Ache cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ache
Entrez Gene ID	83817
Full Name	acetylcholinesterase
General protein information	Preferred Names acetylcholinesterase Names acetylcholinesterase glycolipid-anchored form of acetylcholinesterase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	catalyzes the hydrolysis of acetylcholine to choline and acetate; plays a role in neuronal development and synapse formation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172009.1	NP_742006.1	acetylcholinesterase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACHE	XP_005621080.1
Caenorhabditis elegans (roundworm)	ace-1	NP_510660.1
Bos taurus (cattle)	ACHE	NP_001069688.1
Magnaporthe oryzae (rice blast fungus)	MGG_02130	XP_003708907.1
Homo sapiens (human)	ACHE	NP_000656.1
	ACHE	NP_001121560.1
Mus musculus (house mouse)	Ache	NP_033729.1
Danio rerio (zebrafish)	ache	NP_571921.1
Rattus norvegicus (Norway rat)	Ache	NP_742006.1
Anopheles gambiae (African malaria mosquito)	ACES_ANOGA	XP_321792.2
Pan troglodytes (chimpanzee)	ACHE	XP_003318708.1
Gallus gallus (chicken)	ACHE	NP_990749.1
Xenopus tropicalis (tropical clawed frog)	LOC100490673	XP_002940088.1

Gene Ontology
Bibliography

Related articles in PubMed

Sepsis decreases the activity of acetylcholinesterase by reducing its expression at the neuromuscular junction.
Wu J, Jin T, Wang H, Li ST
Molecular medicine reports16(4)5263-5268(2017 Oct)

Primary hippocampal neuronal cell death induction after acute and repeated paraquat exposures mediated by AChE variants alteration and cholinergic and glutamatergic transmission disruption.
Del Pino J, Moyano P, D?az GG, Anadon MJ, Diaz MJ, Garc?a JM, Lobo M, Pelayo A, Sola E, Frejo MT
Toxicology39088-99(2017 09)

Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.
Maciel RM, Carvalho FB, Olabiyi AA, Schmatz R, Gutierres JM, Stefanello N, Zanini D, Rosa MM, Andrade CM, Rubin MA, Schetinger MR, Morsch VM, Danesi CC, Lopes STA
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie84559-568(2016 Dec)

Flavonoids induce the expression of acetylcholinesterase in cultured osteoblasts.
Xu ML, Bi CW, Kong AY, Dong TT, Wong YH, Tsim KW
Chemico-biological interactions259(Pt B)295-300(2016 Nov)

Intracerebroventricular D-galactose administration impairs memory and alters activity and expression of acetylcholinesterase in the rat.
Rodrigues AF, Biasibetti H, Zanotto BS, Sanches EF, Pierozan P, Schmitz F, Parisi MM, Barb?-Tuana F, Netto CA, Wyse AT
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience501-6(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Paraquat-induced death of hippocampal neurons was partially mediated by AChE variants inducing and cholinergic and gultamatergic transmission disruption.
Title: Primary hippocampal neuronal cell death induction after acute and repeated paraquat exposures mediated by AChE variants alteration and cholinergic and glutamatergic transmission disruption.

Quercetin improves memory and anxiogenic-like behavior in diabetic rats by preventing the increase in Ache activity seen in streptozocin induced diabetes.
Title: Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.

the induction of PRiMA-linked AChE in osteoblast should be independent to classical estrogen signaling pathway.
Title: Flavonoids induce the expression of acetylcholinesterase in cultured osteoblasts.

This study showed that changes in AChE may, at least in part, to lead to memory impairment caused by galactose. Taken together, our results can help understand the etiopathology of classical galactosemia.
Title: Intracerebroventricular D-galactose administration impairs memory and alters activity and expression of acetylcholinesterase in the rat.

Hindlimb unloading (simulation of hypogravity) leads to an increase in functional activity of acetylcholinesterase on the background of reduced postsynaptic membrane area occupied by acetylcholine receptors.
Title: [Influence of modeling of gravitational unloading on the postsynaptic acetylcholine receptor organization and acetylcholinesterase activity in neuromuscular synapses of rat fast and slow muscles].

The following Ache gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ache cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12716	NM_172009.1 Latest version!	Rattus norvegicus acetylcholinesterase (Ache), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ache ( NM_172009.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742006.1 Rattus norvegicus acetylcholinesterase (Ache), mRNA.

Price & Availability↑

CloneID	ORa12716
Clone ID Related Accession (Same CDS sequence)	NM_172009.1
Accession Version	NM_172009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	acetylcholinesterase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S50879.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642607 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCCTC CCTGGTATCC CCTGCATACA CCCTCCCTGG CTTCTCCACT CCTCTTCCTC 
CTCCTCTCCC TCCTGGGAGG AGGGGCAAGG GCTGAGGGCC GGGAAGACCC TCAGCTGCTG 
GTGAGGGTTC GAGGGGGCCA GCTGAGGGGC ATCCGCCTGA AGGCCCCTGG AGGCCCAGTC 
TCAGCTTTTC TGGGCATCCC CTTTGCAGAG CCACCTGTGG GCTCACGTAG ATTTATGCCA 
CCAGAGCCCA AGCGCCCCTG GTCAGGAATA TTGGATGCTA CCACCTTCCA AAATGTCTGC 
TACCAATACG TGGACACCCT GTACCCTGGG TTTGAGGGTA CCGAGATGTG GAACCCCAAT 
CGAGAGCTGA GTGAAGACTG CCTTTATCTT AATGTGTGGA CACCATACCC CAGGCCTACT 
TCTCCCACAC CTGTCCTCAT CTGGATCTAT GGGGGTGGTT TCTACAGTGG AGCATCCTCC 
TTGGACGTGT ATGACGGCCG TTTCCTGGCC CAGGTTGAGG GAACCGTGTT GGTATCTATG 
AACTACCGAG TGGGAACCTT TGGCTTCTTG GCTCTACCAG GAAGCAGAGA AGCCCCTGGC 
AATGTAGGCC TGCTGGATCA ACGGCTTGCC TTGCAATGGG TACAAGAAAA TATCGCAGCC 
TTTGGGGGAG ACCCAATGTC AGTGACTCTG TTTGGGGAGA GTGCAGGTGC AGCCTCAGTG 
GGCATGCACA TTCTGTCCCT GCCCAGCAGG AGCCTCTTCC ACAGGGCTGT CCTGCAGAGT 
GGCACACCCA ATGGGCCCTG GGCCACTGTG AGTGCGGGAG AGGCCAGGCG CAGGGCCACA 
CTGCTGGCCC GCCTTGTGGG CTGTCCCCCA GGTGGCGCTG GTGGCAATGA CACCGAGCTG 
ATATCCTGCT TGAGGACAAG GCCCGCTCAG GACCTGGTGG ACCACGAGTG GCATGTGCTG 
CCTCAAGAAA GTATCTTCCG GTTTTCCTTC GTGCCTGTGG TGGACGGGGA TTTCCTCAGT 
GACACGCCGG ACGCTCTCAT CAATACTGGA GATTTTCAAG ACCTGCAGGT GCTGGTGGGT 
GTGGTGAAGG ACGAGGGCTC CTACTTTCTG GTTTACGGGG TCCCAGGCTT CAGCAAAGAC 
AATGAATCTC TCATCAGCCG GGCCCAGTTC CTGGCTGGGG TGCGGATCGG TGTACCCCAA 
GCGAGTGACC TGGCGGCCGA GGCTGTGGTC CTGCATTATA CAGACTGGCT GCACCCTGAG 
GACCCTGCCC ACCTGAGAGA TGCCATGAGT GCGGTGGTAG GCGACCACAA CGTTGTGTGC 
CCTGTGGCCC AGCTGGCTGG GCGACTGGCT GCCCAAGGGG CTCGGGTCTA TGCCTACATC 
TTTGAACACC GTGCCTCCAC ATTGACTTGG CCCCTCTGGA TGGGGGTGCC CCATGGCTAT 
GAAATCGAGT TCATCTTTGG GCTCCCCCTG GATCCCTCAC TGAACTACAC CGTGGAGGAG 
AGAATCTTTG CTCAGCGACT TATGCAATAC TGGACCAATT TTGCCCGCAC AGGGGACCCC 
AATGACCCTC GAGACTCTAA GTCTCCACGG TGGCCACCGT ACACCACTGC CGCGCAGCAA 
TACGTGAGCC TGAACCTGAA GCCTTTGGAG GTGCGGCGGG GACTGCGCGC CCAGACCTGC 
GCCTTCTGGA ATCGTTTTCT CCCCAAATTG CTCAGCGCCA CAGACACGCT GGACGAGGCG 
GAGCGCCAGT GGAAGGCCGA GTTCCACCGC TGGAGCTCCT ACATGGTGCA CTGGAAGAAC 
CAGTTCGACC ACTATAGCAA GCAGGAACGC TGCTCAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742006.1
CDS	1..1845
Translation	MRPPWYPLHTPSLASPLLFLLLSLLGGGARAEGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGILDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLIWIYGGGFYSGASSLDVYDGRFLAQVEGTVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELISCLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPDALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPAHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTVEERIFAQRLMQYWTNFARTGDPNDPRDSKSPRWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL

Target ORF information:

RefSeq Version	NM_172009.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acetylcholinesterase (Ache), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172009.1

ORF Insert Sequence:

ATGAGGCCTC CCTGGTATCC CCTGCATACA CCCTCCCTGG CTTCTCCACT CCTCTTCCTC 
CTCCTCTCCC TCCTGGGAGG AGGGGCAAGG GCTGAGGGCC GGGAAGACCC TCAGCTGCTG 
GTGAGGGTTC GAGGGGGCCA GCTGAGGGGC ATCCGCCTGA AGGCCCCTGG AGGCCCAGTC 
TCAGCTTTTC TGGGCATCCC CTTTGCAGAG CCACCTGTGG GCTCACGTAG ATTTATGCCA 
CCAGAGCCCA AGCGCCCCTG GTCAGGAATA TTGGATGCTA CCACCTTCCA AAATGTCTGC 
TACCAATACG TGGACACCCT GTACCCTGGG TTTGAGGGTA CCGAGATGTG GAACCCCAAT 
CGAGAGCTGA GTGAAGACTG CCTTTATCTT AATGTGTGGA CACCATACCC CAGGCCTACT 
TCTCCCACAC CTGTCCTCAT CTGGATCTAT GGGGGTGGTT TCTACAGTGG AGCATCCTCC 
TTGGACGTGT ATGACGGCCG TTTCCTGGCC CAGGTTGAGG GAACCGTGTT GGTATCTATG 
AACTACCGAG TGGGAACCTT TGGCTTCTTG GCTCTACCAG GAAGCAGAGA AGCCCCTGGC 
AATGTAGGCC TGCTGGATCA ACGGCTTGCC TTGCAATGGG TACAAGAAAA TATCGCAGCC 
TTTGGGGGAG ACCCAATGTC AGTGACTCTG TTTGGGGAGA GTGCAGGTGC AGCCTCAGTG 
GGCATGCACA TTCTGTCCCT GCCCAGCAGG AGCCTCTTCC ACAGGGCTGT CCTGCAGAGT 
GGCACACCCA ATGGGCCCTG GGCCACTGTG AGTGCGGGAG AGGCCAGGCG CAGGGCCACA 
CTGCTGGCCC GCCTTGTGGG CTGTCCCCCA GGTGGCGCTG GTGGCAATGA CACCGAGCTG 
ATATCCTGCT TGAGGACAAG GCCCGCTCAG GACCTGGTGG ACCACGAGTG GCATGTGCTG 
CCTCAAGAAA GTATCTTCCG GTTTTCCTTC GTGCCTGTGG TGGACGGGGA TTTCCTCAGT 
GACACGCCGG ACGCTCTCAT CAATACTGGA GATTTTCAAG ACCTGCAGGT GCTGGTGGGT 
GTGGTGAAGG ACGAGGGCTC CTACTTTCTG GTTTACGGGG TCCCAGGCTT CAGCAAAGAC 
AATGAATCTC TCATCAGCCG GGCCCAGTTC CTGGCTGGGG TGCGGATCGG TGTACCCCAA 
GCGAGTGACC TGGCGGCCGA GGCTGTGGTC CTGCATTATA CAGACTGGCT GCACCCTGAG 
GACCCTGCCC ACCTGAGAGA TGCCATGAGT GCGGTGGTAG GCGACCACAA CGTTGTGTGC 
CCTGTGGCCC AGCTGGCTGG GCGACTGGCT GCCCAAGGGG CTCGGGTCTA TGCCTACATC 
TTTGAACACC GTGCCTCCAC ATTGACTTGG CCCCTCTGGA TGGGGGTGCC CCATGGCTAT 
GAAATCGAGT TCATCTTTGG GCTCCCCCTG GATCCCTCAC TGAACTACAC CGTGGAGGAG 
AGAATCTTTG CTCAGCGACT TATGCAATAC TGGACCAATT TTGCCCGCAC AGGGGACCCC 
AATGACCCTC GAGACTCTAA GTCTCCACGG TGGCCACCGT ACACCACTGC CGCGCAGCAA 
TACGTGAGCC TGAACCTGAA GCCTTTGGAG GTGCGGCGGG GACTGCGCGC CCAGACCTGC 
GCCTTCTGGA ATCGTTTTCT CCCCAAATTG CTCAGCGCCA CAGACACGCT GGACGAGGCG 
GAGCGCCAGT GGAAGGCCGA GTTCCACCGC TGGAGCTCCT ACATGGTGCA CTGGAAGAAC 
CAGTTCGACC ACTATAGCAA GCAGGAACGC TGCTCAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sepsis decreases the activity of acetylcholinesterase by reducing its expression at the neuromuscular junction.
Molecular medicine reports16(4)5263-5268(2017 Oct)
Wu J,Jin T,Wang H,Li ST

Primary hippocampal neuronal cell death induction after acute and repeated paraquat exposures mediated by AChE variants alteration and cholinergic and glutamatergic transmission disruption.
Toxicology39088-99(2017 09)
Del Pino J,Moyano P,D?az GG,Anadon MJ,Diaz MJ,Garc?a JM,Lobo M,Pelayo A,Sola E,Frejo MT

Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie84559-568(2016 Dec)
Maciel RM,Carvalho FB,Olabiyi AA,Schmatz R,Gutierres JM,Stefanello N,Zanini D,Rosa MM,Andrade CM,Rubin MA,Schetinger MR,Morsch VM,Danesi CC,Lopes STA

Flavonoids induce the expression of acetylcholinesterase in cultured osteoblasts.
Chemico-biological interactions259(Pt B)295-300(2016 Nov)
Xu ML,Bi CW,Kong AY,Dong TT,Wong YH,Tsim KW

Intracerebroventricular D-galactose administration impairs memory and alters activity and expression of acetylcholinesterase in the rat.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience501-6(2016 May)
Rodrigues AF,Biasibetti H,Zanotto BS,Sanches EF,Pierozan P,Schmitz F,Parisi MM,Barb?-Tuana F,Netto CA,Wyse AT

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Ache cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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