fgf8/17/18 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	fgf8/17/18
Entrez Gene ID	445650
Full Name	fibroblast growth factor 8/17/18
Synonyms	Fgf18
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032476.1	NP_001027648.1	fibroblast growth factor 8/17/18

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis.
Racioppi C, Kamal AK, Razy-Krajka F, Gambardella G, Zanetti L, di Bernardo D, Sanges R, Christiaen LA, Ristoratore F
Nature communications54830(2014 Sep)

Neural tube patterning by Ephrin, FGF and Notch signaling relays.
Stolfi A, Wagner E, Taliaferro JM, Chou S, Levine M
Development (Cambridge, England)138(24)5429-39(2011 Dec)

FGF8/17/18 functions together with FGF9/16/20 during formation of the notochord in Ciona embryos.
Yasuo H, Hudson C
Developmental biology302(1)92-103(2007 Feb)

An integrated database of the ascidian, Ciona intestinalis: towards functional genomics.
Satou Y, Kawashima T, Shoguchi E, Nakayama A, Satoh N
Zoological science22(8)837-43(2005 Aug)

Region specific gene expressions in the central nervous system of the ascidian embryo.
Imai KS, Satoh N, Satou Y
Mechanisms of development119 Suppl 1S275-7(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis.
Title: Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis.

It was shown that the developing motor ganglion is patterned by sequential Ephrin/FGF8/MAPK and Delta/Notch signaling events. FGF8/MAPK attenuation by a localized Ephrin Ab signal specified posterior neuronal subtypes.
Title: Neural tube patterning by Ephrin, FGF and Notch signaling relays.

Study demonstrates that FGF9/16/20 acts solely during the initial induction step of notochord formation and that, subsequently, FGF8/17/18 together with FGF9/16/20 is involved in the following maintenance step.
Title: FGF8/17/18 functions together with FGF9/16/20 during formation of the notochord in Ciona embryos.

The following fgf8/17/18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fgf8/17/18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi13309	NM_001032476.1 Latest version!	Ciona intestinalis fibroblast growth factor 8/17/18 (fgf8/17/18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fgf8/17/18 ( NM_001032476.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> NP_001027648.1 Ciona intestinalis fibroblast growth factor 8/17/18 (fgf8/17/18), mRNA.

Price & Availability↑

CloneID	OCi13309
Clone ID Related Accession (Same CDS sequence)	NM_001032476.1
Accession Version	NM_001032476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Ciona intestinalis(vase tunicate)
Product	fibroblast growth factor 8/17/18
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB086096.1. ##Evidence-Data-START## Transcript exon combination :: AB086096.1, AK173762.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028078, SAMD00028079 [ECO:0000350] ##Evidence-Data-END##

ATGCGACCCT CTATTTGCAA AGATATCTAC GCGAACCTGT ACAGGTTCTT GCTGGGTATC 
ATCGTCCTCG GAGACATAGC CAGCGGGATT CCGCCAAATT ACATCAGAGA TTACATTAAA 
AGCGAAATAT TTCAAGAGAA CGTAAGCCAA TCTTCTTCCA CGAGAAGATA CTGTGAGATT 
TACTCGAAAC GAACCTCGAA CCACATTCGA ATACTCGGCA AGAAAGTGGA CGGGAGAGGA 
AGAAGAGGCA GCAATCATGC AAAACTAATA ATCCACTCTA AAGGCTTATG GGGTCGGATA 
ATGATTCAAG GCGTCCAAAC TGAACGGTTT CTTTGCATGA ATAAAAGAGG ACGACTAGTT 
GGAAAGAATT ATCGAAAAGC GATCAACGAT CCGAAGTGCC TATTCTTCGA AGAGATGTTG 
GAAAATTATT TTGATGCTTT TTCAAACTCT AAGTTTCGCG ATTGGCATAT TGGTTTCCAT 
TCAAACGGCA GACCAAAGCG AGGTCCACAC ACCAAAGAAA TCGGCACTGA TGAAATGTTC 
ATAAAGAAGT GTACAGCATC CAGCGGTATG GTACCAAACC CGACCGCTGC CAGTAACGAG 
AAGTACAAAT GGATTATTGG CGAATTCGAA AGGGACGTGA AGCGACATAA CAAGAAGAAA 
AAGAGAGCCC CTTTATCTGT TTTAAGACCA AATTATTCTG TCACTAGAGA TTCATCAACC 
GCCGACGAAG TTATGCTCGC AGACTCTTCC AGAACTTTGT CGACCCTACC TCCGTATGTT 
CAAGCTGAAA TCGAACGACG ACGGAGTCTG TACCCTGTTT TGCGACAGCA GCGTAGACTT 
GACGTTACCA TACCGCCAGC AGGTGGGACA GTGTCCGCCA TGGATCCGCT GTACTCGAGC 
CCAAAGCATC GAAAGTTACC TCGCCGCAAT AAACCTAGAC GGAGCAGACA AAATAGGAAA 
CGAAAACACA GAAGACATAA ATCGAAATGG AGGTCTACTG TTCCTACTGA TATAGTGGAT 
TCAGACTTTC AACCTACGAC TGGGACCTTT ACAACACCGT TCCCAATAAA CTCTGTAAGC 
GAACCCGAAG CCGGGTTTAT TAGAACCCCG ACTACAGCAC CGGGCCTGCA TAGACAACGA 
TCGCGGAGCG GGAGGCGGCG ACCGAAACAG AGAAGACGGA CGAAAAAAGT CCGTGGTCGG 
AAACAGAAAA CCGGTCGGCG GAACCGAAAA CGAGCAAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027648.1
CDS	98..1342
Translation	MRPSICKDIYANLYRFLLGIIVLGDIASGIPPNYIRDYIKSEIFQENVSQSSSTRRYCEIYSKRTSNHIRILGKKVDGRGRRGSNHAKLIIHSKGLWGRIMIQGVQTERFLCMNKRGRLVGKNYRKAINDPKCLFFEEMLENYFDAFSNSKFRDWHIGFHSNGRPKRGPHTKEIGTDEMFIKKCTASSGMVPNPTAASNEKYKWIIGEFERDVKRHNKKKKRAPLSVLRPNYSVTRDSSTADEVMLADSSRTLSTLPPYVQAEIERRRSLYPVLRQQRRLDVTIPPAGGTVSAMDPLYSSPKHRKLPRRNKPRRSRQNRKRKHRRHKSKWRSTVPTDIVDSDFQPTTGTFTTPFPINSVSEPEAGFIRTPTTAPGLHRQRSRSGRRRPKQRRRTKKVRGRKQKTGRRNRKRANT

Target ORF information:

RefSeq Version	NM_001032476.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis fibroblast growth factor 8/17/18 (fgf8/17/18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032476.1

ORF Insert Sequence:

ATGCGACCCT CTATTTGCAA AGATATCTAC GCGAACCTGT ACAGGTTCTT GCTGGGTATC 
ATCGTCCTCG GAGACATAGC CAGCGGGATT CCGCCAAATT ACATCAGAGA TTACATTAAA 
AGCGAAATAT TTCAAGAGAA CGTAAGCCAA TCTTCTTCCA CGAGAAGATA CTGTGAGATT 
TACTCGAAAC GAACCTCGAA CCACATTCGA ATACTCGGCA AGAAAGTGGA CGGGAGAGGA 
AGAAGAGGCA GCAATCATGC AAAACTAATA ATCCACTCTA AAGGCTTATG GGGTCGGATA 
ATGATTCAAG GCGTCCAAAC TGAACGGTTT CTTTGCATGA ATAAAAGAGG ACGACTAGTT 
GGAAAGAATT ATCGAAAAGC GATCAACGAT CCGAAGTGCC TATTCTTCGA AGAGATGTTG 
GAAAATTATT TTGATGCTTT TTCAAACTCT AAGTTTCGCG ATTGGCATAT TGGTTTCCAT 
TCAAACGGCA GACCAAAGCG AGGTCCACAC ACCAAAGAAA TCGGCACTGA TGAAATGTTC 
ATAAAGAAGT GTACAGCATC CAGCGGTATG GTACCAAACC CGACCGCTGC CAGTAACGAG 
AAGTACAAAT GGATTATTGG CGAATTCGAA AGGGACGTGA AGCGACATAA CAAGAAGAAA 
AAGAGAGCCC CTTTATCTGT TTTAAGACCA AATTATTCTG TCACTAGAGA TTCATCAACC 
GCCGACGAAG TTATGCTCGC AGACTCTTCC AGAACTTTGT CGACCCTACC TCCGTATGTT 
CAAGCTGAAA TCGAACGACG ACGGAGTCTG TACCCTGTTT TGCGACAGCA GCGTAGACTT 
GACGTTACCA TACCGCCAGC AGGTGGGACA GTGTCCGCCA TGGATCCGCT GTACTCGAGC 
CCAAAGCATC GAAAGTTACC TCGCCGCAAT AAACCTAGAC GGAGCAGACA AAATAGGAAA 
CGAAAACACA GAAGACATAA ATCGAAATGG AGGTCTACTG TTCCTACTGA TATAGTGGAT 
TCAGACTTTC AACCTACGAC TGGGACCTTT ACAACACCGT TCCCAATAAA CTCTGTAAGC 
GAACCCGAAG CCGGGTTTAT TAGAACCCCG ACTACAGCAC CGGGCCTGCA TAGACAACGA 
TCGCGGAGCG GGAGGCGGCG ACCGAAACAG AGAAGACGGA CGAAAAAAGT CCGTGGTCGG 
AAACAGAAAA CCGGTCGGCG GAACCGAAAA CGAGCAAACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis.
Nature communications54830(2014 Sep)
Racioppi C,Kamal AK,Razy-Krajka F,Gambardella G,Zanetti L,di Bernardo D,Sanges R,Christiaen LA,Ristoratore F

Neural tube patterning by Ephrin, FGF and Notch signaling relays.
Development (Cambridge, England)138(24)5429-39(2011 Dec)
Stolfi A,Wagner E,Taliaferro JM,Chou S,Levine M

FGF8/17/18 functions together with FGF9/16/20 during formation of the notochord in Ciona embryos.
Developmental biology302(1)92-103(2007 Feb)
Yasuo H,Hudson C

An integrated database of the ascidian, Ciona intestinalis: towards functional genomics.
Zoological science22(8)837-43(2005 Aug)
Satou Y,Kawashima T,Shoguchi E,Nakayama A,Satoh N

Region specific gene expressions in the central nervous system of the ascidian embryo.
Mechanisms of development119 Suppl 1S275-7(2002 Dec)
Imai KS,Satoh N,Satou Y