blzf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	blzf1
Entrez Gene ID	114452
Full Name	basic leucine zipper nuclear factor 1
Synonyms	Golgin45,fe06a04,wu:fe06a04,zgc:66280,zgc:85871
General protein information	Preferred Names basic leucine zipper nuclear factor 1 Names golgin-45
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009305126.1	XP_009303401.1	golgin-45 isoform X1
XM_021476915.1	XP_021332590.1	golgin-45 isoform X1
NM_001144803.1	NP_001138275.1	golgin-45

Pathways from BioSystems

Homologies

Homo sapiens (human)	BLZF1	NP_003657.1
	BLZF1	XP_001094479.1
Danio rerio (zebrafish)	blzf1	NP_001138275.1
Xenopus tropicalis (tropical clawed frog)	blzf1	NP_001008041.2
Pan troglodytes (chimpanzee)	BLZF1	NP_001233389.1
Canis lupus familiaris (dog)	BLZF1	XP_547475.2
Gallus gallus (chicken)	BLZF1	XP_004938282.1
Bos taurus (cattle)	BLZF1	NP_001039364.1
Mus musculus (house mouse)	Blzf1	NP_001153680.1
Rattus norvegicus (Norway rat)	Blzf1	NP_001017494.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of novel MYO18A interaction partners required for myoblast adhesion and muscle integrity.
Cao JM, Cheng XN, Li SQ, Heller S, Xu ZG, Shi DL
Scientific reports636768(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
Sreenivasan R, Cai M, Bartfai R, Wang X, Christoffels A, Orban L
PloS one3(3)e1791(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following blzf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the blzf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31443	NM_001144803.1 Latest version!	Danio rerio basic leucine zipper nuclear factor 1 (blzf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa31443	XM_009305126.1 Latest version!	Danio rerio basic leucine zipper nuclear factor 1 (blzf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa31443	XM_021476915.1 Latest version!	Danio rerio basic leucine zipper nuclear factor 1 (blzf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

blzf1 ( NM_001144803.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001138275.1 Danio rerio basic leucine zipper nuclear factor 1 (blzf1), mRNA.

Price & Availability↑

CloneID	ODa31443
Clone ID Related Accession (Same CDS sequence)	XM_009305126.1 , XM_021476915.1 , NM_001144803.1
Accession Version	NM_001144803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	golgin-45
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CU571081.7. On or before Oct 1, 2011 this sequence version replaced NM_213006.2, XM_001331677.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01002354.1, GFIL01002352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGTCTG CTGAACTGAT CATTAAAGAT GTGTCAGTGG TGGCCGTGCG GGGCCCTGGC 
GATGGCATGG AGACAGATAA ACCCCTGCCA GCCCCTGAGC AGCTGACGGA GGCCCCGCTC 
CAGAAGATCT GCTCTCCTAA ACACAGCCCG AGAGTGGCCA CCGGGTCTCC AGGAGTGCTG 
CACCTGGGCA AGGTGAGCCG GGACGCCTGT TTGGAGCTGG AGGCAGTGAG GATCGTGGTT 
CCCCGGGCTG CCATCATCCG CACCGGGCGC TCAGGGGCGC ACACTGATGA CAGGGGGTCT 
CCTCAACACG GGGACGAGCC TCCACCTGCT CCACCCTCAC CTCCACCTCC AGCAGAGCCG 
CTGCAGGAGT ACAAGAACAG CCTGGAGAAA CTGCAGAGCT CAGAGAGACG CCTGCTGCAG 
GATAAAGAAG GCCTGTCCAA CCAGCTGAGG GTCCAGACCG AGGTGAACCG TGAGCTGAAG 
AAGCTGCTGG TGGCGTCAGT CGGTGATGAT CTGCAGTATC ACTTTGAGCG CCTGGCGAGA 
GAAAAAAACC ACCTGATCCT GGAGAACGAG ACTCTGTGCC GAAACCTGTC GCACACGGCG 
GAGCAGCTGG AGCGCATGAG CATTCAGTGT GACGTCTGGA GGAGCAAGTT CCTCGCCAGC 
CGTGTGATGG CGGAGGAACT GACCAATGCC AGATCGGCTC TACAGCGGCA GACGCGAGAT 
GCTCAGTGCG CCATTCAGGA CCTGCTGAGT GAAAGAGAAG AGTTTTCCAG AGACATGACG 
CACACGCACA GGTGTCTGGA GCAGCTGCTG GTGTCTCTGC AGTGGGGGCG TCAGCAGACG 
TATTACCCCA GCGCTAAGCC GCTCAGCACT GGAGAACTGG CCACAGCCAA TCACAAGCTG 
GCGGACGCCA TCAACGCTCA CCTGCTGGGC AAACTGGAGA ACAGCGGCGG CAAGAAGAGC 
CCGAGCACTG AGATCTGCAA CACACCGGCC GAGAAGATGG CGGAGAAGGT TCTGCGGAGT 
TTAGACCCGG TCTGCGTCTC CGAGCAGGAA CACGACGCGT CTTCATCTGA TTCGCCGTTT 
CTGCCCAACA GGAAGAGCAT TGGCCGCTTC CACCCGTACA CGCGCTACGA GAACATCACG 
TTTAACTGCT GCGAGCGCTG CCACGGGGAG ATCATCGTTC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138275.1
CDS	136..1320
Translation	MMSAELIIKDVSVVAVRGPGDGMETDKPLPAPEQLTEAPLQKICSPKHSPRVATGSPGVLHLGKVSRDACLELEAVRIVVPRAAIIRTGRSGAHTDDRGSPQHGDEPPPAPPSPPPPAEPLQEYKNSLEKLQSSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNHLILENETLCRNLSHTAEQLERMSIQCDVWRSKFLASRVMAEELTNARSALQRQTRDAQCAIQDLLSEREEFSRDMTHTHRCLEQLLVSLQWGRQQTYYPSAKPLSTGELATANHKLADAINAHLLGKLENSGGKKSPSTEICNTPAEKMAEKVLRSLDPVCVSEQEHDASSSDSPFLPNRKSIGRFHPYTRYENITFNCCERCHGEIIVL

Target ORF information:

RefSeq Version	NM_001144803.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio basic leucine zipper nuclear factor 1 (blzf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144803.1

ORF Insert Sequence:

ATGATGTCTG CTGAACTGAT CATTAAAGAT GTGTCAGTGG TGGCCGTGCG GGGCCCTGGC 
GATGGCATGG AGACAGATAA ACCCCTGCCA GCCCCTGAGC AGCTGACGGA GGCCCCGCTC 
CAGAAGATCT GCTCTCCTAA ACACAGCCCG AGAGTGGCCA CCGGGTCTCC AGGAGTGCTG 
CACCTGGGCA AGGTGAGCCG GGACGCCTGT TTGGAGCTGG AGGCAGTGAG GATCGTGGTT 
CCCCGGGCTG CCATCATCCG CACCGGGCGC TCAGGGGCGC ACACTGATGA CAGGGGGTCT 
CCTCAACACG GGGACGAGCC TCCACCTGCT CCACCCTCAC CTCCACCTCC AGCAGAGCCG 
CTGCAGGAGT ACAAGAACAG CCTGGAGAAA CTGCAGAGCT CAGAGAGACG CCTGCTGCAG 
GATAAAGAAG GCCTGTCCAA CCAGCTGAGG GTCCAGACCG AGGTGAACCG TGAGCTGAAG 
AAGCTGCTGG TGGCGTCAGT CGGTGATGAT CTGCAGTATC ACTTTGAGCG CCTGGCGAGA 
GAAAAAAACC ACCTGATCCT GGAGAACGAG ACTCTGTGCC GAAACCTGTC GCACACGGCG 
GAGCAGCTGG AGCGCATGAG CATTCAGTGT GACGTCTGGA GGAGCAAGTT CCTCGCCAGC 
CGTGTGATGG CGGAGGAACT GACCAATGCC AGATCGGCTC TACAGCGGCA GACGCGAGAT 
GCTCAGTGCG CCATTCAGGA CCTGCTGAGT GAAAGAGAAG AGTTTTCCAG AGACATGACG 
CACACGCACA GGTGTCTGGA GCAGCTGCTG GTGTCTCTGC AGTGGGGGCG TCAGCAGACG 
TATTACCCCA GCGCTAAGCC GCTCAGCACT GGAGAACTGG CCACAGCCAA TCACAAGCTG 
GCGGACGCCA TCAACGCTCA CCTGCTGGGC AAACTGGAGA ACAGCGGCGG CAAGAAGAGC 
CCGAGCACTG AGATCTGCAA CACACCGGCC GAGAAGATGG CGGAGAAGGT TCTGCGGAGT 
TTAGACCCGG TCTGCGTCTC CGAGCAGGAA CACGACGCGT CTTCATCTGA TTCGCCGTTT 
CTGCCCAACA GGAAGAGCAT TGGCCGCTTC CACCCGTACA CGCGCTACGA GAACATCACG 
TTTAACTGCT GCGAGCGCTG CCACGGGGAG ATCATCGTTC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

blzf1 ( XM_009305126.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303401.1 Danio rerio basic leucine zipper nuclear factor 1 (blzf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa31443
Clone ID Related Accession (Same CDS sequence)	XM_009305126.1 , XM_021476915.1 , NM_001144803.1
Accession Version	XM_009305126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	golgin-45 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTG CTGAACTGAT CATTAAAGAT GTGTCAGTGG TGGCCGTGCG GGGCCCTGGC 
GATGGCATGG AGACAGATAA ACCCCTGCCA GCCCCTGAGC AGCTGACGGA GGCCCCGCTC 
CAGAAGATCT GCTCTCCTAA ACACAGCCCG AGAGTGGCCA CCGGGTCTCC AGGAGTGCTG 
CACCTGGGCA AGGTGAGCCG GGACGCCTGT TTGGAGCTGG AGGCAGTGAG GATCGTGGTT 
CCCCGGGCTG CCATCATCCG CACCGGGCGC TCAGGGGCGC ACACTGATGA CAGGGGGTCT 
CCTCAACACG GGGACGAGCC TCCACCTGCT CCACCCTCAC CTCCACCTCC AGCAGAGCCG 
CTGCAGGAGT ACAAGAACAG CCTGGAGAAA CTGCAGAGCT CAGAGAGACG CCTGCTGCAG 
GATAAAGAAG GCCTGTCCAA CCAGCTGAGG GTCCAGACCG AGGTGAACCG TGAGCTGAAG 
AAGCTGCTGG TGGCGTCAGT CGGTGATGAT CTGCAGTATC ACTTTGAGCG CCTGGCGAGA 
GAAAAAAACC ACCTGATCCT GGAGAACGAG ACTCTGTGCC GAAACCTGTC GCACACGGCG 
GAGCAGCTGG AGCGCATGAG CATTCAGTGT GACGTCTGGA GGAGCAAGTT CCTCGCCAGC 
CGTGTGATGG CGGAGGAACT GACCAATGCC AGATCGGCTC TACAGCGGCA GACGCGAGAT 
GCTCAGTGCG CCATTCAGGA CCTGCTGAGT GAAAGAGAAG AGTTTTCCAG AGACATGACG 
CACACGCACA GGTGTCTGGA GCAGCTGCTG GTGTCTCTGC AGTGGGGGCG TCAGCAGACG 
TATTACCCCA GCGCTAAGCC GCTCAGCACT GGAGAACTGG CCACAGCCAA TCACAAGCTG 
GCGGACGCCA TCAACGCTCA CCTGCTGGGC AAACTGGAGA ACAGCGGCGG CAAGAAGAGC 
CCGAGCACTG AGATCTGCAA CACACCGGCC GAGAAGATGG CGGAGAAGGT TCTGCGGAGT 
TTAGACCCGG TCTGCGTCTC CGAGCAGGAA CACGACGCGT CTTCATCTGA TTCGCCGTTT 
CTGCCCAACA GGAAGAGCAT TGGCCGCTTC CACCCGTACA CGCGCTACGA GAACATCACG 
TTTAACTGCT GCGAGCGCTG CCACGGGGAG ATCATCGTTC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303401.1
CDS	191..1375
Translation	MMSAELIIKDVSVVAVRGPGDGMETDKPLPAPEQLTEAPLQKICSPKHSPRVATGSPGVLHLGKVSRDACLELEAVRIVVPRAAIIRTGRSGAHTDDRGSPQHGDEPPPAPPSPPPPAEPLQEYKNSLEKLQSSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNHLILENETLCRNLSHTAEQLERMSIQCDVWRSKFLASRVMAEELTNARSALQRQTRDAQCAIQDLLSEREEFSRDMTHTHRCLEQLLVSLQWGRQQTYYPSAKPLSTGELATANHKLADAINAHLLGKLENSGGKKSPSTEICNTPAEKMAEKVLRSLDPVCVSEQEHDASSSDSPFLPNRKSIGRFHPYTRYENITFNCCERCHGEIIVL

Target ORF information:

RefSeq Version	XM_009305126.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio basic leucine zipper nuclear factor 1 (blzf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305126.1

ORF Insert Sequence:

ATGATGTCTG CTGAACTGAT CATTAAAGAT GTGTCAGTGG TGGCCGTGCG GGGCCCTGGC 
GATGGCATGG AGACAGATAA ACCCCTGCCA GCCCCTGAGC AGCTGACGGA GGCCCCGCTC 
CAGAAGATCT GCTCTCCTAA ACACAGCCCG AGAGTGGCCA CCGGGTCTCC AGGAGTGCTG 
CACCTGGGCA AGGTGAGCCG GGACGCCTGT TTGGAGCTGG AGGCAGTGAG GATCGTGGTT 
CCCCGGGCTG CCATCATCCG CACCGGGCGC TCAGGGGCGC ACACTGATGA CAGGGGGTCT 
CCTCAACACG GGGACGAGCC TCCACCTGCT CCACCCTCAC CTCCACCTCC AGCAGAGCCG 
CTGCAGGAGT ACAAGAACAG CCTGGAGAAA CTGCAGAGCT CAGAGAGACG CCTGCTGCAG 
GATAAAGAAG GCCTGTCCAA CCAGCTGAGG GTCCAGACCG AGGTGAACCG TGAGCTGAAG 
AAGCTGCTGG TGGCGTCAGT CGGTGATGAT CTGCAGTATC ACTTTGAGCG CCTGGCGAGA 
GAAAAAAACC ACCTGATCCT GGAGAACGAG ACTCTGTGCC GAAACCTGTC GCACACGGCG 
GAGCAGCTGG AGCGCATGAG CATTCAGTGT GACGTCTGGA GGAGCAAGTT CCTCGCCAGC 
CGTGTGATGG CGGAGGAACT GACCAATGCC AGATCGGCTC TACAGCGGCA GACGCGAGAT 
GCTCAGTGCG CCATTCAGGA CCTGCTGAGT GAAAGAGAAG AGTTTTCCAG AGACATGACG 
CACACGCACA GGTGTCTGGA GCAGCTGCTG GTGTCTCTGC AGTGGGGGCG TCAGCAGACG 
TATTACCCCA GCGCTAAGCC GCTCAGCACT GGAGAACTGG CCACAGCCAA TCACAAGCTG 
GCGGACGCCA TCAACGCTCA CCTGCTGGGC AAACTGGAGA ACAGCGGCGG CAAGAAGAGC 
CCGAGCACTG AGATCTGCAA CACACCGGCC GAGAAGATGG CGGAGAAGGT TCTGCGGAGT 
TTAGACCCGG TCTGCGTCTC CGAGCAGGAA CACGACGCGT CTTCATCTGA TTCGCCGTTT 
CTGCCCAACA GGAAGAGCAT TGGCCGCTTC CACCCGTACA CGCGCTACGA GAACATCACG 
TTTAACTGCT GCGAGCGCTG CCACGGGGAG ATCATCGTTC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

blzf1 ( XM_021476915.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332590.1 Danio rerio basic leucine zipper nuclear factor 1 (blzf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa31443
Clone ID Related Accession (Same CDS sequence)	XM_009305126.1 , XM_021476915.1 , NM_001144803.1
Accession Version	XM_021476915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	golgin-45 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTG CTGAACTGAT CATTAAAGAT GTGTCAGTGG TGGCCGTGCG GGGCCCTGGC 
GATGGCATGG AGACAGATAA ACCCCTGCCA GCCCCTGAGC AGCTGACGGA GGCCCCGCTC 
CAGAAGATCT GCTCTCCTAA ACACAGCCCG AGAGTGGCCA CCGGGTCTCC AGGAGTGCTG 
CACCTGGGCA AGGTGAGCCG GGACGCCTGT TTGGAGCTGG AGGCAGTGAG GATCGTGGTT 
CCCCGGGCTG CCATCATCCG CACCGGGCGC TCAGGGGCGC ACACTGATGA CAGGGGGTCT 
CCTCAACACG GGGACGAGCC TCCACCTGCT CCACCCTCAC CTCCACCTCC AGCAGAGCCG 
CTGCAGGAGT ACAAGAACAG CCTGGAGAAA CTGCAGAGCT CAGAGAGACG CCTGCTGCAG 
GATAAAGAAG GCCTGTCCAA CCAGCTGAGG GTCCAGACCG AGGTGAACCG TGAGCTGAAG 
AAGCTGCTGG TGGCGTCAGT CGGTGATGAT CTGCAGTATC ACTTTGAGCG CCTGGCGAGA 
GAAAAAAACC ACCTGATCCT GGAGAACGAG ACTCTGTGCC GAAACCTGTC GCACACGGCG 
GAGCAGCTGG AGCGCATGAG CATTCAGTGT GACGTCTGGA GGAGCAAGTT CCTCGCCAGC 
CGTGTGATGG CGGAGGAACT GACCAATGCC AGATCGGCTC TACAGCGGCA GACGCGAGAT 
GCTCAGTGCG CCATTCAGGA CCTGCTGAGT GAAAGAGAAG AGTTTTCCAG AGACATGACG 
CACACGCACA GGTGTCTGGA GCAGCTGCTG GTGTCTCTGC AGTGGGGGCG TCAGCAGACG 
TATTACCCCA GCGCTAAGCC GCTCAGCACT GGAGAACTGG CCACAGCCAA TCACAAGCTG 
GCGGACGCCA TCAACGCTCA CCTGCTGGGC AAACTGGAGA ACAGCGGCGG CAAGAAGAGC 
CCGAGCACTG AGATCTGCAA CACACCGGCC GAGAAGATGG CGGAGAAGGT TCTGCGGAGT 
TTAGACCCGG TCTGCGTCTC CGAGCAGGAA CACGACGCGT CTTCATCTGA TTCGCCGTTT 
CTGCCCAACA GGAAGAGCAT TGGCCGCTTC CACCCGTACA CGCGCTACGA GAACATCACG 
TTTAACTGCT GCGAGCGCTG CCACGGGGAG ATCATCGTTC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332590.1
CDS	173..1357
Translation	MMSAELIIKDVSVVAVRGPGDGMETDKPLPAPEQLTEAPLQKICSPKHSPRVATGSPGVLHLGKVSRDACLELEAVRIVVPRAAIIRTGRSGAHTDDRGSPQHGDEPPPAPPSPPPPAEPLQEYKNSLEKLQSSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNHLILENETLCRNLSHTAEQLERMSIQCDVWRSKFLASRVMAEELTNARSALQRQTRDAQCAIQDLLSEREEFSRDMTHTHRCLEQLLVSLQWGRQQTYYPSAKPLSTGELATANHKLADAINAHLLGKLENSGGKKSPSTEICNTPAEKMAEKVLRSLDPVCVSEQEHDASSSDSPFLPNRKSIGRFHPYTRYENITFNCCERCHGEIIVL

Target ORF information:

RefSeq Version	XM_021476915.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio basic leucine zipper nuclear factor 1 (blzf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476915.1

ORF Insert Sequence:

ATGATGTCTG CTGAACTGAT CATTAAAGAT GTGTCAGTGG TGGCCGTGCG GGGCCCTGGC 
GATGGCATGG AGACAGATAA ACCCCTGCCA GCCCCTGAGC AGCTGACGGA GGCCCCGCTC 
CAGAAGATCT GCTCTCCTAA ACACAGCCCG AGAGTGGCCA CCGGGTCTCC AGGAGTGCTG 
CACCTGGGCA AGGTGAGCCG GGACGCCTGT TTGGAGCTGG AGGCAGTGAG GATCGTGGTT 
CCCCGGGCTG CCATCATCCG CACCGGGCGC TCAGGGGCGC ACACTGATGA CAGGGGGTCT 
CCTCAACACG GGGACGAGCC TCCACCTGCT CCACCCTCAC CTCCACCTCC AGCAGAGCCG 
CTGCAGGAGT ACAAGAACAG CCTGGAGAAA CTGCAGAGCT CAGAGAGACG CCTGCTGCAG 
GATAAAGAAG GCCTGTCCAA CCAGCTGAGG GTCCAGACCG AGGTGAACCG TGAGCTGAAG 
AAGCTGCTGG TGGCGTCAGT CGGTGATGAT CTGCAGTATC ACTTTGAGCG CCTGGCGAGA 
GAAAAAAACC ACCTGATCCT GGAGAACGAG ACTCTGTGCC GAAACCTGTC GCACACGGCG 
GAGCAGCTGG AGCGCATGAG CATTCAGTGT GACGTCTGGA GGAGCAAGTT CCTCGCCAGC 
CGTGTGATGG CGGAGGAACT GACCAATGCC AGATCGGCTC TACAGCGGCA GACGCGAGAT 
GCTCAGTGCG CCATTCAGGA CCTGCTGAGT GAAAGAGAAG AGTTTTCCAG AGACATGACG 
CACACGCACA GGTGTCTGGA GCAGCTGCTG GTGTCTCTGC AGTGGGGGCG TCAGCAGACG 
TATTACCCCA GCGCTAAGCC GCTCAGCACT GGAGAACTGG CCACAGCCAA TCACAAGCTG 
GCGGACGCCA TCAACGCTCA CCTGCTGGGC AAACTGGAGA ACAGCGGCGG CAAGAAGAGC 
CCGAGCACTG AGATCTGCAA CACACCGGCC GAGAAGATGG CGGAGAAGGT TCTGCGGAGT 
TTAGACCCGG TCTGCGTCTC CGAGCAGGAA CACGACGCGT CTTCATCTGA TTCGCCGTTT 
CTGCCCAACA GGAAGAGCAT TGGCCGCTTC CACCCGTACA CGCGCTACGA GAACATCACG 
TTTAACTGCT GCGAGCGCTG CCACGGGGAG ATCATCGTTC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of novel MYO18A interaction partners required for myoblast adhesion and muscle integrity.
Scientific reports636768(2016 Nov)
Cao JM,Cheng XN,Li SQ,Heller S,Xu ZG,Shi DL

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
PloS one3(3)e1791(2008 Mar)
Sreenivasan R,Cai M,Bartfai R,Wang X,Christoffels A,Orban L

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blzf1 cDNA ORF clone, Danio rerio(zebrafish)

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