mapkk3/6 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	mapkk3/6
Entrez Gene ID	778902
Full Name	mitogen-activated protein kinase kinase
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001078473.2	NP_001071941.1	mitogen-activated protein kinase kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.
Imai KS, Hino K, Yagi K, Satoh N, Satou Y
Development (Cambridge, England)131(16)4047-58(2004 Aug)

Genomewide surveys of developmentally relevant genes in Ciona intestinalis.
Satou Y, Satoh N
Development genes and evolution213(5-6)211-2(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mapkk3/6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mapkk3/6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi13609	NM_001078473.2 Latest version!	Ciona intestinalis mitogen-activated protein kinase kinase (mapkk3/6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mapkk3/6 ( NM_001078473.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> NP_001071941.1 Ciona intestinalis mitogen-activated protein kinase kinase (mapkk3/6), mRNA.

Price & Availability↑

CloneID	OCi13609
Clone ID Related Accession (Same CDS sequence)	NM_001078473.2
Accession Version	NM_001078473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Ciona intestinalis(vase tunicate)
Product	mitogen-activated protein kinase kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB210540.1. On Aug 31, 2012 this sequence version replaced NM_001078473.1. ##Evidence-Data-START## Transcript exon combination :: AB210540.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00013799, SAMD00028078 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACTC ATGGAAATGG TCCCAATAAA TCTACGGATT CGGGACCAGC ACTACCAGTT 
CGAAGAAGAA AAAAAAACAA CAAATTAAAA GTTCGTATTG ATCCTCCCGA GCAAACCCCA 
AAAGTTCCGA TCGTTCCGCA CAAACCACAA TCGAGGATGA ACATAAATGG GCAAGACATC 
GATATTCGCG CTGATGATCT GACTATAGTT GAAGAGTTAG GACGAGGGGC GTATGGGGTC 
GTCGATAAGA TGAAACACAA ATCTACCGAA ACGATAATCG CGGTTAAACG GATCCGTGCC 
ACCTTGAACA CACAAGAACA GAAACGACTT CTGATGGATC TCGATGTCTC GATGAGATCG 
GTTGATTGTG ATTATACAGT CACTTTCTAC GGGGCGCTTT TCCGACAAGG AGACGTTTGG 
ATTTGCATGG AAGTGATGGA CACCTCTCTC GATCAGTTTT ATAAAAAGGT GAAGGACCGT 
GGTTTAAAGA CGGGTGAGGA CGTGTTGGGA ATAATAGCGG TGTCCATCGT TAAAGCACTT 
CGGTATTTAC AAGAAACTCT CCACGTCATT CATCGGGATG TTAAACCTTC CAATGTATTG 
TTGAACAAGA ATGGCCAGGT AAAGTTGTGC GACTTTGGAA TTAGCGGACA ACTCGTCGAC 
TCGCTCGTCA AAACCATTGA CGCTGGATGC AAACCTTATA TGGCGCCCGA ACGCATCGAC 
CCTGCTCAAA ACCAACAAGG ATATGACGTC AAGAGTGACG TATGGAGTCT TGGGATTACC 
ATGATAGAAG TCGCCACCGG GGATTTCCCC TACGACACCT GGCGGAATCC ATTTCAACAA 
TTAAAACAGG TAGTAGAAGA TCCTTCCCCC AAACTTCCAG CCAACACCTT CTCGCTCGAA 
CTCGACGACT TTTGTCGATG TTGTTTAAAC AAAGATCCGT CGAAACGCCC AAACTACAGG 
GAACTTGAAG ATCATGTGTT TGTACAGAAA CATCTTCATG AGGACGTTCG CAGTATTTCT 
TCTTTCATAA CTCTTATTCT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071941.1
CDS	52..1098
Translation	MATHGNGPNKSTDSGPALPVRRRKKNNKLKVRIDPPEQTPKVPIVPHKPQSRMNINGQDIDIRADDLTIVEELGRGAYGVVDKMKHKSTETIIAVKRIRATLNTQEQKRLLMDLDVSMRSVDCDYTVTFYGALFRQGDVWICMEVMDTSLDQFYKKVKDRGLKTGEDVLGIIAVSIVKALRYLQETLHVIHRDVKPSNVLLNKNGQVKLCDFGISGQLVDSLVKTIDAGCKPYMAPERIDPAQNQQGYDVKSDVWSLGITMIEVATGDFPYDTWRNPFQQLKQVVEDPSPKLPANTFSLELDDFCRCCLNKDPSKRPNYRELEDHVFVQKHLHEDVRSISSFITLILE

Target ORF information:

RefSeq Version	NM_001078473.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis mitogen-activated protein kinase kinase (mapkk3/6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078473.2

ORF Insert Sequence:

ATGGCAACTC ATGGAAATGG TCCCAATAAA TCTACGGATT CGGGACCAGC ACTACCAGTT 
CGAAGAAGAA AAAAAAACAA CAAATTAAAA GTTCGTATTG ATCCTCCCGA GCAAACCCCA 
AAAGTTCCGA TCGTTCCGCA CAAACCACAA TCGAGGATGA ACATAAATGG GCAAGACATC 
GATATTCGCG CTGATGATCT GACTATAGTT GAAGAGTTAG GACGAGGGGC GTATGGGGTC 
GTCGATAAGA TGAAACACAA ATCTACCGAA ACGATAATCG CGGTTAAACG GATCCGTGCC 
ACCTTGAACA CACAAGAACA GAAACGACTT CTGATGGATC TCGATGTCTC GATGAGATCG 
GTTGATTGTG ATTATACAGT CACTTTCTAC GGGGCGCTTT TCCGACAAGG AGACGTTTGG 
ATTTGCATGG AAGTGATGGA CACCTCTCTC GATCAGTTTT ATAAAAAGGT GAAGGACCGT 
GGTTTAAAGA CGGGTGAGGA CGTGTTGGGA ATAATAGCGG TGTCCATCGT TAAAGCACTT 
CGGTATTTAC AAGAAACTCT CCACGTCATT CATCGGGATG TTAAACCTTC CAATGTATTG 
TTGAACAAGA ATGGCCAGGT AAAGTTGTGC GACTTTGGAA TTAGCGGACA ACTCGTCGAC 
TCGCTCGTCA AAACCATTGA CGCTGGATGC AAACCTTATA TGGCGCCCGA ACGCATCGAC 
CCTGCTCAAA ACCAACAAGG ATATGACGTC AAGAGTGACG TATGGAGTCT TGGGATTACC 
ATGATAGAAG TCGCCACCGG GGATTTCCCC TACGACACCT GGCGGAATCC ATTTCAACAA 
TTAAAACAGG TAGTAGAAGA TCCTTCCCCC AAACTTCCAG CCAACACCTT CTCGCTCGAA 
CTCGACGACT TTTGTCGATG TTGTTTAAAC AAAGATCCGT CGAAACGCCC AAACTACAGG 
GAACTTGAAG ATCATGTGTT TGTACAGAAA CATCTTCATG AGGACGTTCG CAGTATTTCT 
TCTTTCATAA CTCTTATTCT TGAGTGA