HMA4 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMA4
Entrez Gene ID	101211188
Full Name	cadmium/zinc-transporting ATPase HMA2-like
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301786.1	NP_001288715.1	cadmium/zinc-transporting ATPase HMA2-like
XM_031888994.1	XP_031744854.1	cadmium/zinc-transporting ATPase HMA2-like isoform X1

Pathways from BioSystems
Gene Ontology

The following HMA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu144845	XM_031888994.1 Latest version!	Cucumis sativus cadmium/zinc-transporting ATPase HMA2-like (HMA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCu36607	NM_001301786.1 Latest version!	Cucumis sativus cadmium/zinc-transporting ATPase HMA2-like (HMA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMA4 ( XM_031888994.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_031744854.1 Cucumis sativus cadmium/zinc-transporting ATPase HMA2-like (HMA4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu144845
Clone ID Related Accession (Same CDS sequence)	XM_031888994.1
Accession Version	XM_031888994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	cadmium/zinc-transporting ATPase HMA2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026655.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA CGGAGGCAGA GGGAAATAAG GGTGGTGGGT TGAAGAAAAG TTACTTTGAT 
GTCTTGGGGA TTTGTTGTTC TTCAGAAATT CCGGTGATTG AGAATATATT GAAGGATATT 
GAAGGGATTA AAGAGATTAG AGTGATTGTT GCAACAAGAA CTGTCATTGT TCTTCATGAT 
GATCTTCTCG TTTCCCAAGC ACAAATTGTT AAAGCCCTGA ACCAAGCAAG ATTTGAAGCG 
AACGTTAGAG CGTACGGAGA TCAGAAAGAT CACCGGAAAA AATGGCCGAG CCCGTATGCG 
GTGGCCAGTG GGCTGTTGCT GCTCTTGTCG TTACTCAAAT ATGTGAATCC TATTTTTCGA 
TGGGTCGCTT TAGCCGCCGT CGCCGCCGGA ATCTTGCCCA TTGTCCTCAA AAGCTTCGCC 
GCCGTTCGCC ATCTCAGAAT CGACATCAAT ATTCTTGCTT TAATTGCAGT AATCGGAACA 
ATTGTGCTGA AGGATTACTT GGAAGCAGCC ACCATCGTCT TCCTCTTCAC CATCGCCGAA 
TGGCTCGAGT CCAGAGCCGC CCACAAGGCC AACGCCGTCA TGTCATCCCT TCTAAGTATT 
GCTCCTCAGA AAGCGGTTCT AGCCGACACT GGCGAAGTTG TCGGCGCCGA CGAGGTCAAA 
TTAGGGACTC TTCTGGCTGT GAAGGCCGGT GAGGACATAC CCATTGATGG AATCGTTGTG 
GAAGGAAAAT GTGAAGTGGA TGAGAAAACA CTTACAGGCG AATCTTTTCC TGTTCCCAAA 
CAAAAGAATT CCACTGTTTG GGCTGGTACC ATTAATCTAA ATGGCTATGT TACTGTGAAG 
ACTACTGCCC TTGCTGAAGA CTGTGTGGTG GCCAAAATGG CTAAGCTGGT TGAGGAGGCC 
CAGAACAATA AATCTAGAAC TCAAAGGTTC ATTGACAAAT GTGCCAAATT CTATACTCCA 
GCTGTTATAA TCATATCAAC TTGCATAGTA GTGATTCCAC TTGCTTTGAG ACTTCCCAAT 
CGCAGCCATT GGTTTCACTT GGCCTTGGTT GTGTTAGTAA GTGCGTGTCC CTGTGCACTC 
ATCCTTTCCA CTCCTGTTGC GTCTTTCTGT GCACTTACTA AGGCAGCAAC ATCTGGTCTT 
TTAATCAAAG GAGGTGACTA CCTTGAAACA CTTGGCAAAA TTAAGATCAT GGCCTTTGAT 
AAAACTGGGA CAATAACAAG AGGTGAATTT ATGGTGACTG AATTTCAAGT TCTTGATAAG 
GACAATATAA GCTTAGACAC ATTGCTGTAC TGGGTGTCGA GCATCGAGAG CAAGTCCAGT 
CATCCAATGG CAGCTGCACT TGTTGACCAT GGAAGATCAC TATCCATTGA TCCCAAACCT 
GAAAATGTGG ACGACTTTCA AAATTTTCCT GGAGAAGGTG TTCATGGGAG AATTGATGGG 
AAAGACATCT ATATTGGAAA TCGAAAAATT GCTACAAGAG CCAACTGCGC AACAGTCCCA 
GAGATCAAAG ATGAAGCAAA GGATGGGAGG ACTGTTGGAT ACATATTCTG TGGAACTATT 
GCCGCTGGAG TCTTTAGTCT GTCTGATTCG TGTAGAACAG GGGCTAAAGA GGCCATGGAT 
GAGCTTAGAT CTCTCGGTAT AAAAACAGCC ATGCTCACTG GAGACAGTTC TGCAGCAGCC 
TTGCAAGCAC AAAAAGAATT GGGAAAGGCT TTACAAGCAG TTCATGCCGA ACTTCTACCT 
CAAGACAAGA CAAGGCTTAT CAATGACTTC AAAAAGGAGG GGCCAACAGC CATGATTGGA 
GATGGTCTAA ACGATGCCCC TGCCTTGGCC ACAGCTGATA TTGGCATATC AATGGGAATC 
TCAGGATTGC TATACTTGGC CTGGCATTTG GTGGTCATCC ACTTGTTTGG GCAGCAGTTC 
TTGCTGATGT TGGTGCCTGT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031744854.1
CDS	225..2171
Translation	MAATEAEGNKGGGLKKSYFDVLGICCSSEIPVIENILKDIEGIKEIRVIVATRTVIVLHDDLLVSQAQIVKALNQARFEANVRAYGDQKDHRKKWPSPYAVASGLLLLLSLLKYVNPIFRWVALAAVAAGILPIVLKSFAAVRHLRIDINILALIAVIGTIVLKDYLEAATIVFLFTIAEWLESRAAHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDKCAKFYTPAVIIISTCIVVIPLALRLPNRSHWFHLALVVLVSACPCALILSTPVASFCALTKAATSGLLIKGGDYLETLGKIKIMAFDKTGTITRGEFMVTEFQVLDKDNISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSIDPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRKIATRANCATVPEIKDEAKDGRTVGYIFCGTIAAGVFSLSDSCRTGAKEAMDELRSLGIKTAMLTGDSSAAALQAQKELGKALQAVHAELLPQDKTRLINDFKKEGPTAMIGDGLNDAPALATADIGISMGISGLLYLAWHLVVIHLFGQQFLLMLVPVC

Target ORF information:

RefSeq Version	XM_031888994.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus cadmium/zinc-transporting ATPase HMA2-like (HMA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031888994.1

ORF Insert Sequence:

ATGGCGGCGA CGGAGGCAGA GGGAAATAAG GGTGGTGGGT TGAAGAAAAG TTACTTTGAT 
GTCTTGGGGA TTTGTTGTTC TTCAGAAATT CCGGTGATTG AGAATATATT GAAGGATATT 
GAAGGGATTA AAGAGATTAG AGTGATTGTT GCAACAAGAA CTGTCATTGT TCTTCATGAT 
GATCTTCTCG TTTCCCAAGC ACAAATTGTT AAAGCCCTGA ACCAAGCAAG ATTTGAAGCG 
AACGTTAGAG CGTACGGAGA TCAGAAAGAT CACCGGAAAA AATGGCCGAG CCCGTATGCG 
GTGGCCAGTG GGCTGTTGCT GCTCTTGTCG TTACTCAAAT ATGTGAATCC TATTTTTCGA 
TGGGTCGCTT TAGCCGCCGT CGCCGCCGGA ATCTTGCCCA TTGTCCTCAA AAGCTTCGCC 
GCCGTTCGCC ATCTCAGAAT CGACATCAAT ATTCTTGCTT TAATTGCAGT AATCGGAACA 
ATTGTGCTGA AGGATTACTT GGAAGCAGCC ACCATCGTCT TCCTCTTCAC CATCGCCGAA 
TGGCTCGAGT CCAGAGCCGC CCACAAGGCC AACGCCGTCA TGTCATCCCT TCTAAGTATT 
GCTCCTCAGA AAGCGGTTCT AGCCGACACT GGCGAAGTTG TCGGCGCCGA CGAGGTCAAA 
TTAGGGACTC TTCTGGCTGT GAAGGCCGGT GAGGACATAC CCATTGATGG AATCGTTGTG 
GAAGGAAAAT GTGAAGTGGA TGAGAAAACA CTTACAGGCG AATCTTTTCC TGTTCCCAAA 
CAAAAGAATT CCACTGTTTG GGCTGGTACC ATTAATCTAA ATGGCTATGT TACTGTGAAG 
ACTACTGCCC TTGCTGAAGA CTGTGTGGTG GCCAAAATGG CTAAGCTGGT TGAGGAGGCC 
CAGAACAATA AATCTAGAAC TCAAAGGTTC ATTGACAAAT GTGCCAAATT CTATACTCCA 
GCTGTTATAA TCATATCAAC TTGCATAGTA GTGATTCCAC TTGCTTTGAG ACTTCCCAAT 
CGCAGCCATT GGTTTCACTT GGCCTTGGTT GTGTTAGTAA GTGCGTGTCC CTGTGCACTC 
ATCCTTTCCA CTCCTGTTGC GTCTTTCTGT GCACTTACTA AGGCAGCAAC ATCTGGTCTT 
TTAATCAAAG GAGGTGACTA CCTTGAAACA CTTGGCAAAA TTAAGATCAT GGCCTTTGAT 
AAAACTGGGA CAATAACAAG AGGTGAATTT ATGGTGACTG AATTTCAAGT TCTTGATAAG 
GACAATATAA GCTTAGACAC ATTGCTGTAC TGGGTGTCGA GCATCGAGAG CAAGTCCAGT 
CATCCAATGG CAGCTGCACT TGTTGACCAT GGAAGATCAC TATCCATTGA TCCCAAACCT 
GAAAATGTGG ACGACTTTCA AAATTTTCCT GGAGAAGGTG TTCATGGGAG AATTGATGGG 
AAAGACATCT ATATTGGAAA TCGAAAAATT GCTACAAGAG CCAACTGCGC AACAGTCCCA 
GAGATCAAAG ATGAAGCAAA GGATGGGAGG ACTGTTGGAT ACATATTCTG TGGAACTATT 
GCCGCTGGAG TCTTTAGTCT GTCTGATTCG TGTAGAACAG GGGCTAAAGA GGCCATGGAT 
GAGCTTAGAT CTCTCGGTAT AAAAACAGCC ATGCTCACTG GAGACAGTTC TGCAGCAGCC 
TTGCAAGCAC AAAAAGAATT GGGAAAGGCT TTACAAGCAG TTCATGCCGA ACTTCTACCT 
CAAGACAAGA CAAGGCTTAT CAATGACTTC AAAAAGGAGG GGCCAACAGC CATGATTGGA 
GATGGTCTAA ACGATGCCCC TGCCTTGGCC ACAGCTGATA TTGGCATATC AATGGGAATC 
TCAGGATTGC TATACTTGGC CTGGCATTTG GTGGTCATCC ACTTGTTTGG GCAGCAGTTC 
TTGCTGATGT TGGTGCCTGT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMA4 ( NM_001301786.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001288715.1 Cucumis sativus cadmium/zinc-transporting ATPase HMA2-like (HMA4), mRNA.

Price & Availability↑

CloneID	OCu36607
Clone ID Related Accession (Same CDS sequence)	NM_001301786.1
Accession Version	NM_001301786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	cadmium/zinc-transporting ATPase HMA2-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KJ818258.1. On Sep 12, 2014 this sequence version replaced XM_004146319.1. ##Evidence-Data-START## Transcript exon combination :: KJ818258.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02570546, SAMN02570552 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGA CGGAGGCAGA GGGAAATAAG GGTGGTGGGT TGAAGAAAAG TTACTTTGAT 
GTCTTGGGGA TTTGTTGTTC TTCAGAAATT CCGGTGATTG AGAATATATT GAAGGATATT 
GAAGGGATTA AAGAGATTAG AGTGATTGTT GCAACAAGAA CTGTCATTGT TCTTCATGAT 
GATCTTCTCG TTTCCCAAGC ACAAATTGTT AAAGCCCTGA ACCAAGCAAG ATTTGAAGCG 
AACGTTAGAG CGTACGGAGA TCAGAAAGAT CACCGGAAAA AATGGCCGAG CCCGTATGCG 
GTGGCCAGTG GGCTGTTGCT GCTCTTGTCG TTACTCAAAT ATGTGAATCC TATTTTTCGA 
TGGGTCGCTT TAGCCGCCGT CGCCGCCGGA ATCTTGCCCA TTGTCCTCAA AAGCTTCGCC 
GCCGTTCGCC ATCTCAGAAT CGACATCAAT ATTCTTGCTT TAATTGCAGT AATCGGAACA 
ATTGTGCTGA AGGATTACTT GGAAGCAGCC ACCATCGTCT TCCTCTTCAC CATCGCCGAA 
TGGCTCGAGT CCAGAGCCGC CCACAAGGCC AACGCCGTCA TGTCATCCCT TCTAAGTATT 
GCTCCTCAGA AAGCGGTTCT AGCCGACACT GGCGAAGTTG TCGGCGCCGA CGAGGTCAAA 
TTAGGGACTC TTCTGGCTGT GAAGGCCGGT GAGGACATAC CCATTGATGG AATCGTTGTG 
GAAGGAAAAT GTGAAGTGGA TGAGAAAACA CTTACAGGCG AATCTTTTCC TGTTCCCAAA 
CAAAAGAATT CCACTGTTTG GGCTGGTACC ATTAATCTAA ATGGCTATGT TACTGTGAAG 
ACTACTGCCC TTGCTGAAGA CTGTGTGGTG GCCAAAATGG CTAAGCTGGT TGAGGAGGCC 
CAGAACAATA AATCTAGAAC TCAAAGGTTC ATTGACAAAT GTGCCAAATT CTATACTCCA 
GCTGTTATAA TCATATCAAC TTGCATAGTA GTGATTCCAC TTGCTTTGAG ACTTCCCAAT 
CGCAGCCATT GGTTTCACTT GGCCTTGGTT GTGTTAGTAA GTGCGTGTCC CTGTGCACTC 
ATCCTTTCCA CTCCTGTTGC GTCTTTCTGT GCACTTACTA AGGCAGCAAC ATCTGGTCTT 
TTAATCAAAG GAGGTGACTA CCTTGAAACA CTTGGCAAAA TTAAGATCAT GGCCTTTGAT 
AAAACTGGGA CAATAACAAG AGGTGAATTT ATGGTGACTG AATTTCAAGT TCTTGATAAG 
GACAATATAA GCTTAGACAC ATTGCTGTAC TGGGTGTCGA GCATCGAGAG CAAGTCCAGT 
CATCCAATGG CAGCTGCACT TGTTGACCAT GGAAGATCAC TATCCATTGA TCCCAAACCT 
GAAAATGTGG ACGACTTTCA AAATTTTCCT GGAGAAGGTG TTCATGGGAG AATTGATGGG 
AAAGACATCT ATATTGGAAA TCGAAAAATT GCTACAAGAG CCAACTGCGC AACAGTCCCA 
GAGATCAAAG ATGAAGCAAA GGATGGGAGG ACTGTTGGAT ACATATTCTG TGGAACTATT 
GCCGCTGGAG TCTTTAGTCT GTCTGATTCG TGTAGAACAG GGGCTAAAGA GGCCATGGAT 
GAGCTTAGAT CTCTCGGTAT AAAAACAGCC ATGCTCACTG GAGACAGTTC TGCAGCAGCC 
TTGCAAGCAC AAAAAGAATT GGGAAAGGCT TTACAAGCAG TTCATGCCGA ACTTCTACCT 
CAAGACAAGA CAAGGCTTAT CAATGACTTC AAAAAGGAGG GGCCAACAGC CATGATTGGA 
GATGGTCTAA ACGATGCCCC TGCCTTGGCC ACAGCTGATA TTGGCATATC AATGGGAATC 
TCAGGTTCAG CCCTCGCAAT AGAAACTGGA GATGTAATTT TAATGACTAA TGATATCAGG 
AAAGTTCCAA AAGCCATTCG ACTTGCAAGA AGAGCTAATA ATAAAGTAAT TGAAAATGTG 
ATTCTATCCG TTGTTCCTAG GATTGCTATA CTTGGCCTGG CATTTGGTGG TCATCCACTT 
GTTTGGGCAG CAGTTCTTGC TGATGTTGGT GCCTGTGTGC TAGTTATCCT CAACAGTATG 
CTCCTGCTGC GAGGAACAGA CGGACACAAA GGGAAGAAAG CTGGCAAGTT TTCTGCTACT 
CATGGTTCCT CCAAACATAA ATGTTGTCAC GTTAGCAGCC ATTCAGATGA ATGTAGTGGT 
CACACCCATG ATCATGGTTG CAACCATCGC AGTTCTCACT CTTCTAGTCA TAGTCACCAT 
CACCATCACC ATCACCATGA GCACGAGGAC TGTGGCTCTC TCAAGAATAC TCATGATGGT 
TGTTTACAAA AGAATCATCC CTCCATGTGT GATTCCAAGT TGAAAAATTC AAGTTCATGC 
AAGAAAAGTA AACTCATGAA CCCAAGTTCT AAGATAGATG GTTCTACCGG GTGTGTAAAA 
CTCTGTGAAC ATGATCACAC CCATGATCAC GGGTGCAGTG ACGGCTCGGA CTCTTCTAGT 
CATAGTCATC ATCAACATCA CCACCACCAT CATCATGAGC ACGAGGATTG CCACTCGCTC 
GAGAAAACTC ATGATGTTTG TTTACCTCAG AATCATGCTT CCAAGTGTGA TTCCAGGTCG 
AAAAATTTGA GTTCGTTCAA CGAAGGTAAA CACATAGACT CATGTTCTAA GGTAGATGGT 
TCTACCGGTA GTGTACAACT CTGTGAACAT GATCACATGC ATGATCATGG GTGCAACACC 
GACAGTACTG ACTCTTCTAG TCATAGTCAC CACCACCATT ATGAGCACGA GGACTGTGGT 
TCTCTCAAGA AAACTCATGA TGGTTGCGTA CTTCAGAATT GTGCTTCAAA GTGTGATTCC 
GGGATGAAAT CTTCAAGTTC ATGCAAGAAA AGTAAACTCG TGGATTCAAG TTCTAAGGTA 
GATGATTCTG CAAGCAGTCT AAAACCCTGT GAACATGGAC ATATCTGTAA CGATCAGCCT 
GCTGAACACG ACCACCATCC TTATTCTTCA TGCACTGATC ATCATGCCAA GGATGAACTC 
TGCTCTCCAG AGAATACACA AGAGTTTTGC TCGTTTCAGA AGTGTGCATC AAATTCATGT 
GAGAAAATCA AGTGCACAAG CTCACCCGCA AGCCACGATG GATCTGCTGT GATTGTTGAG 
CTCGATGAAA GTGGATGTTG TACTCATAAT ACCCAATCTG CTCAACATGA TCATGACATC 
CAAAGTCCCA AATGTGATTT TGATGATAGC CACTCACCCA GTCTTGAACA TCATATTAGT 
AATGGTTGTT GCTCTCAAAA GAATACTCAC AAGGATTTTT TATCTCATCC AATGCGTGAT 
TCTGAAACCT GCAAGGAAGG CGTTCATCTT CATTGTAAAG CATCAAATGA AGATAATGGA 
GCAATCAACA ATACTGTAAA TATCAAGCTT GAAGCAGACC ATTCGAACTC TAAACGTGGC 
AACACTAGTA ATAAGCCAAT GGAAAACAGA GAGACAAACA ACTGTAAAAG CTGCAGAAGG 
GGAAGTTCAC AATTCAAAAT TGGAAAATCT TGTGCAGGCT TGAATAAGAG AGAAGTGGGT 
GGGTGTTGTA AGAGCTACAT GAAGGAGTGT TGTAGGAAGC ATGGCGATAT CAGAATGGCA 
GTTCGTGGAG GCTTAAACGA AATCATCATA GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288715.1
CDS	1..3696
Translation	MAATEAEGNKGGGLKKSYFDVLGICCSSEIPVIENILKDIEGIKEIRVIVATRTVIVLHDDLLVSQAQIVKALNQARFEANVRAYGDQKDHRKKWPSPYAVASGLLLLLSLLKYVNPIFRWVALAAVAAGILPIVLKSFAAVRHLRIDINILALIAVIGTIVLKDYLEAATIVFLFTIAEWLESRAAHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDKCAKFYTPAVIIISTCIVVIPLALRLPNRSHWFHLALVVLVSACPCALILSTPVASFCALTKAATSGLLIKGGDYLETLGKIKIMAFDKTGTITRGEFMVTEFQVLDKDNISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSIDPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRKIATRANCATVPEIKDEAKDGRTVGYIFCGTIAAGVFSLSDSCRTGAKEAMDELRSLGIKTAMLTGDSSAAALQAQKELGKALQAVHAELLPQDKTRLINDFKKEGPTAMIGDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKVPKAIRLARRANNKVIENVILSVVPRIAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTDGHKGKKAGKFSATHGSSKHKCCHVSSHSDECSGHTHDHGCNHRSSHSSSHSHHHHHHHHEHEDCGSLKNTHDGCLQKNHPSMCDSKLKNSSSCKKSKLMNPSSKIDGSTGCVKLCEHDHTHDHGCSDGSDSSSHSHHQHHHHHHHEHEDCHSLEKTHDVCLPQNHASKCDSRSKNLSSFNEGKHIDSCSKVDGSTGSVQLCEHDHMHDHGCNTDSTDSSSHSHHHHYEHEDCGSLKKTHDGCVLQNCASKCDSGMKSSSSCKKSKLVDSSSKVDDSASSLKPCEHGHICNDQPAEHDHHPYSSCTDHHAKDELCSPENTQEFCSFQKCASNSCEKIKCTSSPASHDGSAVIVELDESGCCTHNTQSAQHDHDIQSPKCDFDDSHSPSLEHHISNGCCSQKNTHKDFLSHPMRDSETCKEGVHLHCKASNEDNGAINNTVNIKLEADHSNSKRGNTSNKPMENRETNNCKSCRRGSSQFKIGKSCAGLNKREVGGCCKSYMKECCRKHGDIRMAVRGGLNEIIIE

Target ORF information:

RefSeq Version	NM_001301786.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus cadmium/zinc-transporting ATPase HMA2-like (HMA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301786.1

ORF Insert Sequence:

ATGGCGGCGA CGGAGGCAGA GGGAAATAAG GGTGGTGGGT TGAAGAAAAG TTACTTTGAT 
GTCTTGGGGA TTTGTTGTTC TTCAGAAATT CCGGTGATTG AGAATATATT GAAGGATATT 
GAAGGGATTA AAGAGATTAG AGTGATTGTT GCAACAAGAA CTGTCATTGT TCTTCATGAT 
GATCTTCTCG TTTCCCAAGC ACAAATTGTT AAAGCCCTGA ACCAAGCAAG ATTTGAAGCG 
AACGTTAGAG CGTACGGAGA TCAGAAAGAT CACCGGAAAA AATGGCCGAG CCCGTATGCG 
GTGGCCAGTG GGCTGTTGCT GCTCTTGTCG TTACTCAAAT ATGTGAATCC TATTTTTCGA 
TGGGTCGCTT TAGCCGCCGT CGCCGCCGGA ATCTTGCCCA TTGTCCTCAA AAGCTTCGCC 
GCCGTTCGCC ATCTCAGAAT CGACATCAAT ATTCTTGCTT TAATTGCAGT AATCGGAACA 
ATTGTGCTGA AGGATTACTT GGAAGCAGCC ACCATCGTCT TCCTCTTCAC CATCGCCGAA 
TGGCTCGAGT CCAGAGCCGC CCACAAGGCC AACGCCGTCA TGTCATCCCT TCTAAGTATT 
GCTCCTCAGA AAGCGGTTCT AGCCGACACT GGCGAAGTTG TCGGCGCCGA CGAGGTCAAA 
TTAGGGACTC TTCTGGCTGT GAAGGCCGGT GAGGACATAC CCATTGATGG AATCGTTGTG 
GAAGGAAAAT GTGAAGTGGA TGAGAAAACA CTTACAGGCG AATCTTTTCC TGTTCCCAAA 
CAAAAGAATT CCACTGTTTG GGCTGGTACC ATTAATCTAA ATGGCTATGT TACTGTGAAG 
ACTACTGCCC TTGCTGAAGA CTGTGTGGTG GCCAAAATGG CTAAGCTGGT TGAGGAGGCC 
CAGAACAATA AATCTAGAAC TCAAAGGTTC ATTGACAAAT GTGCCAAATT CTATACTCCA 
GCTGTTATAA TCATATCAAC TTGCATAGTA GTGATTCCAC TTGCTTTGAG ACTTCCCAAT 
CGCAGCCATT GGTTTCACTT GGCCTTGGTT GTGTTAGTAA GTGCGTGTCC CTGTGCACTC 
ATCCTTTCCA CTCCTGTTGC GTCTTTCTGT GCACTTACTA AGGCAGCAAC ATCTGGTCTT 
TTAATCAAAG GAGGTGACTA CCTTGAAACA CTTGGCAAAA TTAAGATCAT GGCCTTTGAT 
AAAACTGGGA CAATAACAAG AGGTGAATTT ATGGTGACTG AATTTCAAGT TCTTGATAAG 
GACAATATAA GCTTAGACAC ATTGCTGTAC TGGGTGTCGA GCATCGAGAG CAAGTCCAGT 
CATCCAATGG CAGCTGCACT TGTTGACCAT GGAAGATCAC TATCCATTGA TCCCAAACCT 
GAAAATGTGG ACGACTTTCA AAATTTTCCT GGAGAAGGTG TTCATGGGAG AATTGATGGG 
AAAGACATCT ATATTGGAAA TCGAAAAATT GCTACAAGAG CCAACTGCGC AACAGTCCCA 
GAGATCAAAG ATGAAGCAAA GGATGGGAGG ACTGTTGGAT ACATATTCTG TGGAACTATT 
GCCGCTGGAG TCTTTAGTCT GTCTGATTCG TGTAGAACAG GGGCTAAAGA GGCCATGGAT 
GAGCTTAGAT CTCTCGGTAT AAAAACAGCC ATGCTCACTG GAGACAGTTC TGCAGCAGCC 
TTGCAAGCAC AAAAAGAATT GGGAAAGGCT TTACAAGCAG TTCATGCCGA ACTTCTACCT 
CAAGACAAGA CAAGGCTTAT CAATGACTTC AAAAAGGAGG GGCCAACAGC CATGATTGGA 
GATGGTCTAA ACGATGCCCC TGCCTTGGCC ACAGCTGATA TTGGCATATC AATGGGAATC 
TCAGGTTCAG CCCTCGCAAT AGAAACTGGA GATGTAATTT TAATGACTAA TGATATCAGG 
AAAGTTCCAA AAGCCATTCG ACTTGCAAGA AGAGCTAATA ATAAAGTAAT TGAAAATGTG 
ATTCTATCCG TTGTTCCTAG GATTGCTATA CTTGGCCTGG CATTTGGTGG TCATCCACTT 
GTTTGGGCAG CAGTTCTTGC TGATGTTGGT GCCTGTGTGC TAGTTATCCT CAACAGTATG 
CTCCTGCTGC GAGGAACAGA CGGACACAAA GGGAAGAAAG CTGGCAAGTT TTCTGCTACT 
CATGGTTCCT CCAAACATAA ATGTTGTCAC GTTAGCAGCC ATTCAGATGA ATGTAGTGGT 
CACACCCATG ATCATGGTTG CAACCATCGC AGTTCTCACT CTTCTAGTCA TAGTCACCAT 
CACCATCACC ATCACCATGA GCACGAGGAC TGTGGCTCTC TCAAGAATAC TCATGATGGT 
TGTTTACAAA AGAATCATCC CTCCATGTGT GATTCCAAGT TGAAAAATTC AAGTTCATGC 
AAGAAAAGTA AACTCATGAA CCCAAGTTCT AAGATAGATG GTTCTACCGG GTGTGTAAAA 
CTCTGTGAAC ATGATCACAC CCATGATCAC GGGTGCAGTG ACGGCTCGGA CTCTTCTAGT 
CATAGTCATC ATCAACATCA CCACCACCAT CATCATGAGC ACGAGGATTG CCACTCGCTC 
GAGAAAACTC ATGATGTTTG TTTACCTCAG AATCATGCTT CCAAGTGTGA TTCCAGGTCG 
AAAAATTTGA GTTCGTTCAA CGAAGGTAAA CACATAGACT CATGTTCTAA GGTAGATGGT 
TCTACCGGTA GTGTACAACT CTGTGAACAT GATCACATGC ATGATCATGG GTGCAACACC 
GACAGTACTG ACTCTTCTAG TCATAGTCAC CACCACCATT ATGAGCACGA GGACTGTGGT 
TCTCTCAAGA AAACTCATGA TGGTTGCGTA CTTCAGAATT GTGCTTCAAA GTGTGATTCC 
GGGATGAAAT CTTCAAGTTC ATGCAAGAAA AGTAAACTCG TGGATTCAAG TTCTAAGGTA 
GATGATTCTG CAAGCAGTCT AAAACCCTGT GAACATGGAC ATATCTGTAA CGATCAGCCT 
GCTGAACACG ACCACCATCC TTATTCTTCA TGCACTGATC ATCATGCCAA GGATGAACTC 
TGCTCTCCAG AGAATACACA AGAGTTTTGC TCGTTTCAGA AGTGTGCATC AAATTCATGT 
GAGAAAATCA AGTGCACAAG CTCACCCGCA AGCCACGATG GATCTGCTGT GATTGTTGAG 
CTCGATGAAA GTGGATGTTG TACTCATAAT ACCCAATCTG CTCAACATGA TCATGACATC 
CAAAGTCCCA AATGTGATTT TGATGATAGC CACTCACCCA GTCTTGAACA TCATATTAGT 
AATGGTTGTT GCTCTCAAAA GAATACTCAC AAGGATTTTT TATCTCATCC AATGCGTGAT 
TCTGAAACCT GCAAGGAAGG CGTTCATCTT CATTGTAAAG CATCAAATGA AGATAATGGA 
GCAATCAACA ATACTGTAAA TATCAAGCTT GAAGCAGACC ATTCGAACTC TAAACGTGGC 
AACACTAGTA ATAAGCCAAT GGAAAACAGA GAGACAAACA ACTGTAAAAG CTGCAGAAGG 
GGAAGTTCAC AATTCAAAAT TGGAAAATCT TGTGCAGGCT TGAATAAGAG AGAAGTGGGT 
GGGTGTTGTA AGAGCTACAT GAAGGAGTGT TGTAGGAAGC ATGGCGATAT CAGAATGGCA 
GTTCGTGGAG GCTTAAACGA AATCATCATA GAATAG