tyk2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tyk2
Entrez Gene ID	100536675
Full Name	tyrosine kinase 2
General protein information	Preferred Names tyrosine kinase 2 Names non-receptor tyrosine-protein kinase TYK2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003198153.4	XP_003198201.2	non-receptor tyrosine-protein kinase TYK2 isoform X1
XM_005164236.4	XP_005164293.1	non-receptor tyrosine-protein kinase TYK2 isoform X2
XM_009299743.2	XP_009298018.1	non-receptor tyrosine-protein kinase TYK2 isoform X1
XM_009299743.3	XP_009298018.1	non-receptor tyrosine-protein kinase TYK2 isoform X1
XM_005164236.3	XP_005164293.1	non-receptor tyrosine-protein kinase TYK2 isoform X2
XM_003198153.5	XP_003198201.2	non-receptor tyrosine-protein kinase TYK2 isoform X1

Pathways from BioSystems

Homologies

	TYK2	XP_001101130.2
Mus musculus (house mouse)	Tyk2	NP_001192241.1
Pan troglodytes (chimpanzee)	TYK2	XP_001165313.2
Rattus norvegicus (Norway rat)	Tyk2	NP_001244276.1
Homo sapiens (human)	TYK2	NP_003322.3
Canis lupus familiaris (dog)	TYK2	XP_005633269.1
Bos taurus (cattle)	TYK2	NP_001107236.1
Xenopus tropicalis (tropical clawed frog)	tyk2	XP_004918872.1
Danio rerio (zebrafish)	tyk2	XP_003198201.2

Gene Ontology

The following tyk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tyk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08722	XM_009299743.3 Latest version!	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa08722	XM_003198153.5 Latest version!	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa08723	XM_005164236.4 Latest version!	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa08722	XM_009299743.2	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa08723	XM_005164236.3	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa08722	XM_003198153.4	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tyk2 ( XM_003198153.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198201.2 Danio rerio tyrosine kinase 2 (tyk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08722
Clone ID Related Accession (Same CDS sequence)	XM_003198153.4 , XM_009299743.2 , XM_009299743.3 , XM_003198153.5
Accession Version	XM_003198153.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	non-receptor tyrosine-protein kinase TYK2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003198153.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198201.2
CDS	182..3679
Translation	MMYRRGRSRRSKKAGTSKGQSEGPGVHVFLFWTKDGERCLSYKKGEVTAEDLTTEAAKAVGITPVCHALFALYDPASLCWYSPNHNFNAENQSGLLLHFRLRFYFHKWHGLGENAPEVGRYALRTSAGTQQATSHLLELSALEYLFAQAKYDFVNDLVPLNAVSGEEEIGRYQNECLGMAVLHLSYTALQTGSSLQDVAKQTSFLRCIPLSFSRHIAKQNILTRFRIRRVFAHFVNTFQQHTVGVGKLGSQEVMYKYLSTLERLAPHFGVETFPVFHLDLRADGGDSGSYLITSRTQNPPEETINEPTHEIRVSGSDGIWWRKFSAQRSQSDDYSHSQNKETAALNEEEDWNIFCDFPEISLIAIQGINVCISRQDNMSMDISLDSSIQARSLVSLLDGYFRLTTDAHHYLCREVAPPRVVLSESNDLHGPLMDEFVLQKLKREVEEGAFLVRWSALDYHRIILASLSRSKKEGENPVYKQFRIRQNGSLFHLEGWDQDFSSIKELTSSLKTFVLKSGEDSFTLKKCCLPKPGELSNLLIIRKGAKSSIKSASETPDLSKLSFNPIKDKEITKEQHLGRGTRTNIYSGWLKIRGSLEDEDDESNNNHCKTKGIRVVLKILEQSQKDKALAFFETASLMSQVSHCHLIFVHGLSVKGTENIMVEEFVEFGPLDVFLHRERAHVSPQWKFTVAKQLASALCYLETKRLVHGNVCGKNILVVRKGLEEGTSPFVKLSDPGISLSALSQGERVERIPWIAPECVADGTRIGSAADKWSFGATLLEICNNGDLHVSNSTLSEKQRFYETQSRLAVPSSQELASFISRCLTYDAAARPSFRTILRELTEIQIKNPDISSECESLPDKDPSVFYKRYLRKIRDLGEGHFGKVMLYVYDPHNDGTGEYVAVKALKQEGDGSSLHVSWMKEIEILKSLYHNNIVKYKGCCTEMGGQVVQLIMEYVPLGSLREYMPKYGQGIGQGLLFAQQICLGMDYLHSKRYIHRDLAARNVLLENEGVVKIGDFGLTKYIPEGDIYYRVREDGDSPVFWYAIECLKESKFSFASDVWSFAVTLYEILTNCSHRLSPPSKFSDMMGTVHGQMTVVKLISLLEKNERLPCPPGCPREVYSLMEQCWDFNPDQRPSFLSLAESLEEIRGPYKQPSSLQLAQLNHR

Target ORF information:

RefSeq Version	XM_003198153.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198153.4

ORF Insert Sequence:

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tyk2 ( XM_009299743.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298018.1 Danio rerio tyrosine kinase 2 (tyk2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08722
Clone ID Related Accession (Same CDS sequence)	XM_003198153.4 , XM_009299743.2 , XM_009299743.3 , XM_003198153.5
Accession Version	XM_009299743.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	non-receptor tyrosine-protein kinase TYK2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298018.1
CDS	181..3678
Translation	MMYRRGRSRRSKKAGTSKGQSEGPGVHVFLFWTKDGERCLSYKKGEVTAEDLTTEAAKAVGITPVCHALFALYDPASLCWYSPNHNFNAENQSGLLLHFRLRFYFHKWHGLGENAPEVGRYALRTSAGTQQATSHLLELSALEYLFAQAKYDFVNDLVPLNAVSGEEEIGRYQNECLGMAVLHLSYTALQTGSSLQDVAKQTSFLRCIPLSFSRHIAKQNILTRFRIRRVFAHFVNTFQQHTVGVGKLGSQEVMYKYLSTLERLAPHFGVETFPVFHLDLRADGGDSGSYLITSRTQNPPEETINEPTHEIRVSGSDGIWWRKFSAQRSQSDDYSHSQNKETAALNEEEDWNIFCDFPEISLIAIQGINVCISRQDNMSMDISLDSSIQARSLVSLLDGYFRLTTDAHHYLCREVAPPRVVLSESNDLHGPLMDEFVLQKLKREVEEGAFLVRWSALDYHRIILASLSRSKKEGENPVYKQFRIRQNGSLFHLEGWDQDFSSIKELTSSLKTFVLKSGEDSFTLKKCCLPKPGELSNLLIIRKGAKSSIKSASETPDLSKLSFNPIKDKEITKEQHLGRGTRTNIYSGWLKIRGSLEDEDDESNNNHCKTKGIRVVLKILEQSQKDKALAFFETASLMSQVSHCHLIFVHGLSVKGTENIMVEEFVEFGPLDVFLHRERAHVSPQWKFTVAKQLASALCYLETKRLVHGNVCGKNILVVRKGLEEGTSPFVKLSDPGISLSALSQGERVERIPWIAPECVADGTRIGSAADKWSFGATLLEICNNGDLHVSNSTLSEKQRFYETQSRLAVPSSQELASFISRCLTYDAAARPSFRTILRELTEIQIKNPDISSECESLPDKDPSVFYKRYLRKIRDLGEGHFGKVMLYVYDPHNDGTGEYVAVKALKQEGDGSSLHVSWMKEIEILKSLYHNNIVKYKGCCTEMGGQVVQLIMEYVPLGSLREYMPKYGQGIGQGLLFAQQICLGMDYLHSKRYIHRDLAARNVLLENEGVVKIGDFGLTKYIPEGDIYYRVREDGDSPVFWYAIECLKESKFSFASDVWSFAVTLYEILTNCSHRLSPPSKFSDMMGTVHGQMTVVKLISLLEKNERLPCPPGCPREVYSLMEQCWDFNPDQRPSFLSLAESLEEIRGPYKQPSSLQLAQLNHR

Target ORF information:

RefSeq Version	XM_009299743.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299743.2

ORF Insert Sequence:

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tyk2 ( XM_009299743.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298018.1 Danio rerio tyrosine kinase 2 (tyk2), transcript variant X2, mRNA.

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CloneID	ODa08722
Clone ID Related Accession (Same CDS sequence)	XM_003198153.4 , XM_009299743.2 , XM_009299743.3 , XM_003198153.5
Accession Version	XM_009299743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	non-receptor tyrosine-protein kinase TYK2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299743.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298018.1
CDS	108..3605
Translation	MMYRRGRSRRSKKAGTSKGQSEGPGVHVFLFWTKDGERCLSYKKGEVTAEDLTTEAAKAVGITPVCHALFALYDPASLCWYSPNHNFNAENQSGLLLHFRLRFYFHKWHGLGENAPEVGRYALRTSAGTQQATSHLLELSALEYLFAQAKYDFVNDLVPLNAVSGEEEIGRYQNECLGMAVLHLSYTALQTGSSLQDVAKQTSFLRCIPLSFSRHIAKQNILTRFRIRRVFAHFVNTFQQHTVGVGKLGSQEVMYKYLSTLERLAPHFGVETFPVFHLDLRADGGDSGSYLITSRTQNPPEETINEPTHEIRVSGSDGIWWRKFSAQRSQSDDYSHSQNKETAALNEEEDWNIFCDFPEISLIAIQGINVCISRQDNMSMDISLDSSIQARSLVSLLDGYFRLTTDAHHYLCREVAPPRVVLSESNDLHGPLMDEFVLQKLKREVEEGAFLVRWSALDYHRIILASLSRSKKEGENPVYKQFRIRQNGSLFHLEGWDQDFSSIKELTSSLKTFVLKSGEDSFTLKKCCLPKPGELSNLLIIRKGAKSSIKSASETPDLSKLSFNPIKDKEITKEQHLGRGTRTNIYSGWLKIRGSLEDEDDESNNNHCKTKGIRVVLKILEQSQKDKALAFFETASLMSQVSHCHLIFVHGLSVKGTENIMVEEFVEFGPLDVFLHRERAHVSPQWKFTVAKQLASALCYLETKRLVHGNVCGKNILVVRKGLEEGTSPFVKLSDPGISLSALSQGERVERIPWIAPECVADGTRIGSAADKWSFGATLLEICNNGDLHVSNSTLSEKQRFYETQSRLAVPSSQELASFISRCLTYDAAARPSFRTILRELTEIQIKNPDISSECESLPDKDPSVFYKRYLRKIRDLGEGHFGKVMLYVYDPHNDGTGEYVAVKALKQEGDGSSLHVSWMKEIEILKSLYHNNIVKYKGCCTEMGGQVVQLIMEYVPLGSLREYMPKYGQGIGQGLLFAQQICLGMDYLHSKRYIHRDLAARNVLLENEGVVKIGDFGLTKYIPEGDIYYRVREDGDSPVFWYAIECLKESKFSFASDVWSFAVTLYEILTNCSHRLSPPSKFSDMMGTVHGQMTVVKLISLLEKNERLPCPPGCPREVYSLMEQCWDFNPDQRPSFLSLAESLEEIRGPYKQPSSLQLAQLNHR

Target ORF information:

RefSeq Version	XM_009299743.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299743.3

ORF Insert Sequence:

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tyk2 ( XM_003198153.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198201.2 Danio rerio tyrosine kinase 2 (tyk2), transcript variant X1, mRNA.

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CloneID	ODa08722
Clone ID Related Accession (Same CDS sequence)	XM_003198153.4 , XM_009299743.2 , XM_009299743.3 , XM_003198153.5
Accession Version	XM_003198153.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	non-receptor tyrosine-protein kinase TYK2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003198153.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198201.2
CDS	196..3693
Translation	MMYRRGRSRRSKKAGTSKGQSEGPGVHVFLFWTKDGERCLSYKKGEVTAEDLTTEAAKAVGITPVCHALFALYDPASLCWYSPNHNFNAENQSGLLLHFRLRFYFHKWHGLGENAPEVGRYALRTSAGTQQATSHLLELSALEYLFAQAKYDFVNDLVPLNAVSGEEEIGRYQNECLGMAVLHLSYTALQTGSSLQDVAKQTSFLRCIPLSFSRHIAKQNILTRFRIRRVFAHFVNTFQQHTVGVGKLGSQEVMYKYLSTLERLAPHFGVETFPVFHLDLRADGGDSGSYLITSRTQNPPEETINEPTHEIRVSGSDGIWWRKFSAQRSQSDDYSHSQNKETAALNEEEDWNIFCDFPEISLIAIQGINVCISRQDNMSMDISLDSSIQARSLVSLLDGYFRLTTDAHHYLCREVAPPRVVLSESNDLHGPLMDEFVLQKLKREVEEGAFLVRWSALDYHRIILASLSRSKKEGENPVYKQFRIRQNGSLFHLEGWDQDFSSIKELTSSLKTFVLKSGEDSFTLKKCCLPKPGELSNLLIIRKGAKSSIKSASETPDLSKLSFNPIKDKEITKEQHLGRGTRTNIYSGWLKIRGSLEDEDDESNNNHCKTKGIRVVLKILEQSQKDKALAFFETASLMSQVSHCHLIFVHGLSVKGTENIMVEEFVEFGPLDVFLHRERAHVSPQWKFTVAKQLASALCYLETKRLVHGNVCGKNILVVRKGLEEGTSPFVKLSDPGISLSALSQGERVERIPWIAPECVADGTRIGSAADKWSFGATLLEICNNGDLHVSNSTLSEKQRFYETQSRLAVPSSQELASFISRCLTYDAAARPSFRTILRELTEIQIKNPDISSECESLPDKDPSVFYKRYLRKIRDLGEGHFGKVMLYVYDPHNDGTGEYVAVKALKQEGDGSSLHVSWMKEIEILKSLYHNNIVKYKGCCTEMGGQVVQLIMEYVPLGSLREYMPKYGQGIGQGLLFAQQICLGMDYLHSKRYIHRDLAARNVLLENEGVVKIGDFGLTKYIPEGDIYYRVREDGDSPVFWYAIECLKESKFSFASDVWSFAVTLYEILTNCSHRLSPPSKFSDMMGTVHGQMTVVKLISLLEKNERLPCPPGCPREVYSLMEQCWDFNPDQRPSFLSLAESLEEIRGPYKQPSSLQLAQLNHR

Target ORF information:

RefSeq Version	XM_003198153.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198153.5

ORF Insert Sequence:

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGAAGGAAG GAGAAAATCC AGTCTACAAG 
CAGTTCCGAA TTAGACAGAA CGGCTCGTTG TTTCATTTGG AAGGCTGGGA TCAAGATTTC 
TCCAGCATAA AAGAGCTCAC CAGCAGCTTA AAAACCTTTG TGCTGAAGTC TGGAGAGGAC 
AGCTTCACTT TGAAGAAGTG CTGTCTGCCC AAACCAGGAG AGCTGTCAAA CCTTCTTATA 
ATAAGGAAGG GTGCCAAAAG CAGCATTAAA AGTGCCTCGG AAACCCCTGA CCTGAGCAAA 
CTGAGCTTCA ATCCAATTAA AGACAAGGAG ATTACAAAGG AGCAGCACCT GGGACGCGGC 
ACCAGGACTA ACATTTACTC CGGCTGGCTG AAGATCCGCG GTTCACTAGA GGATGAAGAT 
GATGAGTCCA ATAACAATCA CTGCAAAACA AAAGGGATCC GAGTTGTTCT CAAAATTTTG 
GAACAGAGCC AGAAGGACAA AGCGCTAGCG TTCTTCGAGA CGGCTAGTTT AATGAGTCAG 
GTGTCTCACT GTCATCTGAT CTTTGTTCAC GGACTTTCAG TCAAGGGTAC TGAGAACATC 
ATGGTGGAGG AGTTTGTGGA GTTCGGCCCC TTAGATGTGT TTCTTCACAG AGAAAGAGCT 
CATGTTTCTC CTCAGTGGAA GTTTACTGTC GCTAAACAGC TTGCTAGCGC TCTTTGCTAT 
CTTGAGACCA AGCGTTTAGT GCATGGAAAT GTCTGTGGCA AGAACATCCT GGTGGTCCGG 
AAAGGTTTAG AGGAGGGCAC GTCTCCTTTT GTAAAGCTCA GTGACCCAGG CATTTCTCTG 
TCAGCTCTAT CTCAAGGAGA GCGCGTGGAG CGAATTCCAT GGATTGCTCC AGAGTGTGTT 
GCTGACGGGA CTCGTATAGG AAGCGCTGCA GACAAGTGGA GCTTTGGAGC CACTCTGCTG 
GAAATCTGCA ACAACGGTGA TCTTCATGTC AGCAACAGCA CATTATCTGA GAAACAGCGC 
TTCTACGAGA CGCAGAGTCG CCTGGCGGTA CCTTCGTCTC AAGAGCTGGC CAGCTTCATC 
AGCAGGTGTC TGACATATGA TGCTGCTGCA CGGCCCTCCT TCCGGACTAT TCTGAGGGAG 
CTGACAGAGA TTCAGATAAA AAATCCAGAC ATTTCATCGG AGTGCGAGTC TCTTCCAGAC 
AAAGACCCAA GCGTCTTCTA CAAGCGTTAC CTGAGGAAGA TTCGGGATCT GGGAGAGGGT 
CACTTTGGAA AGGTGATGCT GTACGTCTAT GACCCTCACA ATGACGGGAC GGGCGAGTAT 
GTGGCGGTGA AGGCCTTAAA GCAGGAGGGC GACGGCAGCA GTCTCCATGT TTCCTGGATG 
AAGGAGATCG AAATCCTCAA ATCGCTTTAC CACAACAACA TTGTCAAATA TAAAGGCTGC 
TGTACTGAAA TGGGTGGACA GGTGGTTCAG TTAATCATGG AGTACGTCCC GCTGGGAAGC 
CTCCGAGAAT ACATGCCTAA ATACGGTCAG GGCATTGGAC AGGGTCTCCT GTTCGCCCAG 
CAGATCTGCC TGGGAATGGA CTATCTGCAC TCCAAGCGCT ATATCCATCG TGATCTGGCT 
GCGCGCAATG TTTTGTTGGA GAATGAAGGG GTGGTGAAGA TCGGTGACTT TGGCCTGACC 
AAGTACATTC CCGAAGGGGA CATTTACTAT CGTGTTCGAG AGGACGGGGA CAGTCCTGTG 
TTCTGGTATG CCATAGAGTG CCTGAAGGAG AGCAAGTTTT CCTTTGCCTC TGACGTTTGG 
TCTTTTGCTG TGACCCTTTA TGAGATTTTG ACTAACTGTA GCCATCGACT AAGCCCTCCT 
TCGAAATTCT CAGACATGAT GGGAACGGTC CACGGTCAGA TGACTGTTGT TAAACTGATT 
TCCCTCCTGG AGAAAAATGA GAGATTACCA TGCCCTCCAG GCTGTCCTCG TGAGGTGTAT 
TCACTGATGG AGCAGTGCTG GGACTTCAAC CCTGATCAAC GTCCGTCGTT CTTATCGCTG 
GCTGAGTCTC TGGAGGAAAT TCGAGGACCA TACAAACAGC CGTCGTCGTT GCAGCTCGCT 
CAGCTCAACC ATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tyk2 ( XM_005164236.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164293.1 Danio rerio tyrosine kinase 2 (tyk2), transcript variant X3, mRNA.

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CloneID	ODa08723
Clone ID Related Accession (Same CDS sequence)	XM_005164236.4 , XM_005164236.3
Accession Version	XM_005164236.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	non-receptor tyrosine-protein kinase TYK2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164236.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGGAAGGAG AAAATCCAGT CTACAAGCAG 
TTCCGAATTA GACAGAACGG CTCGTTGTTT CATTTGGAAG GCTGGGATCA AGATTTCTCC 
AGCATAAAAG AGCTCACCAG CAGCTTAAAA ACCTTTGTGC TGAAGTCTGG AGAGGACAGC 
TTCACTTTGA AGAAGTGCTG TCTGCCCAAA CCAGGAGAGC TGTCAAACCT TCTTATAATA 
AGGAAGGGTG CCAAAAGCAG CATTAAAAGT GCCTCGGAAA CCCCTGACCT GAGCAAACTG 
AGCTTCAATC CAATTAAAGA CAAGGAGATT ACAAAGGAGC AGCACCTGGG ACGCGGCACC 
AGGACTAACA TTTACTCCGG CTGGCTGAAG ATCCGCGGTT CACTAGAGGA TGAAGATGAT 
GAGTCCAATA ACAATCACTG CAAAACAAAA GGGATCCGAG TTGTTCTCAA AATTTTGGAA 
CAGAGCCAGA AGGACAAAGC GCTAGCGTTC TTCGAGACGG CTAGTTTAAT GAGTCAGGTG 
TCTCACTGTC ATCTGATCTT TGTTCACGGA CTTTCAGTCA AGGGTACTGA GAACATCATG 
GTGGAGGAGT TTGTGGAGTT CGGCCCCTTA GATGTGTTTC TTCACAGAGA AAGAGCTCAT 
GTTTCTCCTC AGTGGAAGTT TACTGTCGCT AAACAGCTTG CTAGCGCTCT TTGCTATCTT 
GAGACCAAGC GTTTAGTGCA TGGAAATGTC TGTGGCAAGA ACATCCTGGT GGTCCGGAAA 
GGTTTAGAGG AGGGCACGTC TCCTTTTGTA AAGCTCAGTG ACCCAGGCAT TTCTCTGTCA 
GCTCTATCTC AAGGAGAGCG CGTGGAGCGA ATTCCATGGA TTGCTCCAGA GTGTGTTGCT 
GACGGGACTC GTATAGGAAG CGCTGCAGAC AAGTGGAGCT TTGGAGCCAC TCTGCTGGAA 
ATCTGCAACA ACGGTGATCT TCATGTCAGC AACAGCACAT TATCTGAGAA ACAGCGCTTC 
TACGAGACGC AGAGTCGCCT GGCGGTACCT TCGTCTCAAG AGCTGGCCAG CTTCATCAGC 
AGGTGTCTGA CATATGATGC TGCTGCACGG CCCTCCTTCC GGACTATTCT GAGGGAGCTG 
ACAGAGATTC AGATAAAAAA TCCAGACATT TCATCGGAGT GCGAGTCTCT TCCAGACAAA 
GACCCAAGCG TCTTCTACAA GCGTTACCTG AGGAAGATTC GGGATCTGGG AGAGGGTCAC 
TTTGGAAAGG TGATGCTGTA CGTCTATGAC CCTCACAATG ACGGGACGGG CGAGTATGTG 
GCGGTGAAGG CCTTAAAGCA GGAGGGCGAC GGCAGCAGTC TCCATGTTTC CTGGATGAAG 
GAGATCGAAA TCCTCAAATC GCTTTACCAC AACAACATTG TCAAATATAA AGGCTGCTGT 
ACTGAAATGG GTGGACAGGT GGTTCAGTTA ATCATGGAGT ACGTCCCGCT GGGAAGCCTC 
CGAGAATACA TGCCTAAATA CGGTCAGGGC ATTGGACAGG GTCTCCTGTT CGCCCAGCAG 
ATCTGCCTGG GAATGGACTA TCTGCACTCC AAGCGCTATA TCCATCGTGA TCTGGCTGCG 
CGCAATGTTT TGTTGGAGAA TGAAGGGGTG GTGAAGATCG GTGACTTTGG CCTGACCAAG 
TACATTCCCG AAGGGGACAT TTACTATCGT GTTCGAGAGG ACGGGGACAG TCCTGTGTTC 
TGGTATGCCA TAGAGTGCCT GAAGGAGAGC AAGTTTTCCT TTGCCTCTGA CGTTTGGTCT 
TTTGCTGTGA CCCTTTATGA GATTTTGACT AACTGTAGCC ATCGACTAAG CCCTCCTTCG 
AAATTCTCAG ACATGATGGG AACGGTCCAC GGTCAGATGA CTGTTGTTAA ACTGATTTCC 
CTCCTGGAGA AAAATGAGAG ATTACCATGC CCTCCAGGCT GTCCTCGTGA GGTGTATTCA 
CTGATGGAGC AGTGCTGGGA CTTCAACCCT GATCAACGTC CGTCGTTCTT ATCGCTGGCT 
GAGTCTCTGG AGGAAATTCG AGGACCATAC AAACAGCCGT CGTCGTTGCA GCTCGCTCAG 
CTCAACCATC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164293.1
CDS	196..3690
Translation	MMYRRGRSRRSKKAGTSKGQSEGPGVHVFLFWTKDGERCLSYKKGEVTAEDLTTEAAKAVGITPVCHALFALYDPASLCWYSPNHNFNAENQSGLLLHFRLRFYFHKWHGLGENAPEVGRYALRTSAGTQQATSHLLELSALEYLFAQAKYDFVNDLVPLNAVSGEEEIGRYQNECLGMAVLHLSYTALQTGSSLQDVAKQTSFLRCIPLSFSRHIAKQNILTRFRIRRVFAHFVNTFQQHTVGVGKLGSQEVMYKYLSTLERLAPHFGVETFPVFHLDLRADGGDSGSYLITSRTQNPPEETINEPTHEIRVSGSDGIWWRKFSAQRSQSDDYSHSQNKETAALNEEEDWNIFCDFPEISLIAIQGINVCISRQDNMSMDISLDSSIQARSLVSLLDGYFRLTTDAHHYLCREVAPPRVVLSESNDLHGPLMDEFVLQKLKREVEEGAFLVRWSALDYHRIILASLSRSKEGENPVYKQFRIRQNGSLFHLEGWDQDFSSIKELTSSLKTFVLKSGEDSFTLKKCCLPKPGELSNLLIIRKGAKSSIKSASETPDLSKLSFNPIKDKEITKEQHLGRGTRTNIYSGWLKIRGSLEDEDDESNNNHCKTKGIRVVLKILEQSQKDKALAFFETASLMSQVSHCHLIFVHGLSVKGTENIMVEEFVEFGPLDVFLHRERAHVSPQWKFTVAKQLASALCYLETKRLVHGNVCGKNILVVRKGLEEGTSPFVKLSDPGISLSALSQGERVERIPWIAPECVADGTRIGSAADKWSFGATLLEICNNGDLHVSNSTLSEKQRFYETQSRLAVPSSQELASFISRCLTYDAAARPSFRTILRELTEIQIKNPDISSECESLPDKDPSVFYKRYLRKIRDLGEGHFGKVMLYVYDPHNDGTGEYVAVKALKQEGDGSSLHVSWMKEIEILKSLYHNNIVKYKGCCTEMGGQVVQLIMEYVPLGSLREYMPKYGQGIGQGLLFAQQICLGMDYLHSKRYIHRDLAARNVLLENEGVVKIGDFGLTKYIPEGDIYYRVREDGDSPVFWYAIECLKESKFSFASDVWSFAVTLYEILTNCSHRLSPPSKFSDMMGTVHGQMTVVKLISLLEKNERLPCPPGCPREVYSLMEQCWDFNPDQRPSFLSLAESLEEIRGPYKQPSSLQLAQLNHR

Target ORF information:

RefSeq Version	XM_005164236.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164236.4

ORF Insert Sequence:

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGGAAGGAG AAAATCCAGT CTACAAGCAG 
TTCCGAATTA GACAGAACGG CTCGTTGTTT CATTTGGAAG GCTGGGATCA AGATTTCTCC 
AGCATAAAAG AGCTCACCAG CAGCTTAAAA ACCTTTGTGC TGAAGTCTGG AGAGGACAGC 
TTCACTTTGA AGAAGTGCTG TCTGCCCAAA CCAGGAGAGC TGTCAAACCT TCTTATAATA 
AGGAAGGGTG CCAAAAGCAG CATTAAAAGT GCCTCGGAAA CCCCTGACCT GAGCAAACTG 
AGCTTCAATC CAATTAAAGA CAAGGAGATT ACAAAGGAGC AGCACCTGGG ACGCGGCACC 
AGGACTAACA TTTACTCCGG CTGGCTGAAG ATCCGCGGTT CACTAGAGGA TGAAGATGAT 
GAGTCCAATA ACAATCACTG CAAAACAAAA GGGATCCGAG TTGTTCTCAA AATTTTGGAA 
CAGAGCCAGA AGGACAAAGC GCTAGCGTTC TTCGAGACGG CTAGTTTAAT GAGTCAGGTG 
TCTCACTGTC ATCTGATCTT TGTTCACGGA CTTTCAGTCA AGGGTACTGA GAACATCATG 
GTGGAGGAGT TTGTGGAGTT CGGCCCCTTA GATGTGTTTC TTCACAGAGA AAGAGCTCAT 
GTTTCTCCTC AGTGGAAGTT TACTGTCGCT AAACAGCTTG CTAGCGCTCT TTGCTATCTT 
GAGACCAAGC GTTTAGTGCA TGGAAATGTC TGTGGCAAGA ACATCCTGGT GGTCCGGAAA 
GGTTTAGAGG AGGGCACGTC TCCTTTTGTA AAGCTCAGTG ACCCAGGCAT TTCTCTGTCA 
GCTCTATCTC AAGGAGAGCG CGTGGAGCGA ATTCCATGGA TTGCTCCAGA GTGTGTTGCT 
GACGGGACTC GTATAGGAAG CGCTGCAGAC AAGTGGAGCT TTGGAGCCAC TCTGCTGGAA 
ATCTGCAACA ACGGTGATCT TCATGTCAGC AACAGCACAT TATCTGAGAA ACAGCGCTTC 
TACGAGACGC AGAGTCGCCT GGCGGTACCT TCGTCTCAAG AGCTGGCCAG CTTCATCAGC 
AGGTGTCTGA CATATGATGC TGCTGCACGG CCCTCCTTCC GGACTATTCT GAGGGAGCTG 
ACAGAGATTC AGATAAAAAA TCCAGACATT TCATCGGAGT GCGAGTCTCT TCCAGACAAA 
GACCCAAGCG TCTTCTACAA GCGTTACCTG AGGAAGATTC GGGATCTGGG AGAGGGTCAC 
TTTGGAAAGG TGATGCTGTA CGTCTATGAC CCTCACAATG ACGGGACGGG CGAGTATGTG 
GCGGTGAAGG CCTTAAAGCA GGAGGGCGAC GGCAGCAGTC TCCATGTTTC CTGGATGAAG 
GAGATCGAAA TCCTCAAATC GCTTTACCAC AACAACATTG TCAAATATAA AGGCTGCTGT 
ACTGAAATGG GTGGACAGGT GGTTCAGTTA ATCATGGAGT ACGTCCCGCT GGGAAGCCTC 
CGAGAATACA TGCCTAAATA CGGTCAGGGC ATTGGACAGG GTCTCCTGTT CGCCCAGCAG 
ATCTGCCTGG GAATGGACTA TCTGCACTCC AAGCGCTATA TCCATCGTGA TCTGGCTGCG 
CGCAATGTTT TGTTGGAGAA TGAAGGGGTG GTGAAGATCG GTGACTTTGG CCTGACCAAG 
TACATTCCCG AAGGGGACAT TTACTATCGT GTTCGAGAGG ACGGGGACAG TCCTGTGTTC 
TGGTATGCCA TAGAGTGCCT GAAGGAGAGC AAGTTTTCCT TTGCCTCTGA CGTTTGGTCT 
TTTGCTGTGA CCCTTTATGA GATTTTGACT AACTGTAGCC ATCGACTAAG CCCTCCTTCG 
AAATTCTCAG ACATGATGGG AACGGTCCAC GGTCAGATGA CTGTTGTTAA ACTGATTTCC 
CTCCTGGAGA AAAATGAGAG ATTACCATGC CCTCCAGGCT GTCCTCGTGA GGTGTATTCA 
CTGATGGAGC AGTGCTGGGA CTTCAACCCT GATCAACGTC CGTCGTTCTT ATCGCTGGCT 
GAGTCTCTGG AGGAAATTCG AGGACCATAC AAACAGCCGT CGTCGTTGCA GCTCGCTCAG 
CTCAACCATC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tyk2 ( XM_005164236.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164293.1 Danio rerio tyrosine kinase 2 (tyk2), transcript variant X3, mRNA.

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CloneID	ODa08723
Clone ID Related Accession (Same CDS sequence)	XM_005164236.4 , XM_005164236.3
Accession Version	XM_005164236.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	non-receptor tyrosine-protein kinase TYK2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGGAAGGAG AAAATCCAGT CTACAAGCAG 
TTCCGAATTA GACAGAACGG CTCGTTGTTT CATTTGGAAG GCTGGGATCA AGATTTCTCC 
AGCATAAAAG AGCTCACCAG CAGCTTAAAA ACCTTTGTGC TGAAGTCTGG AGAGGACAGC 
TTCACTTTGA AGAAGTGCTG TCTGCCCAAA CCAGGAGAGC TGTCAAACCT TCTTATAATA 
AGGAAGGGTG CCAAAAGCAG CATTAAAAGT GCCTCGGAAA CCCCTGACCT GAGCAAACTG 
AGCTTCAATC CAATTAAAGA CAAGGAGATT ACAAAGGAGC AGCACCTGGG ACGCGGCACC 
AGGACTAACA TTTACTCCGG CTGGCTGAAG ATCCGCGGTT CACTAGAGGA TGAAGATGAT 
GAGTCCAATA ACAATCACTG CAAAACAAAA GGGATCCGAG TTGTTCTCAA AATTTTGGAA 
CAGAGCCAGA AGGACAAAGC GCTAGCGTTC TTCGAGACGG CTAGTTTAAT GAGTCAGGTG 
TCTCACTGTC ATCTGATCTT TGTTCACGGA CTTTCAGTCA AGGGTACTGA GAACATCATG 
GTGGAGGAGT TTGTGGAGTT CGGCCCCTTA GATGTGTTTC TTCACAGAGA AAGAGCTCAT 
GTTTCTCCTC AGTGGAAGTT TACTGTCGCT AAACAGCTTG CTAGCGCTCT TTGCTATCTT 
GAGACCAAGC GTTTAGTGCA TGGAAATGTC TGTGGCAAGA ACATCCTGGT GGTCCGGAAA 
GGTTTAGAGG AGGGCACGTC TCCTTTTGTA AAGCTCAGTG ACCCAGGCAT TTCTCTGTCA 
GCTCTATCTC AAGGAGAGCG CGTGGAGCGA ATTCCATGGA TTGCTCCAGA GTGTGTTGCT 
GACGGGACTC GTATAGGAAG CGCTGCAGAC AAGTGGAGCT TTGGAGCCAC TCTGCTGGAA 
ATCTGCAACA ACGGTGATCT TCATGTCAGC AACAGCACAT TATCTGAGAA ACAGCGCTTC 
TACGAGACGC AGAGTCGCCT GGCGGTACCT TCGTCTCAAG AGCTGGCCAG CTTCATCAGC 
AGGTGTCTGA CATATGATGC TGCTGCACGG CCCTCCTTCC GGACTATTCT GAGGGAGCTG 
ACAGAGATTC AGATAAAAAA TCCAGACATT TCATCGGAGT GCGAGTCTCT TCCAGACAAA 
GACCCAAGCG TCTTCTACAA GCGTTACCTG AGGAAGATTC GGGATCTGGG AGAGGGTCAC 
TTTGGAAAGG TGATGCTGTA CGTCTATGAC CCTCACAATG ACGGGACGGG CGAGTATGTG 
GCGGTGAAGG CCTTAAAGCA GGAGGGCGAC GGCAGCAGTC TCCATGTTTC CTGGATGAAG 
GAGATCGAAA TCCTCAAATC GCTTTACCAC AACAACATTG TCAAATATAA AGGCTGCTGT 
ACTGAAATGG GTGGACAGGT GGTTCAGTTA ATCATGGAGT ACGTCCCGCT GGGAAGCCTC 
CGAGAATACA TGCCTAAATA CGGTCAGGGC ATTGGACAGG GTCTCCTGTT CGCCCAGCAG 
ATCTGCCTGG GAATGGACTA TCTGCACTCC AAGCGCTATA TCCATCGTGA TCTGGCTGCG 
CGCAATGTTT TGTTGGAGAA TGAAGGGGTG GTGAAGATCG GTGACTTTGG CCTGACCAAG 
TACATTCCCG AAGGGGACAT TTACTATCGT GTTCGAGAGG ACGGGGACAG TCCTGTGTTC 
TGGTATGCCA TAGAGTGCCT GAAGGAGAGC AAGTTTTCCT TTGCCTCTGA CGTTTGGTCT 
TTTGCTGTGA CCCTTTATGA GATTTTGACT AACTGTAGCC ATCGACTAAG CCCTCCTTCG 
AAATTCTCAG ACATGATGGG AACGGTCCAC GGTCAGATGA CTGTTGTTAA ACTGATTTCC 
CTCCTGGAGA AAAATGAGAG ATTACCATGC CCTCCAGGCT GTCCTCGTGA GGTGTATTCA 
CTGATGGAGC AGTGCTGGGA CTTCAACCCT GATCAACGTC CGTCGTTCTT ATCGCTGGCT 
GAGTCTCTGG AGGAAATTCG AGGACCATAC AAACAGCCGT CGTCGTTGCA GCTCGCTCAG 
CTCAACCATC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164293.1
CDS	182..3676
Translation	MMYRRGRSRRSKKAGTSKGQSEGPGVHVFLFWTKDGERCLSYKKGEVTAEDLTTEAAKAVGITPVCHALFALYDPASLCWYSPNHNFNAENQSGLLLHFRLRFYFHKWHGLGENAPEVGRYALRTSAGTQQATSHLLELSALEYLFAQAKYDFVNDLVPLNAVSGEEEIGRYQNECLGMAVLHLSYTALQTGSSLQDVAKQTSFLRCIPLSFSRHIAKQNILTRFRIRRVFAHFVNTFQQHTVGVGKLGSQEVMYKYLSTLERLAPHFGVETFPVFHLDLRADGGDSGSYLITSRTQNPPEETINEPTHEIRVSGSDGIWWRKFSAQRSQSDDYSHSQNKETAALNEEEDWNIFCDFPEISLIAIQGINVCISRQDNMSMDISLDSSIQARSLVSLLDGYFRLTTDAHHYLCREVAPPRVVLSESNDLHGPLMDEFVLQKLKREVEEGAFLVRWSALDYHRIILASLSRSKEGENPVYKQFRIRQNGSLFHLEGWDQDFSSIKELTSSLKTFVLKSGEDSFTLKKCCLPKPGELSNLLIIRKGAKSSIKSASETPDLSKLSFNPIKDKEITKEQHLGRGTRTNIYSGWLKIRGSLEDEDDESNNNHCKTKGIRVVLKILEQSQKDKALAFFETASLMSQVSHCHLIFVHGLSVKGTENIMVEEFVEFGPLDVFLHRERAHVSPQWKFTVAKQLASALCYLETKRLVHGNVCGKNILVVRKGLEEGTSPFVKLSDPGISLSALSQGERVERIPWIAPECVADGTRIGSAADKWSFGATLLEICNNGDLHVSNSTLSEKQRFYETQSRLAVPSSQELASFISRCLTYDAAARPSFRTILRELTEIQIKNPDISSECESLPDKDPSVFYKRYLRKIRDLGEGHFGKVMLYVYDPHNDGTGEYVAVKALKQEGDGSSLHVSWMKEIEILKSLYHNNIVKYKGCCTEMGGQVVQLIMEYVPLGSLREYMPKYGQGIGQGLLFAQQICLGMDYLHSKRYIHRDLAARNVLLENEGVVKIGDFGLTKYIPEGDIYYRVREDGDSPVFWYAIECLKESKFSFASDVWSFAVTLYEILTNCSHRLSPPSKFSDMMGTVHGQMTVVKLISLLEKNERLPCPPGCPREVYSLMEQCWDFNPDQRPSFLSLAESLEEIRGPYKQPSSLQLAQLNHR

Target ORF information:

RefSeq Version	XM_005164236.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tyrosine kinase 2 (tyk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164236.3

ORF Insert Sequence:

ATGATGTATA GACGAGGACG TTCTAGGAGA TCAAAGAAAG CAGGGACGTC CAAAGGACAG 
AGTGAAGGTC CTGGTGTCCA TGTGTTTCTG TTCTGGACCA AAGATGGAGA ACGATGCCTG 
TCTTACAAGA AGGGTGAGGT GACTGCTGAA GATCTCACCA CTGAAGCAGC AAAGGCTGTG 
GGGATCACTC CAGTCTGTCA TGCCTTGTTT GCACTGTATG ACCCTGCTTC ATTGTGCTGG 
TACAGTCCAA ATCACAACTT TAATGCAGAG AACCAGTCGG GCCTGTTACT GCATTTTCGT 
TTGAGATTTT ACTTTCATAA GTGGCATGGA TTAGGTGAAA ATGCACCAGA AGTGGGCCGC 
TATGCATTAC GGACCTCAGC TGGGACACAG CAGGCGACAT CTCATTTGCT GGAGCTGAGT 
GCCCTGGAAT ACCTGTTTGC TCAGGCAAAA TATGATTTTG TGAATGACTT GGTGCCGTTG 
AATGCAGTGA GTGGAGAGGA AGAGATCGGT CGCTATCAGA ATGAATGTCT GGGTATGGCT 
GTGCTTCACC TTTCCTACAC GGCTCTGCAG ACCGGCTCTT CTCTACAGGA TGTGGCCAAG 
CAGACCAGCT TTCTCCGCTG CATTCCTCTG TCTTTTTCGC GACACATTGC CAAACAGAAC 
ATCCTGACGA GATTCCGGAT TCGGCGAGTA TTTGCACACT TTGTGAACAC CTTCCAGCAA 
CACACGGTGG GCGTGGGCAA GCTTGGATCA CAAGAAGTCA TGTACAAATA CTTGTCCACA 
CTGGAGCGCC TGGCACCCCA TTTCGGCGTC GAGACTTTCC CTGTTTTCCA TCTGGACCTC 
AGAGCTGATG GAGGTGACAG TGGCTCATAC CTCATCACCT CCCGTACACA AAACCCACCT 
GAGGAGACCA TAAATGAACC TACTCATGAG ATCAGGGTGT CTGGATCAGA TGGCATATGG 
TGGAGGAAGT TTTCGGCACA GCGGAGCCAG AGCGATGACT ACAGTCATAG TCAGAACAAA 
GAAACAGCGG CACTTAATGA GGAGGAGGAC TGGAACATCT TCTGCGATTT CCCAGAGATC 
TCTCTGATTG CCATTCAGGG CATCAATGTG TGCATCAGCC GACAGGACAA CATGTCAATG 
GACATAAGTT TAGACTCCAG TATTCAGGCT CGCTCTCTGG TCTCACTGCT CGATGGATAT 
TTCCGACTTA CGACCGATGC TCATCATTAC CTCTGTCGTG AAGTGGCTCC GCCGAGAGTG 
GTTCTCAGTG AGTCCAACGA CCTGCATGGT CCCTTAATGG ATGAGTTTGT GCTCCAGAAA 
CTGAAGAGAG AAGTGGAGGA GGGTGCATTT CTTGTGCGCT GGAGTGCTTT GGATTACCAT 
CGCATAATTC TGGCTTCTCT GAGTCGGTCA AAGGAAGGAG AAAATCCAGT CTACAAGCAG 
TTCCGAATTA GACAGAACGG CTCGTTGTTT CATTTGGAAG GCTGGGATCA AGATTTCTCC 
AGCATAAAAG AGCTCACCAG CAGCTTAAAA ACCTTTGTGC TGAAGTCTGG AGAGGACAGC 
TTCACTTTGA AGAAGTGCTG TCTGCCCAAA CCAGGAGAGC TGTCAAACCT TCTTATAATA 
AGGAAGGGTG CCAAAAGCAG CATTAAAAGT GCCTCGGAAA CCCCTGACCT GAGCAAACTG 
AGCTTCAATC CAATTAAAGA CAAGGAGATT ACAAAGGAGC AGCACCTGGG ACGCGGCACC 
AGGACTAACA TTTACTCCGG CTGGCTGAAG ATCCGCGGTT CACTAGAGGA TGAAGATGAT 
GAGTCCAATA ACAATCACTG CAAAACAAAA GGGATCCGAG TTGTTCTCAA AATTTTGGAA 
CAGAGCCAGA AGGACAAAGC GCTAGCGTTC TTCGAGACGG CTAGTTTAAT GAGTCAGGTG 
TCTCACTGTC ATCTGATCTT TGTTCACGGA CTTTCAGTCA AGGGTACTGA GAACATCATG 
GTGGAGGAGT TTGTGGAGTT CGGCCCCTTA GATGTGTTTC TTCACAGAGA AAGAGCTCAT 
GTTTCTCCTC AGTGGAAGTT TACTGTCGCT AAACAGCTTG CTAGCGCTCT TTGCTATCTT 
GAGACCAAGC GTTTAGTGCA TGGAAATGTC TGTGGCAAGA ACATCCTGGT GGTCCGGAAA 
GGTTTAGAGG AGGGCACGTC TCCTTTTGTA AAGCTCAGTG ACCCAGGCAT TTCTCTGTCA 
GCTCTATCTC AAGGAGAGCG CGTGGAGCGA ATTCCATGGA TTGCTCCAGA GTGTGTTGCT 
GACGGGACTC GTATAGGAAG CGCTGCAGAC AAGTGGAGCT TTGGAGCCAC TCTGCTGGAA 
ATCTGCAACA ACGGTGATCT TCATGTCAGC AACAGCACAT TATCTGAGAA ACAGCGCTTC 
TACGAGACGC AGAGTCGCCT GGCGGTACCT TCGTCTCAAG AGCTGGCCAG CTTCATCAGC 
AGGTGTCTGA CATATGATGC TGCTGCACGG CCCTCCTTCC GGACTATTCT GAGGGAGCTG 
ACAGAGATTC AGATAAAAAA TCCAGACATT TCATCGGAGT GCGAGTCTCT TCCAGACAAA 
GACCCAAGCG TCTTCTACAA GCGTTACCTG AGGAAGATTC GGGATCTGGG AGAGGGTCAC 
TTTGGAAAGG TGATGCTGTA CGTCTATGAC CCTCACAATG ACGGGACGGG CGAGTATGTG 
GCGGTGAAGG CCTTAAAGCA GGAGGGCGAC GGCAGCAGTC TCCATGTTTC CTGGATGAAG 
GAGATCGAAA TCCTCAAATC GCTTTACCAC AACAACATTG TCAAATATAA AGGCTGCTGT 
ACTGAAATGG GTGGACAGGT GGTTCAGTTA ATCATGGAGT ACGTCCCGCT GGGAAGCCTC 
CGAGAATACA TGCCTAAATA CGGTCAGGGC ATTGGACAGG GTCTCCTGTT CGCCCAGCAG 
ATCTGCCTGG GAATGGACTA TCTGCACTCC AAGCGCTATA TCCATCGTGA TCTGGCTGCG 
CGCAATGTTT TGTTGGAGAA TGAAGGGGTG GTGAAGATCG GTGACTTTGG CCTGACCAAG 
TACATTCCCG AAGGGGACAT TTACTATCGT GTTCGAGAGG ACGGGGACAG TCCTGTGTTC 
TGGTATGCCA TAGAGTGCCT GAAGGAGAGC AAGTTTTCCT TTGCCTCTGA CGTTTGGTCT 
TTTGCTGTGA CCCTTTATGA GATTTTGACT AACTGTAGCC ATCGACTAAG CCCTCCTTCG 
AAATTCTCAG ACATGATGGG AACGGTCCAC GGTCAGATGA CTGTTGTTAA ACTGATTTCC 
CTCCTGGAGA AAAATGAGAG ATTACCATGC CCTCCAGGCT GTCCTCGTGA GGTGTATTCA 
CTGATGGAGC AGTGCTGGGA CTTCAACCCT GATCAACGTC CGTCGTTCTT ATCGCTGGCT 
GAGTCTCTGG AGGAAATTCG AGGACCATAC AAACAGCCGT CGTCGTTGCA GCTCGCTCAG 
CTCAACCATC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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tyk2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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