LOC100535772 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100535772
Entrez Gene ID	100535772
Full Name	ankyrin repeat and fibronectin type-III domain-containing protein 1-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353975.2	XP_017209464.1	ankyrin repeat and fibronectin type-III domain-containing protein 1-like isoform X2
XM_017353975.1	XP_017209464.1	ankyrin repeat and fibronectin type-III domain-containing protein 1-like
XM_021470404.1	XP_021326079.1	ankyrin repeat and fibronectin type-III domain-containing protein 1-like isoform X1
XM_021470405.1	XP_021326080.1	ankyrin repeat and fibronectin type-III domain-containing protein 1-like isoform X3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ankfn1	XP_004918051.1
Danio rerio (zebrafish)	LOC100535772	XP_003201405.1
Gallus gallus (chicken)	LOC416752	XP_004945511.1

Gene Ontology

The following LOC100535772 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100535772 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141282	XM_021470404.1 Latest version!	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa28886	XM_021470405.1 Latest version!	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa57726	XM_017353975.2 Latest version!	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
ODa57726	XM_017353975.1	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100535772 ( XM_021470404.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326079.1 Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa141282
Clone ID Related Accession (Same CDS sequence)	XM_021470404.1
Accession Version	XM_021470404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGTT TTGGGAAACT TACGGATTAC TTTAACCGTC GGCGTTCCAG GGATGAGCTC 
ATGGCTCGGA GAGATGCGCG ACGCAGCGGC AGTTACTGCT GTACGGTGGC AGAAGCCAGG 
TCTCTAGAGA GACGCCTGCA GAGGCCTCTG TTGGCCAAAT CTAGAACATT ACCCAGCATC 
CCTCAGTCCC CTGCTGCAGT CCGTGTGCAG CAGACGGAGA GTGATTGTGA GCGCCAACAA 
CGGCCTCGTG TTCCTTCTAG CCCTGGGGGT CTTGAAGCCT GCACCATTCC ACCTGCAGGT 
GAGATGTGGA GGCTGGAGGA TGAGAGCGAG GCAGACATGG CTCCAGGTGC CGTTGACCTG 
CGTCCTCGCT CTCAGTCACC GTTCTCACAT TTCAGACGGC GAGCGGCGTA TCTGAGGAAG 
AGCATCTCAG CGGACGATCA TCTAGTGGAT TCAGAGTTCC AGAGCATCCA GACAGAGGAC 
AGGAGCTCAG CTGATCCCAA AGCTAAACTC AAGAGGAAGT TTAGTCTGGG CTCCATCGAC 
AGGAAAGGAA CGCGACAGAG AAGGACTGAA TCTGGGATTG TGAAACTGAC CCAGCGACTC 
TCTCTGAAGG ACAAACACTG CAGTAAAGAG GACAGGAGTC TGCAGTCCAG ACCTCCGGCC 
TACAGGAGGC GCTCTCTCAG CGTGGACTGG GCCGAATCCA AAATGACTCA ACAGATTCCA 
GACCCTCATC CTCTGATTGC ACGGAAATAC AGCACATCAT GTTCGTCTCC ACCGAGCGCA 
GCGCGGCGTC TCTACAGGAA CCTGTCGGGG AAATTCAGAA CAGCAAACCT GGTTTTGGAT 
GATACGACAC TCCCGGGACA GTCGGACAAA GAGCAGTCAC ACAAAACCAC CATGTTCCAG 
GGTAACGAGG CTCTGTTTGA AGCGGTGGAG CATCAGGAGC TGGACGTGGT GGAGTCTCTG 
CTGAACACCT TCAGTCTGGA GGAGCTGGAC TTGAACACAC CCAACAGTGA AGGCCTGCTG 
CCGCTCGACA TCGCCATCAT GACCAACAAC GTGCCCATGG CCAAACTGCT GCTGAGAGCC 
GGGGCCAAAG AGAGTCCCCA CTTTTTAAGT CTTGAAGGTC GAGCTGCTCA CCTGGCTTCT 
CTGGTGCAAG AGGCAGAGCA TCGTATAGCA GAGCTGGTTG CCCAGGTGAC AGGGGAGGAG 
CCTGGAGAGA GGGAGGAGTC AAACAGAGAG AGACAGCTGA AATCATGGGA ATGGAAGCTC 
CGCCTCTATA AGCGCATGCA GACGGGATAC ACACATGCCA GTCCTCCCGA ACCTCCCAGT 
GACGTACATC TGTGTGTCAG CAGTGGCACC AGTCTCAGAG TTTACTTCCA AGAGCCTCTA 
TGTCACAATT CGGCTGTTAT AACCAAATAC CGCGTGAGCT GGAGCAGTGT TTCTTCATTT 
AGCCCTTTGC TGGGTGAGAC GGTCATTGAA GACGTGACTC AACTCCAGTA TGACATCACA 
GGACTCGCTG CGGGCACAGG CTGTTATGTC CAGGTCTCAG CATATAATAT GAAGGGATGG 
AGTTCACCTC AGATATCAGA ACCTGCTTGT GCTACACCCT CCAGTTGGAG GGAGGTGGAT 
GGTCTTCCCC CCCGCCAGCA GGGTCTGAAA GAGGCGTTAG AGCAGCTCCT GGGGCAAATT 
AAAGGGGCGC ACCGTCACTG CGCATGCCAC GAACAAATCA AAGCTCCGTC GAACACCAGA 
AAACATTCTG TCTCTAAAAG TCTGCGGCAT CTCTTCCAGC CCACCAGCAA ATTTGTCAAA 
AACCTGAAAA GAGGTCTTTA TCTAGCCTGT ATATTTTATC AAGATGAAAA CGTTCTGGTG 
ACTCCAGAGG ATCAGCTGCC GGTTGTAGAG GTGGACGACT CCTTCAGCTG TCACGCACAG 
GATTTACTCT GGTTCACAAA GGTGTGTCAC CTGTGGGAGG AGTTGAGCTG GATGCAGCAG 
TGTATCAGTC CTGCTCACGC CTGCAGCTCC TGCACACTTC AGACACGCCT CAAACTGCTG 
CAGGCAGCGG CACAACTACA GGCTCTGCTG GGCACGGTGG ATCTGGGACA GGTGTATTTC 
GAGCCCATTA AAGACAAGCA CGGTAATGCA GTGCTGGTGC TGCTGAAGGA CATGAGTGCG 
TGTGCGCCGC TGGAGGGACT GCGCTGGATT CCTGTCAGTA AATTCCAGCT TCAGCGAAGG 
TCTTCCTCGT CTTCATCAGA AGAGCCCAGC GCACTGGAGA CGCTCCTCTC CACACTGCAC 
GAGAAGCTGT CGTATCACCG GCGCAGCAGG AAGTGTCTGC CTCCAGGTCT GTATCTGGGT 
TACTTGAAGC TCTACACCTC AGTGGACCAG ATCCGAGTCC TGGTGTCACA CAAACACCCA 
AACGTGCTCT GCCACGTCAA GATACGAGAC AACGGCCACG TCTCCAGGGA GGAATGGCAG 
TGGCTTCAGT CACTGACTTC ACTGGAGGAC AGCGCGAAGG TGTGTGAGGA TGCTCAGAGT 
GCTCCGCATC GCCTCCTGCT GGACTTACGA ACCGCCGCCA AAGAGTTACT CGCATACGTC 
AACATTCCCA ACCAGCAGGC GCAGGACTTC CGTGTGTATG TGAAGGAGGT GGTGGAGCTG 
GGTGAAGGAG TCTCATTCCT GCTACTGCTG CCGCCCTGTG AGGAGGTGTG TTCACCACCG 
GGACAAAACC ACCTGTGCTC ACCGCGCTCC AGCTTCTTCA CTCTACCACT GCAGATCTTT 
GAACTCGTTC ATTTTGAGTC ATACTCGCCC AGTTTCATCT CACTGTACTG TCAAGTGTCG 
GCTCTGCTGG CTCTGGAGTC TCTGATGTCT CAGCAGTCTC TGCGCGAGGC TTTCTCCGAG 
TCCGAGCTGT TCTCCGCCAA GCAGAAACAC CAGCAGGTGG AGGAGTATCT GCAGCATATG 
GAGGAGCTGT GGCGTGATGC GCGGTGGATG GTTATTCTCC AGTCTGCCCG ATACAAACAT 
CAGAGTTCTG GAGTGAGTTT GGCCTGGATC ATAGACTTCA CTAAGGAAAC CATCCCAGAG 
AAACCAGCCT CCACTTCCTC TCAGGTGGAC TTCCTGCCCT CGCCGAGCCC TTCGCCGGAG 
AACACACGCA AACACACCGA TTCTCATTTG TCGGATGAAG ACAACACCTC TGATGTGTTT 
CTGACCACCG ACAGCAGCGA GTACAGCTCC AGCGTGACCC CGAGCACCAG AGAGCTGGAT 
CTTCTTCCTC CGTCTCCCTC CAGCTTCAGA TCTCCTCCAT CATCCTCGTC TCAGTCCTCC 
CCGCGGTCTT CTCAGCCAGA CGTCCTGCAG CCAGTGAGCC GCCGTGAGCA CATCAGTGTG 
TTGGACAGCG ACTTCATCCT GCCGAGTCGT CAGATCGAGC TCTTACGTGT GACAGAGAAG 
AGGACAGCTC TCTGCGTTCG CACCAGCAGC CTGGAGTACC CGCCGACCAC GCCCACACAC 
ACCGCCTCCT CTAATCAGAT GCGCTGGACA CGCCCCTCCT CTTTTGATCT GTGTTTCTCT 
GCCCCCGAGC CCCGCCCCTC ACCAGTGCGC ACTCTATCAG AGGACAGCGG CGTGCAGAGA 
CTCTCCGACC CCGCGGCCGG GTCTCCGCAC AGACAGTGTC ACAGCACACT GAGAGTTTAC 
CCACAATACC GCACCGGTTT ACCCAAAGAC ACCAGCGTCA AATTGCGTGT GACTCCAGAG 
ACGTGTGCTG CAGAGCTGGT CCGTCTGGTG GTTCAGGAGA TGAACGCAGT GTGTCGTCAC 
CTCCAGAAGG CCACTCATCA GTGCTCGTGT GCAGCCGATC AGTGTGTGCC CTCTGACGTG 
TGTGTGTACA GCGGCGAGCA GCTCCAGCAT TTCGGCCTGG TTCTGCTGGT GGAGGGACGA 
GAAAAGTGGC TGCAGGACGA TTTCTGCCCG CTTACACTGC AAAACCCCTG GACACGCGGG 
AAACTCTGCG TCCGCTTCAA GGAGTATTCA CCACTCGCAC TGCAGTACAG CAGAGCCACC 
ACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326079.1
CDS	197..4225
Translation	MASFGKLTDYFNRRRSRDELMARRDARRSGSYCCTVAEARSLERRLQRPLLAKSRTLPSIPQSPAAVRVQQTESDCERQQRPRVPSSPGGLEACTIPPAGEMWRLEDESEADMAPGAVDLRPRSQSPFSHFRRRAAYLRKSISADDHLVDSEFQSIQTEDRSSADPKAKLKRKFSLGSIDRKGTRQRRTESGIVKLTQRLSLKDKHCSKEDRSLQSRPPAYRRRSLSVDWAESKMTQQIPDPHPLIARKYSTSCSSPPSAARRLYRNLSGKFRTANLVLDDTTLPGQSDKEQSHKTTMFQGNEALFEAVEHQELDVVESLLNTFSLEELDLNTPNSEGLLPLDIAIMTNNVPMAKLLLRAGAKESPHFLSLEGRAAHLASLVQEAEHRIAELVAQVTGEEPGEREESNRERQLKSWEWKLRLYKRMQTGYTHASPPEPPSDVHLCVSSGTSLRVYFQEPLCHNSAVITKYRVSWSSVSSFSPLLGETVIEDVTQLQYDITGLAAGTGCYVQVSAYNMKGWSSPQISEPACATPSSWREVDGLPPRQQGLKEALEQLLGQIKGAHRHCACHEQIKAPSNTRKHSVSKSLRHLFQPTSKFVKNLKRGLYLACIFYQDENVLVTPEDQLPVVEVDDSFSCHAQDLLWFTKVCHLWEELSWMQQCISPAHACSSCTLQTRLKLLQAAAQLQALLGTVDLGQVYFEPIKDKHGNAVLVLLKDMSACAPLEGLRWIPVSKFQLQRRSSSSSSEEPSALETLLSTLHEKLSYHRRSRKCLPPGLYLGYLKLYTSVDQIRVLVSHKHPNVLCHVKIRDNGHVSREEWQWLQSLTSLEDSAKVCEDAQSAPHRLLLDLRTAAKELLAYVNIPNQQAQDFRVYVKEVVELGEGVSFLLLLPPCEEVCSPPGQNHLCSPRSSFFTLPLQIFELVHFESYSPSFISLYCQVSALLALESLMSQQSLREAFSESELFSAKQKHQQVEEYLQHMEELWRDARWMVILQSARYKHQSSGVSLAWIIDFTKETIPEKPASTSSQVDFLPSPSPSPENTRKHTDSHLSDEDNTSDVFLTTDSSEYSSSVTPSTRELDLLPPSPSSFRSPPSSSSQSSPRSSQPDVLQPVSRREHISVLDSDFILPSRQIELLRVTEKRTALCVRTSSLEYPPTTPTHTASSNQMRWTRPSSFDLCFSAPEPRPSPVRTLSEDSGVQRLSDPAAGSPHRQCHSTLRVYPQYRTGLPKDTSVKLRVTPETCAAELVRLVVQEMNAVCRHLQKATHQCSCAADQCVPSDVCVYSGEQLQHFGLVLLVEGREKWLQDDFCPLTLQNPWTRGKLCVRFKEYSPLALQYSRATTV

Target ORF information:

RefSeq Version	XM_021470404.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470404.1

ORF Insert Sequence:

ATGGCAAGTT TTGGGAAACT TACGGATTAC TTTAACCGTC GGCGTTCCAG GGATGAGCTC 
ATGGCTCGGA GAGATGCGCG ACGCAGCGGC AGTTACTGCT GTACGGTGGC AGAAGCCAGG 
TCTCTAGAGA GACGCCTGCA GAGGCCTCTG TTGGCCAAAT CTAGAACATT ACCCAGCATC 
CCTCAGTCCC CTGCTGCAGT CCGTGTGCAG CAGACGGAGA GTGATTGTGA GCGCCAACAA 
CGGCCTCGTG TTCCTTCTAG CCCTGGGGGT CTTGAAGCCT GCACCATTCC ACCTGCAGGT 
GAGATGTGGA GGCTGGAGGA TGAGAGCGAG GCAGACATGG CTCCAGGTGC CGTTGACCTG 
CGTCCTCGCT CTCAGTCACC GTTCTCACAT TTCAGACGGC GAGCGGCGTA TCTGAGGAAG 
AGCATCTCAG CGGACGATCA TCTAGTGGAT TCAGAGTTCC AGAGCATCCA GACAGAGGAC 
AGGAGCTCAG CTGATCCCAA AGCTAAACTC AAGAGGAAGT TTAGTCTGGG CTCCATCGAC 
AGGAAAGGAA CGCGACAGAG AAGGACTGAA TCTGGGATTG TGAAACTGAC CCAGCGACTC 
TCTCTGAAGG ACAAACACTG CAGTAAAGAG GACAGGAGTC TGCAGTCCAG ACCTCCGGCC 
TACAGGAGGC GCTCTCTCAG CGTGGACTGG GCCGAATCCA AAATGACTCA ACAGATTCCA 
GACCCTCATC CTCTGATTGC ACGGAAATAC AGCACATCAT GTTCGTCTCC ACCGAGCGCA 
GCGCGGCGTC TCTACAGGAA CCTGTCGGGG AAATTCAGAA CAGCAAACCT GGTTTTGGAT 
GATACGACAC TCCCGGGACA GTCGGACAAA GAGCAGTCAC ACAAAACCAC CATGTTCCAG 
GGTAACGAGG CTCTGTTTGA AGCGGTGGAG CATCAGGAGC TGGACGTGGT GGAGTCTCTG 
CTGAACACCT TCAGTCTGGA GGAGCTGGAC TTGAACACAC CCAACAGTGA AGGCCTGCTG 
CCGCTCGACA TCGCCATCAT GACCAACAAC GTGCCCATGG CCAAACTGCT GCTGAGAGCC 
GGGGCCAAAG AGAGTCCCCA CTTTTTAAGT CTTGAAGGTC GAGCTGCTCA CCTGGCTTCT 
CTGGTGCAAG AGGCAGAGCA TCGTATAGCA GAGCTGGTTG CCCAGGTGAC AGGGGAGGAG 
CCTGGAGAGA GGGAGGAGTC AAACAGAGAG AGACAGCTGA AATCATGGGA ATGGAAGCTC 
CGCCTCTATA AGCGCATGCA GACGGGATAC ACACATGCCA GTCCTCCCGA ACCTCCCAGT 
GACGTACATC TGTGTGTCAG CAGTGGCACC AGTCTCAGAG TTTACTTCCA AGAGCCTCTA 
TGTCACAATT CGGCTGTTAT AACCAAATAC CGCGTGAGCT GGAGCAGTGT TTCTTCATTT 
AGCCCTTTGC TGGGTGAGAC GGTCATTGAA GACGTGACTC AACTCCAGTA TGACATCACA 
GGACTCGCTG CGGGCACAGG CTGTTATGTC CAGGTCTCAG CATATAATAT GAAGGGATGG 
AGTTCACCTC AGATATCAGA ACCTGCTTGT GCTACACCCT CCAGTTGGAG GGAGGTGGAT 
GGTCTTCCCC CCCGCCAGCA GGGTCTGAAA GAGGCGTTAG AGCAGCTCCT GGGGCAAATT 
AAAGGGGCGC ACCGTCACTG CGCATGCCAC GAACAAATCA AAGCTCCGTC GAACACCAGA 
AAACATTCTG TCTCTAAAAG TCTGCGGCAT CTCTTCCAGC CCACCAGCAA ATTTGTCAAA 
AACCTGAAAA GAGGTCTTTA TCTAGCCTGT ATATTTTATC AAGATGAAAA CGTTCTGGTG 
ACTCCAGAGG ATCAGCTGCC GGTTGTAGAG GTGGACGACT CCTTCAGCTG TCACGCACAG 
GATTTACTCT GGTTCACAAA GGTGTGTCAC CTGTGGGAGG AGTTGAGCTG GATGCAGCAG 
TGTATCAGTC CTGCTCACGC CTGCAGCTCC TGCACACTTC AGACACGCCT CAAACTGCTG 
CAGGCAGCGG CACAACTACA GGCTCTGCTG GGCACGGTGG ATCTGGGACA GGTGTATTTC 
GAGCCCATTA AAGACAAGCA CGGTAATGCA GTGCTGGTGC TGCTGAAGGA CATGAGTGCG 
TGTGCGCCGC TGGAGGGACT GCGCTGGATT CCTGTCAGTA AATTCCAGCT TCAGCGAAGG 
TCTTCCTCGT CTTCATCAGA AGAGCCCAGC GCACTGGAGA CGCTCCTCTC CACACTGCAC 
GAGAAGCTGT CGTATCACCG GCGCAGCAGG AAGTGTCTGC CTCCAGGTCT GTATCTGGGT 
TACTTGAAGC TCTACACCTC AGTGGACCAG ATCCGAGTCC TGGTGTCACA CAAACACCCA 
AACGTGCTCT GCCACGTCAA GATACGAGAC AACGGCCACG TCTCCAGGGA GGAATGGCAG 
TGGCTTCAGT CACTGACTTC ACTGGAGGAC AGCGCGAAGG TGTGTGAGGA TGCTCAGAGT 
GCTCCGCATC GCCTCCTGCT GGACTTACGA ACCGCCGCCA AAGAGTTACT CGCATACGTC 
AACATTCCCA ACCAGCAGGC GCAGGACTTC CGTGTGTATG TGAAGGAGGT GGTGGAGCTG 
GGTGAAGGAG TCTCATTCCT GCTACTGCTG CCGCCCTGTG AGGAGGTGTG TTCACCACCG 
GGACAAAACC ACCTGTGCTC ACCGCGCTCC AGCTTCTTCA CTCTACCACT GCAGATCTTT 
GAACTCGTTC ATTTTGAGTC ATACTCGCCC AGTTTCATCT CACTGTACTG TCAAGTGTCG 
GCTCTGCTGG CTCTGGAGTC TCTGATGTCT CAGCAGTCTC TGCGCGAGGC TTTCTCCGAG 
TCCGAGCTGT TCTCCGCCAA GCAGAAACAC CAGCAGGTGG AGGAGTATCT GCAGCATATG 
GAGGAGCTGT GGCGTGATGC GCGGTGGATG GTTATTCTCC AGTCTGCCCG ATACAAACAT 
CAGAGTTCTG GAGTGAGTTT GGCCTGGATC ATAGACTTCA CTAAGGAAAC CATCCCAGAG 
AAACCAGCCT CCACTTCCTC TCAGGTGGAC TTCCTGCCCT CGCCGAGCCC TTCGCCGGAG 
AACACACGCA AACACACCGA TTCTCATTTG TCGGATGAAG ACAACACCTC TGATGTGTTT 
CTGACCACCG ACAGCAGCGA GTACAGCTCC AGCGTGACCC CGAGCACCAG AGAGCTGGAT 
CTTCTTCCTC CGTCTCCCTC CAGCTTCAGA TCTCCTCCAT CATCCTCGTC TCAGTCCTCC 
CCGCGGTCTT CTCAGCCAGA CGTCCTGCAG CCAGTGAGCC GCCGTGAGCA CATCAGTGTG 
TTGGACAGCG ACTTCATCCT GCCGAGTCGT CAGATCGAGC TCTTACGTGT GACAGAGAAG 
AGGACAGCTC TCTGCGTTCG CACCAGCAGC CTGGAGTACC CGCCGACCAC GCCCACACAC 
ACCGCCTCCT CTAATCAGAT GCGCTGGACA CGCCCCTCCT CTTTTGATCT GTGTTTCTCT 
GCCCCCGAGC CCCGCCCCTC ACCAGTGCGC ACTCTATCAG AGGACAGCGG CGTGCAGAGA 
CTCTCCGACC CCGCGGCCGG GTCTCCGCAC AGACAGTGTC ACAGCACACT GAGAGTTTAC 
CCACAATACC GCACCGGTTT ACCCAAAGAC ACCAGCGTCA AATTGCGTGT GACTCCAGAG 
ACGTGTGCTG CAGAGCTGGT CCGTCTGGTG GTTCAGGAGA TGAACGCAGT GTGTCGTCAC 
CTCCAGAAGG CCACTCATCA GTGCTCGTGT GCAGCCGATC AGTGTGTGCC CTCTGACGTG 
TGTGTGTACA GCGGCGAGCA GCTCCAGCAT TTCGGCCTGG TTCTGCTGGT GGAGGGACGA 
GAAAAGTGGC TGCAGGACGA TTTCTGCCCG CTTACACTGC AAAACCCCTG GACACGCGGG 
AAACTCTGCG TCCGCTTCAA GGAGTATTCA CCACTCGCAC TGCAGTACAG CAGAGCCACC 
ACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100535772 ( XM_021470405.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326080.1 Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa28886
Clone ID Related Accession (Same CDS sequence)	XM_021470405.1
Accession Version	XM_021470405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAAC AGATTCCAGA CCCTCATCCT CTGATTGCAC GGAAATACAG CACATCATGT 
TCGTCTCCAC CGAGCGCAGC GCGGCGTCTC TACAGGAACC TGTCGGGGAA ATTCAGAACA 
GCAAACCTGG TTTTGGATGA TACGACACTC CCGGGACAGT CGGACAAAGA GCAGTCACAC 
AAAACCACCA TGTTCCAGGG TAACGAGGCT CTGTTTGAAG CGGTGGAGCA TCAGGAGCTG 
GACGTGGTGG AGTCTCTGCT GAACACCTTC AGTCTGGAGG AGCTGGACTT GAACACACCC 
AACAGTGAAG GCCTGCTGCC GCTCGACATC GCCATCATGA CCAACAACGT GCCCATGGCC 
AAACTGCTGC TGAGAGCCGG GGCCAAAGAG AGTCCCCACT TTTTAAGTCT TGAAGGTCGA 
GCTGCTCACC TGGCTTCTCT GGTGCAAGAG GCAGAGCATC GTATAGCAGA GCTGGTTGCC 
CAGGTGACAG GGGAGGAGCC TGGAGAGAGG GAGGAGTCAA ACAGAGAGAG ACAGCTGAAA 
TCATGGGAAT GGAAGCTCCG CCTCTATAAG CGCATGCAGA CGGGATACAC ACATGCCAGT 
CCTCCCGAAC CTCCCAGTGA CGTACATCTG TGTGTCAGCA GTGGCACCAG TCTCAGAGTT 
TACTTCCAAG AGCCTCTATG TCACAATTCG GCTGTTATAA CCAAATACCG CGTGAGCTGG 
AGCAGTGTTT CTTCATTTAG CCCTTTGCTG GGTGAGACGG TCATTGAAGA CGTGACTCAA 
CTCCAGTATG ACATCACAGG ACTCGCTGCG GGCACAGGCT GTTATGTCCA GGTCTCAGCA 
TATAATATGA AGGGATGGAG TTCACCTCAG ATATCAGAAC CTGCTTGTGC TACACCCTCC 
AGTTGGAGGG AGGTGGATGG TCTTCCCCCC CGCCAGCAGG GTCTGAAAGA GGCGTTAGAG 
CAGCTCCTGG GGCAAATTAA AGGGGCGCAC CGTCACTGCG CATGCCACGA ACAAATCAAA 
GCTCCGTCGA ACACCAGAAA ACATTCTGTC TCTAAAAGTC TGCGGCATCT CTTCCAGCCC 
ACCAGCAAAT TTGTCAAAAA CCTGAAAAGA GGTCTTTATC TAGCCTGTAT ATTTTATCAA 
GATGAAAACG TTCTGGTGAC TCCAGAGGAT CAGCTGCCGG TTGTAGAGGT GGACGACTCC 
TTCAGCTGTC ACGCACAGGA TTTACTCTGG TTCACAAAGG TGTGTCACCT GTGGGAGGAG 
TTGAGCTGGA TGCAGCAGTG TATCAGTCCT GCTCACGCCT GCAGCTCCTG CACACTTCAG 
ACACGCCTCA AACTGCTGCA GGCAGCGGCA CAACTACAGG CTCTGCTGGG CACGGTGGAT 
CTGGGACAGG TGTATTTCGA GCCCATTAAA GACAAGCACG GTAATGCAGT GCTGGTGCTG 
CTGAAGGACA TGAGTGCGTG TGCGCCGCTG GAGGGACTGC GCTGGATTCC TGTCAGTAAA 
TTCCAGCTTC AGCGAAGGTC TTCCTCGTCT TCATCAGAAG AGCCCAGCGC ACTGGAGACG 
CTCCTCTCCA CACTGCACGA GAAGCTGTCG TATCACCGGC GCAGCAGGAA GTGTCTGCCT 
CCAGGTCTGT ATCTGGGTTA CTTGAAGCTC TACACCTCAG TGGACCAGAT CCGAGTCCTG 
GTGTCACACA AACACCCAAA CGTGCTCTGC CACGTCAAGA TACGAGACAA CGGCCACGTC 
TCCAGGGAGG AATGGCAGTG GCTTCAGTCA CTGACTTCAC TGGAGGACAG CGCGAAGGTG 
TGTGAGGATG CTCAGAGTGC TCCGCATCGC CTCCTGCTGG ACTTACGAAC CGCCGCCAAA 
GAGTTACTCG CATACGTCAA CATTCCCAAC CAGCAGGCGC AGGACTTCCG TGTGTATGTG 
AAGGAGGTGG TGGAGCTGGG TGAAGGAGTC TCATTCCTGC TACTGCTGCC GCCCTGTGAG 
GAGGTGTGTT CACCACCGGG ACAAAACCAC CTGTGCTCAC CGCGCTCCAG CTTCTTCACT 
CTACCACTGC AGATCTTTGA ACTCGTTCAT TTTGAGTCAT ACTCGCCCAG TTTCATCTCA 
CTGTACTGTC AAGTGTCGGC TCTGCTGGCT CTGGAGTCTC TGATGTCTCA GCAGTCTCTG 
CGCGAGGCTT TCTCCGAGTC CGAGCTGTTC TCCGCCAAGC AGAAACACCA GCAGGTGGAG 
GAGTATCTGC AGCATATGGA GGAGCTGTGG CGTGATGCGC GGTGGATGGT TATTCTCCAG 
TCTGCCCGAT ACAAACATCA GAGTTCTGGA GTGAGTTTGG CCTGGATCAT AGACTTCACT 
AAGGAAACCA TCCCAGAGAA ACCAGCCTCC ACTTCCTCTC AGGTGGACTT CCTGCCCTCG 
CCGAGCCCTT CGCCGGAGAA CACACGCAAA CACACCGATT CTCATTTGTC GGATGAAGAC 
AACACCTCTG ATGTGTTTCT GACCACCGAC AGCAGCGAGT ACAGCTCCAG CGTGACCCCG 
AGCACCAGAG AGCTGGATCT TCTTCCTCCG TCTCCCTCCA GCTTCAGATC TCCTCCATCA 
TCCTCGTCTC AGTCCTCCCC GCGGTCTTCT CAGCCAGACG TCCTGCAGCC AGTGAGCCGC 
CGTGAGCACA TCAGTGTGTT GGACAGCGAC TTCATCCTGC CGAGTCGTCA GATCGAGCTC 
TTACGTGTGA CAGAGAAGAG GACAGCTCTC TGCGTTCGCA CCAGCAGCCT GGAGTACCCG 
CCGACCACGC CCACACACAC CGCCTCCTCT AATCAGATGC GCTGGACACG CCCCTCCTCT 
TTTGATCTGT GTTTCTCTGC CCCCGAGCCC CGCCCCTCAC CAGTGCGCAC TCTATCAGAG 
GACAGCGGCG TGCAGAGACT CTCCGACCCC GCGGCCGGGT CTCCGCACAG ACAGTGTCAC 
AGCACACTGA GAGTTTACCC ACAATACCGC ACCGGTTTAC CCAAAGACAC CAGCGTCAAA 
TTGCGTGTGA CTCCAGAGAC GTGTGCTGCA GAGCTGGTCC GTCTGGTGGT TCAGGAGATG 
AACGCAGTGT GTCGTCACCT CCAGAAGGCC ACTCATCAGT GCTCGTGTGC AGCCGATCAG 
TGTGTGCCCT CTGACGTGTG TGTGTACAGC GGCGAGCAGC TCCAGCATTT CGGCCTGGTT 
CTGCTGGTGG AGGGACGAGA AAAGTGGCTG CAGGACGATT TCTGCCCGCT TACACTGCAA 
AACCCCTGGA CACGCGGGAA ACTCTGCGTC CGCTTCAAGG AGTATTCACC ACTCGCACTG 
CAGTACAGCA GAGCCACCAC TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326080.1
CDS	1059..4385
Translation	MTQQIPDPHPLIARKYSTSCSSPPSAARRLYRNLSGKFRTANLVLDDTTLPGQSDKEQSHKTTMFQGNEALFEAVEHQELDVVESLLNTFSLEELDLNTPNSEGLLPLDIAIMTNNVPMAKLLLRAGAKESPHFLSLEGRAAHLASLVQEAEHRIAELVAQVTGEEPGEREESNRERQLKSWEWKLRLYKRMQTGYTHASPPEPPSDVHLCVSSGTSLRVYFQEPLCHNSAVITKYRVSWSSVSSFSPLLGETVIEDVTQLQYDITGLAAGTGCYVQVSAYNMKGWSSPQISEPACATPSSWREVDGLPPRQQGLKEALEQLLGQIKGAHRHCACHEQIKAPSNTRKHSVSKSLRHLFQPTSKFVKNLKRGLYLACIFYQDENVLVTPEDQLPVVEVDDSFSCHAQDLLWFTKVCHLWEELSWMQQCISPAHACSSCTLQTRLKLLQAAAQLQALLGTVDLGQVYFEPIKDKHGNAVLVLLKDMSACAPLEGLRWIPVSKFQLQRRSSSSSSEEPSALETLLSTLHEKLSYHRRSRKCLPPGLYLGYLKLYTSVDQIRVLVSHKHPNVLCHVKIRDNGHVSREEWQWLQSLTSLEDSAKVCEDAQSAPHRLLLDLRTAAKELLAYVNIPNQQAQDFRVYVKEVVELGEGVSFLLLLPPCEEVCSPPGQNHLCSPRSSFFTLPLQIFELVHFESYSPSFISLYCQVSALLALESLMSQQSLREAFSESELFSAKQKHQQVEEYLQHMEELWRDARWMVILQSARYKHQSSGVSLAWIIDFTKETIPEKPASTSSQVDFLPSPSPSPENTRKHTDSHLSDEDNTSDVFLTTDSSEYSSSVTPSTRELDLLPPSPSSFRSPPSSSSQSSPRSSQPDVLQPVSRREHISVLDSDFILPSRQIELLRVTEKRTALCVRTSSLEYPPTTPTHTASSNQMRWTRPSSFDLCFSAPEPRPSPVRTLSEDSGVQRLSDPAAGSPHRQCHSTLRVYPQYRTGLPKDTSVKLRVTPETCAAELVRLVVQEMNAVCRHLQKATHQCSCAADQCVPSDVCVYSGEQLQHFGLVLLVEGREKWLQDDFCPLTLQNPWTRGKLCVRFKEYSPLALQYSRATTV

Target ORF information:

RefSeq Version	XM_021470405.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470405.1

ORF Insert Sequence:

ATGACTCAAC AGATTCCAGA CCCTCATCCT CTGATTGCAC GGAAATACAG CACATCATGT 
TCGTCTCCAC CGAGCGCAGC GCGGCGTCTC TACAGGAACC TGTCGGGGAA ATTCAGAACA 
GCAAACCTGG TTTTGGATGA TACGACACTC CCGGGACAGT CGGACAAAGA GCAGTCACAC 
AAAACCACCA TGTTCCAGGG TAACGAGGCT CTGTTTGAAG CGGTGGAGCA TCAGGAGCTG 
GACGTGGTGG AGTCTCTGCT GAACACCTTC AGTCTGGAGG AGCTGGACTT GAACACACCC 
AACAGTGAAG GCCTGCTGCC GCTCGACATC GCCATCATGA CCAACAACGT GCCCATGGCC 
AAACTGCTGC TGAGAGCCGG GGCCAAAGAG AGTCCCCACT TTTTAAGTCT TGAAGGTCGA 
GCTGCTCACC TGGCTTCTCT GGTGCAAGAG GCAGAGCATC GTATAGCAGA GCTGGTTGCC 
CAGGTGACAG GGGAGGAGCC TGGAGAGAGG GAGGAGTCAA ACAGAGAGAG ACAGCTGAAA 
TCATGGGAAT GGAAGCTCCG CCTCTATAAG CGCATGCAGA CGGGATACAC ACATGCCAGT 
CCTCCCGAAC CTCCCAGTGA CGTACATCTG TGTGTCAGCA GTGGCACCAG TCTCAGAGTT 
TACTTCCAAG AGCCTCTATG TCACAATTCG GCTGTTATAA CCAAATACCG CGTGAGCTGG 
AGCAGTGTTT CTTCATTTAG CCCTTTGCTG GGTGAGACGG TCATTGAAGA CGTGACTCAA 
CTCCAGTATG ACATCACAGG ACTCGCTGCG GGCACAGGCT GTTATGTCCA GGTCTCAGCA 
TATAATATGA AGGGATGGAG TTCACCTCAG ATATCAGAAC CTGCTTGTGC TACACCCTCC 
AGTTGGAGGG AGGTGGATGG TCTTCCCCCC CGCCAGCAGG GTCTGAAAGA GGCGTTAGAG 
CAGCTCCTGG GGCAAATTAA AGGGGCGCAC CGTCACTGCG CATGCCACGA ACAAATCAAA 
GCTCCGTCGA ACACCAGAAA ACATTCTGTC TCTAAAAGTC TGCGGCATCT CTTCCAGCCC 
ACCAGCAAAT TTGTCAAAAA CCTGAAAAGA GGTCTTTATC TAGCCTGTAT ATTTTATCAA 
GATGAAAACG TTCTGGTGAC TCCAGAGGAT CAGCTGCCGG TTGTAGAGGT GGACGACTCC 
TTCAGCTGTC ACGCACAGGA TTTACTCTGG TTCACAAAGG TGTGTCACCT GTGGGAGGAG 
TTGAGCTGGA TGCAGCAGTG TATCAGTCCT GCTCACGCCT GCAGCTCCTG CACACTTCAG 
ACACGCCTCA AACTGCTGCA GGCAGCGGCA CAACTACAGG CTCTGCTGGG CACGGTGGAT 
CTGGGACAGG TGTATTTCGA GCCCATTAAA GACAAGCACG GTAATGCAGT GCTGGTGCTG 
CTGAAGGACA TGAGTGCGTG TGCGCCGCTG GAGGGACTGC GCTGGATTCC TGTCAGTAAA 
TTCCAGCTTC AGCGAAGGTC TTCCTCGTCT TCATCAGAAG AGCCCAGCGC ACTGGAGACG 
CTCCTCTCCA CACTGCACGA GAAGCTGTCG TATCACCGGC GCAGCAGGAA GTGTCTGCCT 
CCAGGTCTGT ATCTGGGTTA CTTGAAGCTC TACACCTCAG TGGACCAGAT CCGAGTCCTG 
GTGTCACACA AACACCCAAA CGTGCTCTGC CACGTCAAGA TACGAGACAA CGGCCACGTC 
TCCAGGGAGG AATGGCAGTG GCTTCAGTCA CTGACTTCAC TGGAGGACAG CGCGAAGGTG 
TGTGAGGATG CTCAGAGTGC TCCGCATCGC CTCCTGCTGG ACTTACGAAC CGCCGCCAAA 
GAGTTACTCG CATACGTCAA CATTCCCAAC CAGCAGGCGC AGGACTTCCG TGTGTATGTG 
AAGGAGGTGG TGGAGCTGGG TGAAGGAGTC TCATTCCTGC TACTGCTGCC GCCCTGTGAG 
GAGGTGTGTT CACCACCGGG ACAAAACCAC CTGTGCTCAC CGCGCTCCAG CTTCTTCACT 
CTACCACTGC AGATCTTTGA ACTCGTTCAT TTTGAGTCAT ACTCGCCCAG TTTCATCTCA 
CTGTACTGTC AAGTGTCGGC TCTGCTGGCT CTGGAGTCTC TGATGTCTCA GCAGTCTCTG 
CGCGAGGCTT TCTCCGAGTC CGAGCTGTTC TCCGCCAAGC AGAAACACCA GCAGGTGGAG 
GAGTATCTGC AGCATATGGA GGAGCTGTGG CGTGATGCGC GGTGGATGGT TATTCTCCAG 
TCTGCCCGAT ACAAACATCA GAGTTCTGGA GTGAGTTTGG CCTGGATCAT AGACTTCACT 
AAGGAAACCA TCCCAGAGAA ACCAGCCTCC ACTTCCTCTC AGGTGGACTT CCTGCCCTCG 
CCGAGCCCTT CGCCGGAGAA CACACGCAAA CACACCGATT CTCATTTGTC GGATGAAGAC 
AACACCTCTG ATGTGTTTCT GACCACCGAC AGCAGCGAGT ACAGCTCCAG CGTGACCCCG 
AGCACCAGAG AGCTGGATCT TCTTCCTCCG TCTCCCTCCA GCTTCAGATC TCCTCCATCA 
TCCTCGTCTC AGTCCTCCCC GCGGTCTTCT CAGCCAGACG TCCTGCAGCC AGTGAGCCGC 
CGTGAGCACA TCAGTGTGTT GGACAGCGAC TTCATCCTGC CGAGTCGTCA GATCGAGCTC 
TTACGTGTGA CAGAGAAGAG GACAGCTCTC TGCGTTCGCA CCAGCAGCCT GGAGTACCCG 
CCGACCACGC CCACACACAC CGCCTCCTCT AATCAGATGC GCTGGACACG CCCCTCCTCT 
TTTGATCTGT GTTTCTCTGC CCCCGAGCCC CGCCCCTCAC CAGTGCGCAC TCTATCAGAG 
GACAGCGGCG TGCAGAGACT CTCCGACCCC GCGGCCGGGT CTCCGCACAG ACAGTGTCAC 
AGCACACTGA GAGTTTACCC ACAATACCGC ACCGGTTTAC CCAAAGACAC CAGCGTCAAA 
TTGCGTGTGA CTCCAGAGAC GTGTGCTGCA GAGCTGGTCC GTCTGGTGGT TCAGGAGATG 
AACGCAGTGT GTCGTCACCT CCAGAAGGCC ACTCATCAGT GCTCGTGTGC AGCCGATCAG 
TGTGTGCCCT CTGACGTGTG TGTGTACAGC GGCGAGCAGC TCCAGCATTT CGGCCTGGTT 
CTGCTGGTGG AGGGACGAGA AAAGTGGCTG CAGGACGATT TCTGCCCGCT TACACTGCAA 
AACCCCTGGA CACGCGGGAA ACTCTGCGTC CGCTTCAAGG AGTATTCACC ACTCGCACTG 
CAGTACAGCA GAGCCACCAC TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100535772 ( XM_017353975.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209464.1 Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X2, mRNA.

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CloneID	ODa57726
Clone ID Related Accession (Same CDS sequence)	XM_017353975.2 , XM_017353975.1
Accession Version	XM_017353975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGCGG GCTCTTATGC TGATGTATTC AGTACGGACA CACTGATGGA GTCTCTAGAG 
AGACGCCTGC AGAGGCCTCT GTTGGCCAAA TCTAGAACAT TACCCAGCAT CCCTCAGTCC 
CCTGCTGCAG TCCGTGTGCA GCAGACGGAG AGTGATTGTG AGCGCCAACA ACGGCCTCGT 
GTTCCTTCTA GCCCTGGGGG TCTTGAAGCC TGCACCATTC CACCTGCAGG TGAGATGTGG 
AGGCTGGAGG ATGAGAGCGA GGCAGACATG GCTCCAGGTG CCGTTGACCT GCGTCCTCGC 
TCTCAGTCAC CGTTCTCACA TTTCAGACGG CGAGCGGCGT ATCTGAGGAA GAGCATCTCA 
GCGGACGATC ATCTAGTGGA TTCAGAGTTC CAGAGCATCC AGACAGAGGA CAGGAGCTCA 
GCTGATCCCA AAGCTAAACT CAAGAGGAAG TTTAGTCTGG GCTCCATCGA CAGGAAAGGA 
ACGCGACAGA GAAGGACTGA ATCTGGGATT GTGAAACTGA CCCAGCGACT CTCTCTGAAG 
GACAAACACT GCAGTAAAGA GGACAGGAGT CTGCAGTCCA GACCTCCGGC CTACAGGAGG 
CGCTCTCTCA GCGTGGACTG GGCCGAATCC AAAATGACTC AACAGATTCC AGACCCTCAT 
CCTCTGATTG CACGGAAATA CAGCACATCA TGTTCGTCTC CACCGAGCGC AGCGCGGCGT 
CTCTACAGGA ACCTGTCGGG GAAATTCAGA ACAGCAAACC TGGTTTTGGA TGATACGACA 
CTCCCGGGAC AGTCGGACAA AGAGCAGTCA CACAAAACCA CCATGTTCCA GGGTAACGAG 
GCTCTGTTTG AAGCGGTGGA GCATCAGGAG CTGGACGTGG TGGAGTCTCT GCTGAACACC 
TTCAGTCTGG AGGAGCTGGA CTTGAACACA CCCAACAGTG AAGGCCTGCT GCCGCTCGAC 
ATCGCCATCA TGACCAACAA CGTGCCCATG GCCAAACTGC TGCTGAGAGC CGGGGCCAAA 
GAGAGTCCCC ACTTTTTAAG TCTTGAAGGT CGAGCTGCTC ACCTGGCTTC TCTGGTGCAA 
GAGGCAGAGC ATCGTATAGC AGAGCTGGTT GCCCAGGTGA CAGGGGAGGA GCCTGGAGAG 
AGGGAGGAGT CAAACAGAGA GAGACAGCTG AAATCATGGG AATGGAAGCT CCGCCTCTAT 
AAGCGCATGC AGACGGGATA CACACATGCC AGTCCTCCCG AACCTCCCAG TGACGTACAT 
CTGTGTGTCA GCAGTGGCAC CAGTCTCAGA GTTTACTTCC AAGAGCCTCT ATGTCACAAT 
TCGGCTGTTA TAACCAAATA CCGCGTGAGC TGGAGCAGTG TTTCTTCATT TAGCCCTTTG 
CTGGGTGAGA CGGTCATTGA AGACGTGACT CAACTCCAGT ATGACATCAC AGGACTCGCT 
GCGGGCACAG GCTGTTATGT CCAGGTCTCA GCATATAATA TGAAGGGATG GAGTTCACCT 
CAGATATCAG AACCTGCTTG TGCTACACCC TCCAGTTGGA GGGAGGTGGA TGGTCTTCCC 
CCCCGCCAGC AGGGTCTGAA AGAGGCGTTA GAGCAGCTCC TGGGGCAAAT TAAAGGGGCG 
CACCGTCACT GCGCATGCCA CGAACAAATC AAAGCTCCGT CGAACACCAG AAAACATTCT 
GTCTCTAAAA GTCTGCGGCA TCTCTTCCAG CCCACCAGCA AATTTGTCAA AAACCTGAAA 
AGAGGTCTTT ATCTAGCCTG TATATTTTAT CAAGATGAAA ACGTTCTGGT GACTCCAGAG 
GATCAGCTGC CGGTTGTAGA GGTGGACGAC TCCTTCAGCT GTCACGCACA GGATTTACTC 
TGGTTCACAA AGGTGTGTCA CCTGTGGGAG GAGTTGAGCT GGATGCAGCA GTGTATCAGT 
CCTGCTCACG CCTGCAGCTC CTGCACACTT CAGACACGCC TCAAACTGCT GCAGGCAGCG 
GCACAACTAC AGGCTCTGCT GGGCACGGTG GATCTGGGAC AGGTGTATTT CGAGCCCATT 
AAAGACAAGC ACGGTAATGC AGTGCTGGTG CTGCTGAAGG ACATGAGTGC GTGTGCGCCG 
CTGGAGGGAC TGCGCTGGAT TCCTGTCAGT AAATTCCAGC TTCAGCGAAG GTCTTCCTCG 
TCTTCATCAG AAGAGCCCAG CGCACTGGAG ACGCTCCTCT CCACACTGCA CGAGAAGCTG 
TCGTATCACC GGCGCAGCAG GAAGTGTCTG CCTCCAGGTC TGTATCTGGG TTACTTGAAG 
CTCTACACCT CAGTGGACCA GATCCGAGTC CTGGTGTCAC ACAAACACCC AAACGTGCTC 
TGCCACGTCA AGATACGAGA CAACGGCCAC GTCTCCAGGG AGGAATGGCA GTGGCTTCAG 
TCACTGACTT CACTGGAGGA CAGCGCGAAG GTGTGTGAGG ATGCTCAGAG TGCTCCGCAT 
CGCCTCCTGC TGGACTTACG AACCGCCGCC AAAGAGTTAC TCGCATACGT CAACATTCCC 
AACCAGCAGG CGCAGGACTT CCGTGTGTAT GTGAAGGAGG TGGTGGAGCT GGGTGAAGGA 
GTCTCATTCC TGCTACTGCT GCCGCCCTGT GAGGAGGTGT GTTCACCACC GGGACAAAAC 
CACCTGTGCT CACCGCGCTC CAGCTTCTTC ACTCTACCAC TGCAGATCTT TGAACTCGTT 
CATTTTGAGT CATACTCGCC CAGTTTCATC TCACTGTACT GTCAAGTGTC GGCTCTGCTG 
GCTCTGGAGT CTCTGATGTC TCAGCAGTCT CTGCGCGAGG CTTTCTCCGA GTCCGAGCTG 
TTCTCCGCCA AGCAGAAACA CCAGCAGGTG GAGGAGTATC TGCAGCATAT GGAGGAGCTG 
TGGCGTGATG CGCGGTGGAT GGTTATTCTC CAGTCTGCCC GATACAAACA TCAGAGTTCT 
GGAGTGAGTT TGGCCTGGAT CATAGACTTC ACTAAGGAAA CCATCCCAGA GAAACCAGCC 
TCCACTTCCT CTCAGGTGGA CTTCCTGCCC TCGCCGAGCC CTTCGCCGGA GAACACACGC 
AAACACACCG ATTCTCATTT GTCGGATGAA GACAACACCT CTGATGTGTT TCTGACCACC 
GACAGCAGCG AGTACAGCTC CAGCGTGACC CCGAGCACCA GAGAGCTGGA TCTTCTTCCT 
CCGTCTCCCT CCAGCTTCAG ATCTCCTCCA TCATCCTCGT CTCAGTCCTC CCCGCGGTCT 
TCTCAGCCAG ACGTCCTGCA GCCAGTGAGC CGCCGTGAGC ACATCAGTGT GTTGGACAGC 
GACTTCATCC TGCCGAGTCG TCAGATCGAG CTCTTACGTG TGACAGAGAA GAGGACAGCT 
CTCTGCGTTC GCACCAGCAG CCTGGAGTAC CCGCCGACCA CGCCCACACA CACCGCCTCC 
TCTAATCAGA TGCGCTGGAC ACGCCCCTCC TCTTTTGATC TGTGTTTCTC TGCCCCCGAG 
CCCCGCCCCT CACCAGTGCG CACTCTATCA GAGGACAGCG GCGTGCAGAG ACTCTCCGAC 
CCCGCGGCCG GGTCTCCGCA CAGACAGTGT CACAGCACAC TGAGAGTTTA CCCACAATAC 
CGCACCGGTT TACCCAAAGA CACCAGCGTC AAATTGCGTG TGACTCCAGA GACGTGTGCT 
GCAGAGCTGG TCCGTCTGGT GGTTCAGGAG ATGAACGCAG TGTGTCGTCA CCTCCAGAAG 
GCCACTCATC AGTGCTCGTG TGCAGCCGAT CAGTGTGTGC CCTCTGACGT GTGTGTGTAC 
AGCGGCGAGC AGCTCCAGCA TTTCGGCCTG GTTCTGCTGG TGGAGGGACG AGAAAAGTGG 
CTGCAGGACG ATTTCTGCCC GCTTACACTG CAAAACCCCT GGACACGCGG GAAACTCTGC 
GTCCGCTTCA AGGAGTATTC ACCACTCGCA CTGCAGTACA GCAGAGCCAC CACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209464.1
CDS	239..4198
Translation	MFAGSYADVFSTDTLMESLERRLQRPLLAKSRTLPSIPQSPAAVRVQQTESDCERQQRPRVPSSPGGLEACTIPPAGEMWRLEDESEADMAPGAVDLRPRSQSPFSHFRRRAAYLRKSISADDHLVDSEFQSIQTEDRSSADPKAKLKRKFSLGSIDRKGTRQRRTESGIVKLTQRLSLKDKHCSKEDRSLQSRPPAYRRRSLSVDWAESKMTQQIPDPHPLIARKYSTSCSSPPSAARRLYRNLSGKFRTANLVLDDTTLPGQSDKEQSHKTTMFQGNEALFEAVEHQELDVVESLLNTFSLEELDLNTPNSEGLLPLDIAIMTNNVPMAKLLLRAGAKESPHFLSLEGRAAHLASLVQEAEHRIAELVAQVTGEEPGEREESNRERQLKSWEWKLRLYKRMQTGYTHASPPEPPSDVHLCVSSGTSLRVYFQEPLCHNSAVITKYRVSWSSVSSFSPLLGETVIEDVTQLQYDITGLAAGTGCYVQVSAYNMKGWSSPQISEPACATPSSWREVDGLPPRQQGLKEALEQLLGQIKGAHRHCACHEQIKAPSNTRKHSVSKSLRHLFQPTSKFVKNLKRGLYLACIFYQDENVLVTPEDQLPVVEVDDSFSCHAQDLLWFTKVCHLWEELSWMQQCISPAHACSSCTLQTRLKLLQAAAQLQALLGTVDLGQVYFEPIKDKHGNAVLVLLKDMSACAPLEGLRWIPVSKFQLQRRSSSSSSEEPSALETLLSTLHEKLSYHRRSRKCLPPGLYLGYLKLYTSVDQIRVLVSHKHPNVLCHVKIRDNGHVSREEWQWLQSLTSLEDSAKVCEDAQSAPHRLLLDLRTAAKELLAYVNIPNQQAQDFRVYVKEVVELGEGVSFLLLLPPCEEVCSPPGQNHLCSPRSSFFTLPLQIFELVHFESYSPSFISLYCQVSALLALESLMSQQSLREAFSESELFSAKQKHQQVEEYLQHMEELWRDARWMVILQSARYKHQSSGVSLAWIIDFTKETIPEKPASTSSQVDFLPSPSPSPENTRKHTDSHLSDEDNTSDVFLTTDSSEYSSSVTPSTRELDLLPPSPSSFRSPPSSSSQSSPRSSQPDVLQPVSRREHISVLDSDFILPSRQIELLRVTEKRTALCVRTSSLEYPPTTPTHTASSNQMRWTRPSSFDLCFSAPEPRPSPVRTLSEDSGVQRLSDPAAGSPHRQCHSTLRVYPQYRTGLPKDTSVKLRVTPETCAAELVRLVVQEMNAVCRHLQKATHQCSCAADQCVPSDVCVYSGEQLQHFGLVLLVEGREKWLQDDFCPLTLQNPWTRGKLCVRFKEYSPLALQYSRATTV

Target ORF information:

RefSeq Version	XM_017353975.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353975.2

ORF Insert Sequence:

ATGTTCGCGG GCTCTTATGC TGATGTATTC AGTACGGACA CACTGATGGA GTCTCTAGAG 
AGACGCCTGC AGAGGCCTCT GTTGGCCAAA TCTAGAACAT TACCCAGCAT CCCTCAGTCC 
CCTGCTGCAG TCCGTGTGCA GCAGACGGAG AGTGATTGTG AGCGCCAACA ACGGCCTCGT 
GTTCCTTCTA GCCCTGGGGG TCTTGAAGCC TGCACCATTC CACCTGCAGG TGAGATGTGG 
AGGCTGGAGG ATGAGAGCGA GGCAGACATG GCTCCAGGTG CCGTTGACCT GCGTCCTCGC 
TCTCAGTCAC CGTTCTCACA TTTCAGACGG CGAGCGGCGT ATCTGAGGAA GAGCATCTCA 
GCGGACGATC ATCTAGTGGA TTCAGAGTTC CAGAGCATCC AGACAGAGGA CAGGAGCTCA 
GCTGATCCCA AAGCTAAACT CAAGAGGAAG TTTAGTCTGG GCTCCATCGA CAGGAAAGGA 
ACGCGACAGA GAAGGACTGA ATCTGGGATT GTGAAACTGA CCCAGCGACT CTCTCTGAAG 
GACAAACACT GCAGTAAAGA GGACAGGAGT CTGCAGTCCA GACCTCCGGC CTACAGGAGG 
CGCTCTCTCA GCGTGGACTG GGCCGAATCC AAAATGACTC AACAGATTCC AGACCCTCAT 
CCTCTGATTG CACGGAAATA CAGCACATCA TGTTCGTCTC CACCGAGCGC AGCGCGGCGT 
CTCTACAGGA ACCTGTCGGG GAAATTCAGA ACAGCAAACC TGGTTTTGGA TGATACGACA 
CTCCCGGGAC AGTCGGACAA AGAGCAGTCA CACAAAACCA CCATGTTCCA GGGTAACGAG 
GCTCTGTTTG AAGCGGTGGA GCATCAGGAG CTGGACGTGG TGGAGTCTCT GCTGAACACC 
TTCAGTCTGG AGGAGCTGGA CTTGAACACA CCCAACAGTG AAGGCCTGCT GCCGCTCGAC 
ATCGCCATCA TGACCAACAA CGTGCCCATG GCCAAACTGC TGCTGAGAGC CGGGGCCAAA 
GAGAGTCCCC ACTTTTTAAG TCTTGAAGGT CGAGCTGCTC ACCTGGCTTC TCTGGTGCAA 
GAGGCAGAGC ATCGTATAGC AGAGCTGGTT GCCCAGGTGA CAGGGGAGGA GCCTGGAGAG 
AGGGAGGAGT CAAACAGAGA GAGACAGCTG AAATCATGGG AATGGAAGCT CCGCCTCTAT 
AAGCGCATGC AGACGGGATA CACACATGCC AGTCCTCCCG AACCTCCCAG TGACGTACAT 
CTGTGTGTCA GCAGTGGCAC CAGTCTCAGA GTTTACTTCC AAGAGCCTCT ATGTCACAAT 
TCGGCTGTTA TAACCAAATA CCGCGTGAGC TGGAGCAGTG TTTCTTCATT TAGCCCTTTG 
CTGGGTGAGA CGGTCATTGA AGACGTGACT CAACTCCAGT ATGACATCAC AGGACTCGCT 
GCGGGCACAG GCTGTTATGT CCAGGTCTCA GCATATAATA TGAAGGGATG GAGTTCACCT 
CAGATATCAG AACCTGCTTG TGCTACACCC TCCAGTTGGA GGGAGGTGGA TGGTCTTCCC 
CCCCGCCAGC AGGGTCTGAA AGAGGCGTTA GAGCAGCTCC TGGGGCAAAT TAAAGGGGCG 
CACCGTCACT GCGCATGCCA CGAACAAATC AAAGCTCCGT CGAACACCAG AAAACATTCT 
GTCTCTAAAA GTCTGCGGCA TCTCTTCCAG CCCACCAGCA AATTTGTCAA AAACCTGAAA 
AGAGGTCTTT ATCTAGCCTG TATATTTTAT CAAGATGAAA ACGTTCTGGT GACTCCAGAG 
GATCAGCTGC CGGTTGTAGA GGTGGACGAC TCCTTCAGCT GTCACGCACA GGATTTACTC 
TGGTTCACAA AGGTGTGTCA CCTGTGGGAG GAGTTGAGCT GGATGCAGCA GTGTATCAGT 
CCTGCTCACG CCTGCAGCTC CTGCACACTT CAGACACGCC TCAAACTGCT GCAGGCAGCG 
GCACAACTAC AGGCTCTGCT GGGCACGGTG GATCTGGGAC AGGTGTATTT CGAGCCCATT 
AAAGACAAGC ACGGTAATGC AGTGCTGGTG CTGCTGAAGG ACATGAGTGC GTGTGCGCCG 
CTGGAGGGAC TGCGCTGGAT TCCTGTCAGT AAATTCCAGC TTCAGCGAAG GTCTTCCTCG 
TCTTCATCAG AAGAGCCCAG CGCACTGGAG ACGCTCCTCT CCACACTGCA CGAGAAGCTG 
TCGTATCACC GGCGCAGCAG GAAGTGTCTG CCTCCAGGTC TGTATCTGGG TTACTTGAAG 
CTCTACACCT CAGTGGACCA GATCCGAGTC CTGGTGTCAC ACAAACACCC AAACGTGCTC 
TGCCACGTCA AGATACGAGA CAACGGCCAC GTCTCCAGGG AGGAATGGCA GTGGCTTCAG 
TCACTGACTT CACTGGAGGA CAGCGCGAAG GTGTGTGAGG ATGCTCAGAG TGCTCCGCAT 
CGCCTCCTGC TGGACTTACG AACCGCCGCC AAAGAGTTAC TCGCATACGT CAACATTCCC 
AACCAGCAGG CGCAGGACTT CCGTGTGTAT GTGAAGGAGG TGGTGGAGCT GGGTGAAGGA 
GTCTCATTCC TGCTACTGCT GCCGCCCTGT GAGGAGGTGT GTTCACCACC GGGACAAAAC 
CACCTGTGCT CACCGCGCTC CAGCTTCTTC ACTCTACCAC TGCAGATCTT TGAACTCGTT 
CATTTTGAGT CATACTCGCC CAGTTTCATC TCACTGTACT GTCAAGTGTC GGCTCTGCTG 
GCTCTGGAGT CTCTGATGTC TCAGCAGTCT CTGCGCGAGG CTTTCTCCGA GTCCGAGCTG 
TTCTCCGCCA AGCAGAAACA CCAGCAGGTG GAGGAGTATC TGCAGCATAT GGAGGAGCTG 
TGGCGTGATG CGCGGTGGAT GGTTATTCTC CAGTCTGCCC GATACAAACA TCAGAGTTCT 
GGAGTGAGTT TGGCCTGGAT CATAGACTTC ACTAAGGAAA CCATCCCAGA GAAACCAGCC 
TCCACTTCCT CTCAGGTGGA CTTCCTGCCC TCGCCGAGCC CTTCGCCGGA GAACACACGC 
AAACACACCG ATTCTCATTT GTCGGATGAA GACAACACCT CTGATGTGTT TCTGACCACC 
GACAGCAGCG AGTACAGCTC CAGCGTGACC CCGAGCACCA GAGAGCTGGA TCTTCTTCCT 
CCGTCTCCCT CCAGCTTCAG ATCTCCTCCA TCATCCTCGT CTCAGTCCTC CCCGCGGTCT 
TCTCAGCCAG ACGTCCTGCA GCCAGTGAGC CGCCGTGAGC ACATCAGTGT GTTGGACAGC 
GACTTCATCC TGCCGAGTCG TCAGATCGAG CTCTTACGTG TGACAGAGAA GAGGACAGCT 
CTCTGCGTTC GCACCAGCAG CCTGGAGTAC CCGCCGACCA CGCCCACACA CACCGCCTCC 
TCTAATCAGA TGCGCTGGAC ACGCCCCTCC TCTTTTGATC TGTGTTTCTC TGCCCCCGAG 
CCCCGCCCCT CACCAGTGCG CACTCTATCA GAGGACAGCG GCGTGCAGAG ACTCTCCGAC 
CCCGCGGCCG GGTCTCCGCA CAGACAGTGT CACAGCACAC TGAGAGTTTA CCCACAATAC 
CGCACCGGTT TACCCAAAGA CACCAGCGTC AAATTGCGTG TGACTCCAGA GACGTGTGCT 
GCAGAGCTGG TCCGTCTGGT GGTTCAGGAG ATGAACGCAG TGTGTCGTCA CCTCCAGAAG 
GCCACTCATC AGTGCTCGTG TGCAGCCGAT CAGTGTGTGC CCTCTGACGT GTGTGTGTAC 
AGCGGCGAGC AGCTCCAGCA TTTCGGCCTG GTTCTGCTGG TGGAGGGACG AGAAAAGTGG 
CTGCAGGACG ATTTCTGCCC GCTTACACTG CAAAACCCCT GGACACGCGG GAAACTCTGC 
GTCCGCTTCA AGGAGTATTC ACCACTCGCA CTGCAGTACA GCAGAGCCAC CACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100535772 ( XM_017353975.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209464.1 Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), mRNA.

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CloneID	ODa57726
Clone ID Related Accession (Same CDS sequence)	XM_017353975.2 , XM_017353975.1
Accession Version	XM_017353975.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat and fibronectin type-III domain-containing protein 1-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGCGG GCTCTTATGC TGATGTATTC AGTACGGACA CACTGATGGA GTCTCTAGAG 
AGACGCCTGC AGAGGCCTCT GTTGGCCAAA TCTAGAACAT TACCCAGCAT CCCTCAGTCC 
CCTGCTGCAG TCCGTGTGCA GCAGACGGAG AGTGATTGTG AGCGCCAACA ACGGCCTCGT 
GTTCCTTCTA GCCCTGGGGG TCTTGAAGCC TGCACCATTC CACCTGCAGG TGAGATGTGG 
AGGCTGGAGG ATGAGAGCGA GGCAGACATG GCTCCAGGTG CCGTTGACCT GCGTCCTCGC 
TCTCAGTCAC CGTTCTCACA TTTCAGACGG CGAGCGGCGT ATCTGAGGAA GAGCATCTCA 
GCGGACGATC ATCTAGTGGA TTCAGAGTTC CAGAGCATCC AGACAGAGGA CAGGAGCTCA 
GCTGATCCCA AAGCTAAACT CAAGAGGAAG TTTAGTCTGG GCTCCATCGA CAGGAAAGGA 
ACGCGACAGA GAAGGACTGA ATCTGGGATT GTGAAACTGA CCCAGCGACT CTCTCTGAAG 
GACAAACACT GCAGTAAAGA GGACAGGAGT CTGCAGTCCA GACCTCCGGC CTACAGGAGG 
CGCTCTCTCA GCGTGGACTG GGCCGAATCC AAAATGACTC AACAGATTCC AGACCCTCAT 
CCTCTGATTG CACGGAAATA CAGCACATCA TGTTCGTCTC CACCGAGCGC AGCGCGGCGT 
CTCTACAGGA ACCTGTCGGG GAAATTCAGA ACAGCAAACC TGGTTTTGGA TGATACGACA 
CTCCCGGGAC AGTCGGACAA AGAGCAGTCA CACAAAACCA CCATGTTCCA GGGTAACGAG 
GCTCTGTTTG AAGCGGTGGA GCATCAGGAG CTGGACGTGG TGGAGTCTCT GCTGAACACC 
TTCAGTCTGG AGGAGCTGGA CTTGAACACA CCCAACAGTG AAGGCCTGCT GCCGCTCGAC 
ATCGCCATCA TGACCAACAA CGTGCCCATG GCCAAACTGC TGCTGAGAGC CGGGGCCAAA 
GAGAGTCCCC ACTTTTTAAG TCTTGAAGGT CGAGCTGCTC ACCTGGCTTC TCTGGTGCAA 
GAGGCAGAGC ATCGTATAGC AGAGCTGGTT GCCCAGGTGA CAGGGGAGGA GCCTGGAGAG 
AGGGAGGAGT CAAACAGAGA GAGACAGCTG AAATCATGGG AATGGAAGCT CCGCCTCTAT 
AAGCGCATGC AGACGGGATA CACACATGCC AGTCCTCCCG AACCTCCCAG TGACGTACAT 
CTGTGTGTCA GCAGTGGCAC CAGTCTCAGA GTTTACTTCC AAGAGCCTCT ATGTCACAAT 
TCGGCTGTTA TAACCAAATA CCGCGTGAGC TGGAGCAGTG TTTCTTCATT TAGCCCTTTG 
CTGGGTGAGA CGGTCATTGA AGACGTGACT CAACTCCAGT ATGACATCAC AGGACTCGCT 
GCGGGCACAG GCTGTTATGT CCAGGTCTCA GCATATAATA TGAAGGGATG GAGTTCACCT 
CAGATATCAG AACCTGCTTG TGCTACACCC TCCAGTTGGA GGGAGGTGGA TGGTCTTCCC 
CCCCGCCAGC AGGGTCTGAA AGAGGCGTTA GAGCAGCTCC TGGGGCAAAT TAAAGGGGCG 
CACCGTCACT GCGCATGCCA CGAACAAATC AAAGCTCCGT CGAACACCAG AAAACATTCT 
GTCTCTAAAA GTCTGCGGCA TCTCTTCCAG CCCACCAGCA AATTTGTCAA AAACCTGAAA 
AGAGGTCTTT ATCTAGCCTG TATATTTTAT CAAGATGAAA ACGTTCTGGT GACTCCAGAG 
GATCAGCTGC CGGTTGTAGA GGTGGACGAC TCCTTCAGCT GTCACGCACA GGATTTACTC 
TGGTTCACAA AGGTGTGTCA CCTGTGGGAG GAGTTGAGCT GGATGCAGCA GTGTATCAGT 
CCTGCTCACG CCTGCAGCTC CTGCACACTT CAGACACGCC TCAAACTGCT GCAGGCAGCG 
GCACAACTAC AGGCTCTGCT GGGCACGGTG GATCTGGGAC AGGTGTATTT CGAGCCCATT 
AAAGACAAGC ACGGTAATGC AGTGCTGGTG CTGCTGAAGG ACATGAGTGC GTGTGCGCCG 
CTGGAGGGAC TGCGCTGGAT TCCTGTCAGT AAATTCCAGC TTCAGCGAAG GTCTTCCTCG 
TCTTCATCAG AAGAGCCCAG CGCACTGGAG ACGCTCCTCT CCACACTGCA CGAGAAGCTG 
TCGTATCACC GGCGCAGCAG GAAGTGTCTG CCTCCAGGTC TGTATCTGGG TTACTTGAAG 
CTCTACACCT CAGTGGACCA GATCCGAGTC CTGGTGTCAC ACAAACACCC AAACGTGCTC 
TGCCACGTCA AGATACGAGA CAACGGCCAC GTCTCCAGGG AGGAATGGCA GTGGCTTCAG 
TCACTGACTT CACTGGAGGA CAGCGCGAAG GTGTGTGAGG ATGCTCAGAG TGCTCCGCAT 
CGCCTCCTGC TGGACTTACG AACCGCCGCC AAAGAGTTAC TCGCATACGT CAACATTCCC 
AACCAGCAGG CGCAGGACTT CCGTGTGTAT GTGAAGGAGG TGGTGGAGCT GGGTGAAGGA 
GTCTCATTCC TGCTACTGCT GCCGCCCTGT GAGGAGGTGT GTTCACCACC GGGACAAAAC 
CACCTGTGCT CACCGCGCTC CAGCTTCTTC ACTCTACCAC TGCAGATCTT TGAACTCGTT 
CATTTTGAGT CATACTCGCC CAGTTTCATC TCACTGTACT GTCAAGTGTC GGCTCTGCTG 
GCTCTGGAGT CTCTGATGTC TCAGCAGTCT CTGCGCGAGG CTTTCTCCGA GTCCGAGCTG 
TTCTCCGCCA AGCAGAAACA CCAGCAGGTG GAGGAGTATC TGCAGCATAT GGAGGAGCTG 
TGGCGTGATG CGCGGTGGAT GGTTATTCTC CAGTCTGCCC GATACAAACA TCAGAGTTCT 
GGAGTGAGTT TGGCCTGGAT CATAGACTTC ACTAAGGAAA CCATCCCAGA GAAACCAGCC 
TCCACTTCCT CTCAGGTGGA CTTCCTGCCC TCGCCGAGCC CTTCGCCGGA GAACACACGC 
AAACACACCG ATTCTCATTT GTCGGATGAA GACAACACCT CTGATGTGTT TCTGACCACC 
GACAGCAGCG AGTACAGCTC CAGCGTGACC CCGAGCACCA GAGAGCTGGA TCTTCTTCCT 
CCGTCTCCCT CCAGCTTCAG ATCTCCTCCA TCATCCTCGT CTCAGTCCTC CCCGCGGTCT 
TCTCAGCCAG ACGTCCTGCA GCCAGTGAGC CGCCGTGAGC ACATCAGTGT GTTGGACAGC 
GACTTCATCC TGCCGAGTCG TCAGATCGAG CTCTTACGTG TGACAGAGAA GAGGACAGCT 
CTCTGCGTTC GCACCAGCAG CCTGGAGTAC CCGCCGACCA CGCCCACACA CACCGCCTCC 
TCTAATCAGA TGCGCTGGAC ACGCCCCTCC TCTTTTGATC TGTGTTTCTC TGCCCCCGAG 
CCCCGCCCCT CACCAGTGCG CACTCTATCA GAGGACAGCG GCGTGCAGAG ACTCTCCGAC 
CCCGCGGCCG GGTCTCCGCA CAGACAGTGT CACAGCACAC TGAGAGTTTA CCCACAATAC 
CGCACCGGTT TACCCAAAGA CACCAGCGTC AAATTGCGTG TGACTCCAGA GACGTGTGCT 
GCAGAGCTGG TCCGTCTGGT GGTTCAGGAG ATGAACGCAG TGTGTCGTCA CCTCCAGAAG 
GCCACTCATC AGTGCTCGTG TGCAGCCGAT CAGTGTGTGC CCTCTGACGT GTGTGTGTAC 
AGCGGCGAGC AGCTCCAGCA TTTCGGCCTG GTTCTGCTGG TGGAGGGACG AGAAAAGTGG 
CTGCAGGACG ATTTCTGCCC GCTTACACTG CAAAACCCCT GGACACGCGG GAAACTCTGC 
GTCCGCTTCA AGGAGTATTC ACCACTCGCA CTGCAGTACA GCAGAGCCAC CACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209464.1
CDS	214..4173
Translation	MFAGSYADVFSTDTLMESLERRLQRPLLAKSRTLPSIPQSPAAVRVQQTESDCERQQRPRVPSSPGGLEACTIPPAGEMWRLEDESEADMAPGAVDLRPRSQSPFSHFRRRAAYLRKSISADDHLVDSEFQSIQTEDRSSADPKAKLKRKFSLGSIDRKGTRQRRTESGIVKLTQRLSLKDKHCSKEDRSLQSRPPAYRRRSLSVDWAESKMTQQIPDPHPLIARKYSTSCSSPPSAARRLYRNLSGKFRTANLVLDDTTLPGQSDKEQSHKTTMFQGNEALFEAVEHQELDVVESLLNTFSLEELDLNTPNSEGLLPLDIAIMTNNVPMAKLLLRAGAKESPHFLSLEGRAAHLASLVQEAEHRIAELVAQVTGEEPGEREESNRERQLKSWEWKLRLYKRMQTGYTHASPPEPPSDVHLCVSSGTSLRVYFQEPLCHNSAVITKYRVSWSSVSSFSPLLGETVIEDVTQLQYDITGLAAGTGCYVQVSAYNMKGWSSPQISEPACATPSSWREVDGLPPRQQGLKEALEQLLGQIKGAHRHCACHEQIKAPSNTRKHSVSKSLRHLFQPTSKFVKNLKRGLYLACIFYQDENVLVTPEDQLPVVEVDDSFSCHAQDLLWFTKVCHLWEELSWMQQCISPAHACSSCTLQTRLKLLQAAAQLQALLGTVDLGQVYFEPIKDKHGNAVLVLLKDMSACAPLEGLRWIPVSKFQLQRRSSSSSSEEPSALETLLSTLHEKLSYHRRSRKCLPPGLYLGYLKLYTSVDQIRVLVSHKHPNVLCHVKIRDNGHVSREEWQWLQSLTSLEDSAKVCEDAQSAPHRLLLDLRTAAKELLAYVNIPNQQAQDFRVYVKEVVELGEGVSFLLLLPPCEEVCSPPGQNHLCSPRSSFFTLPLQIFELVHFESYSPSFISLYCQVSALLALESLMSQQSLREAFSESELFSAKQKHQQVEEYLQHMEELWRDARWMVILQSARYKHQSSGVSLAWIIDFTKETIPEKPASTSSQVDFLPSPSPSPENTRKHTDSHLSDEDNTSDVFLTTDSSEYSSSVTPSTRELDLLPPSPSSFRSPPSSSSQSSPRSSQPDVLQPVSRREHISVLDSDFILPSRQIELLRVTEKRTALCVRTSSLEYPPTTPTHTASSNQMRWTRPSSFDLCFSAPEPRPSPVRTLSEDSGVQRLSDPAAGSPHRQCHSTLRVYPQYRTGLPKDTSVKLRVTPETCAAELVRLVVQEMNAVCRHLQKATHQCSCAADQCVPSDVCVYSGEQLQHFGLVLLVEGREKWLQDDFCPLTLQNPWTRGKLCVRFKEYSPLALQYSRATTV

Target ORF information:

RefSeq Version	XM_017353975.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin repeat and fibronectin type-III domain-containing protein 1-like (LOC100535772), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353975.1

ORF Insert Sequence:

ATGTTCGCGG GCTCTTATGC TGATGTATTC AGTACGGACA CACTGATGGA GTCTCTAGAG 
AGACGCCTGC AGAGGCCTCT GTTGGCCAAA TCTAGAACAT TACCCAGCAT CCCTCAGTCC 
CCTGCTGCAG TCCGTGTGCA GCAGACGGAG AGTGATTGTG AGCGCCAACA ACGGCCTCGT 
GTTCCTTCTA GCCCTGGGGG TCTTGAAGCC TGCACCATTC CACCTGCAGG TGAGATGTGG 
AGGCTGGAGG ATGAGAGCGA GGCAGACATG GCTCCAGGTG CCGTTGACCT GCGTCCTCGC 
TCTCAGTCAC CGTTCTCACA TTTCAGACGG CGAGCGGCGT ATCTGAGGAA GAGCATCTCA 
GCGGACGATC ATCTAGTGGA TTCAGAGTTC CAGAGCATCC AGACAGAGGA CAGGAGCTCA 
GCTGATCCCA AAGCTAAACT CAAGAGGAAG TTTAGTCTGG GCTCCATCGA CAGGAAAGGA 
ACGCGACAGA GAAGGACTGA ATCTGGGATT GTGAAACTGA CCCAGCGACT CTCTCTGAAG 
GACAAACACT GCAGTAAAGA GGACAGGAGT CTGCAGTCCA GACCTCCGGC CTACAGGAGG 
CGCTCTCTCA GCGTGGACTG GGCCGAATCC AAAATGACTC AACAGATTCC AGACCCTCAT 
CCTCTGATTG CACGGAAATA CAGCACATCA TGTTCGTCTC CACCGAGCGC AGCGCGGCGT 
CTCTACAGGA ACCTGTCGGG GAAATTCAGA ACAGCAAACC TGGTTTTGGA TGATACGACA 
CTCCCGGGAC AGTCGGACAA AGAGCAGTCA CACAAAACCA CCATGTTCCA GGGTAACGAG 
GCTCTGTTTG AAGCGGTGGA GCATCAGGAG CTGGACGTGG TGGAGTCTCT GCTGAACACC 
TTCAGTCTGG AGGAGCTGGA CTTGAACACA CCCAACAGTG AAGGCCTGCT GCCGCTCGAC 
ATCGCCATCA TGACCAACAA CGTGCCCATG GCCAAACTGC TGCTGAGAGC CGGGGCCAAA 
GAGAGTCCCC ACTTTTTAAG TCTTGAAGGT CGAGCTGCTC ACCTGGCTTC TCTGGTGCAA 
GAGGCAGAGC ATCGTATAGC AGAGCTGGTT GCCCAGGTGA CAGGGGAGGA GCCTGGAGAG 
AGGGAGGAGT CAAACAGAGA GAGACAGCTG AAATCATGGG AATGGAAGCT CCGCCTCTAT 
AAGCGCATGC AGACGGGATA CACACATGCC AGTCCTCCCG AACCTCCCAG TGACGTACAT 
CTGTGTGTCA GCAGTGGCAC CAGTCTCAGA GTTTACTTCC AAGAGCCTCT ATGTCACAAT 
TCGGCTGTTA TAACCAAATA CCGCGTGAGC TGGAGCAGTG TTTCTTCATT TAGCCCTTTG 
CTGGGTGAGA CGGTCATTGA AGACGTGACT CAACTCCAGT ATGACATCAC AGGACTCGCT 
GCGGGCACAG GCTGTTATGT CCAGGTCTCA GCATATAATA TGAAGGGATG GAGTTCACCT 
CAGATATCAG AACCTGCTTG TGCTACACCC TCCAGTTGGA GGGAGGTGGA TGGTCTTCCC 
CCCCGCCAGC AGGGTCTGAA AGAGGCGTTA GAGCAGCTCC TGGGGCAAAT TAAAGGGGCG 
CACCGTCACT GCGCATGCCA CGAACAAATC AAAGCTCCGT CGAACACCAG AAAACATTCT 
GTCTCTAAAA GTCTGCGGCA TCTCTTCCAG CCCACCAGCA AATTTGTCAA AAACCTGAAA 
AGAGGTCTTT ATCTAGCCTG TATATTTTAT CAAGATGAAA ACGTTCTGGT GACTCCAGAG 
GATCAGCTGC CGGTTGTAGA GGTGGACGAC TCCTTCAGCT GTCACGCACA GGATTTACTC 
TGGTTCACAA AGGTGTGTCA CCTGTGGGAG GAGTTGAGCT GGATGCAGCA GTGTATCAGT 
CCTGCTCACG CCTGCAGCTC CTGCACACTT CAGACACGCC TCAAACTGCT GCAGGCAGCG 
GCACAACTAC AGGCTCTGCT GGGCACGGTG GATCTGGGAC AGGTGTATTT CGAGCCCATT 
AAAGACAAGC ACGGTAATGC AGTGCTGGTG CTGCTGAAGG ACATGAGTGC GTGTGCGCCG 
CTGGAGGGAC TGCGCTGGAT TCCTGTCAGT AAATTCCAGC TTCAGCGAAG GTCTTCCTCG 
TCTTCATCAG AAGAGCCCAG CGCACTGGAG ACGCTCCTCT CCACACTGCA CGAGAAGCTG 
TCGTATCACC GGCGCAGCAG GAAGTGTCTG CCTCCAGGTC TGTATCTGGG TTACTTGAAG 
CTCTACACCT CAGTGGACCA GATCCGAGTC CTGGTGTCAC ACAAACACCC AAACGTGCTC 
TGCCACGTCA AGATACGAGA CAACGGCCAC GTCTCCAGGG AGGAATGGCA GTGGCTTCAG 
TCACTGACTT CACTGGAGGA CAGCGCGAAG GTGTGTGAGG ATGCTCAGAG TGCTCCGCAT 
CGCCTCCTGC TGGACTTACG AACCGCCGCC AAAGAGTTAC TCGCATACGT CAACATTCCC 
AACCAGCAGG CGCAGGACTT CCGTGTGTAT GTGAAGGAGG TGGTGGAGCT GGGTGAAGGA 
GTCTCATTCC TGCTACTGCT GCCGCCCTGT GAGGAGGTGT GTTCACCACC GGGACAAAAC 
CACCTGTGCT CACCGCGCTC CAGCTTCTTC ACTCTACCAC TGCAGATCTT TGAACTCGTT 
CATTTTGAGT CATACTCGCC CAGTTTCATC TCACTGTACT GTCAAGTGTC GGCTCTGCTG 
GCTCTGGAGT CTCTGATGTC TCAGCAGTCT CTGCGCGAGG CTTTCTCCGA GTCCGAGCTG 
TTCTCCGCCA AGCAGAAACA CCAGCAGGTG GAGGAGTATC TGCAGCATAT GGAGGAGCTG 
TGGCGTGATG CGCGGTGGAT GGTTATTCTC CAGTCTGCCC GATACAAACA TCAGAGTTCT 
GGAGTGAGTT TGGCCTGGAT CATAGACTTC ACTAAGGAAA CCATCCCAGA GAAACCAGCC 
TCCACTTCCT CTCAGGTGGA CTTCCTGCCC TCGCCGAGCC CTTCGCCGGA GAACACACGC 
AAACACACCG ATTCTCATTT GTCGGATGAA GACAACACCT CTGATGTGTT TCTGACCACC 
GACAGCAGCG AGTACAGCTC CAGCGTGACC CCGAGCACCA GAGAGCTGGA TCTTCTTCCT 
CCGTCTCCCT CCAGCTTCAG ATCTCCTCCA TCATCCTCGT CTCAGTCCTC CCCGCGGTCT 
TCTCAGCCAG ACGTCCTGCA GCCAGTGAGC CGCCGTGAGC ACATCAGTGT GTTGGACAGC 
GACTTCATCC TGCCGAGTCG TCAGATCGAG CTCTTACGTG TGACAGAGAA GAGGACAGCT 
CTCTGCGTTC GCACCAGCAG CCTGGAGTAC CCGCCGACCA CGCCCACACA CACCGCCTCC 
TCTAATCAGA TGCGCTGGAC ACGCCCCTCC TCTTTTGATC TGTGTTTCTC TGCCCCCGAG 
CCCCGCCCCT CACCAGTGCG CACTCTATCA GAGGACAGCG GCGTGCAGAG ACTCTCCGAC 
CCCGCGGCCG GGTCTCCGCA CAGACAGTGT CACAGCACAC TGAGAGTTTA CCCACAATAC 
CGCACCGGTT TACCCAAAGA CACCAGCGTC AAATTGCGTG TGACTCCAGA GACGTGTGCT 
GCAGAGCTGG TCCGTCTGGT GGTTCAGGAG ATGAACGCAG TGTGTCGTCA CCTCCAGAAG 
GCCACTCATC AGTGCTCGTG TGCAGCCGAT CAGTGTGTGC CCTCTGACGT GTGTGTGTAC 
AGCGGCGAGC AGCTCCAGCA TTTCGGCCTG GTTCTGCTGG TGGAGGGACG AGAAAAGTGG 
CTGCAGGACG ATTTCTGCCC GCTTACACTG CAAAACCCCT GGACACGCGG GAAACTCTGC 
GTCCGCTTCA AGGAGTATTC ACCACTCGCA CTGCAGTACA GCAGAGCCAC CACTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100535772 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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