chs1 cDNA ORF clone, Danio rerio(zebrafish)

The following chs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa18334 NM_001316326.1
Latest version!
Danio rerio chitin synthase 1 (chs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa39715 XM_009307025.3
Latest version!
Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa39715 XM_009307025.2 Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa18334
    Clone ID Related Accession (Same CDS sequence) NM_001316326.1
    Accession Version NM_001316326.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC322468
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694274.5. On Oct 16, 2015 this sequence version replaced XM_001920979.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01000281.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
    CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC
    CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC
    AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG
    GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG
    TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA
    CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC
    ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC
    TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC
    CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC
    GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT
    ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG
    TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA
    TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG
    TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA
    GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG
    GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT
    GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG
    ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC
    ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG
    CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC
    GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC
    TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG
    TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA
    ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT
    GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG
    ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC
    TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG
    ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG
    GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA
    CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG
    ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA
    GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT
    CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG
    AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC
    CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT
    TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT
    GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT
    CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA
    TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC
    TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC
    GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT
    CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG
    AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC
    CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG
    TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT
    TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC
    TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC
    ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG
    CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC
    AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC
    ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA
    GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC
    CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG
    CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA
    CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG
    GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCA GCTCCAGCAG CTCTGAGAAG
    AAGATGGACA CGTTCAATGA CGACACAGAC TATGAGGACA CAGACTACGA TGATGATGAC
    GACGACGAGG ATTATGATTA TAATACTGTG GAGCCCAAAG AAGTTGTGCC TGAGAGCGCC
    TGGGTGGAGC CCGTGAAGAG GGAGTTCCTG AAGAAGCTGA CCTACGCCAA TCTGAAGAGG
    AACCTTCAGG AGCAGATCCG CTACACGCTG AGGAACAAGA ACCAGGAGGA CCTGTGCGAG
    GAGCTGGTGC TGATCCTGAC CGACACGCTG AACGACGAGC TGAAGGATAA AGTGGGTCCT
    GAAGACGTCT TGACCGAGAC GCAGCTGGAG GAACTGCAGT ATGCGCTCAA TGAAGCGGCG
    CGGCGCATCG TCAAGACCAA CCGCATCGAG ACCGGCTCAC AGCGGGTGGA GCGGCGGGTC
    AAACGGGGCA TCGAGAGGAC GCTGGTGGCG CCGCAGGTGG AGAAACTGTC AGAGGACGAG
    CAGGATTTCT GGGAGAAGCT GTTGGAGCGC TACCTGAAGC CCATTCAGGA TGATAAAGCT
    CATCTGGAGG AGGTGACGCG GGAGCTCAAG TCTCTGAGGA ACAAAGCCGT GTTTCTGTAC
    TTCATCATTA ACGTCCTCTG GGTCGTAGCC ACGTTCTTCC TGCAGGCCAT CGGCGGAGAC
    GTTCTGAGTA TAGAAATCCC CAAATATTAC CCCAATAAAA CCCTGGCCCC GGAGCCCCTG
    AGGGTGGAGC CGCTCAGCCT GATGTTCCTG CTGTCCTTCG CCGTCCTGCT GGTCGTGCAG
    TTCCTGGCTA TGCTGTACCA CAGGGTCTAC ACGCTCATCC ATGTGGTGTC GTACAGAAGC
    TCAGAGAAGA ACTATAAAGA GAAGGATGAG GAGGATGAGG AAGATGCGCT GATCTTGGGG
    AACATCATCC CCAGCAGTCT GGTGATCACC GGAGACGACC TGTTGAACGG AGACTCCTTC
    ATTCCGAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001303255.1
    CDS59..4450
    Translation

    Target ORF information:

    RefSeq Version NM_001316326.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio chitin synthase 1 (chs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001316326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
    CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC
    CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC
    AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG
    GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG
    TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA
    CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC
    ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC
    TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC
    CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC
    GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT
    ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG
    TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA
    TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG
    TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA
    GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG
    GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT
    GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG
    ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC
    ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG
    CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC
    GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC
    TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG
    TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA
    ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT
    GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG
    ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC
    TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG
    ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG
    GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA
    CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG
    ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA
    GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT
    CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG
    AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC
    CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT
    TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT
    GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT
    CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA
    TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC
    TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC
    GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT
    CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG
    AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC
    CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG
    TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT
    TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC
    TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC
    ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG
    CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC
    AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC
    ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA
    GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC
    CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG
    CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA
    CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG
    GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCA GCTCCAGCAG CTCTGAGAAG
    AAGATGGACA CGTTCAATGA CGACACAGAC TATGAGGACA CAGACTACGA TGATGATGAC
    GACGACGAGG ATTATGATTA TAATACTGTG GAGCCCAAAG AAGTTGTGCC TGAGAGCGCC
    TGGGTGGAGC CCGTGAAGAG GGAGTTCCTG AAGAAGCTGA CCTACGCCAA TCTGAAGAGG
    AACCTTCAGG AGCAGATCCG CTACACGCTG AGGAACAAGA ACCAGGAGGA CCTGTGCGAG
    GAGCTGGTGC TGATCCTGAC CGACACGCTG AACGACGAGC TGAAGGATAA AGTGGGTCCT
    GAAGACGTCT TGACCGAGAC GCAGCTGGAG GAACTGCAGT ATGCGCTCAA TGAAGCGGCG
    CGGCGCATCG TCAAGACCAA CCGCATCGAG ACCGGCTCAC AGCGGGTGGA GCGGCGGGTC
    AAACGGGGCA TCGAGAGGAC GCTGGTGGCG CCGCAGGTGG AGAAACTGTC AGAGGACGAG
    CAGGATTTCT GGGAGAAGCT GTTGGAGCGC TACCTGAAGC CCATTCAGGA TGATAAAGCT
    CATCTGGAGG AGGTGACGCG GGAGCTCAAG TCTCTGAGGA ACAAAGCCGT GTTTCTGTAC
    TTCATCATTA ACGTCCTCTG GGTCGTAGCC ACGTTCTTCC TGCAGGCCAT CGGCGGAGAC
    GTTCTGAGTA TAGAAATCCC CAAATATTAC CCCAATAAAA CCCTGGCCCC GGAGCCCCTG
    AGGGTGGAGC CGCTCAGCCT GATGTTCCTG CTGTCCTTCG CCGTCCTGCT GGTCGTGCAG
    TTCCTGGCTA TGCTGTACCA CAGGGTCTAC ACGCTCATCC ATGTGGTGTC GTACAGAAGC
    TCAGAGAAGA ACTATAAAGA GAAGGATGAG GAGGATGAGG AAGATGCGCT GATCTTGGGG
    AACATCATCC CCAGCAGTCT GGTGATCACC GGAGACGACC TGTTGAACGG AGACTCCTTC
    ATTCCGAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39715
    Clone ID Related Accession (Same CDS sequence) XM_009307025.2 , XM_009307025.3
    Accession Version XM_009307025.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC322468 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
    CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC
    CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC
    AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG
    GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG
    TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA
    CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC
    ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC
    TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC
    CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC
    GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT
    ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG
    TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA
    TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG
    TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA
    GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG
    GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT
    GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG
    ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC
    ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG
    CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC
    GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC
    TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG
    TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA
    ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT
    GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG
    ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC
    TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG
    ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG
    GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA
    CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG
    ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA
    GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT
    CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG
    AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC
    CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT
    TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT
    GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT
    CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA
    TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC
    TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC
    GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT
    CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG
    AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC
    CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG
    TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT
    TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC
    TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC
    ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG
    CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC
    AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC
    ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA
    GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC
    CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG
    CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA
    CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG
    GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG
    ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC
    GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG
    GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC
    CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG
    CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA
    GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG
    CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA
    CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG
    GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT
    CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC
    ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT
    CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG
    GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC
    CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA
    GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC
    ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT
    CCGAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305300.1
    CDS11..4399
    Translation

    Target ORF information:

    RefSeq Version XM_009307025.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307025.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
    CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC
    CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC
    AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG
    GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG
    TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA
    CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC
    ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC
    TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC
    CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC
    GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT
    ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG
    TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA
    TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG
    TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA
    GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG
    GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT
    GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG
    ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC
    ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG
    CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC
    GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC
    TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG
    TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA
    ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT
    GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG
    ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC
    TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG
    ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG
    GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA
    CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG
    ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA
    GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT
    CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG
    AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC
    CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT
    TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT
    GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT
    CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA
    TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC
    TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC
    GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT
    CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG
    AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC
    CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG
    TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT
    TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC
    TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC
    ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG
    CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC
    AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC
    ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA
    GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC
    CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG
    CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA
    CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG
    GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG
    ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC
    GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG
    GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC
    CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG
    CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA
    GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG
    CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA
    CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG
    GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT
    CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC
    ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT
    CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG
    GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC
    CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA
    GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC
    ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT
    CCGAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39715
    Clone ID Related Accession (Same CDS sequence) XM_009307025.2 , XM_009307025.3
    Accession Version XM_009307025.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC322468 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
    CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC
    CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC
    AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG
    GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG
    TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA
    CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC
    ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC
    TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC
    CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC
    GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT
    ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG
    TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA
    TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG
    TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA
    GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG
    GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT
    GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG
    ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC
    ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG
    CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC
    GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC
    TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG
    TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA
    ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT
    GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG
    ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC
    TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG
    ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG
    GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA
    CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG
    ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA
    GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT
    CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG
    AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC
    CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT
    TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT
    GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT
    CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA
    TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC
    TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC
    GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT
    CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG
    AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC
    CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG
    TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT
    TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC
    TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC
    ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG
    CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC
    AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC
    ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA
    GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC
    CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG
    CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA
    CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG
    GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG
    ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC
    GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG
    GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC
    CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG
    CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA
    GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG
    CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA
    CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG
    GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT
    CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC
    ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT
    CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG
    GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC
    CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA
    GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC
    ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT
    CCGAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305300.1
    CDS35..4423
    Translation

    Target ORF information:

    RefSeq Version XM_009307025.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307025.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
    CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC
    CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC
    AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG
    GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG
    TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA
    CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC
    ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC
    TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC
    CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC
    GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT
    ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG
    TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA
    TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG
    TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA
    GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG
    GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT
    GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG
    ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC
    ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG
    CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC
    GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC
    TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG
    TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA
    ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT
    GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG
    ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC
    TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG
    ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG
    GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA
    CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG
    ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA
    GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT
    CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG
    AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC
    CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT
    TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT
    GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT
    CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA
    TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC
    TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC
    GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT
    CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG
    AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC
    CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG
    TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT
    TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC
    TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC
    ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG
    CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC
    AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC
    ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA
    GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC
    CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG
    CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA
    CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG
    GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG
    ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC
    GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG
    GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC
    CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG
    CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA
    GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG
    CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA
    CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG
    GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT
    CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC
    ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT
    CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG
    GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC
    CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA
    GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC
    ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT
    CCGAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.