISGAAPVSVVPPASQSISATTPAPQSISAAAPVSVSTPASDSILVAAPDSATTPASQYIPGSRAVSVSTPAPQSISGATAVSSNTPTPHSISRATAVSVTAPAPHFTFVATAESISTPSPHSISGATAVATPAPIQSAPALNSTKPSFQMAQLPTVELLPRTEAPSQFTSVELVASQITLVPLPTSTPTQTTLAPTCPIPAAPVESSLCIQTLPPANTSTLSPTPSSTIPISAQAQVATPVLDSPGLSSTPHPIHANNAPPIPKKTVDPQTTMMPVPLPHAAPTSISPSIGSANASPPDLPLVSPKESVPSACETSTIPASGAAPVVDHPSQPQHLPFPQVQWSQLLQDPVYPGFPQNEKGQLEPPPPFSYSQKGEDLPKDISVLKFFFNLGVKAYTQPMFPPIVYLAPLNQAYLRGPSPSSDSPANPTITSWQPENPSSQPQSLSSMVDTPLDSHAPIAPVAAGSSPVDPIEVRGYNDPPSIPMHVPPRPPMPWSAPSVSSAYPGGYPTTPPVQFSTPPYPPPGNQMYPPSSVGYHRMPFPPMPLEAINHGPHGPIDVLPFARPTLERGQENGQLSINPQFRSVQNSAGFPGGQPHFLSGVDFKSVDTPVSLGGMEPPPYGGHCSVPSFQIGPLQGDMSGLIPLPNGSLGRQGGAFPKNLHAEDSSRLVHTFSPEEKWPEEMESTNVDLRVPQLYYSHSYRGGGRRGQEDRGGYRGRSQRGRKDYIGRGRQDDQSYRHYGRRGAGPQLPYQ

Target ORF information:

RefSeq Version	NM_001312659.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312659.1

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGCA GACGGTCTGA TCAGCGCAGC 
AGTCCTGTGT GTATTCTGCC AGAAGAGGAA GAGGAAAGCG TTGTTTTGGA GCTTCTGCAC 
AAAGATGAGC ACAACTTCCC CTCACTTGGA CCCAGCCCCC AAACGGCCAC TCCTGGTGAG 
GTCATAAAGA AAGGAGGGGA AAAGAAAAGC CATCGGAAAA AGGAACAGAA GGAGAACTTC 
ATGGAGACAG AAGCATCTCA GAAGCCTTTA CAGAGGCAGA AGAGTGGGAC TGAAGAAAAA 
CATGGGATAA AGTTTTCTGA GGAACCAAGG CAAAGGTCTT CTTCAACAAT AAACGAGAAA 
TTACAAGAGA AACCTATCCC TGTCGCTGCT TCTCCACCTT CACCTGCTAT CTTCTTATCT 
GGTCCCAAAA CACCCATTTC TTCATCTAAT TCTGCCACTT CCCCTGATCC TAACCGGTCT 
GCTCCTGAGG CAGTTAAAAA GGCAACCATC CAACCTAAGT CTAGCCCTGC TCAAAACCCT 
GACAACAGTC ATTTGGAACC AGTGTCAACT TTAGCCTCTT TACAGCACAT CTCTAGGGTA 
GCACCAGTCT CAGAAACCAC TCCTGCCTCG CAGTTTATCT CTGGTGCAGC ACCAGTGTCA 
GTTGTCCCAC CTGCCTCACA GTCTATCTCT GCCACCACTC CTGCCCCACA GTCTATCTCT 
GCTGCAGCAC CAGTTTCTGT TTCCACCCCT GCCTCAGACT CTATACTTGT GGCAGCACCA 
GACTCAGCTA CCACCCCTGC CTCACAGTAT ATCCCTGGGT CAAGGGCAGT CTCAGTTAGC 
ACCCCTGCCC CACAGTCTAT CTCTGGGGCA ACAGCAGTCT CATCTAACAC CCCTACCCCA 
CACTCCATCT CTAGGGCAAC AGCAGTCTCA GTTACTGCTC CTGCTCCACA CTTCACCTTT 
GTGGCAACAG CAGAATCCAT TTCCACCCCT TCCCCACACT CTATCTCTGG AGCAACAGCA 
GTTGCCACCC CTGCTCCTAT TCAATCTGCA CCTGCACTAA ATTCTACCAA ACCCTCATTC 
CAAATGGCCC AATTGCCTAC AGTGGAGCTC CTGCCCAGAA CTGAAGCACC ATCTCAGTTC 
ACCTCGGTTG AATTGGTTGC ATCTCAGATC ACCCTAGTCC CTTTACCTAC TTCTACCCCA 
ACCCAAACTA CTCTGGCCCC TACTTGCCCA ATCCCAGCTG CCCCAGTTGA ATCCAGCTTG 
TGCATTCAAA CTTTGCCACC AGCCAATACT TCTACTCTAT CCCCAACACC ATCATCCACA 
ATACCGATTT CTGCACAGGC TCAGGTTGCT ACCCCTGTAT TAGATTCCCC AGGCTTGTCT 
TCTACCCCAC ATCCTATTCA TGCAAACAAT GCTCCACCAA TTCCCAAAAA GACTGTTGAT 
CCTCAAACCA CTATGATGCC AGTCCCTCTT CCCCATGCTG CTCCCACCTC TATCTCCCCA 
TCTATTGGTT CTGCTAATGC TTCACCTCCT GACTTACCTC TAGTCTCTCC AAAAGAATCA 
GTCCCATCTG CTTGTGAAAC ATCAACCATA CCTGCCTCTG GTGCAGCGCC TGTTGTTGAC 
CACCCATCCC AACCCCAACA CCTGCCCTTT CCTCAAGTCC AGTGGTCTCA GCTCCTACAG 
GACCCAGTTT ATCCAGGATT TCCACAGAAT GAGAAGGGAC AGTTGGAACC ACCTCCACCG 
TTTTCCTACT CCCAGAAAGG AGAGGACCTA CCGAAAGATA TTAGTGTCCT CAAGTTCTTC 
TTCAACCTTG GGGTCAAGGC TTACACCCAG CCAATGTTTC CTCCTATTGT ATATCTGGCA 
CCACTGAACC AAGCCTACCT TAGAGGTCCT TCTCCTTCCT CTGACTCTCC TGCTAATCCC 
ACCATAACTT CGTGGCAGCC AGAAAACCCT TCTTCTCAGC CTCAAAGTTT GAGCTCCATG 
GTTGACACCC CATTGGACAG CCATGCTCCA ATTGCACCTG TAGCAGCTGG TAGCAGTCCT 
GTTGATCCCA TAGAGGTAAG AGGATACAAC GATCCTCCAT CGATTCCAAT GCATGTGCCA 
CCAAGACCAC CAATGCCTTG GTCAGCTCCT TCTGTTTCAA GTGCCTATCC TGGGGGTTAC 
CCAACTACTC CTCCAGTGCA GTTTTCCACA CCTCCATATC CACCCCCTGG GAATCAGATG 
TATCCACCAT CATCTGTTGG ATACCATCGT ATGCCATTTC CACCAATGCC TTTAGAAGCA 
ATAAACCATG GGCCTCATGG TCCGATTGAT GTCTTGCCTT TTGCTCGCCC CACACTTGAA 
AGAGGTCAAG AGAATGGTCA GTTGTCAATA AACCCCCAAT TTAGGTCTGT ACAGAACTCT 
GCTGGCTTTC CTGGAGGCCA ACCTCATTTT TTGAGTGGTG TAGATTTCAA GTCAGTAGAT 
ACTCCTGTTT CTCTGGGTGG AATGGAACCT CCTCCTTATG GTGGACACTG TTCGGTACCA 
TCTTTTCAGA TTGGCCCATT GCAAGGAGAC ATGTCAGGAT TGATCCCTTT ACCAAATGGG 
AGCTTAGGAA GACAAGGGGG GGCCTTCCCG AAGAACCTAC ATGCTGAAGA CTCTTCCCGC 
CTCGTCCATA CATTTTCTCC TGAAGAAAAG TGGCCAGAGG AAATGGAGTC CACCAATGTA 
GACTTGAGAG TTCCTCAGTT ATACTACAGC CATTCCTACA GGGGTGGAGG AAGGCGTGGC 
CAAGAGGACA GAGGAGGATA TCGAGGGCGG AGCCAACGTG GACGGAAGGA TTATATAGGC 
CGAGGCAGAC AAGACGACCA GTCCTACAGA CATTACGGGA GACGAGGTGC AGGGCCACAG 
TTGCCATATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTUD4 ( XM_005174151.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174208.2 Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X1, mRNA.

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CloneID	ODa151441
Clone ID Related Accession (Same CDS sequence)	XM_005174151.4
Accession Version	XM_005174151.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	OTU domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174151.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGAC GGTCTGATCA GCGCAGCAGT 
CCTGTGTGTA TTCTGCCAGA AGAGGAAGAG GAAAGCGTTG TTTTGGAGCT TCTGCACAAA 
GATGAGCACA ACTTCCCCTC ACTTGGACCC AGCCCCCAAA CGGCCACTCC TGGTGAGGTC 
ATAAAGAAAG GAGGGGAAAA GAAAAGCCAT CGGAAAAAGG AACAGAAGGA GAACTTCATG 
GAGACAGAAG CATCTCAGAA GCCTTTACAG AGGCAGAAGA GTGGGACTGA AGAAAAACAT 
GGGATAAAGT TTTCTGAGGA ACCAAGGCAA AGGTCTTCTT CAACAATAAA CGAGAAATTA 
CAAGAGAAAC CTATCCCTGT CGCTGCTTCT CCACCTTCAC CTGCTATCTT CTTATCTGGT 
CCCAAAACAC CCATTTCTTC ATCTAATTCT GCCACTTCCC CTGATCCTAA CCGGTCTGCT 
CCTGAGGCAG TTAAAAAGGC AACCATCCAA CCTAAGTCTA GCCCTGCTCA AAACCCTGAC 
AACAGTCATT TGGAACCAGT GTCAACTTTA GCCTCTTTAC AGCACATCTC TAGGGTAGCA 
CCAGTCTCAG AAACCACTCC TGCCTCGCAG TTTATCTCTG GTGCAGCACC AGTGTCAGTT 
GTCCCACCTG CCTCACAGTC TATCTCTGCC ACCACTCCTG CCCCACAGTC TATCTCTGCT 
GCAGCACCAG TTTCTGTTTC CACCCCTGCC TCAGACTCTA TACTTGTGGC AGCACCAGAC 
TCAGCTACCA CCCCTGCCTC ACAGTATATC CCTGGGTCAA GGGCAGTCTC AGTTAGCACC 
CCTGCCCCAC AGTCTATCTC TGGGGCAACA GCAGTCTCAT CTAACACCCC TACCCCACAC 
TCCATCTCTA GGGCAACAGC AGTCTCAGTT ACTGCTCCTG CTCCACACTT CACCTTTGTG 
GCAACAGCAG AATCCATTTC CACCCCTTCC CCACACTCTA TCTCTGGAGC AACAGCAGTT 
GCCACCCCTG CTCCTATTCA ATCTGCACCT GCACTAAATT CTACCAAACC CTCATTCCAA 
ATGGCCCAAT TGCCTACAGT GGAGCTCCTG CCCAGAACTG AAGCACCATC TCAGTTCACC 
TCGGTTGAAT TGGTTGCATC TCAGATCACC CTAGTCCCTT TACCTACTTC TACCCCAACC 
CAAACTACTC TGGCCCCTAC TTGCCCAATC CCAGCTGCCC CAGTTGAATC CAGCTTGTGC 
ATTCAAACTT TGCCACCAGC CAATACTTCT ACTCTATCCC CAACACCATC ATCCACAATA 
CCGATTTCTG CACAGGCTCA GGTTGCTACC CCTGTATTAG ATTCCCCAGG CTTGTCTTCT 
ACCCCACATC CTATTCATGC AAACAATGCT CCACCAATTC CCAAAAAGAC TGTTGATCCT 
CAAACCACTA TGATGCCAGT CCCTCTTCCC CATGCTGCTC CCACCTCTAT CTCCCCATCT 
ATTGGTTCTG CTAATGCTTC ACCTCCTGAC TTACCTCTAG TCTCTCCAAA AGAATCAGTC 
CCATCTGCTT GTGAAACATC AACCATACCT GCCTCTGGTG CAGCGCCTGT TGTTGACCAC 
CCATCCCAAC CCCAACACCT GCCCTTTCCT CAAGTCCAGT GGTCTCAGCT CCTACAGGAC 
CCAGTTTATC CAGGATTTCC ACAGAATGAG AAGGGACAGT TGGAACCACC TCCACCGTTT 
TCCTACTCCC AGAAAGGAGA GGACCTACCG AAAGATATTA GTGTCCTCAA GTTCTTCTTC 
AACCTTGGGG TCAAGGCTTA CACCCAGCCA ATGTTTCCTC CTATTGTATA TCTGGCACCA 
CTGAACCAAG CCTACCTTAG AGGTCCTTCT CCTTCCTCTG ACTCTCCTGC TAATCCCACC 
ATAACTTCGT GGCAGCCAGA AAACCCTTCT TCTCAGCCTC AAAGTTTGAG CTCCATGGTT 
GACACCCCAT TGGACAGCCA TGCTCCAATT GCACCTGTAG CAGCTGGTAG CAGTCCTGTT 
GATCCCATAG AGGTAAGAGG ATACAACGAT CCTCCATCGA TTCCAATGCA TGTGCCACCA 
AGACCACCAA TGCCTTGGTC AGCTCCTTCT GTTTCAAGTG CCTATCCTGG GGGTTACCCA 
ACTACTCCTC CAGTGCAGTT TTCCACACCT CCATATCCAC CCCCTGGGAA TCAGATGTAT 
CCACCATCAT CTGTTGGATA CCATCGTATG CCATTTCCAC CAATGCCTTT AGAAGCAATA 
AACCATGGGC CTCATGGTCC GATTGATGTC TTGCCTTTTG CTCGCCCCAC ACTTGAAAGA 
GGTCAAGAGA ATGGTCAGTT GTCAATAAAC CCCCAATTTA GGTCTGTACA GAACTCTGCT 
GGCTTTCCTG GAGGCCAACC TCATTTTTTG AGTGGTGTAG ATTTCAAGTC AGTAGATACT 
CCTGTTTCTC TGGGTGGAAT GGAACCTCCT CCTTATGGTG GACACTGTTC GGTACCATCT 
TTTCAGATTG GCCCATTGCA AGGAGACATG TCAGGATTGA TCCCTTTACC AAATGGGAGC 
TTAGGAAGAC AAGGGGGGGC CTTCCCGAAG AACCTACATG CTGAAGACTC TTCCCGCCTC 
GTCCATACAT TTTCTCCTGA AGAAAAGTGG CCAGAGGAAA TGGAGTCCAC CAATGTAGAC 
TTGAGAGTTC CTCAGTTATA CTACAGCCAT TCCTACAGGG GTGGAGGAAG GCGTGGCCAA 
GAGGACAGAG GAGGATATCG AGGGCGGAGC CAACGTGGAC GGAAGGATTA TATAGGCCGA 
GGCAGACAAG ACGACCAGTC CTACAGACAT TACGGGAGAC GAGGTGCAGG GCCACAGTTG 
CCATATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174208.2
CDS	414..4505
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLNPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNGAQIPSEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGIKFSEEPRQRSSSTINEKLQEKPIPVAASPPSPAIFLSGPKTPISSSNSATSPDPNRSAPEAVKKATIQPKSSPAQNPDNSHLEPVSTLASLQHISRVAPVSETTPASQFISGAAPVSVVPPASQSISATTPAPQSISAAAPVSVSTPASDSILVAAPDSATTPASQYIPGSRAVSVSTPAPQSISGATAVSSNTPTPHSISRATAVSVTAPAPHFTFVATAESISTPSPHSISGATAVATPAPIQSAPALNSTKPSFQMAQLPTVELLPRTEAPSQFTSVELVASQITLVPLPTSTPTQTTLAPTCPIPAAPVESSLCIQTLPPANTSTLSPTPSSTIPISAQAQVATPVLDSPGLSSTPHPIHANNAPPIPKKTVDPQTTMMPVPLPHAAPTSISPSIGSANASPPDLPLVSPKESVPSACETSTIPASGAAPVVDHPSQPQHLPFPQVQWSQLLQDPVYPGFPQNEKGQLEPPPPFSYSQKGEDLPKDISVLKFFFNLGVKAYTQPMFPPIVYLAPLNQAYLRGPSPSSDSPANPTITSWQPENPSSQPQSLSSMVDTPLDSHAPIAPVAAGSSPVDPIEVRGYNDPPSIPMHVPPRPPMPWSAPSVSSAYPGGYPTTPPVQFSTPPYPPPGNQMYPPSSVGYHRMPFPPMPLEAINHGPHGPIDVLPFARPTLERGQENGQLSINPQFRSVQNSAGFPGGQPHFLSGVDFKSVDTPVSLGGMEPPPYGGHCSVPSFQIGPLQGDMSGLIPLPNGSLGRQGGAFPKNLHAEDSSRLVHTFSPEEKWPEEMESTNVDLRVPQLYYSHSYRGGGRRGQEDRGGYRGRSQRGRKDYIGRGRQDDQSYRHYGRRGAGPQLPYQ

Target ORF information:

RefSeq Version	XM_005174151.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174151.4

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGAC GGTCTGATCA GCGCAGCAGT 
CCTGTGTGTA TTCTGCCAGA AGAGGAAGAG GAAAGCGTTG TTTTGGAGCT TCTGCACAAA 
GATGAGCACA ACTTCCCCTC ACTTGGACCC AGCCCCCAAA CGGCCACTCC TGGTGAGGTC 
ATAAAGAAAG GAGGGGAAAA GAAAAGCCAT CGGAAAAAGG AACAGAAGGA GAACTTCATG 
GAGACAGAAG CATCTCAGAA GCCTTTACAG AGGCAGAAGA GTGGGACTGA AGAAAAACAT 
GGGATAAAGT TTTCTGAGGA ACCAAGGCAA AGGTCTTCTT CAACAATAAA CGAGAAATTA 
CAAGAGAAAC CTATCCCTGT CGCTGCTTCT CCACCTTCAC CTGCTATCTT CTTATCTGGT 
CCCAAAACAC CCATTTCTTC ATCTAATTCT GCCACTTCCC CTGATCCTAA CCGGTCTGCT 
CCTGAGGCAG TTAAAAAGGC AACCATCCAA CCTAAGTCTA GCCCTGCTCA AAACCCTGAC 
AACAGTCATT TGGAACCAGT GTCAACTTTA GCCTCTTTAC AGCACATCTC TAGGGTAGCA 
CCAGTCTCAG AAACCACTCC TGCCTCGCAG TTTATCTCTG GTGCAGCACC AGTGTCAGTT 
GTCCCACCTG CCTCACAGTC TATCTCTGCC ACCACTCCTG CCCCACAGTC TATCTCTGCT 
GCAGCACCAG TTTCTGTTTC CACCCCTGCC TCAGACTCTA TACTTGTGGC AGCACCAGAC 
TCAGCTACCA CCCCTGCCTC ACAGTATATC CCTGGGTCAA GGGCAGTCTC AGTTAGCACC 
CCTGCCCCAC AGTCTATCTC TGGGGCAACA GCAGTCTCAT CTAACACCCC TACCCCACAC 
TCCATCTCTA GGGCAACAGC AGTCTCAGTT ACTGCTCCTG CTCCACACTT CACCTTTGTG 
GCAACAGCAG AATCCATTTC CACCCCTTCC CCACACTCTA TCTCTGGAGC AACAGCAGTT 
GCCACCCCTG CTCCTATTCA ATCTGCACCT GCACTAAATT CTACCAAACC CTCATTCCAA 
ATGGCCCAAT TGCCTACAGT GGAGCTCCTG CCCAGAACTG AAGCACCATC TCAGTTCACC 
TCGGTTGAAT TGGTTGCATC TCAGATCACC CTAGTCCCTT TACCTACTTC TACCCCAACC 
CAAACTACTC TGGCCCCTAC TTGCCCAATC CCAGCTGCCC CAGTTGAATC CAGCTTGTGC 
ATTCAAACTT TGCCACCAGC CAATACTTCT ACTCTATCCC CAACACCATC ATCCACAATA 
CCGATTTCTG CACAGGCTCA GGTTGCTACC CCTGTATTAG ATTCCCCAGG CTTGTCTTCT 
ACCCCACATC CTATTCATGC AAACAATGCT CCACCAATTC CCAAAAAGAC TGTTGATCCT 
CAAACCACTA TGATGCCAGT CCCTCTTCCC CATGCTGCTC CCACCTCTAT CTCCCCATCT 
ATTGGTTCTG CTAATGCTTC ACCTCCTGAC TTACCTCTAG TCTCTCCAAA AGAATCAGTC 
CCATCTGCTT GTGAAACATC AACCATACCT GCCTCTGGTG CAGCGCCTGT TGTTGACCAC 
CCATCCCAAC CCCAACACCT GCCCTTTCCT CAAGTCCAGT GGTCTCAGCT CCTACAGGAC 
CCAGTTTATC CAGGATTTCC ACAGAATGAG AAGGGACAGT TGGAACCACC TCCACCGTTT 
TCCTACTCCC AGAAAGGAGA GGACCTACCG AAAGATATTA GTGTCCTCAA GTTCTTCTTC 
AACCTTGGGG TCAAGGCTTA CACCCAGCCA ATGTTTCCTC CTATTGTATA TCTGGCACCA 
CTGAACCAAG CCTACCTTAG AGGTCCTTCT CCTTCCTCTG ACTCTCCTGC TAATCCCACC 
ATAACTTCGT GGCAGCCAGA AAACCCTTCT TCTCAGCCTC AAAGTTTGAG CTCCATGGTT 
GACACCCCAT TGGACAGCCA TGCTCCAATT GCACCTGTAG CAGCTGGTAG CAGTCCTGTT 
GATCCCATAG AGGTAAGAGG ATACAACGAT CCTCCATCGA TTCCAATGCA TGTGCCACCA 
AGACCACCAA TGCCTTGGTC AGCTCCTTCT GTTTCAAGTG CCTATCCTGG GGGTTACCCA 
ACTACTCCTC CAGTGCAGTT TTCCACACCT CCATATCCAC CCCCTGGGAA TCAGATGTAT 
CCACCATCAT CTGTTGGATA CCATCGTATG CCATTTCCAC CAATGCCTTT AGAAGCAATA 
AACCATGGGC CTCATGGTCC GATTGATGTC TTGCCTTTTG CTCGCCCCAC ACTTGAAAGA 
GGTCAAGAGA ATGGTCAGTT GTCAATAAAC CCCCAATTTA GGTCTGTACA GAACTCTGCT 
GGCTTTCCTG GAGGCCAACC TCATTTTTTG AGTGGTGTAG ATTTCAAGTC AGTAGATACT 
CCTGTTTCTC TGGGTGGAAT GGAACCTCCT CCTTATGGTG GACACTGTTC GGTACCATCT 
TTTCAGATTG GCCCATTGCA AGGAGACATG TCAGGATTGA TCCCTTTACC AAATGGGAGC 
TTAGGAAGAC AAGGGGGGGC CTTCCCGAAG AACCTACATG CTGAAGACTC TTCCCGCCTC 
GTCCATACAT TTTCTCCTGA AGAAAAGTGG CCAGAGGAAA TGGAGTCCAC CAATGTAGAC 
TTGAGAGTTC CTCAGTTATA CTACAGCCAT TCCTACAGGG GTGGAGGAAG GCGTGGCCAA 
GAGGACAGAG GAGGATATCG AGGGCGGAGC CAACGTGGAC GGAAGGATTA TATAGGCCGA 
GGCAGACAAG ACGACCAGTC CTACAGACAT TACGGGAGAC GAGGTGCAGG GCCACAGTTG 
CCATATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTUD4 ( XM_021469751.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325426.1 Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa151442
Clone ID Related Accession (Same CDS sequence)	XM_021469751.1
Accession Version	XM_021469751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	OTU domain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGAATGGGA AAGCAGAGGA GGTCGGAGGC CAAACAGAAT GCAGTCTCAG 
TCGGCACCCA GCGTTCGTGT ACATAAGCAA CACTCCTGGC CTCCGCAGGC CACAACTGAT 
GATGCAAGAA ACAAGTTCAG ACGGTCTGAT CAGCGCAGCA GTCCTGTGTG TATTCTGCCA 
GAAGAGGAAG AGGAAAGCGT TGTTTTGGAG CTTCTGCACA AAGATGAGCA CAACTTCCCC 
TCACTTGGAC CCAGCCCCCA AACGGCCACT CCTGGTGAGG TCATAAAGAA AGGAGGGGAA 
AAGAAAAGCC ATCGGAAAAA GGAACAGAAG GAGAACTTCA TGGAGACAGA AGCATCTCAG 
AAGCCTTTAC AGAGGCAGAA GAGTGGGACT GAAGAAAAAC ATGGGATAAA GTTTTCTGAG 
GAACCAAGGC AAAGGTCTTC TTCAACAATA AACGAGAAAT TACAAGAGAA ACCTATCCCT 
GTCGCTGCTT CTCCACCTTC ACCTGCTATC TTCTTATCTG GTCCCAAAAC ACCCATTTCT 
TCATCTAATT CTGCCACTTC CCCTGATCCT AACCGGTCTG CTCCTGAGGC AGTTAAAAAG 
GCAACCATCC AACCTAAGTC TAGCCCTGCT CAAAACCCTG ACAACAGTCA TTTGGAACCA 
GTGTCAACTT TAGCCTCTTT ACAGCACATC TCTAGGGTAG CACCAGTCTC AGAAACCACT 
CCTGCCTCGC AGTTTATCTC TGGTGCAGCA CCAGTGTCAG TTGTCCCACC TGCCTCACAG 
TCTATCTCTG CCACCACTCC TGCCCCACAG TCTATCTCTG CTGCAGCACC AGTTTCTGTT 
TCCACCCCTG CCTCAGACTC TATACTTGTG GCAGCACCAG ACTCAGCTAC CACCCCTGCC 
TCACAGTATA TCCCTGGGTC AAGGGCAGTC TCAGTTAGCA CCCCTGCCCC ACAGTCTATC 
TCTGGGGCAA CAGCAGTCTC ATCTAACACC CCTACCCCAC ACTCCATCTC TAGGGCAACA 
GCAGTCTCAG TTACTGCTCC TGCTCCACAC TTCACCTTTG TGGCAACAGC AGAATCCATT 
TCCACCCCTT CCCCACACTC TATCTCTGGA GCAACAGCAG TTGCCACCCC TGCTCCTATT 
CAATCTGCAC CTGCACTAAA TTCTACCAAA CCCTCATTCC AAATGGCCCA ATTGCCTACA 
GTGGAGCTCC TGCCCAGAAC TGAAGCACCA TCTCAGTTCA CCTCGGTTGA ATTGGTTGCA 
TCTCAGATCA CCCTAGTCCC TTTACCTACT TCTACCCCAA CCCAAACTAC TCTGGCCCCT 
ACTTGCCCAA TCCCAGCTGC CCCAGTTGAA TCCAGCTTGT GCATTCAAAC TTTGCCACCA 
GCCAATACTT CTACTCTATC CCCAACACCA TCATCCACAA TACCGATTTC TGCACAGGCT 
CAGGTTGCTA CCCCTGTATT AGATTCCCCA GGCTTGTCTT CTACCCCACA TCCTATTCAT 
GCAAACAATG CTCCACCAAT TCCCAAAAAG ACTGTTGATC CTCAAACCAC TATGATGCCA 
GTCCCTCTTC CCCATGCTGC TCCCACCTCT ATCTCCCCAT CTATTGGTTC TGCTAATGCT 
TCACCTCCTG ACTTACCTCT AGTCTCTCCA AAAGAATCAG TCCCATCTGC TTGTGAAACA 
TCAACCATAC CTGCCTCTGG TGCAGCGCCT GTTGTTGACC ACCCATCCCA ACCCCAACAC 
CTGCCCTTTC CTCAAGTCCA GTGGTCTCAG CTCCTACAGG ACCCAGTTTA TCCAGGATTT 
CCACAGAATG AGAAGGGACA GTTGGAACCA CCTCCACCGT TTTCCTACTC CCAGAAAGGA 
GAGGACCTAC CGAAAGATAT TAGTGTCCTC AAGTTCTTCT TCAACCTTGG GGTCAAGGCT 
TACACCCAGC CAATGTTTCC TCCTATTGTA TATCTGGCAC CACTGAACCA AGCCTACCTT 
AGAGGTCCTT CTCCTTCCTC TGACTCTCCT GCTAATCCCA CCATAACTTC GTGGCAGCCA 
GAAAACCCTT CTTCTCAGCC TCAAAGTTTG AGCTCCATGG TTGACACCCC ATTGGACAGC 
CATGCTCCAA TTGCACCTGT AGCAGCTGGT AGCAGTCCTG TTGATCCCAT AGAGGTAAGA 
GGATACAACG ATCCTCCATC GATTCCAATG CATGTGCCAC CAAGACCACC AATGCCTTGG 
TCAGCTCCTT CTGTTTCAAG TGCCTATCCT GGGGGTTACC CAACTACTCC TCCAGTGCAG 
TTTTCCACAC CTCCATATCC ACCCCCTGGG AATCAGATGT ATCCACCATC ATCTGTTGGA 
TACCATCGTA TGCCATTTCC ACCAATGCCT TTAGAAGCAA TAAACCATGG GCCTCATGGT 
CCGATTGATG TCTTGCCTTT TGCTCGCCCC ACACTTGAAA GAGGTCAAGA GAATGGTCAG 
TTGTCAATAA ACCCCCAATT TAGGTCTGTA CAGAACTCTG CTGGCTTTCC TGGAGGCCAA 
CCTCATTTTT TGAGTGGTGT AGATTTCAAG TCAGTAGATA CTCCTGTTTC TCTGGGTGGA 
ATGGAACCTC CTCCTTATGG TGGACACTGT TCGGTACCAT CTTTTCAGAT TGGCCCATTG 
CAAGGAGACA TGTCAGGATT GATCCCTTTA CCAAATGGGA GCTTAGGAAG ACAAGGGGGG 
GCCTTCCCGA AGAACCTACA TGCTGAAGAC TCTTCCCGCC TCGTCCATAC ATTTTCTCCT 
GAAGAAAAGT GGCCAGAGGA AATGGAGTCC ACCAATGTAG ACTTGAGAGT TCCTCAGTTA 
TACTACAGCC ATTCCTACAG GGGTGGAGGA AGGCGTGGCC AAGAGGACAG AGGAGGATAT 
CGAGGGCGGA GCCAACGTGG ACGGAAGGAT TATATAGGCC GAGGCAGACA AGACGACCAG 
TCCTACAGAC ATTACGGGAG ACGAGGTGCA GGGCCACAGT TGCCATATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325426.1
CDS	415..4488
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLNPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGIKFSEEPRQRSSSTINEKLQEKPIPVAASPPSPAIFLSGPKTPISSSNSATSPDPNRSAPEAVKKATIQPKSSPAQNPDNSHLEPVSTLASLQHISRVAPVSETTPASQFISGAAPVSVVPPASQSISATTPAPQSISAAAPVSVSTPASDSILVAAPDSATTPASQYIPGSRAVSVSTPAPQSISGATAVSSNTPTPHSISRATAVSVTAPAPHFTFVATAESISTPSPHSISGATAVATPAPIQSAPALNSTKPSFQMAQLPTVELLPRTEAPSQFTSVELVASQITLVPLPTSTPTQTTLAPTCPIPAAPVESSLCIQTLPPANTSTLSPTPSSTIPISAQAQVATPVLDSPGLSSTPHPIHANNAPPIPKKTVDPQTTMMPVPLPHAAPTSISPSIGSANASPPDLPLVSPKESVPSACETSTIPASGAAPVVDHPSQPQHLPFPQVQWSQLLQDPVYPGFPQNEKGQLEPPPPFSYSQKGEDLPKDISVLKFFFNLGVKAYTQPMFPPIVYLAPLNQAYLRGPSPSSDSPANPTITSWQPENPSSQPQSLSSMVDTPLDSHAPIAPVAAGSSPVDPIEVRGYNDPPSIPMHVPPRPPMPWSAPSVSSAYPGGYPTTPPVQFSTPPYPPPGNQMYPPSSVGYHRMPFPPMPLEAINHGPHGPIDVLPFARPTLERGQENGQLSINPQFRSVQNSAGFPGGQPHFLSGVDFKSVDTPVSLGGMEPPPYGGHCSVPSFQIGPLQGDMSGLIPLPNGSLGRQGGAFPKNLHAEDSSRLVHTFSPEEKWPEEMESTNVDLRVPQLYYSHSYRGGGRRGQEDRGGYRGRSQRGRKDYIGRGRQDDQSYRHYGRRGAGPQLPYQ

Target ORF information:

RefSeq Version	XM_021469751.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469751.1

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTTCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAACGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAACCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGAATGGGA AAGCAGAGGA GGTCGGAGGC CAAACAGAAT GCAGTCTCAG 
TCGGCACCCA GCGTTCGTGT ACATAAGCAA CACTCCTGGC CTCCGCAGGC CACAACTGAT 
GATGCAAGAA ACAAGTTCAG ACGGTCTGAT CAGCGCAGCA GTCCTGTGTG TATTCTGCCA 
GAAGAGGAAG AGGAAAGCGT TGTTTTGGAG CTTCTGCACA AAGATGAGCA CAACTTCCCC 
TCACTTGGAC CCAGCCCCCA AACGGCCACT CCTGGTGAGG TCATAAAGAA AGGAGGGGAA 
AAGAAAAGCC ATCGGAAAAA GGAACAGAAG GAGAACTTCA TGGAGACAGA AGCATCTCAG 
AAGCCTTTAC AGAGGCAGAA GAGTGGGACT GAAGAAAAAC ATGGGATAAA GTTTTCTGAG 
GAACCAAGGC AAAGGTCTTC TTCAACAATA AACGAGAAAT TACAAGAGAA ACCTATCCCT 
GTCGCTGCTT CTCCACCTTC ACCTGCTATC TTCTTATCTG GTCCCAAAAC ACCCATTTCT 
TCATCTAATT CTGCCACTTC CCCTGATCCT AACCGGTCTG CTCCTGAGGC AGTTAAAAAG 
GCAACCATCC AACCTAAGTC TAGCCCTGCT CAAAACCCTG ACAACAGTCA TTTGGAACCA 
GTGTCAACTT TAGCCTCTTT ACAGCACATC TCTAGGGTAG CACCAGTCTC AGAAACCACT 
CCTGCCTCGC AGTTTATCTC TGGTGCAGCA CCAGTGTCAG TTGTCCCACC TGCCTCACAG 
TCTATCTCTG CCACCACTCC TGCCCCACAG TCTATCTCTG CTGCAGCACC AGTTTCTGTT 
TCCACCCCTG CCTCAGACTC TATACTTGTG GCAGCACCAG ACTCAGCTAC CACCCCTGCC 
TCACAGTATA TCCCTGGGTC AAGGGCAGTC TCAGTTAGCA CCCCTGCCCC ACAGTCTATC 
TCTGGGGCAA CAGCAGTCTC ATCTAACACC CCTACCCCAC ACTCCATCTC TAGGGCAACA 
GCAGTCTCAG TTACTGCTCC TGCTCCACAC TTCACCTTTG TGGCAACAGC AGAATCCATT 
TCCACCCCTT CCCCACACTC TATCTCTGGA GCAACAGCAG TTGCCACCCC TGCTCCTATT 
CAATCTGCAC CTGCACTAAA TTCTACCAAA CCCTCATTCC AAATGGCCCA ATTGCCTACA 
GTGGAGCTCC TGCCCAGAAC TGAAGCACCA TCTCAGTTCA CCTCGGTTGA ATTGGTTGCA 
TCTCAGATCA CCCTAGTCCC TTTACCTACT TCTACCCCAA CCCAAACTAC TCTGGCCCCT 
ACTTGCCCAA TCCCAGCTGC CCCAGTTGAA TCCAGCTTGT GCATTCAAAC TTTGCCACCA 
GCCAATACTT CTACTCTATC CCCAACACCA TCATCCACAA TACCGATTTC TGCACAGGCT 
CAGGTTGCTA CCCCTGTATT AGATTCCCCA GGCTTGTCTT CTACCCCACA TCCTATTCAT 
GCAAACAATG CTCCACCAAT TCCCAAAAAG ACTGTTGATC CTCAAACCAC TATGATGCCA 
GTCCCTCTTC CCCATGCTGC TCCCACCTCT ATCTCCCCAT CTATTGGTTC TGCTAATGCT 
TCACCTCCTG ACTTACCTCT AGTCTCTCCA AAAGAATCAG TCCCATCTGC TTGTGAAACA 
TCAACCATAC CTGCCTCTGG TGCAGCGCCT GTTGTTGACC ACCCATCCCA ACCCCAACAC 
CTGCCCTTTC CTCAAGTCCA GTGGTCTCAG CTCCTACAGG ACCCAGTTTA TCCAGGATTT 
CCACAGAATG AGAAGGGACA GTTGGAACCA CCTCCACCGT TTTCCTACTC CCAGAAAGGA 
GAGGACCTAC CGAAAGATAT TAGTGTCCTC AAGTTCTTCT TCAACCTTGG GGTCAAGGCT 
TACACCCAGC CAATGTTTCC TCCTATTGTA TATCTGGCAC CACTGAACCA AGCCTACCTT 
AGAGGTCCTT CTCCTTCCTC TGACTCTCCT GCTAATCCCA CCATAACTTC GTGGCAGCCA 
GAAAACCCTT CTTCTCAGCC TCAAAGTTTG AGCTCCATGG TTGACACCCC ATTGGACAGC 
CATGCTCCAA TTGCACCTGT AGCAGCTGGT AGCAGTCCTG TTGATCCCAT AGAGGTAAGA 
GGATACAACG ATCCTCCATC GATTCCAATG CATGTGCCAC CAAGACCACC AATGCCTTGG 
TCAGCTCCTT CTGTTTCAAG TGCCTATCCT GGGGGTTACC CAACTACTCC TCCAGTGCAG 
TTTTCCACAC CTCCATATCC ACCCCCTGGG AATCAGATGT ATCCACCATC ATCTGTTGGA 
TACCATCGTA TGCCATTTCC ACCAATGCCT TTAGAAGCAA TAAACCATGG GCCTCATGGT 
CCGATTGATG TCTTGCCTTT TGCTCGCCCC ACACTTGAAA GAGGTCAAGA GAATGGTCAG 
TTGTCAATAA ACCCCCAATT TAGGTCTGTA CAGAACTCTG CTGGCTTTCC TGGAGGCCAA 
CCTCATTTTT TGAGTGGTGT AGATTTCAAG TCAGTAGATA CTCCTGTTTC TCTGGGTGGA 
ATGGAACCTC CTCCTTATGG TGGACACTGT TCGGTACCAT CTTTTCAGAT TGGCCCATTG 
CAAGGAGACA TGTCAGGATT GATCCCTTTA CCAAATGGGA GCTTAGGAAG ACAAGGGGGG 
GCCTTCCCGA AGAACCTACA TGCTGAAGAC TCTTCCCGCC TCGTCCATAC ATTTTCTCCT 
GAAGAAAAGT GGCCAGAGGA AATGGAGTCC ACCAATGTAG ACTTGAGAGT TCCTCAGTTA 
TACTACAGCC ATTCCTACAG GGGTGGAGGA AGGCGTGGCC AAGAGGACAG AGGAGGATAT 
CGAGGGCGGA GCCAACGTGG ACGGAAGGAT TATATAGGCC GAGGCAGACA AGACGACCAG 
TCCTACAGAC ATTACGGGAG ACGAGGTGCA GGGCCACAGT TGCCATATCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTUD4 ( XM_005174151.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174208.1 Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28118
Clone ID Related Accession (Same CDS sequence)	XM_005174151.3
Accession Version	XM_005174151.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	OTU domain-containing protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGAC GGTCTGATCA GCGCAGCAGT 
CCTGTGTGTA TTCTGCCAGA AGAGGAAGAG GAAAGCGTTG TTTTGGAGCT TCTGCACAAA 
GATGAGCACA ACTTCCCCTC ACTTGGACCC AGCCCCCAAA CGGCCACTCC TGGTGAGGTC 
ATAAAGAAAG GAGGGGAAAA GAAAAGCCAT CGGAAAAAGG AACAGAAGGA GAACTTCATG 
GAGACAGAAG CATCTCAGAA GCCTTTACAG AGGCAGAAGA GTGGGACTGA AGAAAAACAT 
GGGATAAAGT TTTCTGAGGA ACCAAGGCAA AGGTCTTCTT CAACAATAAA CGAGAAATTA 
CAAGAGAAAC CTATCCCTGT CGCTGCTTCT CCACCTTCAC CTGCTATCTT CTTATCTGGT 
CCCAAAACAC CCATTTCTTC ATCTAATTCT GCCACTTCCC CTGATCCTAA CCGGTCTGCT 
CCTGAGGCAG TTAAAAAGGC AACCATCCAA CCTAAGTCTA GCCCTGCTCA AAACCCTGAC 
AACAGTCATT TGGAACCAGT GTCAACTTTA GCCTCTTTAC AGCACATCTC TAGGGTAGCA 
CCAGTCTCAG AAACCACTCC TGCCTCGCAG TTTATCTCTG GTGCAGCACC AGTGTCAGTT 
GTCCCACCTG CCTCACAGTC TATCTCTGCC ACCACTCCTG CCCCACAGTC TATCTCTGCT 
GCAGCACCAG TTTCTGTTTC CACCCCTGCC TCAGACTCTA TACTTGTGGC AGCACCAGAC 
TCAGCTACCA CCCCTGCCTC ACAGTATATC CCTGGGTCAA GGGCAGTCTC AGTTAGCACC 
CCTGCCCCAC AGTCTATCTC TGGGGCAACA GCAGTCTCAT CTAACACCCC TACCCCACAC 
TCCATCTCTA GGGCAACAGC AGTCTCAGTT ACTGCTCCTG CTCCACACTT CACCTTTGTG 
GCAACAGCAG AATCCATTTC CACCCCTTCC CCACACTCTA TCTCTGGAGC AACAGCAGTT 
GCCACCCCTG CTCCTATTCA ATCTGCACCT GCACTAAATT CTACCAAACC CTCATTCCAA 
ATGGCCCAAT TGCCTACAGT GGAGCTCCTG CCCAGAACTG AAGCACCATC TCAGTTCACC 
TCGGTTGAAT TGGTTGCATC TCAGATCACC CTAGTCCCTT TACCTACTTC TACCCCAACC 
CAAACTACTC TGGCCCCTAC TTGCCCAATC CCAGCTGCCC CAGTTGAATC CAGCTTGTGC 
ATTCAAACTT TGCCACCAGC CAATACTTCT ACTCTATCCC CAACACCATC ATCCACAATA 
CCGATTTCTG CACAGGCTCA GGTTGCTACC CCTGTATTAG ATTCCCCAGG CTTGTCTTCT 
ACCCCACATC CTATTCATGC AAACAATGCT CCACCAATTC CCAAAAAGAC TGTTGATCCT 
CAAACCACTA TGATGCCAGT CCCTCTTCCC CATGCTGCTC CCACCTCTAT CTCCCCATCT 
ATTGGTTCTG CTAATGCTTC ACCTCCTGAC TTACCTCTAG TCTCTCCAAA AGAATCAGTC 
CCATCTGCTT GTGAAACATC AACCATACCT GCCTCTGGTG CAGCGCCTGT TGTTGACCAC 
CCATCCCAAC CCCAACACCT GCCCTTTCCT CAAGTCCAGT GGTCTCAGCT CCTACAGGAC 
CCAGTTTATC CAGGATTTCC ACAGAATGAG AAGGGACAGT TGGAACCACC TCCACCGTTT 
TCCTACTCCC AGAAAGGAGA GGACCTACCG AAAGATATTA GTGTCCTCAA GTTCTTCTTC 
AACCTTGGGG TCAAGGCTTA CACCCAGCCA ATGTTTCCTC CTATTGTATA TCTGGCACCA 
CTGAACCAAG CCTACCTTAG AGGTCCTTCT CCTTCCTCTG ACTCTCCTGC TAATCCCACC 
ATAACTTCGT GGCAGCCAGA AAACCCTTCT TCTCAGCCTC AAAGTTTGAG CTCCATGGTT 
GACACCCCAT TGGACAGCCA TGCTCCAATT GCACCTGTAG CAGCTGGTAG CAGTCCTGTT 
GATCCCATAG AGGTAAGAGG ATACAACGAT CCTCCATCGA TTCCAATGCA TGTGCCACCA 
AGACCACCAA TGCCTTGGTC AGCTCCTTCT GTTTCAAGTG CCTATCCTGG GGGTTACCCA 
ACTACTCCTC CAGTGCAGTT TTCCACACCT CCATATCCAC CCCCTGGGAA TCAGATGTAT 
CCACCATCAT CTGTTGGATA CCATCGTATG CCATTTCCAC CAATGCCTTT AGAAGCAATA 
AACCATGGGC CTCATGGTCC GATTGATGTC TTGCCTTTTG CTCGCCCCAC ACTTGAAAGA 
GGTCAAGAGA ATGGTCAGTT GTCAATAAAC CCCCAATTTA GGTCTGTACA GAACTCTGCT 
GGCTTTCCTG GAGGCCAACC TCATTTTTTG AGTGGTGTAG ATTTCAAGTC AGTAGATACT 
CCTGTTTCTC TGGGTGGAAT GGAACCTCCT CCTTATGGTG GACACTGTTC GGTACCATCT 
TTTCAGATTG GCCCATTGCA AGGAGACATG TCAGGATTGA TCCCTTTACC AAATGGGAGC 
TTAGGAAGAC AAGGGGGGGC CTTCCCGAAG AACCTACATG CTGAAGACTC TTCCCGCCTC 
GTCCATACAT TTTCTCCTGA AGAAAAGTGG CCAGAGGAAA TGGAGTCCAC CAATGTAGAC 
TTGAGAGTTC CTCAGTTATA CTACAGCCAT TCCTACAGGG GTGGAGGAAG GCGTGGCCAA 
GAGGACAGAG GAGGATATCG AGGGCGGAGC CAACGTGGAC GGAAGGATTA TATAGGCCGA 
GGCAGACAAG ACGACCAGTC CTACAGACAT TACGGGAGAC GAGGTGCAGG GCCACAGTTG 
CCATATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174208.1
CDS	293..4384
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLSPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNGAQIPSEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGIKFSEEPRQRSSSTINEKLQEKPIPVAASPPSPAIFLSGPKTPISSSNSATSPDPNRSAPEAVKKATIQPKSSPAQNPDNSHLEPVSTLASLQHISRVAPVSETTPASQFISGAAPVSVVPPASQSISATTPAPQSISAAAPVSVSTPASDSILVAAPDSATTPASQYIPGSRAVSVSTPAPQSISGATAVSSNTPTPHSISRATAVSVTAPAPHFTFVATAESISTPSPHSISGATAVATPAPIQSAPALNSTKPSFQMAQLPTVELLPRTEAPSQFTSVELVASQITLVPLPTSTPTQTTLAPTCPIPAAPVESSLCIQTLPPANTSTLSPTPSSTIPISAQAQVATPVLDSPGLSSTPHPIHANNAPPIPKKTVDPQTTMMPVPLPHAAPTSISPSIGSANASPPDLPLVSPKESVPSACETSTIPASGAAPVVDHPSQPQHLPFPQVQWSQLLQDPVYPGFPQNEKGQLEPPPPFSYSQKGEDLPKDISVLKFFFNLGVKAYTQPMFPPIVYLAPLNQAYLRGPSPSSDSPANPTITSWQPENPSSQPQSLSSMVDTPLDSHAPIAPVAAGSSPVDPIEVRGYNDPPSIPMHVPPRPPMPWSAPSVSSAYPGGYPTTPPVQFSTPPYPPPGNQMYPPSSVGYHRMPFPPMPLEAINHGPHGPIDVLPFARPTLERGQENGQLSINPQFRSVQNSAGFPGGQPHFLSGVDFKSVDTPVSLGGMEPPPYGGHCSVPSFQIGPLQGDMSGLIPLPNGSLGRQGGAFPKNLHAEDSSRLVHTFSPEEKWPEEMESTNVDLRVPQLYYSHSYRGGGRRGQEDRGGYRGRSQRGRKDYIGRGRQDDQSYRHYGRRGAGPQLPYQ

Target ORF information:

RefSeq Version	XM_005174151.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174151.3

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGGTGCGCA AATTCCTTCG GAATGGGAAA GCAGAGGAGG TCGGAGGCCA 
AACAGAATGC AGTCTCAGTC GGCACCCAGC GTTCGTGTAC ATAAGCAACA CTCCTGGCCT 
CCGCAGGCCA CAACTGATGA TGCAAGAAAC AAGTTCAGAC GGTCTGATCA GCGCAGCAGT 
CCTGTGTGTA TTCTGCCAGA AGAGGAAGAG GAAAGCGTTG TTTTGGAGCT TCTGCACAAA 
GATGAGCACA ACTTCCCCTC ACTTGGACCC AGCCCCCAAA CGGCCACTCC TGGTGAGGTC 
ATAAAGAAAG GAGGGGAAAA GAAAAGCCAT CGGAAAAAGG AACAGAAGGA GAACTTCATG 
GAGACAGAAG CATCTCAGAA GCCTTTACAG AGGCAGAAGA GTGGGACTGA AGAAAAACAT 
GGGATAAAGT TTTCTGAGGA ACCAAGGCAA AGGTCTTCTT CAACAATAAA CGAGAAATTA 
CAAGAGAAAC CTATCCCTGT CGCTGCTTCT CCACCTTCAC CTGCTATCTT CTTATCTGGT 
CCCAAAACAC CCATTTCTTC ATCTAATTCT GCCACTTCCC CTGATCCTAA CCGGTCTGCT 
CCTGAGGCAG TTAAAAAGGC AACCATCCAA CCTAAGTCTA GCCCTGCTCA AAACCCTGAC 
AACAGTCATT TGGAACCAGT GTCAACTTTA GCCTCTTTAC AGCACATCTC TAGGGTAGCA 
CCAGTCTCAG AAACCACTCC TGCCTCGCAG TTTATCTCTG GTGCAGCACC AGTGTCAGTT 
GTCCCACCTG CCTCACAGTC TATCTCTGCC ACCACTCCTG CCCCACAGTC TATCTCTGCT 
GCAGCACCAG TTTCTGTTTC CACCCCTGCC TCAGACTCTA TACTTGTGGC AGCACCAGAC 
TCAGCTACCA CCCCTGCCTC ACAGTATATC CCTGGGTCAA GGGCAGTCTC AGTTAGCACC 
CCTGCCCCAC AGTCTATCTC TGGGGCAACA GCAGTCTCAT CTAACACCCC TACCCCACAC 
TCCATCTCTA GGGCAACAGC AGTCTCAGTT ACTGCTCCTG CTCCACACTT CACCTTTGTG 
GCAACAGCAG AATCCATTTC CACCCCTTCC CCACACTCTA TCTCTGGAGC AACAGCAGTT 
GCCACCCCTG CTCCTATTCA ATCTGCACCT GCACTAAATT CTACCAAACC CTCATTCCAA 
ATGGCCCAAT TGCCTACAGT GGAGCTCCTG CCCAGAACTG AAGCACCATC TCAGTTCACC 
TCGGTTGAAT TGGTTGCATC TCAGATCACC CTAGTCCCTT TACCTACTTC TACCCCAACC 
CAAACTACTC TGGCCCCTAC TTGCCCAATC CCAGCTGCCC CAGTTGAATC CAGCTTGTGC 
ATTCAAACTT TGCCACCAGC CAATACTTCT ACTCTATCCC CAACACCATC ATCCACAATA 
CCGATTTCTG CACAGGCTCA GGTTGCTACC CCTGTATTAG ATTCCCCAGG CTTGTCTTCT 
ACCCCACATC CTATTCATGC AAACAATGCT CCACCAATTC CCAAAAAGAC TGTTGATCCT 
CAAACCACTA TGATGCCAGT CCCTCTTCCC CATGCTGCTC CCACCTCTAT CTCCCCATCT 
ATTGGTTCTG CTAATGCTTC ACCTCCTGAC TTACCTCTAG TCTCTCCAAA AGAATCAGTC 
CCATCTGCTT GTGAAACATC AACCATACCT GCCTCTGGTG CAGCGCCTGT TGTTGACCAC 
CCATCCCAAC CCCAACACCT GCCCTTTCCT CAAGTCCAGT GGTCTCAGCT CCTACAGGAC 
CCAGTTTATC CAGGATTTCC ACAGAATGAG AAGGGACAGT TGGAACCACC TCCACCGTTT 
TCCTACTCCC AGAAAGGAGA GGACCTACCG AAAGATATTA GTGTCCTCAA GTTCTTCTTC 
AACCTTGGGG TCAAGGCTTA CACCCAGCCA ATGTTTCCTC CTATTGTATA TCTGGCACCA 
CTGAACCAAG CCTACCTTAG AGGTCCTTCT CCTTCCTCTG ACTCTCCTGC TAATCCCACC 
ATAACTTCGT GGCAGCCAGA AAACCCTTCT TCTCAGCCTC AAAGTTTGAG CTCCATGGTT 
GACACCCCAT TGGACAGCCA TGCTCCAATT GCACCTGTAG CAGCTGGTAG CAGTCCTGTT 
GATCCCATAG AGGTAAGAGG ATACAACGAT CCTCCATCGA TTCCAATGCA TGTGCCACCA 
AGACCACCAA TGCCTTGGTC AGCTCCTTCT GTTTCAAGTG CCTATCCTGG GGGTTACCCA 
ACTACTCCTC CAGTGCAGTT TTCCACACCT CCATATCCAC CCCCTGGGAA TCAGATGTAT 
CCACCATCAT CTGTTGGATA CCATCGTATG CCATTTCCAC CAATGCCTTT AGAAGCAATA 
AACCATGGGC CTCATGGTCC GATTGATGTC TTGCCTTTTG CTCGCCCCAC ACTTGAAAGA 
GGTCAAGAGA ATGGTCAGTT GTCAATAAAC CCCCAATTTA GGTCTGTACA GAACTCTGCT 
GGCTTTCCTG GAGGCCAACC TCATTTTTTG AGTGGTGTAG ATTTCAAGTC AGTAGATACT 
CCTGTTTCTC TGGGTGGAAT GGAACCTCCT CCTTATGGTG GACACTGTTC GGTACCATCT 
TTTCAGATTG GCCCATTGCA AGGAGACATG TCAGGATTGA TCCCTTTACC AAATGGGAGC 
TTAGGAAGAC AAGGGGGGGC CTTCCCGAAG AACCTACATG CTGAAGACTC TTCCCGCCTC 
GTCCATACAT TTTCTCCTGA AGAAAAGTGG CCAGAGGAAA TGGAGTCCAC CAATGTAGAC 
TTGAGAGTTC CTCAGTTATA CTACAGCCAT TCCTACAGGG GTGGAGGAAG GCGTGGCCAA 
GAGGACAGAG GAGGATATCG AGGGCGGAGC CAACGTGGAC GGAAGGATTA TATAGGCCGA 
GGCAGACAAG ACGACCAGTC CTACAGACAT TACGGGAGAC GAGGTGCAGG GCCACAGTTG 
CCATATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTUD4 ( XM_001922973.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001923008.4 Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X2, mRNA.

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CloneID	ODa28119
Clone ID Related Accession (Same CDS sequence)	XM_001922973.6
Accession Version	XM_001922973.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	OTU domain-containing protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922973.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGAATGGGA AAGCAGAGGA GGTCGGAGGC CAAACAGAAT GCAGTCTCAG 
TCGGCACCCA GCGTTCGTGT ACATAAGCAA CACTCCTGGC CTCCGCAGGC CACAACTGAT 
GATGCAAGAA ACAAGTTCAG CAGACGGTCT GATCAGCGCA GCAGTCCTGT GTGTATTCTG 
CCAGAAGAGG AAGAGGAAAG CGTTGTTTTG GAGCTTCTGC ACAAAGATGA GCACAACTTC 
CCCTCACTTG GACCCAGCCC CCAAACGGCC ACTCCTGGTG AGGTCATAAA GAAAGGAGGG 
GAAAAGAAAA GCCATCGGAA AAAGGAACAG AAGGAGAACT TCATGGAGAC AGAAGCATCT 
CAGAAGCCTT TACAGAGGCA GAAGAGTGGG ACTGAAGAAA AACATGGGAT AAAGTTTTCT 
GAGGAACCAA GGCAAAGGTC TTCTTCAACA ATAAACGAGA AATTACAAGA GAAACCTATC 
CCTGTCGCTG CTTCTCCACC TTCACCTGCT ATCTTCTTAT CTGGTCCCAA AACACCCATT 
TCTTCATCTA ATTCTGCCAC TTCCCCTGAT CCTAACCGGT CTGCTCCTGA GGCAGTTAAA 
AAGGCAACCA TCCAACCTAA GTCTAGCCCT GCTCAAAACC CTGACAACAG TCATTTGGAA 
CCAGTGTCAA CTTTAGCCTC TTTACAGCAC ATCTCTAGGG TAGCACCAGT CTCAGAAACC 
ACTCCTGCCT CGCAGTTTAT CTCTGGTGCA GCACCAGTGT CAGTTGTCCC ACCTGCCTCA 
CAGTCTATCT CTGCCACCAC TCCTGCCCCA CAGTCTATCT CTGCTGCAGC ACCAGTTTCT 
GTTTCCACCC CTGCCTCAGA CTCTATACTT GTGGCAGCAC CAGACTCAGC TACCACCCCT 
GCCTCACAGT ATATCCCTGG GTCAAGGGCA GTCTCAGTTA GCACCCCTGC CCCACAGTCT 
ATCTCTGGGG CAACAGCAGT CTCATCTAAC ACCCCTACCC CACACTCCAT CTCTAGGGCA 
ACAGCAGTCT CAGTTACTGC TCCTGCTCCA CACTTCACCT TTGTGGCAAC AGCAGAATCC 
ATTTCCACCC CTTCCCCACA CTCTATCTCT GGAGCAACAG CAGTTGCCAC CCCTGCTCCT 
ATTCAATCTG CACCTGCACT AAATTCTACC AAACCCTCAT TCCAAATGGC CCAATTGCCT 
ACAGTGGAGC TCCTGCCCAG AACTGAAGCA CCATCTCAGT TCACCTCGGT TGAATTGGTT 
GCATCTCAGA TCACCCTAGT CCCTTTACCT ACTTCTACCC CAACCCAAAC TACTCTGGCC 
CCTACTTGCC CAATCCCAGC TGCCCCAGTT GAATCCAGCT TGTGCATTCA AACTTTGCCA 
CCAGCCAATA CTTCTACTCT ATCCCCAACA CCATCATCCA CAATACCGAT TTCTGCACAG 
GCTCAGGTTG CTACCCCTGT ATTAGATTCC CCAGGCTTGT CTTCTACCCC ACATCCTATT 
CATGCAAACA ATGCTCCACC AATTCCCAAA AAGACTGTTG ATCCTCAAAC CACTATGATG 
CCAGTCCCTC TTCCCCATGC TGCTCCCACC TCTATCTCCC CATCTATTGG TTCTGCTAAT 
GCTTCACCTC CTGACTTACC TCTAGTCTCT CCAAAAGAAT CAGTCCCATC TGCTTGTGAA 
ACATCAACCA TACCTGCCTC TGGTGCAGCG CCTGTTGTTG ACCACCCATC CCAACCCCAA 
CACCTGCCCT TTCCTCAAGT CCAGTGGTCT CAGCTCCTAC AGGACCCAGT TTATCCAGGA 
TTTCCACAGA ATGAGAAGGG ACAGTTGGAA CCACCTCCAC CGTTTTCCTA CTCCCAGAAA 
GGAGAGGACC TACCGAAAGA TATTAGTGTC CTCAAGTTCT TCTTCAACCT TGGGGTCAAG 
GCTTACACCC AGCCAATGTT TCCTCCTATT GTATATCTGG CACCACTGAA CCAAGCCTAC 
CTTAGAGGTC CTTCTCCTTC CTCTGACTCT CCTGCTAATC CCACCATAAC TTCGTGGCAG 
CCAGAAAACC CTTCTTCTCA GCCTCAAAGT TTGAGCTCCA TGGTTGACAC CCCATTGGAC 
AGCCATGCTC CAATTGCACC TGTAGCAGCT GGTAGCAGTC CTGTTGATCC CATAGAGGTA 
AGAGGATACA ACGATCCTCC ATCGATTCCA ATGCATGTGC CACCAAGACC ACCAATGCCT 
TGGTCAGCTC CTTCTGTTTC AAGTGCCTAT CCTGGGGGTT ACCCAACTAC TCCTCCAGTG 
CAGTTTTCCA CACCTCCATA TCCACCCCCT GGGAATCAGA TGTATCCACC ATCATCTGTT 
GGATACCATC GTATGCCATT TCCACCAATG CCTTTAGAAG CAATAAACCA TGGGCCTCAT 
GGTCCGATTG ATGTCTTGCC TTTTGCTCGC CCCACACTTG AAAGAGGTCA AGAGAATGGT 
CAGTTGTCAA TAAACCCCCA ATTTAGGTCT GTACAGAACT CTGCTGGCTT TCCTGGAGGC 
CAACCTCATT TTTTGAGTGG TGTAGATTTC AAGTCAGTAG ATACTCCTGT TTCTCTGGGT 
GGAATGGAAC CTCCTCCTTA TGGTGGACAC TGTTCGGTAC CATCTTTTCA GATTGGCCCA 
TTGCAAGGAG ACATGTCAGG ATTGATCCCT TTACCAAATG GGAGCTTAGG AAGACAAGGG 
GGGGCCTTCC CGAAGAACCT ACATGCTGAA GACTCTTCCC GCCTCGTCCA TACATTTTCT 
CCTGAAGAAA AGTGGCCAGA GGAAATGGAG TCCACCAATG TAGACTTGAG AGTTCCTCAG 
TTATACTACA GCCATTCCTA CAGGGGTGGA GGAAGGCGTG GCCAAGAGGA CAGAGGAGGA 
TATCGAGGGC GGAGCCAACG TGGACGGAAG GATTATATAG GCCGAGGCAG ACAAGACGAC 
CAGTCCTACA GACATTACGG GAGACGAGGT GCAGGGCCAC AGTTGCCATA TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001923008.4
CDS	294..4370
Translation	MELRAASADAQQQLAGDRTHESRMDEYLRSLGFYRKKIAKDGSCLFRAVAEQILHCQGLHTQVRAACVNYLRQNRPTYELFIEGNFEKYLENLQDPQSWVGQVEISALAELYKHDFIIFQEPDQPPVNLTENGFTHKVRLCFLNGNHYDSVYPESFEKNAAVCQSILYELLYERVCGVERNVVGSCVKGGRGRDRQDSEECKSSEESELEEDDFWSTEPREKTSNGMNARPPQRGRGRGYSRGRGREFLSTKVQKSLSPTLYRNVEYDVWLRSKRLQQQRDFCIAAGMQYSVGDKCKVQLSGSNRLYSAYVKEVSPDNGPVTVFIEELNKQQTVPLLNLRLPSEEPQSWHTVSERGKRQAAPNNEWESRGGRRPNRMQSQSAPSVRVHKQHSWPPQATTDDARNKFSRRSDQRSSPVCILPEEEEESVVLELLHKDEHNFPSLGPSPQTATPGEVIKKGGEKKSHRKKEQKENFMETEASQKPLQRQKSGTEEKHGIKFSEEPRQRSSSTINEKLQEKPIPVAASPPSPAIFLSGPKTPISSSNSATSPDPNRSAPEAVKKATIQPKSSPAQNPDNSHLEPVSTLASLQHISRVAPVSETTPASQFISGAAPVSVVPPASQSISATTPAPQSISAAAPVSVSTPASDSILVAAPDSATTPASQYIPGSRAVSVSTPAPQSISGATAVSSNTPTPHSISRATAVSVTAPAPHFTFVATAESISTPSPHSISGATAVATPAPIQSAPALNSTKPSFQMAQLPTVELLPRTEAPSQFTSVELVASQITLVPLPTSTPTQTTLAPTCPIPAAPVESSLCIQTLPPANTSTLSPTPSSTIPISAQAQVATPVLDSPGLSSTPHPIHANNAPPIPKKTVDPQTTMMPVPLPHAAPTSISPSIGSANASPPDLPLVSPKESVPSACETSTIPASGAAPVVDHPSQPQHLPFPQVQWSQLLQDPVYPGFPQNEKGQLEPPPPFSYSQKGEDLPKDISVLKFFFNLGVKAYTQPMFPPIVYLAPLNQAYLRGPSPSSDSPANPTITSWQPENPSSQPQSLSSMVDTPLDSHAPIAPVAAGSSPVDPIEVRGYNDPPSIPMHVPPRPPMPWSAPSVSSAYPGGYPTTPPVQFSTPPYPPPGNQMYPPSSVGYHRMPFPPMPLEAINHGPHGPIDVLPFARPTLERGQENGQLSINPQFRSVQNSAGFPGGQPHFLSGVDFKSVDTPVSLGGMEPPPYGGHCSVPSFQIGPLQGDMSGLIPLPNGSLGRQGGAFPKNLHAEDSSRLVHTFSPEEKWPEEMESTNVDLRVPQLYYSHSYRGGGRRGQEDRGGYRGRSQRGRKDYIGRGRQDDQSYRHYGRRGAGPQLPYQ

Target ORF information:

RefSeq Version	XM_001922973.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio OTU deubiquitinase 4 (otud4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922973.6

ORF Insert Sequence:

ATGGAGCTCC GGGCGGCTTC AGCGGACGCG CAGCAGCAGC TGGCCGGTGA CAGGACGCAC 
GAGTCCCGCA TGGACGAGTA CCTGCGCTCG CTGGGTTTTT ACCGCAAAAA GATCGCCAAG 
GATGGGTCAT GTCTGTTCAG AGCCGTGGCA GAACAGATAC TGCATTGTCA GGGTTTACAC 
ACACAAGTGC GGGCGGCCTG TGTGAATTAT CTTAGACAGA ACAGACCTAC TTATGAGCTG 
TTTATCGAAG GCAACTTTGA GAAATACCTG GAGAATCTAC AGGATCCTCA GAGCTGGGTT 
GGACAGGTGG AGATCTCTGC ATTGGCTGAG CTCTATAAGC ATGATTTTAT TATTTTCCAG 
GAGCCTGATC AACCGCCTGT TAACCTCACA GAGAACGGCT TCACTCATAA GGTGCGCCTG 
TGCTTCCTCA ACGGAAACCA TTACGACAGT GTTTACCCTG AATCCTTTGA GAAAAATGCT 
GCTGTATGTC AATCGATCCT GTACGAGCTG CTGTATGAGC GAGTGTGTGG CGTCGAACGC 
AACGTTGTGG GATCATGTGT GAAAGGAGGA CGAGGACGAG ATAGACAGGA CTCAGAGGAG 
TGCAAGAGCA GTGAGGAGTC TGAGCTGGAG GAAGACGACT TCTGGTCTAC TGAACCCAGA 
GAGAAGACGA GCAATGGGAT GAATGCCAGA CCACCGCAAA GGGGTCGTGG GAGGGGCTAC 
TCAAGAGGAA GGGGGCGGGA ATTTTTGTCC ACTAAGGTGC AGAAGTCCCT AAGCCCCACC 
CTCTACAGGA ACGTGGAGTA TGACGTGTGG CTCCGTTCGA AACGACTCCA GCAGCAGCGA 
GACTTCTGCA TTGCCGCAGG GATGCAGTAC TCAGTCGGAG ACAAGTGCAA GGTGCAGCTG 
AGTGGCAGCA ATCGGCTCTA TAGTGCTTAT GTTAAAGAAG TCAGTCCAGA CAATGGGCCA 
GTCACTGTCT TTATTGAGGA ACTCAACAAA CAGCAAACGG TGCCGTTGTT AAACTTGCGT 
CTTCCGTCTG AAGAGCCTCA GTCTTGGCAC ACGGTGTCAG AGAGGGGAAA AAGACAAGCT 
GCGCCAAACA ATGAATGGGA AAGCAGAGGA GGTCGGAGGC CAAACAGAAT GCAGTCTCAG 
TCGGCACCCA GCGTTCGTGT ACATAAGCAA CACTCCTGGC CTCCGCAGGC CACAACTGAT 
GATGCAAGAA ACAAGTTCAG CAGACGGTCT GATCAGCGCA GCAGTCCTGT GTGTATTCTG 
CCAGAAGAGG AAGAGGAAAG CGTTGTTTTG GAGCTTCTGC ACAAAGATGA GCACAACTTC 
CCCTCACTTG GACCCAGCCC CCAAACGGCC ACTCCTGGTG AGGTCATAAA GAAAGGAGGG 
GAAAAGAAAA GCCATCGGAA AAAGGAACAG AAGGAGAACT TCATGGAGAC AGAAGCATCT 
CAGAAGCCTT TACAGAGGCA GAAGAGTGGG ACTGAAGAAA AACATGGGAT AAAGTTTTCT 
GAGGAACCAA GGCAAAGGTC TTCTTCAACA ATAAACGAGA AATTACAAGA GAAACCTATC 
CCTGTCGCTG CTTCTCCACC TTCACCTGCT ATCTTCTTAT CTGGTCCCAA AACACCCATT 
TCTTCATCTA ATTCTGCCAC TTCCCCTGAT CCTAACCGGT CTGCTCCTGA GGCAGTTAAA 
AAGGCAACCA TCCAACCTAA GTCTAGCCCT GCTCAAAACC CTGACAACAG TCATTTGGAA 
CCAGTGTCAA CTTTAGCCTC TTTACAGCAC ATCTCTAGGG TAGCACCAGT CTCAGAAACC 
ACTCCTGCCT CGCAGTTTAT CTCTGGTGCA GCACCAGTGT CAGTTGTCCC ACCTGCCTCA 
CAGTCTATCT CTGCCACCAC TCCTGCCCCA CAGTCTATCT CTGCTGCAGC ACCAGTTTCT 
GTTTCCACCC CTGCCTCAGA CTCTATACTT GTGGCAGCAC CAGACTCAGC TACCACCCCT 
GCCTCACAGT ATATCCCTGG GTCAAGGGCA GTCTCAGTTA GCACCCCTGC CCCACAGTCT 
ATCTCTGGGG CAACAGCAGT CTCATCTAAC ACCCCTACCC CACACTCCAT CTCTAGGGCA 
ACAGCAGTCT CAGTTACTGC TCCTGCTCCA CACTTCACCT TTGTGGCAAC AGCAGAATCC 
ATTTCCACCC CTTCCCCACA CTCTATCTCT GGAGCAACAG CAGTTGCCAC CCCTGCTCCT 
ATTCAATCTG CACCTGCACT AAATTCTACC AAACCCTCAT TCCAAATGGC CCAATTGCCT 
ACAGTGGAGC TCCTGCCCAG AACTGAAGCA CCATCTCAGT TCACCTCGGT TGAATTGGTT 
GCATCTCAGA TCACCCTAGT CCCTTTACCT ACTTCTACCC CAACCCAAAC TACTCTGGCC 
CCTACTTGCC CAATCCCAGC TGCCCCAGTT GAATCCAGCT TGTGCATTCA AACTTTGCCA 
CCAGCCAATA CTTCTACTCT ATCCCCAACA CCATCATCCA CAATACCGAT TTCTGCACAG 
GCTCAGGTTG CTACCCCTGT ATTAGATTCC CCAGGCTTGT CTTCTACCCC ACATCCTATT 
CATGCAAACA ATGCTCCACC AATTCCCAAA AAGACTGTTG ATCCTCAAAC CACTATGATG 
CCAGTCCCTC TTCCCCATGC TGCTCCCACC TCTATCTCCC CATCTATTGG TTCTGCTAAT 
GCTTCACCTC CTGACTTACC TCTAGTCTCT CCAAAAGAAT CAGTCCCATC TGCTTGTGAA 
ACATCAACCA TACCTGCCTC TGGTGCAGCG CCTGTTGTTG ACCACCCATC CCAACCCCAA 
CACCTGCCCT TTCCTCAAGT CCAGTGGTCT CAGCTCCTAC AGGACCCAGT TTATCCAGGA 
TTTCCACAGA ATGAGAAGGG ACAGTTGGAA CCACCTCCAC CGTTTTCCTA CTCCCAGAAA 
GGAGAGGACC TACCGAAAGA TATTAGTGTC CTCAAGTTCT TCTTCAACCT TGGGGTCAAG 
GCTTACACCC AGCCAATGTT TCCTCCTATT GTATATCTGG CACCACTGAA CCAAGCCTAC 
CTTAGAGGTC CTTCTCCTTC CTCTGACTCT CCTGCTAATC CCACCATAAC TTCGTGGCAG 
CCAGAAAACC CTTCTTCTCA GCCTCAAAGT TTGAGCTCCA TGGTTGACAC CCCATTGGAC 
AGCCATGCTC CAATTGCACC TGTAGCAGCT GGTAGCAGTC CTGTTGATCC CATAGAGGTA 
AGAGGATACA ACGATCCTCC ATCGATTCCA ATGCATGTGC CACCAAGACC ACCAATGCCT 
TGGTCAGCTC CTTCTGTTTC AAGTGCCTAT CCTGGGGGTT ACCCAACTAC TCCTCCAGTG 
CAGTTTTCCA CACCTCCATA TCCACCCCCT GGGAATCAGA TGTATCCACC ATCATCTGTT 
GGATACCATC GTATGCCATT TCCACCAATG CCTTTAGAAG CAATAAACCA TGGGCCTCAT 
GGTCCGATTG ATGTCTTGCC TTTTGCTCGC CCCACACTTG AAAGAGGTCA AGAGAATGGT 
CAGTTGTCAA TAAACCCCCA ATTTAGGTCT GTACAGAACT CTGCTGGCTT TCCTGGAGGC 
CAACCTCATT TTTTGAGTGG TGTAGATTTC AAGTCAGTAG ATACTCCTGT TTCTCTGGGT 
GGAATGGAAC CTCCTCCTTA TGGTGGACAC TGTTCGGTAC CATCTTTTCA GATTGGCCCA 
TTGCAAGGAG ACATGTCAGG ATTGATCCCT TTACCAAATG GGAGCTTAGG AAGACAAGGG 
GGGGCCTTCC CGAAGAACCT ACATGCTGAA GACTCTTCCC GCCTCGTCCA TACATTTTCT 
CCTGAAGAAA AGTGGCCAGA GGAAATGGAG TCCACCAATG TAGACTTGAG AGTTCCTCAG 
TTATACTACA GCCATTCCTA CAGGGGTGGA GGAAGGCGTG GCCAAGAGGA CAGAGGAGGA 
TATCGAGGGC GGAGCCAACG TGGACGGAAG GATTATATAG GCCGAGGCAG ACAAGACGAC 
CAGTCCTACA GACATTACGG GAGACGAGGT GCAGGGCCAC AGTTGCCATA TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish transforming growth factor-?-stimulated clone 22 domain 3 (TSC22D3) plays critical roles in Bmp-dependent dorsoventral patterning via two deubiquitylating enzymes Usp15 and Otud4.
Biochimica et biophysica acta1830(10)4584-93(2013 Oct)
Tse WK,Jiang YJ,Wong CK

Ataxia, dementia, and hypogonadotropism caused by disordered ubiquitination.
The New England journal of medicine368(21)1992-2003(2013 May)
Margolin DH,Kousi M,Chan YM,Lim ET,Schmahmann JD,Hadjivassiliou M,Hall JE,Adam I,Dwyer A,Plummer L,Aldrin SV,O'Rourke J,Kirby A,Lage K,Milunsky A,Milunsky JM,Chan J,Hedley-Whyte ET,Daly MJ,Katsanis N,Seminara SB

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ

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OTUD4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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