ache cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ache
Entrez Gene ID	114549
Full Name	acetylcholinesterase
Synonyms	zgc:92550
General protein information	Preferred Names acetylcholinesterase Names acetylcholinesterase ziehharmonika zim
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131846.2	NP_571921.1	acetylcholinesterase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACHE	XP_005621080.1
Caenorhabditis elegans (roundworm)	ace-1	NP_510660.1
Bos taurus (cattle)	ACHE	NP_001069688.1
Magnaporthe oryzae (rice blast fungus)	MGG_02130	XP_003708907.1
Homo sapiens (human)	ACHE	NP_000656.1
	ACHE	NP_001121560.1
Mus musculus (house mouse)	Ache	NP_033729.1
Danio rerio (zebrafish)	ache	NP_571921.1
Rattus norvegicus (Norway rat)	Ache	NP_742006.1
Anopheles gambiae (African malaria mosquito)	ACES_ANOGA	XP_321792.2
Pan troglodytes (chimpanzee)	ACHE	XP_003318708.1
Gallus gallus (chicken)	ACHE	NP_990749.1
Xenopus tropicalis (tropical clawed frog)	LOC100490673	XP_002940088.1

Gene Ontology

The following ache gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ache cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98538	NM_131846.2 Latest version!	Danio rerio acetylcholinesterase (ache), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ache ( NM_131846.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571921.1 Danio rerio acetylcholinesterase (ache), mRNA.

Price & Availability↑

CloneID	ODa98538
Clone ID Related Accession (Same CDS sequence)	NM_131846.2
Accession Version	NM_131846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	acetylcholinesterase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC163891.1, BX470232.17, CT027989.8 and EH603490.1. On Oct 1, 2016 this sequence version replaced NM_131846.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC163891.1, GDQH01004936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476760, SAMEA4476797 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGACCT CAGATATTTT GCTCTTGCCC ACTGTGCTAC TCACGTTCCT CTTCCATAAC 
TGTTTCGCAC AGGCTGAACC TGACCTCGTA GTAGCAACAA GATTGGGCAG AGTGCAGGGT 
ACAAGATTGC CTGTTCCAGA CCGGAGTCAT GTGATTGCTT TCTTAGGCAT CCCATATGCT 
GAACCACCCA TTGGGAAGAG GCGTTTCAAG AGAGCTGAGC CCAAAAAGCC CTGGAATAAT 
GTCTTTGAGG CTAAAGAGTT CTCAAATGCC TGCTATCAGT TTGTGGATAC CTCTTACCCG 
GGCTTTCCAG GTATTGAAAT GTGGAACCCC AACAGAGTGA TGAGTGAAGA CTGTCTTTAT 
CTGAATGTGT GGGTACCTCC TACGCCCAGA CCACAGAACC TCACAGTTAT GGTCTGGATA 
TATGGTGGTG GCTTCTATAG TGGCTCTTCC TCTCTTGATG TCTATGATGG CCGTTACCTG 
GCTTACACTG AAAAGGTAGT GGTTGTGTCT ATGAACTATC GTGTTGGAGC TTTTGGATTC 
CTTGCTCTTA ATGGCTCATC AGACGCACCT GGAAACGTGG GTCTCTATGA CCAGAGATTG 
GCCCTCCAGT GGGTACAAGA AAACATCCAC TTCTTTGGCG GTAACCCAAA ACAGGTCACC 
ATTTTTGGAG AAAGTGCTGG GGCAGCATCA GTTGGCATGC ATGTTCTGTC ACCAGACAGC 
CGCCCCCTCT TCACACGTGC CATATTGCAG AGTGGTGTTC CAAATACACC CTGGGCCACT 
GTTACCTTTG ATGAGGCCCG TCGGAGAACC ACCAAGTTGG GCAAACTGGT TGGTTGCACC 
TGGGGCAATG ATACTGAACT GATTGATTGT CTGAGGAATA AACATCCACA GGAACTAATT 
GATCAAGAGT GGCAAGTTCT TCCCTGGAGC AGCCTTTTCC GCTTTTCCTT TGTGCCTGTC 
GTCGATGGGG TTTTCTTTCC CGACACCCCA GATGCAATGA TTAGCTCTGG GAACTTCAAA 
TACACTCAGA TCCTGTTGGG CGTAAATCAG GATGAGGGAT CTTACTTCTT GCTTTATGGG 
GCACCAGGTT TCAGTAAAGA CAATGAGAGT TTAATATCTC GGGAAGACTT CCTGGAAAGT 
GTGAAGATGG GTGTTCCACA TGCCAATGAC ATTGGCTTGG AAGCAGTTAT CCTTCAATAC 
ACTGATTGGA TGGATGAGAA CAACGGCCAA AAGAATAGAG ATGCCATGGA CGATATTGTA 
GGAGATCAAA ATGTCATCTG TCCTCTACAG CATTTTGCAA AGTCATACGC ACAATACGCT 
GCCCTCCATG CCCAGTCATC AGCGGCAGCT CCAGGAACAC TAGGCTGGGG AAACTCAGGA 
CCAACAGGCT ACAACAGTGG CAACTCGCAT GGTGCTGTGT ACCTTTATCT GTTTGACCAT 
CGAGCCTCAA ACCTGGCCTG GCCTGAGTGG ATGGGCGTCA TCCACGGCTA TGAGATTGAG 
TTTGTATTTG GCCTGCCATT GGAGAAGCGA CTCAATTACA CAGCAGAGGA GGAGAAACTC 
AGTCGACGAA TAATGAGATA CTGGGCTAAT TTCGCCCGCA CTGGTAATCC CAATGTTAAT 
ACTGATGGCA CCATGGACAG TCGGAGACGA TGGCCTCAAT TTTCAGCTAA TGAGCAAAAG 
CATGTGGGCT TGAACACAGA GCCAATGAAG GTGCACAAGG GTCTAAGAAC ACAGTTCTGT 
GCTCTGTGGA ACCGCTTCCT GCCTCGTCTG CTTAACATCA CAGATAACAT TGATGATGTT 
GAGCGACAGT GGAAAGTGGA GTTTCATCGC TGGAGCTCTT ACATGATGCA CTGGAAGAGC 
CAGTTCGACC ATTACAGCAA GCAGGAGCGC TGCACAGATC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571921.1
CDS	269..2173
Translation	MKTSDILLLPTVLLTFLFHNCFAQAEPDLVVATRLGRVQGTRLPVPDRSHVIAFLGIPYAEPPIGKRRFKRAEPKKPWNNVFEAKEFSNACYQFVDTSYPGFPGIEMWNPNRVMSEDCLYLNVWVPPTPRPQNLTVMVWIYGGGFYSGSSSLDVYDGRYLAYTEKVVVVSMNYRVGAFGFLALNGSSDAPGNVGLYDQRLALQWVQENIHFFGGNPKQVTIFGESAGAASVGMHVLSPDSRPLFTRAILQSGVPNTPWATVTFDEARRRTTKLGKLVGCTWGNDTELIDCLRNKHPQELIDQEWQVLPWSSLFRFSFVPVVDGVFFPDTPDAMISSGNFKYTQILLGVNQDEGSYFLLYGAPGFSKDNESLISREDFLESVKMGVPHANDIGLEAVILQYTDWMDENNGQKNRDAMDDIVGDQNVICPLQHFAKSYAQYAALHAQSSAAAPGTLGWGNSGPTGYNSGNSHGAVYLYLFDHRASNLAWPEWMGVIHGYEIEFVFGLPLEKRLNYTAEEEKLSRRIMRYWANFARTGNPNVNTDGTMDSRRRWPQFSANEQKHVGLNTEPMKVHKGLRTQFCALWNRFLPRLLNITDNIDDVERQWKVEFHRWSSYMMHWKSQFDHYSKQERCTDL

Target ORF information:

RefSeq Version	NM_131846.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetylcholinesterase (ache), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131846.2

ORF Insert Sequence:

ATGAAGACCT CAGATATTTT GCTCTTGCCC ACTGTGCTAC TCACGTTCCT CTTCCATAAC 
TGTTTCGCAC AGGCTGAACC TGACCTCGTA GTAGCAACAA GATTGGGCAG AGTGCAGGGT 
ACAAGATTGC CTGTTCCAGA CCGGAGTCAT GTGATTGCTT TCTTAGGCAT CCCATATGCT 
GAACCACCCA TTGGGAAGAG GCGTTTCAAG AGAGCTGAGC CCAAAAAGCC CTGGAATAAT 
GTCTTTGAGG CTAAAGAGTT CTCAAATGCC TGCTATCAGT TTGTGGATAC CTCTTACCCG 
GGCTTTCCAG GTATTGAAAT GTGGAACCCC AACAGAGTGA TGAGTGAAGA CTGTCTTTAT 
CTGAATGTGT GGGTACCTCC TACGCCCAGA CCACAGAACC TCACAGTTAT GGTCTGGATA 
TATGGTGGTG GCTTCTATAG TGGCTCTTCC TCTCTTGATG TCTATGATGG CCGTTACCTG 
GCTTACACTG AAAAGGTAGT GGTTGTGTCT ATGAACTATC GTGTTGGAGC TTTTGGATTC 
CTTGCTCTTA ATGGCTCATC AGACGCACCT GGAAACGTGG GTCTCTATGA CCAGAGATTG 
GCCCTCCAGT GGGTACAAGA AAACATCCAC TTCTTTGGCG GTAACCCAAA ACAGGTCACC 
ATTTTTGGAG AAAGTGCTGG GGCAGCATCA GTTGGCATGC ATGTTCTGTC ACCAGACAGC 
CGCCCCCTCT TCACACGTGC CATATTGCAG AGTGGTGTTC CAAATACACC CTGGGCCACT 
GTTACCTTTG ATGAGGCCCG TCGGAGAACC ACCAAGTTGG GCAAACTGGT TGGTTGCACC 
TGGGGCAATG ATACTGAACT GATTGATTGT CTGAGGAATA AACATCCACA GGAACTAATT 
GATCAAGAGT GGCAAGTTCT TCCCTGGAGC AGCCTTTTCC GCTTTTCCTT TGTGCCTGTC 
GTCGATGGGG TTTTCTTTCC CGACACCCCA GATGCAATGA TTAGCTCTGG GAACTTCAAA 
TACACTCAGA TCCTGTTGGG CGTAAATCAG GATGAGGGAT CTTACTTCTT GCTTTATGGG 
GCACCAGGTT TCAGTAAAGA CAATGAGAGT TTAATATCTC GGGAAGACTT CCTGGAAAGT 
GTGAAGATGG GTGTTCCACA TGCCAATGAC ATTGGCTTGG AAGCAGTTAT CCTTCAATAC 
ACTGATTGGA TGGATGAGAA CAACGGCCAA AAGAATAGAG ATGCCATGGA CGATATTGTA 
GGAGATCAAA ATGTCATCTG TCCTCTACAG CATTTTGCAA AGTCATACGC ACAATACGCT 
GCCCTCCATG CCCAGTCATC AGCGGCAGCT CCAGGAACAC TAGGCTGGGG AAACTCAGGA 
CCAACAGGCT ACAACAGTGG CAACTCGCAT GGTGCTGTGT ACCTTTATCT GTTTGACCAT 
CGAGCCTCAA ACCTGGCCTG GCCTGAGTGG ATGGGCGTCA TCCACGGCTA TGAGATTGAG 
TTTGTATTTG GCCTGCCATT GGAGAAGCGA CTCAATTACA CAGCAGAGGA GGAGAAACTC 
AGTCGACGAA TAATGAGATA CTGGGCTAAT TTCGCCCGCA CTGGTAATCC CAATGTTAAT 
ACTGATGGCA CCATGGACAG TCGGAGACGA TGGCCTCAAT TTTCAGCTAA TGAGCAAAAG 
CATGTGGGCT TGAACACAGA GCCAATGAAG GTGCACAAGG GTCTAAGAAC ACAGTTCTGT 
GCTCTGTGGA ACCGCTTCCT GCCTCGTCTG CTTAACATCA CAGATAACAT TGATGATGTT 
GAGCGACAGT GGAAAGTGGA GTTTCATCGC TGGAGCTCTT ACATGATGCA CTGGAAGAGC 
CAGTTCGACC ATTACAGCAA GCAGGAGCGC TGCACAGATC TCTAG