LOC100176643 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100176643
Entrez Gene ID	100176643
Full Name	7-dehydrocholesterol reductase
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002127536.3	XP_002127572.1	7-dehydrocholesterol reductase
XM_002127536.4	XP_002127572.1	7-dehydrocholesterol reductase

Pathways from BioSystems
Gene Ontology

The following LOC100176643 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100176643 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi01794	XM_002127536.4 Latest version!	Ciona intestinalis 7-dehydrocholesterol reductase (LOC100176643), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCi01794	XM_002127536.3	Ciona intestinalis 7-dehydrocholesterol reductase (LOC100176643), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100176643 ( XM_002127536.3 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_002127572.1 Ciona intestinalis 7-dehydrocholesterol reductase (LOC100176643), mRNA.

Price & Availability↑

CloneID	OCi01794
Clone ID Related Accession (Same CDS sequence)	XM_002127536.3 , XM_002127536.4
Accession Version	XM_002127536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	7-dehydrocholesterol reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020167.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 21, 2016 this sequence version replaced XM_002127536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTA ATGCACAAAC ACGCCACCGT GTCTCGACCA ATGGGAAACA AGGTACGACT 
TCGAATAACA ACAACATAAA AAGTTCATCG GCAACTGGCC AATGGGGCAG AGCATGGCGT 
GTGGATGTTT GGACATTCTT TGGATGTCTT TTCGTTCTGT TCGTGTGCCC AATTCTGCCG 
TTAACCTTTT ATGCATCGTG TACCCACCAC GAGTGTAGTC TGGTCGAAAC AGCTTACTCT 
GTGTGGTCCG GATCATTGGA TTTTTCTTTA CCGGCCGTTT CTGGTTTTGC CGCTCGGACA 
TATTTTACCT GGATTGCATT TCAGATTCTT CTTTCTGCAT TGCCCGACAT CGTACACTTG 
ATTCTACCAC GTTACGTTGG TGGTATTCAA CCTGGCGCAG TTACACCAAG TGGGAGGGTT 
AATAGATACA ACATCAACGG TCTTCAGGCT TGGTTAATTA CACACATACT ATGGGCGGCC 
AATGCATTTT ACTTCAAGCT TTTCTCGCCA TCCATAGTCT TCAATAACAT GGGTGCTTTC 
TTGGTGCTTT CCAACATAAC TGGATATTGT CTTGCTACGT TTTGTTACAT TAAAGGTCGG 
TTATTCCCCA CTCATGAGAA TGATTGCAAA CTAAGCGGAC ATTTCTTGTA CGATTATTTA 
ATGGGTGTTG AATTTAACCC AAGGATTGGG TGTTGGTTCG ACTTTAAATT ATTCTTTAAC 
GGTCGGCCAG GCATTGTAGC TTGGTCAATC ATCAATTTAT CATTTGCTTC GCGTCAATAC 
GAGACCTTTG GTCACGTGAC CGATTCCATG ATTCTTTTGA ATTTTCTTCA AGCAATCTAC 
GTCATTGACT TTTTCTGGAA CGAAACTTGG TATTTGAAAA CAATGGATAT TTGCCATGAC 
CATTTCGGAT TCTACCTGGC CTGGGGCGAC CTCGCGTGGC TGCCTATTAT GTATACTTTG 
CAGGGTTTTT ACCTCGCATA CCACCCGGTC GAACTCGGAG TTATAAAGTC AAGCCTCATC 
TTATTGCTAG GGTTGGTCGG TTACGTCATA TTTCGATCCG CTAACAGCCA GAAGGATAAA 
TTTCGAAAGA AGAAAAATAA ATGCAAAATT TGGGGGAAAT CTCCCGAATA TATTGAGTGT 
TCTTATACGT CATCAGACGG GGTACGTCAT CAAAGTACAC TTCTAGTATC CGGTTGGTGG 
GGTGTAGCTC GTCACTTCAA CTACACCGGA GATTTGTTAG GGTCACTTGC TTACTGCTTA 
TGTTGCGGGG GTGACCACAT TCTGCCATAT TTCTACATCA TATACATGAC CATACTGCTG 
GTACATAGGT TATATAGGGA TGAACACAGG TGTAAATGCA AGTACGGGGA GTATTGGACG 
CTCTATGTTC AACGTGTGCC TTGGAGACTT ATACCTGGCC TGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002127572.1
CDS	125..1552
Translation	MESNAQTRHRVSTNGKQGTTSNNNNIKSSSATGQWGRAWRVDVWTFFGCLFVLFVCPILPLTFYASCTHHECSLVETAYSVWSGSLDFSLPAVSGFAARTYFTWIAFQILLSALPDIVHLILPRYVGGIQPGAVTPSGRVNRYNINGLQAWLITHILWAANAFYFKLFSPSIVFNNMGAFLVLSNITGYCLATFCYIKGRLFPTHENDCKLSGHFLYDYLMGVEFNPRIGCWFDFKLFFNGRPGIVAWSIINLSFASRQYETFGHVTDSMILLNFLQAIYVIDFFWNETWYLKTMDICHDHFGFYLAWGDLAWLPIMYTLQGFYLAYHPVELGVIKSSLILLLGLVGYVIFRSANSQKDKFRKKKNKCKIWGKSPEYIECSYTSSDGVRHQSTLLVSGWWGVARHFNYTGDLLGSLAYCLCCGGDHILPYFYIIYMTILLVHRLYRDEHRCKCKYGEYWTLYVQRVPWRLIPGLY

Target ORF information:

RefSeq Version	XM_002127536.3
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis 7-dehydrocholesterol reductase (LOC100176643), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002127536.3

ORF Insert Sequence:

ATGGAGAGTA ATGCACAAAC ACGCCACCGT GTCTCGACCA ATGGGAAACA AGGTACGACT 
TCGAATAACA ACAACATAAA AAGTTCATCG GCAACTGGCC AATGGGGCAG AGCATGGCGT 
GTGGATGTTT GGACATTCTT TGGATGTCTT TTCGTTCTGT TCGTGTGCCC AATTCTGCCG 
TTAACCTTTT ATGCATCGTG TACCCACCAC GAGTGTAGTC TGGTCGAAAC AGCTTACTCT 
GTGTGGTCCG GATCATTGGA TTTTTCTTTA CCGGCCGTTT CTGGTTTTGC CGCTCGGACA 
TATTTTACCT GGATTGCATT TCAGATTCTT CTTTCTGCAT TGCCCGACAT CGTACACTTG 
ATTCTACCAC GTTACGTTGG TGGTATTCAA CCTGGCGCAG TTACACCAAG TGGGAGGGTT 
AATAGATACA ACATCAACGG TCTTCAGGCT TGGTTAATTA CACACATACT ATGGGCGGCC 
AATGCATTTT ACTTCAAGCT TTTCTCGCCA TCCATAGTCT TCAATAACAT GGGTGCTTTC 
TTGGTGCTTT CCAACATAAC TGGATATTGT CTTGCTACGT TTTGTTACAT TAAAGGTCGG 
TTATTCCCCA CTCATGAGAA TGATTGCAAA CTAAGCGGAC ATTTCTTGTA CGATTATTTA 
ATGGGTGTTG AATTTAACCC AAGGATTGGG TGTTGGTTCG ACTTTAAATT ATTCTTTAAC 
GGTCGGCCAG GCATTGTAGC TTGGTCAATC ATCAATTTAT CATTTGCTTC GCGTCAATAC 
GAGACCTTTG GTCACGTGAC CGATTCCATG ATTCTTTTGA ATTTTCTTCA AGCAATCTAC 
GTCATTGACT TTTTCTGGAA CGAAACTTGG TATTTGAAAA CAATGGATAT TTGCCATGAC 
CATTTCGGAT TCTACCTGGC CTGGGGCGAC CTCGCGTGGC TGCCTATTAT GTATACTTTG 
CAGGGTTTTT ACCTCGCATA CCACCCGGTC GAACTCGGAG TTATAAAGTC AAGCCTCATC 
TTATTGCTAG GGTTGGTCGG TTACGTCATA TTTCGATCCG CTAACAGCCA GAAGGATAAA 
TTTCGAAAGA AGAAAAATAA ATGCAAAATT TGGGGGAAAT CTCCCGAATA TATTGAGTGT 
TCTTATACGT CATCAGACGG GGTACGTCAT CAAAGTACAC TTCTAGTATC CGGTTGGTGG 
GGTGTAGCTC GTCACTTCAA CTACACCGGA GATTTGTTAG GGTCACTTGC TTACTGCTTA 
TGTTGCGGGG GTGACCACAT TCTGCCATAT TTCTACATCA TATACATGAC CATACTGCTG 
GTACATAGGT TATATAGGGA TGAACACAGG TGTAAATGCA AGTACGGGGA GTATTGGACG 
CTCTATGTTC AACGTGTGCC TTGGAGACTT ATACCTGGCC TGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100176643 ( XM_002127536.4 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_002127572.1 Ciona intestinalis 7-dehydrocholesterol reductase (LOC100176643), mRNA.

Price & Availability↑

CloneID	OCi01794
Clone ID Related Accession (Same CDS sequence)	XM_002127536.3 , XM_002127536.4
Accession Version	XM_002127536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	7-dehydrocholesterol reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020167.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_002127536.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTA ATGCACAAAC ACGCCACCGT GTCTCGACCA ATGGGAAACA AGGTACGACT 
TCGAATAACA ACAACATAAA AAGTTCATCG GCAACTGGCC AATGGGGCAG AGCATGGCGT 
GTGGATGTTT GGACATTCTT TGGATGTCTT TTCGTTCTGT TCGTGTGCCC AATTCTGCCG 
TTAACCTTTT ATGCATCGTG TACCCACCAC GAGTGTAGTC TGGTCGAAAC AGCTTACTCT 
GTGTGGTCCG GATCATTGGA TTTTTCTTTA CCGGCCGTTT CTGGTTTTGC CGCTCGGACA 
TATTTTACCT GGATTGCATT TCAGATTCTT CTTTCTGCAT TGCCCGACAT CGTACACTTG 
ATTCTACCAC GTTACGTTGG TGGTATTCAA CCTGGCGCAG TTACACCAAG TGGGAGGGTT 
AATAGATACA ACATCAACGG TCTTCAGGCT TGGTTAATTA CACACATACT ATGGGCGGCC 
AATGCATTTT ACTTCAAGCT TTTCTCGCCA TCCATAGTCT TCAATAACAT GGGTGCTTTC 
TTGGTGCTTT CCAACATAAC TGGATATTGT CTTGCTACGT TTTGTTACAT TAAAGGTCGG 
TTATTCCCCA CTCATGAGAA TGATTGCAAA CTAAGCGGAC ATTTCTTGTA CGATTATTTA 
ATGGGTGTTG AATTTAACCC AAGGATTGGG TGTTGGTTCG ACTTTAAATT ATTCTTTAAC 
GGTCGGCCAG GCATTGTAGC TTGGTCAATC ATCAATTTAT CATTTGCTTC GCGTCAATAC 
GAGACCTTTG GTCACGTGAC CGATTCCATG ATTCTTTTGA ATTTTCTTCA AGCAATCTAC 
GTCATTGACT TTTTCTGGAA CGAAACTTGG TATTTGAAAA CAATGGATAT TTGCCATGAC 
CATTTCGGAT TCTACCTGGC CTGGGGCGAC CTCGCGTGGC TGCCTATTAT GTATACTTTG 
CAGGGTTTTT ACCTCGCATA CCACCCGGTC GAACTCGGAG TTATAAAGTC AAGCCTCATC 
TTATTGCTAG GGTTGGTCGG TTACGTCATA TTTCGATCCG CTAACAGCCA GAAGGATAAA 
TTTCGAAAGA AGAAAAATAA ATGCAAAATT TGGGGGAAAT CTCCCGAATA TATTGAGTGT 
TCTTATACGT CATCAGACGG GGTACGTCAT CAAAGTACAC TTCTAGTATC CGGTTGGTGG 
GGTGTAGCTC GTCACTTCAA CTACACCGGA GATTTGTTAG GGTCACTTGC TTACTGCTTA 
TGTTGCGGGG GTGACCACAT TCTGCCATAT TTCTACATCA TATACATGAC CATACTGCTG 
GTACATAGGT TATATAGGGA TGAACACAGG TGTAAATGCA AGTACGGGGA GTATTGGACG 
CTCTATGTTC AACGTGTGCC TTGGAGACTT ATACCTGGCC TGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002127572.1
CDS	127..1554
Translation	MESNAQTRHRVSTNGKQGTTSNNNNIKSSSATGQWGRAWRVDVWTFFGCLFVLFVCPILPLTFYASCTHHECSLVETAYSVWSGSLDFSLPAVSGFAARTYFTWIAFQILLSALPDIVHLILPRYVGGIQPGAVTPSGRVNRYNINGLQAWLITHILWAANAFYFKLFSPSIVFNNMGAFLVLSNITGYCLATFCYIKGRLFPTHENDCKLSGHFLYDYLMGVEFNPRIGCWFDFKLFFNGRPGIVAWSIINLSFASRQYETFGHVTDSMILLNFLQAIYVIDFFWNETWYLKTMDICHDHFGFYLAWGDLAWLPIMYTLQGFYLAYHPVELGVIKSSLILLLGLVGYVIFRSANSQKDKFRKKKNKCKIWGKSPEYIECSYTSSDGVRHQSTLLVSGWWGVARHFNYTGDLLGSLAYCLCCGGDHILPYFYIIYMTILLVHRLYRDEHRCKCKYGEYWTLYVQRVPWRLIPGLY

Target ORF information:

RefSeq Version	XM_002127536.4
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis 7-dehydrocholesterol reductase (LOC100176643), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002127536.4

ORF Insert Sequence:

ATGGAGAGTA ATGCACAAAC ACGCCACCGT GTCTCGACCA ATGGGAAACA AGGTACGACT 
TCGAATAACA ACAACATAAA AAGTTCATCG GCAACTGGCC AATGGGGCAG AGCATGGCGT 
GTGGATGTTT GGACATTCTT TGGATGTCTT TTCGTTCTGT TCGTGTGCCC AATTCTGCCG 
TTAACCTTTT ATGCATCGTG TACCCACCAC GAGTGTAGTC TGGTCGAAAC AGCTTACTCT 
GTGTGGTCCG GATCATTGGA TTTTTCTTTA CCGGCCGTTT CTGGTTTTGC CGCTCGGACA 
TATTTTACCT GGATTGCATT TCAGATTCTT CTTTCTGCAT TGCCCGACAT CGTACACTTG 
ATTCTACCAC GTTACGTTGG TGGTATTCAA CCTGGCGCAG TTACACCAAG TGGGAGGGTT 
AATAGATACA ACATCAACGG TCTTCAGGCT TGGTTAATTA CACACATACT ATGGGCGGCC 
AATGCATTTT ACTTCAAGCT TTTCTCGCCA TCCATAGTCT TCAATAACAT GGGTGCTTTC 
TTGGTGCTTT CCAACATAAC TGGATATTGT CTTGCTACGT TTTGTTACAT TAAAGGTCGG 
TTATTCCCCA CTCATGAGAA TGATTGCAAA CTAAGCGGAC ATTTCTTGTA CGATTATTTA 
ATGGGTGTTG AATTTAACCC AAGGATTGGG TGTTGGTTCG ACTTTAAATT ATTCTTTAAC 
GGTCGGCCAG GCATTGTAGC TTGGTCAATC ATCAATTTAT CATTTGCTTC GCGTCAATAC 
GAGACCTTTG GTCACGTGAC CGATTCCATG ATTCTTTTGA ATTTTCTTCA AGCAATCTAC 
GTCATTGACT TTTTCTGGAA CGAAACTTGG TATTTGAAAA CAATGGATAT TTGCCATGAC 
CATTTCGGAT TCTACCTGGC CTGGGGCGAC CTCGCGTGGC TGCCTATTAT GTATACTTTG 
CAGGGTTTTT ACCTCGCATA CCACCCGGTC GAACTCGGAG TTATAAAGTC AAGCCTCATC 
TTATTGCTAG GGTTGGTCGG TTACGTCATA TTTCGATCCG CTAACAGCCA GAAGGATAAA 
TTTCGAAAGA AGAAAAATAA ATGCAAAATT TGGGGGAAAT CTCCCGAATA TATTGAGTGT 
TCTTATACGT CATCAGACGG GGTACGTCAT CAAAGTACAC TTCTAGTATC CGGTTGGTGG 
GGTGTAGCTC GTCACTTCAA CTACACCGGA GATTTGTTAG GGTCACTTGC TTACTGCTTA 
TGTTGCGGGG GTGACCACAT TCTGCCATAT TTCTACATCA TATACATGAC CATACTGCTG 
GTACATAGGT TATATAGGGA TGAACACAGG TGTAAATGCA AGTACGGGGA GTATTGGACG 
CTCTATGTTC AACGTGTGCC TTGGAGACTT ATACCTGGCC TGTATTAA