efnb2 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	efnb2
Entrez Gene ID	107082588
Full Name	ephrin B2
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015371358.1	XP_015226844.1	ephrin-B2

Pathways from BioSystems
Gene Ontology

The following efnb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efnb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy00495	XM_015371358.1 Latest version!	Cyprinodon variegatus ephrin-B2 (efnb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

efnb2 ( XM_015371358.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015226844.1 Cyprinodon variegatus ephrin-B2 (efnb2), mRNA.

Price & Availability↑

CloneID	OCy00495
Clone ID Related Accession (Same CDS sequence)	XM_015371358.1
Accession Version	XM_015371358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	ephrin-B2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150456.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACT CGATGTGGAG ATATTATTTA GGAGTTTTCT TCCTCGCCTT GGAGCTGGAC 
TTGTGTCGGC CGCTCATCCT GGAGTCCATC TACTGGAACA CCACCAACAC CAAATTTGTT 
CCAGGCCAAG GCGTGGTTTT ATACCCGCAA ATCGGGGACA AGCTGGACAT TGTGTGCCCC 
CGGGTGGACG GCGGGGTGCC CGACGGCGTG GAGTATTACA AGGTTTACAT GGTGCCCAGG 
GAGCAGCTGG AGAGCTGCAC CATCACCAAG GCCGACACGC CGCTGCTGAA CTGCGTGAAG 
CCCGACCAGG ATGTGAAATT CACCCTGAAG TTTCAGGAGT TCAGCCCCAA CCTGTGGGGG 
CTGGAGTTCT TCAAGGGGAA GGACTACTAC ATCATCTCTA CGTCCAACGG CACCATGGAG 
GGCATCGACA ACCAGGAGGG AGGGGTGTGC AAGACCAAGT CCATGAAGAT AGTCATGAAA 
GTGGGACAGA ATCCCTCTGA CCCTGTTTCA CCCAAAGACA GCCCTACGAG AACACCTTAC 
AAACCTCTCG ATGACAACAG GGAGTCGAAC GAAGTTCCCG ACCAACCTGG GAAATCCCAC 
ACAGACGTCG GCGGAGGAGG CGGCAGCAGC GGCGGCGGGA AGTCGTCCAG CGCCATTGGC 
TCCGAGGTGG CCATCTTCGT GGGCATCGCC TCGGGCAGCG TCATCTTCAT CGTCATCATC 
GTCATGCTGG TCCTGCTGCT GCTGAAGTAC CGACGGCGGC ACCGCAAGCA CTCGCCGCAG 
CACACGGCCA CGCTGTCGCT GAGCACGCTG GCCACGCCCA AACGGAGCGG AGGCGGCGGC 
AACAACAACG GCTCGGAGCC CAGCGACATC ATCATCCCGC TCAGGACTGC TGACAGCGTC 
TTCTGCCCGC ACTACGAGAA GGTGAGCGGG GACTACGGAC ACCCCGTCTA CATCGTTCAG 
GAGATGCCTC CTCAGAGCCC CGCCAACATC TACTACAAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015226844.1
CDS	655..1659
Translation	MGDSMWRYYLGVFFLALELDLCRPLILESIYWNTTNTKFVPGQGVVLYPQIGDKLDIVCPRVDGGVPDGVEYYKVYMVPREQLESCTITKADTPLLNCVKPDQDVKFTLKFQEFSPNLWGLEFFKGKDYYIISTSNGTMEGIDNQEGGVCKTKSMKIVMKVGQNPSDPVSPKDSPTRTPYKPLDDNRESNEVPDQPGKSHTDVGGGGGSSGGGKSSSAIGSEVAIFVGIASGSVIFIVIIVMLVLLLLKYRRRHRKHSPQHTATLSLSTLATPKRSGGGGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

Target ORF information:

RefSeq Version	XM_015371358.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus ephrin-B2 (efnb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015371358.1

ORF Insert Sequence:

ATGGGGGACT CGATGTGGAG ATATTATTTA GGAGTTTTCT TCCTCGCCTT GGAGCTGGAC 
TTGTGTCGGC CGCTCATCCT GGAGTCCATC TACTGGAACA CCACCAACAC CAAATTTGTT 
CCAGGCCAAG GCGTGGTTTT ATACCCGCAA ATCGGGGACA AGCTGGACAT TGTGTGCCCC 
CGGGTGGACG GCGGGGTGCC CGACGGCGTG GAGTATTACA AGGTTTACAT GGTGCCCAGG 
GAGCAGCTGG AGAGCTGCAC CATCACCAAG GCCGACACGC CGCTGCTGAA CTGCGTGAAG 
CCCGACCAGG ATGTGAAATT CACCCTGAAG TTTCAGGAGT TCAGCCCCAA CCTGTGGGGG 
CTGGAGTTCT TCAAGGGGAA GGACTACTAC ATCATCTCTA CGTCCAACGG CACCATGGAG 
GGCATCGACA ACCAGGAGGG AGGGGTGTGC AAGACCAAGT CCATGAAGAT AGTCATGAAA 
GTGGGACAGA ATCCCTCTGA CCCTGTTTCA CCCAAAGACA GCCCTACGAG AACACCTTAC 
AAACCTCTCG ATGACAACAG GGAGTCGAAC GAAGTTCCCG ACCAACCTGG GAAATCCCAC 
ACAGACGTCG GCGGAGGAGG CGGCAGCAGC GGCGGCGGGA AGTCGTCCAG CGCCATTGGC 
TCCGAGGTGG CCATCTTCGT GGGCATCGCC TCGGGCAGCG TCATCTTCAT CGTCATCATC 
GTCATGCTGG TCCTGCTGCT GCTGAAGTAC CGACGGCGGC ACCGCAAGCA CTCGCCGCAG 
CACACGGCCA CGCTGTCGCT GAGCACGCTG GCCACGCCCA AACGGAGCGG AGGCGGCGGC 
AACAACAACG GCTCGGAGCC CAGCGACATC ATCATCCCGC TCAGGACTGC TGACAGCGTC 
TTCTGCCCGC ACTACGAGAA GGTGAGCGGG GACTACGGAC ACCCCGTCTA CATCGTTCAG 
GAGATGCCTC CTCAGAGCCC CGCCAACATC TACTACAAGG TCTGA