selenoe cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenoe
Entrez Gene ID	100329562
Full Name	selenoprotein e
Synonyms	fep15
General protein information	Preferred Names selenoprotein e Names selenoprotein E fish selenoprotein 15 selenoprotein 15
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene is a member of the SEP15 family. It has been detected only in fish, and thought to have arisen by gene duplication. Like Sep15, this protein is localized in the endoplasmic reticulum. Its exact function is not known; however, because of its restricted occurrence, it may have a specialized function in fish. The encoded protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Sep 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001195784.2	NP_001182713.2	fep15 selenoprotein precursor
NM_001195784.3	NP_001182713.2	selenoprotein E precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC101733398	XP_004910526.1
Danio rerio (zebrafish)	fep15	NP_001182713.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Identification and characterization of Fep15, a new selenocysteine-containing member of the Sep15 protein family.
Novoselov SV, Hua D, Lobanov AV, Gladyshev VN
The Biochemical journal394(Pt 3)575-9(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The isolation and characterization of Fep15, a D. rerio protein related to members of the Sep15 family, are reported.
Title: Identification and characterization of Fep15, a new selenocysteine-containing member of the Sep15 protein family.

The following selenoe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenoe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36911	NM_001195784.2 Latest version!	Danio rerio fep15 selenoprotein (fep15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa141171	NM_001195784.3 Latest version!	Danio rerio selenoprotein e (selenoe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenoe ( NM_001195784.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001182713.2 Danio rerio fep15 selenoprotein (fep15), mRNA.

Price & Availability↑

CloneID	ODa36911
Clone ID Related Accession (Same CDS sequence)	NM_001195784.2
Accession Version	NM_001195784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(Zebrafish)
Product	fep15 selenoprotein precursor
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH506255.1, AL913326.1 and EH504865.1. On or before Dec 19, 2010 this sequence version replaced XM_002667156.1, NM_001195784.1. ##Evidence-Data-START## Transcript exon combination :: EH504865.1, CR927449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA708828, SAMEA898401 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 16236027 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTCA CACTGGTGGC CCTCCTGGCT CTATGTGCCA CTGGGAGAAC AGCTGAAAAT 
CTTTCAGAAA GCACCACTGA CCAGGACAAG CTTGTTATAG CCAGAGGAAA GCTGGTGGCT 
CCCAGTGTAG TAGGATGATC CATAAAGAAG ATGCCAGAGC TCTACAATTT CCTGATGGAG 
CGATGGGCAT TATATCATAA CCTGGAGTAT GATTCAGGGG AGGATAAAAA CCCGCGCCTC 
ATCTTCTACA ACGACAAGGA TGAGGAAGTC AAGGTTGTTC CTGTGAAGAA GATGAAAGCA 
GATGAGATCA GCAGTTTGCT GGATTCTCTG GGTTTCTACA AGAGGTCAGA GAAAGGCGAG 
GAGGTCCCGG AGGAGTTCAA ACATTTCCCT CTCAGAGCGC CCCGGGACGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182713.2
CDS	53..469
Translation	MWLTLVALLALCATGRTAENLSESTTDQDKLVIARGKLVAPSVVG*SIKKMPELYNFLMERWALYHNLEYDSGEDKNPRLIFYNDKDEEVKVVPVKKMKADEISSLLDSLGFYKRSEKGEEVPEEFKHFPLRAPRDEL

Target ORF information:

RefSeq Version	NM_001195784.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fep15 selenoprotein (fep15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195784.2

ORF Insert Sequence:

ATGTGGCTCA CACTGGTGGC CCTCCTGGCT CTATGTGCCA CTGGGAGAAC AGCTGAAAAT 
CTTTCAGAAA GCACCACTGA CCAGGACAAG CTTGTTATAG CCAGAGGAAA GCTGGTGGCT 
CCCAGTGTAG TAGGATGATC CATAAAGAAG ATGCCAGAGC TCTACAATTT CCTGATGGAG 
CGATGGGCAT TATATCATAA CCTGGAGTAT GATTCAGGGG AGGATAAAAA CCCGCGCCTC 
ATCTTCTACA ACGACAAGGA TGAGGAAGTC AAGGTTGTTC CTGTGAAGAA GATGAAAGCA 
GATGAGATCA GCAGTTTGCT GGATTCTCTG GGTTTCTACA AGAGGTCAGA GAAAGGCGAG 
GAGGTCCCGG AGGAGTTCAA ACATTTCCCT CTCAGAGCGC CCCGGGACGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

selenoe ( NM_001195784.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001182713.2 Danio rerio selenoprotein e (selenoe), mRNA.

Price & Availability↑

CloneID	ODa141171
Clone ID Related Accession (Same CDS sequence)	NM_001195784.3
Accession Version	NM_001195784.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Danio rerio(zebrafish)
Product	selenoprotein E precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CABZ01077128.1. On Aug 5, 2017 this sequence version replaced NM_001195784.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01003219.1, GDQQ01002183.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 16236027 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTCA CACTGGTGGC CCTCCTGGCT CTATGTGCCA CTGGGAGAAC AGCTGAAAAT 
CTTTCAGAAA GCACCACTGA CCAGGACAAG CTTGTTATAG CCAGAGGAAA GCTGGTGGCT 
CCCAGTGTAG TAGGATGATC CATAAAGAAG ATGCCAGAGC TCTACAATTT CCTGATGGAG 
CGATGGGCAT TATATCATAA CCTGGAGTAT GATTCAGGGG AGGATAAAAA CCCGCGCCTC 
ATCTTCTACA ACGACAAGGA TGAGGAAGTC AAGGTTGTTC CTGTGAAAAA GATGAAAGCA 
GATGAGATCA GCAGTTTGCT GGATTCTCTG GGTTTCTACA AGAGGTCAGA GAAAGGCGAG 
GAGGTCCCGG AGGAGTTCAA ACATTTCCCC CTCAGAGCGC CCCGGGACGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182713.2
CDS	75..491
Translation	MWLTLVALLALCATGRTAENLSESTTDQDKLVIARGKLVAPSVVG*SIKKMPELYNFLMERWALYHNLEYDSGEDKNPRLIFYNDKDEEVKVVPVKKMKADEISSLLDSLGFYKRSEKGEEVPEEFKHFPLRAPRDEL

Target ORF information:

RefSeq Version	NM_001195784.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein e (selenoe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195784.3

ORF Insert Sequence:

ATGTGGCTCA CACTGGTGGC CCTCCTGGCT CTATGTGCCA CTGGGAGAAC AGCTGAAAAT 
CTTTCAGAAA GCACCACTGA CCAGGACAAG CTTGTTATAG CCAGAGGAAA GCTGGTGGCT 
CCCAGTGTAG TAGGATGATC CATAAAGAAG ATGCCAGAGC TCTACAATTT CCTGATGGAG 
CGATGGGCAT TATATCATAA CCTGGAGTAT GATTCAGGGG AGGATAAAAA CCCGCGCCTC 
ATCTTCTACA ACGACAAGGA TGAGGAAGTC AAGGTTGTTC CTGTGAAAAA GATGAAAGCA 
GATGAGATCA GCAGTTTGCT GGATTCTCTG GGTTTCTACA AGAGGTCAGA GAAAGGCGAG 
GAGGTCCCGG AGGAGTTCAA ACATTTCCCC CTCAGAGCGC CCCGGGACGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Identification and characterization of Fep15, a new selenocysteine-containing member of the Sep15 protein family.
The Biochemical journal394(Pt 3)575-9(2006 Mar)
Novoselov SV,Hua D,Lobanov AV,Gladyshev VN

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selenoe cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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