LOC101203251 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101203251
Entrez Gene ID	101203251
Full Name	mitogen-activated protein kinase kinase kinase NPK1
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004145265.2	XP_004145313.2	mitogen-activated protein kinase kinase kinase NPK1
XM_004145265.3	XP_004145313.2	mitogen-activated protein kinase kinase kinase 18

Pathways from BioSystems
Gene Ontology

The following LOC101203251 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101203251 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu54386	XM_004145265.3 Latest version!	Cucumis sativus mitogen-activated protein kinase kinase kinase 18 (LOC101203251), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCu54386	XM_004145265.2	Cucumis sativus mitogen-activated protein kinase kinase kinase NPK1 (LOC101203251), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101203251 ( XM_004145265.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004145313.2 Cucumis sativus mitogen-activated protein kinase kinase kinase NPK1 (LOC101203251), mRNA.

Price & Availability↑

CloneID	OCu54386
Clone ID Related Accession (Same CDS sequence)	XM_004145265.2 , XM_004145265.3
Accession Version	XM_004145265.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	mitogen-activated protein kinase kinase kinase NPK1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 23, 2015 this sequence version replaced XM_004145265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA CCACCGCGCT TTCTTGGACT CGAAGTAATT CTCCCATCGG AAAAGGCTCC 
TTTGCCACCG TCTCTCTCGG AATTCGAAAA CCCGACGCTC GGATTTTCGC CGTCAAATCT 
GTCCAGCAGA CTCAGACACT CCGCCCACAA ATCGACTGCT TAGAGAATGA AATTCGAATC 
CTCCGATCCT TGAACTCGCC TTACGTGGTT GCCTTTCTCG GCGATGATGT TTCCCATGAA 
TCTCCCACCA CCTCCTTTCG TAACCTTCAT ATGGAGTATT TGCCAGGTGG CACCGCAGCA 
GATGACCCCA CTGGCACTCG CGATGATAAG CTTTTACGGG AGAGGACATG GTGCCTCGTT 
TCGGCTTTGA GTTACATTCA TTCCAAAGGA ATTGTTCACT GTGATGTTAA AGGGAGGAAC 
GTTTTGATCG GATTGAATCC CGGGTTTCTG AAATTGGCTG ATTTCGGCTC TGCAATTGAA 
CTTCACGGCC CCGGCCACAG GTCTCGGGAT TCGCTTGCGC CACGTGGGAG TCCACTATGG 
ATGGCGCCAG AGGTAGTCCG CGGAGAATTT CAGGGCCCGG AATCGGACGT TTGGTCCCTT 
GGGTGCACGG TCATTGAAAT GGTCACCGGG AAACCCGCAT GGGAAGATTT CGGAGCCGAT 
ACGCTCAGTC GAATCGGCTT TTCCGACGAT TTGCCAGACT TTCCAACATG CTTATCGGAA 
GTCTGCCGGG ATTTTCTTCG GAAGTGTCTC AGAAGAAATC CTAGTGAACG GTGGAGCTGT 
GATCGGCTGC TGCAGCATCC ATTTCTGGCG GCGGCGGCGG CGGCGGCGGC CTCGCCGAAG 
ATTGCTGTAG AGAATTCCCC CAGGTGCGTT CTTGACTGGG TCAACGTCAG TTTCTCCGAC 
GACGAGGAGG AAATTCCACA CGCCGACGAA GCCTCCGGAT CCGGCGGCCA GGAAAATGAG 
ATATATGGTA AGGAAAGAAT TGGGAAATTG AGTACTACGA GTGAATGGCC AAATTGGGAA 
TCAGACGGTT GGTCGGCGGT GAGAAGTAGT TACAGTGAAG CGGCGGCGGA GACAGAGGCG 
AGCTGCCGGA AAGAAGAAGA AGAAGAAGAA GGCGGAGGGG CAGAATGGGA ATGTGGAAAT 
TTGAGAAGGG TAGAAGGGGA AATGGAAGGG AGAAGTTGGG AATATTCGGA GTTTGTAAGA 
AGAGACAATC ACGGAAAATT GGGGGCCGAA TATTCAAATC CTGGCGGTAT GATCATTCCG 
GAGCGGCCAC GTAATAATTT CGGCGGTGGT GGCGGTTGCG GTAGCGGTGG ATTAAGTTTC 
CGGCGGTTGG GATATGAAAT ATCTGAAATT ACAACAATAA TAACCTCATG GATATACTCA 
ATTGAATTGA TATTATGTTG TTACTATTGG AATATATTAA TGAAGAAATT GGTGTTATTT 
GGGAATTACA CTTTCTTACC TTTCTTTTCT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004145313.2
CDS	82..1557
Translation	MLKTTALSWTRSNSPIGKGSFATVSLGIRKPDARIFAVKSVQQTQTLRPQIDCLENEIRILRSLNSPYVVAFLGDDVSHESPTTSFRNLHMEYLPGGTAADDPTGTRDDKLLRERTWCLVSALSYIHSKGIVHCDVKGRNVLIGLNPGFLKLADFGSAIELHGPGHRSRDSLAPRGSPLWMAPEVVRGEFQGPESDVWSLGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPDFPTCLSEVCRDFLRKCLRRNPSERWSCDRLLQHPFLAAAAAAAASPKIAVENSPRCVLDWVNVSFSDDEEEIPHADEASGSGGQENEIYGKERIGKLSTTSEWPNWESDGWSAVRSSYSEAAAETEASCRKEEEEEEGGGAEWECGNLRRVEGEMEGRSWEYSEFVRRDNHGKLGAEYSNPGGMIIPERPRNNFGGGGGCGSGGLSFRRLGYEISEITTIITSWIYSIELILCCYYWNILMKKLVLFGNYTFLPFFSA

Target ORF information:

RefSeq Version	XM_004145265.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus mitogen-activated protein kinase kinase kinase NPK1 (LOC101203251), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004145265.2

ORF Insert Sequence:

ATGCTCAAAA CCACCGCGCT TTCTTGGACT CGAAGTAATT CTCCCATCGG AAAAGGCTCC 
TTTGCCACCG TCTCTCTCGG AATTCGAAAA CCCGACGCTC GGATTTTCGC CGTCAAATCT 
GTCCAGCAGA CTCAGACACT CCGCCCACAA ATCGACTGCT TAGAGAATGA AATTCGAATC 
CTCCGATCCT TGAACTCGCC TTACGTGGTT GCCTTTCTCG GCGATGATGT TTCCCATGAA 
TCTCCCACCA CCTCCTTTCG TAACCTTCAT ATGGAGTATT TGCCAGGTGG CACCGCAGCA 
GATGACCCCA CTGGCACTCG CGATGATAAG CTTTTACGGG AGAGGACATG GTGCCTCGTT 
TCGGCTTTGA GTTACATTCA TTCCAAAGGA ATTGTTCACT GTGATGTTAA AGGGAGGAAC 
GTTTTGATCG GATTGAATCC CGGGTTTCTG AAATTGGCTG ATTTCGGCTC TGCAATTGAA 
CTTCACGGCC CCGGCCACAG GTCTCGGGAT TCGCTTGCGC CACGTGGGAG TCCACTATGG 
ATGGCGCCAG AGGTAGTCCG CGGAGAATTT CAGGGCCCGG AATCGGACGT TTGGTCCCTT 
GGGTGCACGG TCATTGAAAT GGTCACCGGG AAACCCGCAT GGGAAGATTT CGGAGCCGAT 
ACGCTCAGTC GAATCGGCTT TTCCGACGAT TTGCCAGACT TTCCAACATG CTTATCGGAA 
GTCTGCCGGG ATTTTCTTCG GAAGTGTCTC AGAAGAAATC CTAGTGAACG GTGGAGCTGT 
GATCGGCTGC TGCAGCATCC ATTTCTGGCG GCGGCGGCGG CGGCGGCGGC CTCGCCGAAG 
ATTGCTGTAG AGAATTCCCC CAGGTGCGTT CTTGACTGGG TCAACGTCAG TTTCTCCGAC 
GACGAGGAGG AAATTCCACA CGCCGACGAA GCCTCCGGAT CCGGCGGCCA GGAAAATGAG 
ATATATGGTA AGGAAAGAAT TGGGAAATTG AGTACTACGA GTGAATGGCC AAATTGGGAA 
TCAGACGGTT GGTCGGCGGT GAGAAGTAGT TACAGTGAAG CGGCGGCGGA GACAGAGGCG 
AGCTGCCGGA AAGAAGAAGA AGAAGAAGAA GGCGGAGGGG CAGAATGGGA ATGTGGAAAT 
TTGAGAAGGG TAGAAGGGGA AATGGAAGGG AGAAGTTGGG AATATTCGGA GTTTGTAAGA 
AGAGACAATC ACGGAAAATT GGGGGCCGAA TATTCAAATC CTGGCGGTAT GATCATTCCG 
GAGCGGCCAC GTAATAATTT CGGCGGTGGT GGCGGTTGCG GTAGCGGTGG ATTAAGTTTC 
CGGCGGTTGG GATATGAAAT ATCTGAAATT ACAACAATAA TAACCTCATG GATATACTCA 
ATTGAATTGA TATTATGTTG TTACTATTGG AATATATTAA TGAAGAAATT GGTGTTATTT 
GGGAATTACA CTTTCTTACC TTTCTTTTCT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101203251 ( XM_004145265.3 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004145313.2 Cucumis sativus mitogen-activated protein kinase kinase kinase 18 (LOC101203251), mRNA.

Price & Availability↑

CloneID	OCu54386
Clone ID Related Accession (Same CDS sequence)	XM_004145265.2 , XM_004145265.3
Accession Version	XM_004145265.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	mitogen-activated protein kinase kinase kinase 18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026655.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_004145265.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA CCACCGCGCT TTCTTGGACT CGAAGTAATT CTCCCATCGG AAAAGGCTCC 
TTTGCCACCG TCTCTCTCGG AATTCGAAAA CCCGACGCTC GGATTTTCGC CGTCAAATCT 
GTCCAGCAGA CTCAGACACT CCGCCCACAA ATCGACTGCT TAGAGAATGA AATTCGAATC 
CTCCGATCCT TGAACTCGCC TTACGTGGTT GCCTTTCTCG GCGATGATGT TTCCCATGAA 
TCTCCCACCA CCTCCTTTCG TAACCTTCAT ATGGAGTATT TGCCAGGTGG CACCGCAGCA 
GATGACCCCA CTGGCACTCG CGATGATAAG CTTTTACGGG AGAGGACATG GTGCCTCGTT 
TCGGCTTTGA GTTACATTCA TTCCAAAGGA ATTGTTCACT GTGATGTTAA AGGGAGGAAC 
GTTTTGATCG GATTGAATCC CGGGTTTCTG AAATTGGCTG ATTTCGGCTC TGCAATTGAA 
CTTCACGGCC CCGGCCACAG GTCTCGGGAT TCGCTTGCGC CACGTGGGAG TCCACTATGG 
ATGGCGCCAG AGGTAGTCCG CGGAGAATTT CAGGGCCCGG AATCGGACGT TTGGTCCCTT 
GGGTGCACGG TCATTGAAAT GGTCACCGGG AAACCCGCAT GGGAAGATTT CGGAGCCGAT 
ACGCTCAGTC GAATCGGCTT TTCCGACGAT TTGCCAGACT TTCCAACATG CTTATCGGAA 
GTCTGCCGGG ATTTTCTTCG GAAGTGTCTC AGAAGAAATC CTAGTGAACG GTGGAGCTGT 
GATCGGCTGC TGCAGCATCC ATTTCTGGCG GCGGCGGCGG CGGCGGCGGC CTCGCCGAAG 
ATTGCTGTAG AGAATTCCCC CAGGTGCGTT CTTGACTGGG TCAACGTCAG TTTCTCCGAC 
GACGAGGAGG AAATTCCACA CGCCGACGAA GCCTCCGGAT CCGGCGGCCA GGAAAATGAG 
ATATATGGTA AGGAAAGAAT TGGGAAATTG AGTACTACGA GTGAATGGCC AAATTGGGAA 
TCAGACGGTT GGTCGGCGGT GAGAAGTAGT TACAGTGAAG CGGCGGCGGA GACAGAGGCG 
AGCTGCCGGA AAGAAGAAGA AGAAGAAGAA GGCGGAGGGG CAGAATGGGA ATGTGGAAAT 
TTGAGAAGGG TAGAAGGGGA AATGGAAGGG AGAAGTTGGG AATATTCGGA GTTTGTAAGA 
AGAGACAATC ACGGAAAATT GGGGGCCGAA TATTCAAATC CTGGCGGTAT GATCATTCCG 
GAGCGGCCAC GTAATAATTT CGGCGGTGGT GGCGGTTGCG GTAGCGGTGG ATTAAGTTTC 
CGGCGGTTGG GATATGAAAT ATCTGAAATT ACAACAATAA TAACCTCATG GATATACTCA 
ATTGAATTGA TATTATGTTG TTACTATTGG AATATATTAA TGAAGAAATT GGTGTTATTT 
GGGAATTACA CTTTCTTACC TTTCTTTTCT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004145313.2
CDS	178..1653
Translation	MLKTTALSWTRSNSPIGKGSFATVSLGIRKPDARIFAVKSVQQTQTLRPQIDCLENEIRILRSLNSPYVVAFLGDDVSHESPTTSFRNLHMEYLPGGTAADDPTGTRDDKLLRERTWCLVSALSYIHSKGIVHCDVKGRNVLIGLNPGFLKLADFGSAIELHGPGHRSRDSLAPRGSPLWMAPEVVRGEFQGPESDVWSLGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPDFPTCLSEVCRDFLRKCLRRNPSERWSCDRLLQHPFLAAAAAAAASPKIAVENSPRCVLDWVNVSFSDDEEEIPHADEASGSGGQENEIYGKERIGKLSTTSEWPNWESDGWSAVRSSYSEAAAETEASCRKEEEEEEGGGAEWECGNLRRVEGEMEGRSWEYSEFVRRDNHGKLGAEYSNPGGMIIPERPRNNFGGGGGCGSGGLSFRRLGYEISEITTIITSWIYSIELILCCYYWNILMKKLVLFGNYTFLPFFSA

Target ORF information:

RefSeq Version	XM_004145265.3
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus mitogen-activated protein kinase kinase kinase 18 (LOC101203251), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004145265.3

ORF Insert Sequence:

ATGCTCAAAA CCACCGCGCT TTCTTGGACT CGAAGTAATT CTCCCATCGG AAAAGGCTCC 
TTTGCCACCG TCTCTCTCGG AATTCGAAAA CCCGACGCTC GGATTTTCGC CGTCAAATCT 
GTCCAGCAGA CTCAGACACT CCGCCCACAA ATCGACTGCT TAGAGAATGA AATTCGAATC 
CTCCGATCCT TGAACTCGCC TTACGTGGTT GCCTTTCTCG GCGATGATGT TTCCCATGAA 
TCTCCCACCA CCTCCTTTCG TAACCTTCAT ATGGAGTATT TGCCAGGTGG CACCGCAGCA 
GATGACCCCA CTGGCACTCG CGATGATAAG CTTTTACGGG AGAGGACATG GTGCCTCGTT 
TCGGCTTTGA GTTACATTCA TTCCAAAGGA ATTGTTCACT GTGATGTTAA AGGGAGGAAC 
GTTTTGATCG GATTGAATCC CGGGTTTCTG AAATTGGCTG ATTTCGGCTC TGCAATTGAA 
CTTCACGGCC CCGGCCACAG GTCTCGGGAT TCGCTTGCGC CACGTGGGAG TCCACTATGG 
ATGGCGCCAG AGGTAGTCCG CGGAGAATTT CAGGGCCCGG AATCGGACGT TTGGTCCCTT 
GGGTGCACGG TCATTGAAAT GGTCACCGGG AAACCCGCAT GGGAAGATTT CGGAGCCGAT 
ACGCTCAGTC GAATCGGCTT TTCCGACGAT TTGCCAGACT TTCCAACATG CTTATCGGAA 
GTCTGCCGGG ATTTTCTTCG GAAGTGTCTC AGAAGAAATC CTAGTGAACG GTGGAGCTGT 
GATCGGCTGC TGCAGCATCC ATTTCTGGCG GCGGCGGCGG CGGCGGCGGC CTCGCCGAAG 
ATTGCTGTAG AGAATTCCCC CAGGTGCGTT CTTGACTGGG TCAACGTCAG TTTCTCCGAC 
GACGAGGAGG AAATTCCACA CGCCGACGAA GCCTCCGGAT CCGGCGGCCA GGAAAATGAG 
ATATATGGTA AGGAAAGAAT TGGGAAATTG AGTACTACGA GTGAATGGCC AAATTGGGAA 
TCAGACGGTT GGTCGGCGGT GAGAAGTAGT TACAGTGAAG CGGCGGCGGA GACAGAGGCG 
AGCTGCCGGA AAGAAGAAGA AGAAGAAGAA GGCGGAGGGG CAGAATGGGA ATGTGGAAAT 
TTGAGAAGGG TAGAAGGGGA AATGGAAGGG AGAAGTTGGG AATATTCGGA GTTTGTAAGA 
AGAGACAATC ACGGAAAATT GGGGGCCGAA TATTCAAATC CTGGCGGTAT GATCATTCCG 
GAGCGGCCAC GTAATAATTT CGGCGGTGGT GGCGGTTGCG GTAGCGGTGG ATTAAGTTTC 
CGGCGGTTGG GATATGAAAT ATCTGAAATT ACAACAATAA TAACCTCATG GATATACTCA 
ATTGAATTGA TATTATGTTG TTACTATTGG AATATATTAA TGAAGAAATT GGTGTTATTT 
GGGAATTACA CTTTCTTACC TTTCTTTTCT GCCTAA