pak2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pak2a
Entrez Gene ID	266749
Full Name	p21 protein (Cdc42/Rac)-activated kinase 2a
Synonyms	cb422,kinase,pak2,si:dz198m22.1,wu:fb71h01,zgc:91798
General protein information	Preferred Names p21 protein (Cdc42/Rac)-activated kinase 2a Names serine/threonine-protein kinase PAK 2 p21 (CDKN1A)-activated kinase 2 p21 (CDKN1A)-activated kinase 2a p21-activated kinase 2 redhead rhd
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171262.3	XP_005171319.1	serine/threonine-protein kinase PAK 2 isoform X1
XM_005171262.4	XP_005171319.1	serine/threonine-protein kinase PAK 2 isoform X1
XM_005171263.3	XP_005171320.1	serine/threonine-protein kinase PAK 2 isoform X1
NM_001002717.1	NP_001002717.1	serine/threonine-protein kinase PAK 2
XM_005171263.4	XP_005171320.1	serine/threonine-protein kinase PAK 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PAK2	NP_002568.2
	PAK2	XP_001099936.1
Canis lupus familiaris (dog)	PAK2	XP_849432.1
Bos taurus (cattle)	PAK2	NP_001193656.1
Gallus gallus (chicken)	PAK2	XP_004937052.1
Danio rerio (zebrafish)	pak2b	NP_001020627.1
Mus musculus (house mouse)	Pak2	NP_796300.1
Neurospora crassa	NCU03894	XP_957533.1
Xenopus tropicalis (tropical clawed frog)	pak2	XP_002935145.1
Pan troglodytes (chimpanzee)	PAK2	XP_516976.3
Rattus norvegicus (Norway rat)	LOC100910732	XP_003751114.1
Danio rerio (zebrafish)	pak2a	NP_001002717.1
Magnaporthe oryzae (rice blast fungus)	MGG_12821	XP_003717065.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Toxicity and anti-angiogenicity evaluation of Pak1 inhibitor IPA-3 using zebrafish embryo model.
Jagadeeshan S, Sagayaraj RV, Paneerselvan N, Ghouse SS, Malathi R
Cell biology and toxicology33(1)41-56(2017 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Eph-Pak2a signaling regulates branching of the pharyngeal endoderm by inhibiting late-stage epithelial dynamics.
Choe CP, Crump JG
Development (Cambridge, England)142(6)1089-94(2015 Mar)

Use of the zebrafish larvae as a model to study cigarette smoke condensate toxicity.
Ellis LD, Soo EC, Achenbach JC, Morash MG, Soanes KH
PloS one9(12)e115305(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EphrinB ligands B2a and B3b, the Ephb4a receptor and the Pak2a kinase are required for the development of pharyngeal pouches and the segmentation of the posterior facial skeleton.
Title: Eph-Pak2a signaling regulates branching of the pharyngeal endoderm by inhibiting late-stage epithelial dynamics.

Essential in vivo role for betaPix and Pak2a during embryonic development and a previously unrecognized pathway specifically involved in cerebrovascular stabilization. [BetaPix AND Pak2a]
Title: A betaPix Pak2a signaling pathway regulates cerebral vascular stability in zebrafish.

Data provide evidence for a critical function of Pak2 in vascular integrity and demonstrate a severe disease phenotype resulting from loss of Pak2 function.
Title: pak2a mutations cause cerebral hemorrhage in redhead zebrafish.

The following pak2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pak2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02712	NM_001002717.1 Latest version!	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa02712	XM_005171262.4 Latest version!	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa02712	XM_005171263.4 Latest version!	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa02712	XM_005171262.3	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa02712	XM_005171263.3	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pak2a ( NM_001002717.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002717.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), mRNA.

Price & Availability↑

CloneID	ODa02712
Clone ID Related Accession (Same CDS sequence)	XM_005171262.3 , XM_005171262.4 , XM_005171263.3 , NM_001002717.1 , XM_005171263.4
Accession Version	NM_001002717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase PAK 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076511.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC076511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002717.1
CDS	120..1673
Translation	MSDNGELEDKPPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLKFYDSTGNSRQKYLSFTDKDAPQAKKGSEQSPVKDPDDDDEDAPPPVVAPRPQHTISVYTRSVIDPIPAPAAIADTDGSKAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR

Target ORF information:

RefSeq Version	NM_001002717.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002717.1

ORF Insert Sequence:

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pak2a ( XM_005171262.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171319.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa02712
Clone ID Related Accession (Same CDS sequence)	XM_005171262.3 , XM_005171262.4 , XM_005171263.3 , NM_001002717.1 , XM_005171263.4
Accession Version	XM_005171262.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase PAK 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171262.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171319.1
CDS	450..2003
Translation	MSDNGELEDKPPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLKFYDSTGNSRQKYLSFTDKDAPQAKKGSEQSPVKDPDDDDEDAPPPVVAPRPQHTISVYTRSVIDPIPAPAAIADTDGSKAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR

Target ORF information:

RefSeq Version	XM_005171262.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171262.3

ORF Insert Sequence:

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pak2a ( XM_005171262.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171319.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa02712
Clone ID Related Accession (Same CDS sequence)	XM_005171262.3 , XM_005171262.4 , XM_005171263.3 , NM_001002717.1 , XM_005171263.4
Accession Version	XM_005171262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase PAK 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171262.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171319.1
CDS	443..1996
Translation	MSDNGELEDKPPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLKFYDSTGNSRQKYLSFTDKDAPQAKKGSEQSPVKDPDDDDEDAPPPVVAPRPQHTISVYTRSVIDPIPAPAAIADTDGSKAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR

Target ORF information:

RefSeq Version	XM_005171262.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171262.4

ORF Insert Sequence:

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pak2a ( XM_005171263.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171320.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa02712
Clone ID Related Accession (Same CDS sequence)	XM_005171262.3 , XM_005171262.4 , XM_005171263.3 , NM_001002717.1 , XM_005171263.4
Accession Version	XM_005171263.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase PAK 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171320.1
CDS	469..2022
Translation	MSDNGELEDKPPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLKFYDSTGNSRQKYLSFTDKDAPQAKKGSEQSPVKDPDDDDEDAPPPVVAPRPQHTISVYTRSVIDPIPAPAAIADTDGSKAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR

Target ORF information:

RefSeq Version	XM_005171263.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171263.3

ORF Insert Sequence:

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pak2a ( XM_005171263.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171320.1 Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa02712
Clone ID Related Accession (Same CDS sequence)	XM_005171262.3 , XM_005171262.4 , XM_005171263.3 , NM_001002717.1 , XM_005171263.4
Accession Version	XM_005171263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase PAK 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171263.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171320.1
CDS	444..1997
Translation	MSDNGELEDKPPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLKFYDSTGNSRQKYLSFTDKDAPQAKKGSEQSPVKDPDDDDEDAPPPVVAPRPQHTISVYTRSVIDPIPAPAAIADTDGSKAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR

Target ORF information:

RefSeq Version	XM_005171263.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio p21 protein (Cdc42/Rac)-activated kinase 2a (pak2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171263.4

ORF Insert Sequence:

ATGTCTGACA ACGGAGAGCT GGAGGACAAA CCCCCTGCCC CCCCAGTCAG AATGAGCAGC 
ACCTTCAGCA CCGGTATTAA AGACAGTATG TCAACAAACC CCAGCTCTAA ACCCCTGCCC 
TCCGTCCCAG AGGAGAAGAG GGACAAACCG CGCAACAAGA TCATATCAAT ATTCTCAGCT 
GAGAAAGGAA GAAAAAAAGA CAAGGATAAG GAGCGTCCGG AGATTTCTAA CCCTTCAGAT 
TTCGAGCACA CTATACATGT GGGCTTTGAT TCTGTCACAG GGGAGTTCAC GGGCATGCCA 
GAGCAGTGGG CTCGGCTACT GCAGACCTCC AACATCACGA AATCTGAGCA GAAGAAAAAC 
CCTCAGGCTG TGTTGGACGT GCTCAAATTC TACGACTCCA CAGGCAACAG CAGGCAGAAA 
TACCTCAGCT TCACAGATAA AGATGCACCA CAAGCAAAAA AAGGCTCAGA GCAATCTCCT 
GTCAAAGACC CTGATGATGA TGACGAGGAT GCTCCACCCC CTGTTGTAGC ACCACGCCCA 
CAGCACACCA TATCTGTATA CACTCGTTCT GTCATCGATC CTATTCCAGC ACCTGCTGCC 
ATTGCAGACA CAGATGGCTC TAAAGCTGCA GACAAACAGA AGAAGGGCAA GGGAAAGATG 
ACCGATGAGG AGATTATGGA GAAACTTAGA ACCATTGTCA GTATTGGAGA CCCCAAGAAG 
AAATACACAA GATACGAAAA AATTGGACAA GGTGCGTCTG GTACAGTGTA CACAGCCATT 
GATGTTGCTA CTGGCCAAGA GGTTGCTATT AAGCAGATTA ACCTACAGAA GCAGCCCAAA 
AAAGAGCTGA TCATCAATGA GATCCTGGTG ATGAAGGAGC TGAAGAATCC AAACATTGTC 
AACTTCTTAG ACAGCTTCTT GGTTGGAGAT GAGCTCTTTG TGGTGATGGA GTATCTTGCT 
GGAGGCTCTC TGACAGACGT AGTAACAGAA ACCTGCATGG ATGAGGCTCA AATTGCTGCT 
GTCTGCAGAG AGTGTTTACA AGCACTGGAG TTTCTGCATG CAAACCAGGT CATCCATCGA 
GACATCAAAA GTGACAACGT TCTTTTAGGA ATGGACGGAT CAGTCAAACT AACCGATTTT 
GGGTTCTGTG CTCAAATCAC TCCAGAGCAA AGCAAGAGGA GCACCATGGT GGGAACACCC 
TACTGGATGG CCCCTGAGGT GGTCACACGT AAAGCCTACG GGCCCAAAGT GGACATTTGG 
TCTTTGGGTA TCATGGCCAT TGAAATGGTT GAGGGCGAAC CTCCATATCT CAATGAAAAT 
CCTCTGAGGG CGTTGTACCT CATCGCTACT AATGGCACCC CGGAGCTCCA GAACCCAGAA 
AAGCTGTCTC CCATTTTTAG AGACTTCCTA AACCGCTGCC TGGAGATGGA TGTGGAGAAG 
AGAGGAGGAG GAAAAGAGCT TCTGCAACAT CCTTTCCTCA AGCTGGCAAA GCCTCTTTCC 
AGCCTCACTC CTCTTATACT TGCTGCCAAG GAGGCAATGA AGAGTAACCG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Toxicity and anti-angiogenicity evaluation of Pak1 inhibitor IPA-3 using zebrafish embryo model.
Cell biology and toxicology33(1)41-56(2017 Feb)
Jagadeeshan S,Sagayaraj RV,Paneerselvan N,Ghouse SS,Malathi R

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Eph-Pak2a signaling regulates branching of the pharyngeal endoderm by inhibiting late-stage epithelial dynamics.
Development (Cambridge, England)142(6)1089-94(2015 Mar)
Choe CP,Crump JG

Use of the zebrafish larvae as a model to study cigarette smoke condensate toxicity.
PloS one9(12)e115305(2014)
Ellis LD,Soo EC,Achenbach JC,Morash MG,Soanes KH

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pak2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム