ints7 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ints7
Entrez Gene ID	286776
Full Name	integrator complex subunit 7
Synonyms	chunp6916
General protein information	Preferred Names integrator complex subunit 7 Names integrator complex subunit 7 int7 un-named hi1548 unm hi1548
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468353.1	XP_021324028.1	integrator complex subunit 7 isoform X1
NM_173267.1	NP_775374.1	integrator complex subunit 7

Pathways from BioSystems

Homologies

	INTS7	XP_001108251.1
Rattus norvegicus (Norway rat)	Ints7	NP_001178604.1
Gallus gallus (chicken)	INTS7	NP_001006399.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011393	XP_317927.4
Mus musculus (house mouse)	Ints7	NP_848747.4
Drosophila melanogaster (fruit fly)	defl	NP_648352.2
Bos taurus (cattle)	INTS7	NP_001069512.1
Homo sapiens (human)	INTS7	NP_056249.1
Pan troglodytes (chimpanzee)	INTS7	XP_514179.4
Canis lupus familiaris (dog)	INTS7	XP_857568.2
Danio rerio (zebrafish)	ints7	NP_775374.1
Xenopus tropicalis (tropical clawed frog)	ints7	XP_002934743.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Many genes in fish have species-specific asymmetric rates of molecular evolution.
Steinke D, Salzburger W, Braasch I, Meyer A
BMC genomics720(2006 Feb)

Identification of zebrafish insertional mutants with defects in visual system development and function.
Gross JM, Perkins BD, Amsterdam A, Ega?a A, Darland T, Matsui JI, Sciascia S, Hopkins N, Dowling JE
Genetics170(1)245-61(2005 May)

Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development.
Golling G, Amsterdam A, Sun Z, Antonelli M, Maldonado E, Chen W, Burgess S, Haldi M, Artzt K, Farrington S, Lin SY, Nissen RM, Hopkins N
Nature genetics31(2)135-40(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ints7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ints7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38580	NM_173267.1 Latest version!	Danio rerio integrator complex subunit 7 (ints7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa150574	XM_021468353.1 Latest version!	Danio rerio integrator complex subunit 7 (ints7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ints7 ( NM_173267.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775374.1 Danio rerio integrator complex subunit 7 (ints7), mRNA.

Price & Availability↑

CloneID	ODa38580
Clone ID Related Accession (Same CDS sequence)	NM_173267.1
Accession Version	NM_173267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	integrator complex subunit 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY099529.1. ##Evidence-Data-START## Transcript exon combination :: AY099529.1, BC163911.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCGCTTT CAGCAGCGCG TTCGTTTCTT TCGGAGGCGG CATACGGCGA GCAGGAGCTG 
GACGCCAATT CAGCGCTGAT GGAGCTCGAT AAAGGTTTAC GGTCTTGTAA ACTGGGCGAG 
CAGTGTGAAG CCGTGGTCCT TTTTCCCAAA CTCTTCCAGA AATACCCGTT CCCCATCCTC 
ATCAACTCTG CCTTCCTGAA GCTGGCAGAT ATCTTCAGAC TTGGCAACAA TTTCCTGCGG 
CTGTGTGTGC TGAAGGTCAC GCAGCTGAGT GAAAAACACT TGGAGAAGAT CCTGAATGTG 
GATGAGTTTG TCAAAAGAGT GTTTTCTGTC ATCCACAGCA ATGACCCTGT GGCCCGAGCA 
ATCACCCTCA GAATGCTGGG AAGTTTAGCT TCCATCATTC CAGAGAGAAA GAACGCTCAC 
CACAGCATCC GCCAAAGCCT GGACTCTCAT GATAATGTTG AAGTAGAAGC TGCTATTTTT 
GCAGCCGCTA GCTTCTCATC ACACTCCAAA GATTTTGCAG CAGGAATTTG CAACAAGATT 
AGTGAGATGA TTCAAGGACT GGACACTCCA GTAGAGCTAA AGCTGAAGTT GATCCCGATG 
CTGCAGCACA TGCACCATGA TGCCAGCCTG GCGTCGTGCA GCAGAGAGTT ACTGCAGGAG 
CTGGTGTCCT CGTACCCATC CACATCCATG CTGATCGTCA CACTGCACAC CTTTACCCAG 
CTGGCCACAT CTTCACTCGT TGACATACCG GAGCAGATTT GTCTTCTTCT GCAGTACTTG 
AAGGAAGACC CCAGAAAAGC TGTAAAAAGA CTCTCCATTC AGGATCTCAA GCTCCTGGCC 
AAAAAGGCTC CTCATTTGTG GACCAGGAAA AACATACAGG TGCTGTGCGA GTGTGCACTC 
CACACCCCAT ATAACAGCCT GAAGCTGGGA ATGCTGTCTG TCCTCTCCAC ATTGTCTGGC 
ACCATCGCTA TTAAACAGTA CTTCAGCCCT AATGCAGGTG ATTCATCTCC TGCACCTCAC 
CACACTGACC TGGTAAAGCT GGCACAGGAG TGCTGTTATC ACAGTGACCT GGCAGTTGCA 
GCACATGGAA TCACAGTGTT GACTAGCATT GCTGCTTTCT GCCCTGAGAA AGAGGTCATT 
CAGCTGGAAC AGGAAACAGT GATGGGGATG GAGTCTCTCA TTCTGCTCTG CAGTCAAGAT 
GACAGTAAGA CTGCCCAGGC CACACTAAAA ACGGCGCTCA CGTCTCTGGT GCAGATGTTG 
AAGACTTGTC CTCATCTCAG TCAGAGCTCA GTGGAGCTCC TGCTTAGGCA GCTGCATTGC 
GCATGTGACC CTGCTCGGGT GCTCATGTGT CAGGCTCTGG CTGCTATTGC CACACAACAG 
CCAGTGCTGG TAGAGGGAAT GCTGGGAGAC CTGCTGGAGC TCTTCAGGGT GGCAAGTCAC 
AGGACTTCAG AGAAACAGCA GGAGCTTCTG GTGTCTTTGG CAACTGTGTT ATTTGTGGCC 
AGTCAGGCGT CTCTCTCAGC TGAGGTGAAG GCTGTGATCA GACAACAGTT GGAGAATGTG 
GCCAATGGCT GGACCGTGTA CCAAATTGCC AGACAAGCGT CCCGCATGGG CTGCCATGAT 
TTTTCCAGGG AGTTGTACCA GAGTCTTCGC ACCCGTGTGG CCTCCGAACA CTTTTACTTC 
TGGCTTAACA GTCTGATGGA GTTTTCCCAA GCGGAGCAGT GCCTTAGTGG CCTGGAAGAT 
GGAGACTACA GTGCAGCCAT GAGCGCCATC TCTGAAGCAC TGAAATCCTA CCAGAAGGGA 
ATCGCATCTC TCACGGCTGC CAGTACGCCG CTCAGTCCGC TGACATTCCA ATGTGAGTTT 
GTGAAGCTGC GTATAGACAC TCTTCAGGCT CTGTCTCAGC TCATTTGCAC CTGCAACAGT 
CTGAAGACCA GTCCTCCTCC TGCCATCGCA ACCACGATCG CTCTGTCCTC GGGCAGTGAC 
TTGCAGCGCT GCGGACGAAT CTCCACACAG ATGAAGTTTT CCATGGATGA GTTTAGAAGT 
CTCGCAGCTC GCTATGCTGA CCTGTACCAG TCTTCGTTTG ATGCGGACTA TGCCACCCTT 
CGCAATGTTG AACTACAACA GCAGAGTTGC TTACTCGTAT CTTATGTTAT CGAAGCTTTA 
ATAATCGATC CACAAACAGC CAGTTTCCAG GAGTTTGGCA CTCATGGATC AATTCTGGCA 
GAGAGCGAGT ATGAGCTGAG AATGATGGCT GTGTTCAATC ATGTCCTGGA GGAAGTGGAA 
AATCTAAGCA GGAAACACCC TCCTGTTTCT TACCTGCACA CCGGCTGTCT GTGTGATACT 
GTCATAGCCA TCCTGAAAAT ACCACTGTCG TTCCAGAGAT ATTTCTTTCA GAAGCTTCAG 
TCCACCAGCA TTAAACTTGC TCTCTCTCCA TCTCCCCGCA CACCCAATGA GCCGATCCCA 
GTGCAGAACA ACCAGCAGCT GACCTTAAAA GTGGAGGGTG TTATTCAGCA CGGCTCCAGT 
CCGGGACTCT TCCGGAAGAT TCAGGCAGTC TGTCTTAAAG TCAGCTCGAC GTTACAGACA 
AAACCAGGAT CAGACTTCAA GATTCCTCTG GAATCCAAAA CCAACGAGAT TGAACAGAAA 
GTAGAGCCTC ATAACGATTA CTTCAGCACA CAGTTCCTGC TCAACTTCTC CATCCTGGGC 
ACACACCAGG TATCTGTTGA AGCCTCAGTG GTGGACACCA GTGGGATTGA ATGGAAGACC 
GGCCCTAAAA ACACTGTTTC GGTGAAGTCT CTGGAAGACC CATATTCCCA GCAGCTTCGG 
CATCAATTAC AGCAGCAGCA GCAGAACGTC CCGCAGCCTG CAGCCCAGAG GAACATCAGC 
ACACGCTTCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775374.1
CDS	99..2993
Translation	MSLSAARSFLSEAAYGEQELDANSALMELDKGLRSCKLGEQCEAVVLFPKLFQKYPFPILINSAFLKLADIFRLGNNFLRLCVLKVTQLSEKHLEKILNVDEFVKRVFSVIHSNDPVARAITLRMLGSLASIIPERKNAHHSIRQSLDSHDNVEVEAAIFAAASFSSHSKDFAAGICNKISEMIQGLDTPVELKLKLIPMLQHMHHDASLASCSRELLQELVSSYPSTSMLIVTLHTFTQLATSSLVDIPEQICLLLQYLKEDPRKAVKRLSIQDLKLLAKKAPHLWTRKNIQVLCECALHTPYNSLKLGMLSVLSTLSGTIAIKQYFSPNAGDSSPAPHHTDLVKLAQECCYHSDLAVAAHGITVLTSIAAFCPEKEVIQLEQETVMGMESLILLCSQDDSKTAQATLKTALTSLVQMLKTCPHLSQSSVELLLRQLHCACDPARVLMCQALAAIATQQPVLVEGMLGDLLELFRVASHRTSEKQQELLVSLATVLFVASQASLSAEVKAVIRQQLENVANGWTVYQIARQASRMGCHDFSRELYQSLRTRVASEHFYFWLNSLMEFSQAEQCLSGLEDGDYSAAMSAISEALKSYQKGIASLTAASTPLSPLTFQCEFVKLRIDTLQALSQLICTCNSLKTSPPPAIATTIALSSGSDLQRCGRISTQMKFSMDEFRSLAARYADLYQSSFDADYATLRNVELQQQSCLLVSYVIEALIIDPQTASFQEFGTHGSILAESEYELRMMAVFNHVLEEVENLSRKHPPVSYLHTGCLCDTVIAILKIPLSFQRYFFQKLQSTSIKLALSPSPRTPNEPIPVQNNQQLTLKVEGVIQHGSSPGLFRKIQAVCLKVSSTLQTKPGSDFKIPLESKTNEIEQKVEPHNDYFSTQFLLNFSILGTHQVSVEASVVDTSGIEWKTGPKNTVSVKSLEDPYSQQLRHQLQQQQQNVPQPAAQRNISTRFQ

Target ORF information:

RefSeq Version	NM_173267.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrator complex subunit 7 (ints7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173267.1

ORF Insert Sequence:

ATGTCGCTTT CAGCAGCGCG TTCGTTTCTT TCGGAGGCGG CATACGGCGA GCAGGAGCTG 
GACGCCAATT CAGCGCTGAT GGAGCTCGAT AAAGGTTTAC GGTCTTGTAA ACTGGGCGAG 
CAGTGTGAAG CCGTGGTCCT TTTTCCCAAA CTCTTCCAGA AATACCCGTT CCCCATCCTC 
ATCAACTCTG CCTTCCTGAA GCTGGCAGAT ATCTTCAGAC TTGGCAACAA TTTCCTGCGG 
CTGTGTGTGC TGAAGGTCAC GCAGCTGAGT GAAAAACACT TGGAGAAGAT CCTGAATGTG 
GATGAGTTTG TCAAAAGAGT GTTTTCTGTC ATCCACAGCA ATGACCCTGT GGCCCGAGCA 
ATCACCCTCA GAATGCTGGG AAGTTTAGCT TCCATCATTC CAGAGAGAAA GAACGCTCAC 
CACAGCATCC GCCAAAGCCT GGACTCTCAT GATAATGTTG AAGTAGAAGC TGCTATTTTT 
GCAGCCGCTA GCTTCTCATC ACACTCCAAA GATTTTGCAG CAGGAATTTG CAACAAGATT 
AGTGAGATGA TTCAAGGACT GGACACTCCA GTAGAGCTAA AGCTGAAGTT GATCCCGATG 
CTGCAGCACA TGCACCATGA TGCCAGCCTG GCGTCGTGCA GCAGAGAGTT ACTGCAGGAG 
CTGGTGTCCT CGTACCCATC CACATCCATG CTGATCGTCA CACTGCACAC CTTTACCCAG 
CTGGCCACAT CTTCACTCGT TGACATACCG GAGCAGATTT GTCTTCTTCT GCAGTACTTG 
AAGGAAGACC CCAGAAAAGC TGTAAAAAGA CTCTCCATTC AGGATCTCAA GCTCCTGGCC 
AAAAAGGCTC CTCATTTGTG GACCAGGAAA AACATACAGG TGCTGTGCGA GTGTGCACTC 
CACACCCCAT ATAACAGCCT GAAGCTGGGA ATGCTGTCTG TCCTCTCCAC ATTGTCTGGC 
ACCATCGCTA TTAAACAGTA CTTCAGCCCT AATGCAGGTG ATTCATCTCC TGCACCTCAC 
CACACTGACC TGGTAAAGCT GGCACAGGAG TGCTGTTATC ACAGTGACCT GGCAGTTGCA 
GCACATGGAA TCACAGTGTT GACTAGCATT GCTGCTTTCT GCCCTGAGAA AGAGGTCATT 
CAGCTGGAAC AGGAAACAGT GATGGGGATG GAGTCTCTCA TTCTGCTCTG CAGTCAAGAT 
GACAGTAAGA CTGCCCAGGC CACACTAAAA ACGGCGCTCA CGTCTCTGGT GCAGATGTTG 
AAGACTTGTC CTCATCTCAG TCAGAGCTCA GTGGAGCTCC TGCTTAGGCA GCTGCATTGC 
GCATGTGACC CTGCTCGGGT GCTCATGTGT CAGGCTCTGG CTGCTATTGC CACACAACAG 
CCAGTGCTGG TAGAGGGAAT GCTGGGAGAC CTGCTGGAGC TCTTCAGGGT GGCAAGTCAC 
AGGACTTCAG AGAAACAGCA GGAGCTTCTG GTGTCTTTGG CAACTGTGTT ATTTGTGGCC 
AGTCAGGCGT CTCTCTCAGC TGAGGTGAAG GCTGTGATCA GACAACAGTT GGAGAATGTG 
GCCAATGGCT GGACCGTGTA CCAAATTGCC AGACAAGCGT CCCGCATGGG CTGCCATGAT 
TTTTCCAGGG AGTTGTACCA GAGTCTTCGC ACCCGTGTGG CCTCCGAACA CTTTTACTTC 
TGGCTTAACA GTCTGATGGA GTTTTCCCAA GCGGAGCAGT GCCTTAGTGG CCTGGAAGAT 
GGAGACTACA GTGCAGCCAT GAGCGCCATC TCTGAAGCAC TGAAATCCTA CCAGAAGGGA 
ATCGCATCTC TCACGGCTGC CAGTACGCCG CTCAGTCCGC TGACATTCCA ATGTGAGTTT 
GTGAAGCTGC GTATAGACAC TCTTCAGGCT CTGTCTCAGC TCATTTGCAC CTGCAACAGT 
CTGAAGACCA GTCCTCCTCC TGCCATCGCA ACCACGATCG CTCTGTCCTC GGGCAGTGAC 
TTGCAGCGCT GCGGACGAAT CTCCACACAG ATGAAGTTTT CCATGGATGA GTTTAGAAGT 
CTCGCAGCTC GCTATGCTGA CCTGTACCAG TCTTCGTTTG ATGCGGACTA TGCCACCCTT 
CGCAATGTTG AACTACAACA GCAGAGTTGC TTACTCGTAT CTTATGTTAT CGAAGCTTTA 
ATAATCGATC CACAAACAGC CAGTTTCCAG GAGTTTGGCA CTCATGGATC AATTCTGGCA 
GAGAGCGAGT ATGAGCTGAG AATGATGGCT GTGTTCAATC ATGTCCTGGA GGAAGTGGAA 
AATCTAAGCA GGAAACACCC TCCTGTTTCT TACCTGCACA CCGGCTGTCT GTGTGATACT 
GTCATAGCCA TCCTGAAAAT ACCACTGTCG TTCCAGAGAT ATTTCTTTCA GAAGCTTCAG 
TCCACCAGCA TTAAACTTGC TCTCTCTCCA TCTCCCCGCA CACCCAATGA GCCGATCCCA 
GTGCAGAACA ACCAGCAGCT GACCTTAAAA GTGGAGGGTG TTATTCAGCA CGGCTCCAGT 
CCGGGACTCT TCCGGAAGAT TCAGGCAGTC TGTCTTAAAG TCAGCTCGAC GTTACAGACA 
AAACCAGGAT CAGACTTCAA GATTCCTCTG GAATCCAAAA CCAACGAGAT TGAACAGAAA 
GTAGAGCCTC ATAACGATTA CTTCAGCACA CAGTTCCTGC TCAACTTCTC CATCCTGGGC 
ACACACCAGG TATCTGTTGA AGCCTCAGTG GTGGACACCA GTGGGATTGA ATGGAAGACC 
GGCCCTAAAA ACACTGTTTC GGTGAAGTCT CTGGAAGACC CATATTCCCA GCAGCTTCGG 
CATCAATTAC AGCAGCAGCA GCAGAACGTC CCGCAGCCTG CAGCCCAGAG GAACATCAGC 
ACACGCTTCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ints7 ( XM_021468353.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324028.1 Danio rerio integrator complex subunit 7 (ints7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150574
Clone ID Related Accession (Same CDS sequence)	XM_021468353.1
Accession Version	XM_021468353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	integrator complex subunit 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTTT CAGCAGCGCG TTCGTTTCTT TCGGAGGCGG CATACGGCGA GCAGGAGCTG 
GACGCCAATT CAGCGCTGAT GGAGCTCGAT AAAGGTTTAC GGTCTTGTAA ACTGGGCGAG 
CAGTGTGAAG CCGTGGTCCT TTTTCCCAAA CTCTTCCAGA AATACCCGTT CCCCATCCTC 
ATCAACTCTG CCTTCCTGAA GCTGGCAGAT ATCTTCAGAC TTGGCAACAA TTTCCTGCGG 
CTGTGTGTGC TGAAGGTCAC GCAGCTGAGT GAAAAACACT TGGAGAAGAT CCTGAATGTG 
GATGAGTTTG TCAAAAGAGT GTTTTCTGTC ATCCACAGCA ATGACCCTGT GGCCCGAGCA 
ATCACCCTCA GAATGCTGGG AAGTTTAGCT TCCATCATTC CAGAGAGAAA GAACGCTCAC 
CACAGCATCC GCCAAAGCCT GGACTCTCAT GATAATGTTG AAGTAGAAGC TGCTATTTTT 
GCAGCCGCTA GCTTCTCATC ACACTCCAAA GATTTTGCAG CAGGAATTTG CAACAAGATT 
AGTGAGATGA TTCAAGGACT GGACACTCCA GTAGAGCTAA AGCTGAAGTT GATCCCGATG 
CTGCAGCACA TGCACCATGA TGCCAGCCTG GCGTCGTGCA GCAGAGAGTT ACTGCAGGAG 
CTGGTGTCCT CGTACCCATC CACATCCATG CTGATCGTCA CACTGCACAC CTTTACCCAG 
CTGGCCACAT CTTCACTCGT TGACATACCG GAGCAGATTT GTCTTCTTCT GCAGTACTTG 
AAGGAAGACC CCAGAAAAGC TGTAAAAAGA CTCTCCATTC AGGATCTCAA GCTCCTGGCC 
AAAAAGGCTC CTCATTTGTG GACCAGGAAA AACATACAGG TGCTGTGCGA GTGTGCACTC 
CACACCCCAT ATAACAGCCT GAAGCTGGGA ATGCTGTCTG TCCTCTCCAC ATTGTCTGGC 
ACCATCGCTA TTAAACAGTA CTTCAGCCCT AATGCAGGTG ATTCATCTCC TGCACCTCAC 
CACACTGACC TGGTAAAGCT GGCACAGGAG TGCTGTTATC ACAGTGACCT GGCAGTTGCA 
GCACATGGAA TCACAGTGTT GACTAGCATT GCTGCTTTCT GCCCTGAGAA AGAGGTCATT 
CAGCTGGAAC AGGAAACAGT GATGGGGATG GAGTCTCTCA TTCTGCTCTG CAGTCAAGAT 
GACAGTAAGA CTGCCCAGGC CACACTAAAA ACGGCGCTCA CGTCTCTGGT GCAGATGTTG 
AAGACTTGTC CTCATCTCAG TCAGAGCTCA GTGGAGCTCC TGCTTAGGCA GCTGCATTGC 
GCATGTGACC CTGCTCGGGT GCTCATGTGT CAGGCTCTGG CTGCTATTGC CACACAACAG 
CCAGTGCTGG TAGAGGGAAT GCTGGGAGAC CTGCTGGAGC TCTTCAGGGT GGCAAGTCAC 
AGGACTTCAG AGAAACAGCA GGAGCTTCTG GTGTCTTTGG CAACTGTGTT ATTTGTGGCC 
AGTCAGGCGT CTCTCTCAGC TGAGGTGAAG GCTGTGATCA GACAACAGTT GGAGAATGTG 
GCCAATGGCT GGACCGTGTA CCAAATTGCC AGACAAGCGT CCCGCATGGG CTGCCATGAT 
TTTTCCAGGG AGTTGTACCA GAGTCTTCGC ACCCGTGTGG CCTCCGAACA CTTTTACTTC 
TGGCTTAACA GTCTGATGGA GTTTTCCCAA GCGGAGCAGT GCCTTAGTGG CCTGGAAGAT 
GGAGACTACA GTGCAGCCAT GAGCGCCATC TCTGAAGCAC TGAAATCCTA CCAGAAGGGA 
ATCGCATCTC TCACGGCTGC CAGTACGCCG CTCAGTCCGC TGACATTCCA ATGTGAGTTT 
GTGAAGCTGC GTATAGACAC TCTTCAGGCT CTGTCTCAGC TCATTTGCAC CTGCAACAGT 
CTGAAGACCA GTCCTCCTCC TGCCATCGCA ACCACGATCG CTCTGTCCTC GGGCAGTGAC 
TTGCAGCGCT GCGGACGAAT CTCCACACAG ATGAAGTTTT CCATGGATGA GTTTAGAAGT 
CTCGCAGCTC GCTATGCTGA CCTGTACCAG TCTTCGTTTG ATGCGGACTA TGCCACCCTT 
CGCAATGTTG AACTACAACA GCAGAGTTGC TTACTCGTAT CTTATGTTAT CGAAGCTTTA 
ATAATCGATC CACAAACAGC CAGTTTCCAG GAGTTTGGCA CTCATGGATC AATTCTGGCA 
GAGAGCGAGT ATGAGCTGAG AATGATGGCT GTGTTCAATC ATGTCCTGGA GGAAGTGGAA 
AATCTAAGCA GGAAACACCC TCCTGTTTCT TACCTGCTTG CTCTCTCTCC ATCTCCCCGC 
ACACCCAATG AGCCGATCCC AGTGCAGAAC AACCAGCAGC TGACCTTAAA AGTGGAGGGT 
GTTATTCAGC ACGGCTCCAG TCCGGGACTC TTCCGGAAGA TTCAGGCAGT CTGTCTTAAA 
GTCAGCTCGA CGTTACAGAC AAAACCAGGA TCAGACTTCA AGATTCCTCT GGAATCCAAA 
ACCAACGAGA TTGAACAGAA AGTAGAGCCT CATAACGATT ACTTCAGCAC ACAGTTCCTG 
CTCAACTTCT CCATCCTGGG CACACACCAG GTATCTGTTG AAGCCTCAGT GGTGGACACC 
AGTGGGATTG AATGGAAGAC CGGCCCTAAA AACACTGTTT CGGTGAAGTC TCTGGAAGAC 
CCATATTCCC AGCAGCTTCG GCATCAATTA CAGCAGCAGC AGCAGAACGT CCCGCAGCCT 
GCAGCCCAGA GGAACATCAG CACACGCTTC CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324028.1
CDS	195..2990
Translation	MSLSAARSFLSEAAYGEQELDANSALMELDKGLRSCKLGEQCEAVVLFPKLFQKYPFPILINSAFLKLADIFRLGNNFLRLCVLKVTQLSEKHLEKILNVDEFVKRVFSVIHSNDPVARAITLRMLGSLASIIPERKNAHHSIRQSLDSHDNVEVEAAIFAAASFSSHSKDFAAGICNKISEMIQGLDTPVELKLKLIPMLQHMHHDASLASCSRELLQELVSSYPSTSMLIVTLHTFTQLATSSLVDIPEQICLLLQYLKEDPRKAVKRLSIQDLKLLAKKAPHLWTRKNIQVLCECALHTPYNSLKLGMLSVLSTLSGTIAIKQYFSPNAGDSSPAPHHTDLVKLAQECCYHSDLAVAAHGITVLTSIAAFCPEKEVIQLEQETVMGMESLILLCSQDDSKTAQATLKTALTSLVQMLKTCPHLSQSSVELLLRQLHCACDPARVLMCQALAAIATQQPVLVEGMLGDLLELFRVASHRTSEKQQELLVSLATVLFVASQASLSAEVKAVIRQQLENVANGWTVYQIARQASRMGCHDFSRELYQSLRTRVASEHFYFWLNSLMEFSQAEQCLSGLEDGDYSAAMSAISEALKSYQKGIASLTAASTPLSPLTFQCEFVKLRIDTLQALSQLICTCNSLKTSPPPAIATTIALSSGSDLQRCGRISTQMKFSMDEFRSLAARYADLYQSSFDADYATLRNVELQQQSCLLVSYVIEALIIDPQTASFQEFGTHGSILAESEYELRMMAVFNHVLEEVENLSRKHPPVSYLLALSPSPRTPNEPIPVQNNQQLTLKVEGVIQHGSSPGLFRKIQAVCLKVSSTLQTKPGSDFKIPLESKTNEIEQKVEPHNDYFSTQFLLNFSILGTHQVSVEASVVDTSGIEWKTGPKNTVSVKSLEDPYSQQLRHQLQQQQQNVPQPAAQRNISTRFQ

Target ORF information:

RefSeq Version	XM_021468353.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrator complex subunit 7 (ints7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468353.1

ORF Insert Sequence:

ATGTCGCTTT CAGCAGCGCG TTCGTTTCTT TCGGAGGCGG CATACGGCGA GCAGGAGCTG 
GACGCCAATT CAGCGCTGAT GGAGCTCGAT AAAGGTTTAC GGTCTTGTAA ACTGGGCGAG 
CAGTGTGAAG CCGTGGTCCT TTTTCCCAAA CTCTTCCAGA AATACCCGTT CCCCATCCTC 
ATCAACTCTG CCTTCCTGAA GCTGGCAGAT ATCTTCAGAC TTGGCAACAA TTTCCTGCGG 
CTGTGTGTGC TGAAGGTCAC GCAGCTGAGT GAAAAACACT TGGAGAAGAT CCTGAATGTG 
GATGAGTTTG TCAAAAGAGT GTTTTCTGTC ATCCACAGCA ATGACCCTGT GGCCCGAGCA 
ATCACCCTCA GAATGCTGGG AAGTTTAGCT TCCATCATTC CAGAGAGAAA GAACGCTCAC 
CACAGCATCC GCCAAAGCCT GGACTCTCAT GATAATGTTG AAGTAGAAGC TGCTATTTTT 
GCAGCCGCTA GCTTCTCATC ACACTCCAAA GATTTTGCAG CAGGAATTTG CAACAAGATT 
AGTGAGATGA TTCAAGGACT GGACACTCCA GTAGAGCTAA AGCTGAAGTT GATCCCGATG 
CTGCAGCACA TGCACCATGA TGCCAGCCTG GCGTCGTGCA GCAGAGAGTT ACTGCAGGAG 
CTGGTGTCCT CGTACCCATC CACATCCATG CTGATCGTCA CACTGCACAC CTTTACCCAG 
CTGGCCACAT CTTCACTCGT TGACATACCG GAGCAGATTT GTCTTCTTCT GCAGTACTTG 
AAGGAAGACC CCAGAAAAGC TGTAAAAAGA CTCTCCATTC AGGATCTCAA GCTCCTGGCC 
AAAAAGGCTC CTCATTTGTG GACCAGGAAA AACATACAGG TGCTGTGCGA GTGTGCACTC 
CACACCCCAT ATAACAGCCT GAAGCTGGGA ATGCTGTCTG TCCTCTCCAC ATTGTCTGGC 
ACCATCGCTA TTAAACAGTA CTTCAGCCCT AATGCAGGTG ATTCATCTCC TGCACCTCAC 
CACACTGACC TGGTAAAGCT GGCACAGGAG TGCTGTTATC ACAGTGACCT GGCAGTTGCA 
GCACATGGAA TCACAGTGTT GACTAGCATT GCTGCTTTCT GCCCTGAGAA AGAGGTCATT 
CAGCTGGAAC AGGAAACAGT GATGGGGATG GAGTCTCTCA TTCTGCTCTG CAGTCAAGAT 
GACAGTAAGA CTGCCCAGGC CACACTAAAA ACGGCGCTCA CGTCTCTGGT GCAGATGTTG 
AAGACTTGTC CTCATCTCAG TCAGAGCTCA GTGGAGCTCC TGCTTAGGCA GCTGCATTGC 
GCATGTGACC CTGCTCGGGT GCTCATGTGT CAGGCTCTGG CTGCTATTGC CACACAACAG 
CCAGTGCTGG TAGAGGGAAT GCTGGGAGAC CTGCTGGAGC TCTTCAGGGT GGCAAGTCAC 
AGGACTTCAG AGAAACAGCA GGAGCTTCTG GTGTCTTTGG CAACTGTGTT ATTTGTGGCC 
AGTCAGGCGT CTCTCTCAGC TGAGGTGAAG GCTGTGATCA GACAACAGTT GGAGAATGTG 
GCCAATGGCT GGACCGTGTA CCAAATTGCC AGACAAGCGT CCCGCATGGG CTGCCATGAT 
TTTTCCAGGG AGTTGTACCA GAGTCTTCGC ACCCGTGTGG CCTCCGAACA CTTTTACTTC 
TGGCTTAACA GTCTGATGGA GTTTTCCCAA GCGGAGCAGT GCCTTAGTGG CCTGGAAGAT 
GGAGACTACA GTGCAGCCAT GAGCGCCATC TCTGAAGCAC TGAAATCCTA CCAGAAGGGA 
ATCGCATCTC TCACGGCTGC CAGTACGCCG CTCAGTCCGC TGACATTCCA ATGTGAGTTT 
GTGAAGCTGC GTATAGACAC TCTTCAGGCT CTGTCTCAGC TCATTTGCAC CTGCAACAGT 
CTGAAGACCA GTCCTCCTCC TGCCATCGCA ACCACGATCG CTCTGTCCTC GGGCAGTGAC 
TTGCAGCGCT GCGGACGAAT CTCCACACAG ATGAAGTTTT CCATGGATGA GTTTAGAAGT 
CTCGCAGCTC GCTATGCTGA CCTGTACCAG TCTTCGTTTG ATGCGGACTA TGCCACCCTT 
CGCAATGTTG AACTACAACA GCAGAGTTGC TTACTCGTAT CTTATGTTAT CGAAGCTTTA 
ATAATCGATC CACAAACAGC CAGTTTCCAG GAGTTTGGCA CTCATGGATC AATTCTGGCA 
GAGAGCGAGT ATGAGCTGAG AATGATGGCT GTGTTCAATC ATGTCCTGGA GGAAGTGGAA 
AATCTAAGCA GGAAACACCC TCCTGTTTCT TACCTGCTTG CTCTCTCTCC ATCTCCCCGC 
ACACCCAATG AGCCGATCCC AGTGCAGAAC AACCAGCAGC TGACCTTAAA AGTGGAGGGT 
GTTATTCAGC ACGGCTCCAG TCCGGGACTC TTCCGGAAGA TTCAGGCAGT CTGTCTTAAA 
GTCAGCTCGA CGTTACAGAC AAAACCAGGA TCAGACTTCA AGATTCCTCT GGAATCCAAA 
ACCAACGAGA TTGAACAGAA AGTAGAGCCT CATAACGATT ACTTCAGCAC ACAGTTCCTG 
CTCAACTTCT CCATCCTGGG CACACACCAG GTATCTGTTG AAGCCTCAGT GGTGGACACC 
AGTGGGATTG AATGGAAGAC CGGCCCTAAA AACACTGTTT CGGTGAAGTC TCTGGAAGAC 
CCATATTCCC AGCAGCTTCG GCATCAATTA CAGCAGCAGC AGCAGAACGT CCCGCAGCCT 
GCAGCCCAGA GGAACATCAG CACACGCTTC CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Many genes in fish have species-specific asymmetric rates of molecular evolution.
BMC genomics720(2006 Feb)
Steinke D,Salzburger W,Braasch I,Meyer A

Identification of zebrafish insertional mutants with defects in visual system development and function.
Genetics170(1)245-61(2005 May)
Gross JM,Perkins BD,Amsterdam A,Ega?a A,Darland T,Matsui JI,Sciascia S,Hopkins N,Dowling JE

Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development.
Nature genetics31(2)135-40(2002 Jun)
Golling G,Amsterdam A,Sun Z,Antonelli M,Maldonado E,Chen W,Burgess S,Haldi M,Artzt K,Farrington S,Lin SY,Nissen RM,Hopkins N

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ints7 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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