si:ch211-281g13.5 cDNA ORF clone, Danio rerio(zebrafish)

The following si:ch211-281g13.5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-281g13.5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa32486 NM_001145631.1
Latest version!
Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 5.4 (herc5.4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa06149 XM_005160109.4
Latest version!
Danio rerio si:ch211-281g13.5 (si:ch211-281g13.5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa53658 XM_021475845.1
Latest version!
Danio rerio si:ch211-281g13.5 (si:ch211-281g13.5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa53658 XM_017355671.1
Latest version!
Danio rerio si:ch211-281g13.5 (si:ch211-281g13.5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa32486
    Clone ID Related Accession (Same CDS sequence) NM_001145631.1
    Accession Version NM_001145631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-05
    Organism Danio rerio(zebrafish)
    Product probable E3 ubiquitin-protein ligase HERC4-like
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX321880.9. On or before Mar 5, 2009 this sequence version replaced XM_001921778.1, XM_001921770.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGTGTT ATTGGGGAGC GCAGGTCAGA GAAGGATTCG GTCTGGTTAA ACCTGAGCAG 
    GTCAGACATG GAAACTGGGG AATAAAATTC TGTCCGAGTT CAGCAGTTCA GGACATCTCA
    GCTGGAAAGA GTTGTATTGG ATTCATCAGA GAGGGGAAGG TGTCGGTCCT GAGACTGCAC
    AATGAAGATG CTGATGAAAA ACTAGAACCA TTACAGCTAC AGAATGAGAG AATCAGATCT
    ATTGTCTGTG GGGAAGCTGG TGCAGTTCTT CTTGCTGATG ATGGAAAAGT TCTCATTATG
    GACCAATCTA CAGTGTGCAG ACCTCTTAAA AGTCTGGAGA ACAGGCAGGT GATTCAGATC
    GCATGTGGAG ACCAGCATTC AATGGCGCTG AGCAATGATG GTCAGTTGTT CGTTTGGGGC
    GAGAACACTC ATGGTCAGCT GGGTTTGAGG AAAGAGCAGG CCGGCACTCA GTCTCCTCAA
    CACCTCCAGA GTCTGTGTGA GATCCCAGTG GCTCAGATCA GCGCTGGAGG AAACCACAGC
    TTTGTGTTGT CTCTGTCAGG AGTCGTGTTT GGGTGGGGGA GCAACTCTGC TGGACAGCTG
    GGTCTCGGAG ACACTAAAGA CAGATTCGTC CCTACAATTG TTAAAAGCTT GTGTGGAAAG
    AAAACAGTGT CCATCTCATG TGGAGGAGAA CACACTGCCA CTCTGTCAAA GGGAGGAACA
    GTGTTCACAT TTGGATCAGG AGGTTTTGGT CAGCTTGGGC ACAACTCACT TAAAGATGAA
    CATCATCCAC GACTGATTGC TGAACTGTGG GGATCTAAAG TGTCGCAGGT CACATGTGGG
    AGACATCACA CGCTTGTATT TGAGGAATCG TCAAAGCTGA TCTACTCATT TGGATGTGGG
    ATGCAAGGGC AGCTGGGAAA TGGAGAACAG GTCAAGCAGT CTGTGCCGTT ACCAGTGCTT
    CTGCCAACTG AATGTACTGA ATTTACGATG GAAAAACTCA TCACTGGAGA ACACCACTCC
    TTTGCCTTGT TTTTCAAGAA TCCTGGAAAT GAGTCTGAAA AGCCTAAATC TGGTTCAAAT
    GGAGGAATTT TGACACTAAA TGACAGAATG ATTGACCGCT GGGTGTCTGA AAGTGACCCA
    TGGCCAACAG TAAAGAATGA AATAAACACA GTGTTCTCCT CCGCTGCCTC ACTGAATGGA
    AGTTTTCTCA AAACAAGTCG CGATGAACAT TATCAGACGT CTGTGGAGCA TTGTGGCCTG
    GATTTTGATC TGGTTAAAAC ATCCTTTGCA AAGTTATTCG CAAGTAAAAG TCTGATTTCA
    GAGGTGGTTC AGCAGACGCT GCTGCCATCT CTGAATCCAA ACCCAACTGG TGTGGAAGCT
    CTGAGACTTT ATCTTCTCCT CCCTGAGCTC ATCAATGGTC TCAAGAAACA AAGAACCAAA
    CTCATTGAAG CTCTGGCCTC CAAAATCCTT GAGCTGAATT CTGCAGCTCG CAAGGTGCTA
    GAGATGTATT GGTCCAGACT CCCTGATGAT TGGTTTGAAC GCTTAATAAA GTTATTTCAC
    AAAGAATCCA CTGATCTGAT TGACCAGATT TGTGATGAAA CTGACCATCA GTCAACCAGA
    CACCTGAAGA ACATTTTGGA GATCCTTCAG ATGGCCTATC AGATCAGTTG CAGTACCGAC
    ATTACGCATC ATGATTTCAT CATTCATAAG ATCAACGATC TCCTAGACCT ACTGCAAACC
    ATCACGGCAG ACCTGCTCAG AATGGAATTA TACATGCAAA TCAACGAAGA CTTCCATGAT
    ATGGTGAAAC TGGAAGAGTA CTACTTTAGA ACAGTTAAAA TATTGGTCAA GTTCCCTTTT
    GCGTCTGACT TTATAACTAA ACAGAGAATG TTTAATCTCT TGCAGGAGGA ATGTTTTCGG
    AACGTACCAA ACCTGGCAAT GGGAAATAGT TTTAGCAACT TGTTGCACAT AAACAGGGAA
    TCAGTGCTGA CGGACACCCT TCAGTATCTT AGACAAAACA CTCACTCGTT TATGCATCCA
    TTGCAGGTGG CGTTCATCGG GGAGGATGGA ATAGATATGA GGGGTTTATC AGCAGAATTC
    TTCTCCCTTA TCTCCAAGTC TTTTATTGAA TGGGATAAAA AGATTCTGGA AGTTCATGAG
    AGTTCACTGG TTTGGTTCAA TCCTCATCAT ACACGGGGCA ATAAGAACTT CTACTATCTT
    GGAGTGATCT GTGGAATGGC GCTCTACAAC AGACATCACA TCAACATTGA CTTTCCACTG
    GCCTTATTCA AGAAACTCCT CCAGCAGAAG CCCAGTCTGG ATGATCTGGA GGAGCTCTCA
    CCTGTGGAGG CCAGAAGTCT GAAAAACCTT CTTGAGGAGG ACGAGGAGGA AGTAGTGAAT
    TTGCAACATT TGGATTTCAC GTGCAAAGGG CAAGAACTCG TCCCAAATGG AAGTCAAATT
    CAAGTAAACA AAGTTAACAG ACAGAAGTAT GTGGATTTGT ATGTTGATCT TGAGTTCAAC
    AAGTCTGTGA AGACTCAGTT TGAGCAGTTT ACAAGAGGTT TCTCTAAGGG CTGCCCGCTC
    GATGCGTGGA CCATGTTTCA CCCTGAAGAG CTGCAGGAGC TTCTCCATGG CTCTCCTCAA
    TATAACTGGA AGGAGCTACA ACAGTCGGCT TCCTATGAAG GCTGCTCTGC CTCTGATGAG
    CTCATCAAGA ACTTCTGGAC TGTTTTCTTT GAGCTCTCTG AAGAGAACAA GAAGAAATTT
    CTGATGTTCT TGTATGGAAC GGAGCGTGTA CCTGCTGGAG GTTTGTCAAA ACGTGCTCTG
    AAGATTTCTC AGACAGACTG TCCTGATCCA GATGACCATT TGCCAGAAGC CCAGACCTGT
    TTTGAAAGAT TAGTTCTTCC AAAATACAGT GATATCAACA CACTTCACGA TAAGTTAATA
    CATGCAATCA ACTCTTGTGC TGTTTTTGGA CGAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139103.1
    CDS78..3056
    Translation

    Target ORF information:

    RefSeq Version NM_001145631.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 5.4 (herc5.4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGTGTT ATTGGGGAGC GCAGGTCAGA GAAGGATTCG GTCTGGTTAA ACCTGAGCAG 
    GTCAGACATG GAAACTGGGG AATAAAATTC TGTCCGAGTT CAGCAGTTCA GGACATCTCA
    GCTGGAAAGA GTTGTATTGG ATTCATCAGA GAGGGGAAGG TGTCGGTCCT GAGACTGCAC
    AATGAAGATG CTGATGAAAA ACTAGAACCA TTACAGCTAC AGAATGAGAG AATCAGATCT
    ATTGTCTGTG GGGAAGCTGG TGCAGTTCTT CTTGCTGATG ATGGAAAAGT TCTCATTATG
    GACCAATCTA CAGTGTGCAG ACCTCTTAAA AGTCTGGAGA ACAGGCAGGT GATTCAGATC
    GCATGTGGAG ACCAGCATTC AATGGCGCTG AGCAATGATG GTCAGTTGTT CGTTTGGGGC
    GAGAACACTC ATGGTCAGCT GGGTTTGAGG AAAGAGCAGG CCGGCACTCA GTCTCCTCAA
    CACCTCCAGA GTCTGTGTGA GATCCCAGTG GCTCAGATCA GCGCTGGAGG AAACCACAGC
    TTTGTGTTGT CTCTGTCAGG AGTCGTGTTT GGGTGGGGGA GCAACTCTGC TGGACAGCTG
    GGTCTCGGAG ACACTAAAGA CAGATTCGTC CCTACAATTG TTAAAAGCTT GTGTGGAAAG
    AAAACAGTGT CCATCTCATG TGGAGGAGAA CACACTGCCA CTCTGTCAAA GGGAGGAACA
    GTGTTCACAT TTGGATCAGG AGGTTTTGGT CAGCTTGGGC ACAACTCACT TAAAGATGAA
    CATCATCCAC GACTGATTGC TGAACTGTGG GGATCTAAAG TGTCGCAGGT CACATGTGGG
    AGACATCACA CGCTTGTATT TGAGGAATCG TCAAAGCTGA TCTACTCATT TGGATGTGGG
    ATGCAAGGGC AGCTGGGAAA TGGAGAACAG GTCAAGCAGT CTGTGCCGTT ACCAGTGCTT
    CTGCCAACTG AATGTACTGA ATTTACGATG GAAAAACTCA TCACTGGAGA ACACCACTCC
    TTTGCCTTGT TTTTCAAGAA TCCTGGAAAT GAGTCTGAAA AGCCTAAATC TGGTTCAAAT
    GGAGGAATTT TGACACTAAA TGACAGAATG ATTGACCGCT GGGTGTCTGA AAGTGACCCA
    TGGCCAACAG TAAAGAATGA AATAAACACA GTGTTCTCCT CCGCTGCCTC ACTGAATGGA
    AGTTTTCTCA AAACAAGTCG CGATGAACAT TATCAGACGT CTGTGGAGCA TTGTGGCCTG
    GATTTTGATC TGGTTAAAAC ATCCTTTGCA AAGTTATTCG CAAGTAAAAG TCTGATTTCA
    GAGGTGGTTC AGCAGACGCT GCTGCCATCT CTGAATCCAA ACCCAACTGG TGTGGAAGCT
    CTGAGACTTT ATCTTCTCCT CCCTGAGCTC ATCAATGGTC TCAAGAAACA AAGAACCAAA
    CTCATTGAAG CTCTGGCCTC CAAAATCCTT GAGCTGAATT CTGCAGCTCG CAAGGTGCTA
    GAGATGTATT GGTCCAGACT CCCTGATGAT TGGTTTGAAC GCTTAATAAA GTTATTTCAC
    AAAGAATCCA CTGATCTGAT TGACCAGATT TGTGATGAAA CTGACCATCA GTCAACCAGA
    CACCTGAAGA ACATTTTGGA GATCCTTCAG ATGGCCTATC AGATCAGTTG CAGTACCGAC
    ATTACGCATC ATGATTTCAT CATTCATAAG ATCAACGATC TCCTAGACCT ACTGCAAACC
    ATCACGGCAG ACCTGCTCAG AATGGAATTA TACATGCAAA TCAACGAAGA CTTCCATGAT
    ATGGTGAAAC TGGAAGAGTA CTACTTTAGA ACAGTTAAAA TATTGGTCAA GTTCCCTTTT
    GCGTCTGACT TTATAACTAA ACAGAGAATG TTTAATCTCT TGCAGGAGGA ATGTTTTCGG
    AACGTACCAA ACCTGGCAAT GGGAAATAGT TTTAGCAACT TGTTGCACAT AAACAGGGAA
    TCAGTGCTGA CGGACACCCT TCAGTATCTT AGACAAAACA CTCACTCGTT TATGCATCCA
    TTGCAGGTGG CGTTCATCGG GGAGGATGGA ATAGATATGA GGGGTTTATC AGCAGAATTC
    TTCTCCCTTA TCTCCAAGTC TTTTATTGAA TGGGATAAAA AGATTCTGGA AGTTCATGAG
    AGTTCACTGG TTTGGTTCAA TCCTCATCAT ACACGGGGCA ATAAGAACTT CTACTATCTT
    GGAGTGATCT GTGGAATGGC GCTCTACAAC AGACATCACA TCAACATTGA CTTTCCACTG
    GCCTTATTCA AGAAACTCCT CCAGCAGAAG CCCAGTCTGG ATGATCTGGA GGAGCTCTCA
    CCTGTGGAGG CCAGAAGTCT GAAAAACCTT CTTGAGGAGG ACGAGGAGGA AGTAGTGAAT
    TTGCAACATT TGGATTTCAC GTGCAAAGGG CAAGAACTCG TCCCAAATGG AAGTCAAATT
    CAAGTAAACA AAGTTAACAG ACAGAAGTAT GTGGATTTGT ATGTTGATCT TGAGTTCAAC
    AAGTCTGTGA AGACTCAGTT TGAGCAGTTT ACAAGAGGTT TCTCTAAGGG CTGCCCGCTC
    GATGCGTGGA CCATGTTTCA CCCTGAAGAG CTGCAGGAGC TTCTCCATGG CTCTCCTCAA
    TATAACTGGA AGGAGCTACA ACAGTCGGCT TCCTATGAAG GCTGCTCTGC CTCTGATGAG
    CTCATCAAGA ACTTCTGGAC TGTTTTCTTT GAGCTCTCTG AAGAGAACAA GAAGAAATTT
    CTGATGTTCT TGTATGGAAC GGAGCGTGTA CCTGCTGGAG GTTTGTCAAA ACGTGCTCTG
    AAGATTTCTC AGACAGACTG TCCTGATCCA GATGACCATT TGCCAGAAGC CCAGACCTGT
    TTTGAAAGAT TAGTTCTTCC AAAATACAGT GATATCAACA CACTTCACGA TAAGTTAATA
    CATGCAATCA ACTCTTGTGC TGTTTTTGGA CGAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06149
    Clone ID Related Accession (Same CDS sequence) XM_005160109.4
    Accession Version XM_005160109.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product probable E3 ubiquitin-protein ligase HERC4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160109.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGTGTT ATTGGGGAGC GCAGGTCAGA GAAGGATTCG GTCTGGTTAA ACCTGAGCAG 
    GTCAGACATG GAAACTGGGG AATAAAATTC TGTCCGAGTT CAGCAGTTCA GGACATCTCA
    GCTGGAAAGA GTTGTATTGG ATTCATCAGA GAGGGGAAGG TGTCGGTCCT GAGACTGCAC
    AATGAAGATG CTGATGAAAA ACTAGAACCA TTACAGCTAC AGAATGAGAG AATCAGATCT
    ATTGTCTGTG GGGAAGCTGG TGCAGTTCTT CTTGCTGATG ATGGAAAAGT TCTCATTATG
    GACCAATCTA CAGTGTGCAG ACCTCTTAAA AGTCTGGAGA ACAGGCAGGT GATTCAGATC
    GCATGTGGAG ACCAGCATTC AATGGCGCTG AGCAATGATG GTCAGTTGTT CGTTTGGGGC
    GAGAACACTC ATGGTCAGCT GGGTTTGAGG AAAGAGCAGG CCGGCACTCA GTCTCCTCAA
    CACCTCCAGA GTCTGTGTGA GATCCCAGTG GCTCAGATCA GCGCTGGAGG AAACCACAGC
    TTTGTGTTGT CTCTGTCAGG AGTCGTGTTT GGGTGGGGGA GCAACTCTGC TGGACAGCTG
    GGTCTCGGAG ACACTAAAGA CAGATTCGTC CCTACAATTG TTAAAAGCTT GTGTGGAAAG
    AAAACAGTGT CCATCTCATG TGGAGGAGAA CACACTGCCA CTCTGTCAAA GGGAGGAACA
    GTGTTCACAT TTGGATCAGG AGGTTTTGGT CAGCTTGGGC ACAACTCACT TAAAGATGAA
    CATCATCCAC GACTGATTGC TGAACTGTGG GGATCTAAAG TGTCGCAGGT CACATGTGGG
    AGACATCACA CGCTTGTATT TGAGGAATCG TCAAAGCTGA TCTACTCATT TGGATGTGGG
    ATGCAAGGGC AGCTGGGAAA TGGAGAACAG GTCAAGCAGT CTGTGCCGTT ACCAGTGCTT
    CTGCCAACTG AATGTACTGA ATTTACGATG GAAAAACTCA TCACTGGAGA ACACCACTCC
    TTTGCCTTGT TTTTCAAGAA TCCTGGAAAT GAGTCTGAAA AGCCTAAATC TGGTTCAAAT
    GGAGGAATTT TGACACTAAA TGACAGAATG ATTGACCGCT GGGTGTCTGA AAGTGACCCA
    TGGCCAACAG TAAAGAATGA AATAAACACA GTGTTCTCCT CCGCTGCCTC ACTGAATGGA
    AGTTTTCTCA AAACAAGTCG CGATGAACAT TATCAGACGT CTGTGGAGCA TTGTGGCCTG
    GATTTTGATC TGGTTAAAAC ATCCTTTGCA AAGTTATTCG CAAGTAAAAG TCTGATTTCA
    GAGGTGGTGA AGGTGGTTCA GCAGACGCTG CTGCCATCTC TGAATCCAAA CCCAACTGGT
    GTGGAAGCTC TGAGACTTTA TCTTCTCCTC CCTGAGCTCA TCAATGGTCT CAAGAAACAA
    AGAACCAAAC TCATTGAAGC TCTGGCCTCC AAAATCCTTG AGCTGAATTC TGCAGCTCGC
    AAGGTGCTAG AGATGTATTG GTCCAGACTC CCTGATGATT GGTTTGAACG CTTAATAAAG
    TTATTTCACA AAGAATCCAC TGATCTGATT GACCAGATTT GTGATGAAAC TGACCATCAG
    TCAACCAGAC ACCTGAAGAA CATTTTGGAG ATCCTTCAGA TGGCCTATCA GATCAGTTGC
    AGTACCGACA TTACGCATCA TGATTTCATC ATTCATAAGA TCAACGATCT CCTAGACCTA
    CTGCAAACCA TCACGGCAGA CCTGCTCAGA ATGGAATTAT ACATGCAAAT CAACGAAGAC
    TTCCATGATA TGGTGAAACT GGAAGAGTAC TACTTTAGAA CAGTTAAAAT ATTGGTCAAG
    TTCCCTTTTG CGTCTGACTT TATAACTAAA CAGAGAATGT TTAATCTCTT GCAGGAGGAA
    TGTTTTCGGA ACGTACCAAA CCTGGCAATG GGAAATAGTT TTAGCAACTT GTTGCACATA
    AACAGGGAAT CAGTGCTGAC GGACACCCTT CAGTATCTTA GACAAAACAC TCACTCGTTT
    ATGCATCCAT TGCAGGTGGC GTTCATCGGG GAGGATGGAA TAGATATGAG GGGTTTATCA
    GCAGAATTCT TCTCCCTTAT CTCCAAGTCT TTTATTGAAT GGGATAAAAA GATTCTGGAA
    GTTCATGAGA GTTCACTGGT TTGGTTCAAT CCTCATCATA CACGGGGCAA TAAGAACTTC
    TACTATCTTG GAGTGATCTG TGGAATGGCG CTCTACAACA GACATCACAT CAACATTGAC
    TTTCCACTGG CCTTATTCAA GAAACTCCTC CAGCAGAAGC CCAGTCTGGA TGATCTGGAG
    GAGCTCTCAC CTGTGGAGGC CAGAAGTCTG AAAAACCTTC TTGAGGAGGA CGAGGAGGAA
    GTAGTGAATT TGCAACATTT GGATTTCACG TGCAAAGGGC AAGAACTCGT CCCAAATGGA
    AGTCAAATTC AAGTAAACAA AGTTAACAGA CAGAAGTATG TGGATTTGTA TGTTGATCTT
    GAGTTCAACA AGTCTGTGAA GACTCAGTTT GAGCAGTTTA CAAGAGGTTT CTCTAAGGGC
    TGCCCGCTCG ATGCGTGGAC CATGTTTCAC CCTGAAGAGC TGCAGGAGCT TCTCCATGGC
    TCTCCTCAAT ATAACTGGAA GGAGCTACAA CAGTCGGCTT CCTATGAAGG CTGCTCTGCC
    TCTGATGAGC TCATCAAGAA CTTCTGGACT GTTTTCTTTG AGCTCTCTGA AGAGAACAAG
    AAGAAATTTC TGATGTTCTT GTATGGAACG GAGCGTGTAC CTGCTGGAGG TTTGTCAAAA
    CGTGCTCTGA AGATTTCTCA GACAGACTGT CCTGATCCAG ATGACCATTT GCCAGAAGCC
    CAGACCTGTT TTGAAAGATT AGTTCTTCCA AAATACAGTG ATATCAACAC ACTTCACGAT
    AAGTTAATAC ATGCAATCAA CTCTTGTGCT GTTTTTGGAC GAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160166.1
    CDS331..3318
    Translation

    Target ORF information:

    RefSeq Version XM_005160109.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-281g13.5 (si:ch211-281g13.5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160109.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGTGTT ATTGGGGAGC GCAGGTCAGA GAAGGATTCG GTCTGGTTAA ACCTGAGCAG 
    GTCAGACATG GAAACTGGGG AATAAAATTC TGTCCGAGTT CAGCAGTTCA GGACATCTCA
    GCTGGAAAGA GTTGTATTGG ATTCATCAGA GAGGGGAAGG TGTCGGTCCT GAGACTGCAC
    AATGAAGATG CTGATGAAAA ACTAGAACCA TTACAGCTAC AGAATGAGAG AATCAGATCT
    ATTGTCTGTG GGGAAGCTGG TGCAGTTCTT CTTGCTGATG ATGGAAAAGT TCTCATTATG
    GACCAATCTA CAGTGTGCAG ACCTCTTAAA AGTCTGGAGA ACAGGCAGGT GATTCAGATC
    GCATGTGGAG ACCAGCATTC AATGGCGCTG AGCAATGATG GTCAGTTGTT CGTTTGGGGC
    GAGAACACTC ATGGTCAGCT GGGTTTGAGG AAAGAGCAGG CCGGCACTCA GTCTCCTCAA
    CACCTCCAGA GTCTGTGTGA GATCCCAGTG GCTCAGATCA GCGCTGGAGG AAACCACAGC
    TTTGTGTTGT CTCTGTCAGG AGTCGTGTTT GGGTGGGGGA GCAACTCTGC TGGACAGCTG
    GGTCTCGGAG ACACTAAAGA CAGATTCGTC CCTACAATTG TTAAAAGCTT GTGTGGAAAG
    AAAACAGTGT CCATCTCATG TGGAGGAGAA CACACTGCCA CTCTGTCAAA GGGAGGAACA
    GTGTTCACAT TTGGATCAGG AGGTTTTGGT CAGCTTGGGC ACAACTCACT TAAAGATGAA
    CATCATCCAC GACTGATTGC TGAACTGTGG GGATCTAAAG TGTCGCAGGT CACATGTGGG
    AGACATCACA CGCTTGTATT TGAGGAATCG TCAAAGCTGA TCTACTCATT TGGATGTGGG
    ATGCAAGGGC AGCTGGGAAA TGGAGAACAG GTCAAGCAGT CTGTGCCGTT ACCAGTGCTT
    CTGCCAACTG AATGTACTGA ATTTACGATG GAAAAACTCA TCACTGGAGA ACACCACTCC
    TTTGCCTTGT TTTTCAAGAA TCCTGGAAAT GAGTCTGAAA AGCCTAAATC TGGTTCAAAT
    GGAGGAATTT TGACACTAAA TGACAGAATG ATTGACCGCT GGGTGTCTGA AAGTGACCCA
    TGGCCAACAG TAAAGAATGA AATAAACACA GTGTTCTCCT CCGCTGCCTC ACTGAATGGA
    AGTTTTCTCA AAACAAGTCG CGATGAACAT TATCAGACGT CTGTGGAGCA TTGTGGCCTG
    GATTTTGATC TGGTTAAAAC ATCCTTTGCA AAGTTATTCG CAAGTAAAAG TCTGATTTCA
    GAGGTGGTGA AGGTGGTTCA GCAGACGCTG CTGCCATCTC TGAATCCAAA CCCAACTGGT
    GTGGAAGCTC TGAGACTTTA TCTTCTCCTC CCTGAGCTCA TCAATGGTCT CAAGAAACAA
    AGAACCAAAC TCATTGAAGC TCTGGCCTCC AAAATCCTTG AGCTGAATTC TGCAGCTCGC
    AAGGTGCTAG AGATGTATTG GTCCAGACTC CCTGATGATT GGTTTGAACG CTTAATAAAG
    TTATTTCACA AAGAATCCAC TGATCTGATT GACCAGATTT GTGATGAAAC TGACCATCAG
    TCAACCAGAC ACCTGAAGAA CATTTTGGAG ATCCTTCAGA TGGCCTATCA GATCAGTTGC
    AGTACCGACA TTACGCATCA TGATTTCATC ATTCATAAGA TCAACGATCT CCTAGACCTA
    CTGCAAACCA TCACGGCAGA CCTGCTCAGA ATGGAATTAT ACATGCAAAT CAACGAAGAC
    TTCCATGATA TGGTGAAACT GGAAGAGTAC TACTTTAGAA CAGTTAAAAT ATTGGTCAAG
    TTCCCTTTTG CGTCTGACTT TATAACTAAA CAGAGAATGT TTAATCTCTT GCAGGAGGAA
    TGTTTTCGGA ACGTACCAAA CCTGGCAATG GGAAATAGTT TTAGCAACTT GTTGCACATA
    AACAGGGAAT CAGTGCTGAC GGACACCCTT CAGTATCTTA GACAAAACAC TCACTCGTTT
    ATGCATCCAT TGCAGGTGGC GTTCATCGGG GAGGATGGAA TAGATATGAG GGGTTTATCA
    GCAGAATTCT TCTCCCTTAT CTCCAAGTCT TTTATTGAAT GGGATAAAAA GATTCTGGAA
    GTTCATGAGA GTTCACTGGT TTGGTTCAAT CCTCATCATA CACGGGGCAA TAAGAACTTC
    TACTATCTTG GAGTGATCTG TGGAATGGCG CTCTACAACA GACATCACAT CAACATTGAC
    TTTCCACTGG CCTTATTCAA GAAACTCCTC CAGCAGAAGC CCAGTCTGGA TGATCTGGAG
    GAGCTCTCAC CTGTGGAGGC CAGAAGTCTG AAAAACCTTC TTGAGGAGGA CGAGGAGGAA
    GTAGTGAATT TGCAACATTT GGATTTCACG TGCAAAGGGC AAGAACTCGT CCCAAATGGA
    AGTCAAATTC AAGTAAACAA AGTTAACAGA CAGAAGTATG TGGATTTGTA TGTTGATCTT
    GAGTTCAACA AGTCTGTGAA GACTCAGTTT GAGCAGTTTA CAAGAGGTTT CTCTAAGGGC
    TGCCCGCTCG ATGCGTGGAC CATGTTTCAC CCTGAAGAGC TGCAGGAGCT TCTCCATGGC
    TCTCCTCAAT ATAACTGGAA GGAGCTACAA CAGTCGGCTT CCTATGAAGG CTGCTCTGCC
    TCTGATGAGC TCATCAAGAA CTTCTGGACT GTTTTCTTTG AGCTCTCTGA AGAGAACAAG
    AAGAAATTTC TGATGTTCTT GTATGGAACG GAGCGTGTAC CTGCTGGAGG TTTGTCAAAA
    CGTGCTCTGA AGATTTCTCA GACAGACTGT CCTGATCCAG ATGACCATTT GCCAGAAGCC
    CAGACCTGTT TTGAAAGATT AGTTCTTCCA AAATACAGTG ATATCAACAC ACTTCACGAT
    AAGTTAATAC ATGCAATCAA CTCTTGTGCT GTTTTTGGAC GAGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53658
    Clone ID Related Accession (Same CDS sequence) XM_017355671.1 , XM_021475845.1
    Accession Version XM_017355671.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable E3 ubiquitin-protein ligase HERC4-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCAAT CTACAGTGTG CAGACCTCTT AAAAGTCTGG AGAACAGGCA GGTGATTCAG 
    ATCGCATGTG GAGACCAGCA TTCAATGGCG CTGAGCAATG ATGGTCAGTT GTTCGTTTGG
    GGCGAGAACA CTCATGGTCA GCTGGGTTTG AGGAAAGAGC AGGCCGGCAC TCAGTCTCCT
    CAACACCTCC AGAGTCTGTG TGAGATCCCA GTGGCTCAGA TCAGCGCTGG AGGAAACCAC
    AGCTTTGTGT TGTCTCTGTC AGGAGTCGTG TTTGGGTGGG GGAGCAACTC TGCTGGACAG
    CTGGGTCTCG GAGACACTAA AGACAGATTC GTCCCTACAA TTGTTAAAAG CTTGTGTGGA
    AAGAAAACAG TGTCCATCTC ATGTGGAGGA GAACACACTG CCACTCTGTC AAAGGGAGGA
    ACAGTGTTCA CATTTGGATC AGGAGGTTTT GGTCAGCTTG GGCACAACTC ACTTAAAGAT
    GAACATCATC CACGACTGAT TGCTGAACTG TGGGGATCTA AAGTGTCGCA GGTCACATGT
    GGGAGACATC ACACGCTTGT ATTTGAGGAA TCGTCAAAGC TGATCTACTC ATTTGGATGT
    GGGATGCAAG GGCAGCTGGG AAATGGAGAA CAGGTCAAGC AGTCTGTGCC GTTACCAGTG
    CTTCTGCCAA CTGAATGTAC TGAATTTACG ATGGAAAAAC TCATCACTGG AGAACACCAC
    TCCTTTGCCT TGTTTTTCAA GAATCCTGGA AATGAGTCTG AAAAGCCTAA ATCTGGTTCA
    AATGGAGGAA TTTTGACACT AAATGACAGA ATGATTGACC GCTGGGTGTC TGAAAGTGAC
    CCATGGCCAA CAGTAAAGAA TGAAATAAAC ACAGTGTTCT CCTCCGCTGC CTCACTGAAT
    GGAAGTTTTC TCAAAACAAG TCGCGATGAA CATTATCAGA CGTCTGTGGA GCATTGTGGC
    CTGGATTTTG ATCTGGTTAA AACATCCTTT GCAAAGTTAT TCGCAAGTAA AAGTCTGATT
    TCAGAGGTGG TGAAGGTGGT TCAGCAGACG CTGCTGCCAT CTCTGAATCC AAACCCAACT
    GGTGTGGAAG CTCTGAGACT TTATCTTCTC CTCCCTGAGC TCATCAATGG TCTCAAGAAA
    CAAAGAACCA AACTCATTGA AGCTCTGGCC TCCAAAATCC TTGAGCTGAA TTCTGCAGCT
    CGCAAGGTGC TAGAGATGTA TTGGTCCAGA CTCCCTGATG ATTGGTTTGA ACGCTTAATA
    AAGTTATTTC ACAAAGAATC CACTGATCTG ATTGACCAGA TTTGTGATGA AACTGACCAT
    CAGTCAACCA GACACCTGAA GAACATTTTG GAGATCCTTC AGATGGCCTA TCAGATCAGT
    TGCAGTACCG ACATTACGCA TCATGATTTC ATCATTCATA AGATCAACGA TCTCCTAGAC
    CTACTGCAAA CCATCACGGC AGACCTGCTC AGAATGGAAT TATACATGCA AATCAACGAA
    GACTTCCATG ATATGGTGAA ACTGGAAGAG TACTACTTTA GAACAGTTAA AATATTGGTC
    AAGTTCCCTT TTGCGTCTGA CTTTATAACT AAACAGAGAA TGTTTAATCT CTTGCAGGAG
    GAATGTTTTC GGAACGTACC AAACCTGGCA ATGGGAAATA GTTTTAGCAA CTTGTTGCAC
    ATAAACAGGG AATCAGTGCT GACGGACACC CTTCAGTATC TTAGACAAAA CACTCACTCG
    TTTATGCATC CATTGCAGGT GGCGTTCATC GGGGAGGATG GAATAGATAT GAGGGGTTTA
    TCAGCAGAAT TCTTCTCCCT TATCTCCAAG TCTTTTATTG AATGGGATAA AAAGATTCTG
    GAAGTTCATG AGAGTTCACT GGTTTGGTTC AATCCTCATC ATACACGGGG CAATAAGAAC
    TTCTACTATC TTGGAGTGAT CTGTGGAATG GCGCTCTACA ACAGACATCA CATCAACATT
    GACTTTCCAC TGGCCTTATT CAAGAAACTC CTCCAGCAGA AGCCCAGTCT GGATGATCTG
    GAGGAGCTCT CACCTGTGGA GGCCAGAAGT CTGAAAAACC TTCTTGAGGA GGACGAGGAG
    GAAGTAGTGA ATTTGCAACA TTTGGATTTC ACGTGCAAAG GGCAAGAACT CGTCCCAAAT
    GGAAGTCAAA TTCAAGTAAA CAAAGTTAAC AGACAGAAGT ATGTGGATTT GTATGTTGAT
    CTTGAGTTCA ACAAGTCTGT GAAGACTCAG TTTGAGCAGT TTACAAGAGG TTTCTCTAAG
    GGCTGCCCGC TCGATGCGTG GACCATGTTT CACCCTGAAG AGCTGCAGGA GCTTCTCCAT
    GGCTCTCCTC AATATAACTG GAAGGAGCTA CAACAGTCGG CTTCCTATGA AGGCTGCTCT
    GCCTCTGATG AGCTCATCAA GAACTTCTGG ACTGTTTTCT TTGAGCTCTC TGAAGAGAAC
    AAGAAGAAAT TTCTGATGTT CTTGTATGGA ACGGAGCGTG TACCTGCTGG AGGTTTGTCA
    AAACGTGCTC TGAAGATTTC TCAGACAGAC TGTCCTGATC CAGATGACCA TTTGCCAGAA
    GCCCAGACCT GTTTTGAAAG ATTAGTTCTT CCAAAATACA GTGATATCAA CACACTTCAC
    GATAAGTTAA TACATGCAAT CAACTCTTGT GCTGTTTTTG GACGAGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211160.1
    CDS171..2861
    Translation

    Target ORF information:

    RefSeq Version XM_017355671.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-281g13.5 (si:ch211-281g13.5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355671.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCAAT CTACAGTGTG CAGACCTCTT AAAAGTCTGG AGAACAGGCA GGTGATTCAG 
    ATCGCATGTG GAGACCAGCA TTCAATGGCG CTGAGCAATG ATGGTCAGTT GTTCGTTTGG
    GGCGAGAACA CTCATGGTCA GCTGGGTTTG AGGAAAGAGC AGGCCGGCAC TCAGTCTCCT
    CAACACCTCC AGAGTCTGTG TGAGATCCCA GTGGCTCAGA TCAGCGCTGG AGGAAACCAC
    AGCTTTGTGT TGTCTCTGTC AGGAGTCGTG TTTGGGTGGG GGAGCAACTC TGCTGGACAG
    CTGGGTCTCG GAGACACTAA AGACAGATTC GTCCCTACAA TTGTTAAAAG CTTGTGTGGA
    AAGAAAACAG TGTCCATCTC ATGTGGAGGA GAACACACTG CCACTCTGTC AAAGGGAGGA
    ACAGTGTTCA CATTTGGATC AGGAGGTTTT GGTCAGCTTG GGCACAACTC ACTTAAAGAT
    GAACATCATC CACGACTGAT TGCTGAACTG TGGGGATCTA AAGTGTCGCA GGTCACATGT
    GGGAGACATC ACACGCTTGT ATTTGAGGAA TCGTCAAAGC TGATCTACTC ATTTGGATGT
    GGGATGCAAG GGCAGCTGGG AAATGGAGAA CAGGTCAAGC AGTCTGTGCC GTTACCAGTG
    CTTCTGCCAA CTGAATGTAC TGAATTTACG ATGGAAAAAC TCATCACTGG AGAACACCAC
    TCCTTTGCCT TGTTTTTCAA GAATCCTGGA AATGAGTCTG AAAAGCCTAA ATCTGGTTCA
    AATGGAGGAA TTTTGACACT AAATGACAGA ATGATTGACC GCTGGGTGTC TGAAAGTGAC
    CCATGGCCAA CAGTAAAGAA TGAAATAAAC ACAGTGTTCT CCTCCGCTGC CTCACTGAAT
    GGAAGTTTTC TCAAAACAAG TCGCGATGAA CATTATCAGA CGTCTGTGGA GCATTGTGGC
    CTGGATTTTG ATCTGGTTAA AACATCCTTT GCAAAGTTAT TCGCAAGTAA AAGTCTGATT
    TCAGAGGTGG TGAAGGTGGT TCAGCAGACG CTGCTGCCAT CTCTGAATCC AAACCCAACT
    GGTGTGGAAG CTCTGAGACT TTATCTTCTC CTCCCTGAGC TCATCAATGG TCTCAAGAAA
    CAAAGAACCA AACTCATTGA AGCTCTGGCC TCCAAAATCC TTGAGCTGAA TTCTGCAGCT
    CGCAAGGTGC TAGAGATGTA TTGGTCCAGA CTCCCTGATG ATTGGTTTGA ACGCTTAATA
    AAGTTATTTC ACAAAGAATC CACTGATCTG ATTGACCAGA TTTGTGATGA AACTGACCAT
    CAGTCAACCA GACACCTGAA GAACATTTTG GAGATCCTTC AGATGGCCTA TCAGATCAGT
    TGCAGTACCG ACATTACGCA TCATGATTTC ATCATTCATA AGATCAACGA TCTCCTAGAC
    CTACTGCAAA CCATCACGGC AGACCTGCTC AGAATGGAAT TATACATGCA AATCAACGAA
    GACTTCCATG ATATGGTGAA ACTGGAAGAG TACTACTTTA GAACAGTTAA AATATTGGTC
    AAGTTCCCTT TTGCGTCTGA CTTTATAACT AAACAGAGAA TGTTTAATCT CTTGCAGGAG
    GAATGTTTTC GGAACGTACC AAACCTGGCA ATGGGAAATA GTTTTAGCAA CTTGTTGCAC
    ATAAACAGGG AATCAGTGCT GACGGACACC CTTCAGTATC TTAGACAAAA CACTCACTCG
    TTTATGCATC CATTGCAGGT GGCGTTCATC GGGGAGGATG GAATAGATAT GAGGGGTTTA
    TCAGCAGAAT TCTTCTCCCT TATCTCCAAG TCTTTTATTG AATGGGATAA AAAGATTCTG
    GAAGTTCATG AGAGTTCACT GGTTTGGTTC AATCCTCATC ATACACGGGG CAATAAGAAC
    TTCTACTATC TTGGAGTGAT CTGTGGAATG GCGCTCTACA ACAGACATCA CATCAACATT
    GACTTTCCAC TGGCCTTATT CAAGAAACTC CTCCAGCAGA AGCCCAGTCT GGATGATCTG
    GAGGAGCTCT CACCTGTGGA GGCCAGAAGT CTGAAAAACC TTCTTGAGGA GGACGAGGAG
    GAAGTAGTGA ATTTGCAACA TTTGGATTTC ACGTGCAAAG GGCAAGAACT CGTCCCAAAT
    GGAAGTCAAA TTCAAGTAAA CAAAGTTAAC AGACAGAAGT ATGTGGATTT GTATGTTGAT
    CTTGAGTTCA ACAAGTCTGT GAAGACTCAG TTTGAGCAGT TTACAAGAGG TTTCTCTAAG
    GGCTGCCCGC TCGATGCGTG GACCATGTTT CACCCTGAAG AGCTGCAGGA GCTTCTCCAT
    GGCTCTCCTC AATATAACTG GAAGGAGCTA CAACAGTCGG CTTCCTATGA AGGCTGCTCT
    GCCTCTGATG AGCTCATCAA GAACTTCTGG ACTGTTTTCT TTGAGCTCTC TGAAGAGAAC
    AAGAAGAAAT TTCTGATGTT CTTGTATGGA ACGGAGCGTG TACCTGCTGG AGGTTTGTCA
    AAACGTGCTC TGAAGATTTC TCAGACAGAC TGTCCTGATC CAGATGACCA TTTGCCAGAA
    GCCCAGACCT GTTTTGAAAG ATTAGTTCTT CCAAAATACA GTGATATCAA CACACTTCAC
    GATAAGTTAA TACATGCAAT CAACTCTTGT GCTGTTTTTG GACGAGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53658
    Clone ID Related Accession (Same CDS sequence) XM_017355671.1 , XM_021475845.1
    Accession Version XM_021475845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product probable E3 ubiquitin-protein ligase HERC4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCAAT CTACAGTGTG CAGACCTCTT AAAAGTCTGG AGAACAGGCA GGTGATTCAG 
    ATCGCATGTG GAGACCAGCA TTCAATGGCG CTGAGCAATG ATGGTCAGTT GTTCGTTTGG
    GGCGAGAACA CTCATGGTCA GCTGGGTTTG AGGAAAGAGC AGGCCGGCAC TCAGTCTCCT
    CAACACCTCC AGAGTCTGTG TGAGATCCCA GTGGCTCAGA TCAGCGCTGG AGGAAACCAC
    AGCTTTGTGT TGTCTCTGTC AGGAGTCGTG TTTGGGTGGG GGAGCAACTC TGCTGGACAG
    CTGGGTCTCG GAGACACTAA AGACAGATTC GTCCCTACAA TTGTTAAAAG CTTGTGTGGA
    AAGAAAACAG TGTCCATCTC ATGTGGAGGA GAACACACTG CCACTCTGTC AAAGGGAGGA
    ACAGTGTTCA CATTTGGATC AGGAGGTTTT GGTCAGCTTG GGCACAACTC ACTTAAAGAT
    GAACATCATC CACGACTGAT TGCTGAACTG TGGGGATCTA AAGTGTCGCA GGTCACATGT
    GGGAGACATC ACACGCTTGT ATTTGAGGAA TCGTCAAAGC TGATCTACTC ATTTGGATGT
    GGGATGCAAG GGCAGCTGGG AAATGGAGAA CAGGTCAAGC AGTCTGTGCC GTTACCAGTG
    CTTCTGCCAA CTGAATGTAC TGAATTTACG ATGGAAAAAC TCATCACTGG AGAACACCAC
    TCCTTTGCCT TGTTTTTCAA GAATCCTGGA AATGAGTCTG AAAAGCCTAA ATCTGGTTCA
    AATGGAGGAA TTTTGACACT AAATGACAGA ATGATTGACC GCTGGGTGTC TGAAAGTGAC
    CCATGGCCAA CAGTAAAGAA TGAAATAAAC ACAGTGTTCT CCTCCGCTGC CTCACTGAAT
    GGAAGTTTTC TCAAAACAAG TCGCGATGAA CATTATCAGA CGTCTGTGGA GCATTGTGGC
    CTGGATTTTG ATCTGGTTAA AACATCCTTT GCAAAGTTAT TCGCAAGTAA AAGTCTGATT
    TCAGAGGTGG TGAAGGTGGT TCAGCAGACG CTGCTGCCAT CTCTGAATCC AAACCCAACT
    GGTGTGGAAG CTCTGAGACT TTATCTTCTC CTCCCTGAGC TCATCAATGG TCTCAAGAAA
    CAAAGAACCA AACTCATTGA AGCTCTGGCC TCCAAAATCC TTGAGCTGAA TTCTGCAGCT
    CGCAAGGTGC TAGAGATGTA TTGGTCCAGA CTCCCTGATG ATTGGTTTGA ACGCTTAATA
    AAGTTATTTC ACAAAGAATC CACTGATCTG ATTGACCAGA TTTGTGATGA AACTGACCAT
    CAGTCAACCA GACACCTGAA GAACATTTTG GAGATCCTTC AGATGGCCTA TCAGATCAGT
    TGCAGTACCG ACATTACGCA TCATGATTTC ATCATTCATA AGATCAACGA TCTCCTAGAC
    CTACTGCAAA CCATCACGGC AGACCTGCTC AGAATGGAAT TATACATGCA AATCAACGAA
    GACTTCCATG ATATGGTGAA ACTGGAAGAG TACTACTTTA GAACAGTTAA AATATTGGTC
    AAGTTCCCTT TTGCGTCTGA CTTTATAACT AAACAGAGAA TGTTTAATCT CTTGCAGGAG
    GAATGTTTTC GGAACGTACC AAACCTGGCA ATGGGAAATA GTTTTAGCAA CTTGTTGCAC
    ATAAACAGGG AATCAGTGCT GACGGACACC CTTCAGTATC TTAGACAAAA CACTCACTCG
    TTTATGCATC CATTGCAGGT GGCGTTCATC GGGGAGGATG GAATAGATAT GAGGGGTTTA
    TCAGCAGAAT TCTTCTCCCT TATCTCCAAG TCTTTTATTG AATGGGATAA AAAGATTCTG
    GAAGTTCATG AGAGTTCACT GGTTTGGTTC AATCCTCATC ATACACGGGG CAATAAGAAC
    TTCTACTATC TTGGAGTGAT CTGTGGAATG GCGCTCTACA ACAGACATCA CATCAACATT
    GACTTTCCAC TGGCCTTATT CAAGAAACTC CTCCAGCAGA AGCCCAGTCT GGATGATCTG
    GAGGAGCTCT CACCTGTGGA GGCCAGAAGT CTGAAAAACC TTCTTGAGGA GGACGAGGAG
    GAAGTAGTGA ATTTGCAACA TTTGGATTTC ACGTGCAAAG GGCAAGAACT CGTCCCAAAT
    GGAAGTCAAA TTCAAGTAAA CAAAGTTAAC AGACAGAAGT ATGTGGATTT GTATGTTGAT
    CTTGAGTTCA ACAAGTCTGT GAAGACTCAG TTTGAGCAGT TTACAAGAGG TTTCTCTAAG
    GGCTGCCCGC TCGATGCGTG GACCATGTTT CACCCTGAAG AGCTGCAGGA GCTTCTCCAT
    GGCTCTCCTC AATATAACTG GAAGGAGCTA CAACAGTCGG CTTCCTATGA AGGCTGCTCT
    GCCTCTGATG AGCTCATCAA GAACTTCTGG ACTGTTTTCT TTGAGCTCTC TGAAGAGAAC
    AAGAAGAAAT TTCTGATGTT CTTGTATGGA ACGGAGCGTG TACCTGCTGG AGGTTTGTCA
    AAACGTGCTC TGAAGATTTC TCAGACAGAC TGTCCTGATC CAGATGACCA TTTGCCAGAA
    GCCCAGACCT GTTTTGAAAG ATTAGTTCTT CCAAAATACA GTGATATCAA CACACTTCAC
    GATAAGTTAA TACATGCAAT CAACTCTTGT GCTGTTTTTG GACGAGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331520.1
    CDS545..3235
    Translation

    Target ORF information:

    RefSeq Version XM_021475845.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-281g13.5 (si:ch211-281g13.5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCAAT CTACAGTGTG CAGACCTCTT AAAAGTCTGG AGAACAGGCA GGTGATTCAG 
    ATCGCATGTG GAGACCAGCA TTCAATGGCG CTGAGCAATG ATGGTCAGTT GTTCGTTTGG
    GGCGAGAACA CTCATGGTCA GCTGGGTTTG AGGAAAGAGC AGGCCGGCAC TCAGTCTCCT
    CAACACCTCC AGAGTCTGTG TGAGATCCCA GTGGCTCAGA TCAGCGCTGG AGGAAACCAC
    AGCTTTGTGT TGTCTCTGTC AGGAGTCGTG TTTGGGTGGG GGAGCAACTC TGCTGGACAG
    CTGGGTCTCG GAGACACTAA AGACAGATTC GTCCCTACAA TTGTTAAAAG CTTGTGTGGA
    AAGAAAACAG TGTCCATCTC ATGTGGAGGA GAACACACTG CCACTCTGTC AAAGGGAGGA
    ACAGTGTTCA CATTTGGATC AGGAGGTTTT GGTCAGCTTG GGCACAACTC ACTTAAAGAT
    GAACATCATC CACGACTGAT TGCTGAACTG TGGGGATCTA AAGTGTCGCA GGTCACATGT
    GGGAGACATC ACACGCTTGT ATTTGAGGAA TCGTCAAAGC TGATCTACTC ATTTGGATGT
    GGGATGCAAG GGCAGCTGGG AAATGGAGAA CAGGTCAAGC AGTCTGTGCC GTTACCAGTG
    CTTCTGCCAA CTGAATGTAC TGAATTTACG ATGGAAAAAC TCATCACTGG AGAACACCAC
    TCCTTTGCCT TGTTTTTCAA GAATCCTGGA AATGAGTCTG AAAAGCCTAA ATCTGGTTCA
    AATGGAGGAA TTTTGACACT AAATGACAGA ATGATTGACC GCTGGGTGTC TGAAAGTGAC
    CCATGGCCAA CAGTAAAGAA TGAAATAAAC ACAGTGTTCT CCTCCGCTGC CTCACTGAAT
    GGAAGTTTTC TCAAAACAAG TCGCGATGAA CATTATCAGA CGTCTGTGGA GCATTGTGGC
    CTGGATTTTG ATCTGGTTAA AACATCCTTT GCAAAGTTAT TCGCAAGTAA AAGTCTGATT
    TCAGAGGTGG TGAAGGTGGT TCAGCAGACG CTGCTGCCAT CTCTGAATCC AAACCCAACT
    GGTGTGGAAG CTCTGAGACT TTATCTTCTC CTCCCTGAGC TCATCAATGG TCTCAAGAAA
    CAAAGAACCA AACTCATTGA AGCTCTGGCC TCCAAAATCC TTGAGCTGAA TTCTGCAGCT
    CGCAAGGTGC TAGAGATGTA TTGGTCCAGA CTCCCTGATG ATTGGTTTGA ACGCTTAATA
    AAGTTATTTC ACAAAGAATC CACTGATCTG ATTGACCAGA TTTGTGATGA AACTGACCAT
    CAGTCAACCA GACACCTGAA GAACATTTTG GAGATCCTTC AGATGGCCTA TCAGATCAGT
    TGCAGTACCG ACATTACGCA TCATGATTTC ATCATTCATA AGATCAACGA TCTCCTAGAC
    CTACTGCAAA CCATCACGGC AGACCTGCTC AGAATGGAAT TATACATGCA AATCAACGAA
    GACTTCCATG ATATGGTGAA ACTGGAAGAG TACTACTTTA GAACAGTTAA AATATTGGTC
    AAGTTCCCTT TTGCGTCTGA CTTTATAACT AAACAGAGAA TGTTTAATCT CTTGCAGGAG
    GAATGTTTTC GGAACGTACC AAACCTGGCA ATGGGAAATA GTTTTAGCAA CTTGTTGCAC
    ATAAACAGGG AATCAGTGCT GACGGACACC CTTCAGTATC TTAGACAAAA CACTCACTCG
    TTTATGCATC CATTGCAGGT GGCGTTCATC GGGGAGGATG GAATAGATAT GAGGGGTTTA
    TCAGCAGAAT TCTTCTCCCT TATCTCCAAG TCTTTTATTG AATGGGATAA AAAGATTCTG
    GAAGTTCATG AGAGTTCACT GGTTTGGTTC AATCCTCATC ATACACGGGG CAATAAGAAC
    TTCTACTATC TTGGAGTGAT CTGTGGAATG GCGCTCTACA ACAGACATCA CATCAACATT
    GACTTTCCAC TGGCCTTATT CAAGAAACTC CTCCAGCAGA AGCCCAGTCT GGATGATCTG
    GAGGAGCTCT CACCTGTGGA GGCCAGAAGT CTGAAAAACC TTCTTGAGGA GGACGAGGAG
    GAAGTAGTGA ATTTGCAACA TTTGGATTTC ACGTGCAAAG GGCAAGAACT CGTCCCAAAT
    GGAAGTCAAA TTCAAGTAAA CAAAGTTAAC AGACAGAAGT ATGTGGATTT GTATGTTGAT
    CTTGAGTTCA ACAAGTCTGT GAAGACTCAG TTTGAGCAGT TTACAAGAGG TTTCTCTAAG
    GGCTGCCCGC TCGATGCGTG GACCATGTTT CACCCTGAAG AGCTGCAGGA GCTTCTCCAT
    GGCTCTCCTC AATATAACTG GAAGGAGCTA CAACAGTCGG CTTCCTATGA AGGCTGCTCT
    GCCTCTGATG AGCTCATCAA GAACTTCTGG ACTGTTTTCT TTGAGCTCTC TGAAGAGAAC
    AAGAAGAAAT TTCTGATGTT CTTGTATGGA ACGGAGCGTG TACCTGCTGG AGGTTTGTCA
    AAACGTGCTC TGAAGATTTC TCAGACAGAC TGTCCTGATC CAGATGACCA TTTGCCAGAA
    GCCCAGACCT GTTTTGAAAG ATTAGTTCTT CCAAAATACA GTGATATCAA CACACTTCAC
    GATAAGTTAA TACATGCAAT CAACTCTTGT GCTGTTTTTG GACGAGGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.