Chs2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chs2
Entrez Gene ID	40442
Full Name	Chitin synthase 2
Synonyms	CG7464,ChSA,DmCS-2,DmeChSA,Dmel\CG7464
General protein information	Preferred Names Chitin synthase 2 Names CG7464 gene product from transcript CG7464-RD CG7464-PC CG7464-PD CS-2-PC CS-2-PD chitin Syn2 chitin synthase 2 chitin synthase A chitin-synthase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 79A5-79A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079485.4	NP_524209.3	chitin synthase 2, isoform D
NM_001144525.2	NP_001137997.2	chitin synthase 2, isoform C

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:zfos-1762d12.1	XP_001921014.3
Drosophila melanogaster (fruit fly)	CS-2	NP_524209.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001205	XP_321951.2
Danio rerio (zebrafish)	si:ch211-219a15.4	XP_691594.4
Danio rerio (zebrafish)	si:ch211-264e16.2	XP_691803.4
Danio rerio (zebrafish)	LOC101886093	XP_005170020.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19247	NM_001144525.2 Latest version!	Drosophila melanogaster chitin synthase 2, transcript variant C (Chs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa21236	NM_079485.4 Latest version!	Drosophila melanogaster chitin synthase 2, transcript variant D (Chs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chs2 ( NM_001144525.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137997.2 Drosophila melanogaster chitin synthase 2, transcript variant C (Chs2), mRNA.

Price & Availability↑

CloneID	OFa19247
Clone ID Related Accession (Same CDS sequence)	NM_001144525.2
Accession Version	NM_001144525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitin synthase 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001144525.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGAG GAGACTACGA TAGCGGTGAC TACTTCATGC GCAGTCGGAA GCAGCGGGAC 
AAGCCGTCAT TATGGGACTC CTTCCAGGAC CCGCCGTCTA AGAAGACGAG CGGATCCGAT 
GCCGACTGGA AGAAGCTCCA GTTCTGCCTT AAGCTCTTCA AGATCTGCAC CTACCTCCTT 
GTCTTCGCGG TCGTTCTTGC GACGAGCGTG TTCGCCAACT TGATTTATCT CTACATGGCC 
GGAAACACAG GTGGCACCGG GCCACGCGTG CAAGTGTGCA GCAAGTATCT ACTCCGGCAC 
GGCCAAGTGG AGGCGGTGGC CAGCGAGCTG GACCAAATCG CCTGGGTCTG GGCCCTGATC 
TTCGCCTTTG CCGCCCCCGA GCTGCTCACC TTCCTGCGCG CCCTGCGCAT CTGTATGTTC 
AAGCGCGAGC AGCGACCCAG CGGACTAGAA GTGGGACTGG TCACGCTCTT TGAACTCCTG 
CACGTCGTTG GTCTGGCCCT GCTCACCTTC ACCGTGCTGC CGGCCCTGGA TGTGACGCGT 
GGAGCGATTC TGGGCTGCTG CGTCTGCTTC GTGCCTGCCC TGCTTAACCT GCTGACGGGA 
ACGCGGGTGC GCTGGAGGTC GGCGGACAGC CTGGTCCTCG GGCTCTCCAT CCTGGCCCTT 
GTGGCGCAGG GCAGCGTCTT TCTGGTCTGG CCCTCGCTGA GCGACAGTAT GGAGGTCCAG 
CTGCTGGCCA TTCCGCTGCT CCTTATTTCG TTTCGCTGGT GGGAGAACTT CGCCAACACT 
CACGAGGCCA TCGCCTATAT CATTCGCACC ATCCGCTGTA CCCGATCCCG ATACCACACC 
TACCTGTACC TGGCGCCTCT GAAGGTGTTG GTTTTCGCGG GGATGGGCTT CATCCTGCAT 
GGCCACGAAT TCGTTGAGTA CTTTGGTAGG TTCCGGGATG CCTGGCGGCC GCATCTCATC 
ACGCTGGTGC AATCTAATGG CGACCCCGAT GCGCTGCTTT TAGCACAGGC CAACGTATCA 
ATCACAGCCC CTCCACGCAG GACGCTGTTC ACCATGCAGT CTAGTCCGAA TTCGGTTTTC 
TACGCACTGG CGGCCCAGGT GGGTGCTGCG TACCTGTGCC ACATTTTCGG TAAGTTCGCC 
TGCAAAATCA AGATCCAGAA GTTCAGCTAC GCGCTGCCCC TCAGCCTGGC AGGACCTGCC 
ACCGTCTGCC TGGTCACCTT TCTGGCCCAG CTGCGGGCCT CGGACCCGTG CTCCCTGCAC 
GGTTTCATGC CGGACTACCT CTCGCTCCTG GCGCTGGGCG GGAGTGTGGA GGAGCTGGGC 
CAGAGATGCC TCGACTACGC CCTCTGGCTG TGGCCCCTGT GGTGGCTGGC CCAGGTGTGG 
ACCTGCCTGC ACATTTGGCA GCCCCACAAT GACAGGAACG CCCCAACCGA GAAGCTCTTC 
GTGTGTCCAT GGTACTGCGG CCTGCTGGTG GACCAGTGCT CGATGATGAA CAGGCGGATC 
GTGGACTGGA GCGAGGAGTA TTTGGCCATC AAGAGCGACC TAACCGCGCC GAGGGATCCT 
GTGGTGGCTC TGGCAGCCGA CATCAATGCC AGCCGGGACA TCCAGGAGGA GGATAAGGTG 
CCGCAGCTGA TTGTGTGCGC CACCATGTGG CACGAGACCG AGGACGAGAT GATGGAGTTC 
CTCAAGTCAA TTGTCCGCTT GGATGAGGAT CAATGCGCCC GCCGCATGGC CAGGACGCAC 
CTCAACGGCG GGAAGGCGGA CGACGAGTAC TATGAATTGG AAACTAACAT CTTCTTCGAC 
GATGCATTCG TTTCGGATCC GCGTCAGTGC CAGAACAAGC GTAACCCACC CATCAACGAG 
TATGTGAAGA CCCTAACCCG AACCATCGAC AAGGCAGCCT TTGAGGTCTA CGGAGTGAAC 
ATCCGCATAA AGCCCCCTCT TAAGATCGAG ACTCCCTACG GAGGCCGCTT GGTGTGGACC 
CTGCCAGGAC GCACTAAAAT GGTGGCCCAT CTGAAGAACA AGGACAAGAT CCGGCACAAG 
AAGCGCTGGT CGCAGGTCAT GTACATGTAC TATCTTCTGG GATATCGGAT CATGGAGACG 
AAGGAGCTGT CACCCCGTCG AAAGGCGGTT ATTGCAGAGA ACACCTTTAT CTTGGCCCTG 
GACGGAGACA TAGACTTCCA GCCGCGAGCT GTGAGGCTGC TGATCGATCG AATGAAGGCC 
GTGGACGAGC TGGGAGCTGC CTGCGGTCGA ATTCACCCGG TGGGCAGGGG TCCGATGGTG 
TGGTACCAGA GATTTGAGTA CGCCATTGGA CACTGGCTGC AAAAGGCGAC GGAGCATGTC 
ATTGGTTGCG TCCTCTGCAG TCCGGGATGC TTCAGTCTGT TCCGGGCCAG CGCCCTGATG 
GAGAACAGCG TGATGAAGCG GTACACGATG ATCAGTTCGG AGGCGATGAA GATGGTGCAG 
TACGACCAGG GCGAGGACCG CTGGCTGTGT ACGCTGCTCT TGAAGGCCGG CTCCCGGGTG 
GAGTACTGCG CCGCCTCGGA TGCGTATACC CACGCGCCAG AGAGCTTTAA CGAGTTCTAC 
AACCAACGAC GTCGTTGGGT GCCCTCCACT ATAGCCAACA TCTTCGATAT CCTAGCGGAT 
GCGGACACCG TGGTGAAGAA GAACAACTCC ATATCAACGA TGTACGTGTG GTACCAGGGA 
ATGCTGATGA TCGGAACCGT GTTGGGACCG GGCACCATAT TCCTCATGAT GGTGGGCGCC 
TTGGTGGCCG TGTTTTCAAT TGACATCTGG ACCGCTTTCC TATGGAATTT TTTTCCCATC 
CTCTTATTCA TTCTGGCCTG TGTCTACCTT AAACAGAAGT TTCAACTGCT GATAGCATTC 
GTCATTAGCT CCGTTTACTG CCTAGTCATG ATGGCAGTCC TCATCGGCAT CATTGTCCAG 
ATGCTGGAGG ACGGACCCCT GGCTCCTGCA TCGCTGTTCT TCCTGCTCGT AGCTGTGCAA 
ATCACAATTG CAGGCTTTAT GCATCCACAG GAGTTCTGGG CATTGCTGTG CGGAGTCATC 
TACTACATAA CCATCCCTTC GATGTACATG CTTCTCATGA TCTTTTCTGT GTTCAACATG 
AACGATGTTT CGTGGGGCAC TCGAGAGGCT CCAGTGCTCA AGGACGACGG CAAGGACGCA 
GTCGATGAAG CGAACAACTA CCTGCCAGGC TGGCTAAATG ATCCGCTATT GATCGACTCG 
GAGCTGGGCG AAGTGTCGTT GATGGAGCAG AGATTCTGGA AGGATCTGAT AAAGCAGTAT 
CTGAAGCCAT TGGAACTGAG TAGGGAGCAG AAGCAGGCGA TGGCCGACGG ACTTAAGGAG 
CTGAGAAACA TGATCGCCTT CGCCTTTGTG ATGGTCAATG CGATCTTTGT GCTCATTGTG 
TTCCTGCTGC AGCTGAAGAA GGAGTTTCTG CACCTCGAGT GGCCTATTGA TCCTACAGAC 
TTTGTATCCT TCGACAGGGA CAACCTAATG GTTGGCATCT ACAGGCAGTA CAAAGAACTG 
GATCCCATTG GCCTGTGCTT TGTGATTTTC TTTGGACTGA TCCTGATCAT CCAGTTCCTG 
GCGATGTTCT TCCACCGCTT CGCTACCCTG TGCCAGCTGC TGGCCACTAC TCAGGTGGAC 
TGGTTTCGAA CAGGAGGTCA GTTTACTGAC GAGGATGCCG CCACCGAGCT GAAGGGCAGT 
GCTGTCAGTA TAGCCCGGAA GTTGCAGCGA CCCAAGAGGC TCTTCGACGA CGACGAGGAC 
GGGGACCCCC ACTACGACGA GGTGTTGATG GATTCTTTAG AAGGGGAGCA CAGGGAGAGC 
ATGGTGCGAC GGCAGACGAT CTTTCGGCTT CACGAGACGC GGGACAAGCA GCACAGCGAC 
TACAGCAATC TGGTGTTTAA CTTTGAACGC CGTTTCTTTG GAGACGACGA GCTGAACCTT 
AAGAATCTGG CACTCAACCG AAAGTCAGTG AAGCTTCTGC AGGAGCGTCG CTCTGCGGCC 
AAGGTCAATG CAGCAGCCAC GCCCGAGGGC ACGCCAACTC CGAAGGCTGG AAAGCGACCG 
CCTGTGGTGC CGATGGCCAA GAAGGTGAGC TTTACGGCCT CAAACCGGAA CAATATGGCT 
TTGTACGACA ACGGCGGGTA CGAGCACACC GAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137997.2
CDS	31..4209
Translation	MSGGDYDSGDYFMRSRKQRDKPSLWDSFQDPPSKKTSGSDADWKKLQFCLKLFKICTYLLVFAVVLATSVFANLIYLYMAGNTGGTGPRVQVCSKYLLRHGQVEAVASELDQIAWVWALIFAFAAPELLTFLRALRICMFKREQRPSGLEVGLVTLFELLHVVGLALLTFTVLPALDVTRGAILGCCVCFVPALLNLLTGTRVRWRSADSLVLGLSILALVAQGSVFLVWPSLSDSMEVQLLAIPLLLISFRWWENFANTHEAIAYIIRTIRCTRSRYHTYLYLAPLKVLVFAGMGFILHGHEFVEYFGRFRDAWRPHLITLVQSNGDPDALLLAQANVSITAPPRRTLFTMQSSPNSVFYALAAQVGAAYLCHIFGKFACKIKIQKFSYALPLSLAGPATVCLVTFLAQLRASDPCSLHGFMPDYLSLLALGGSVEELGQRCLDYALWLWPLWWLAQVWTCLHIWQPHNDRNAPTEKLFVCPWYCGLLVDQCSMMNRRIVDWSEEYLAIKSDLTAPRDPVVALAADINASRDIQEEDKVPQLIVCATMWHETEDEMMEFLKSIVRLDEDQCARRMARTHLNGGKADDEYYELETNIFFDDAFVSDPRQCQNKRNPPINEYVKTLTRTIDKAAFEVYGVNIRIKPPLKIETPYGGRLVWTLPGRTKMVAHLKNKDKIRHKKRWSQVMYMYYLLGYRIMETKELSPRRKAVIAENTFILALDGDIDFQPRAVRLLIDRMKAVDELGAACGRIHPVGRGPMVWYQRFEYAIGHWLQKATEHVIGCVLCSPGCFSLFRASALMENSVMKRYTMISSEAMKMVQYDQGEDRWLCTLLLKAGSRVEYCAASDAYTHAPESFNEFYNQRRRWVPSTIANIFDILADADTVVKKNNSISTMYVWYQGMLMIGTVLGPGTIFLMMVGALVAVFSIDIWTAFLWNFFPILLFILACVYLKQKFQLLIAFVISSVYCLVMMAVLIGIIVQMLEDGPLAPASLFFLLVAVQITIAGFMHPQEFWALLCGVIYYITIPSMYMLLMIFSVFNMNDVSWGTREAPVLKDDGKDAVDEANNYLPGWLNDPLLIDSELGEVSLMEQRFWKDLIKQYLKPLELSREQKQAMADGLKELRNMIAFAFVMVNAIFVLIVFLLQLKKEFLHLEWPIDPTDFVSFDRDNLMVGIYRQYKELDPIGLCFVIFFGLILIIQFLAMFFHRFATLCQLLATTQVDWFRTGGQFTDEDAATELKGSAVSIARKLQRPKRLFDDDEDGDPHYDEVLMDSLEGEHRESMVRRQTIFRLHETRDKQHSDYSNLVFNFERRFFGDDELNLKNLALNRKSVKLLQERRSAAKVNAAATPEGTPTPKAGKRPPVVPMAKKVSFTASNRNNMALYDNGGYEHTEF

Target ORF information:

RefSeq Version	NM_001144525.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitin synthase 2, transcript variant C (Chs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144525.2

ORF Insert Sequence:

ATGAGCGGAG GAGACTACGA TAGCGGTGAC TACTTCATGC GCAGTCGGAA GCAGCGGGAC 
AAGCCGTCAT TATGGGACTC CTTCCAGGAC CCGCCGTCTA AGAAGACGAG CGGATCCGAT 
GCCGACTGGA AGAAGCTCCA GTTCTGCCTT AAGCTCTTCA AGATCTGCAC CTACCTCCTT 
GTCTTCGCGG TCGTTCTTGC GACGAGCGTG TTCGCCAACT TGATTTATCT CTACATGGCC 
GGAAACACAG GTGGCACCGG GCCACGCGTG CAAGTGTGCA GCAAGTATCT ACTCCGGCAC 
GGCCAAGTGG AGGCGGTGGC CAGCGAGCTG GACCAAATCG CCTGGGTCTG GGCCCTGATC 
TTCGCCTTTG CCGCCCCCGA GCTGCTCACC TTCCTGCGCG CCCTGCGCAT CTGTATGTTC 
AAGCGCGAGC AGCGACCCAG CGGACTAGAA GTGGGACTGG TCACGCTCTT TGAACTCCTG 
CACGTCGTTG GTCTGGCCCT GCTCACCTTC ACCGTGCTGC CGGCCCTGGA TGTGACGCGT 
GGAGCGATTC TGGGCTGCTG CGTCTGCTTC GTGCCTGCCC TGCTTAACCT GCTGACGGGA 
ACGCGGGTGC GCTGGAGGTC GGCGGACAGC CTGGTCCTCG GGCTCTCCAT CCTGGCCCTT 
GTGGCGCAGG GCAGCGTCTT TCTGGTCTGG CCCTCGCTGA GCGACAGTAT GGAGGTCCAG 
CTGCTGGCCA TTCCGCTGCT CCTTATTTCG TTTCGCTGGT GGGAGAACTT CGCCAACACT 
CACGAGGCCA TCGCCTATAT CATTCGCACC ATCCGCTGTA CCCGATCCCG ATACCACACC 
TACCTGTACC TGGCGCCTCT GAAGGTGTTG GTTTTCGCGG GGATGGGCTT CATCCTGCAT 
GGCCACGAAT TCGTTGAGTA CTTTGGTAGG TTCCGGGATG CCTGGCGGCC GCATCTCATC 
ACGCTGGTGC AATCTAATGG CGACCCCGAT GCGCTGCTTT TAGCACAGGC CAACGTATCA 
ATCACAGCCC CTCCACGCAG GACGCTGTTC ACCATGCAGT CTAGTCCGAA TTCGGTTTTC 
TACGCACTGG CGGCCCAGGT GGGTGCTGCG TACCTGTGCC ACATTTTCGG TAAGTTCGCC 
TGCAAAATCA AGATCCAGAA GTTCAGCTAC GCGCTGCCCC TCAGCCTGGC AGGACCTGCC 
ACCGTCTGCC TGGTCACCTT TCTGGCCCAG CTGCGGGCCT CGGACCCGTG CTCCCTGCAC 
GGTTTCATGC CGGACTACCT CTCGCTCCTG GCGCTGGGCG GGAGTGTGGA GGAGCTGGGC 
CAGAGATGCC TCGACTACGC CCTCTGGCTG TGGCCCCTGT GGTGGCTGGC CCAGGTGTGG 
ACCTGCCTGC ACATTTGGCA GCCCCACAAT GACAGGAACG CCCCAACCGA GAAGCTCTTC 
GTGTGTCCAT GGTACTGCGG CCTGCTGGTG GACCAGTGCT CGATGATGAA CAGGCGGATC 
GTGGACTGGA GCGAGGAGTA TTTGGCCATC AAGAGCGACC TAACCGCGCC GAGGGATCCT 
GTGGTGGCTC TGGCAGCCGA CATCAATGCC AGCCGGGACA TCCAGGAGGA GGATAAGGTG 
CCGCAGCTGA TTGTGTGCGC CACCATGTGG CACGAGACCG AGGACGAGAT GATGGAGTTC 
CTCAAGTCAA TTGTCCGCTT GGATGAGGAT CAATGCGCCC GCCGCATGGC CAGGACGCAC 
CTCAACGGCG GGAAGGCGGA CGACGAGTAC TATGAATTGG AAACTAACAT CTTCTTCGAC 
GATGCATTCG TTTCGGATCC GCGTCAGTGC CAGAACAAGC GTAACCCACC CATCAACGAG 
TATGTGAAGA CCCTAACCCG AACCATCGAC AAGGCAGCCT TTGAGGTCTA CGGAGTGAAC 
ATCCGCATAA AGCCCCCTCT TAAGATCGAG ACTCCCTACG GAGGCCGCTT GGTGTGGACC 
CTGCCAGGAC GCACTAAAAT GGTGGCCCAT CTGAAGAACA AGGACAAGAT CCGGCACAAG 
AAGCGCTGGT CGCAGGTCAT GTACATGTAC TATCTTCTGG GATATCGGAT CATGGAGACG 
AAGGAGCTGT CACCCCGTCG AAAGGCGGTT ATTGCAGAGA ACACCTTTAT CTTGGCCCTG 
GACGGAGACA TAGACTTCCA GCCGCGAGCT GTGAGGCTGC TGATCGATCG AATGAAGGCC 
GTGGACGAGC TGGGAGCTGC CTGCGGTCGA ATTCACCCGG TGGGCAGGGG TCCGATGGTG 
TGGTACCAGA GATTTGAGTA CGCCATTGGA CACTGGCTGC AAAAGGCGAC GGAGCATGTC 
ATTGGTTGCG TCCTCTGCAG TCCGGGATGC TTCAGTCTGT TCCGGGCCAG CGCCCTGATG 
GAGAACAGCG TGATGAAGCG GTACACGATG ATCAGTTCGG AGGCGATGAA GATGGTGCAG 
TACGACCAGG GCGAGGACCG CTGGCTGTGT ACGCTGCTCT TGAAGGCCGG CTCCCGGGTG 
GAGTACTGCG CCGCCTCGGA TGCGTATACC CACGCGCCAG AGAGCTTTAA CGAGTTCTAC 
AACCAACGAC GTCGTTGGGT GCCCTCCACT ATAGCCAACA TCTTCGATAT CCTAGCGGAT 
GCGGACACCG TGGTGAAGAA GAACAACTCC ATATCAACGA TGTACGTGTG GTACCAGGGA 
ATGCTGATGA TCGGAACCGT GTTGGGACCG GGCACCATAT TCCTCATGAT GGTGGGCGCC 
TTGGTGGCCG TGTTTTCAAT TGACATCTGG ACCGCTTTCC TATGGAATTT TTTTCCCATC 
CTCTTATTCA TTCTGGCCTG TGTCTACCTT AAACAGAAGT TTCAACTGCT GATAGCATTC 
GTCATTAGCT CCGTTTACTG CCTAGTCATG ATGGCAGTCC TCATCGGCAT CATTGTCCAG 
ATGCTGGAGG ACGGACCCCT GGCTCCTGCA TCGCTGTTCT TCCTGCTCGT AGCTGTGCAA 
ATCACAATTG CAGGCTTTAT GCATCCACAG GAGTTCTGGG CATTGCTGTG CGGAGTCATC 
TACTACATAA CCATCCCTTC GATGTACATG CTTCTCATGA TCTTTTCTGT GTTCAACATG 
AACGATGTTT CGTGGGGCAC TCGAGAGGCT CCAGTGCTCA AGGACGACGG CAAGGACGCA 
GTCGATGAAG CGAACAACTA CCTGCCAGGC TGGCTAAATG ATCCGCTATT GATCGACTCG 
GAGCTGGGCG AAGTGTCGTT GATGGAGCAG AGATTCTGGA AGGATCTGAT AAAGCAGTAT 
CTGAAGCCAT TGGAACTGAG TAGGGAGCAG AAGCAGGCGA TGGCCGACGG ACTTAAGGAG 
CTGAGAAACA TGATCGCCTT CGCCTTTGTG ATGGTCAATG CGATCTTTGT GCTCATTGTG 
TTCCTGCTGC AGCTGAAGAA GGAGTTTCTG CACCTCGAGT GGCCTATTGA TCCTACAGAC 
TTTGTATCCT TCGACAGGGA CAACCTAATG GTTGGCATCT ACAGGCAGTA CAAAGAACTG 
GATCCCATTG GCCTGTGCTT TGTGATTTTC TTTGGACTGA TCCTGATCAT CCAGTTCCTG 
GCGATGTTCT TCCACCGCTT CGCTACCCTG TGCCAGCTGC TGGCCACTAC TCAGGTGGAC 
TGGTTTCGAA CAGGAGGTCA GTTTACTGAC GAGGATGCCG CCACCGAGCT GAAGGGCAGT 
GCTGTCAGTA TAGCCCGGAA GTTGCAGCGA CCCAAGAGGC TCTTCGACGA CGACGAGGAC 
GGGGACCCCC ACTACGACGA GGTGTTGATG GATTCTTTAG AAGGGGAGCA CAGGGAGAGC 
ATGGTGCGAC GGCAGACGAT CTTTCGGCTT CACGAGACGC GGGACAAGCA GCACAGCGAC 
TACAGCAATC TGGTGTTTAA CTTTGAACGC CGTTTCTTTG GAGACGACGA GCTGAACCTT 
AAGAATCTGG CACTCAACCG AAAGTCAGTG AAGCTTCTGC AGGAGCGTCG CTCTGCGGCC 
AAGGTCAATG CAGCAGCCAC GCCCGAGGGC ACGCCAACTC CGAAGGCTGG AAAGCGACCG 
CCTGTGGTGC CGATGGCCAA GAAGGTGAGC TTTACGGCCT CAAACCGGAA CAATATGGCT 
TTGTACGACA ACGGCGGGTA CGAGCACACC GAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chs2 ( NM_079485.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524209.3 Drosophila melanogaster chitin synthase 2, transcript variant D (Chs2), mRNA.

Price & Availability↑

CloneID	OFa21236
Clone ID Related Accession (Same CDS sequence)	NM_079485.4
Accession Version	NM_079485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitin synthase 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079485.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGAG GAGACTACGA TAGCGGTGAC TACTTCATGC GCAGTCGGAA GCAGCGGGAC 
AAGCCGTCAT TATGGGACTC CTTCCAGGAC CCGCCGTCTA AGAAGACGAG CGGATCCGAT 
GCCGACTGGA AGAAGCTCCA GTTCTGCCTT AAGCTCTTCA AGATCTGCAC CTACCTCCTT 
GTCTTCGCGG TCGTTCTTGC GACGAGCGTG TTCGCCAACT TGATTTATCT CTACATGGCC 
GGAAACACAG GTGGCACCGG GCCACGCGTG CAAGTGTGCA GCAAGTATCC AGTACTCCGG 
CACGGCCAAG TGGAGGCGGT GGCCAGCGAG CTGGACCAAA TCGCCTGGGT CTGGGCCCTG 
ATCTTCGCCT TTGCCGCCCC CGAGCTGCTC ACCTTCCTGC GCGCCCTGCG CATCTGTATG 
TTCAAGCGCG AGCAGCGACC CAGCGGACTA GAAGTGGGAC TGGTCACGCT CTTTGAACTC 
CTGCACGTCG TTGGTCTGGC CCTGCTCACC TTCACCGTGC TGCCGGCCCT GGATGTGACG 
CGTGGAGCGA TTCTGGGCTG CTGCGTCTGC TTCGTGCCTG CCCTGCTTAA CCTGCTGACG 
GGAACGCGGG TGCGCTGGAG GTCGGCGGAC AGCCTGGTCC TCGGGCTCTC CATCCTGGCC 
CTTGTGGCGC AGGGCAGCGT CTTTCTGGTC TGGCCCTCGC TGAGCGACAG TATGGAGGTC 
CAGCTGCTGG CCATTCCGCT GCTCCTTATT TCGTTTCGCT GGTGGGAGAA CTTCGCCAAC 
ACTCACGAGG CCATCGCCTA TATCATTCGC ACCATCCGCT GTACCCGATC CCGATACCAC 
ACCTACCTGT ACCTGGCGCC TCTGAAGGTG TTGGTTTTCG CGGGGATGGG CTTCATCCTG 
CATGGCCACG AATTCGTTGA GTACTTTGGT AGGTTCCGGG ATGCCTGGCG GCCGCATCTC 
ATCACGCTGG TGCAATCTAA TGGCGACCCC GATGCGCTGC TTTTAGCACA GGCCAACGTA 
TCAATCACAG CCCCTCCACG CAGGACGCTG TTCACCATGC AGTCTAGTCC GAATTCGGTT 
TTCTACGCAC TGGCGGCCCA GGTGGGTGCT GCGTACCTGT GCCACATTTT CGGTAAGTTC 
GCCTGCAAAA TCAAGATCCA GAAGTTCAGC TACGCGCTGC CCCTCAGCCT GGCAGGACCT 
GCCACCGTCT GCCTGGTCAC CTTTCTGGCC CAGCTGCGGG CCTCGGACCC GTGCTCCCTG 
CACGGTTTCA TGCCGGACTA CCTCTCGCTC CTGGCGCTGG GCGGGAGTGT GGAGGAGCTG 
GGCCAGAGAT GCCTCGACTA CGCCCTCTGG CTGTGGCCCC TGTGGTGGCT GGCCCAGGTG 
TGGACCTGCC TGCACATTTG GCAGCCCCAC AATGACAGGA ACGCCCCAAC CGAGAAGCTC 
TTCGTGTGTC CATGGTACTG CGGCCTGCTG GTGGACCAGT GCTCGATGAT GAACAGGCGG 
ATCGTGGACT GGAGCGAGGA GTATTTGGCC ATCAAGAGCG ACCTAACCGC GCCGAGGGAT 
CCTGTGGTGG CTCTGGCAGC CGACATCAAT GCCAGCCGGG ACATCCAGGA GGAGGATAAG 
GTGCCGCAGC TGATTGTGTG CGCCACCATG TGGCACGAGA CCGAGGACGA GATGATGGAG 
TTCCTCAAGT CAATTGTCCG CTTGGATGAG GATCAATGCG CCCGCCGCAT GGCCAGGACG 
CACCTCAACG GCGGGAAGGC GGACGACGAG TACTATGAAT TGGAAACTAA CATCTTCTTC 
GACGATGCAT TCGTTTCGGA TCCGCGTCAG TGCCAGAACA AGCGTAACCC ACCCATCAAC 
GAGTATGTGA AGACCCTAAC CCGAACCATC GACAAGGCAG CCTTTGAGGT CTACGGAGTG 
AACATCCGCA TAAAGCCCCC TCTTAAGATC GAGACTCCCT ACGGAGGCCG CTTGGTGTGG 
ACCCTGCCAG GACGCACTAA AATGGTGGCC CATCTGAAGA ACAAGGACAA GATCCGGCAC 
AAGAAGCGCT GGTCGCAGGT CATGTACATG TACTATCTTC TGGGATATCG GATCATGGAG 
ACGAAGGAGC TGTCACCCCG TCGAAAGGCG GTTATTGCAG AGAACACCTT TATCTTGGCC 
CTGGACGGAG ACATAGACTT CCAGCCGCGA GCTGTGAGGC TGCTGATCGA TCGAATGAAG 
GCCGTGGACG AGCTGGGAGC TGCCTGCGGT CGAATTCACC CGGTGGGCAG GGGTCCGATG 
GTGTGGTACC AGAGATTTGA GTACGCCATT GGACACTGGC TGCAAAAGGC GACGGAGCAT 
GTCATTGGTT GCGTCCTCTG CAGTCCGGGA TGCTTCAGTC TGTTCCGGGC CAGCGCCCTG 
ATGGAGAACA GCGTGATGAA GCGGTACACG ATGATCAGTT CGGAGGCGAT GAAGATGGTG 
CAGTACGACC AGGGCGAGGA CCGCTGGCTG TGTACGCTGC TCTTGAAGGC CGGCTCCCGG 
GTGGAGTACT GCGCCGCCTC GGATGCGTAT ACCCACGCGC CAGAGAGCTT TAACGAGTTC 
TACAACCAAC GACGTCGTTG GGTGCCCTCC ACTATAGCCA ACATCTTCGA TATCCTAGCG 
GATGCGGACA CCGTGGTGAA GAAGAACAAC TCCATATCAA CGATGTACGT GTGGTACCAG 
GGAATGCTGA TGATCGGAAC CGTGTTGGGA CCGGGCACCA TATTCCTCAT GATGGTGGGC 
GCCTTGGTGG CCGTGTTTTC AATTGACATC TGGACCGCTT TCCTATGGAA TTTTTTTCCC 
ATCCTCTTAT TCATTCTGGC CTGTGTCTAC CTTAAACAGA AGTTTCAACT GCTGATAGCA 
TTCGTCATTA GCTCCGTTTA CTGCCTAGTC ATGATGGCAG TCCTCATCGG CATCATTGTC 
CAGATGCTGG AGGACGGACC CCTGGCTCCT GCATCGCTGT TCTTCCTGCT CGTAGCTGTG 
CAAATCACAA TTGCAGGCTT TATGCATCCA CAGGAGTTCT GGGCATTGCT GTGCGGAGTC 
ATCTACTACA TAACCATCCC TTCGATGTAC ATGCTTCTCA TGATCTTTTC TGTGTTCAAC 
ATGAACGATG TTTCGTGGGG CACTCGAGAG GCTCCAGTGC TCAAGGACGA CGGCAAGGAC 
GCAGTCGATG AAGCGAACAA CTACCTGCCA GGCTGGCTAA ATGATCCGCT ATTGATCGAC 
TCGGAGCTGG GCGAAGTGTC GTTGATGGAG CAGAGATTCT GGAAGGATCT GATAAAGCAG 
TATCTGAAGC CATTGGAACT GAGTAGGGAG CAGAAGCAGG CGATGGCCGA CGGACTTAAG 
GAGCTGAGAA ACATGATCGC CTTCGCCTTT GTGATGGTCA ATGCGATCTT TGTGCTCATT 
GTGTTCCTGC TGCAGCTGAA GAAGGAGTTT CTGCACCTCG AGTGGCCTAT TGATCCTACA 
GACTTTGTAT CCTTCGACAG GGACAACCTA ATGGTTGGCA TCTACAGGCA GTACAAAGAA 
CTGGATCCCA TTGGCCTGTG CTTTGTGATT TTCTTTGGAC TGATCCTGAT CATCCAGTTC 
CTGGCGATGT TCTTCCACCG CTTCGCTACC CTGTGCCAGC TGCTGGCCAC TACTCAGGTG 
GACTGGTTTC GAACAGGAGG TCAGTTTACT GACGAGGATG CCGCCACCGA GCTGAAGGGC 
AGTGCTGTCA GTATAGCCCG GAAGTTGCAG CGACCCAAGA GGCTCTTCGA CGACGACGAG 
GACGGGGACC CCCACTACGA CGAGGTGTTG ATGGATTCTT TAGAAGGGGA GCACAGGGAG 
AGCATGGTGC GACGGCAGAC GATCTTTCGG CTTCACGAGA CGCGGGACAA GCAGCACAGC 
GACTACAGCA ATCTGGTGTT TAACTTTGAA CGCCGTTTCT TTGGAGACGA CGAGCTGAAC 
CTTAAGAATC TGGCACTCAA CCGAAAGTCA GTGAAGCTTC TGCAGGAGCG TCGCTCTGCG 
GCCAAGGTCA ATGCAGCAGC CACGCCCGAG GGCACGCCAA CTCCGAAGGC TGGAAAGCGA 
CCGCCTGTGG TGCCGATGGC CAAGAAGGTG AGCTTTACGG CCTCAAACCG GAACAATATG 
GCTTTGTACG ACAACGGCGG GTACGAGCAC ACCGAGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524209.3
CDS	31..4212
Translation	MSGGDYDSGDYFMRSRKQRDKPSLWDSFQDPPSKKTSGSDADWKKLQFCLKLFKICTYLLVFAVVLATSVFANLIYLYMAGNTGGTGPRVQVCSKYPVLRHGQVEAVASELDQIAWVWALIFAFAAPELLTFLRALRICMFKREQRPSGLEVGLVTLFELLHVVGLALLTFTVLPALDVTRGAILGCCVCFVPALLNLLTGTRVRWRSADSLVLGLSILALVAQGSVFLVWPSLSDSMEVQLLAIPLLLISFRWWENFANTHEAIAYIIRTIRCTRSRYHTYLYLAPLKVLVFAGMGFILHGHEFVEYFGRFRDAWRPHLITLVQSNGDPDALLLAQANVSITAPPRRTLFTMQSSPNSVFYALAAQVGAAYLCHIFGKFACKIKIQKFSYALPLSLAGPATVCLVTFLAQLRASDPCSLHGFMPDYLSLLALGGSVEELGQRCLDYALWLWPLWWLAQVWTCLHIWQPHNDRNAPTEKLFVCPWYCGLLVDQCSMMNRRIVDWSEEYLAIKSDLTAPRDPVVALAADINASRDIQEEDKVPQLIVCATMWHETEDEMMEFLKSIVRLDEDQCARRMARTHLNGGKADDEYYELETNIFFDDAFVSDPRQCQNKRNPPINEYVKTLTRTIDKAAFEVYGVNIRIKPPLKIETPYGGRLVWTLPGRTKMVAHLKNKDKIRHKKRWSQVMYMYYLLGYRIMETKELSPRRKAVIAENTFILALDGDIDFQPRAVRLLIDRMKAVDELGAACGRIHPVGRGPMVWYQRFEYAIGHWLQKATEHVIGCVLCSPGCFSLFRASALMENSVMKRYTMISSEAMKMVQYDQGEDRWLCTLLLKAGSRVEYCAASDAYTHAPESFNEFYNQRRRWVPSTIANIFDILADADTVVKKNNSISTMYVWYQGMLMIGTVLGPGTIFLMMVGALVAVFSIDIWTAFLWNFFPILLFILACVYLKQKFQLLIAFVISSVYCLVMMAVLIGIIVQMLEDGPLAPASLFFLLVAVQITIAGFMHPQEFWALLCGVIYYITIPSMYMLLMIFSVFNMNDVSWGTREAPVLKDDGKDAVDEANNYLPGWLNDPLLIDSELGEVSLMEQRFWKDLIKQYLKPLELSREQKQAMADGLKELRNMIAFAFVMVNAIFVLIVFLLQLKKEFLHLEWPIDPTDFVSFDRDNLMVGIYRQYKELDPIGLCFVIFFGLILIIQFLAMFFHRFATLCQLLATTQVDWFRTGGQFTDEDAATELKGSAVSIARKLQRPKRLFDDDEDGDPHYDEVLMDSLEGEHRESMVRRQTIFRLHETRDKQHSDYSNLVFNFERRFFGDDELNLKNLALNRKSVKLLQERRSAAKVNAAATPEGTPTPKAGKRPPVVPMAKKVSFTASNRNNMALYDNGGYEHTEF

Target ORF information:

RefSeq Version	NM_079485.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitin synthase 2, transcript variant D (Chs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079485.4

ORF Insert Sequence:

ATGAGCGGAG GAGACTACGA TAGCGGTGAC TACTTCATGC GCAGTCGGAA GCAGCGGGAC 
AAGCCGTCAT TATGGGACTC CTTCCAGGAC CCGCCGTCTA AGAAGACGAG CGGATCCGAT 
GCCGACTGGA AGAAGCTCCA GTTCTGCCTT AAGCTCTTCA AGATCTGCAC CTACCTCCTT 
GTCTTCGCGG TCGTTCTTGC GACGAGCGTG TTCGCCAACT TGATTTATCT CTACATGGCC 
GGAAACACAG GTGGCACCGG GCCACGCGTG CAAGTGTGCA GCAAGTATCC AGTACTCCGG 
CACGGCCAAG TGGAGGCGGT GGCCAGCGAG CTGGACCAAA TCGCCTGGGT CTGGGCCCTG 
ATCTTCGCCT TTGCCGCCCC CGAGCTGCTC ACCTTCCTGC GCGCCCTGCG CATCTGTATG 
TTCAAGCGCG AGCAGCGACC CAGCGGACTA GAAGTGGGAC TGGTCACGCT CTTTGAACTC 
CTGCACGTCG TTGGTCTGGC CCTGCTCACC TTCACCGTGC TGCCGGCCCT GGATGTGACG 
CGTGGAGCGA TTCTGGGCTG CTGCGTCTGC TTCGTGCCTG CCCTGCTTAA CCTGCTGACG 
GGAACGCGGG TGCGCTGGAG GTCGGCGGAC AGCCTGGTCC TCGGGCTCTC CATCCTGGCC 
CTTGTGGCGC AGGGCAGCGT CTTTCTGGTC TGGCCCTCGC TGAGCGACAG TATGGAGGTC 
CAGCTGCTGG CCATTCCGCT GCTCCTTATT TCGTTTCGCT GGTGGGAGAA CTTCGCCAAC 
ACTCACGAGG CCATCGCCTA TATCATTCGC ACCATCCGCT GTACCCGATC CCGATACCAC 
ACCTACCTGT ACCTGGCGCC TCTGAAGGTG TTGGTTTTCG CGGGGATGGG CTTCATCCTG 
CATGGCCACG AATTCGTTGA GTACTTTGGT AGGTTCCGGG ATGCCTGGCG GCCGCATCTC 
ATCACGCTGG TGCAATCTAA TGGCGACCCC GATGCGCTGC TTTTAGCACA GGCCAACGTA 
TCAATCACAG CCCCTCCACG CAGGACGCTG TTCACCATGC AGTCTAGTCC GAATTCGGTT 
TTCTACGCAC TGGCGGCCCA GGTGGGTGCT GCGTACCTGT GCCACATTTT CGGTAAGTTC 
GCCTGCAAAA TCAAGATCCA GAAGTTCAGC TACGCGCTGC CCCTCAGCCT GGCAGGACCT 
GCCACCGTCT GCCTGGTCAC CTTTCTGGCC CAGCTGCGGG CCTCGGACCC GTGCTCCCTG 
CACGGTTTCA TGCCGGACTA CCTCTCGCTC CTGGCGCTGG GCGGGAGTGT GGAGGAGCTG 
GGCCAGAGAT GCCTCGACTA CGCCCTCTGG CTGTGGCCCC TGTGGTGGCT GGCCCAGGTG 
TGGACCTGCC TGCACATTTG GCAGCCCCAC AATGACAGGA ACGCCCCAAC CGAGAAGCTC 
TTCGTGTGTC CATGGTACTG CGGCCTGCTG GTGGACCAGT GCTCGATGAT GAACAGGCGG 
ATCGTGGACT GGAGCGAGGA GTATTTGGCC ATCAAGAGCG ACCTAACCGC GCCGAGGGAT 
CCTGTGGTGG CTCTGGCAGC CGACATCAAT GCCAGCCGGG ACATCCAGGA GGAGGATAAG 
GTGCCGCAGC TGATTGTGTG CGCCACCATG TGGCACGAGA CCGAGGACGA GATGATGGAG 
TTCCTCAAGT CAATTGTCCG CTTGGATGAG GATCAATGCG CCCGCCGCAT GGCCAGGACG 
CACCTCAACG GCGGGAAGGC GGACGACGAG TACTATGAAT TGGAAACTAA CATCTTCTTC 
GACGATGCAT TCGTTTCGGA TCCGCGTCAG TGCCAGAACA AGCGTAACCC ACCCATCAAC 
GAGTATGTGA AGACCCTAAC CCGAACCATC GACAAGGCAG CCTTTGAGGT CTACGGAGTG 
AACATCCGCA TAAAGCCCCC TCTTAAGATC GAGACTCCCT ACGGAGGCCG CTTGGTGTGG 
ACCCTGCCAG GACGCACTAA AATGGTGGCC CATCTGAAGA ACAAGGACAA GATCCGGCAC 
AAGAAGCGCT GGTCGCAGGT CATGTACATG TACTATCTTC TGGGATATCG GATCATGGAG 
ACGAAGGAGC TGTCACCCCG TCGAAAGGCG GTTATTGCAG AGAACACCTT TATCTTGGCC 
CTGGACGGAG ACATAGACTT CCAGCCGCGA GCTGTGAGGC TGCTGATCGA TCGAATGAAG 
GCCGTGGACG AGCTGGGAGC TGCCTGCGGT CGAATTCACC CGGTGGGCAG GGGTCCGATG 
GTGTGGTACC AGAGATTTGA GTACGCCATT GGACACTGGC TGCAAAAGGC GACGGAGCAT 
GTCATTGGTT GCGTCCTCTG CAGTCCGGGA TGCTTCAGTC TGTTCCGGGC CAGCGCCCTG 
ATGGAGAACA GCGTGATGAA GCGGTACACG ATGATCAGTT CGGAGGCGAT GAAGATGGTG 
CAGTACGACC AGGGCGAGGA CCGCTGGCTG TGTACGCTGC TCTTGAAGGC CGGCTCCCGG 
GTGGAGTACT GCGCCGCCTC GGATGCGTAT ACCCACGCGC CAGAGAGCTT TAACGAGTTC 
TACAACCAAC GACGTCGTTG GGTGCCCTCC ACTATAGCCA ACATCTTCGA TATCCTAGCG 
GATGCGGACA CCGTGGTGAA GAAGAACAAC TCCATATCAA CGATGTACGT GTGGTACCAG 
GGAATGCTGA TGATCGGAAC CGTGTTGGGA CCGGGCACCA TATTCCTCAT GATGGTGGGC 
GCCTTGGTGG CCGTGTTTTC AATTGACATC TGGACCGCTT TCCTATGGAA TTTTTTTCCC 
ATCCTCTTAT TCATTCTGGC CTGTGTCTAC CTTAAACAGA AGTTTCAACT GCTGATAGCA 
TTCGTCATTA GCTCCGTTTA CTGCCTAGTC ATGATGGCAG TCCTCATCGG CATCATTGTC 
CAGATGCTGG AGGACGGACC CCTGGCTCCT GCATCGCTGT TCTTCCTGCT CGTAGCTGTG 
CAAATCACAA TTGCAGGCTT TATGCATCCA CAGGAGTTCT GGGCATTGCT GTGCGGAGTC 
ATCTACTACA TAACCATCCC TTCGATGTAC ATGCTTCTCA TGATCTTTTC TGTGTTCAAC 
ATGAACGATG TTTCGTGGGG CACTCGAGAG GCTCCAGTGC TCAAGGACGA CGGCAAGGAC 
GCAGTCGATG AAGCGAACAA CTACCTGCCA GGCTGGCTAA ATGATCCGCT ATTGATCGAC 
TCGGAGCTGG GCGAAGTGTC GTTGATGGAG CAGAGATTCT GGAAGGATCT GATAAAGCAG 
TATCTGAAGC CATTGGAACT GAGTAGGGAG CAGAAGCAGG CGATGGCCGA CGGACTTAAG 
GAGCTGAGAA ACATGATCGC CTTCGCCTTT GTGATGGTCA ATGCGATCTT TGTGCTCATT 
GTGTTCCTGC TGCAGCTGAA GAAGGAGTTT CTGCACCTCG AGTGGCCTAT TGATCCTACA 
GACTTTGTAT CCTTCGACAG GGACAACCTA ATGGTTGGCA TCTACAGGCA GTACAAAGAA 
CTGGATCCCA TTGGCCTGTG CTTTGTGATT TTCTTTGGAC TGATCCTGAT CATCCAGTTC 
CTGGCGATGT TCTTCCACCG CTTCGCTACC CTGTGCCAGC TGCTGGCCAC TACTCAGGTG 
GACTGGTTTC GAACAGGAGG TCAGTTTACT GACGAGGATG CCGCCACCGA GCTGAAGGGC 
AGTGCTGTCA GTATAGCCCG GAAGTTGCAG CGACCCAAGA GGCTCTTCGA CGACGACGAG 
GACGGGGACC CCCACTACGA CGAGGTGTTG ATGGATTCTT TAGAAGGGGA GCACAGGGAG 
AGCATGGTGC GACGGCAGAC GATCTTTCGG CTTCACGAGA CGCGGGACAA GCAGCACAGC 
GACTACAGCA ATCTGGTGTT TAACTTTGAA CGCCGTTTCT TTGGAGACGA CGAGCTGAAC 
CTTAAGAATC TGGCACTCAA CCGAAAGTCA GTGAAGCTTC TGCAGGAGCG TCGCTCTGCG 
GCCAAGGTCA ATGCAGCAGC CACGCCCGAG GGCACGCCAA CTCCGAAGGC TGGAAAGCGA 
CCGCCTGTGG TGCCGATGGC CAAGAAGGTG AGCTTTACGG CCTCAAACCG GAACAATATG 
GCTTTGTACG ACAACGGCGG GTACGAGCAC ACCGAGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Chs2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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