COL28A2B cDNA ORF clone, Danio rerio(Zebrafish)

The following COL28A2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL28A2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa11397 XM_021476979.1
Latest version!
Danio rerio collagen, type XXVIII, alpha 2b (col28a2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa11397 XM_017356552.1
Latest version!
Danio rerio collagen, type XXVIII, alpha 2b (col28a2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa11397
    Clone ID Related Accession (Same CDS sequence) XM_021476979.1 , XM_017356552.1
    Accession Version XM_021476979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-1(XXVIII) chain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) and transcript sequence (AM947018.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 60 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATGCTGC GGCTGTGTGT GCGTTGGCTG GTTTTGCTGT CGGCAGCGCA GCTTGTTTTG 
    GCTCAGCTGA AGGATTTTGA TGATGAAGAT GAAGATCCTG AAGATTTTGT AGAAGACAAT
    AGGAATGGAA GACGTAAAAG TAAACCAATC ACCTCAAATG TGATCCCCAA AAACAAAGAC
    GAGAACTGTA ATTTGGAACT GGCGTTTTTG GTGGACAGCT CTGAAAGTGC CAAAGACAAC
    CATGGCCAGG AGAAGAGCTT CGTTACTGAT CTTGTCAACC GTATCCCGAA CATCCGTCTC
    CAAACAGGCC AGGGCTTGAA CTTCAGATCA GCTCTCCTTC AATACAGCAG TCATGTGATC
    ACAGAGCAGT CCTTTAAAGA CTGGAGGGGT GTGCCCAGCT TCCAGAGCCG CGTCGCCTCT
    ATCCCGTTCA TCGGGCATGG AACCTATACC ACCTACGCCA TCACCAACCT CACCCGCATC
    TACCTGGAGG AGTCCGGCCC AGGCACGGTC AAGGTGGCTA TTCTGATGTA CGGCGGCGCC
    TCGCATCCCA AGAATCCTGA TATTTTCTCT GCTCTTGCTG ATGCGAAGAA CCAAGGGATT
    AAATTCTTCA TAGTCGGTTT AACATCCGCA GCCAACATGG AGAAGCTGCA GCTTCTGGCC
    AGTGCTCCAG CCAGCCGATA CGTGCACAAT ATACAGGACA AGGGCGTCGT GGACAAGATC
    ATCAGAGAGA TTACTAAGGT GGTTGACGAA GTGTGTCCAT TTTCCCCAAA ATGTACTTGT
    GAGAAAGGAG ACCGAGGACC CAGTGGACCC GCTGGTAAAA AGGGTCGTCC TGGAGATGAC
    GGAAGTCCAG GCCCTAAAGG TCAAAAGGGA GAGGGCGGTT CGAGCGGTGC TCCTGGACGA
    GATGGGACAG AGGGGAAAAC TGGATACAAA GGAGAAAAGG GTGAGCGAGG CGAGTGTGGA
    ACACCAGGAG TGAAAGGAGA CAGGGGTCCT GAGGGTCCTG TTGGCATCAG AGGAAGTCGA
    GGGCTGCAAG GTTTGCCAGG ACCCAATGGA GATGTTGGAC CTGAAGGCCT ACCAGGAAAA
    AAAGGTGAGA GAGGATTTCC CGGACCCCCA GGGATACAAG GAGAGACTGG CATTGGACTT
    CAAGGACCAA AGGGTGATGT GGGATTTCAG GGAAGAATGG GTCCTCCTGG GCTTCCTGGG
    CTTGGGGAGC CAGGTTTACC GGGGCCACAG GGGCCACAAG GTTCGCCAGG AGAAAAGGGC
    CCTCCAGGAG AGGGTTTTCC AGGACCAAAG GGAGAGAGGG GTCTGGAGGG GCCAAGGGGA
    CCCCGAGGAC CAGCAGGTAC TGCCATTAAA GGAGACAAAG GGGACTTTGG TTCTCCTGGG
    CTTCCAGGAC CAGTTGGGCC AATTGGAATA GGGGTACAAG GAGAGAAAGG TATAGAAGGA
    CCGCGTGGAC CCCCTGGAAG CAGAGGACCA CCAGGCGAGG GCTACCCAGG ACCAAAGGGT
    GATCAAGGTT TACCAGGTGA GAGTGGTGCA CCAGGAGAGA GAGGAGCCGG GGAACCAGGA
    GCTAAAGGGG AACCTGGAGC TCCAGGACTG TCAGGATTAC CAGGTTTACC AGGGGAGGAC
    GGAGCTCCTG GACAAAAGGG AGAACCAGGA GCCACTGGCT TAAGAGGTGC TGAAGGAGCA
    GCAGGAGTGG GCATCCAGGG GGAAAAAGGT GATCAGGGTC AGAGGGGAAT CCGTGGATTA
    GCAGGTCCAC CAGGAATAGC TGGACCCTCG GGGTCAAAGG GAGAACCTGG CACCCCTGGG
    CGACAAGGAT TACCTGGGCC ACCGGGACGT GCAATATCAG GACCAAAAGG TGACGTAGGC
    CCACCAGGTC CATCTGGTCC AGTAGGTGAT CCTGGATATG GACTTACTGG ACCCAAGGGG
    GATCGCGGTG ACCCTGGTCT TCCTGGTCTG ACTGGTCTGA AAGGGGATGG ATATCCTGGT
    CCTACAGGCC TTCCTGGTCC TCCAGGTCTT CCAGGTGAAC CAGGTCAAGG AGGCGTAGGA
    ATACCAGGAC CAAAGGGTGA TGTTGGCTTC AGAGGTTTAC CAGGCCCACC TGGACCTCCA
    GGGGAAGGAC TGCAAGGACC ACCTGGCACT GTGGGAAGAC CAGGACCTTC TGGACCTACT
    GGACAACCAG GACAAGGTAT ACAGGGTTCA AAGGGTGATC AGGGCTCACA GGGAGTGACC
    GGGCCAAGAG GATTTCCTGG AGAAGGAATG CCGGGGTCAA AGGGGGATCG TGGAGCTCAG
    GGTGAGAGAG GGGTGAAAGG GGTTAAAGGT GATATGGGAG ATCCTGGTGT TTCTGGACAA
    GCAGGTAGAC CAGGAATCAA AGGAGAATCT GGGCTGACGA GGGACGAGAT AATCAGAATG
    ATCAGAGAGA TTTGTGGCTG TGGAGTCAAA TGTAAAGAGC GTCCTATGGA GCTTGTGTTT
    GTGGTTGACA GCTCTGAGAG CATCGGGCCT GAAAACTTCG AGATCATCAA GGACTTTGTG
    GCGGCGCTGG TCGACAGACT CACCATAGGG CGTAATGCCA CTCGTGTCGG CCTGGTTTTA
    TACAGCCTGG AAGTCCAGCT TGAATTCAAC CTGGCTCGCT ATACAACCAA ACAAGACATT
    AAAGAAGCCA TCCGCAGAGT TCACTACATC GGCGAGGGCA CCTACACCGG CTCTGCCATC
    CACAATGCCA CTCATGAAGC CTTTTACAGC GCCAGGACAG GGGTGAAGAA GGTGGCCATC
    GTCATCACAG ATGGACAGAC GGATAAACGG GAGCCTGTGA AGCTGGAGAT CGCTGTCAGA
    GAGGCGCACG CTGCCAACAT CGAGATGTAC GCTCTGGGAA TTATGAACAT CAGTAATCCT
    ACACAGAACG AGTTTCTCCA GGAGCTCAGT CTTATTGCGT CGCATCCTGA CAGTGAACAT
    ATGTACTACA TTAAAGACTT CAACACATTG CCAGCTCTGG AGTCCAAACT AGTCAATCAG
    CTCTGCGAAG ATGAAAGTAG AGCGCTTATC TTCAACCGCA TTAATGATTT TGAAAACGGA
    TATGGTCTGA ACGGAAACCG CATCAATGGT AACGGCGGGC ATAATTTCGA CACCAGCACA
    TATAAAGGCA GCAGAGAAAA CACAGGAACA CCGTCTGCCA CCAACACCGT GATCACTGAG
    AGAGGCAGAG GAGGCACGTT TGCTTCAACA GCTGGTCCAG ACATTAAACA GGAGGTTGAG
    CGTGTACCTA AAACACCAGA CATAAGAGGA GCTCCTGCAG TCACTGGGAC ATCCACACCT
    AGCAAACCTT CATCAGGGAC AACAACATCA TCATCATCTG TGAACACATA TTCAACTTCA
    ATGCAGTTTA TAGCAGCACC ACGACCACCT TTACCTAAAG AGGTTGTGCT TTTGGACCCC
    CTTTGTAAGC TGCTCCTGGA TCAGGGTCCT TGTCGAGAAT ATAACATCCG ATGGTACTAT
    GTACCACAAG CCAACGCCTG TGCACAGTTC TGGTACGGTG GCTGTGAAGG AAACCGTAAT
    CGCTTTGACA CAGAAGAAGA GTGCAAGAAG ACGTGTGTGG TCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332654.1
    CDS186..3773
    Translation

    Target ORF information:

    RefSeq Version XM_021476979.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type XXVIII, alpha 2b (col28a2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATGCTGC GGCTGTGTGT GCGTTGGCTG GTTTTGCTGT CGGCAGCGCA GCTTGTTTTG 
    GCTCAGCTGA AGGATTTTGA TGATGAAGAT GAAGATCCTG AAGATTTTGT AGAAGACAAT
    AGGAATGGAA GACGTAAAAG TAAACCAATC ACCTCAAATG TGATCCCCAA AAACAAAGAC
    GAGAACTGTA ATTTGGAACT GGCGTTTTTG GTGGACAGCT CTGAAAGTGC CAAAGACAAC
    CATGGCCAGG AGAAGAGCTT CGTTACTGAT CTTGTCAACC GTATCCCGAA CATCCGTCTC
    CAAACAGGCC AGGGCTTGAA CTTCAGATCA GCTCTCCTTC AATACAGCAG TCATGTGATC
    ACAGAGCAGT CCTTTAAAGA CTGGAGGGGT GTGCCCAGCT TCCAGAGCCG CGTCGCCTCT
    ATCCCGTTCA TCGGGCATGG AACCTATACC ACCTACGCCA TCACCAACCT CACCCGCATC
    TACCTGGAGG AGTCCGGCCC AGGCACGGTC AAGGTGGCTA TTCTGATGTA CGGCGGCGCC
    TCGCATCCCA AGAATCCTGA TATTTTCTCT GCTCTTGCTG ATGCGAAGAA CCAAGGGATT
    AAATTCTTCA TAGTCGGTTT AACATCCGCA GCCAACATGG AGAAGCTGCA GCTTCTGGCC
    AGTGCTCCAG CCAGCCGATA CGTGCACAAT ATACAGGACA AGGGCGTCGT GGACAAGATC
    ATCAGAGAGA TTACTAAGGT GGTTGACGAA GTGTGTCCAT TTTCCCCAAA ATGTACTTGT
    GAGAAAGGAG ACCGAGGACC CAGTGGACCC GCTGGTAAAA AGGGTCGTCC TGGAGATGAC
    GGAAGTCCAG GCCCTAAAGG TCAAAAGGGA GAGGGCGGTT CGAGCGGTGC TCCTGGACGA
    GATGGGACAG AGGGGAAAAC TGGATACAAA GGAGAAAAGG GTGAGCGAGG CGAGTGTGGA
    ACACCAGGAG TGAAAGGAGA CAGGGGTCCT GAGGGTCCTG TTGGCATCAG AGGAAGTCGA
    GGGCTGCAAG GTTTGCCAGG ACCCAATGGA GATGTTGGAC CTGAAGGCCT ACCAGGAAAA
    AAAGGTGAGA GAGGATTTCC CGGACCCCCA GGGATACAAG GAGAGACTGG CATTGGACTT
    CAAGGACCAA AGGGTGATGT GGGATTTCAG GGAAGAATGG GTCCTCCTGG GCTTCCTGGG
    CTTGGGGAGC CAGGTTTACC GGGGCCACAG GGGCCACAAG GTTCGCCAGG AGAAAAGGGC
    CCTCCAGGAG AGGGTTTTCC AGGACCAAAG GGAGAGAGGG GTCTGGAGGG GCCAAGGGGA
    CCCCGAGGAC CAGCAGGTAC TGCCATTAAA GGAGACAAAG GGGACTTTGG TTCTCCTGGG
    CTTCCAGGAC CAGTTGGGCC AATTGGAATA GGGGTACAAG GAGAGAAAGG TATAGAAGGA
    CCGCGTGGAC CCCCTGGAAG CAGAGGACCA CCAGGCGAGG GCTACCCAGG ACCAAAGGGT
    GATCAAGGTT TACCAGGTGA GAGTGGTGCA CCAGGAGAGA GAGGAGCCGG GGAACCAGGA
    GCTAAAGGGG AACCTGGAGC TCCAGGACTG TCAGGATTAC CAGGTTTACC AGGGGAGGAC
    GGAGCTCCTG GACAAAAGGG AGAACCAGGA GCCACTGGCT TAAGAGGTGC TGAAGGAGCA
    GCAGGAGTGG GCATCCAGGG GGAAAAAGGT GATCAGGGTC AGAGGGGAAT CCGTGGATTA
    GCAGGTCCAC CAGGAATAGC TGGACCCTCG GGGTCAAAGG GAGAACCTGG CACCCCTGGG
    CGACAAGGAT TACCTGGGCC ACCGGGACGT GCAATATCAG GACCAAAAGG TGACGTAGGC
    CCACCAGGTC CATCTGGTCC AGTAGGTGAT CCTGGATATG GACTTACTGG ACCCAAGGGG
    GATCGCGGTG ACCCTGGTCT TCCTGGTCTG ACTGGTCTGA AAGGGGATGG ATATCCTGGT
    CCTACAGGCC TTCCTGGTCC TCCAGGTCTT CCAGGTGAAC CAGGTCAAGG AGGCGTAGGA
    ATACCAGGAC CAAAGGGTGA TGTTGGCTTC AGAGGTTTAC CAGGCCCACC TGGACCTCCA
    GGGGAAGGAC TGCAAGGACC ACCTGGCACT GTGGGAAGAC CAGGACCTTC TGGACCTACT
    GGACAACCAG GACAAGGTAT ACAGGGTTCA AAGGGTGATC AGGGCTCACA GGGAGTGACC
    GGGCCAAGAG GATTTCCTGG AGAAGGAATG CCGGGGTCAA AGGGGGATCG TGGAGCTCAG
    GGTGAGAGAG GGGTGAAAGG GGTTAAAGGT GATATGGGAG ATCCTGGTGT TTCTGGACAA
    GCAGGTAGAC CAGGAATCAA AGGAGAATCT GGGCTGACGA GGGACGAGAT AATCAGAATG
    ATCAGAGAGA TTTGTGGCTG TGGAGTCAAA TGTAAAGAGC GTCCTATGGA GCTTGTGTTT
    GTGGTTGACA GCTCTGAGAG CATCGGGCCT GAAAACTTCG AGATCATCAA GGACTTTGTG
    GCGGCGCTGG TCGACAGACT CACCATAGGG CGTAATGCCA CTCGTGTCGG CCTGGTTTTA
    TACAGCCTGG AAGTCCAGCT TGAATTCAAC CTGGCTCGCT ATACAACCAA ACAAGACATT
    AAAGAAGCCA TCCGCAGAGT TCACTACATC GGCGAGGGCA CCTACACCGG CTCTGCCATC
    CACAATGCCA CTCATGAAGC CTTTTACAGC GCCAGGACAG GGGTGAAGAA GGTGGCCATC
    GTCATCACAG ATGGACAGAC GGATAAACGG GAGCCTGTGA AGCTGGAGAT CGCTGTCAGA
    GAGGCGCACG CTGCCAACAT CGAGATGTAC GCTCTGGGAA TTATGAACAT CAGTAATCCT
    ACACAGAACG AGTTTCTCCA GGAGCTCAGT CTTATTGCGT CGCATCCTGA CAGTGAACAT
    ATGTACTACA TTAAAGACTT CAACACATTG CCAGCTCTGG AGTCCAAACT AGTCAATCAG
    CTCTGCGAAG ATGAAAGTAG AGCGCTTATC TTCAACCGCA TTAATGATTT TGAAAACGGA
    TATGGTCTGA ACGGAAACCG CATCAATGGT AACGGCGGGC ATAATTTCGA CACCAGCACA
    TATAAAGGCA GCAGAGAAAA CACAGGAACA CCGTCTGCCA CCAACACCGT GATCACTGAG
    AGAGGCAGAG GAGGCACGTT TGCTTCAACA GCTGGTCCAG ACATTAAACA GGAGGTTGAG
    CGTGTACCTA AAACACCAGA CATAAGAGGA GCTCCTGCAG TCACTGGGAC ATCCACACCT
    AGCAAACCTT CATCAGGGAC AACAACATCA TCATCATCTG TGAACACATA TTCAACTTCA
    ATGCAGTTTA TAGCAGCACC ACGACCACCT TTACCTAAAG AGGTTGTGCT TTTGGACCCC
    CTTTGTAAGC TGCTCCTGGA TCAGGGTCCT TGTCGAGAAT ATAACATCCG ATGGTACTAT
    GTACCACAAG CCAACGCCTG TGCACAGTTC TGGTACGGTG GCTGTGAAGG AAACCGTAAT
    CGCTTTGACA CAGAAGAAGA GTGCAAGAAG ACGTGTGTGG TCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11397
    Clone ID Related Accession (Same CDS sequence) XM_021476979.1 , XM_017356552.1
    Accession Version XM_017356552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-1(XXVIII) chain
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) and transcript sequences (AM947017.1 and AM947018.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 129 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATGCTGC GGCTGTGTGT GCGTTGGCTG GTTTTGCTGT CGGCAGCGCA GCTTGTTTTG 
    GCTCAGCTGA AGGATTTTGA TGATGAAGAT GAAGATCCTG AAGATTTTGT AGAAGACAAT
    AGGAATGGAA GACGTAAAAG TAAACCAATC ACCTCAAATG TGATCCCCAA AAACAAAGAC
    GAGAACTGTA ATTTGGAACT GGCGTTTTTG GTGGACAGCT CTGAAAGTGC CAAAGACAAC
    CATGGCCAGG AGAAGAGCTT CGTTACTGAT CTTGTCAACC GTATCCCGAA CATCCGTCTC
    CAAACAGGCC AGGGCTTGAA CTTCAGATCA GCTCTCCTTC AATACAGCAG TCATGTGATC
    ACAGAGCAGT CCTTTAAAGA CTGGAGGGGT GTGCCCAGCT TCCAGAGCCG CGTCGCCTCT
    ATCCCGTTCA TCGGGCATGG AACCTATACC ACCTACGCCA TCACCAACCT CACCCGCATC
    TACCTGGAGG AGTCCGGCCC AGGCACGGTC AAGGTGGCTA TTCTGATGTA CGGCGGCGCC
    TCGCATCCCA AGAATCCTGA TATTTTCTCT GCTCTTGCTG ATGCGAAGAA CCAAGGGATT
    AAATTCTTCA TAGTCGGTTT AACATCCGCA GCCAACATGG AGAAGCTGCA GCTTCTGGCC
    AGTGCTCCAG CCAGCCGATA CGTGCACAAT ATACAGGACA AGGGCGTCGT GGACAAGATC
    ATCAGAGAGA TTACTAAGGT GGTTGACGAA GTGTGTCCAT TTTCCCCAAA ATGTACTTGT
    GAGAAAGGAG ACCGAGGACC CAGTGGACCC GCTGGTAAAA AGGGTCGTCC TGGAGATGAC
    GGAAGTCCAG GCCCTAAAGG TCAAAAGGGA GAGGGCGGTT CGAGCGGTGC TCCTGGACGA
    GATGGGACAG AGGGGAAAAC TGGATACAAA GGAGAAAAGG GTGAGCGAGG CGAGTGTGGA
    ACACCAGGAG TGAAAGGAGA CAGGGGTCCT GAGGGTCCTG TTGGCATCAG AGGAAGTCGA
    GGGCTGCAAG GTTTGCCAGG ACCCAATGGA GATGTTGGAC CTGAAGGCCT ACCAGGAAAA
    AAAGGTGAGA GAGGATTTCC CGGACCCCCA GGGATACAAG GAGAGACTGG CATTGGACTT
    CAAGGACCAA AGGGTGATGT GGGATTTCAG GGAAGAATGG GTCCTCCTGG GCTTCCTGGG
    CTTGGGGAGC CAGGTTTACC GGGGCCACAG GGGCCACAAG GTTCGCCAGG AGAAAAGGGC
    CCTCCAGGAG AGGGTTTTCC AGGACCAAAG GGAGAGAGGG GTCTGGAGGG GCCAAGGGGA
    CCCCGAGGAC CAGCAGGTAC TGCCATTAAA GGAGACAAAG GGGACTTTGG TTCTCCTGGG
    CTTCCAGGAC CAGTTGGGCC AATTGGAATA GGGGTACAAG GAGAGAAAGG TATAGAAGGA
    CCGCGTGGAC CCCCTGGAAG CAGAGGACCA CCAGGCGAGG GCTACCCAGG ACCAAAGGGT
    GATCAAGGTT TACCAGGTGA GAGTGGTGCA CCAGGAGAGA GAGGAGCCGG GGAACCAGGA
    GCTAAAGGGG AACCTGGAGC TCCAGGACTG TCAGGATTAC CAGGTTTACC AGGGGAGGAC
    GGAGCTCCTG GACAAAAGGG AGAACCAGGA GCCACTGGCT TAAGAGGTGC TGAAGGAGCA
    GCAGGAGTGG GCATCCAGGG GGAAAAAGGT GATCAGGGTC AGAGGGGAAT CCGTGGATTA
    GCAGGTCCAC CAGGAATAGC TGGACCCTCG GGGTCAAAGG GAGAACCTGG CACCCCTGGG
    CGACAAGGAT TACCTGGGCC ACCGGGACGT GCAATATCAG GACCAAAAGG TGACGTAGGC
    CCACCAGGTC CATCTGGTCC AGTAGGTGAT CCTGGATATG GACTTACTGG ACCCAAGGGG
    GATCGCGGTG ACCCTGGTCT TCCTGGTCTG ACTGGTCTGA AAGGGGATGG ATATCCTGGT
    CCTACAGGCC TTCCTGGTCC TCCAGGTCTT CCAGGTGAAC CAGGTCAAGG AGGCGTAGGA
    ATACCAGGAC CAAAGGGTGA TGTTGGCTTC AGAGGTTTAC CAGGCCCACC TGGACCTCCA
    GGGGAAGGAC TGCAAGGACC ACCTGGCACT GTGGGAAGAC CAGGACCTTC TGGACCTACT
    GGACAACCAG GACAAGGTAT ACAGGGTTCA AAGGGTGATC AGGGCTCACA GGGAGTGACC
    GGGCCAAGAG GATTTCCTGG AGAAGGAATG CCGGGGTCAA AGGGGGATCG TGGAGCTCAG
    GGTGAGAGAG GGGTGAAAGG GGTTAAAGGT GATATGGGAG ATCCTGGTGT TTCTGGACAA
    GCAGGTAGAC CAGGAATCAA AGGAGAATCT GGGCTGACGA GGGACGAGAT AATCAGAATG
    ATCAGAGAGA TTTGTGGCTG TGGAGTCAAA TGTAAAGAGC GTCCTATGGA GCTTGTGTTT
    GTGGTTGACA GCTCTGAGAG CATCGGGCCT GAAAACTTCG AGATCATCAA GGACTTTGTG
    GCGGCGCTGG TCGACAGACT CACCATAGGG CGTAATGCCA CTCGTGTCGG CCTGGTTTTA
    TACAGCCTGG AAGTCCAGCT TGAATTCAAC CTGGCTCGCT ATACAACCAA ACAAGACATT
    AAAGAAGCCA TCCGCAGAGT TCACTACATC GGCGAGGGCA CCTACACCGG CTCTGCCATC
    CACAATGCCA CTCATGAAGC CTTTTACAGC GCCAGGACAG GGGTGAAGAA GGTGGCCATC
    GTCATCACAG ATGGACAGAC GGATAAACGG GAGCCTGTGA AGCTGGAGAT CGCTGTCAGA
    GAGGCGCACG CTGCCAACAT CGAGATGTAC GCTCTGGGAA TTATGAACAT CAGTAATCCT
    ACACAGAACG AGTTTCTCCA GGAGCTCAGT CTTATTGCGT CGCATCCTGA CAGTGAACAT
    ATGTACTACA TTAAAGACTT CAACACATTG CCAGCTCTGG AGTCCAAACT AGTCAATCAG
    CTCTGCGAAG ATGAAAGTAG AGCGCTTATC TTCAACCGCA TTAATGATTT TGAAAACGGA
    TATGGTCTGA ACGGAAACCG CATCAATGGT AACGGCGGGC ATAATTTCGA CACCAGCACA
    TATAAAGGCA GCAGAGAAAA CACAGGAACA CCGTCTGCCA CCAACACCGT GATCACTGAG
    AGAGGCAGAG GAGGCACGTT TGCTTCAACA GCTGGTCCAG ACATTAAACA GGAGGTTGAG
    CGTGTACCTA AAACACCAGA CATAAGAGGA GCTCCTGCAG TCACTGGGAC ATCCACACCT
    AGCAAACCTT CATCAGGGAC AACAACATCA TCATCATCTG TGAACACATA TTCAACTTCA
    ATGCAGTTTA TAGCAGCACC ACGACCACCT TTACCTAAAG AGGTTGTGCT TTTGGACCCC
    CTTTGTAAGC TGCTCCTGGA TCAGGGTCCT TGTCGAGAAT ATAACATCCG ATGGTACTAT
    GTACCACAAG CCAACGCCTG TGCACAGTTC TGGTACGGTG GCTGTGAAGG AAACCGTAAT
    CGCTTTGACA CAGAAGAAGA GTGCAAGAAG ACGTGTGTGG TCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212041.1
    CDS260..3847
    Translation

    Target ORF information:

    RefSeq Version XM_017356552.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type XXVIII, alpha 2b (col28a2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGATGCTGC GGCTGTGTGT GCGTTGGCTG GTTTTGCTGT CGGCAGCGCA GCTTGTTTTG 
    GCTCAGCTGA AGGATTTTGA TGATGAAGAT GAAGATCCTG AAGATTTTGT AGAAGACAAT
    AGGAATGGAA GACGTAAAAG TAAACCAATC ACCTCAAATG TGATCCCCAA AAACAAAGAC
    GAGAACTGTA ATTTGGAACT GGCGTTTTTG GTGGACAGCT CTGAAAGTGC CAAAGACAAC
    CATGGCCAGG AGAAGAGCTT CGTTACTGAT CTTGTCAACC GTATCCCGAA CATCCGTCTC
    CAAACAGGCC AGGGCTTGAA CTTCAGATCA GCTCTCCTTC AATACAGCAG TCATGTGATC
    ACAGAGCAGT CCTTTAAAGA CTGGAGGGGT GTGCCCAGCT TCCAGAGCCG CGTCGCCTCT
    ATCCCGTTCA TCGGGCATGG AACCTATACC ACCTACGCCA TCACCAACCT CACCCGCATC
    TACCTGGAGG AGTCCGGCCC AGGCACGGTC AAGGTGGCTA TTCTGATGTA CGGCGGCGCC
    TCGCATCCCA AGAATCCTGA TATTTTCTCT GCTCTTGCTG ATGCGAAGAA CCAAGGGATT
    AAATTCTTCA TAGTCGGTTT AACATCCGCA GCCAACATGG AGAAGCTGCA GCTTCTGGCC
    AGTGCTCCAG CCAGCCGATA CGTGCACAAT ATACAGGACA AGGGCGTCGT GGACAAGATC
    ATCAGAGAGA TTACTAAGGT GGTTGACGAA GTGTGTCCAT TTTCCCCAAA ATGTACTTGT
    GAGAAAGGAG ACCGAGGACC CAGTGGACCC GCTGGTAAAA AGGGTCGTCC TGGAGATGAC
    GGAAGTCCAG GCCCTAAAGG TCAAAAGGGA GAGGGCGGTT CGAGCGGTGC TCCTGGACGA
    GATGGGACAG AGGGGAAAAC TGGATACAAA GGAGAAAAGG GTGAGCGAGG CGAGTGTGGA
    ACACCAGGAG TGAAAGGAGA CAGGGGTCCT GAGGGTCCTG TTGGCATCAG AGGAAGTCGA
    GGGCTGCAAG GTTTGCCAGG ACCCAATGGA GATGTTGGAC CTGAAGGCCT ACCAGGAAAA
    AAAGGTGAGA GAGGATTTCC CGGACCCCCA GGGATACAAG GAGAGACTGG CATTGGACTT
    CAAGGACCAA AGGGTGATGT GGGATTTCAG GGAAGAATGG GTCCTCCTGG GCTTCCTGGG
    CTTGGGGAGC CAGGTTTACC GGGGCCACAG GGGCCACAAG GTTCGCCAGG AGAAAAGGGC
    CCTCCAGGAG AGGGTTTTCC AGGACCAAAG GGAGAGAGGG GTCTGGAGGG GCCAAGGGGA
    CCCCGAGGAC CAGCAGGTAC TGCCATTAAA GGAGACAAAG GGGACTTTGG TTCTCCTGGG
    CTTCCAGGAC CAGTTGGGCC AATTGGAATA GGGGTACAAG GAGAGAAAGG TATAGAAGGA
    CCGCGTGGAC CCCCTGGAAG CAGAGGACCA CCAGGCGAGG GCTACCCAGG ACCAAAGGGT
    GATCAAGGTT TACCAGGTGA GAGTGGTGCA CCAGGAGAGA GAGGAGCCGG GGAACCAGGA
    GCTAAAGGGG AACCTGGAGC TCCAGGACTG TCAGGATTAC CAGGTTTACC AGGGGAGGAC
    GGAGCTCCTG GACAAAAGGG AGAACCAGGA GCCACTGGCT TAAGAGGTGC TGAAGGAGCA
    GCAGGAGTGG GCATCCAGGG GGAAAAAGGT GATCAGGGTC AGAGGGGAAT CCGTGGATTA
    GCAGGTCCAC CAGGAATAGC TGGACCCTCG GGGTCAAAGG GAGAACCTGG CACCCCTGGG
    CGACAAGGAT TACCTGGGCC ACCGGGACGT GCAATATCAG GACCAAAAGG TGACGTAGGC
    CCACCAGGTC CATCTGGTCC AGTAGGTGAT CCTGGATATG GACTTACTGG ACCCAAGGGG
    GATCGCGGTG ACCCTGGTCT TCCTGGTCTG ACTGGTCTGA AAGGGGATGG ATATCCTGGT
    CCTACAGGCC TTCCTGGTCC TCCAGGTCTT CCAGGTGAAC CAGGTCAAGG AGGCGTAGGA
    ATACCAGGAC CAAAGGGTGA TGTTGGCTTC AGAGGTTTAC CAGGCCCACC TGGACCTCCA
    GGGGAAGGAC TGCAAGGACC ACCTGGCACT GTGGGAAGAC CAGGACCTTC TGGACCTACT
    GGACAACCAG GACAAGGTAT ACAGGGTTCA AAGGGTGATC AGGGCTCACA GGGAGTGACC
    GGGCCAAGAG GATTTCCTGG AGAAGGAATG CCGGGGTCAA AGGGGGATCG TGGAGCTCAG
    GGTGAGAGAG GGGTGAAAGG GGTTAAAGGT GATATGGGAG ATCCTGGTGT TTCTGGACAA
    GCAGGTAGAC CAGGAATCAA AGGAGAATCT GGGCTGACGA GGGACGAGAT AATCAGAATG
    ATCAGAGAGA TTTGTGGCTG TGGAGTCAAA TGTAAAGAGC GTCCTATGGA GCTTGTGTTT
    GTGGTTGACA GCTCTGAGAG CATCGGGCCT GAAAACTTCG AGATCATCAA GGACTTTGTG
    GCGGCGCTGG TCGACAGACT CACCATAGGG CGTAATGCCA CTCGTGTCGG CCTGGTTTTA
    TACAGCCTGG AAGTCCAGCT TGAATTCAAC CTGGCTCGCT ATACAACCAA ACAAGACATT
    AAAGAAGCCA TCCGCAGAGT TCACTACATC GGCGAGGGCA CCTACACCGG CTCTGCCATC
    CACAATGCCA CTCATGAAGC CTTTTACAGC GCCAGGACAG GGGTGAAGAA GGTGGCCATC
    GTCATCACAG ATGGACAGAC GGATAAACGG GAGCCTGTGA AGCTGGAGAT CGCTGTCAGA
    GAGGCGCACG CTGCCAACAT CGAGATGTAC GCTCTGGGAA TTATGAACAT CAGTAATCCT
    ACACAGAACG AGTTTCTCCA GGAGCTCAGT CTTATTGCGT CGCATCCTGA CAGTGAACAT
    ATGTACTACA TTAAAGACTT CAACACATTG CCAGCTCTGG AGTCCAAACT AGTCAATCAG
    CTCTGCGAAG ATGAAAGTAG AGCGCTTATC TTCAACCGCA TTAATGATTT TGAAAACGGA
    TATGGTCTGA ACGGAAACCG CATCAATGGT AACGGCGGGC ATAATTTCGA CACCAGCACA
    TATAAAGGCA GCAGAGAAAA CACAGGAACA CCGTCTGCCA CCAACACCGT GATCACTGAG
    AGAGGCAGAG GAGGCACGTT TGCTTCAACA GCTGGTCCAG ACATTAAACA GGAGGTTGAG
    CGTGTACCTA AAACACCAGA CATAAGAGGA GCTCCTGCAG TCACTGGGAC ATCCACACCT
    AGCAAACCTT CATCAGGGAC AACAACATCA TCATCATCTG TGAACACATA TTCAACTTCA
    ATGCAGTTTA TAGCAGCACC ACGACCACCT TTACCTAAAG AGGTTGTGCT TTTGGACCCC
    CTTTGTAAGC TGCTCCTGGA TCAGGGTCCT TGTCGAGAAT ATAACATCCG ATGGTACTAT
    GTACCACAAG CCAACGCCTG TGCACAGTTC TGGTACGGTG GCTGTGAAGG AAACCGTAAT
    CGCTTTGACA CAGAAGAAGA GTGCAAGAAG ACGTGTGTGG TCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.