frmd4bb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	frmd4bb
Entrez Gene ID	100329900
Full Name	FERM domain containing 4Bb
Synonyms	si:ch73-205c16.1
General protein information	Preferred Names FERM domain containing 4Bb Names FERM domain-containing protein 4B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469864.1	XP_021325539.1	FERM domain-containing protein 4B isoform X4
XM_009296562.3	XP_009294837.1	FERM domain-containing protein 4B isoform X2
XM_009296562.2	XP_009294837.1	FERM domain-containing protein 4B isoform X2
XM_009296561.3	XP_009294836.1	FERM domain-containing protein 4B isoform X1
XM_009296563.2	XP_009294838.1	FERM domain-containing protein 4B isoform X3
XM_009296561.2	XP_009294836.1	FERM domain-containing protein 4B isoform X1
XM_009296563.3	XP_009294838.1	FERM domain-containing protein 4B isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	FRMD4B	NP_055938.1
Xenopus tropicalis (tropical clawed frog)	frmd4b	XP_002940561.2
Canis lupus familiaris (dog)	FRMD4B	XP_005632324.1
Danio rerio (zebrafish)	frmd4bb	XP_002666440.2
Pan troglodytes (chimpanzee)	FRMD4B	XP_516575.3
Rattus norvegicus (Norway rat)	Frmd4b	XP_232212.7
Gallus gallus (chicken)	FRMD4B	XP_423748.2
	FRMD4B	XP_001086554.1
Danio rerio (zebrafish)	LOC100333566	XP_002662555.3
Mus musculus (house mouse)	Frmd4b	NP_660130.2

Gene Ontology

The following frmd4bb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frmd4bb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa27315	XM_009296563.3 Latest version!	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa151503	XM_021469864.1 Latest version!	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa49256	XM_009296561.3 Latest version!	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$629.30 $899.00
ODa49257	XM_009296562.3 Latest version!	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$629.30 $899.00
View Old Accession Versions >>
ODa49257	XM_009296562.2	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$629.30 $899.00
ODa49256	XM_009296561.2	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$629.30 $899.00
ODa27315	XM_009296563.2	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

frmd4bb ( XM_009296563.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294838.1 Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa27315
Clone ID Related Accession (Same CDS sequence)	XM_009296563.2 , XM_009296563.3
Accession Version	XM_009296563.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	FERM domain-containing protein 4B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGG GACGCCTGTG CCAGGTGCAG CTGCTGGACG ACAGAAAGCT GGAGATGTTG 
GTTCAGCCCA AGCTGTTGTC TCGTGAACTC CTGGATTTGG TCTCATCACA TTTCAACCTC 
AAAGAGAAGG AATACTTTGG CTTTACCTAT GTGGACGACA CTGGTCAGTG TAAGTGGTTA 
CAGCTGGAGC GCAGAGTTCT GGAGCACGAC TTCAGCAAGA AATCAGGCCC CATCGCCCTT 
CAGTTCCTGG TCAGGTTTTA CATAGAGAGC ATTTCACTTC TGAAGGAGCA CACGACCGTG 
GAGCTGTTCT TTCTGAACGC CAAGTCAGCC ATCTATAATG GTGACATCGA GGTGGAGAGT 
GAGCTGGTTT TTAAATTAGC AGCTCATGCA TTGCAGGAAT CTAAAGGAGA CTATACAAGT 
GATGAAAACA CCAGAGCAGA CCTGAAGAAA TTTCCAGTAC TGCCCACTAA AGTGCTGAAG 
GAGCATCCGT CACTGGCATA CTGTGAGGAG CGCGTGATGG AGCAGTACAG GCTGTTGAAG 
GGGATCTCCA GAGGACAGGC CATAGTGCAG TATATGACAC TGGTGCAGTC ACTGCCCACA 
TACGGAGTGC ATTATTATGA AGTCAAGGAT AAGCAGGGTA TGCCGTGGTG GCTGGGCATC 
AGTTATAAAG GCATTGGACA GTACGACCAC CAGGATAAAG TAAAACCCCG AAAGCTCTTC 
CAGTGGAAAC AGCTGGAAAA CCTGTATTTC AGAGAGAAGA AGTTTGCTGT GGAAGTCAAT 
GACCCTCACA GGCGAGCAGT GACTAAGCGC ACGTTTGGTC AGACGGGACT GGTGATCCAC 
ACTTGGTACG CGACTCACTC CCTGATCAAA ACCATCTGGG TCATGGCCAT CAGTCAGCAC 
CAGTTCTACC TCAACAGAAA ACAGAGCAAA GCAAAAATCA CCACAGCCAG AAGTCTAGGA 
GACATCGCAG TGGATCTGAC GGAGAACGGA GCATCGAAGA TGAGCAAACT GAGCGCTGGA 
GTGAAGAACC AGCTCATCAT GGCCAGCAAC GGCAGCCTGG TTTCCACAGG TTCGGCTGAT 
TCAGAAATGA ACGACGAGCA GAAAAAAGAG AAGCTTGCGG AGCTGAGAAA GAAGGAGCGA 
GACATTCAGG ACATACTAAG CCAAAAACTG ACCGAGCTGA AGGAAATATG CCTGCGGGAA 
GCCGAACTCA CTGGCAAACT CCCGAAAGAG TATCCGCTTA TTAAAGGAGA AACCCCTCCT 
CATGTGCGCA GGAGAGTCGG GACGGCCTTT AAACTGGACG ACCTTTTCCC ATATAATGAG 
GACCCGTATC TCAGAAATCT GGAGAGCCGC TTTGCATTGC AGCAGAAGAT CGTGGAGGCG 
GCCAAGAAGC TCGCGGCGGA GCCTGACATC TCCAAATTAG TGCGGAAAAA ACGACGGAGG 
AACTGTCTGG ACGCCATGCA GAAACTGCAG GAGATCGAAG ATGAAATGAA CCAATACAGG 
CTGAAGAAAG GACAGAAACC CACGCAGAGA GCCTCTGTGA TCATCGCCGA TGAACTGGCT 
CAGTCCGAGT GCAGCTCTCT GTGTGATAGT CTTCCTCTCG ATGACGATGA TGGCGGTCAG 
AGGCCGCGTT CTCGATCAGT GCAGTGTTCT CCATGTCTCA GTCCTCCTCT GTCTTCATCC 
GGGCTGGATT TCGACCCCGA GAGACGATCT TCATCCAGCG ACACACACCA CGACAGACCT 
CTGAACAGAT TAGATCATGA GGTGCAAGAG CCTGGACATT ATTATACCCC TCGAGAGGTT 
TTCTCCGCCC AGAGCAGTCC CTGTAAAACT TTATCCAGAA TGCCAAAGGA TCCCCGCAGT 
TTACCACAGA CCCCAGTCCT GCCCCGTAAT GCCTACAGCA GCAGTCAACT CCGGTCAGAC 
GGTCACATCC GAGCTTTCCG GCATCGCAGT GGCAGTTTGG AGTCTCAGTC ACAACTTTTC 
CATTCTCACT GTGCAAACAA GTCTGTCTTC CCGCTGTCTC CCTCCCGCCG CAGCAACAGC 
ACTGAGGTGT TGGACGACTG CTCCTCCTAT ACCAGCATGT CCAGCATGGA CTTCTGCCTC 
CCCTGCTCGG CTACTCCTCC TTACCGCACC CTGGACTCAC ACTCCCACGA CTACCGGCTC 
CACCGCAAAG TGGAGATCTA TGGCAATACA GGCAGCATGC CCAACCTGGT CCCTCAAAAC 
TCCAGTTCCT ACCAGCAGAA CCACTACGGA CCAACTGCTT ACTACGTCGC TGGGTATGCT 
GGCTTCGATT ATGAGCCTTG TTCCAATGGA GCTTATGTTT ACGAGAGCGA AATAGAGGGT 
CACTATAACA TCAATCCCAC TTACCCTGCA CATGGCTACC AGGGCCGGGG TCGCAACAGG 
CATTATGGAA TAGAAGGTTT GGATAATTTG TCACAGAATC CATATGCCAC CGTTAGGCCC 
CCTAGAGGAA GGCAGGGACC CTTGAATGAG GTGCTGACGA AGAACATGTA CAGGGCTTTG 
GTGGCAGAGC ATTTAAAGGG ATGGTACAAC CGCAGTGCTG GGCATAGGGA CCCAGGCACC 
TATCAGCTGG TGTACACCTA CAAGTATGAC CGCGGTTCCC AGCACAGCTT GGGCTACCAG 
ACGCTGCCCG CACCCTTCAG CCACTCCAGC CGGACCAACT CCTTCTCCTC AGTGTCGTCG 
ACTGCTAGCG GAGGGGGAAA CTGGCAGAAT CAGTTAGCAG TGGGTCTGGC GGATGGAGAA 
CTGCCGGACG GTCCCTTGAG CGCTGGGGCT TATAGTCCTG CATACAGCCG CAGTCCAACA 
CACAGCAGCC GGTTCCCATC AGAAGGAGCG GCGTCTCACA CTTCAGAGCC ACTGGAGGTG 
TTTGGAGCCG TGGCCAGCTG CTCCGACACA GAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294838.1
CDS	152..3070
Translation	MTEGRLCQVQLLDDRKLEMLVQPKLLSRELLDLVSSHFNLKEKEYFGFTYVDDTGQCKWLQLERRVLEHDFSKKSGPIALQFLVRFYIESISLLKEHTTVELFFLNAKSAIYNGDIEVESELVFKLAAHALQESKGDYTSDENTRADLKKFPVLPTKVLKEHPSLAYCEERVMEQYRLLKGISRGQAIVQYMTLVQSLPTYGVHYYEVKDKQGMPWWLGISYKGIGQYDHQDKVKPRKLFQWKQLENLYFREKKFAVEVNDPHRRAVTKRTFGQTGLVIHTWYATHSLIKTIWVMAISQHQFYLNRKQSKAKITTARSLGDIAVDLTENGASKMSKLSAGVKNQLIMASNGSLVSTGSADSEMNDEQKKEKLAELRKKERDIQDILSQKLTELKEICLREAELTGKLPKEYPLIKGETPPHVRRRVGTAFKLDDLFPYNEDPYLRNLESRFALQQKIVEAAKKLAAEPDISKLVRKKRRRNCLDAMQKLQEIEDEMNQYRLKKGQKPTQRASVIIADELAQSECSSLCDSLPLDDDDGGQRPRSRSVQCSPCLSPPLSSSGLDFDPERRSSSSDTHHDRPLNRLDHEVQEPGHYYTPREVFSAQSSPCKTLSRMPKDPRSLPQTPVLPRNAYSSSQLRSDGHIRAFRHRSGSLESQSQLFHSHCANKSVFPLSPSRRSNSTEVLDDCSSYTSMSSMDFCLPCSATPPYRTLDSHSHDYRLHRKVEIYGNTGSMPNLVPQNSSSYQQNHYGPTAYYVAGYAGFDYEPCSNGAYVYESEIEGHYNINPTYPAHGYQGRGRNRHYGIEGLDNLSQNPYATVRPPRGRQGPLNEVLTKNMYRALVAEHLKGWYNRSAGHRDPGTYQLVYTYKYDRGSQHSLGYQTLPAPFSHSSRTNSFSSVSSTASGGGNWQNQLAVGLADGELPDGPLSAGAYSPAYSRSPTHSSRFPSEGAASHTSEPLEVFGAVASCSDTEN

Target ORF information:

RefSeq Version	XM_009296563.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296563.2

ORF Insert Sequence:

ATGACAGAGG GACGCCTGTG CCAGGTGCAG CTGCTGGACG ACAGAAAGCT GGAGATGTTG 
GTTCAGCCCA AGCTGTTGTC TCGTGAACTC CTGGATTTGG TCTCATCACA TTTCAACCTC 
AAAGAGAAGG AATACTTTGG CTTTACCTAT GTGGACGACA CTGGTCAGTG TAAGTGGTTA 
CAGCTGGAGC GCAGAGTTCT GGAGCACGAC TTCAGCAAGA AATCAGGCCC CATCGCCCTT 
CAGTTCCTGG TCAGGTTTTA CATAGAGAGC ATTTCACTTC TGAAGGAGCA CACGACCGTG 
GAGCTGTTCT TTCTGAACGC CAAGTCAGCC ATCTATAATG GTGACATCGA GGTGGAGAGT 
GAGCTGGTTT TTAAATTAGC AGCTCATGCA TTGCAGGAAT CTAAAGGAGA CTATACAAGT 
GATGAAAACA CCAGAGCAGA CCTGAAGAAA TTTCCAGTAC TGCCCACTAA AGTGCTGAAG 
GAGCATCCGT CACTGGCATA CTGTGAGGAG CGCGTGATGG AGCAGTACAG GCTGTTGAAG 
GGGATCTCCA GAGGACAGGC CATAGTGCAG TATATGACAC TGGTGCAGTC ACTGCCCACA 
TACGGAGTGC ATTATTATGA AGTCAAGGAT AAGCAGGGTA TGCCGTGGTG GCTGGGCATC 
AGTTATAAAG GCATTGGACA GTACGACCAC CAGGATAAAG TAAAACCCCG AAAGCTCTTC 
CAGTGGAAAC AGCTGGAAAA CCTGTATTTC AGAGAGAAGA AGTTTGCTGT GGAAGTCAAT 
GACCCTCACA GGCGAGCAGT GACTAAGCGC ACGTTTGGTC AGACGGGACT GGTGATCCAC 
ACTTGGTACG CGACTCACTC CCTGATCAAA ACCATCTGGG TCATGGCCAT CAGTCAGCAC 
CAGTTCTACC TCAACAGAAA ACAGAGCAAA GCAAAAATCA CCACAGCCAG AAGTCTAGGA 
GACATCGCAG TGGATCTGAC GGAGAACGGA GCATCGAAGA TGAGCAAACT GAGCGCTGGA 
GTGAAGAACC AGCTCATCAT GGCCAGCAAC GGCAGCCTGG TTTCCACAGG TTCGGCTGAT 
TCAGAAATGA ACGACGAGCA GAAAAAAGAG AAGCTTGCGG AGCTGAGAAA GAAGGAGCGA 
GACATTCAGG ACATACTAAG CCAAAAACTG ACCGAGCTGA AGGAAATATG CCTGCGGGAA 
GCCGAACTCA CTGGCAAACT CCCGAAAGAG TATCCGCTTA TTAAAGGAGA AACCCCTCCT 
CATGTGCGCA GGAGAGTCGG GACGGCCTTT AAACTGGACG ACCTTTTCCC ATATAATGAG 
GACCCGTATC TCAGAAATCT GGAGAGCCGC TTTGCATTGC AGCAGAAGAT CGTGGAGGCG 
GCCAAGAAGC TCGCGGCGGA GCCTGACATC TCCAAATTAG TGCGGAAAAA ACGACGGAGG 
AACTGTCTGG ACGCCATGCA GAAACTGCAG GAGATCGAAG ATGAAATGAA CCAATACAGG 
CTGAAGAAAG GACAGAAACC CACGCAGAGA GCCTCTGTGA TCATCGCCGA TGAACTGGCT 
CAGTCCGAGT GCAGCTCTCT GTGTGATAGT CTTCCTCTCG ATGACGATGA TGGCGGTCAG 
AGGCCGCGTT CTCGATCAGT GCAGTGTTCT CCATGTCTCA GTCCTCCTCT GTCTTCATCC 
GGGCTGGATT TCGACCCCGA GAGACGATCT TCATCCAGCG ACACACACCA CGACAGACCT 
CTGAACAGAT TAGATCATGA GGTGCAAGAG CCTGGACATT ATTATACCCC TCGAGAGGTT 
TTCTCCGCCC AGAGCAGTCC CTGTAAAACT TTATCCAGAA TGCCAAAGGA TCCCCGCAGT 
TTACCACAGA CCCCAGTCCT GCCCCGTAAT GCCTACAGCA GCAGTCAACT CCGGTCAGAC 
GGTCACATCC GAGCTTTCCG GCATCGCAGT GGCAGTTTGG AGTCTCAGTC ACAACTTTTC 
CATTCTCACT GTGCAAACAA GTCTGTCTTC CCGCTGTCTC CCTCCCGCCG CAGCAACAGC 
ACTGAGGTGT TGGACGACTG CTCCTCCTAT ACCAGCATGT CCAGCATGGA CTTCTGCCTC 
CCCTGCTCGG CTACTCCTCC TTACCGCACC CTGGACTCAC ACTCCCACGA CTACCGGCTC 
CACCGCAAAG TGGAGATCTA TGGCAATACA GGCAGCATGC CCAACCTGGT CCCTCAAAAC 
TCCAGTTCCT ACCAGCAGAA CCACTACGGA CCAACTGCTT ACTACGTCGC TGGGTATGCT 
GGCTTCGATT ATGAGCCTTG TTCCAATGGA GCTTATGTTT ACGAGAGCGA AATAGAGGGT 
CACTATAACA TCAATCCCAC TTACCCTGCA CATGGCTACC AGGGCCGGGG TCGCAACAGG 
CATTATGGAA TAGAAGGTTT GGATAATTTG TCACAGAATC CATATGCCAC CGTTAGGCCC 
CCTAGAGGAA GGCAGGGACC CTTGAATGAG GTGCTGACGA AGAACATGTA CAGGGCTTTG 
GTGGCAGAGC ATTTAAAGGG ATGGTACAAC CGCAGTGCTG GGCATAGGGA CCCAGGCACC 
TATCAGCTGG TGTACACCTA CAAGTATGAC CGCGGTTCCC AGCACAGCTT GGGCTACCAG 
ACGCTGCCCG CACCCTTCAG CCACTCCAGC CGGACCAACT CCTTCTCCTC AGTGTCGTCG 
ACTGCTAGCG GAGGGGGAAA CTGGCAGAAT CAGTTAGCAG TGGGTCTGGC GGATGGAGAA 
CTGCCGGACG GTCCCTTGAG CGCTGGGGCT TATAGTCCTG CATACAGCCG CAGTCCAACA 
CACAGCAGCC GGTTCCCATC AGAAGGAGCG GCGTCTCACA CTTCAGAGCC ACTGGAGGTG 
TTTGGAGCCG TGGCCAGCTG CTCCGACACA GAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4bb ( XM_009296563.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294838.1 Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa27315
Clone ID Related Accession (Same CDS sequence)	XM_009296563.2 , XM_009296563.3
Accession Version	XM_009296563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	FERM domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGG GACGCCTGTG CCAGGTGCAG CTGCTGGACG ACAGAAAGCT GGAGATGTTG 
GTTCAGCCCA AGCTGTTGTC TCGTGAACTC CTGGATTTGG TCTCATCACA TTTCAACCTC 
AAAGAGAAGG AATACTTTGG CTTTACCTAT GTGGACGACA CTGGTCAGTG TAAGTGGTTA 
CAGCTGGAGC GCAGAGTTCT GGAGCACGAC TTCAGCAAGA AATCAGGCCC CATCGCCCTT 
CAGTTCCTGG TCAGGTTTTA CATAGAGAGC ATTTCACTTC TGAAGGAGCA CACGACCGTG 
GAGCTGTTCT TTCTGAACGC CAAGTCAGCC ATCTATAATG GTGACATCGA GGTGGAGAGT 
GAGCTGGTTT TTAAATTAGC AGCTCATGCA TTGCAGGAAT CTAAAGGAGA CTATACAAGT 
GATGAAAACA CCAGAGCAGA CCTGAAGAAA TTTCCAGTAC TGCCCACTAA AGTGCTGAAG 
GAGCATCCGT CACTGGCATA CTGTGAGGAG CGCGTGATGG AGCAGTACAG GCTGTTGAAG 
GGGATCTCCA GAGGACAGGC CATAGTGCAG TATATGACAC TGGTGCAGTC ACTGCCCACA 
TACGGAGTGC ATTATTATGA AGTCAAGGAT AAGCAGGGTA TGCCGTGGTG GCTGGGCATC 
AGTTATAAAG GCATTGGACA GTACGACCAC CAGGATAAAG TAAAACCCCG AAAGCTCTTC 
CAGTGGAAAC AGCTGGAAAA CCTGTATTTC AGAGAGAAGA AGTTTGCTGT GGAAGTCAAT 
GACCCTCACA GGCGAGCAGT GACTAAGCGC ACGTTTGGTC AGACGGGACT GGTGATCCAC 
ACTTGGTACG CGACTCACTC CCTGATCAAA ACCATCTGGG TCATGGCCAT CAGTCAGCAC 
CAGTTCTACC TCAACAGAAA ACAGAGCAAA GCAAAAATCA CCACAGCCAG AAGTCTAGGA 
GACATCGCAG TGGATCTGAC GGAGAACGGA GCATCGAAGA TGAGCAAACT GAGCGCTGGA 
GTGAAGAACC AGCTCATCAT GGCCAGCAAC GGCAGCCTGG TTTCCACAGG TTCGGCTGAT 
TCAGAAATGA ACGACGAGCA GAAAAAAGAG AAGCTTGCGG AGCTGAGAAA GAAGGAGCGA 
GACATTCAGG ACATACTAAG CCAAAAACTG ACCGAGCTGA AGGAAATATG CCTGCGGGAA 
GCCGAACTCA CTGGCAAACT CCCGAAAGAG TATCCGCTTA TTAAAGGAGA AACCCCTCCT 
CATGTGCGCA GGAGAGTCGG GACGGCCTTT AAACTGGACG ACCTTTTCCC ATATAATGAG 
GACCCGTATC TCAGAAATCT GGAGAGCCGC TTTGCATTGC AGCAGAAGAT CGTGGAGGCG 
GCCAAGAAGC TCGCGGCGGA GCCTGACATC TCCAAATTAG TGCGGAAAAA ACGACGGAGG 
AACTGTCTGG ACGCCATGCA GAAACTGCAG GAGATCGAAG ATGAAATGAA CCAATACAGG 
CTGAAGAAAG GACAGAAACC CACGCAGAGA GCCTCTGTGA TCATCGCCGA TGAACTGGCT 
CAGTCCGAGT GCAGCTCTCT GTGTGATAGT CTTCCTCTCG ATGACGATGA TGGCGGTCAG 
AGGCCGCGTT CTCGATCAGT GCAGTGTTCT CCATGTCTCA GTCCTCCTCT GTCTTCATCC 
GGGCTGGATT TCGACCCCGA GAGACGATCT TCATCCAGCG ACACACACCA CGACAGACCT 
CTGAACAGAT TAGATCATGA GGTGCAAGAG CCTGGACATT ATTATACCCC TCGAGAGGTT 
TTCTCCGCCC AGAGCAGTCC CTGTAAAACT TTATCCAGAA TGCCAAAGGA TCCCCGCAGT 
TTACCACAGA CCCCAGTCCT GCCCCGTAAT GCCTACAGCA GCAGTCAACT CCGGTCAGAC 
GGTCACATCC GAGCTTTCCG GCATCGCAGT GGCAGTTTGG AGTCTCAGTC ACAACTTTTC 
CATTCTCACT GTGCAAACAA GTCTGTCTTC CCGCTGTCTC CCTCCCGCCG CAGCAACAGC 
ACTGAGGTGT TGGACGACTG CTCCTCCTAT ACCAGCATGT CCAGCATGGA CTTCTGCCTC 
CCCTGCTCGG CTACTCCTCC TTACCGCACC CTGGACTCAC ACTCCCACGA CTACCGGCTC 
CACCGCAAAG TGGAGATCTA TGGCAATACA GGCAGCATGC CCAACCTGGT CCCTCAAAAC 
TCCAGTTCCT ACCAGCAGAA CCACTACGGA CCAACTGCTT ACTACGTCGC TGGGTATGCT 
GGCTTCGATT ATGAGCCTTG TTCCAATGGA GCTTATGTTT ACGAGAGCGA AATAGAGGGT 
CACTATAACA TCAATCCCAC TTACCCTGCA CATGGCTACC AGGGCCGGGG TCGCAACAGG 
CATTATGGAA TAGAAGGTTT GGATAATTTG TCACAGAATC CATATGCCAC CGTTAGGCCC 
CCTAGAGGAA GGCAGGGACC CTTGAATGAG GTGCTGACGA AGAACATGTA CAGGGCTTTG 
GTGGCAGAGC ATTTAAAGGG ATGGTACAAC CGCAGTGCTG GGCATAGGGA CCCAGGCACC 
TATCAGCTGG TGTACACCTA CAAGTATGAC CGCGGTTCCC AGCACAGCTT GGGCTACCAG 
ACGCTGCCCG CACCCTTCAG CCACTCCAGC CGGACCAACT CCTTCTCCTC AGTGTCGTCG 
ACTGCTAGCG GAGGGGGAAA CTGGCAGAAT CAGTTAGCAG TGGGTCTGGC GGATGGAGAA 
CTGCCGGACG GTCCCTTGAG CGCTGGGGCT TATAGTCCTG CATACAGCCG CAGTCCAACA 
CACAGCAGCC GGTTCCCATC AGAAGGAGCG GCGTCTCACA CTTCAGAGCC ACTGGAGGTG 
TTTGGAGCCG TGGCCAGCTG CTCCGACACA GAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294838.1
CDS	167..3085
Translation	MTEGRLCQVQLLDDRKLEMLVQPKLLSRELLDLVSSHFNLKEKEYFGFTYVDDTGQCKWLQLERRVLEHDFSKKSGPIALQFLVRFYIESISLLKEHTTVELFFLNAKSAIYNGDIEVESELVFKLAAHALQESKGDYTSDENTRADLKKFPVLPTKVLKEHPSLAYCEERVMEQYRLLKGISRGQAIVQYMTLVQSLPTYGVHYYEVKDKQGMPWWLGISYKGIGQYDHQDKVKPRKLFQWKQLENLYFREKKFAVEVNDPHRRAVTKRTFGQTGLVIHTWYATHSLIKTIWVMAISQHQFYLNRKQSKAKITTARSLGDIAVDLTENGASKMSKLSAGVKNQLIMASNGSLVSTGSADSEMNDEQKKEKLAELRKKERDIQDILSQKLTELKEICLREAELTGKLPKEYPLIKGETPPHVRRRVGTAFKLDDLFPYNEDPYLRNLESRFALQQKIVEAAKKLAAEPDISKLVRKKRRRNCLDAMQKLQEIEDEMNQYRLKKGQKPTQRASVIIADELAQSECSSLCDSLPLDDDDGGQRPRSRSVQCSPCLSPPLSSSGLDFDPERRSSSSDTHHDRPLNRLDHEVQEPGHYYTPREVFSAQSSPCKTLSRMPKDPRSLPQTPVLPRNAYSSSQLRSDGHIRAFRHRSGSLESQSQLFHSHCANKSVFPLSPSRRSNSTEVLDDCSSYTSMSSMDFCLPCSATPPYRTLDSHSHDYRLHRKVEIYGNTGSMPNLVPQNSSSYQQNHYGPTAYYVAGYAGFDYEPCSNGAYVYESEIEGHYNINPTYPAHGYQGRGRNRHYGIEGLDNLSQNPYATVRPPRGRQGPLNEVLTKNMYRALVAEHLKGWYNRSAGHRDPGTYQLVYTYKYDRGSQHSLGYQTLPAPFSHSSRTNSFSSVSSTASGGGNWQNQLAVGLADGELPDGPLSAGAYSPAYSRSPTHSSRFPSEGAASHTSEPLEVFGAVASCSDTEN

Target ORF information:

RefSeq Version	XM_009296563.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296563.3

ORF Insert Sequence:

ATGACAGAGG GACGCCTGTG CCAGGTGCAG CTGCTGGACG ACAGAAAGCT GGAGATGTTG 
GTTCAGCCCA AGCTGTTGTC TCGTGAACTC CTGGATTTGG TCTCATCACA TTTCAACCTC 
AAAGAGAAGG AATACTTTGG CTTTACCTAT GTGGACGACA CTGGTCAGTG TAAGTGGTTA 
CAGCTGGAGC GCAGAGTTCT GGAGCACGAC TTCAGCAAGA AATCAGGCCC CATCGCCCTT 
CAGTTCCTGG TCAGGTTTTA CATAGAGAGC ATTTCACTTC TGAAGGAGCA CACGACCGTG 
GAGCTGTTCT TTCTGAACGC CAAGTCAGCC ATCTATAATG GTGACATCGA GGTGGAGAGT 
GAGCTGGTTT TTAAATTAGC AGCTCATGCA TTGCAGGAAT CTAAAGGAGA CTATACAAGT 
GATGAAAACA CCAGAGCAGA CCTGAAGAAA TTTCCAGTAC TGCCCACTAA AGTGCTGAAG 
GAGCATCCGT CACTGGCATA CTGTGAGGAG CGCGTGATGG AGCAGTACAG GCTGTTGAAG 
GGGATCTCCA GAGGACAGGC CATAGTGCAG TATATGACAC TGGTGCAGTC ACTGCCCACA 
TACGGAGTGC ATTATTATGA AGTCAAGGAT AAGCAGGGTA TGCCGTGGTG GCTGGGCATC 
AGTTATAAAG GCATTGGACA GTACGACCAC CAGGATAAAG TAAAACCCCG AAAGCTCTTC 
CAGTGGAAAC AGCTGGAAAA CCTGTATTTC AGAGAGAAGA AGTTTGCTGT GGAAGTCAAT 
GACCCTCACA GGCGAGCAGT GACTAAGCGC ACGTTTGGTC AGACGGGACT GGTGATCCAC 
ACTTGGTACG CGACTCACTC CCTGATCAAA ACCATCTGGG TCATGGCCAT CAGTCAGCAC 
CAGTTCTACC TCAACAGAAA ACAGAGCAAA GCAAAAATCA CCACAGCCAG AAGTCTAGGA 
GACATCGCAG TGGATCTGAC GGAGAACGGA GCATCGAAGA TGAGCAAACT GAGCGCTGGA 
GTGAAGAACC AGCTCATCAT GGCCAGCAAC GGCAGCCTGG TTTCCACAGG TTCGGCTGAT 
TCAGAAATGA ACGACGAGCA GAAAAAAGAG AAGCTTGCGG AGCTGAGAAA GAAGGAGCGA 
GACATTCAGG ACATACTAAG CCAAAAACTG ACCGAGCTGA AGGAAATATG CCTGCGGGAA 
GCCGAACTCA CTGGCAAACT CCCGAAAGAG TATCCGCTTA TTAAAGGAGA AACCCCTCCT 
CATGTGCGCA GGAGAGTCGG GACGGCCTTT AAACTGGACG ACCTTTTCCC ATATAATGAG 
GACCCGTATC TCAGAAATCT GGAGAGCCGC TTTGCATTGC AGCAGAAGAT CGTGGAGGCG 
GCCAAGAAGC TCGCGGCGGA GCCTGACATC TCCAAATTAG TGCGGAAAAA ACGACGGAGG 
AACTGTCTGG ACGCCATGCA GAAACTGCAG GAGATCGAAG ATGAAATGAA CCAATACAGG 
CTGAAGAAAG GACAGAAACC CACGCAGAGA GCCTCTGTGA TCATCGCCGA TGAACTGGCT 
CAGTCCGAGT GCAGCTCTCT GTGTGATAGT CTTCCTCTCG ATGACGATGA TGGCGGTCAG 
AGGCCGCGTT CTCGATCAGT GCAGTGTTCT CCATGTCTCA GTCCTCCTCT GTCTTCATCC 
GGGCTGGATT TCGACCCCGA GAGACGATCT TCATCCAGCG ACACACACCA CGACAGACCT 
CTGAACAGAT TAGATCATGA GGTGCAAGAG CCTGGACATT ATTATACCCC TCGAGAGGTT 
TTCTCCGCCC AGAGCAGTCC CTGTAAAACT TTATCCAGAA TGCCAAAGGA TCCCCGCAGT 
TTACCACAGA CCCCAGTCCT GCCCCGTAAT GCCTACAGCA GCAGTCAACT CCGGTCAGAC 
GGTCACATCC GAGCTTTCCG GCATCGCAGT GGCAGTTTGG AGTCTCAGTC ACAACTTTTC 
CATTCTCACT GTGCAAACAA GTCTGTCTTC CCGCTGTCTC CCTCCCGCCG CAGCAACAGC 
ACTGAGGTGT TGGACGACTG CTCCTCCTAT ACCAGCATGT CCAGCATGGA CTTCTGCCTC 
CCCTGCTCGG CTACTCCTCC TTACCGCACC CTGGACTCAC ACTCCCACGA CTACCGGCTC 
CACCGCAAAG TGGAGATCTA TGGCAATACA GGCAGCATGC CCAACCTGGT CCCTCAAAAC 
TCCAGTTCCT ACCAGCAGAA CCACTACGGA CCAACTGCTT ACTACGTCGC TGGGTATGCT 
GGCTTCGATT ATGAGCCTTG TTCCAATGGA GCTTATGTTT ACGAGAGCGA AATAGAGGGT 
CACTATAACA TCAATCCCAC TTACCCTGCA CATGGCTACC AGGGCCGGGG TCGCAACAGG 
CATTATGGAA TAGAAGGTTT GGATAATTTG TCACAGAATC CATATGCCAC CGTTAGGCCC 
CCTAGAGGAA GGCAGGGACC CTTGAATGAG GTGCTGACGA AGAACATGTA CAGGGCTTTG 
GTGGCAGAGC ATTTAAAGGG ATGGTACAAC CGCAGTGCTG GGCATAGGGA CCCAGGCACC 
TATCAGCTGG TGTACACCTA CAAGTATGAC CGCGGTTCCC AGCACAGCTT GGGCTACCAG 
ACGCTGCCCG CACCCTTCAG CCACTCCAGC CGGACCAACT CCTTCTCCTC AGTGTCGTCG 
ACTGCTAGCG GAGGGGGAAA CTGGCAGAAT CAGTTAGCAG TGGGTCTGGC GGATGGAGAA 
CTGCCGGACG GTCCCTTGAG CGCTGGGGCT TATAGTCCTG CATACAGCCG CAGTCCAACA 
CACAGCAGCC GGTTCCCATC AGAAGGAGCG GCGTCTCACA CTTCAGAGCC ACTGGAGGTG 
TTTGGAGCCG TGGCCAGCTG CTCCGACACA GAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4bb ( XM_021469864.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325539.1 Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X4, mRNA.

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CloneID	ODa151503
Clone ID Related Accession (Same CDS sequence)	XM_021469864.1
Accession Version	XM_021469864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	FERM domain-containing protein 4B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATCA GTCAGCACCA GTTCTACCTC AACAGAAAAC AGAGCAAAGC AAAAATCACC 
ACAGCCAGAA GTCTAGGAGA CATCGCAGTG GATCTGACGG AGAACGGAGC ATCGAAGATG 
AGCAAACTGA GCGCTGGAGT GAAGAACCAG CTCATCATGG CCAGCAACGG CAGCCTGGTT 
TCCACAGGTT CGGCTGATTC AGAAATGAAC GACGAGCAGA AAAAAGAGAA GCTTGCGGAG 
CTGAGAAAGA AGGAGCGAGA CATTCAGGAC ATACTAAGCC AAAAACTGAC CGAGCTGAAG 
GAAATATGCC TGCGGGAAGC CGAACTCACT GGCAAACTCC CGAAAGAGTA TCCGCTTATT 
AAAGGAGAAA CCCCTCCTCA TGTGCGCAGG AGAGTCGGGA CGGCCTTTAA ACTGGACGAC 
CTTTTCCCAT ATAATGAGGA CCCGTATCTC AGAAATCTGG AGAGCCGCTT TGCATTGCAG 
CAGAAGATCG TGGAGGCGGC CAAGAAGCTC GCGGCGGAGC CTGACATCTC CAAATTAGTG 
CGGAAAAAAC GACGGAGGAA CTGTCTGGAC GCCATGCAGA AACTGCAGGA GATCGAAGAT 
GAAATGAACC AATACAGGCT GAAGAAAGGA CAGAAACCCA CGCAGAGAGC CTCTGTGATC 
ATCGCCGATG AACTGGCTCA GTCCGAGTGC AGCTCTCTGT GTGATAGTCT TCCTCTCGAT 
GACGATGATG GCGGTCAGAG GCCGCGTTCT CGATCAGTGC AGTGTTCTCC ATGTCTCAGT 
CCTCCTCTGT CTTCATCCGG GCTGGATTTC GACCCCGAGA GACGATCTTC ATCCAGCGAC 
ACACACCACG ACAGACCTCT GAACAGATTA GATCATGAGG TGCAAGAGCC TGGACATTAT 
TATACCCCTC GAGAGGTTTT CTCCGCCCAG AGCAGTCCCT GTAAAACTTT ATCCAGAATG 
CCAAAGGATC CCCGCAGTTT ACCACAGACC CCAGTCCTGC CCCGTAATGC CTACAGCAGC 
AGTCAACTCC GGTCAGACGG TCACATCCGA GCTTTCCGGC ATCGCAGTGG CAGTTTGGAG 
TCTCAGTCAC AACTTTTCCA TTCTCACTGT GCAAACAAGT CTGTCTTCCC GCTGTCTCCC 
TCCCGCCGCA GCAACAGCAC TGAGGTGTTG GACGACTGCT CCTCCTATAC CAGCATGTCC 
AGCATGGACT TCTGCCTCCC CTGCTCGGCT ACTCCTCCTT ACCGCACCCT GGACTCACAC 
TCCCACGACT ACCGGCTCCA CCGCAAAGTG GAGATCTATG GCAATACAGG CAGCATGCCC 
AACCTGGTCC CTCAAAACTC CAGTTCCTAC CAGCAGAACC ACTACGGACC AACTGCTTAC 
TACGTCGCTG GGTATGCTGG CTTCGATTAT GAGCCTTGTT CCAATGGAGC TTATGTTTAC 
GAGAGCGAAA TAGAGGGTCA CTATAACATC AATCCCACTT ACCCTGCACA TGGCTACCAG 
GGCCGGGGTC GCAACAGGCA TTATGGAATA GAAGGTTTGG ATAATTTGTC ACAGAATCCA 
TATGCCACCG TTAGGCCCCC TAGAGGAAGG CAGGGACCCT TGAATGAGGT GCTGACGAAG 
AACATGTACA GGGCTTTGGT GGCAGAGCAT TTAAAGGGAT GGTACAACCG CAGTGCTGGG 
CATAGGGACC CAGGCACCTA TCAGCTGGTG TACACCTACA AGTATGACCG CGGTTCCCAG 
CACAGCTTGG GCTACCAGAC GCTGCCCGCA CCCTTCAGCC ACTCCAGCCG GACCAACTCC 
TTCTCCTCAG TGTCGTCGAC TGCTAGCGGA GGGGGAAACT GGCAGAATCA GTTAGCAGTG 
GGTCTGGCGG ATGGAGAACT GCCGGACGGT CCCTTGAGCG CTGGGGCTTA TAGTCCTGCA 
TACAGCCGCA GTCCAACACA CAGCAGCCGG TTCCCATCAG AAGGAGCGGC GTCTCACACT 
TCAGAGCCAC TGGAGGTGTT TGGAGCCGTG GCCAGCTGCT CCGACACAGA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325539.1
CDS	577..2613
Translation	MAISQHQFYLNRKQSKAKITTARSLGDIAVDLTENGASKMSKLSAGVKNQLIMASNGSLVSTGSADSEMNDEQKKEKLAELRKKERDIQDILSQKLTELKEICLREAELTGKLPKEYPLIKGETPPHVRRRVGTAFKLDDLFPYNEDPYLRNLESRFALQQKIVEAAKKLAAEPDISKLVRKKRRRNCLDAMQKLQEIEDEMNQYRLKKGQKPTQRASVIIADELAQSECSSLCDSLPLDDDDGGQRPRSRSVQCSPCLSPPLSSSGLDFDPERRSSSSDTHHDRPLNRLDHEVQEPGHYYTPREVFSAQSSPCKTLSRMPKDPRSLPQTPVLPRNAYSSSQLRSDGHIRAFRHRSGSLESQSQLFHSHCANKSVFPLSPSRRSNSTEVLDDCSSYTSMSSMDFCLPCSATPPYRTLDSHSHDYRLHRKVEIYGNTGSMPNLVPQNSSSYQQNHYGPTAYYVAGYAGFDYEPCSNGAYVYESEIEGHYNINPTYPAHGYQGRGRNRHYGIEGLDNLSQNPYATVRPPRGRQGPLNEVLTKNMYRALVAEHLKGWYNRSAGHRDPGTYQLVYTYKYDRGSQHSLGYQTLPAPFSHSSRTNSFSSVSSTASGGGNWQNQLAVGLADGELPDGPLSAGAYSPAYSRSPTHSSRFPSEGAASHTSEPLEVFGAVASCSDTEN

Target ORF information:

RefSeq Version	XM_021469864.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469864.1

ORF Insert Sequence:

ATGGCCATCA GTCAGCACCA GTTCTACCTC AACAGAAAAC AGAGCAAAGC AAAAATCACC 
ACAGCCAGAA GTCTAGGAGA CATCGCAGTG GATCTGACGG AGAACGGAGC ATCGAAGATG 
AGCAAACTGA GCGCTGGAGT GAAGAACCAG CTCATCATGG CCAGCAACGG CAGCCTGGTT 
TCCACAGGTT CGGCTGATTC AGAAATGAAC GACGAGCAGA AAAAAGAGAA GCTTGCGGAG 
CTGAGAAAGA AGGAGCGAGA CATTCAGGAC ATACTAAGCC AAAAACTGAC CGAGCTGAAG 
GAAATATGCC TGCGGGAAGC CGAACTCACT GGCAAACTCC CGAAAGAGTA TCCGCTTATT 
AAAGGAGAAA CCCCTCCTCA TGTGCGCAGG AGAGTCGGGA CGGCCTTTAA ACTGGACGAC 
CTTTTCCCAT ATAATGAGGA CCCGTATCTC AGAAATCTGG AGAGCCGCTT TGCATTGCAG 
CAGAAGATCG TGGAGGCGGC CAAGAAGCTC GCGGCGGAGC CTGACATCTC CAAATTAGTG 
CGGAAAAAAC GACGGAGGAA CTGTCTGGAC GCCATGCAGA AACTGCAGGA GATCGAAGAT 
GAAATGAACC AATACAGGCT GAAGAAAGGA CAGAAACCCA CGCAGAGAGC CTCTGTGATC 
ATCGCCGATG AACTGGCTCA GTCCGAGTGC AGCTCTCTGT GTGATAGTCT TCCTCTCGAT 
GACGATGATG GCGGTCAGAG GCCGCGTTCT CGATCAGTGC AGTGTTCTCC ATGTCTCAGT 
CCTCCTCTGT CTTCATCCGG GCTGGATTTC GACCCCGAGA GACGATCTTC ATCCAGCGAC 
ACACACCACG ACAGACCTCT GAACAGATTA GATCATGAGG TGCAAGAGCC TGGACATTAT 
TATACCCCTC GAGAGGTTTT CTCCGCCCAG AGCAGTCCCT GTAAAACTTT ATCCAGAATG 
CCAAAGGATC CCCGCAGTTT ACCACAGACC CCAGTCCTGC CCCGTAATGC CTACAGCAGC 
AGTCAACTCC GGTCAGACGG TCACATCCGA GCTTTCCGGC ATCGCAGTGG CAGTTTGGAG 
TCTCAGTCAC AACTTTTCCA TTCTCACTGT GCAAACAAGT CTGTCTTCCC GCTGTCTCCC 
TCCCGCCGCA GCAACAGCAC TGAGGTGTTG GACGACTGCT CCTCCTATAC CAGCATGTCC 
AGCATGGACT TCTGCCTCCC CTGCTCGGCT ACTCCTCCTT ACCGCACCCT GGACTCACAC 
TCCCACGACT ACCGGCTCCA CCGCAAAGTG GAGATCTATG GCAATACAGG CAGCATGCCC 
AACCTGGTCC CTCAAAACTC CAGTTCCTAC CAGCAGAACC ACTACGGACC AACTGCTTAC 
TACGTCGCTG GGTATGCTGG CTTCGATTAT GAGCCTTGTT CCAATGGAGC TTATGTTTAC 
GAGAGCGAAA TAGAGGGTCA CTATAACATC AATCCCACTT ACCCTGCACA TGGCTACCAG 
GGCCGGGGTC GCAACAGGCA TTATGGAATA GAAGGTTTGG ATAATTTGTC ACAGAATCCA 
TATGCCACCG TTAGGCCCCC TAGAGGAAGG CAGGGACCCT TGAATGAGGT GCTGACGAAG 
AACATGTACA GGGCTTTGGT GGCAGAGCAT TTAAAGGGAT GGTACAACCG CAGTGCTGGG 
CATAGGGACC CAGGCACCTA TCAGCTGGTG TACACCTACA AGTATGACCG CGGTTCCCAG 
CACAGCTTGG GCTACCAGAC GCTGCCCGCA CCCTTCAGCC ACTCCAGCCG GACCAACTCC 
TTCTCCTCAG TGTCGTCGAC TGCTAGCGGA GGGGGAAACT GGCAGAATCA GTTAGCAGTG 
GGTCTGGCGG ATGGAGAACT GCCGGACGGT CCCTTGAGCG CTGGGGCTTA TAGTCCTGCA 
TACAGCCGCA GTCCAACACA CAGCAGCCGG TTCCCATCAG AAGGAGCGGC GTCTCACACT 
TCAGAGCCAC TGGAGGTGTT TGGAGCCGTG GCCAGCTGCT CCGACACAGA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4bb ( XM_009296561.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294836.1 Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49256
Clone ID Related Accession (Same CDS sequence)	XM_009296561.3 , XM_009296561.2
Accession Version	XM_009296561.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	FERM domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGAACTG 
GCTCAGTCCG AGTGCAGCTC TCTGTGTGAT AGTCTTCCTC TCGATGACGA TGATGGCGGT 
CAGAGGCCGC GTTCTCGATC AGTGCAGTGT TCTCCATGTC TCAGTCCTCC TCTGTCTTCA 
TCCGGGCTGG ATTTCGACCC CGAGAGACGA TCTTCATCCA GCGACACACA CCACGACAGA 
CCTCTGAACA GATTAGATCA TGAGGTGCAA GAGCCTGGAC ATTATTATAC CCCTCGAGAG 
GTTTTCTCCG CCCAGAGCAG TCCCTGTAAA ACTTTATCCA GAATGCCAAA GGATCCCCGC 
AGTTTACCAC AGACCCCAGT CCTGCCCCGT AATGCCTACA GCAGCAGTCA ACTCCGGTCA 
GACGGTCACA TCCGAGCTTT CCGGCATCGC AGTGGCAGTT TGGAGTCTCA GTCACAACTT 
TTCCATTCTC ACTGTGCAAA CAAGTCTGTC TTCCCGCTGT CTCCCTCCCG CCGCAGCAAC 
AGCACTGAGG TGTTGGACGA CTGCTCCTCC TATACCAGCA TGTCCAGCAT GGACTTCTGC 
CTCCCCTGCT CGGCTACTCC TCCTTACCGC ACCCTGGACT CACACTCCCA CGACTACCGG 
CTCCACCGCA AAGTGGAGAT CTATGGCAAT ACAGGCAGCA TGCCCAACCT GGTCCCTCAA 
AACTCCAGTT CCTACCAGCA GAACCACTAC GGACCAACTG CTTACTACGT CGCTGGGTAT 
GCTGGCTTCG ATTATGAGCC TTGTTCCAAT GGAGCTTATG TTTACGAGAG CGAAATAGAG 
GGTCACTATA ACATCAATCC CACTTACCCT GCACATGGCT ACCAGGGCCG GGGTCGCAAC 
AGGCATTATG GAATAGAAGG TTTGGATAAT TTGTCACAGA ATCCATATGC CACCGTTAGG 
CCCCCTAGAG GAAGGCAGGG ACCCTTGAAT GAGGTGCTGA CGAAGAACAT GTACAGGGCT 
TTGGTGGCAG AGCATTTAAA GGGATGGTAC AACCGCAGTG CTGGGCATAG GGACCCAGGC 
ACCTATCAGC TGGTGTACAC CTACAAGTAT GACCGCGGTT CCCAGCACAG CTTGGGCTAC 
CAGACGCTGC CCGCACCCTT CAGCCACTCC AGCCGGACCA ACTCCTTCTC CTCAGTGTCG 
TCGACTGCTA GCGGAGGGGG AAACTGGCAG AATCAGTTAG CAGTGGGTCT GGCGGATGGA 
GAACTGCCGG ACGGTCCCTT GAGCGCTGGG GCTTATAGTC CTGCATACAG CCGCAGTCCA 
ACACACAGCA GCCGGTTCCC ATCAGAAGGA GCGGCGTCTC ACACTTCAGA GCCACTGGAG 
GTGTTTGGAG CCGTGGCCAG CTGCTCCGAC ACAGAAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294836.1
CDS	93..3254
Translation	MKEQRSGSAERVICVCVCVCSVTRLCNPGVRAAMVWGLVCVCVCVFRAAGFIWRLVFWAVGRMRCCQESVQTCFYLRHVYQMTEGRLCQVQLLDDRKLEMLVQPKLLSRELLDLVSSHFNLKEKEYFGFTYVDDTGQCKWLQLERRVLEHDFSKKSGPIALQFLVRFYIESISLLKEHTTVELFFLNAKSAIYNGDIEVESELVFKLAAHALQESKGDYTSDENTRADLKKFPVLPTKVLKEHPSLAYCEERVMEQYRLLKGISRGQAIVQYMTLVQSLPTYGVHYYEVKDKQGMPWWLGISYKGIGQYDHQDKVKPRKLFQWKQLENLYFREKKFAVEVNDPHRRAVTKRTFGQTGLVIHTWYATHSLIKTIWVMAISQHQFYLNRKQSKAKITTARSLGDIAVDLTENGASKMSKLSAGVKNQLIMASNGSLVSTGSADSEMNDEQKKEKLAELRKKERDIQDILSQKLTELKEICLREAELTGKLPKEYPLIKGETPPHVRRRVGTAFKLDDLFPYNEDPYLRNLESRFALQQKIVEAAKKLAAEPDISKLVRKKRRRNCLDAMQKLQEIEDEMNQYRLKKGQKPTQRASVIIADELAQSECSSLCDSLPLDDDDGGQRPRSRSVQCSPCLSPPLSSSGLDFDPERRSSSSDTHHDRPLNRLDHEVQEPGHYYTPREVFSAQSSPCKTLSRMPKDPRSLPQTPVLPRNAYSSSQLRSDGHIRAFRHRSGSLESQSQLFHSHCANKSVFPLSPSRRSNSTEVLDDCSSYTSMSSMDFCLPCSATPPYRTLDSHSHDYRLHRKVEIYGNTGSMPNLVPQNSSSYQQNHYGPTAYYVAGYAGFDYEPCSNGAYVYESEIEGHYNINPTYPAHGYQGRGRNRHYGIEGLDNLSQNPYATVRPPRGRQGPLNEVLTKNMYRALVAEHLKGWYNRSAGHRDPGTYQLVYTYKYDRGSQHSLGYQTLPAPFSHSSRTNSFSSVSSTASGGGNWQNQLAVGLADGELPDGPLSAGAYSPAYSRSPTHSSRFPSEGAASHTSEPLEVFGAVASCSDTEN

Target ORF information:

RefSeq Version	XM_009296561.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296561.3

ORF Insert Sequence:

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGAACTG 
GCTCAGTCCG AGTGCAGCTC TCTGTGTGAT AGTCTTCCTC TCGATGACGA TGATGGCGGT 
CAGAGGCCGC GTTCTCGATC AGTGCAGTGT TCTCCATGTC TCAGTCCTCC TCTGTCTTCA 
TCCGGGCTGG ATTTCGACCC CGAGAGACGA TCTTCATCCA GCGACACACA CCACGACAGA 
CCTCTGAACA GATTAGATCA TGAGGTGCAA GAGCCTGGAC ATTATTATAC CCCTCGAGAG 
GTTTTCTCCG CCCAGAGCAG TCCCTGTAAA ACTTTATCCA GAATGCCAAA GGATCCCCGC 
AGTTTACCAC AGACCCCAGT CCTGCCCCGT AATGCCTACA GCAGCAGTCA ACTCCGGTCA 
GACGGTCACA TCCGAGCTTT CCGGCATCGC AGTGGCAGTT TGGAGTCTCA GTCACAACTT 
TTCCATTCTC ACTGTGCAAA CAAGTCTGTC TTCCCGCTGT CTCCCTCCCG CCGCAGCAAC 
AGCACTGAGG TGTTGGACGA CTGCTCCTCC TATACCAGCA TGTCCAGCAT GGACTTCTGC 
CTCCCCTGCT CGGCTACTCC TCCTTACCGC ACCCTGGACT CACACTCCCA CGACTACCGG 
CTCCACCGCA AAGTGGAGAT CTATGGCAAT ACAGGCAGCA TGCCCAACCT GGTCCCTCAA 
AACTCCAGTT CCTACCAGCA GAACCACTAC GGACCAACTG CTTACTACGT CGCTGGGTAT 
GCTGGCTTCG ATTATGAGCC TTGTTCCAAT GGAGCTTATG TTTACGAGAG CGAAATAGAG 
GGTCACTATA ACATCAATCC CACTTACCCT GCACATGGCT ACCAGGGCCG GGGTCGCAAC 
AGGCATTATG GAATAGAAGG TTTGGATAAT TTGTCACAGA ATCCATATGC CACCGTTAGG 
CCCCCTAGAG GAAGGCAGGG ACCCTTGAAT GAGGTGCTGA CGAAGAACAT GTACAGGGCT 
TTGGTGGCAG AGCATTTAAA GGGATGGTAC AACCGCAGTG CTGGGCATAG GGACCCAGGC 
ACCTATCAGC TGGTGTACAC CTACAAGTAT GACCGCGGTT CCCAGCACAG CTTGGGCTAC 
CAGACGCTGC CCGCACCCTT CAGCCACTCC AGCCGGACCA ACTCCTTCTC CTCAGTGTCG 
TCGACTGCTA GCGGAGGGGG AAACTGGCAG AATCAGTTAG CAGTGGGTCT GGCGGATGGA 
GAACTGCCGG ACGGTCCCTT GAGCGCTGGG GCTTATAGTC CTGCATACAG CCGCAGTCCA 
ACACACAGCA GCCGGTTCCC ATCAGAAGGA GCGGCGTCTC ACACTTCAGA GCCACTGGAG 
GTGTTTGGAG CCGTGGCCAG CTGCTCCGAC ACAGAAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4bb ( XM_009296561.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294836.1 Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49256
Clone ID Related Accession (Same CDS sequence)	XM_009296561.3 , XM_009296561.2
Accession Version	XM_009296561.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	FERM domain-containing protein 4B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGAACTG 
GCTCAGTCCG AGTGCAGCTC TCTGTGTGAT AGTCTTCCTC TCGATGACGA TGATGGCGGT 
CAGAGGCCGC GTTCTCGATC AGTGCAGTGT TCTCCATGTC TCAGTCCTCC TCTGTCTTCA 
TCCGGGCTGG ATTTCGACCC CGAGAGACGA TCTTCATCCA GCGACACACA CCACGACAGA 
CCTCTGAACA GATTAGATCA TGAGGTGCAA GAGCCTGGAC ATTATTATAC CCCTCGAGAG 
GTTTTCTCCG CCCAGAGCAG TCCCTGTAAA ACTTTATCCA GAATGCCAAA GGATCCCCGC 
AGTTTACCAC AGACCCCAGT CCTGCCCCGT AATGCCTACA GCAGCAGTCA ACTCCGGTCA 
GACGGTCACA TCCGAGCTTT CCGGCATCGC AGTGGCAGTT TGGAGTCTCA GTCACAACTT 
TTCCATTCTC ACTGTGCAAA CAAGTCTGTC TTCCCGCTGT CTCCCTCCCG CCGCAGCAAC 
AGCACTGAGG TGTTGGACGA CTGCTCCTCC TATACCAGCA TGTCCAGCAT GGACTTCTGC 
CTCCCCTGCT CGGCTACTCC TCCTTACCGC ACCCTGGACT CACACTCCCA CGACTACCGG 
CTCCACCGCA AAGTGGAGAT CTATGGCAAT ACAGGCAGCA TGCCCAACCT GGTCCCTCAA 
AACTCCAGTT CCTACCAGCA GAACCACTAC GGACCAACTG CTTACTACGT CGCTGGGTAT 
GCTGGCTTCG ATTATGAGCC TTGTTCCAAT GGAGCTTATG TTTACGAGAG CGAAATAGAG 
GGTCACTATA ACATCAATCC CACTTACCCT GCACATGGCT ACCAGGGCCG GGGTCGCAAC 
AGGCATTATG GAATAGAAGG TTTGGATAAT TTGTCACAGA ATCCATATGC CACCGTTAGG 
CCCCCTAGAG GAAGGCAGGG ACCCTTGAAT GAGGTGCTGA CGAAGAACAT GTACAGGGCT 
TTGGTGGCAG AGCATTTAAA GGGATGGTAC AACCGCAGTG CTGGGCATAG GGACCCAGGC 
ACCTATCAGC TGGTGTACAC CTACAAGTAT GACCGCGGTT CCCAGCACAG CTTGGGCTAC 
CAGACGCTGC CCGCACCCTT CAGCCACTCC AGCCGGACCA ACTCCTTCTC CTCAGTGTCG 
TCGACTGCTA GCGGAGGGGG AAACTGGCAG AATCAGTTAG CAGTGGGTCT GGCGGATGGA 
GAACTGCCGG ACGGTCCCTT GAGCGCTGGG GCTTATAGTC CTGCATACAG CCGCAGTCCA 
ACACACAGCA GCCGGTTCCC ATCAGAAGGA GCGGCGTCTC ACACTTCAGA GCCACTGGAG 
GTGTTTGGAG CCGTGGCCAG CTGCTCCGAC ACAGAAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294836.1
CDS	94..3255
Translation	MKEQRSGSAERVICVCVCVCSVTRLCNPGVRAAMVWGLVCVCVCVFRAAGFIWRLVFWAVGRMRCCQESVQTCFYLRHVYQMTEGRLCQVQLLDDRKLEMLVQPKLLSRELLDLVSSHFNLKEKEYFGFTYVDDTGQCKWLQLERRVLEHDFSKKSGPIALQFLVRFYIESISLLKEHTTVELFFLNAKSAIYNGDIEVESELVFKLAAHALQESKGDYTSDENTRADLKKFPVLPTKVLKEHPSLAYCEERVMEQYRLLKGISRGQAIVQYMTLVQSLPTYGVHYYEVKDKQGMPWWLGISYKGIGQYDHQDKVKPRKLFQWKQLENLYFREKKFAVEVNDPHRRAVTKRTFGQTGLVIHTWYATHSLIKTIWVMAISQHQFYLNRKQSKAKITTARSLGDIAVDLTENGASKMSKLSAGVKNQLIMASNGSLVSTGSADSEMNDEQKKEKLAELRKKERDIQDILSQKLTELKEICLREAELTGKLPKEYPLIKGETPPHVRRRVGTAFKLDDLFPYNEDPYLRNLESRFALQQKIVEAAKKLAAEPDISKLVRKKRRRNCLDAMQKLQEIEDEMNQYRLKKGQKPTQRASVIIADELAQSECSSLCDSLPLDDDDGGQRPRSRSVQCSPCLSPPLSSSGLDFDPERRSSSSDTHHDRPLNRLDHEVQEPGHYYTPREVFSAQSSPCKTLSRMPKDPRSLPQTPVLPRNAYSSSQLRSDGHIRAFRHRSGSLESQSQLFHSHCANKSVFPLSPSRRSNSTEVLDDCSSYTSMSSMDFCLPCSATPPYRTLDSHSHDYRLHRKVEIYGNTGSMPNLVPQNSSSYQQNHYGPTAYYVAGYAGFDYEPCSNGAYVYESEIEGHYNINPTYPAHGYQGRGRNRHYGIEGLDNLSQNPYATVRPPRGRQGPLNEVLTKNMYRALVAEHLKGWYNRSAGHRDPGTYQLVYTYKYDRGSQHSLGYQTLPAPFSHSSRTNSFSSVSSTASGGGNWQNQLAVGLADGELPDGPLSAGAYSPAYSRSPTHSSRFPSEGAASHTSEPLEVFGAVASCSDTEN

Target ORF information:

RefSeq Version	XM_009296561.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296561.2

ORF Insert Sequence:

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGAACTG 
GCTCAGTCCG AGTGCAGCTC TCTGTGTGAT AGTCTTCCTC TCGATGACGA TGATGGCGGT 
CAGAGGCCGC GTTCTCGATC AGTGCAGTGT TCTCCATGTC TCAGTCCTCC TCTGTCTTCA 
TCCGGGCTGG ATTTCGACCC CGAGAGACGA TCTTCATCCA GCGACACACA CCACGACAGA 
CCTCTGAACA GATTAGATCA TGAGGTGCAA GAGCCTGGAC ATTATTATAC CCCTCGAGAG 
GTTTTCTCCG CCCAGAGCAG TCCCTGTAAA ACTTTATCCA GAATGCCAAA GGATCCCCGC 
AGTTTACCAC AGACCCCAGT CCTGCCCCGT AATGCCTACA GCAGCAGTCA ACTCCGGTCA 
GACGGTCACA TCCGAGCTTT CCGGCATCGC AGTGGCAGTT TGGAGTCTCA GTCACAACTT 
TTCCATTCTC ACTGTGCAAA CAAGTCTGTC TTCCCGCTGT CTCCCTCCCG CCGCAGCAAC 
AGCACTGAGG TGTTGGACGA CTGCTCCTCC TATACCAGCA TGTCCAGCAT GGACTTCTGC 
CTCCCCTGCT CGGCTACTCC TCCTTACCGC ACCCTGGACT CACACTCCCA CGACTACCGG 
CTCCACCGCA AAGTGGAGAT CTATGGCAAT ACAGGCAGCA TGCCCAACCT GGTCCCTCAA 
AACTCCAGTT CCTACCAGCA GAACCACTAC GGACCAACTG CTTACTACGT CGCTGGGTAT 
GCTGGCTTCG ATTATGAGCC TTGTTCCAAT GGAGCTTATG TTTACGAGAG CGAAATAGAG 
GGTCACTATA ACATCAATCC CACTTACCCT GCACATGGCT ACCAGGGCCG GGGTCGCAAC 
AGGCATTATG GAATAGAAGG TTTGGATAAT TTGTCACAGA ATCCATATGC CACCGTTAGG 
CCCCCTAGAG GAAGGCAGGG ACCCTTGAAT GAGGTGCTGA CGAAGAACAT GTACAGGGCT 
TTGGTGGCAG AGCATTTAAA GGGATGGTAC AACCGCAGTG CTGGGCATAG GGACCCAGGC 
ACCTATCAGC TGGTGTACAC CTACAAGTAT GACCGCGGTT CCCAGCACAG CTTGGGCTAC 
CAGACGCTGC CCGCACCCTT CAGCCACTCC AGCCGGACCA ACTCCTTCTC CTCAGTGTCG 
TCGACTGCTA GCGGAGGGGG AAACTGGCAG AATCAGTTAG CAGTGGGTCT GGCGGATGGA 
GAACTGCCGG ACGGTCCCTT GAGCGCTGGG GCTTATAGTC CTGCATACAG CCGCAGTCCA 
ACACACAGCA GCCGGTTCCC ATCAGAAGGA GCGGCGTCTC ACACTTCAGA GCCACTGGAG 
GTGTTTGGAG CCGTGGCCAG CTGCTCCGAC ACAGAAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4bb ( XM_009296562.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294837.1 Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49257
Clone ID Related Accession (Same CDS sequence)	XM_009296562.3 , XM_009296562.2
Accession Version	XM_009296562.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	FERM domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGATGGC 
GGTCAGAGGC CGCGTTCTCG ATCAGTGCAG TGTTCTCCAT GTCTCAGTCC TCCTCTGTCT 
TCATCCGGGC TGGATTTCGA CCCCGAGAGA CGATCTTCAT CCAGCGACAC ACACCACGAC 
AGACCTCTGA ACAGATTAGA TCATGAGGTG CAAGAGCCTG GACATTATTA TACCCCTCGA 
GAGGTTTTCT CCGCCCAGAG CAGTCCCTGT AAAACTTTAT CCAGAATGCC AAAGGATCCC 
CGCAGTTTAC CACAGACCCC AGTCCTGCCC CGTAATGCCT ACAGCAGCAG TCAACTCCGG 
TCAGACGGTC ACATCCGAGC TTTCCGGCAT CGCAGTGGCA GTTTGGAGTC TCAGTCACAA 
CTTTTCCATT CTCACTGTGC AAACAAGTCT GTCTTCCCGC TGTCTCCCTC CCGCCGCAGC 
AACAGCACTG AGGTGTTGGA CGACTGCTCC TCCTATACCA GCATGTCCAG CATGGACTTC 
TGCCTCCCCT GCTCGGCTAC TCCTCCTTAC CGCACCCTGG ACTCACACTC CCACGACTAC 
CGGCTCCACC GCAAAGTGGA GATCTATGGC AATACAGGCA GCATGCCCAA CCTGGTCCCT 
CAAAACTCCA GTTCCTACCA GCAGAACCAC TACGGACCAA CTGCTTACTA CGTCGCTGGG 
TATGCTGGCT TCGATTATGA GCCTTGTTCC AATGGAGCTT ATGTTTACGA GAGCGAAATA 
GAGGGTCACT ATAACATCAA TCCCACTTAC CCTGCACATG GCTACCAGGG CCGGGGTCGC 
AACAGGCATT ATGGAATAGA AGGTTTGGAT AATTTGTCAC AGAATCCATA TGCCACCGTT 
AGGCCCCCTA GAGGAAGGCA GGGACCCTTG AATGAGGTGC TGACGAAGAA CATGTACAGG 
GCTTTGGTGG CAGAGCATTT AAAGGGATGG TACAACCGCA GTGCTGGGCA TAGGGACCCA 
GGCACCTATC AGCTGGTGTA CACCTACAAG TATGACCGCG GTTCCCAGCA CAGCTTGGGC 
TACCAGACGC TGCCCGCACC CTTCAGCCAC TCCAGCCGGA CCAACTCCTT CTCCTCAGTG 
TCGTCGACTG CTAGCGGAGG GGGAAACTGG CAGAATCAGT TAGCAGTGGG TCTGGCGGAT 
GGAGAACTGC CGGACGGTCC CTTGAGCGCT GGGGCTTATA GTCCTGCATA CAGCCGCAGT 
CCAACACACA GCAGCCGGTT CCCATCAGAA GGAGCGGCGT CTCACACTTC AGAGCCACTG 
GAGGTGTTTG GAGCCGTGGC CAGCTGCTCC GACACAGAAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294837.1
CDS	94..3198
Translation	MKEQRSGSAERVICVCVCVCSVTRLCNPGVRAAMVWGLVCVCVCVFRAAGFIWRLVFWAVGRMRCCQESVQTCFYLRHVYQMTEGRLCQVQLLDDRKLEMLVQPKLLSRELLDLVSSHFNLKEKEYFGFTYVDDTGQCKWLQLERRVLEHDFSKKSGPIALQFLVRFYIESISLLKEHTTVELFFLNAKSAIYNGDIEVESELVFKLAAHALQESKGDYTSDENTRADLKKFPVLPTKVLKEHPSLAYCEERVMEQYRLLKGISRGQAIVQYMTLVQSLPTYGVHYYEVKDKQGMPWWLGISYKGIGQYDHQDKVKPRKLFQWKQLENLYFREKKFAVEVNDPHRRAVTKRTFGQTGLVIHTWYATHSLIKTIWVMAISQHQFYLNRKQSKAKITTARSLGDIAVDLTENGASKMSKLSAGVKNQLIMASNGSLVSTGSADSEMNDEQKKEKLAELRKKERDIQDILSQKLTELKEICLREAELTGKLPKEYPLIKGETPPHVRRRVGTAFKLDDLFPYNEDPYLRNLESRFALQQKIVEAAKKLAAEPDISKLVRKKRRRNCLDAMQKLQEIEDEMNQYRLKKGQKPTQRASVIIADDGGQRPRSRSVQCSPCLSPPLSSSGLDFDPERRSSSSDTHHDRPLNRLDHEVQEPGHYYTPREVFSAQSSPCKTLSRMPKDPRSLPQTPVLPRNAYSSSQLRSDGHIRAFRHRSGSLESQSQLFHSHCANKSVFPLSPSRRSNSTEVLDDCSSYTSMSSMDFCLPCSATPPYRTLDSHSHDYRLHRKVEIYGNTGSMPNLVPQNSSSYQQNHYGPTAYYVAGYAGFDYEPCSNGAYVYESEIEGHYNINPTYPAHGYQGRGRNRHYGIEGLDNLSQNPYATVRPPRGRQGPLNEVLTKNMYRALVAEHLKGWYNRSAGHRDPGTYQLVYTYKYDRGSQHSLGYQTLPAPFSHSSRTNSFSSVSSTASGGGNWQNQLAVGLADGELPDGPLSAGAYSPAYSRSPTHSSRFPSEGAASHTSEPLEVFGAVASCSDTEN

Target ORF information:

RefSeq Version	XM_009296562.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296562.3

ORF Insert Sequence:

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGATGGC 
GGTCAGAGGC CGCGTTCTCG ATCAGTGCAG TGTTCTCCAT GTCTCAGTCC TCCTCTGTCT 
TCATCCGGGC TGGATTTCGA CCCCGAGAGA CGATCTTCAT CCAGCGACAC ACACCACGAC 
AGACCTCTGA ACAGATTAGA TCATGAGGTG CAAGAGCCTG GACATTATTA TACCCCTCGA 
GAGGTTTTCT CCGCCCAGAG CAGTCCCTGT AAAACTTTAT CCAGAATGCC AAAGGATCCC 
CGCAGTTTAC CACAGACCCC AGTCCTGCCC CGTAATGCCT ACAGCAGCAG TCAACTCCGG 
TCAGACGGTC ACATCCGAGC TTTCCGGCAT CGCAGTGGCA GTTTGGAGTC TCAGTCACAA 
CTTTTCCATT CTCACTGTGC AAACAAGTCT GTCTTCCCGC TGTCTCCCTC CCGCCGCAGC 
AACAGCACTG AGGTGTTGGA CGACTGCTCC TCCTATACCA GCATGTCCAG CATGGACTTC 
TGCCTCCCCT GCTCGGCTAC TCCTCCTTAC CGCACCCTGG ACTCACACTC CCACGACTAC 
CGGCTCCACC GCAAAGTGGA GATCTATGGC AATACAGGCA GCATGCCCAA CCTGGTCCCT 
CAAAACTCCA GTTCCTACCA GCAGAACCAC TACGGACCAA CTGCTTACTA CGTCGCTGGG 
TATGCTGGCT TCGATTATGA GCCTTGTTCC AATGGAGCTT ATGTTTACGA GAGCGAAATA 
GAGGGTCACT ATAACATCAA TCCCACTTAC CCTGCACATG GCTACCAGGG CCGGGGTCGC 
AACAGGCATT ATGGAATAGA AGGTTTGGAT AATTTGTCAC AGAATCCATA TGCCACCGTT 
AGGCCCCCTA GAGGAAGGCA GGGACCCTTG AATGAGGTGC TGACGAAGAA CATGTACAGG 
GCTTTGGTGG CAGAGCATTT AAAGGGATGG TACAACCGCA GTGCTGGGCA TAGGGACCCA 
GGCACCTATC AGCTGGTGTA CACCTACAAG TATGACCGCG GTTCCCAGCA CAGCTTGGGC 
TACCAGACGC TGCCCGCACC CTTCAGCCAC TCCAGCCGGA CCAACTCCTT CTCCTCAGTG 
TCGTCGACTG CTAGCGGAGG GGGAAACTGG CAGAATCAGT TAGCAGTGGG TCTGGCGGAT 
GGAGAACTGC CGGACGGTCC CTTGAGCGCT GGGGCTTATA GTCCTGCATA CAGCCGCAGT 
CCAACACACA GCAGCCGGTT CCCATCAGAA GGAGCGGCGT CTCACACTTC AGAGCCACTG 
GAGGTGTTTG GAGCCGTGGC CAGCTGCTCC GACACAGAAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4bb ( XM_009296562.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294837.1 Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X2, mRNA.

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CloneID	ODa49257
Clone ID Related Accession (Same CDS sequence)	XM_009296562.3 , XM_009296562.2
Accession Version	XM_009296562.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	FERM domain-containing protein 4B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGATGGC 
GGTCAGAGGC CGCGTTCTCG ATCAGTGCAG TGTTCTCCAT GTCTCAGTCC TCCTCTGTCT 
TCATCCGGGC TGGATTTCGA CCCCGAGAGA CGATCTTCAT CCAGCGACAC ACACCACGAC 
AGACCTCTGA ACAGATTAGA TCATGAGGTG CAAGAGCCTG GACATTATTA TACCCCTCGA 
GAGGTTTTCT CCGCCCAGAG CAGTCCCTGT AAAACTTTAT CCAGAATGCC AAAGGATCCC 
CGCAGTTTAC CACAGACCCC AGTCCTGCCC CGTAATGCCT ACAGCAGCAG TCAACTCCGG 
TCAGACGGTC ACATCCGAGC TTTCCGGCAT CGCAGTGGCA GTTTGGAGTC TCAGTCACAA 
CTTTTCCATT CTCACTGTGC AAACAAGTCT GTCTTCCCGC TGTCTCCCTC CCGCCGCAGC 
AACAGCACTG AGGTGTTGGA CGACTGCTCC TCCTATACCA GCATGTCCAG CATGGACTTC 
TGCCTCCCCT GCTCGGCTAC TCCTCCTTAC CGCACCCTGG ACTCACACTC CCACGACTAC 
CGGCTCCACC GCAAAGTGGA GATCTATGGC AATACAGGCA GCATGCCCAA CCTGGTCCCT 
CAAAACTCCA GTTCCTACCA GCAGAACCAC TACGGACCAA CTGCTTACTA CGTCGCTGGG 
TATGCTGGCT TCGATTATGA GCCTTGTTCC AATGGAGCTT ATGTTTACGA GAGCGAAATA 
GAGGGTCACT ATAACATCAA TCCCACTTAC CCTGCACATG GCTACCAGGG CCGGGGTCGC 
AACAGGCATT ATGGAATAGA AGGTTTGGAT AATTTGTCAC AGAATCCATA TGCCACCGTT 
AGGCCCCCTA GAGGAAGGCA GGGACCCTTG AATGAGGTGC TGACGAAGAA CATGTACAGG 
GCTTTGGTGG CAGAGCATTT AAAGGGATGG TACAACCGCA GTGCTGGGCA TAGGGACCCA 
GGCACCTATC AGCTGGTGTA CACCTACAAG TATGACCGCG GTTCCCAGCA CAGCTTGGGC 
TACCAGACGC TGCCCGCACC CTTCAGCCAC TCCAGCCGGA CCAACTCCTT CTCCTCAGTG 
TCGTCGACTG CTAGCGGAGG GGGAAACTGG CAGAATCAGT TAGCAGTGGG TCTGGCGGAT 
GGAGAACTGC CGGACGGTCC CTTGAGCGCT GGGGCTTATA GTCCTGCATA CAGCCGCAGT 
CCAACACACA GCAGCCGGTT CCCATCAGAA GGAGCGGCGT CTCACACTTC AGAGCCACTG 
GAGGTGTTTG GAGCCGTGGC CAGCTGCTCC GACACAGAAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294837.1
CDS	94..3198
Translation	MKEQRSGSAERVICVCVCVCSVTRLCNPGVRAAMVWGLVCVCVCVFRAAGFIWRLVFWAVGRMRCCQESVQTCFYLRHVYQMTEGRLCQVQLLDDRKLEMLVQPKLLSRELLDLVSSHFNLKEKEYFGFTYVDDTGQCKWLQLERRVLEHDFSKKSGPIALQFLVRFYIESISLLKEHTTVELFFLNAKSAIYNGDIEVESELVFKLAAHALQESKGDYTSDENTRADLKKFPVLPTKVLKEHPSLAYCEERVMEQYRLLKGISRGQAIVQYMTLVQSLPTYGVHYYEVKDKQGMPWWLGISYKGIGQYDHQDKVKPRKLFQWKQLENLYFREKKFAVEVNDPHRRAVTKRTFGQTGLVIHTWYATHSLIKTIWVMAISQHQFYLNRKQSKAKITTARSLGDIAVDLTENGASKMSKLSAGVKNQLIMASNGSLVSTGSADSEMNDEQKKEKLAELRKKERDIQDILSQKLTELKEICLREAELTGKLPKEYPLIKGETPPHVRRRVGTAFKLDDLFPYNEDPYLRNLESRFALQQKIVEAAKKLAAEPDISKLVRKKRRRNCLDAMQKLQEIEDEMNQYRLKKGQKPTQRASVIIADDGGQRPRSRSVQCSPCLSPPLSSSGLDFDPERRSSSSDTHHDRPLNRLDHEVQEPGHYYTPREVFSAQSSPCKTLSRMPKDPRSLPQTPVLPRNAYSSSQLRSDGHIRAFRHRSGSLESQSQLFHSHCANKSVFPLSPSRRSNSTEVLDDCSSYTSMSSMDFCLPCSATPPYRTLDSHSHDYRLHRKVEIYGNTGSMPNLVPQNSSSYQQNHYGPTAYYVAGYAGFDYEPCSNGAYVYESEIEGHYNINPTYPAHGYQGRGRNRHYGIEGLDNLSQNPYATVRPPRGRQGPLNEVLTKNMYRALVAEHLKGWYNRSAGHRDPGTYQLVYTYKYDRGSQHSLGYQTLPAPFSHSSRTNSFSSVSSTASGGGNWQNQLAVGLADGELPDGPLSAGAYSPAYSRSPTHSSRFPSEGAASHTSEPLEVFGAVASCSDTEN

Target ORF information:

RefSeq Version	XM_009296562.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio FERM domain containing 4Bb (frmd4bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296562.2

ORF Insert Sequence:

ATGAAGGAGC AAAGATCAGG ATCTGCTGAA AGAGTGATCT GTGTGTGTGT GTGTGTGTGC 
AGTGTGACCC GCTTGTGTAA TCCGGGCGTC AGAGCGGCCA TGGTTTGGGG TTTAGTGTGT 
GTGTGTGTTT GTGTGTTTCG GGCCGCCGGC TTCATCTGGC GGCTGGTTTT CTGGGCTGTT 
GGGAGGATGA GGTGCTGTCA GGAGAGTGTG CAGACCTGCT TCTACCTGCG ACACGTCTAT 
CAGATGACAG AGGGACGCCT GTGCCAGGTG CAGCTGCTGG ACGACAGAAA GCTGGAGATG 
TTGGTTCAGC CCAAGCTGTT GTCTCGTGAA CTCCTGGATT TGGTCTCATC ACATTTCAAC 
CTCAAAGAGA AGGAATACTT TGGCTTTACC TATGTGGACG ACACTGGTCA GTGTAAGTGG 
TTACAGCTGG AGCGCAGAGT TCTGGAGCAC GACTTCAGCA AGAAATCAGG CCCCATCGCC 
CTTCAGTTCC TGGTCAGGTT TTACATAGAG AGCATTTCAC TTCTGAAGGA GCACACGACC 
GTGGAGCTGT TCTTTCTGAA CGCCAAGTCA GCCATCTATA ATGGTGACAT CGAGGTGGAG 
AGTGAGCTGG TTTTTAAATT AGCAGCTCAT GCATTGCAGG AATCTAAAGG AGACTATACA 
AGTGATGAAA ACACCAGAGC AGACCTGAAG AAATTTCCAG TACTGCCCAC TAAAGTGCTG 
AAGGAGCATC CGTCACTGGC ATACTGTGAG GAGCGCGTGA TGGAGCAGTA CAGGCTGTTG 
AAGGGGATCT CCAGAGGACA GGCCATAGTG CAGTATATGA CACTGGTGCA GTCACTGCCC 
ACATACGGAG TGCATTATTA TGAAGTCAAG GATAAGCAGG GTATGCCGTG GTGGCTGGGC 
ATCAGTTATA AAGGCATTGG ACAGTACGAC CACCAGGATA AAGTAAAACC CCGAAAGCTC 
TTCCAGTGGA AACAGCTGGA AAACCTGTAT TTCAGAGAGA AGAAGTTTGC TGTGGAAGTC 
AATGACCCTC ACAGGCGAGC AGTGACTAAG CGCACGTTTG GTCAGACGGG ACTGGTGATC 
CACACTTGGT ACGCGACTCA CTCCCTGATC AAAACCATCT GGGTCATGGC CATCAGTCAG 
CACCAGTTCT ACCTCAACAG AAAACAGAGC AAAGCAAAAA TCACCACAGC CAGAAGTCTA 
GGAGACATCG CAGTGGATCT GACGGAGAAC GGAGCATCGA AGATGAGCAA ACTGAGCGCT 
GGAGTGAAGA ACCAGCTCAT CATGGCCAGC AACGGCAGCC TGGTTTCCAC AGGTTCGGCT 
GATTCAGAAA TGAACGACGA GCAGAAAAAA GAGAAGCTTG CGGAGCTGAG AAAGAAGGAG 
CGAGACATTC AGGACATACT AAGCCAAAAA CTGACCGAGC TGAAGGAAAT ATGCCTGCGG 
GAAGCCGAAC TCACTGGCAA ACTCCCGAAA GAGTATCCGC TTATTAAAGG AGAAACCCCT 
CCTCATGTGC GCAGGAGAGT CGGGACGGCC TTTAAACTGG ACGACCTTTT CCCATATAAT 
GAGGACCCGT ATCTCAGAAA TCTGGAGAGC CGCTTTGCAT TGCAGCAGAA GATCGTGGAG 
GCGGCCAAGA AGCTCGCGGC GGAGCCTGAC ATCTCCAAAT TAGTGCGGAA AAAACGACGG 
AGGAACTGTC TGGACGCCAT GCAGAAACTG CAGGAGATCG AAGATGAAAT GAACCAATAC 
AGGCTGAAGA AAGGACAGAA ACCCACGCAG AGAGCCTCTG TGATCATCGC CGATGATGGC 
GGTCAGAGGC CGCGTTCTCG ATCAGTGCAG TGTTCTCCAT GTCTCAGTCC TCCTCTGTCT 
TCATCCGGGC TGGATTTCGA CCCCGAGAGA CGATCTTCAT CCAGCGACAC ACACCACGAC 
AGACCTCTGA ACAGATTAGA TCATGAGGTG CAAGAGCCTG GACATTATTA TACCCCTCGA 
GAGGTTTTCT CCGCCCAGAG CAGTCCCTGT AAAACTTTAT CCAGAATGCC AAAGGATCCC 
CGCAGTTTAC CACAGACCCC AGTCCTGCCC CGTAATGCCT ACAGCAGCAG TCAACTCCGG 
TCAGACGGTC ACATCCGAGC TTTCCGGCAT CGCAGTGGCA GTTTGGAGTC TCAGTCACAA 
CTTTTCCATT CTCACTGTGC AAACAAGTCT GTCTTCCCGC TGTCTCCCTC CCGCCGCAGC 
AACAGCACTG AGGTGTTGGA CGACTGCTCC TCCTATACCA GCATGTCCAG CATGGACTTC 
TGCCTCCCCT GCTCGGCTAC TCCTCCTTAC CGCACCCTGG ACTCACACTC CCACGACTAC 
CGGCTCCACC GCAAAGTGGA GATCTATGGC AATACAGGCA GCATGCCCAA CCTGGTCCCT 
CAAAACTCCA GTTCCTACCA GCAGAACCAC TACGGACCAA CTGCTTACTA CGTCGCTGGG 
TATGCTGGCT TCGATTATGA GCCTTGTTCC AATGGAGCTT ATGTTTACGA GAGCGAAATA 
GAGGGTCACT ATAACATCAA TCCCACTTAC CCTGCACATG GCTACCAGGG CCGGGGTCGC 
AACAGGCATT ATGGAATAGA AGGTTTGGAT AATTTGTCAC AGAATCCATA TGCCACCGTT 
AGGCCCCCTA GAGGAAGGCA GGGACCCTTG AATGAGGTGC TGACGAAGAA CATGTACAGG 
GCTTTGGTGG CAGAGCATTT AAAGGGATGG TACAACCGCA GTGCTGGGCA TAGGGACCCA 
GGCACCTATC AGCTGGTGTA CACCTACAAG TATGACCGCG GTTCCCAGCA CAGCTTGGGC 
TACCAGACGC TGCCCGCACC CTTCAGCCAC TCCAGCCGGA CCAACTCCTT CTCCTCAGTG 
TCGTCGACTG CTAGCGGAGG GGGAAACTGG CAGAATCAGT TAGCAGTGGG TCTGGCGGAT 
GGAGAACTGC CGGACGGTCC CTTGAGCGCT GGGGCTTATA GTCCTGCATA CAGCCGCAGT 
CCAACACACA GCAGCCGGTT CCCATCAGAA GGAGCGGCGT CTCACACTTC AGAGCCACTG 
GAGGTGTTTG GAGCCGTGGC CAGCTGCTCC GACACAGAAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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frmd4bb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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サポート

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