ehmt1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ehmt1a
Entrez Gene ID	100005761
Full Name	euchromatic histone-lysine N-methyltransferase 1a
Synonyms	bZ1O1.6,si:rp71-1o1.6,zgc:136244
General protein information	Preferred Names euchromatic histone-lysine N-methyltransferase 1a Names histone-lysine N-methyltransferase, H3 lysine-9 specific 5 euchromatic histone methyltransferase 1a histone methyltransferase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475966.1	XP_021331641.1	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X3
XM_005171778.3	XP_005171835.1	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X1
NM_001030131.2	NP_001025302.1	histone-lysine N-methyltransferase, H3 lysine-9 specific 5
XM_005171779.3	XP_005171836.1	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X2
XM_005171778.4	XP_005171835.1	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X1
XM_005171779.4	XP_005171836.1	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Histological and transcriptomic effects of 17?-methyltestosterone on zebrafish gonad development.
Lee SLJ, Horsfield JA, Black MA, Rutherford K, Fisher A, Gemmell NJ
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RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Genome-wide survey and developmental expression mapping of zebrafish SET domain-containing genes.
Sun XJ, Xu PF, Zhou T, Hu M, Fu CT, Zhang Y, Jin Y, Chen Y, Chen SJ, Huang QH, Liu TX, Chen Z
PloS one3(1)e1499(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ehmt1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ehmt1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10409	XM_021475966.1 Latest version!	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa10408	NM_001030131.2 Latest version!	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10406	XM_005171778.4 Latest version!	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10407	XM_005171779.4 Latest version!	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa10406	XM_005171778.3	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10407	XM_005171779.3	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ehmt1a ( XM_021475966.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331641.1 Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10409
Clone ID Related Accession (Same CDS sequence)	XM_021475966.1
Accession Version	XM_021475966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
GAAGCTTTAC GTATGACCGG GTCCAAGGGT GGACAGATGT CTATTGAGGA GGCATTCAGA 
AATAAAGCTA GTTCTGAGAA ACCAGAGAAA CAGACTTCAA CTGCTGTGAT AGAGGATGCA 
GAACCGCACC AGAACAAAGA AACTGAGGAT GCTCCAGAAG GCCCTGCTGA GGAATACACA 
GAGCTGCCTT TACATGCTCT GGCTCATGAT AGCTTGTTCA CAACAATCCC CACAGGCTTA 
CGGGAGGATA AAGAGAACAT GGAAACTGAT GACACGTTAG AGCCTCCACT GTGTAGCTGC 
CGCATGGAGA CGCCAAAGAA CCAGGATGTA GTCACACTGG CTGAGGGCAA GTGCATGGCT 
GTGGAGAGTG TTGATGGGAA GCTGAGTTTA TGTCGGAAAG TGATCATGAA GCAGGAATTG 
ATGCGTCCAT CTCTGAGGAT TCCACAGCTC GTACTGTGTG AGGATCATCG TGCAGGAATG 
GTGAAACACC AGAGCTGTCC GGGCTGTGGC TTCTTCTGCA GAGCTGGGAC ATTTATGGAG 
TGTCAGCCAG ATGGGAACAT CTCTCACCGC TTCCACCGGA GCTGTGCATC TCTGATCAGA 
GGCCAGGTCT ACTGCCCTCA CTGCGGTGAG GAGGCTAGTC ATGCTAAGGA GGTCACGATC 
CCCAAACCTG ACTGTGTGGC TTCAGTGCCA ACAGCTGCTG TGCCGGCACA CAAGAGAGAC 
AACGCCTTAA TGGACAGGGT TAAATTGGAC TCTGTCATGG TTGATGGAGC AGGAGATCTT 
GCTAAAGAAT CCCTGGAAAG TGTTTTAAAT GCATTGGAGG ATGGCAAGTA TAAGAAATTC 
AAGTTGCCCC CAAAGCAGCT TTATTTCTCT GCTAAGAGAG GAGAGCTGCA AAGAATCTTG 
CATATGCTGG TGGAAGGTGT GGACCCAAAC TTGCGGATGG ACAGCGAGAA GAGGAAGACT 
CCACTACATT GTGCTGCTGA GGAAGGCCAT AAAGACATCT GCCATGTGCT GGTGCAGGCT 
GGTGCGAACC TAGACATGTG TGACATTGAG CAGCGAACGC CTTTGATGTA CGCCTGTAAC 
AACAATCATC TAGAAAATGT AAAATACTTG CTCAAAGCTG GTGCTTCTTC TGCACATAAG 
GATATGAGAG GCTCTACATG TCTTCATTTG GCAGCCAGAG CAGGACATAC CAATGTGCTG 
CAGTATCTGC TCTCCCTGCC ATCCATAGAT GTCAACTGTA AGGATGATGG CGGATGGGCT 
CCTCTCACCT GGGCCACAGA GAACATGCGT CTGGAGCAGG TGAAGATGCT GATTTCAGCA 
GGAGCTGATG TCCAAATAAG AGATAAGGAG GAGAATTTGT GCCTCCACTG GGCTGCTTTC 
TCTGGCTGTG ATGAAATCGC TCAGCTGCTG CTGGACCACA GATCTGACCT GCACGCGGTG 
AACGTCCATG GAGACACTCC TCTTCACATC GCTGTCAGAC AGAACCAACT GGACTGCGTC 
ATGTTGTTTC TGTCTCGAGG AGCGGATGTA AACCTGAAGA ACAGAGATGG CGAAACTCCT 
TTGGGCTGCT GTAATATAAA CTCCAAAATG TGGACAATCC TGAACACCAA TAAGAAGCTG 
ACTGATGCGA GGAGAGGACG AGAAAGCCTG AGAGAGAGAC TCCTCTGCAG AGATGTGTCC 
CGAGGCTATG AGGACATTCC TGTGCCCTGT GTGAATGGGG TGGACCACGA GCCCTGCCCC 
AGCAACTTCA AATACATTCC GGAGAACTGC TTTACCTCCC AAGTAAATAT AGATGAGAAC 
ATAAAGCACT TACAGCACTG CAGTTGTAAA GATGACTGTG CGTCTAGCAG CTGTATCTGT 
GGCCAGCTCA GCATGCACTG CTGGTATGGC AAGGATGGCC GATTGCTAAA AGAATTCTGC 
CGGGATGATC CACCCTTTCT GTTCGAGTGT AACCACGCCT GTTCCTGTTG GAGAACGTGC 
CGGAATAGAG TCATACAGAA TGGCCTTCGG CTCAGGCTGC AGGTGTTTAG GACTGAGAGG 
ATGGGTTGGG GAGTTCGAAC ACTGCAGGAT ATTCCTGAGG GAGGTTTTGT ATGCGAGTTT 
GCTGGTGAAA TAATTTCCGA TGGAGAAGCT AACATTCGGG AAAACGACTC CTACATGTTC 
AACCTAGACA ATAAGGTTGG AGAGGCCTAC TGCATTGATG GACAATTTTA TGGTAATGTT 
AGCAGGTTTA TGAATCATCT TTGTGAGCCC AATCTATTCC CAGTACGAGT GTTCACCAAA 
CATCAAGACA TGCGCTTTCC TCGTATTGCA TTCTTTGCAA GCAAGCATAT TCAAGCTGGA 
GATGAGCTTG GGTTTGACTA TGGGGATCAT TACTGGCAAA TCAAGAAAAA GTACTTCCGC 
TGCCAATGTG GCTCTGGAAA ATGTAGGTAC TCTGAGGTCG TTCCGGACCG GAACCATGCA 
GAAAGCTCAG CCCAAACCCT CAGTAATCAG ACTGTTGCTC AGGTGGAACA CATTCAGTCT 
TCTAACACTG CTATAGCAAT AACTCAACCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331641.1
CDS	173..3145
Translation	MAKTKKGMDSLADPQRKGKRPKSLGASPKASKQLSASGSRKSTGSSFQLLSGQTSLKPPAENIISGNHSSVQQDKPETLEKQSFGSDNADKRIPVVSVPEAAHEEDEQREPEGVKVVKPAPPRLAWKTMVRSAPAPPALKEALRMTGSKGGQMSIEEAFRNKASSEKPEKQTSTAVIEDAEPHQNKETEDAPEGPAEEYTELPLHALAHDSLFTTIPTGLREDKENMETDDTLEPPLCSCRMETPKNQDVVTLAEGKCMAVESVDGKLSLCRKVIMKQELMRPSLRIPQLVLCEDHRAGMVKHQSCPGCGFFCRAGTFMECQPDGNISHRFHRSCASLIRGQVYCPHCGEEASHAKEVTIPKPDCVASVPTAAVPAHKRDNALMDRVKLDSVMVDGAGDLAKESLESVLNALEDGKYKKFKLPPKQLYFSAKRGELQRILHMLVEGVDPNLRMDSEKRKTPLHCAAEEGHKDICHVLVQAGANLDMCDIEQRTPLMYACNNNHLENVKYLLKAGASSAHKDMRGSTCLHLAARAGHTNVLQYLLSLPSIDVNCKDDGGWAPLTWATENMRLEQVKMLISAGADVQIRDKEENLCLHWAAFSGCDEIAQLLLDHRSDLHAVNVHGDTPLHIAVRQNQLDCVMLFLSRGADVNLKNRDGETPLGCCNINSKMWTILNTNKKLTDARRGRESLRERLLCRDVSRGYEDIPVPCVNGVDHEPCPSNFKYIPENCFTSQVNIDENIKHLQHCSCKDDCASSSCICGQLSMHCWYGKDGRLLKEFCRDDPPFLFECNHACSCWRTCRNRVIQNGLRLRLQVFRTERMGWGVRTLQDIPEGGFVCEFAGEIISDGEANIRENDSYMFNLDNKVGEAYCIDGQFYGNVSRFMNHLCEPNLFPVRVFTKHQDMRFPRIAFFASKHIQAGDELGFDYGDHYWQIKKKYFRCQCGSGKCRYSEVVPDRNHAESSAQTLSNQTVAQVEHIQSSNTAIAITQP

Target ORF information:

RefSeq Version	XM_021475966.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475966.1

ORF Insert Sequence:

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
GAAGCTTTAC GTATGACCGG GTCCAAGGGT GGACAGATGT CTATTGAGGA GGCATTCAGA 
AATAAAGCTA GTTCTGAGAA ACCAGAGAAA CAGACTTCAA CTGCTGTGAT AGAGGATGCA 
GAACCGCACC AGAACAAAGA AACTGAGGAT GCTCCAGAAG GCCCTGCTGA GGAATACACA 
GAGCTGCCTT TACATGCTCT GGCTCATGAT AGCTTGTTCA CAACAATCCC CACAGGCTTA 
CGGGAGGATA AAGAGAACAT GGAAACTGAT GACACGTTAG AGCCTCCACT GTGTAGCTGC 
CGCATGGAGA CGCCAAAGAA CCAGGATGTA GTCACACTGG CTGAGGGCAA GTGCATGGCT 
GTGGAGAGTG TTGATGGGAA GCTGAGTTTA TGTCGGAAAG TGATCATGAA GCAGGAATTG 
ATGCGTCCAT CTCTGAGGAT TCCACAGCTC GTACTGTGTG AGGATCATCG TGCAGGAATG 
GTGAAACACC AGAGCTGTCC GGGCTGTGGC TTCTTCTGCA GAGCTGGGAC ATTTATGGAG 
TGTCAGCCAG ATGGGAACAT CTCTCACCGC TTCCACCGGA GCTGTGCATC TCTGATCAGA 
GGCCAGGTCT ACTGCCCTCA CTGCGGTGAG GAGGCTAGTC ATGCTAAGGA GGTCACGATC 
CCCAAACCTG ACTGTGTGGC TTCAGTGCCA ACAGCTGCTG TGCCGGCACA CAAGAGAGAC 
AACGCCTTAA TGGACAGGGT TAAATTGGAC TCTGTCATGG TTGATGGAGC AGGAGATCTT 
GCTAAAGAAT CCCTGGAAAG TGTTTTAAAT GCATTGGAGG ATGGCAAGTA TAAGAAATTC 
AAGTTGCCCC CAAAGCAGCT TTATTTCTCT GCTAAGAGAG GAGAGCTGCA AAGAATCTTG 
CATATGCTGG TGGAAGGTGT GGACCCAAAC TTGCGGATGG ACAGCGAGAA GAGGAAGACT 
CCACTACATT GTGCTGCTGA GGAAGGCCAT AAAGACATCT GCCATGTGCT GGTGCAGGCT 
GGTGCGAACC TAGACATGTG TGACATTGAG CAGCGAACGC CTTTGATGTA CGCCTGTAAC 
AACAATCATC TAGAAAATGT AAAATACTTG CTCAAAGCTG GTGCTTCTTC TGCACATAAG 
GATATGAGAG GCTCTACATG TCTTCATTTG GCAGCCAGAG CAGGACATAC CAATGTGCTG 
CAGTATCTGC TCTCCCTGCC ATCCATAGAT GTCAACTGTA AGGATGATGG CGGATGGGCT 
CCTCTCACCT GGGCCACAGA GAACATGCGT CTGGAGCAGG TGAAGATGCT GATTTCAGCA 
GGAGCTGATG TCCAAATAAG AGATAAGGAG GAGAATTTGT GCCTCCACTG GGCTGCTTTC 
TCTGGCTGTG ATGAAATCGC TCAGCTGCTG CTGGACCACA GATCTGACCT GCACGCGGTG 
AACGTCCATG GAGACACTCC TCTTCACATC GCTGTCAGAC AGAACCAACT GGACTGCGTC 
ATGTTGTTTC TGTCTCGAGG AGCGGATGTA AACCTGAAGA ACAGAGATGG CGAAACTCCT 
TTGGGCTGCT GTAATATAAA CTCCAAAATG TGGACAATCC TGAACACCAA TAAGAAGCTG 
ACTGATGCGA GGAGAGGACG AGAAAGCCTG AGAGAGAGAC TCCTCTGCAG AGATGTGTCC 
CGAGGCTATG AGGACATTCC TGTGCCCTGT GTGAATGGGG TGGACCACGA GCCCTGCCCC 
AGCAACTTCA AATACATTCC GGAGAACTGC TTTACCTCCC AAGTAAATAT AGATGAGAAC 
ATAAAGCACT TACAGCACTG CAGTTGTAAA GATGACTGTG CGTCTAGCAG CTGTATCTGT 
GGCCAGCTCA GCATGCACTG CTGGTATGGC AAGGATGGCC GATTGCTAAA AGAATTCTGC 
CGGGATGATC CACCCTTTCT GTTCGAGTGT AACCACGCCT GTTCCTGTTG GAGAACGTGC 
CGGAATAGAG TCATACAGAA TGGCCTTCGG CTCAGGCTGC AGGTGTTTAG GACTGAGAGG 
ATGGGTTGGG GAGTTCGAAC ACTGCAGGAT ATTCCTGAGG GAGGTTTTGT ATGCGAGTTT 
GCTGGTGAAA TAATTTCCGA TGGAGAAGCT AACATTCGGG AAAACGACTC CTACATGTTC 
AACCTAGACA ATAAGGTTGG AGAGGCCTAC TGCATTGATG GACAATTTTA TGGTAATGTT 
AGCAGGTTTA TGAATCATCT TTGTGAGCCC AATCTATTCC CAGTACGAGT GTTCACCAAA 
CATCAAGACA TGCGCTTTCC TCGTATTGCA TTCTTTGCAA GCAAGCATAT TCAAGCTGGA 
GATGAGCTTG GGTTTGACTA TGGGGATCAT TACTGGCAAA TCAAGAAAAA GTACTTCCGC 
TGCCAATGTG GCTCTGGAAA ATGTAGGTAC TCTGAGGTCG TTCCGGACCG GAACCATGCA 
GAAAGCTCAG CCCAAACCCT CAGTAATCAG ACTGTTGCTC AGGTGGAACA CATTCAGTCT 
TCTAACACTG CTATAGCAAT AACTCAACCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1a ( NM_001030131.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025302.1 Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), mRNA.

Price & Availability↑

CloneID	ODa10408
Clone ID Related Accession (Same CDS sequence)	NM_001030131.2
Accession Version	NM_001030131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-9 specific 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL831768.9 and AL929342.7. On May 27, 2010 this sequence version replaced NM_001030131.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG ACAGGGTTAA ATTGGACTCT 
GTCATGGTTG ATGGAGCAGG AGATCTTGCT AAAGAATCCC TGGAAAGTGT TTTAAATGCA 
TTGGAGGATG GCAAGTATAA GAAATTCAAG TTGCCCCCAA AGCAGCTTTA TTTCTCTGCT 
AAGAGAGGAG AGCTGCAAAG AATCTTGCAT ATGCTGGTGG AAGGTGTGGA CCCAAACTTG 
CGGATGGACA GCGAGAAGAG GAAGACTCCA CTACATTGTG CTGCTGAGGA AGGCCATAAA 
GACATCTGCC ATGTGCTGGT GCAGGCTGGT GCGAACCTAG ACATGTGTGA CATTGAGCAG 
CGAACGCCTT TGATGTACGC CTGTAACAAC AATCATCTAG AAAATGTAAA ATACTTGCTC 
AAAGCTGGTG CTTCTTCTGC ACATAAGGAT ATGAGAGGCT CTACATGTCT TCATTTGGCA 
GCCAGAGCAG GACATACCAA TGTGCTGCAG TATCTGCTCT CCCTGCCATC CATAGATGTC 
AACTGTAAGG ATGATGGCGG ATGGGCTCCT CTCACCTGGG CCACAGAGAA CATGCGTCTG 
GAGCAGGTGA AGATGCTGAT TTCAGCAGGA GCTGATGTCC AAATAAGAGA TAAGGAGGAG 
AATTTGTGCC TCCACTGGGC TGCTTTCTCT GGCTGTGATG AAATCGCTCA GCTGCTGCTG 
GACCACAGAT CTGACCTGCA CGCGGTGAAC GTCCATGGAG ACACTCCTCT TCACATCGCT 
GTCAGACAGA ACCAACTGGA CTGCGTCATG TTGTTTCTGT CTCGAGGAGC GGATGTAAAC 
CTGAAGAACA GAGATGGCGA AACTCCTTTG GGCTGCTGTA ATATAAACTC CAAAATGTGG 
ACAATCCTGA ACACCAATAA GAAGCTGACT GATGCGAGGA GAGGACGAGA AAGCCTGAGA 
GAGAGACTCC TCTGCAGAGA TGTGTCCCGA GGCTATGAGG ACATTCCTGT GCCCTGTGTG 
AATGGGGTGG ACCACGAGCC CTGCCCCAGC AACTTCAAAT ACATTCCGGA GAACTGCTTT 
ACCTCCCAAG TAAATATAGA TGAGAACATA AAGCACTTAC AGCACTGCAG TTGTAAAGAT 
GACTGTGCGT CTAGCAGCTG TATCTGTGGC CAGCTCAGCA TGCACTGCTG GTATGGCAAG 
GATGGCCGAT TGCTAAAAGA ATTCTGCCGG GATGATCCAC CCTTTCTGTT CGAGTGTAAC 
CACGCCTGTT CCTGTTGGAG AACGTGCCGG AATAGAGTCA TACAGAATGG CCTTCGGCTC 
AGGCTGCAGG TGTTTAGGAC TGAGAGGATG GGTTGGGGAG TTCGAACACT GCAGGATATT 
CCTGAGGGAG GTTTTGTATG CGAGTTTGCT GGTGAAATAA TTTCCGATGG AGAAGCTAAC 
ATTCGGGAAA ACGACTCCTA CATGTTCAAC CTAGACAATA AGGCAAAGGC CTACTGCATT 
GATGGACAAT TTTATGGTAA TGTTAGCAGG TTTATGAATC ATCTTTGTGA GCCCAATCTA 
TTCCCAGTAC GAGTGTTCAC CAAACATCAA GACATGCGCT TTCCTCGTAT TGCATTCTTT 
GCAAGCAAGC ATATTCAAGC TGGAGATGAG CTTGGGTTTG ACTATGGGGA TCATTACTGG 
CAAATCAAGA AAAAGTACTT CCGCTGCCAA TGTGGCTCTG GAAAATGTAG GTACTCTGAG 
GTCGTTCCGG ACCGGAACCA TGCAGAAAGC TCAGCCCAAA CCCTCAGTAA TCAGACTGTT 
GCTCAGGTGG AACACATTCA GTCTTCTAAC ACTGCTATAG CAATAACTCA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025302.1
CDS	1..3177
Translation	MAKTKKGMDSLADPQRKGKRPKSLGASPKASKQLSASGSRKSTGSSFQLLSGQTSLKPPAENIISGNHSSVQQDKPETLEKQSFGSDNADKRIPVVSVPEAAHEEDEQREPEGVKVVKPAPPRLAWKTMVRSAPAPPALKTLNRERSGYERDVESSPTQHLSLPQELKDSTQTAPKKKKRKMGLYNFVPKKKPKGSKKANVSLSSSNLQEALRMTGSKGGQMSIEEAFRNKASSEKPEKQTSTAVIEDAEPHQNKETEDAPEGPAEEYTELPLHALAHDSLFTTIPTGLREDKENMETDDTLEPPLCSCRMETPKNQDVVTLAEGKCMAVESVDGKLSLCRKVIMKQELMRPSLRIPQLVLCEDHRAGMVKHQSCPGCGFFCRAGTFMECQPDGNISHRFHRSCASLIRGQVYCPHCGEEASHAKEVTIPKPDCVASVPTAAVPAHKRDNALMDRVKLDSVMVDGAGDLAKESLESVLNALEDGKYKKFKLPPKQLYFSAKRGELQRILHMLVEGVDPNLRMDSEKRKTPLHCAAEEGHKDICHVLVQAGANLDMCDIEQRTPLMYACNNNHLENVKYLLKAGASSAHKDMRGSTCLHLAARAGHTNVLQYLLSLPSIDVNCKDDGGWAPLTWATENMRLEQVKMLISAGADVQIRDKEENLCLHWAAFSGCDEIAQLLLDHRSDLHAVNVHGDTPLHIAVRQNQLDCVMLFLSRGADVNLKNRDGETPLGCCNINSKMWTILNTNKKLTDARRGRESLRERLLCRDVSRGYEDIPVPCVNGVDHEPCPSNFKYIPENCFTSQVNIDENIKHLQHCSCKDDCASSSCICGQLSMHCWYGKDGRLLKEFCRDDPPFLFECNHACSCWRTCRNRVIQNGLRLRLQVFRTERMGWGVRTLQDIPEGGFVCEFAGEIISDGEANIRENDSYMFNLDNKAKAYCIDGQFYGNVSRFMNHLCEPNLFPVRVFTKHQDMRFPRIAFFASKHIQAGDELGFDYGDHYWQIKKKYFRCQCGSGKCRYSEVVPDRNHAESSAQTLSNQTVAQVEHIQSSNTAIAITQP

Target ORF information:

RefSeq Version	NM_001030131.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030131.2

ORF Insert Sequence:

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG ACAGGGTTAA ATTGGACTCT 
GTCATGGTTG ATGGAGCAGG AGATCTTGCT AAAGAATCCC TGGAAAGTGT TTTAAATGCA 
TTGGAGGATG GCAAGTATAA GAAATTCAAG TTGCCCCCAA AGCAGCTTTA TTTCTCTGCT 
AAGAGAGGAG AGCTGCAAAG AATCTTGCAT ATGCTGGTGG AAGGTGTGGA CCCAAACTTG 
CGGATGGACA GCGAGAAGAG GAAGACTCCA CTACATTGTG CTGCTGAGGA AGGCCATAAA 
GACATCTGCC ATGTGCTGGT GCAGGCTGGT GCGAACCTAG ACATGTGTGA CATTGAGCAG 
CGAACGCCTT TGATGTACGC CTGTAACAAC AATCATCTAG AAAATGTAAA ATACTTGCTC 
AAAGCTGGTG CTTCTTCTGC ACATAAGGAT ATGAGAGGCT CTACATGTCT TCATTTGGCA 
GCCAGAGCAG GACATACCAA TGTGCTGCAG TATCTGCTCT CCCTGCCATC CATAGATGTC 
AACTGTAAGG ATGATGGCGG ATGGGCTCCT CTCACCTGGG CCACAGAGAA CATGCGTCTG 
GAGCAGGTGA AGATGCTGAT TTCAGCAGGA GCTGATGTCC AAATAAGAGA TAAGGAGGAG 
AATTTGTGCC TCCACTGGGC TGCTTTCTCT GGCTGTGATG AAATCGCTCA GCTGCTGCTG 
GACCACAGAT CTGACCTGCA CGCGGTGAAC GTCCATGGAG ACACTCCTCT TCACATCGCT 
GTCAGACAGA ACCAACTGGA CTGCGTCATG TTGTTTCTGT CTCGAGGAGC GGATGTAAAC 
CTGAAGAACA GAGATGGCGA AACTCCTTTG GGCTGCTGTA ATATAAACTC CAAAATGTGG 
ACAATCCTGA ACACCAATAA GAAGCTGACT GATGCGAGGA GAGGACGAGA AAGCCTGAGA 
GAGAGACTCC TCTGCAGAGA TGTGTCCCGA GGCTATGAGG ACATTCCTGT GCCCTGTGTG 
AATGGGGTGG ACCACGAGCC CTGCCCCAGC AACTTCAAAT ACATTCCGGA GAACTGCTTT 
ACCTCCCAAG TAAATATAGA TGAGAACATA AAGCACTTAC AGCACTGCAG TTGTAAAGAT 
GACTGTGCGT CTAGCAGCTG TATCTGTGGC CAGCTCAGCA TGCACTGCTG GTATGGCAAG 
GATGGCCGAT TGCTAAAAGA ATTCTGCCGG GATGATCCAC CCTTTCTGTT CGAGTGTAAC 
CACGCCTGTT CCTGTTGGAG AACGTGCCGG AATAGAGTCA TACAGAATGG CCTTCGGCTC 
AGGCTGCAGG TGTTTAGGAC TGAGAGGATG GGTTGGGGAG TTCGAACACT GCAGGATATT 
CCTGAGGGAG GTTTTGTATG CGAGTTTGCT GGTGAAATAA TTTCCGATGG AGAAGCTAAC 
ATTCGGGAAA ACGACTCCTA CATGTTCAAC CTAGACAATA AGGCAAAGGC CTACTGCATT 
GATGGACAAT TTTATGGTAA TGTTAGCAGG TTTATGAATC ATCTTTGTGA GCCCAATCTA 
TTCCCAGTAC GAGTGTTCAC CAAACATCAA GACATGCGCT TTCCTCGTAT TGCATTCTTT 
GCAAGCAAGC ATATTCAAGC TGGAGATGAG CTTGGGTTTG ACTATGGGGA TCATTACTGG 
CAAATCAAGA AAAAGTACTT CCGCTGCCAA TGTGGCTCTG GAAAATGTAG GTACTCTGAG 
GTCGTTCCGG ACCGGAACCA TGCAGAAAGC TCAGCCCAAA CCCTCAGTAA TCAGACTGTT 
GCTCAGGTGG AACACATTCA GTCTTCTAAC ACTGCTATAG CAATAACTCA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1a ( XM_005171778.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171835.1 Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10406
Clone ID Related Accession (Same CDS sequence)	XM_005171778.3 , XM_005171778.4
Accession Version	XM_005171778.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG ACAGGGTTAA ATTGGACTCT 
GTCATGGTTG ATGGAGCAGG AGATCTTGCT AAAGAATCCC TGGAAAGTGT TTTAAATGCA 
TTGGAGGATG GCAAGTATAA GAAATTCAAG TTGCCCCCAA AGCAGCTTTA TTTCTCTGCT 
AAGAGAGGAG AGCTGCAAAG AATCTTGCAT ATGCTGGTGG AAGGTGTGGA CCCAAACTTG 
CGGATGGACA GCGAGAAGAG GAAGACTCCA CTACATTGTG CTGCTGAGGA AGGCCATAAA 
GACATCTGCC ATGTGCTGGT GCAGGCTGGT GCGAACCTAG ACATGTGTGA CATTGAGCAG 
CGAACGCCTT TGATGTACGC CTGTAACAAC AATCATCTAG AAAATGTAAA ATACTTGCTC 
AAAGCTGGTG CTTCTTCTGC ACATAAGGAT ATGAGAGGCT CTACATGTCT TCATTTGGCA 
GCCAGAGCAG GACATACCAA TGTGCTGCAG TATCTGCTCT CCCTGCCATC CATAGATGTC 
AACTGTAAGG ATGATGGCGG ATGGGCTCCT CTCACCTGGG CCACAGAGAA CATGCGTCTG 
GAGCAGGTGA AGATGCTGAT TTCAGCAGGA GCTGATGTCC AAATAAGAGA TAAGGAGGAG 
AATTTGTGCC TCCACTGGGC TGCTTTCTCT GGCTGTGATG AAATCGCTCA GCTGCTGCTG 
GACCACAGAT CTGACCTGCA CGCGGTGAAC GTCCATGGAG ACACTCCTCT TCACATCGCT 
GTCAGACAGA ACCAACTGGA CTGCGTCATG TTGTTTCTGT CTCGAGGAGC GGATGTAAAC 
CTGAAGAACA GAGATGGCGA AACTCCTTTG GGCTGCTGTA ATATAAACTC CAAAATGTGG 
ACAATCCTGA ACACCAATAA GAAGCTGACT GATGCGAGGA GAGGACGAGA AAGCCTGAGA 
GAGAGACTCC TCTGCAGAGA TGTGTCCCGA GGCTATGAGG ACATTCCTGT GCCCTGTGTG 
AATGGGGTGG ACCACGAGCC CTGCCCCAGC AACTTCAAAT ACATTCCGGA GAACTGCTTT 
ACCTCCCAAG TAAATATAGA TGAGAACATA AAGCACTTAC AGCACTGCAG TTGTAAAGAT 
GACTGTGCGT CTAGCAGCTG TATCTGTGGC CAGCTCAGCA TGCACTGCTG GTATGGCAAG 
GATGGCCGAT TGCTAAAAGA ATTCTGCCGG GATGATCCAC CCTTTCTGTT CGAGTGTAAC 
CACGCCTGTT CCTGTTGGAG AACGTGCCGG AATAGAGTCA TACAGAATGG CCTTCGGCTC 
AGGCTGCAGG TGTTTAGGAC TGAGAGGATG GGTTGGGGAG TTCGAACACT GCAGGATATT 
CCTGAGGGAG GTTTTGTATG CGAGTTTGCT GGTGAAATAA TTTCCGATGG AGAAGCTAAC 
ATTCGGGAAA ACGACTCCTA CATGTTCAAC CTAGACAATA AGGTTGGAGA GGCCTACTGC 
ATTGATGGAC AATTTTATGG TAATGTTAGC AGGTTTATGA ATCATCTTTG TGAGCCCAAT 
CTATTCCCAG TACGAGTGTT CACCAAACAT CAAGACATGC GCTTTCCTCG TATTGCATTC 
TTTGCAAGCA AGCATATTCA AGCTGGAGAT GAGCTTGGGT TTGACTATGG GGATCATTAC 
TGGCAAATCA AGAAAAAGTA CTTCCGCTGC CAATGTGGCT CTGGAAAATG TAGGTACTCT 
GAGGTCGTTC CGGACCGGAA CCATGCAGAA AGCTCAGCCC AAACCCTCAG TAATCAGACT 
GTTGCTCAGG TGGAACACAT TCAGTCTTCT AACACTGCTA TAGCAATAAC TCAACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171835.1
CDS	157..3336
Translation	MAKTKKGMDSLADPQRKGKRPKSLGASPKASKQLSASGSRKSTGSSFQLLSGQTSLKPPAENIISGNHSSVQQDKPETLEKQSFGSDNADKRIPVVSVPEAAHEEDEQREPEGVKVVKPAPPRLAWKTMVRSAPAPPALKTLNRERSGYERDVESSPTQHLSLPQELKDSTQTAPKKKKRKMGLYNFVPKKKPKGSKKANVSLSSSNLQEALRMTGSKGGQMSIEEAFRNKASSEKPEKQTSTAVIEDAEPHQNKETEDAPEGPAEEYTELPLHALAHDSLFTTIPTGLREDKENMETDDTLEPPLCSCRMETPKNQDVVTLAEGKCMAVESVDGKLSLCRKVIMKQELMRPSLRIPQLVLCEDHRAGMVKHQSCPGCGFFCRAGTFMECQPDGNISHRFHRSCASLIRGQVYCPHCGEEASHAKEVTIPKPDCVASVPTAAVPAHKRDNALMDRVKLDSVMVDGAGDLAKESLESVLNALEDGKYKKFKLPPKQLYFSAKRGELQRILHMLVEGVDPNLRMDSEKRKTPLHCAAEEGHKDICHVLVQAGANLDMCDIEQRTPLMYACNNNHLENVKYLLKAGASSAHKDMRGSTCLHLAARAGHTNVLQYLLSLPSIDVNCKDDGGWAPLTWATENMRLEQVKMLISAGADVQIRDKEENLCLHWAAFSGCDEIAQLLLDHRSDLHAVNVHGDTPLHIAVRQNQLDCVMLFLSRGADVNLKNRDGETPLGCCNINSKMWTILNTNKKLTDARRGRESLRERLLCRDVSRGYEDIPVPCVNGVDHEPCPSNFKYIPENCFTSQVNIDENIKHLQHCSCKDDCASSSCICGQLSMHCWYGKDGRLLKEFCRDDPPFLFECNHACSCWRTCRNRVIQNGLRLRLQVFRTERMGWGVRTLQDIPEGGFVCEFAGEIISDGEANIRENDSYMFNLDNKVGEAYCIDGQFYGNVSRFMNHLCEPNLFPVRVFTKHQDMRFPRIAFFASKHIQAGDELGFDYGDHYWQIKKKYFRCQCGSGKCRYSEVVPDRNHAESSAQTLSNQTVAQVEHIQSSNTAIAITQP

Target ORF information:

RefSeq Version	XM_005171778.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171778.3

ORF Insert Sequence:

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG ACAGGGTTAA ATTGGACTCT 
GTCATGGTTG ATGGAGCAGG AGATCTTGCT AAAGAATCCC TGGAAAGTGT TTTAAATGCA 
TTGGAGGATG GCAAGTATAA GAAATTCAAG TTGCCCCCAA AGCAGCTTTA TTTCTCTGCT 
AAGAGAGGAG AGCTGCAAAG AATCTTGCAT ATGCTGGTGG AAGGTGTGGA CCCAAACTTG 
CGGATGGACA GCGAGAAGAG GAAGACTCCA CTACATTGTG CTGCTGAGGA AGGCCATAAA 
GACATCTGCC ATGTGCTGGT GCAGGCTGGT GCGAACCTAG ACATGTGTGA CATTGAGCAG 
CGAACGCCTT TGATGTACGC CTGTAACAAC AATCATCTAG AAAATGTAAA ATACTTGCTC 
AAAGCTGGTG CTTCTTCTGC ACATAAGGAT ATGAGAGGCT CTACATGTCT TCATTTGGCA 
GCCAGAGCAG GACATACCAA TGTGCTGCAG TATCTGCTCT CCCTGCCATC CATAGATGTC 
AACTGTAAGG ATGATGGCGG ATGGGCTCCT CTCACCTGGG CCACAGAGAA CATGCGTCTG 
GAGCAGGTGA AGATGCTGAT TTCAGCAGGA GCTGATGTCC AAATAAGAGA TAAGGAGGAG 
AATTTGTGCC TCCACTGGGC TGCTTTCTCT GGCTGTGATG AAATCGCTCA GCTGCTGCTG 
GACCACAGAT CTGACCTGCA CGCGGTGAAC GTCCATGGAG ACACTCCTCT TCACATCGCT 
GTCAGACAGA ACCAACTGGA CTGCGTCATG TTGTTTCTGT CTCGAGGAGC GGATGTAAAC 
CTGAAGAACA GAGATGGCGA AACTCCTTTG GGCTGCTGTA ATATAAACTC CAAAATGTGG 
ACAATCCTGA ACACCAATAA GAAGCTGACT GATGCGAGGA GAGGACGAGA AAGCCTGAGA 
GAGAGACTCC TCTGCAGAGA TGTGTCCCGA GGCTATGAGG ACATTCCTGT GCCCTGTGTG 
AATGGGGTGG ACCACGAGCC CTGCCCCAGC AACTTCAAAT ACATTCCGGA GAACTGCTTT 
ACCTCCCAAG TAAATATAGA TGAGAACATA AAGCACTTAC AGCACTGCAG TTGTAAAGAT 
GACTGTGCGT CTAGCAGCTG TATCTGTGGC CAGCTCAGCA TGCACTGCTG GTATGGCAAG 
GATGGCCGAT TGCTAAAAGA ATTCTGCCGG GATGATCCAC CCTTTCTGTT CGAGTGTAAC 
CACGCCTGTT CCTGTTGGAG AACGTGCCGG AATAGAGTCA TACAGAATGG CCTTCGGCTC 
AGGCTGCAGG TGTTTAGGAC TGAGAGGATG GGTTGGGGAG TTCGAACACT GCAGGATATT 
CCTGAGGGAG GTTTTGTATG CGAGTTTGCT GGTGAAATAA TTTCCGATGG AGAAGCTAAC 
ATTCGGGAAA ACGACTCCTA CATGTTCAAC CTAGACAATA AGGTTGGAGA GGCCTACTGC 
ATTGATGGAC AATTTTATGG TAATGTTAGC AGGTTTATGA ATCATCTTTG TGAGCCCAAT 
CTATTCCCAG TACGAGTGTT CACCAAACAT CAAGACATGC GCTTTCCTCG TATTGCATTC 
TTTGCAAGCA AGCATATTCA AGCTGGAGAT GAGCTTGGGT TTGACTATGG GGATCATTAC 
TGGCAAATCA AGAAAAAGTA CTTCCGCTGC CAATGTGGCT CTGGAAAATG TAGGTACTCT 
GAGGTCGTTC CGGACCGGAA CCATGCAGAA AGCTCAGCCC AAACCCTCAG TAATCAGACT 
GTTGCTCAGG TGGAACACAT TCAGTCTTCT AACACTGCTA TAGCAATAAC TCAACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1a ( XM_005171778.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171835.1 Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10406
Clone ID Related Accession (Same CDS sequence)	XM_005171778.3 , XM_005171778.4
Accession Version	XM_005171778.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171778.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG ACAGGGTTAA ATTGGACTCT 
GTCATGGTTG ATGGAGCAGG AGATCTTGCT AAAGAATCCC TGGAAAGTGT TTTAAATGCA 
TTGGAGGATG GCAAGTATAA GAAATTCAAG TTGCCCCCAA AGCAGCTTTA TTTCTCTGCT 
AAGAGAGGAG AGCTGCAAAG AATCTTGCAT ATGCTGGTGG AAGGTGTGGA CCCAAACTTG 
CGGATGGACA GCGAGAAGAG GAAGACTCCA CTACATTGTG CTGCTGAGGA AGGCCATAAA 
GACATCTGCC ATGTGCTGGT GCAGGCTGGT GCGAACCTAG ACATGTGTGA CATTGAGCAG 
CGAACGCCTT TGATGTACGC CTGTAACAAC AATCATCTAG AAAATGTAAA ATACTTGCTC 
AAAGCTGGTG CTTCTTCTGC ACATAAGGAT ATGAGAGGCT CTACATGTCT TCATTTGGCA 
GCCAGAGCAG GACATACCAA TGTGCTGCAG TATCTGCTCT CCCTGCCATC CATAGATGTC 
AACTGTAAGG ATGATGGCGG ATGGGCTCCT CTCACCTGGG CCACAGAGAA CATGCGTCTG 
GAGCAGGTGA AGATGCTGAT TTCAGCAGGA GCTGATGTCC AAATAAGAGA TAAGGAGGAG 
AATTTGTGCC TCCACTGGGC TGCTTTCTCT GGCTGTGATG AAATCGCTCA GCTGCTGCTG 
GACCACAGAT CTGACCTGCA CGCGGTGAAC GTCCATGGAG ACACTCCTCT TCACATCGCT 
GTCAGACAGA ACCAACTGGA CTGCGTCATG TTGTTTCTGT CTCGAGGAGC GGATGTAAAC 
CTGAAGAACA GAGATGGCGA AACTCCTTTG GGCTGCTGTA ATATAAACTC CAAAATGTGG 
ACAATCCTGA ACACCAATAA GAAGCTGACT GATGCGAGGA GAGGACGAGA AAGCCTGAGA 
GAGAGACTCC TCTGCAGAGA TGTGTCCCGA GGCTATGAGG ACATTCCTGT GCCCTGTGTG 
AATGGGGTGG ACCACGAGCC CTGCCCCAGC AACTTCAAAT ACATTCCGGA GAACTGCTTT 
ACCTCCCAAG TAAATATAGA TGAGAACATA AAGCACTTAC AGCACTGCAG TTGTAAAGAT 
GACTGTGCGT CTAGCAGCTG TATCTGTGGC CAGCTCAGCA TGCACTGCTG GTATGGCAAG 
GATGGCCGAT TGCTAAAAGA ATTCTGCCGG GATGATCCAC CCTTTCTGTT CGAGTGTAAC 
CACGCCTGTT CCTGTTGGAG AACGTGCCGG AATAGAGTCA TACAGAATGG CCTTCGGCTC 
AGGCTGCAGG TGTTTAGGAC TGAGAGGATG GGTTGGGGAG TTCGAACACT GCAGGATATT 
CCTGAGGGAG GTTTTGTATG CGAGTTTGCT GGTGAAATAA TTTCCGATGG AGAAGCTAAC 
ATTCGGGAAA ACGACTCCTA CATGTTCAAC CTAGACAATA AGGTTGGAGA GGCCTACTGC 
ATTGATGGAC AATTTTATGG TAATGTTAGC AGGTTTATGA ATCATCTTTG TGAGCCCAAT 
CTATTCCCAG TACGAGTGTT CACCAAACAT CAAGACATGC GCTTTCCTCG TATTGCATTC 
TTTGCAAGCA AGCATATTCA AGCTGGAGAT GAGCTTGGGT TTGACTATGG GGATCATTAC 
TGGCAAATCA AGAAAAAGTA CTTCCGCTGC CAATGTGGCT CTGGAAAATG TAGGTACTCT 
GAGGTCGTTC CGGACCGGAA CCATGCAGAA AGCTCAGCCC AAACCCTCAG TAATCAGACT 
GTTGCTCAGG TGGAACACAT TCAGTCTTCT AACACTGCTA TAGCAATAAC TCAACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171835.1
CDS	173..3352
Translation	MAKTKKGMDSLADPQRKGKRPKSLGASPKASKQLSASGSRKSTGSSFQLLSGQTSLKPPAENIISGNHSSVQQDKPETLEKQSFGSDNADKRIPVVSVPEAAHEEDEQREPEGVKVVKPAPPRLAWKTMVRSAPAPPALKTLNRERSGYERDVESSPTQHLSLPQELKDSTQTAPKKKKRKMGLYNFVPKKKPKGSKKANVSLSSSNLQEALRMTGSKGGQMSIEEAFRNKASSEKPEKQTSTAVIEDAEPHQNKETEDAPEGPAEEYTELPLHALAHDSLFTTIPTGLREDKENMETDDTLEPPLCSCRMETPKNQDVVTLAEGKCMAVESVDGKLSLCRKVIMKQELMRPSLRIPQLVLCEDHRAGMVKHQSCPGCGFFCRAGTFMECQPDGNISHRFHRSCASLIRGQVYCPHCGEEASHAKEVTIPKPDCVASVPTAAVPAHKRDNALMDRVKLDSVMVDGAGDLAKESLESVLNALEDGKYKKFKLPPKQLYFSAKRGELQRILHMLVEGVDPNLRMDSEKRKTPLHCAAEEGHKDICHVLVQAGANLDMCDIEQRTPLMYACNNNHLENVKYLLKAGASSAHKDMRGSTCLHLAARAGHTNVLQYLLSLPSIDVNCKDDGGWAPLTWATENMRLEQVKMLISAGADVQIRDKEENLCLHWAAFSGCDEIAQLLLDHRSDLHAVNVHGDTPLHIAVRQNQLDCVMLFLSRGADVNLKNRDGETPLGCCNINSKMWTILNTNKKLTDARRGRESLRERLLCRDVSRGYEDIPVPCVNGVDHEPCPSNFKYIPENCFTSQVNIDENIKHLQHCSCKDDCASSSCICGQLSMHCWYGKDGRLLKEFCRDDPPFLFECNHACSCWRTCRNRVIQNGLRLRLQVFRTERMGWGVRTLQDIPEGGFVCEFAGEIISDGEANIRENDSYMFNLDNKVGEAYCIDGQFYGNVSRFMNHLCEPNLFPVRVFTKHQDMRFPRIAFFASKHIQAGDELGFDYGDHYWQIKKKYFRCQCGSGKCRYSEVVPDRNHAESSAQTLSNQTVAQVEHIQSSNTAIAITQP

Target ORF information:

RefSeq Version	XM_005171778.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171778.4

ORF Insert Sequence:

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG ACAGGGTTAA ATTGGACTCT 
GTCATGGTTG ATGGAGCAGG AGATCTTGCT AAAGAATCCC TGGAAAGTGT TTTAAATGCA 
TTGGAGGATG GCAAGTATAA GAAATTCAAG TTGCCCCCAA AGCAGCTTTA TTTCTCTGCT 
AAGAGAGGAG AGCTGCAAAG AATCTTGCAT ATGCTGGTGG AAGGTGTGGA CCCAAACTTG 
CGGATGGACA GCGAGAAGAG GAAGACTCCA CTACATTGTG CTGCTGAGGA AGGCCATAAA 
GACATCTGCC ATGTGCTGGT GCAGGCTGGT GCGAACCTAG ACATGTGTGA CATTGAGCAG 
CGAACGCCTT TGATGTACGC CTGTAACAAC AATCATCTAG AAAATGTAAA ATACTTGCTC 
AAAGCTGGTG CTTCTTCTGC ACATAAGGAT ATGAGAGGCT CTACATGTCT TCATTTGGCA 
GCCAGAGCAG GACATACCAA TGTGCTGCAG TATCTGCTCT CCCTGCCATC CATAGATGTC 
AACTGTAAGG ATGATGGCGG ATGGGCTCCT CTCACCTGGG CCACAGAGAA CATGCGTCTG 
GAGCAGGTGA AGATGCTGAT TTCAGCAGGA GCTGATGTCC AAATAAGAGA TAAGGAGGAG 
AATTTGTGCC TCCACTGGGC TGCTTTCTCT GGCTGTGATG AAATCGCTCA GCTGCTGCTG 
GACCACAGAT CTGACCTGCA CGCGGTGAAC GTCCATGGAG ACACTCCTCT TCACATCGCT 
GTCAGACAGA ACCAACTGGA CTGCGTCATG TTGTTTCTGT CTCGAGGAGC GGATGTAAAC 
CTGAAGAACA GAGATGGCGA AACTCCTTTG GGCTGCTGTA ATATAAACTC CAAAATGTGG 
ACAATCCTGA ACACCAATAA GAAGCTGACT GATGCGAGGA GAGGACGAGA AAGCCTGAGA 
GAGAGACTCC TCTGCAGAGA TGTGTCCCGA GGCTATGAGG ACATTCCTGT GCCCTGTGTG 
AATGGGGTGG ACCACGAGCC CTGCCCCAGC AACTTCAAAT ACATTCCGGA GAACTGCTTT 
ACCTCCCAAG TAAATATAGA TGAGAACATA AAGCACTTAC AGCACTGCAG TTGTAAAGAT 
GACTGTGCGT CTAGCAGCTG TATCTGTGGC CAGCTCAGCA TGCACTGCTG GTATGGCAAG 
GATGGCCGAT TGCTAAAAGA ATTCTGCCGG GATGATCCAC CCTTTCTGTT CGAGTGTAAC 
CACGCCTGTT CCTGTTGGAG AACGTGCCGG AATAGAGTCA TACAGAATGG CCTTCGGCTC 
AGGCTGCAGG TGTTTAGGAC TGAGAGGATG GGTTGGGGAG TTCGAACACT GCAGGATATT 
CCTGAGGGAG GTTTTGTATG CGAGTTTGCT GGTGAAATAA TTTCCGATGG AGAAGCTAAC 
ATTCGGGAAA ACGACTCCTA CATGTTCAAC CTAGACAATA AGGTTGGAGA GGCCTACTGC 
ATTGATGGAC AATTTTATGG TAATGTTAGC AGGTTTATGA ATCATCTTTG TGAGCCCAAT 
CTATTCCCAG TACGAGTGTT CACCAAACAT CAAGACATGC GCTTTCCTCG TATTGCATTC 
TTTGCAAGCA AGCATATTCA AGCTGGAGAT GAGCTTGGGT TTGACTATGG GGATCATTAC 
TGGCAAATCA AGAAAAAGTA CTTCCGCTGC CAATGTGGCT CTGGAAAATG TAGGTACTCT 
GAGGTCGTTC CGGACCGGAA CCATGCAGAA AGCTCAGCCC AAACCCTCAG TAATCAGACT 
GTTGCTCAGG TGGAACACAT TCAGTCTTCT AACACTGCTA TAGCAATAAC TCAACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1a ( XM_005171779.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171836.1 Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10407
Clone ID Related Accession (Same CDS sequence)	XM_005171779.3 , XM_005171779.4
Accession Version	XM_005171779.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG AGGTTAAATT GGACTCTGTC 
ATGGTTGATG GAGCAGGAGA TCTTGCTAAA GAATCCCTGG AAAGTGTTTT AAATGCATTG 
GAGGATGGCA AGTATAAGAA ATTCAAGTTG CCCCCAAAGC AGCTTTATTT CTCTGCTAAG 
AGAGGAGAGC TGCAAAGAAT CTTGCATATG CTGGTGGAAG GTGTGGACCC AAACTTGCGG 
ATGGACAGCG AGAAGAGGAA GACTCCACTA CATTGTGCTG CTGAGGAAGG CCATAAAGAC 
ATCTGCCATG TGCTGGTGCA GGCTGGTGCG AACCTAGACA TGTGTGACAT TGAGCAGCGA 
ACGCCTTTGA TGTACGCCTG TAACAACAAT CATCTAGAAA ATGTAAAATA CTTGCTCAAA 
GCTGGTGCTT CTTCTGCACA TAAGGATATG AGAGGCTCTA CATGTCTTCA TTTGGCAGCC 
AGAGCAGGAC ATACCAATGT GCTGCAGTAT CTGCTCTCCC TGCCATCCAT AGATGTCAAC 
TGTAAGGATG ATGGCGGATG GGCTCCTCTC ACCTGGGCCA CAGAGAACAT GCGTCTGGAG 
CAGGTGAAGA TGCTGATTTC AGCAGGAGCT GATGTCCAAA TAAGAGATAA GGAGGAGAAT 
TTGTGCCTCC ACTGGGCTGC TTTCTCTGGC TGTGATGAAA TCGCTCAGCT GCTGCTGGAC 
CACAGATCTG ACCTGCACGC GGTGAACGTC CATGGAGACA CTCCTCTTCA CATCGCTGTC 
AGACAGAACC AACTGGACTG CGTCATGTTG TTTCTGTCTC GAGGAGCGGA TGTAAACCTG 
AAGAACAGAG ATGGCGAAAC TCCTTTGGGC TGCTGTAATA TAAACTCCAA AATGTGGACA 
ATCCTGAACA CCAATAAGAA GCTGACTGAT GCGAGGAGAG GACGAGAAAG CCTGAGAGAG 
AGACTCCTCT GCAGAGATGT GTCCCGAGGC TATGAGGACA TTCCTGTGCC CTGTGTGAAT 
GGGGTGGACC ACGAGCCCTG CCCCAGCAAC TTCAAATACA TTCCGGAGAA CTGCTTTACC 
TCCCAAGTAA ATATAGATGA GAACATAAAG CACTTACAGC ACTGCAGTTG TAAAGATGAC 
TGTGCGTCTA GCAGCTGTAT CTGTGGCCAG CTCAGCATGC ACTGCTGGTA TGGCAAGGAT 
GGCCGATTGC TAAAAGAATT CTGCCGGGAT GATCCACCCT TTCTGTTCGA GTGTAACCAC 
GCCTGTTCCT GTTGGAGAAC GTGCCGGAAT AGAGTCATAC AGAATGGCCT TCGGCTCAGG 
CTGCAGGTGT TTAGGACTGA GAGGATGGGT TGGGGAGTTC GAACACTGCA GGATATTCCT 
GAGGGAGGTT TTGTATGCGA GTTTGCTGGT GAAATAATTT CCGATGGAGA AGCTAACATT 
CGGGAAAACG ACTCCTACAT GTTCAACCTA GACAATAAGG TTGGAGAGGC CTACTGCATT 
GATGGACAAT TTTATGGTAA TGTTAGCAGG TTTATGAATC ATCTTTGTGA GCCCAATCTA 
TTCCCAGTAC GAGTGTTCAC CAAACATCAA GACATGCGCT TTCCTCGTAT TGCATTCTTT 
GCAAGCAAGC ATATTCAAGC TGGAGATGAG CTTGGGTTTG ACTATGGGGA TCATTACTGG 
CAAATCAAGA AAAAGTACTT CCGCTGCCAA TGTGGCTCTG GAAAATGTAG GTACTCTGAG 
GTCGTTCCGG ACCGGAACCA TGCAGAAAGC TCAGCCCAAA CCCTCAGTAA TCAGACTGTT 
GCTCAGGTGG AACACATTCA GTCTTCTAAC ACTGCTATAG CAATAACTCA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171836.1
CDS	157..3333
Translation	MAKTKKGMDSLADPQRKGKRPKSLGASPKASKQLSASGSRKSTGSSFQLLSGQTSLKPPAENIISGNHSSVQQDKPETLEKQSFGSDNADKRIPVVSVPEAAHEEDEQREPEGVKVVKPAPPRLAWKTMVRSAPAPPALKTLNRERSGYERDVESSPTQHLSLPQELKDSTQTAPKKKKRKMGLYNFVPKKKPKGSKKANVSLSSSNLQEALRMTGSKGGQMSIEEAFRNKASSEKPEKQTSTAVIEDAEPHQNKETEDAPEGPAEEYTELPLHALAHDSLFTTIPTGLREDKENMETDDTLEPPLCSCRMETPKNQDVVTLAEGKCMAVESVDGKLSLCRKVIMKQELMRPSLRIPQLVLCEDHRAGMVKHQSCPGCGFFCRAGTFMECQPDGNISHRFHRSCASLIRGQVYCPHCGEEASHAKEVTIPKPDCVASVPTAAVPAHKRDNALMEVKLDSVMVDGAGDLAKESLESVLNALEDGKYKKFKLPPKQLYFSAKRGELQRILHMLVEGVDPNLRMDSEKRKTPLHCAAEEGHKDICHVLVQAGANLDMCDIEQRTPLMYACNNNHLENVKYLLKAGASSAHKDMRGSTCLHLAARAGHTNVLQYLLSLPSIDVNCKDDGGWAPLTWATENMRLEQVKMLISAGADVQIRDKEENLCLHWAAFSGCDEIAQLLLDHRSDLHAVNVHGDTPLHIAVRQNQLDCVMLFLSRGADVNLKNRDGETPLGCCNINSKMWTILNTNKKLTDARRGRESLRERLLCRDVSRGYEDIPVPCVNGVDHEPCPSNFKYIPENCFTSQVNIDENIKHLQHCSCKDDCASSSCICGQLSMHCWYGKDGRLLKEFCRDDPPFLFECNHACSCWRTCRNRVIQNGLRLRLQVFRTERMGWGVRTLQDIPEGGFVCEFAGEIISDGEANIRENDSYMFNLDNKVGEAYCIDGQFYGNVSRFMNHLCEPNLFPVRVFTKHQDMRFPRIAFFASKHIQAGDELGFDYGDHYWQIKKKYFRCQCGSGKCRYSEVVPDRNHAESSAQTLSNQTVAQVEHIQSSNTAIAITQP

Target ORF information:

RefSeq Version	XM_005171779.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171779.3

ORF Insert Sequence:

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG AGGTTAAATT GGACTCTGTC 
ATGGTTGATG GAGCAGGAGA TCTTGCTAAA GAATCCCTGG AAAGTGTTTT AAATGCATTG 
GAGGATGGCA AGTATAAGAA ATTCAAGTTG CCCCCAAAGC AGCTTTATTT CTCTGCTAAG 
AGAGGAGAGC TGCAAAGAAT CTTGCATATG CTGGTGGAAG GTGTGGACCC AAACTTGCGG 
ATGGACAGCG AGAAGAGGAA GACTCCACTA CATTGTGCTG CTGAGGAAGG CCATAAAGAC 
ATCTGCCATG TGCTGGTGCA GGCTGGTGCG AACCTAGACA TGTGTGACAT TGAGCAGCGA 
ACGCCTTTGA TGTACGCCTG TAACAACAAT CATCTAGAAA ATGTAAAATA CTTGCTCAAA 
GCTGGTGCTT CTTCTGCACA TAAGGATATG AGAGGCTCTA CATGTCTTCA TTTGGCAGCC 
AGAGCAGGAC ATACCAATGT GCTGCAGTAT CTGCTCTCCC TGCCATCCAT AGATGTCAAC 
TGTAAGGATG ATGGCGGATG GGCTCCTCTC ACCTGGGCCA CAGAGAACAT GCGTCTGGAG 
CAGGTGAAGA TGCTGATTTC AGCAGGAGCT GATGTCCAAA TAAGAGATAA GGAGGAGAAT 
TTGTGCCTCC ACTGGGCTGC TTTCTCTGGC TGTGATGAAA TCGCTCAGCT GCTGCTGGAC 
CACAGATCTG ACCTGCACGC GGTGAACGTC CATGGAGACA CTCCTCTTCA CATCGCTGTC 
AGACAGAACC AACTGGACTG CGTCATGTTG TTTCTGTCTC GAGGAGCGGA TGTAAACCTG 
AAGAACAGAG ATGGCGAAAC TCCTTTGGGC TGCTGTAATA TAAACTCCAA AATGTGGACA 
ATCCTGAACA CCAATAAGAA GCTGACTGAT GCGAGGAGAG GACGAGAAAG CCTGAGAGAG 
AGACTCCTCT GCAGAGATGT GTCCCGAGGC TATGAGGACA TTCCTGTGCC CTGTGTGAAT 
GGGGTGGACC ACGAGCCCTG CCCCAGCAAC TTCAAATACA TTCCGGAGAA CTGCTTTACC 
TCCCAAGTAA ATATAGATGA GAACATAAAG CACTTACAGC ACTGCAGTTG TAAAGATGAC 
TGTGCGTCTA GCAGCTGTAT CTGTGGCCAG CTCAGCATGC ACTGCTGGTA TGGCAAGGAT 
GGCCGATTGC TAAAAGAATT CTGCCGGGAT GATCCACCCT TTCTGTTCGA GTGTAACCAC 
GCCTGTTCCT GTTGGAGAAC GTGCCGGAAT AGAGTCATAC AGAATGGCCT TCGGCTCAGG 
CTGCAGGTGT TTAGGACTGA GAGGATGGGT TGGGGAGTTC GAACACTGCA GGATATTCCT 
GAGGGAGGTT TTGTATGCGA GTTTGCTGGT GAAATAATTT CCGATGGAGA AGCTAACATT 
CGGGAAAACG ACTCCTACAT GTTCAACCTA GACAATAAGG TTGGAGAGGC CTACTGCATT 
GATGGACAAT TTTATGGTAA TGTTAGCAGG TTTATGAATC ATCTTTGTGA GCCCAATCTA 
TTCCCAGTAC GAGTGTTCAC CAAACATCAA GACATGCGCT TTCCTCGTAT TGCATTCTTT 
GCAAGCAAGC ATATTCAAGC TGGAGATGAG CTTGGGTTTG ACTATGGGGA TCATTACTGG 
CAAATCAAGA AAAAGTACTT CCGCTGCCAA TGTGGCTCTG GAAAATGTAG GTACTCTGAG 
GTCGTTCCGG ACCGGAACCA TGCAGAAAGC TCAGCCCAAA CCCTCAGTAA TCAGACTGTT 
GCTCAGGTGG AACACATTCA GTCTTCTAAC ACTGCTATAG CAATAACTCA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ehmt1a ( XM_005171779.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171836.1 Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10407
Clone ID Related Accession (Same CDS sequence)	XM_005171779.3 , XM_005171779.4
Accession Version	XM_005171779.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-9 specific 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171779.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG AGGTTAAATT GGACTCTGTC 
ATGGTTGATG GAGCAGGAGA TCTTGCTAAA GAATCCCTGG AAAGTGTTTT AAATGCATTG 
GAGGATGGCA AGTATAAGAA ATTCAAGTTG CCCCCAAAGC AGCTTTATTT CTCTGCTAAG 
AGAGGAGAGC TGCAAAGAAT CTTGCATATG CTGGTGGAAG GTGTGGACCC AAACTTGCGG 
ATGGACAGCG AGAAGAGGAA GACTCCACTA CATTGTGCTG CTGAGGAAGG CCATAAAGAC 
ATCTGCCATG TGCTGGTGCA GGCTGGTGCG AACCTAGACA TGTGTGACAT TGAGCAGCGA 
ACGCCTTTGA TGTACGCCTG TAACAACAAT CATCTAGAAA ATGTAAAATA CTTGCTCAAA 
GCTGGTGCTT CTTCTGCACA TAAGGATATG AGAGGCTCTA CATGTCTTCA TTTGGCAGCC 
AGAGCAGGAC ATACCAATGT GCTGCAGTAT CTGCTCTCCC TGCCATCCAT AGATGTCAAC 
TGTAAGGATG ATGGCGGATG GGCTCCTCTC ACCTGGGCCA CAGAGAACAT GCGTCTGGAG 
CAGGTGAAGA TGCTGATTTC AGCAGGAGCT GATGTCCAAA TAAGAGATAA GGAGGAGAAT 
TTGTGCCTCC ACTGGGCTGC TTTCTCTGGC TGTGATGAAA TCGCTCAGCT GCTGCTGGAC 
CACAGATCTG ACCTGCACGC GGTGAACGTC CATGGAGACA CTCCTCTTCA CATCGCTGTC 
AGACAGAACC AACTGGACTG CGTCATGTTG TTTCTGTCTC GAGGAGCGGA TGTAAACCTG 
AAGAACAGAG ATGGCGAAAC TCCTTTGGGC TGCTGTAATA TAAACTCCAA AATGTGGACA 
ATCCTGAACA CCAATAAGAA GCTGACTGAT GCGAGGAGAG GACGAGAAAG CCTGAGAGAG 
AGACTCCTCT GCAGAGATGT GTCCCGAGGC TATGAGGACA TTCCTGTGCC CTGTGTGAAT 
GGGGTGGACC ACGAGCCCTG CCCCAGCAAC TTCAAATACA TTCCGGAGAA CTGCTTTACC 
TCCCAAGTAA ATATAGATGA GAACATAAAG CACTTACAGC ACTGCAGTTG TAAAGATGAC 
TGTGCGTCTA GCAGCTGTAT CTGTGGCCAG CTCAGCATGC ACTGCTGGTA TGGCAAGGAT 
GGCCGATTGC TAAAAGAATT CTGCCGGGAT GATCCACCCT TTCTGTTCGA GTGTAACCAC 
GCCTGTTCCT GTTGGAGAAC GTGCCGGAAT AGAGTCATAC AGAATGGCCT TCGGCTCAGG 
CTGCAGGTGT TTAGGACTGA GAGGATGGGT TGGGGAGTTC GAACACTGCA GGATATTCCT 
GAGGGAGGTT TTGTATGCGA GTTTGCTGGT GAAATAATTT CCGATGGAGA AGCTAACATT 
CGGGAAAACG ACTCCTACAT GTTCAACCTA GACAATAAGG TTGGAGAGGC CTACTGCATT 
GATGGACAAT TTTATGGTAA TGTTAGCAGG TTTATGAATC ATCTTTGTGA GCCCAATCTA 
TTCCCAGTAC GAGTGTTCAC CAAACATCAA GACATGCGCT TTCCTCGTAT TGCATTCTTT 
GCAAGCAAGC ATATTCAAGC TGGAGATGAG CTTGGGTTTG ACTATGGGGA TCATTACTGG 
CAAATCAAGA AAAAGTACTT CCGCTGCCAA TGTGGCTCTG GAAAATGTAG GTACTCTGAG 
GTCGTTCCGG ACCGGAACCA TGCAGAAAGC TCAGCCCAAA CCCTCAGTAA TCAGACTGTT 
GCTCAGGTGG AACACATTCA GTCTTCTAAC ACTGCTATAG CAATAACTCA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171836.1
CDS	173..3349
Translation	MAKTKKGMDSLADPQRKGKRPKSLGASPKASKQLSASGSRKSTGSSFQLLSGQTSLKPPAENIISGNHSSVQQDKPETLEKQSFGSDNADKRIPVVSVPEAAHEEDEQREPEGVKVVKPAPPRLAWKTMVRSAPAPPALKTLNRERSGYERDVESSPTQHLSLPQELKDSTQTAPKKKKRKMGLYNFVPKKKPKGSKKANVSLSSSNLQEALRMTGSKGGQMSIEEAFRNKASSEKPEKQTSTAVIEDAEPHQNKETEDAPEGPAEEYTELPLHALAHDSLFTTIPTGLREDKENMETDDTLEPPLCSCRMETPKNQDVVTLAEGKCMAVESVDGKLSLCRKVIMKQELMRPSLRIPQLVLCEDHRAGMVKHQSCPGCGFFCRAGTFMECQPDGNISHRFHRSCASLIRGQVYCPHCGEEASHAKEVTIPKPDCVASVPTAAVPAHKRDNALMEVKLDSVMVDGAGDLAKESLESVLNALEDGKYKKFKLPPKQLYFSAKRGELQRILHMLVEGVDPNLRMDSEKRKTPLHCAAEEGHKDICHVLVQAGANLDMCDIEQRTPLMYACNNNHLENVKYLLKAGASSAHKDMRGSTCLHLAARAGHTNVLQYLLSLPSIDVNCKDDGGWAPLTWATENMRLEQVKMLISAGADVQIRDKEENLCLHWAAFSGCDEIAQLLLDHRSDLHAVNVHGDTPLHIAVRQNQLDCVMLFLSRGADVNLKNRDGETPLGCCNINSKMWTILNTNKKLTDARRGRESLRERLLCRDVSRGYEDIPVPCVNGVDHEPCPSNFKYIPENCFTSQVNIDENIKHLQHCSCKDDCASSSCICGQLSMHCWYGKDGRLLKEFCRDDPPFLFECNHACSCWRTCRNRVIQNGLRLRLQVFRTERMGWGVRTLQDIPEGGFVCEFAGEIISDGEANIRENDSYMFNLDNKVGEAYCIDGQFYGNVSRFMNHLCEPNLFPVRVFTKHQDMRFPRIAFFASKHIQAGDELGFDYGDHYWQIKKKYFRCQCGSGKCRYSEVVPDRNHAESSAQTLSNQTVAQVEHIQSSNTAIAITQP

Target ORF information:

RefSeq Version	XM_005171779.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio euchromatic histone-lysine N-methyltransferase 1a (ehmt1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171779.4

ORF Insert Sequence:

ATGGCCAAGA CCAAGAAAGG TATGGACAGC CTTGCAGATC CTCAGAGAAA AGGCAAAAGG 
CCTAAATCTC TCGGTGCTTC TCCAAAAGCC AGTAAACAAC TGAGTGCTTC AGGCAGCAGG 
AAGAGCACAG GGTCCAGCTT TCAGCTTCTC TCTGGTCAAA CATCCCTCAA ACCACCAGCT 
GAAAACATCA TCTCAGGAAA CCACAGCTCT GTCCAACAAG ATAAACCAGA AACTTTAGAG 
AAACAGAGTT TTGGGTCTGA TAATGCTGAC AAGAGAATCC CTGTTGTGTC CGTACCTGAA 
GCCGCACATG AAGAGGATGA GCAGAGAGAA CCTGAGGGGG TTAAAGTGGT GAAGCCTGCT 
CCACCACGTC TAGCTTGGAA AACTATGGTC AGATCAGCAC CAGCTCCACC TGCTCTGAAG 
ACTTTGAACA GGGAGAGAAG TGGGTATGAG AGAGACGTGG AGTCATCACC CACACAGCAT 
CTCAGTTTAC CTCAGGAACT CAAAGACTCC ACACAAACAG CCCCCAAGAA GAAGAAAAGA 
AAAATGGGTT TATATAATTT TGTTCCCAAA AAGAAACCGA AAGGGTCCAA GAAAGCGAAT 
GTGTCGTTAT CCTCCTCAAA TCTGCAGGAA GCTTTACGTA TGACCGGGTC CAAGGGTGGA 
CAGATGTCTA TTGAGGAGGC ATTCAGAAAT AAAGCTAGTT CTGAGAAACC AGAGAAACAG 
ACTTCAACTG CTGTGATAGA GGATGCAGAA CCGCACCAGA ACAAAGAAAC TGAGGATGCT 
CCAGAAGGCC CTGCTGAGGA ATACACAGAG CTGCCTTTAC ATGCTCTGGC TCATGATAGC 
TTGTTCACAA CAATCCCCAC AGGCTTACGG GAGGATAAAG AGAACATGGA AACTGATGAC 
ACGTTAGAGC CTCCACTGTG TAGCTGCCGC ATGGAGACGC CAAAGAACCA GGATGTAGTC 
ACACTGGCTG AGGGCAAGTG CATGGCTGTG GAGAGTGTTG ATGGGAAGCT GAGTTTATGT 
CGGAAAGTGA TCATGAAGCA GGAATTGATG CGTCCATCTC TGAGGATTCC ACAGCTCGTA 
CTGTGTGAGG ATCATCGTGC AGGAATGGTG AAACACCAGA GCTGTCCGGG CTGTGGCTTC 
TTCTGCAGAG CTGGGACATT TATGGAGTGT CAGCCAGATG GGAACATCTC TCACCGCTTC 
CACCGGAGCT GTGCATCTCT GATCAGAGGC CAGGTCTACT GCCCTCACTG CGGTGAGGAG 
GCTAGTCATG CTAAGGAGGT CACGATCCCC AAACCTGACT GTGTGGCTTC AGTGCCAACA 
GCTGCTGTGC CGGCACACAA GAGAGACAAC GCCTTAATGG AGGTTAAATT GGACTCTGTC 
ATGGTTGATG GAGCAGGAGA TCTTGCTAAA GAATCCCTGG AAAGTGTTTT AAATGCATTG 
GAGGATGGCA AGTATAAGAA ATTCAAGTTG CCCCCAAAGC AGCTTTATTT CTCTGCTAAG 
AGAGGAGAGC TGCAAAGAAT CTTGCATATG CTGGTGGAAG GTGTGGACCC AAACTTGCGG 
ATGGACAGCG AGAAGAGGAA GACTCCACTA CATTGTGCTG CTGAGGAAGG CCATAAAGAC 
ATCTGCCATG TGCTGGTGCA GGCTGGTGCG AACCTAGACA TGTGTGACAT TGAGCAGCGA 
ACGCCTTTGA TGTACGCCTG TAACAACAAT CATCTAGAAA ATGTAAAATA CTTGCTCAAA 
GCTGGTGCTT CTTCTGCACA TAAGGATATG AGAGGCTCTA CATGTCTTCA TTTGGCAGCC 
AGAGCAGGAC ATACCAATGT GCTGCAGTAT CTGCTCTCCC TGCCATCCAT AGATGTCAAC 
TGTAAGGATG ATGGCGGATG GGCTCCTCTC ACCTGGGCCA CAGAGAACAT GCGTCTGGAG 
CAGGTGAAGA TGCTGATTTC AGCAGGAGCT GATGTCCAAA TAAGAGATAA GGAGGAGAAT 
TTGTGCCTCC ACTGGGCTGC TTTCTCTGGC TGTGATGAAA TCGCTCAGCT GCTGCTGGAC 
CACAGATCTG ACCTGCACGC GGTGAACGTC CATGGAGACA CTCCTCTTCA CATCGCTGTC 
AGACAGAACC AACTGGACTG CGTCATGTTG TTTCTGTCTC GAGGAGCGGA TGTAAACCTG 
AAGAACAGAG ATGGCGAAAC TCCTTTGGGC TGCTGTAATA TAAACTCCAA AATGTGGACA 
ATCCTGAACA CCAATAAGAA GCTGACTGAT GCGAGGAGAG GACGAGAAAG CCTGAGAGAG 
AGACTCCTCT GCAGAGATGT GTCCCGAGGC TATGAGGACA TTCCTGTGCC CTGTGTGAAT 
GGGGTGGACC ACGAGCCCTG CCCCAGCAAC TTCAAATACA TTCCGGAGAA CTGCTTTACC 
TCCCAAGTAA ATATAGATGA GAACATAAAG CACTTACAGC ACTGCAGTTG TAAAGATGAC 
TGTGCGTCTA GCAGCTGTAT CTGTGGCCAG CTCAGCATGC ACTGCTGGTA TGGCAAGGAT 
GGCCGATTGC TAAAAGAATT CTGCCGGGAT GATCCACCCT TTCTGTTCGA GTGTAACCAC 
GCCTGTTCCT GTTGGAGAAC GTGCCGGAAT AGAGTCATAC AGAATGGCCT TCGGCTCAGG 
CTGCAGGTGT TTAGGACTGA GAGGATGGGT TGGGGAGTTC GAACACTGCA GGATATTCCT 
GAGGGAGGTT TTGTATGCGA GTTTGCTGGT GAAATAATTT CCGATGGAGA AGCTAACATT 
CGGGAAAACG ACTCCTACAT GTTCAACCTA GACAATAAGG TTGGAGAGGC CTACTGCATT 
GATGGACAAT TTTATGGTAA TGTTAGCAGG TTTATGAATC ATCTTTGTGA GCCCAATCTA 
TTCCCAGTAC GAGTGTTCAC CAAACATCAA GACATGCGCT TTCCTCGTAT TGCATTCTTT 
GCAAGCAAGC ATATTCAAGC TGGAGATGAG CTTGGGTTTG ACTATGGGGA TCATTACTGG 
CAAATCAAGA AAAAGTACTT CCGCTGCCAA TGTGGCTCTG GAAAATGTAG GTACTCTGAG 
GTCGTTCCGG ACCGGAACCA TGCAGAAAGC TCAGCCCAAA CCCTCAGTAA TCAGACTGTT 
GCTCAGGTGG AACACATTCA GTCTTCTAAC ACTGCTATAG CAATAACTCA ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histological and transcriptomic effects of 17?-methyltestosterone on zebrafish gonad development.
BMC genomics18(1)557(2017 Jul)
Lee SLJ,Horsfield JA,Black MA,Rutherford K,Fisher A,Gemmell NJ

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Genome-wide survey and developmental expression mapping of zebrafish SET domain-containing genes.
PloS one3(1)e1499(2008 Jan)
Sun XJ,Xu PF,Zhou T,Hu M,Fu CT,Zhang Y,Jin Y,Chen Y,Chen SJ,Huang QH,Liu TX,Chen Z

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ehmt1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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